探究猴菇菌醇提取物对胃癌大鼠肿瘤细胞凋亡的干预效果及作用机制。选取60只SD大鼠，10只作正常组，其余50只建立胃癌模型，分为胃癌组、药物对照组、低、中、高浓度猴菇菌组，分别干预。观察各组大鼠细胞增殖、凋亡、周期分布，检测胃动素、胃泌素及Bcl-2、Bax、caspase3表达。研究结果显示，高浓度猴菇菌组大鼠胃动素、胃泌素水平分别为140.62 pg/mL、85.39 pg/mL，肿瘤组织细胞凋亡率为50.25%、G1期细胞比例为55.61%、Bax表达量为0.76，均高于模型组、药物对照组、低、中浓度猴菇菌组；高浓度猴菇菌组大鼠肿瘤组织细胞增殖率为11.52%，Bcl-2、caspase3表达分别为1.35、1.32，均低于模型组、药物对照组、低、中浓度猴菇菌组（p<0.05）。猴菇菌醇提取物能够改善胃癌大鼠胃功能，调控胃癌组织细胞增殖、凋亡及周期分布，为胃癌的临床治疗提供一定的理论依据。

To explore the effect and mechanism of ethanol extract of Hericium erinaceus on apoptosis of gastric cancer cells in rats. Sixty SD rats were selected, 10 as normal group, and the remaining 50 as gastric cancer models. They were divided into gastric cancer group, drug control group, low, medium and high concentration group of Monkey mushroom fungus. The cell proliferation, apoptosis and cycle distribution were observed, and the expressions of motilin, gastrin, Bcl-2, Bax and caspase-3 were detected. The results show that: The levels of motilin and gastrin in the high concentration group were 140.62 pg/mL and 85.39 pg/mL respectively, the apoptotic rate of tumor cells was 50.25%, the percentage of cells in G1 phase was 55.61% and the expression of Bax was 0.76, it was higher than that of gastric cancer group, drug control group, low and medium concentration of Hericium erinaceus groups. The proliferation rate of tumor cells in the high concentration group of Hericium erinaceus was 11.52%, The expressions of Bcl-2 and caspase-3 were 1.35 and 1.32 respectively, they were lower than those of gastric cancer group, drug control group, low and medium concentration of Hericium erinaceus groups (p<0.05). Ethanol extract of Hericium erinaceus can improve the gastric function of gastric cancer rats, regulate the proliferation, apoptosis and cycle distribution of gastric cancer cells, and provide a theoretical basis for clinical treatment of gastric cancer.

湖北省自然科学基金项目（2015CFC847）