[关键词]
[摘要]
本研究使用克伦特罗免疫荧光层析试剂盒,基于免疫荧光层析原理,配套使用广州万孚生物技术股份有限公司研制的荧光定量检测仪,实现猪尿中所含克伦特罗的定量检测,并对试纸条的灵敏度、准确度与精密性、类似化学药物干扰、保存期等相关性能进行了评价。实验证明检测限达0.26 μg/L,与结构类似化学药物的交叉反应小于0.20%,同时验证了本试剂盒在1年的保存期限有效。整个检测过程只需20 min左右。
[Key word]
[Abstract]
Quantitative detection of the clenbuterol in pig urine was investigated with a clenbuterol kit based on immunofluorescence chromatography and a fluorescence quantitative detector. The detection limit was up to 0.26 μg/L and the cross-reaction with the chemical drugs of similar structure was less than 0.20%. And the experiment confirmed that the kit had 1-year shelf life. The total detection process just takes about 20 minutes.
[中图分类号]
[基金项目]
广东省发改委工程实验室建设项目(粤发改高技术[2010]1286号)