利用杂交瘤技术制备了诺氟沙星的单克隆抗体，建立了以抗诺氯沙星单克隆抗体为基础的荧光免疫分析方法，工作曲线表明在10~500 μg/L浓度范围内呈良好的线性关系，回归方程为：I=31.92 logC-5.0083，相关系数r=0.9965，对诺氟沙星最低检出限达6.09 μg/L，抑制中浓度为52.88 μg/L，且对其它结构类似喹诺酮类药物均有特异性识别。本法可应用于检测动物性食品中残留的微量诺氟沙星。

A monoclonal antibody (MAb) against norfloxacin was prepared using hybridom a techniques, and indirect competitive Fluorescence immunoassays for norfloxacin (NFLX) was established successfully. The regression equation was I=31.92logC-5.0083 (r= 0.9965) with the limit of detection about 6.09 μg/L. The linear detection was ranged well from 10μg/L to 500μg/L. The cross-reaction tests indicated that most structurally related phenolic compounds did not interfere with the analysis of NFLX. The anti- NFLX antibody had excellent recognition specificity to NFLX. The result developed in this study indicates this method would practicable to detection norfloxacin residues in animal derived food.

福建省科技合作计划重点项目（2011Y0035），福建省科技计划项目（2010Y2004）