本文对鳕鱼皮胶原蛋白肽（CSCP）在小鼠急性肝损伤中的保护作用进行研究。测定CSCP的急性毒性作用及最大给药剂量；建立CCl4致小鼠急性肝损伤模型，检测小鼠血清ALT、AST活性以及肝组织匀浆GSH-Px、SOD活性以及MDA含量；HE染色观察肝脏病理学改变；透射电镜观察肝脏微观结构；蛋白印迹法测定肝组织Bax、Bcl-2、Cleavage Caspase-3、TNF-α蛋白表达量。结果表明CSCP最大给药量达到8.0 g/kg，无小鼠死亡；CSCP组与模型组比较，血清中ALT、AST的活性显著降低，最高降幅达到60%以上；肝组织匀浆中SOD和GSH-Px活性显著增高，分别增加23%和29%；MDA含量显著降低，最高降幅达到38%；HE染色观察肝组织结构明显好转；透射电镜表明肝脏微观结构得到明显改善；Bcl-2蛋白表达量增加，Bax、Cleavage Caspase-3、TNF-α蛋白表达量降低。因此，CSCP对CCl4小鼠急性肝损伤有较明显的保护作用。

The protective effects of cod skin collagen peptides (CSCPs) on CCl4-induced acute liver injury in mice was examined. The acute toxicology dose and the maximal CSCP dose were determined. An acute liver injury model was established by intraperitoneal injection CCl4 in mice. The activity levels of serum ALT and AST were determined and the GSH-Px, SOD activities, and MDA content in liver homogenates were measured. Liver tissue samples of the mice were stained with hematoxylin and eosin (HE) to observe the effect of CSCP on mouse liver histopathology. Using TEM, the protective effects of CSCP on acute liver injury were examined. Expression of the Bax, Bcl-2, Cleavage Caspase-3, and TNF-α proteins were measured by western blot analysis. For the maximal CSCP dose of 8.0 g/kg, no mice died, indicating that CSCP was safe. ALT and AST activity levels in the serum samples were significantly lower in the CSCP group than the control group, and the maximal decrease was greater than 60%. Additionally, SOD and GSH-Px activity levels in liver homogenates increased significantly by 23% and 29%, respectively. The MDA content in liver homogenates significantly declined and the maximal decrease was 38%. Mouse liver tissue stained with HE revealed improvements in liver histology. The liver microstructure improved substantially based on transmission electron microscope (TEM) observations. Expression levels of Bcl-2 were higher, while the expression levels of Bax, Cleavage Caspase-3, and TNF-α were lower in the CSCP group than the control group. Taken together, CSCP effectively protects against acute liver injury by CCl4 in mice.

浙江省科技厅重大专项（2013C03036；2011C02003）；浙江省自然科学基金立项（LY13C200004，LY12C20005）；舟山市科技计划项目（2012C21013）