Volume 40,Issue 4,2024 Table of Contents

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  • 1  Phyllanthus emblica L. Extract and Its Formulated Products Ameliorate Functional Dyspepsia Symptoms in Rats
    TAN An WANG Zhicheng YUAN Ming HU Hao CAO Yong WEN Linfeng
    2024, 40(4):1-7. DOI: 10.13982/j.mfst.1673-9078.2024.4.0462
    [Abstract](174) [HTML](62) [PDF 2.04 M](370)
    Abstract:
    In this research, small intestinal motility in mice, weight gain in rats, food intake, food utilization by rats and pepsin determination were used to examine the effects of Phyllanthus emblica L. (PEL) and four formulated products according to the digestion-promoting function test methods described in Health Food Function Inspection and Evaluation Methods (2022 edition). The results showed that the small intestinal propulsion rates of the PEL (PEL alone) group, the PEL-hawthorn (H)-Poria cocos Wolf (PCW)-Tangerine Peel (TL) group (Formulated Group 1), the PEL-H-Atractylodis Macrocephalae Rhizoma (AMR)-Hordei Fructus Germinatus (HFG) group (Formulated Group 2), the PEL-H-PCW-AMRTL group (Formulated Group 3), and the PEL-H-PCW-AMR-HFG-TL group (Forumulated Group 4) increased by 65.99%, 80.53%, 107.91%, 67.47%, and 90.47% (P<0.05, P<0.01, P<0.000 1, P<0.05, P<0.001), respectively. Their pepsin activities in rats increased correspondingly by 89.24%, 60.42%, 103.07%, 74.56%, and 106.84% (P<0.01, P≥0.05, P<0.01, P<0.05, P<0.01), and the amounts of secreted pepsin in corresponding groups of rats increased by 89.30%, 60.39%, 103.14%, 74.69%, and 106.98% (P<0.01, P≥ 0.05, P<0.01, P<0.05, P<0.01), respectively. In addition, the food utilization rates in rats of the Formulated Group 4 increased by 15.35% (P<0.05). However, there were insignificant differences in weight gain and food intakes (P<0.05) as well as the above three indicators in other groups (P<0.05). The research showed that extract of Phyllanthus emblica L. and its formulated products could effectively ameliorate the symptoms of functional dyspepsia in rats. The results of this study provides a theoretical reference for the development and promotion of digestion-promoting functional products using PEL and other formulated products.
    2  Puerarin Relieves Liver Metabolic Disorders in Mice with Nonalcoholic Fatty Liver Disease
    DING Wenjiao GE Jingjing WU You YU Yujuan ZHOU Feng RUAN Zheng
    2024, 40(4):8-17. DOI: 10.13982/j.mfst.1673-9078.2024.4.0510
    [Abstract](113) [HTML](60) [PDF 3.50 M](327)
    Abstract:
    High fat and/or high fructose intakes lead to excessive fat storage in the liver, resulting in nonalcoholic fatty liver disease (NAFLD), whose global incidence rate is 25.2% and rising rapidly. In this study, the effects of puerarin intervention on NAFLD were investigated in a mouse model induced by high-fructose and high-fat diets. The results show that the body weight and liver index of NAFLD mice decreased by 13.7% and 14.9%, respectively; the H&E staining indicated that the liver tissue damage was mitigated and the fat vacuoles almost disappeared; and the concentrations of inflammatory factors TNF-α, IL-1β, and IL-6 decreased by 62.9%, 60.5% and 61.0%, respectively, as determined by ELISA. LC-MS non-targeted metabolomic analysis of liver metabolism in the NAFLD mice showed that puerarin increased the levels of metabolites L-methionine, L-serine, L-asparagine, L-phenylalanine, and aminoadipic acid; modulated the disturbances of cysteine and methionine metabolism pathways, glycine, serine, and threonine metabolism pathways, and phenylalanine, tyrosine and tryptophan biosynthesis pathways. In addition, puerarin down-regulated the levels of the lipid metabolite oleic acid and promoted the degradation of sphingolipids and regulated the levels of pentose phosphate metabolites, vitamin metabolites, and purine and pyrimidine metabolites. In summary, puerarin regulates the disturbances of amino acid and lipid metabolism pathways, and plays a protective role against NAFLD induced by high-fructose and high-fat diets, laying an experimental foundation for dietary regulation of fat metabolism and even fatty liver.
    3  Improvement Effects of Grifola frondosa Polypeptide-Zinc Chelate on the Offspring of Zinc-deficient Pregnant Mice
    ZHANG Fengli LI Jingru PENG Peizhi HUANG Wenqi ZHAO Lina
    2024, 40(4):18-26. DOI: 10.13982/j.mfst.1673-9078.2024.4.0394
    [Abstract](83) [HTML](50) [PDF 9.37 M](358)
    Abstract:
    Grifola frondosa polypeptide-zinc chelate (GPs-Zn) is the chelation product of Grifola frondosa polypeptides (GPs) and zinc ions. GPs-Zn was subjected to structural characterization using scanning electron microscopy, X-ray powder diffraction, infrared spectroscopy, and ultraviolet spectroscopy. The effects of GPs-Zn on the offspring of zinc-deficient pregnant mice were explored by constructing an adult female ICR mouse model of zinc deficiency. Characterization results showed that GPs and zinc ions formed a novel substance through chelation; IR spectral comparison revealed that both amino and carboxyl groups on the peptide chain participated in the coordination reaction of zinc ions. The UV spectrum of GPs-Zn exhibited a red shift attributed to the binding of the carbonyl atoms of the peptides to zinc ions, indicating that GPs bound to zinc ions to form GPs-Zn. Animal experiments showed that GPs-Zn increased the thymus index of zinc-deficient offspring by 78.69% (female rats) and 87.55% (male rats), the spleen index by 40.28% (female rats) and 43.22% (male rats), the body weight by 89.98% (female rats) and 88.30% (male rats), the superoxide dismutase (SOD) activity in serum by 108.07% (female rats) and 26.16% (male rats), and the zinc concentration by 14.74% (female rats) and 29.33% (male rats), but decreased the serum alkaline phosphatase (AKP) activity by 52.28% (female rats) and 62.48% (male rats). GPs-Zn can improve the thymus index, spleen index, SOD activity, zinc concentration, and AKP activity of the offspring of zinc-deficient pregnant mice.
    4  Intestinal Loop Method Combined with Pharmacokinetics for the Investigation on the Absorption Characteristics of Compound Safflower Seed Oil
    SHANG Xueke YANG Xiaojun YANG Dongmei JING Lei ZHAO Dexiu
    2024, 40(4):27-34. DOI: 10.13982/j.mfst.1673-9078.2024.4.0482
    [Abstract](72) [HTML](71) [PDF 2.44 M](295)
    Abstract:
    In this study, the intestinal absorption characteristics of compound safflower seed oil (CSSO) were preliminarily investigated. The absorption parameters M (absorbed drug dose), P (cumulative absorption rate), Ka (absorption rate constant), t1/2 (absorption half-life) and pharmacokinetic parameters were calculated via the analysis of α-linolenic acid (ALA) and linoleic acid (LA) concentrations by liquid chromatography using an intestinal loop absorption model in combination with pharmacokinetics. The effects of drug concentration, pH value and absorption enhancers on the absorption of CSSO were investigated, as well as the absorption rate and extent of CSSO at different doses administered in animals, in order to elucidate the absorption characteristics of CSSO. The in vivo intestinal absorption experiments revealed that the M (ALA: 23.77 mg, LA: 42.27 mg) and P (ALA: 94.92%; LA: 91.89%) of ALA and LA in high concentrations of CSSO were the highest, and both increased with an elevated concentration: The M (ALA: 24.19 mg; LA: 43.38 mg) and P (ALA: 96.60%; LA: 94.295%) in CSSO were the highest at pH 8.0. The absorption enhancers had no effect on the absorption of CSSO. The pharmacokinetic experiments revealed that CSSO was absorbed rapidly, reaching the peak concentration within 1~2 h. The bioavailability was relatively high in the range of 2.604~5.208 g/kg. The results showed that the absorption characteristics of CSSO was concentration (dose)-dependent and CSSO was absorbed in a passive diffusion in rats, providing a theoretical basis for the development of unsaturated fatty acid preparations.
    5  Preparation and Comparative Analysis of Lipid-lowering Activity of Blueberry Anthocyanin Polyelectrolyte Complexes
    YIN Zhaochun LI Huantong XU Zewen CHEN Danni WANG Sainan XIAO Suyao
    2024, 40(4):35-45. DOI: 10.13982/j.mfst.1673-9078.2024.4.0321
    [Abstract](77) [HTML](58) [PDF 12.22 M](296)
    Abstract:
    To improve the stability and activity of blueberry anthocyanins, soy protein isolate, and acacia gum were used to construct and characterize soy protein isolate/acacia polyelectrolyte loaded with blueberry anthocyanins. The lipidlowering activities of the complexes were compared. Under the conditions of ultrasonic power of 180 W, a wall material quality ratio of 10:4, wall core quality ratio of 10:1, and embedding time of 1.0 h, irregular spherical particles with an embedding rate of 66.05%, a ζ-potential of 1 to 9.00 mV, and an average particle size of 2.53 μm were obtained. The 2 h-release rates of the complexes in simulated gastric and intestinal fluids were 71.05% and 61.04%, respectively, which were 19.16% and 30.50% lower, respectively, than that of free anthocyanins. A HepG2 cell model was used to determine the triglyceride (TG), total cholesterol (TC), superoxide dismutase (SOD), and malondialdehyde (MDA) content. The results showed that the lipid-lowering activity of the complex was better than that of free anthocyanins. When the mass concentration of anthocyanins was 50 μg/mL, the TC, TG, and MDA content and the activity of SOD in the complex group were 0.19 mmol/g of protein, 0.21 mmol/g of protein, and 10.58 nmol/mg of protein, which were 9.70%, 14.21%, and 17.12% lower, respectively, than the values in the free anthocyanins group. SOD enzyme activity was 25.25 U/mg of protein, which was 9.54% higher than the activity in the free anthocyanin group. The results showed that soy protein isolate and acacia can efficiently bind anthocyanins to form stable complexes, demonstrating favorable in vitro sustained release effects, and that the lipid-lowering activity of the complexes was higher than that of free anthocyanins. This study provides a theoretical basis for the subsequent development of lipid-lowering products.
    6  Passage Stability of Lactobacillus in Bile Salt MRS Medium
    XU Fan ZHANG Chenchen PAN Lina KANG Wenli WANG Jiaqi LI Wei HUANG Yujun
    2024, 40(4):46-54. DOI: 10.13982/j.mfst.1673-9078.2024.4.0382
    [Abstract](69) [HTML](62) [PDF 3.96 M](319)
    Abstract:
    In this study, a passage stability-improving method gor Lactobacillus was explored to improve the colonization ability of Lactobacillus in the gastrointestinal tract, which would provide a basis for industrial production. In this research, Lactiplantibacillus plantarun M547, M621 or M748 and Lactiplantibacillus pentosus M750 were used as the research objects to investigate the effect of bile salt addition on the growth of the strains, and the appropriate concentrations of the bile salt culture strains were determined using the growth curve instrument, and the performance of bile salt culture and MRS culture strains in terms of bile salt tolerance, acid production capacity, and acid resistance was compared. After the culture with 0.04% bile salt, the survival rate of the strain with tolerance towards 0.075% bile salt increased by about 11%~18% during passage, and the survival rate of the strain with tolerance towards 0.1% bile salt increased by 20%~34%. Bile salt culture had no obvious effect on the improvement of acid production capacity and acid tolerance and stability, but can improve the bile salt tolerance and passage stability of the strains, which can provide theoretical support for the research of the methods to improve the probiotic properties of probiotics.
    7  Effects of Cured Meat-derived Yeasts on the Quality Characteristics of Fermented Beef
    ZHANG Qiuhui MENG Gaoge WANG Han CAO Shuping CUI Wenming ZHU Chaozhi ZHAO Gaiming LI Fuqiang
    2024, 40(4):55-63. DOI: 10.13982/j.mfst.1673-9078.2024.4.1589
    [Abstract](55) [HTML](60) [PDF 2.14 M](298)
    Abstract:
    During the processing of fermented beef, Pichia diba from Xinyang cured meat and Baryces hensonensis from Sichuan cured meat were used as the fermentation strains. In order to study the effects of fermentation with a single strain on the quality of fermented beef, the local special products and blank groups without starter were used as controls, the water content, water activity (aw), pH value, protein content, nitrosamine content, texture, sensory score and other characteristic indicators of fermented beef were analyzed. The results showed that the two types of fermented beef produced with a single strain, Pichia diba or Baryces hensonensis, had the moderate hardness, with their elasticity and chewiness being significantly higher than those of traditional products. There was no significant difference in overall acceptability compared with traditional cured meat, and the water content, pH, and nitrosamine content were significantly lower than those of traditional cured meat products. Comprehensive analysis reveals that both yeasts can be used as the starters for the production of fermented meat products to improve product quality and safety.
    8  Enrichment of Thermotolerant Bacteria in Daqu and Its Application in Xiaoqu Liquor
    YING Chao REN Zhiqiang ZENG Bo HUANG Zhiguo ZHANG Yunqi WEI Chunhui
    2024, 40(4):64-72. DOI: 10.13982/j.mfst.1673-9078.2024.4.0521
    [Abstract](79) [HTML](65) [PDF 3.27 M](290)
    Abstract:
    The thermotolerant bacterial flora in Daqu contains various enzyme systems, which considerably influence the production of flavor substances in liquor. The high-temperature enrichment method was used to isolate the target thermotolerant bacterial flora from Daqu, which was subsequently incorporated into bran koji and utilized in the brewing process of Xiaoqu liquor. The functions of the Daqu thermotolerant bacterial flora in Xiaoqu liquor under three high temperature conditions were preliminarily explored. Microbial structure and diversity were analyzed using 16S rDNA sequencing, and the effect of moisture content on bacterial biomass in bran koji was investigated. The results showed that the thermotolerant bacterial flora, dominated by Bacillus, present in Daqu was successfully transferred in bran koji and became dominant. The increase in enrichment temperature within a certain range also led to an increase in the proportion of thermotolerant bacteria. When the moisture content was 55%, the bacterial biomass in bran koji was up to 8.52×1010 CFU/g. Brewing experiments using Xiaoqu liquor revealed that the optimal Xiaoqu liquor produced by brewing led to an increase in the liquor yield by 3.7%, a decrease in the content of higher alcohol by 27.27%, and an increase in the ester content by 23.07%, compared with the control group.
    9  Biological Characteristics of Limosilactobacillus reuteri K07 and Its Genetic Analysis
    HUANG Zhiyu LI Xu LI Xing JIN Tingfei TAO Chunchang LIU Zongzheng
    2024, 40(4):73-83. DOI: 10.13982/j.mfst.1673-9078.2024.4.0377
    [Abstract](69) [HTML](45) [PDF 3.32 M](320)
    Abstract:
    Two strains of Limosilactobacillus reuteri (K07, K08) were isolated from traditional fermented cheese of Zhaosu County, Xinjiang Province. The growth, acid production, acid tolerance, bile salt tolerance, tolerance to simulated gastrointestinal tract, reuterin-producing capacity and antioxidant activity of the two strains were evaluated. In addition, the bacteriostatic performance of two strains was determined and genetic analysis of the K07 strain was performed. The results showed that both strains entered the late logarithmic growth phase after 12 hours of culture, and the endpoint pH of fermentation was 4.45. K07 exhibited stronger tolerance to acid, bile salt and the simulated gastrointestinal tract as well as higher antioxidant ability than K08. The survival rate of K07 was 73.27% after the treatment at pH 2.50 for 4 h. For the K07 strain, it took 5.50 hours for the OD600 nm to increase by 0.3 units under the stress of 0.15% bile salt. The survival rate of K07 in the simulated gastrointestinal test was 74.13%. The antioxidant activities of the cell-free extracts of the two strains were higher than those of the intact cells, with the scavenging rates of the cell free extracts of K07 for DPPH free radicals and hydroxyl free radicals were 42.39% and 15.69%, respectively. with the reducing power being17.96%. The amount of reuterin produced by K07 was 183.05 mmol/L, which was significantly higher than that of K08. Also, K07 exhibited better antimicrobial performance. The diameters of inhibition zones for Candida albicans and Streptococcus agalactiae were 22.20 mm and 18.26 mm, respectively. The full length of K07 genome was 1 950 440 bp, with 2067 predicted protein coding genes. The content of GC was 38.69%. The research results showed that Limosilactobacillus reuteri K07 had good probiotic properties, which provides a theoretical basis for subsequent industrial applications.
    10  Interactions of Astaxanthin Optical Isomers with Bovine Serum Albumin
    ZHENG Qinsheng ZHOU Lesong ZHANG Junlin ZOU Xiaojun CAO Yong LIU Xiaojuan
    2024, 40(4):84-94. DOI: 10.13982/j.mfst.1673-9078.2024.4.0426
    [Abstract](66) [HTML](76) [PDF 6.31 M](306)
    Abstract:
    The mechanisms underlying the interaction between the two optical isomers of astaxanthin (AST) and bovine serum albumin (BSA) were investigated based on multiple spectroscopic, surface plasmon resonance, and molecular docking analyses. Both AST isomers were found to bind to BSA at the junction of subdomains IIA and IIIA, in the absence of any significant effects on protein conformation. The two isomers had similar binding affinities [4.17×10-7 mol/L for (3S,3’S)-AST and 3.91×10-7 mol/L for (3R,3’R)-AST for] and kinetic binding processes (slow binding, slow dissociation) for BSA. However, at higher concentrations (0.35~1.78 μmol/L), the highest binding response values obtained for (3S,3’S)-AST were higher than those for (3R,3’R)-AST. In addition, the change in enthalpy (ΔH) for the interactions between (3S, 3’S)-AST and (3R, 3’R)-AST and BSA were -175.09 and -149.42 kJ/mol, respectively, and the corresponding changes in entropy (ΔS) were -502.72 and -417.65 J/(mol•K). The negative values obtained for ΔH and ΔS indicate that hydrogen bonds and van der Waals forces were the main forces underlying the AST-BSA interactions. Molecular docking analysis revealed that (3S,3’S)-AST formed 2.0 Å and 2.7 Å hydrogen bonds with the Lys504 and Thr190 residues in BSA, respectively, whereas (3R,3’R)-AST formed a 2.9 Å hydrogen bond with the Arg435 residue. Our findings in this study contribute to elucidating the mechanisms associated with the binding of AST optical isomers to BSA, and will provide important theoretical guidance for the potential pharmacokinetics of AST isomers in blood circulation.
    11  Changes in the Antioxidant Capacity of Yunnan Camellia fascicularis during in Vitro Simulated Gastrointestinal Digestion
    SHI Zhijiao TANG Junrong XIANG Jianying KAN Huan ZHAO Ping ZHANG Guiliang LIU Yun
    2024, 40(4):95-103. DOI: 10.13982/j.mfst.1673-9078.2024.4.0423
    [Abstract](61) [HTML](64) [PDF 3.26 M](336)
    Abstract:
    To investigate the changes in the release and functional activity of total flavonoids and total polyphenols of Camellia fascicularis during the digestion, the total flavonoid and total polyphenol contents, as well as free radical scavenging ability, iron ion reducing ability, and lipid peroxidation inhibitory ability of the C. fascicularis digestive fluid were determined using an in vitro simulated gastrointestinal digestion model, and the analysis of their correlation was performed. The results showed that during simulated gastric digestion, the total flavonoid and total polyphenol contents of C. fascicularis were higher than thoseduring oral digestion, with the highest values reaching 229.99 and 327.65 μg/mL, respectively. After the intestinal digestion, the total flavonoid and total polyphenol contents were both low, ranging from 38.56 to 78.88 μg/mL, and 30.04 to 65.52 μg/mL, respectively. In the functional activity assay, DPPH and ABTS+• scavenging abilities and iron ion reducing ability were significantly higher in the gastric digestion phase than in the oral digestion, while the lipid peroxidation inhibitory ability fluctuated with the progress of digestion. After the intestinal digestion, DPPH and ABTS+•scavenging abilities and iron ion reducing ability decreased sharply after the intestinal digestion, which were lower than those during gastric digestion, whilst the lipid peroxidation inhibitory ability tended to be stable or increased gradually. The correlation analysis and principal component analysis revealed that there was certain correlations between the indicators. In summary, C. fascicularis is a better source of active substances. This study can provide a certain experimental basis and new ideas for exploring the changes of active substances and their working mechanisms during human digestion of C. fascicularis.
    12  Enzymatic Saccharification of Polygonatum kingianum Coll. et Hemsl Polysaccharides: Structural Characterization and Immunological Activity
    HUANG Junyuan YUAN Wanqing SU Yi YANG Shuting LI Pan CHEN Jianping DU Bing
    2024, 40(4):104-112. DOI: 10.13982/j.mfst.1673-9078.2024.4.0427
    [Abstract](65) [HTML](65) [PDF 3.36 M](303)
    Abstract:
    The structural characteristics and immunomodulatory activity of Polygonatum kingianum Coll. et Hemsl polysaccharides were investigated under conditions of enzymatic saccharification. The results revealed that among the products of enzymatic saccharification in P. kingianum (SPKP), the contents of polysaccharides and reducing sugars accounted for 94.05% and 4.1%, respectively. Structural analysis of the polysaccharides using Fourier transform infrared spectroscopy revealed that these had furanose-specific absorption peaks at 1 016~1 147 and 931 cm-1, whereas the monosaccharide composition and molar ratios were arabinose: galactose: glucose: mannose: fructose (0.032: 0.117: 0.419: 0.048: 0.383), and the molecular weight (Mw) was 3 820 u. The main type of linkage was →4-Glcp-1→ (47.1%), and the corresponding branching degree was 34.1%. Combined with the results of the iodine–potassium iodide and Congo Red staining experiments, SPKP were inferred to be a three-stranded helical polysaccharides containing complex branches. The findings of RAW264.7 cell experiments indicated that P. kingianum polysaccharides are non-toxic within a wide range of concentrations and can promote cell proliferation, phagocytic capacity, and production of NO. Accordingly, it can be speculated that SPKP have good immunomodulatory activities, which are associated with polysaccharide structures. Our findings in this study may provide a reference for determining the structure-function relationships between polysaccharides and immunomodulatory activity, as well as the development of new methods for processing P. kingianum.
    13  Comparison of Different Treatments and Drying Methods on the Physicochemical Properties and Antioxidant Activity of Cucumber Chips
    WANG Hao ZU Zhengmei CHENG Chenxia ZHANG Huaizhen YANG Shaolan
    2024, 40(4):113-120. DOI: 10.13982/j.mfst.1673-9078.2024.4.0478
    [Abstract](93) [HTML](52) [PDF 2.86 M](354)
    Abstract:
    Cucumber slices were subjected to steam blanching or hot water blanching pretreatment, followed by drying using three different methods: hot air frying, hot air drying, and vacuum freeze-drying. The hardness, color, rehydration, electronic nose, chlorophyll, antioxidant activity, and other physiological indices of cucumber chips were determined to explore the effects of different pretreatment and drying methods on the physicochemical properties and antioxidant activity of cucumber chips. The results showed that the hardness of unpretreated freeze-dried cucumber chips was the lowest at 0.22 N. After blanching and freeze-drying, the rehydration rate and chlorophyll content of cucumber chips were the highest at 13.37% and 1.85 mg/g, respectively. The samples treated using air fryer and hot air drying had a darker color and were prone to browning, but the color of cucumber chips could be better maintained by blanching during pretreatment. The LDA analysis conducted using the electronic nose successfully differentiated the cucumber chips subjected to different drying methods. The antioxidant capacity of unpretreated freeze-dried cucumber chips was the highest, and the DPPH• scavenging rate and O2-• scavenging rate were 66.30 and 46.12%, respectively. Thus, the color characteristics of cucumber chips could be better maintained after pretreatment and blanching, but the antioxidant activity was lower. Freeze-drying preserved the color characteristics of cucumber chips better and enhanced their nutritional value, although at higher production costs.
    14  Combination of Antibacterial Intervention and a Micro-environmental Modified Atmosphere for the Maintenance of Grape Storage Quality after Harvest
    LI Jinjin LI Chunyuan GU Baiyu JIA Xiaoyu WU Di ZHANG Peng LI Jiangkuo LUO Zheng
    2024, 40(4):121-132. DOI: 10.13982/j.mfst.1673-9078.2024.4.0226
    [Abstract](63) [HTML](51) [PDF 5.13 M](307)
    Abstract:
    To investigate the effects of antibacterial intervention combined with a micro-environmental modified atmosphere on grape preservation, ‘Sunshine rose’ grapes were used as the test material. After anti-bacterial intervention (0.8 g/L natamycin and 1.0 g/L ε-polylysine spray and 1 μL/L 1-MCP), the grapes were placed in a micro-environmental modified atmosphere box and stored in a (−0.5±0.3)℃ cold storage room. The effects of different treatment conditions on the sensory and nutritional indices of grapes were investigated, and the volatile components of grapes were analyzed using electronic nose and headspace solid-phase microextraction combined with gas chromatography-mass spectrometry. The results showed that, compared with the control group (CK), the three treatment groups delayed the decline in the storage quality of grapes to a certain extent. Among them, treatment 3 (micro-environmental modified atmosphere box+compound preservatives+1-MCP) created a gas environment more conducive to inhibiting the respiration of grapes. After 90 days of storage, the fruit decay rate, drop rate, and weight loss rate were 6.54%, 1.70%, and 0.30%, respectively; they were maintained at a relatively low level. This effectively inhibited the decrease in the L* value, fruit hardness, Vc, and soluble solid and titratable acid contents. The analysis of the volatile components showed that the flavor components of grapes in the three groups changed markedly during storage, and treatment 3 was more beneficial to the release and maintenance of aromatic components of grapes. In conclusion, treatment 3 effectively delayed the ripening and senescence of ‘Sunshine Rose’ grapes and had the best preservation effect.
    15  Comparison of the Preservation Effects of Different Packaging Films on White Mushroom
    ZOU Youhua ZHAI Mengjie LIAN Lingdan LI Yanzeng LI Weipeng LI Chen WANG Jie
    2024, 40(4):133-141. DOI: 10.13982/j.mfst.1673-9078.2024.4.0495
    [Abstract](71) [HTML](67) [PDF 2.71 M](296)
    Abstract:
    The effects of three packaging films (PE, PE+microporous, and PP) on the storage quality of Pleurotus ostreatus were studied, and the mechanism underlying such effects was explored. The results showed that the treatment with the polytene (PE) packaging film led to the lowest browning degree and the best appearance quality, on the 12th day of storage, the contents of soluble protein and reducing sugar increased by 21.39% and 70.68% respectively, with the total sugar content being the highest, and the weight and hardness decreasing by 0.82% and 1.15% respectively. Also, the treatment with the PE film resulted in the lowest conductivity and malondialdehyde (MDA) content, decreases in the activities of chitinase (CHT) and β-1,3-glucanase (Glu) by 46.87% and 48.56%, respectively, and the highest activities of superoxide dismutase (SOD) and catalase (CAT), with the CAT activity, content of antioxidant flavonoids, content of total phenols, and lignin content increasing by 2.42, 1.62, 8.18 and 1.20 times, respectively. Although the PE+microporous treatment enhanced the activity of SOD and the formation of antioxidant substances in the later stage of storage, the effect of delaying browning was poor. The browning, oxidation and weight loss were the most severe in the PP treatment group. Insummary, the treatment with PE packaging film has the best storage effect. It may enhance its antioxidant capacity by delaying the degradation of cell wall, and increasing the activity of antioxidant enzymes and the content of antioxidant substances. The results of this study can provide certain reference and a basis for the post-harvest storage and preservation technology of Pleurotus ostreatus.
    16  Physiological Comparison of Plum Fruit Quality at Different Storage Temperatures
    WAN Xuan LIN Xin PENG Junsen ZHANG Qin LUO Dengcan DONG Xiaoqing
    2024, 40(4):142-155. DOI: 10.13982/j.mfst.1673-9078.2024.4.0213
    [Abstract](81) [HTML](60) [PDF 5.45 M](321)
    Abstract:
    To explore the suitable postharvest storage temperatures of two plum fruits, “FTL” and “KXL”, their appearance and intrinsic quality indices were measured at 0, 2, and 4 ℃ . The results showed that low temperatures could contribute to the maintenance of plum fruit hardness to a greater extent while concurrently mitigating respiration, thus maintaining overall fruit quality. Among them, the soluble solids of “FTL” accumulated rapidly at 4 ℃ and the respiration rate increased. The color turned from green to yellow, resulting in a loss of market value. However, temperatures of 0 and 2 ℃ effectively inhibited the respiration rate of“ FTL” and delayed the consumption of nutrients, but the weight loss of the fruit was pronounced at 2 ℃, superoxide dismutase activity at the early storage stage was significantly lower than that at 0 ℃, which was conducive to maintaining favorable color and delaying the decrease in hardness, inhibiting the weight loss rate, and increasing the content of soluble solids. During the storage process of“ KXL”, the decrease in fruit hardness and the soluble solid content was relatively stable at all three temperatures, but 0 ℃ was more favorable than 2 and 4 ℃ for maintaining fruit hardness, slowing down the weight loss rate, increasing the respiration rate, and accumulating malondialdehyde and hydrogen peroxide. The color of the fruit remained favorable at 0 ℃; however, the fruit lost its unique color in the later stage of storage at 2 and 4 ℃ . In summary, 0 ℃ storage was conducive to maintaining the appearance and intrinsic quality of “FTL” and “KXL”, thereby extending the shelf life.
    17  Preparation and Characterization of Gallic Acid-phospholipid Complex and Its Effect on the Oxidative Stability of Walnut Oil
    DU Yihan HE Qiang DONG Yi
    2024, 40(4):156-166. DOI: 10.13982/j.mfst.1673-9078.2024.4.1068
    [Abstract](76) [HTML](58) [PDF 4.32 M](297)
    Abstract:
    Gallic acid possesses various biological activities; however, its poor lipid solubility limits its application. In this experiment, gallic acid and soy lecithin were used for the preparation of gallic acid-phospholipid complexes with the solvent evaporation method. The optimal conditions for the preparation of the gallic acid phospholipid complexes were optimized using one-factor experiments. The complexes were structurally characterized by ultraviolet absorption spectroscopy, infrared spectroscopy, and differential scanning calorimetry to verify their formation. The oil-water partition coefficients and the antioxidant properties were also measured. The optimal preparation process was as follows: using anhydrous ethanol as the solvent, a gallic acid concentration of 1 mg/mL, a molar feeding ratio of gallic acid to phospholipids of 1:4, and constant temperature stirring at 45 ℃ for 2 h. The average composite rate of the gallic acid-phospholipid complex prepared by this method was 94.65%. The oil-water partition coefficient of the gallic acid-phospholipid complex significantly improved, exhibiting favorable in vitro antioxidant activity that did not significantly differ from that of gallic acid alone. Accelerated oxidation experiments were performed on walnut oil using the gallic acid-phospholipid complex to determine the effect of the complex on the oxidative stability of walnut oil. The results showed that the gallic acid-phospholipid complex could effectively inhibit the oxidation of walnut oil, exhibiting a significantly superior antioxidant effect compared to gallic acid within 12 d (P<0.05). The gallic acid-phospholipid complex prepared in this study had a high composite rate, favorable fat solubility and antioxidant activity, and could effectively improve the oxidative stability of walnut oil.
    18  Effects of Glycerol Plasticizer Concentration on the Properties of Hydroxypropyl Starch-agar Composite Soft Capsule Shells
    CAI Xingzhe YU Long HU Kaizhen CHEN Jiayi LIU Hongsheng MIAO Chengdu
    2024, 40(4):167-176. DOI: 10.13982/j.mfst.1673-9078.2024.4.0537
    [Abstract](80) [HTML](58) [PDF 4.91 M](337)
    Abstract:
    In order to identify a suitable substitute for traditional gelatin soft capsule shells, a series of composite soft capsule shells with different glycerol concentrations were prepared based on a casting method, using hydroxypropyl starch (HPS) and agar (AG) as raw materials and glycerol (G) as a plasticizer. The effects of glycerol concentration on the properties of the composite capsule shells were investigated. Fourier transform infrared spectroscopic analysis revealed hydrogen bond interactions between HPS and AG in the composite capsule shell, whereas the findings of thermogravimetric analysis indicated that the addition of glycerol reduces the compatibility between HPS and AG, thereby leading to an increase in the thermal stability of the composite capsule shells with an increase in glycerol concentration (0~20%, m/m). Absorbance of the composite material at 600 nm initially increased and subsequently declined with increasing glycerol concentration, with maximum absorbance (0.60) being recorded for composite capsule shells with a 10% (m/m) glycerol concentration (10% G+HPS+AG), thus indicating good opacity. Similarly, the water contact angle (WCA) of the composite capsule shells initially increased with increasing glycerol concentration prior to a subsequent decline, with the highest value (86.17°) being obtained for the 15% G+HPS+AG composite capsule shells, indicating good hydrophobicity. The 15% G+HPS+AG composite capsule shells also had good Young’s modulus (17.47 MPa), tensile strength (3.17 MPa), and elongation at break (94.17%), thereby meeting the requirements for stable production. Compared with the traditional gelatin soft capsule shells, the 15% G+HPS+AG composite shells were softer under dry storage conditions and had good storage stability. Moreover, given that the raw materials are of non-animal origin, this would ensure better safety. Our findings indicate that the HPS-AG composite system has promising application prospects in the field of soft capsule shell production, and will provide a theoretical foundation for the industrial manufacture of starch-based soft capsule shells.
    19  Preparation and Properties of Soybean-nut Cheese Analogs
    CHEN Douyi QIU Xiaomin LIN Meiyun ZHENG Qianwang YE Zhiwei GUO Liqiong LIN Junfang ZOU Yuan
    2024, 40(4):177-185. DOI: 10.13982/j.mfst.1673-9078.2024.4.0430
    [Abstract](75) [HTML](66) [PDF 3.23 M](320)
    Abstract:
    A novel cheese analog was prepared using soybean milk, cashew milk, and almond protein as raw materials. The best formula of soybean-nut cheese analogs with high quality was prepared, and their physical and chemical properties, sensory properties, storage stability, and digestive properties were evaluated. The results show that the formula of soybean-nut cheese analogs with the best quality was as follows (all in mass fraction): cashew milk 50.00%, and soybean milk 50.00%. Based on the mass of plant milk, the following were added: vegetable oil: 3.09% (coconut oil and sunflower oil in a ratio of 7:3), almond protein: 2.01%, TG: 0.33%, citric acid: 0.10%, and lactic acid: 0.10%. Based on the curd mass, 1.08% colloid and 4.00% sucrose were added. The hardness of the soybean-nut cheese analog was 0.88 N and the overall sensory score was 7.71, which are comparable to those of commercial animal-based cheese. In addition, with an increase in storage time, the soybean-nut cheese analog hardness increased from 0.94 N to 1.15 N, with no significant hardening or water separation. The results of the in vitro digestion profiles of cheese analogs showed that the protein bands disappeared completely after 180 min intestinal digestion, and the release of free fatty acid was relatively low at 60%. Therefore, the prepared soybean-nut cheese analogs have high nutritional value, good sensory quality, good storage stability, and easily digestible protein, and can effectively serve as a substitute for animal-based cheese, meting the cheese preferences of consumers.
    20  Effect of Exogenous Amino Acid Addition on the Batter System of Spring Roll Wrappers
    MA Ruyue ZHONG Xiyang WANG Jialin SUN Mengjin LUO Shuizhong ZHAO Yanyan ZHENG Zhi
    2024, 40(4):186-195. DOI: 10.13982/j.mfst.1673-9078.2024.4.0451
    [Abstract](62) [HTML](69) [PDF 5.50 M](272)
    Abstract:
    Amino acids, as good nutritional and flavor enhancers, have been rarely explored in the batter system of spring roll wrappers. Eight amino acids from four categories (basic amino acids: Arg, Lys; acidic amino acids: Glu, Asp; neutral amino acids: Thr, Gly; non-polar amino acids: Trp, Met) were added into the batter system of spring roll wrappers, and the effects of their addition on the rheological and thermodynamic characteristics, microstructure, and secondary structure of the gluten protein were investigated. The results showed that the addition of basic and acidic amino acids increased the viscosity of the batter, reduced the water-separating proportion of the batter, and decreased the content of free water in the batter system. In addition, the batter strength of the gluten network structure increased, demonstrating a tighter connection. The Arg in the basic amino acids reduced the energy enthalpy required for starch pasting in flour slurry (1.68 to 1.24 J/g). Moreover, with the addition of acidic amino acids, the energy required for the gelatinization of flour starch in batter increased, and the content of the β-folded structure decreased, along with an increase in the content of the α-helix and random coiling structures in the secondary structure of gluten. The addition of neutral and non-polar amino acids decreased the viscosity of the batter, increased the water-separating proportion of the batter, had little influence on the thermodynamic characteristics of the batter system, and increased the content of the β-fold and β-corner structure in the secondary structure of the gluten protein. This study provides basic knowledge on the application of amino acids in wheat flour products.
    21  Analysis of Quality Properties of Popping Candy with Different Particle Sizes
    ZENG Shaowen LI Biansheng RUAN Zheng LI Dandan HONG Yonghong WU Jie
    2024, 40(4):196-205. DOI: 10.13982/j.mfst.1673-9078.2024.4.0371
    [Abstract](59) [HTML](71) [PDF 3.94 M](180)
    Abstract:
    Popping candy is a type of hard candy containing high-pressure CO2 bubbles, which can produce a pleasant popping sensation when dissolved. Due to the small particle size of popping candy, it is not easy to directly measure its air pressure. In addition, its irregular shape and uneven sizes pose certain challenges in the measurement of indicators. There is currently no relevant standard to evaluate the quality properties of popping candy. In this study, popping candies were divided into four particle size grades: 10 mesh
    22  Comparative Analysis of Ultra-high Pressure and Thermal Sterilization of A Blueberry-strawberry-apple Puree
    MA Xinyu BI Xiufang REN Shuning XING Yage
    2024, 40(4):206-214. DOI: 10.13982/j.mfst.1673-9078.2024.4.0402
    [Abstract](62) [HTML](53) [PDF 3.16 M](343)
    Abstract:
    Using blueberries, strawberries and apples as the raw materials, a blueberry-strawberry-apple compound puree was developed through sensory evaluation. The puree was treated by ultra-high pressure (UHP) and thermal sterilization (TS), respectively. The changes in microorganisms, physical and chemical indicators, color, content of anthocyanins, aroma and rheological properties of the puree before and after the treatment and during 40 days of storage at 4 ℃ were analyzed. The results showed that the total number of bacterial colonies, coliform and mold were not detected in the purees immediately after the two treatments, and the counts of these microorganisms still met the food safety standard limit after 40 days of the storage. The pH decreased significantly after either treatment and during the storage (P<0.05). The total soluble solids did not change significantly after UHP (P>0.05), but increased significantly after TS (P<0.05), with both reaching 7.80 °Brix at the end of storage. After TS, the anthocyanin content in the puree decreased significantly (P<0.05), with insignificant change in the UHP treated samples (P>0.05). During the storage, the anthocyanin content of the UHP treated puree was significantly higher than that of TS group (P<0.05). The maximum ΔE values of UHP and TS were 1.26 and 3.47, respectively, indicating that TS could cause significant color changes in the puree during storage. The results of the electronic nose testing showed that UHP could better retain the aroma components of the puree than TS. The analysis by the rheometer showed that the puree after UHP had better stability during storage. In summary, UHP could better maintain the quality of the blueberry-strawberryapple puree, which was a proper sterilization method for the processing of the puree.
    23  Quality Comparison of Vacuum Freeze-dried Phyllanthus emblica after Ultrasound-assisted Osmotic Treatment with Different Small Molecular Sugars
    PENG Jinming LIANG Guiqiang XIAO Yushen WEN Wenjun QIU Yuanxin WANG Qin XIAO Gengsheng
    2024, 40(4):215-224. DOI: 10.13982/j.mfst.1673-9078.2024.4.0509
    [Abstract](55) [HTML](63) [PDF 6.26 M](148)
    Abstract:
    The physicochemical properties and antioxidant activities of freeze-dried Phyllanthus emblica (FDP) obtained by ultrasound-assisted osmotic treatment with five types of small molecular sugars were evaluated. The results showed that the water loss rate and the solid content rate increased, and the internal pores became denser, with significantly increased hardness and maintained brittleness, but decreased rehydration rate compared with the control group. The hygroscopic rate of the isomalto-oligosaccharide treatment group (18.25%) was lower than that of the control group (22.54%). The ΔE of the glucose and maltose treatment groups was 2.73 and 1.71, respectively, indicating that they exhibited potent color protection. Isomalto-oligosaccharide and sucrose treatment had a flavor-strengthening effect on FDP. The sucrose, glucose, and maltose treatments reduced the titrable acid content of FDP. The glass transition temperature (Tg) of the maltose treatment group increased to 45.53 ℃, whereas the Tg of other treatment groups significantly decreased compared with the control group (43.66 ℃). Except for the xylitol treatment group (9.44 mg/g), the total phenol retention rate was high in the different treatment groups. The antioxidant capacity of FDP was maintained by sucrose and isomaltose osmosis. In conclusion, the quality and antioxidant activity levels of FDP treated using ultrasound-assisted sucrose and isomalto-oligosaccharide osmosis were higher than those of other treatments. Thus, isomalto-oligosaccharide emerges as a viable substitute for sucrose in the development of functional lyophilized P. emblica.
    24  Optimizing the Subcritical Extraction Process of Amber Essential Oil and Analysis of Its Functional Activity
    YANG Yuanyuan TANG Yuqian YANG Jiguo
    2024, 40(4):225-235. DOI: 10.13982/j.mfst.1673-9078.2024.4.0406
    [Abstract](76) [HTML](62) [PDF 5.97 M](136)
    Abstract:
    Subcritical fluid extraction of amber essential oil was conducted using amber as the raw material. The extraction process was optimized using extraction yield as the metric. The extracted amber essential oil was subjected to GCMS analysis to determine the major chemical components and their relative contents, and also subjected to in vitro antioxidant analysis and tyrosinase inhibition tests. The results show that the optimal process conditions were a liquid-to-material ratio of 2.1:1 (mL:g), an extraction temperature of 58 ℃ , and an extraction duration of 51 min, which led to an extraction yield of 1.61%. The GC-MS analysis revealed that 40 peaks were isolated from the amber essential oil, with 30 compounds being identified. Among them, monoterpenes were the most abundant compounds, mainly comprising cymene (29.39%), (-)-borneol (18.02%), fenchol (8.46%), (-)-camphor (7.12%), and eucalyptol (6.40%), which are the main contributors to the unique aroma of amber essential oil. The amber essential oil showed strong antioxidant activity, with IC50 values of 12.66 mg/mL and 2.91 mg/mL for the scavenging of DPPH and ABTS+ radicals, respectively. The inhibition of tyrosinase by amber essential oil is noncompetitive, with an IC50 value of 1.13 mg/mL, and its whitening activity is higher than that of arbutin at the same concentration.
    25  Analysis of the Relationship between Protein Denaturation and Quality of Traditional Cantonese-style Roasted Duck in the Blanching Process
    YANG Haihua LIN Jin ZHENG Hua WU Xiangheng WU Shaozong GUO Zonglin LIN Jiahui LI Zihang LIN Jie
    2024, 40(4):236-244. DOI: 10.13982/j.mfst.1673-9078.2024.4.0558
    [Abstract](70) [HTML](67) [PDF 5.61 M](156)
    Abstract:
    The blanching process of Cherry Valley ducks was investigated by observing the temperature changes during blanching and the variations in moisture content, color, protein denaturation, and microstructure before and after blanching. The results showed that blanching of duck skin at 90~95 ℃ for 5 s achieved the desired sensory requirements for traditional crispy skin, in which the surface temperature of the duck skin was over 80 ℃ and the internal temperature surpassed 45 ℃. The water loss rate during the air-drying process was not significantly different (P>0.05), which was in accordance with the results of protein denaturation measured by differential scanning calorimetry (DSC), indicating protein denaturation and aggregation at this temperature. Further investigation of the 90 ℃ blanching condition, showed that blanching at 90 ℃ for 7 s (90 ℃-7 s) followed by air-drying for 3 h (moisture, 22.01%) led to superior puffing skin compared to blanching at 90 ℃ for 9 s (90 ℃-9 s), followed by air-drying for 4 h (moisture, 20.52%). The DSC data also revealed that the protein thermal aggregation degree under 90 ℃-7 s was greater than that under 5 s blanching (P<0.05), whereas 90 ℃-9 s resulted in a gel-like protein structure. In conclusion, the key to achieving the crispy and puffed skin of traditional Cantonese-style roasted duck relies on the control of protein denaturation and aggregation of the duck skin after blanching, as well as the moisture content in this state. The findings of this study provide valuable insights and techniques for understanding the blanching mechanism and improving the skin-blanching process of Cantonese-style roasted duck.
    26  Effect of Pre-processing and Drying Techniques on Physical, Bioactive Compound, and Antioxidant Properties of Sedum aizoon L.
    YANG Liyang LU Qi YANG De WANG Yi WANG Qiong GUO Peng XUE Shujing
    2024, 40(4):245-256. DOI: 10.13982/j.mfst.1673-9078.2024.4.0242
    [Abstract](76) [HTML](66) [PDF 5.90 M](187)
    Abstract:
    To evaluate the processing adaptability of Sedum aizoon L. and expand its utilization, five pretreatment techniques (untreated [UT], boiling water blanching [BWB], color protection+water blanching [CPB], steam blanching [SB], and freeze thawing [FT]), followed by two drying methods (hot-air drying [HA], and heat-pump drying [HP]) were used. Ten types of water spinach powders, namely UT HP, UT HA, BWB HP, BWB HA, CPB HP, CPB HA, SB HP, SB HA, FT HP, and FT HA, were obtained. Changes in color, bioactive compound levels, and antioxidant capacities were compared. UT HP had the highest content of gallic acid and kaenaphthol, but had low color brightness, low chlorophyll content, and a longer drying time. CPB HP had a shorter drying time, the highest Vc content, and relatively high antioxidant levels, but lower gallic acid content. The contents of total phenols, total flavonoids, and quercetin were the highest in SB HP, whereas other quality indices fell within intermediate ranges. FT HP had the best color, the highest content of chlorophyll, and the highest free amino acid content, but lower contents of total phenols, total flavonoids, quercetin, and kaempaphthol, and a lower antioxidant capacity. Principal component analysis revealed similarities in water spinach quality among BWB HP, CPB HP, SB HP, BWB HA, CPB HA and SB HA, but there were distinct differences between UT HP, FT HP, UT HA, and UT HA. This study comprehensively compared the advantages and disadvantages of different pretreatments and drying techniques for S. aizoon. The findings of this study can serve as a valuable reference for guiding the selection of drying techniques in the development of functional foods.
    27  Analysis of the Volatile Components of Traditional Liupao Tea by using Adsorption Extraction Combined with Gas Chromatography-tandem Mass Spectrometry
    LIANG Jianfeng LI Ya BIN Yuejing XI Guangsheng CHEN Jinhui LIANG Lili
    2024, 40(4):257-263. DOI: 10.13982/j.mfst.1673-9078.2024.4.0543
    [Abstract](67) [HTML](56) [PDF 5.38 M](137)
    Abstract:
    To explore the volatile components and key aromatic substances of traditional Liupao tea, the gas chromatography-mass spectrometry technique combined with relative odor activity value and flavor wheel techniques was used to analyze three traditional Liupao tea samples. In total, 39 aromatic compounds were identified from six categories of aromatic hydrocarbons, alcohols, ketones, aldehydes, alkenes, and heteroxy compounds. The volatile components and relative contents of samples significantly differed. Nongjia Liupao tea contained high alcohol (62.66%) and oleic (26.64%) volatile compound contents, Liupao tea fruit contained high alcohol (81.10%) and aldehyde (3.62%) volatile compound contents, and Liupao old tea contained high alcohol (56.13%) and oleic (36.95%) volatile compound contents. α-cedarol exhibited the highest relative content of volatile components (38.92%~49.12%) in traditional Liupao tea samples. α-cedarol, vedranol, allocedarol, and β-linalool contents were determined by the ROAV values of the samples as volatile components (ROAV>1.00); citronellal, trans-2-octenal, 2-n-amylfuran and other seven compounds had modification effects on the aroma of samples (0
    28  Comparative Analysis of Differential Flavonoid Metabolites in Red Beetroot
    BAI Wenyu LIU Naixin HAN Guangyuan YU Qingtao ZHOU Qin CHEN Baicui LI Chengxin
    2024, 40(4):264-276. DOI: 10.13982/j.mfst.1673-9078.2024.4.0216
    [Abstract](64) [HTML](57) [PDF 3.38 M](143)
    Abstract:
    In this study, poinsettias and Russian red beet roots were used as the raw materials, and the flavonoids in the two kinds of red beet roots were detected and the composition and differences of their metabolites were analyzed using the UPLC-MS/MS detection platform and the self-built Metware database. Multivariate statistical analysis was used to screen the differential metabolites based on the data of the detected flavonoid metabolites. Then, the KEGG website was used to analyze the synthetic pathways involved in differential metabolites. The results showed that a total of 69 flavonoid metabolites were detected in two kinds of beet roots, which were divided into six types: flavonoids, flavonols, chalcones, dihydroflavones, auronoids and other flavonoids. There were 29 kinds of differential metabolites, with 12 kinds of differential metabolites having higher contents in poinsettia than in Russian red, and 17 kinds having lower contents in poinsettia than in Russian red. According to the metabolite, log2FC, the components with relatively large differences were mainly pentahydroxy chalcones, isorhamnetin, dimethoxy-flavone, anthrone, polymethoxy-flavone, quercetin, kaempferol, geranyl, and hyperplantin derivatives. There were seven metabolites annotated to the KEGG pathway: 3-O-methylquercetin, luteolin, 3'-o-methylluteolin, rutin, isoquercetin, isovitexin, and trilobin. These results indicated that there were significantly different flavonoid metabolites in two kinds of beet substances, which could be used as characteristic metabolites for further analysis.
    29  Comparative Analysis of Flavor Quality during the Fermentation of Dried Petals and Raw Petals
    JIANG Siqiang DENG Weiqin FAN Zhiyi LI Xiongbo WANG Zeliang LI Heng CHEN Gong
    2024, 40(4):277-286. DOI: 10.13982/j.mfst.1673-9078.2024.4.0369
    [Abstract](55) [HTML](60) [PDF 3.52 M](148)
    Abstract:
    To explore the effect of the fermentation process on the quality of broad bean paste-meju (BBPM), in this study, the microorganisms, flavor substances, color differences, textural characteristics and other indexes of the dried petals and raw petals were compared and analyzed. The results showed that the quality of the dried petals and raw petals differed significantly (P<0.05). The number of molds and bacteria in the fermentation process of the two BBPMs differed significantly. The numbers of bacteria and molds in the dried petals and raw petals at the end of the fermentation were 5.07 and 4.81, and 2.75 and 2.13 lg CFU/g, respectively. The total acid and amino acid nitrogen contents of the two BBPMs increased significantly during the fermentation process, with the total acid of the raw petals (1.81 g/100 g) being significantly higher than that of the dried petals (1.48 g/100 g), while the hardness of the raw petals (30.69 N) being higher than that of dried petals (14.48 N); The amino acid nitrogen (0.69 g/100 g) in the dried petals was significantly higher than that of the raw petals, with a redder and brighter color. The flavor analysis results showed that the flavor of the dried petals was more prominent, and the flavor of the raw petals was relatively harmonious. A total of 57 kinds of flavor substances were detected in the raw petals and the dried petals, and the compositions of flavor substances of the two BBPMs resembled. The relative contents of the same flavor substance in the two sweet petals were quite different, and the dried petals contain a greater variety of flavor compounds and higher relative contents, with the 14 kinds of substances, including 2-methylbutanol, isoamyl acetate, ethanol, acetic acid and isovaleraldehyde, being the main differential flavor compounds. The results of sensory evaluation showed that the quality of dried petals was better than that of raw petals. In summary, the dried petals had a short fermentation cycle and more prominent flavor, which is suitable for the production of modern BBPMs.
    30  Development of an Insulated Isothermal PCR-based Method for Rapid Detection of Bacillus cereus
    KOU Xiaodi MENG Han SU Yahang TANG Cheng HU Yu TANG Junni
    2024, 40(4):287-295. DOI: 10.13982/j.mfst.1673-9078.2024.4.0230
    [Abstract](66) [HTML](74) [PDF 4.01 M](136)
    Abstract:
    Specific primers and probe were designed for the gyrB gene of Bacillus cereus encoding the gyrase B subunit, and a method based on insulated isothermal polymerase chain reaction (iiPCR) was established for the rapid detection of B. cereus and subsequently validated. The efficacy of this method was compared with that of traditional PCR and real-time fluorescent quantitative PCR (SYBER Green I fluorescent dye and TaqMan probe methods) detection methods, and applied in the detection of B. cereus in different types of retail food. The results revealed that the newly developed method facilitated the rapid detection of B. cereus within 40 min. Moreover, there was no cross reaction with other bacteria, including Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, and Salmonella enteritidis, thereby indicating good specificity. The minimum detection limit of the method was 1.5×102 CFU/mL, which was significantly superior to that of the traditional PCR method, and equivalent to that of quantitative PCR. Of the 42 retail food samples assessed, 19 (45.23%) were found to be contaminated with B. cereus. The results obtained for method validated were completely consistent with those obtained using traditional PCR detection, although test results were available within a considerably shorter timeframe, saving at least 4 h. The method developed in this study provides a rapid, convenient, specific, and sensitive procedure that is suitable for the real-time on-site detection of B. cereus.
    31  Rapid SDS-PAGE Fluorescent Detection Method for Lactoferrin Content in Milk and Dairy Products
    LI Hanfang HUANG Zhen LU Mengfan SUN Nana XU Dan XU Qinfeng
    2024, 40(4):296-302. DOI: 10.13982/j.mfst.1673-9078.2024.4.0968
    [Abstract](73) [HTML](52) [PDF 3.55 M](143)
    Abstract:
    A novel dye (Chromeo P503, P503) was used as an alternative to Coomassie brilliant blue to address the drawbacks associated with the laborious and time consuming (≥5 h) staining and destaining processes. This facilitated the establishment of a rapid and simple sodium dodecyl sulfonate-polyacrylamide gel electrophoresis (SDS-PAGE) method for the detection of lactoferrin in milk and dairy products. P503 dye-labeled protein emits strong red fluorescence, which could be completed in one step within 30 min. The interference of lactoferrin iron saturation on fluorescence signal was eliminated after apolactoferrin preparation to ensure the accuracy of quantification. Qualitative and quantitative detection of lactoferrin was achieved based on the presence of a 78 ku protein band and its gray value in the gel image. Under optimized experimental conditions, the linear range was 15~75 μg/mL, and the limit of detection was 5.11 μg/mL, with a recovery of 75.79%~108.09%, indicating that this method has high sensitivity and accuracy. The combination of the SDS-PAGE and P503 methods enabled the rapid, simple, and accurate detection of lactoferrin in milk and dairy products, eliminating the need pretreatment of the heparin affinity column. P503 dye labeling can facilitate rapid and low-cost quantitative detection of other milk proteins using SDS-PAGE, providing a valuable tool for dairy quality control, nutrition evaluation, and processing technology research.
    32  Residue Analysis and Dietary Risk Assessment of Plant Growth Regulators in Melons of Different Origins
    XI Zhengqi SHEN Qi WUJISIGuleng HE Weizhong WANG Cheng
    2024, 40(4):303-311. DOI: 10.13982/j.mfst.1673-9078.2024.4.0513
    [Abstract](58) [HTML](59) [PDF 1.73 M](137)
    Abstract:
    In order to clarify the residue status of plant growth regulators in melons from different production areas and to explore the residue risk, in this study, 132 melon samples from Guangxi, Hainan, Hebei, Liaoning, Jiangsu and Jilin in 2020 were used as the research objects. Quantitative analysis of the residue levels of six plant growth regulators in these samples was carried out with reference to DB65/T 3970-2017 "Determination of residues of 17 plant growth regulators in vegetables and fruits by liquid chromatography-tandem mass spectrometry". The differences in residues of plant growth regulators in the melons from different production areas were compared, and then the acute and chronic risk assessment and risk ranking fordietary intake of residual plant growth regulators in the melons from different production areas were carried out. There were 5 kinds of plant growth regulators left in the melon samples, among which abscisic acid residues accounted for the highest proportion (72.13%), followed by paclobutrazole, chlorpyramide, and enbutrazole (accounting for 11.48%, 9.29%, 4.37% and 2.73%, respectively). In terms of the five residual plant growth regulators, the chronic risk of their dietary intake ranged from 0% to 0.119 7%, with an average value of 0.015 2%; The acute risk of their dietary intake ranged from 0% to 9.714 1%, with a mean value of 0.669 2%. Both risks were well below 100% and at a safe level. Risk ranking score revealed that there were no high-risk plant growth regulators, and the risk scores of residual samples from different origins were different. Based on the proportion of residual samples, acute and chronic dietary risk assessment and risk scores, eMRL recommends to formulate and revise the maximum residue limits of paclobutrazole, polycarboxylin and enbutrazole to 9, 4 and 1 mg/kg, respectively, and the residue risks of abscisic acid and paclobutrazole are relatively high. Thus, it is recommended to pay more attention.
    33  Safety Risk Assessment of Guangzhou’s Meat and Meat Products Based on GRA-ISM-HMM
    ZHANG Weiwei CHEN Kuncai ZHANG Yuhua CHEN Yanshan HUANG Deyan
    2024, 40(4):312-320. DOI: 10.13982/j.mfst.1673-9078.2024.4.0473
    [Abstract](55) [HTML](72) [PDF 2.71 M](151)
    Abstract:
    Using the food safety risk monitoring data of meat and meat product samples in Guangzhou from 2015 to 2020, a safety risk assessment model for meat and meat products was constructed to understand the food safety risks and time-varying characteristics of meat and meat products in Guangzhou during this period. The gray correlation analysis method and the explanatory structure model are adopted to establish the risk index, and the implied risk state behind the observation is explored based on the value of this index as the observation of the Hidden Markov Model. The analysis results show that the results of the composite risk index for all samples from 2015 to 2020 are between [0, 0.45], and the overall risk is small, with the highest risk in 2019; ranking the risk index shows that the risk rank from 2015 to 2020 is [1, 2, 2, 2, 3, 1]; the hidden risk rank for these six years is [0, 1, 1, 1, 2, 0] obtained by HMM analysis, which is consistent with the observed risk results. And the HMM predicts that the risk level of 2021 is 1, which means that the risk of meat and meat products in Guangzhou develops toward a good trend. Finally, the analysis of the causes of risk differences was conducted, and it was found that there were differences among meat product classifications. The risk indices of salami, chicken and bacon were higher than those of other categories, and the increased detection of salami and bacon in 2019 was a reason for the increased risk. Overall, Guangzhou meat and meat products are less risky, but still need to be monitored for improvement.
    34  Determination of Arsenic Content in High-salt Food Matrices
    DAI Ruiping LIU Huamei SHU Lu TANG Lijun LI Shenying LI Biao ZHANG Meiling LIU Tao HUANG Zonglan
    2024, 40(4):321-330. DOI: 10.13982/j.mfst.1673-9078.2024.4.0479
    [Abstract](66) [HTML](61) [PDF 1.90 M](153)
    Abstract:
    A matrix matching-dynamic reaction cell-inductively coupled plasma mass spectrometry method for determining the arsenic content in high-salt food matrices was established, to solve the mass spectrum interference and matrix interference of a high-salt matrix on arsenic determination. The key technical points for the determination of total arsenic in foods by inductively coupled plasma mass spectrometry and hydride generation atomic fluorescence spectrometry were investigated. A method for determining the arsenic in high-salt food matrices was established through analyzing the effects of pretreatment and digestion methods, organic matrix, chlorine content and internal standard correction elements on the determination of arsenic content. The experimental results show that the microwave digestion method is not quite suitable for the determination of total arsenic in food by hydride generation atomic fluorescence spectrometry. The organic matrix and chlorine will produce a sensitizing effect and mass spectral interference on the mass spectrometry signal of arsenic, respectively. 74Ge is more suitable than 72Ge as an internal standard element for the determination of total arsenic in food by inductively coupled plasma mass spectrometry. LOQ of the established method was 0.010 mg/kg, recovery rate of arsenic in high-salt matrices was 91.50%~110.80% when the addition level was 0.010, 0.025, 0.20 or 0.50 mg/kg, and the relative standard deviation range was 0.76%~7.22%. The statistical values of the sample analysis results obtained by this method and the reference method were both higher than t0.90,10 (1.81, bilateral), and there is a significant difference in the determination results. The measurement results of actual samples revealed that the kinetic energy discrimination measurement model is not suitable for the determination of total arsenic in high-salt food matrix samples. The method is accurate and reliable, and can be applied for the determination of arsenic content in high salt-and high-matrix foods.
    35  Determination of 15 Polycyclic Aromatic Hydrocarbon (PAH) Pollutants in Spices by an Internal Standard High Performance Liquid Chromatography (HPLC)
    HOU Meiling FENG Guirong CAI Xiaoqin HUANG Dandan DONG Xianbing
    2024, 40(4):331-340. DOI: 10.13982/j.mfst.1673-9078.2024.4.0429
    [Abstract](52) [HTML](51) [PDF 4.27 M](151)
    Abstract:
    An internal standard HPLC method was established for simultaneous determination of 15 kinds of PAHs in spices by. The samples were dissolved in sodium hydroxide, extracted with acetonitrile, redissolved by n-hexane, cleaned and concentrated by the Florisil SPE cartridge, and determined by HPLC, with benzo [a] pyrene D12 being used as the internal standard. This method had a good linear relationship in the range of 0~100 ng/mL, with the detection limits of PAHs in Capsicum being 0.5~3.0 μg/kg and the limits of quantitation as 2.0~10.0 μg/kg. The limits of detection and limits of quantitation of PAHs in Zanthoxylum were 0.5~1.0 μg/kg and 2.0~3.0 μg/kg, respectively. When the spiked levels were 2.0, 10.0 and 50.0 μg/kg, the recoveries in the two spices were 60.58%~119.48% and 60.46%~119.24%, with the RSD (n=6) being 1.25%~9.61% and 1.10%~8.56%. The concentration of ΣPAHs in the five spices from high to low were : green Zanthoxylum (561.2 ųg/kg) > erjingtiao (448.8 μg/kg) > red Zanthoxylum (358.3 μg/kg) > shizhuhongi (353.6 μg/kg) > chilli (243.5 μg/kg). The more seriously polluted areas of Chongqing were Bishan, Jiangjin and Rongchang, with ΣPAHs being 721.5, 626.6 and 621.0 μg/kg, respectively. The more seriously polluted compounds in PAHs were phenanthrene, fluoranthrene and pyrene, with their average contents being 215.10, 80.25 and 75.35 μg/kg, respectively. The internal standard method established in this study has high accuracy and good repeatability, which is suitable for the determination of PAHs in spices.
    36  Research Progress on the Efficient Purification of Glucoraphanin
    LI Jinwang ZHEN Shaobo LI Xiude ZHOU Linyi LIU Yiren LI Jinfeng
    2024, 40(4):341-349. DOI: 10.13982/j.mfst.1673-9078.2024.4.0517
    [Abstract](77) [HTML](71) [PDF 4.64 M](172)
    Abstract:
    Glucoraphanin (RAA) is a glucosinolate widely found in cruciferous plants, the metabolites of which have been established to have anti-cancer effects. However, the purity of RAA extracted from cruciferous plants is low, which limits its effective utilization, and consequently, it is necessary to develop an effective process for RAA purification. Although the main technique currently used for the purification of RAA is chromatographic purification, this procedure has notable drawbacks, including low recovery rates and secondary contamination. As an alternative procedure, membrane purification, which is the most commonly used method for the separation and purification of glycosides, requires only simple equipment and entails simple operation. Moreover, it is less prone to secondary contamination. Accordingly, this separation procedure is considered a potential technique for the purification of RAA. It is speculated that combined chromatographic and membrane purification could facilitate the effective recovery of RAA and enhance the purity of the recovered product, thereby overcoming the problems of low recovery and secondary contamination of RAA associated with the sole use of chromatographic purification. This study focuses mainly on the main methods currently used for the purification of RAA and assesses the feasibility of the combined application of chromatography and membrane purification techniques for RAA purification, thereby providing a methodological reference for the efficient purification of RAA.
    37  Research Progress on the Technologies for Regulating Starch Molecular Weight
    ZHANG Sijie ZHANG Xinrui FENG Zhiqiang WANG Zhen WANG Luyang HUANG Jihong
    2024, 40(4):350-362. DOI: 10.13982/j.mfst.1673-9078.2024.4.0307
    [Abstract](71) [HTML](64) [PDF 5.70 M](189)
    Abstract:
    Natural starch is one of the end products synthesized by green plants through photosynthesis, and is one of the main storage polysaccharides in the harvesting organs of crops and one of the key raw materials supporting the development of modern industry. The molecular weight of starch is an important fundamental parameter for studying the molecular structure of starch and is also an important basis for determining the properties and applications of starch. By modulating the molecular weight of starch, many properties of natural starch can be improved, such as thermal stability, gel strength, rheological properties, making the starch more suitable for applications in the pharmaceutical, chemical and food industries, which has become a hot research topic in recent years. This paper reviews summarizes and analyzes the research on the regulation of starch molecular weight from three aspects: physics, chemistry, and biotechnology, and discusses the mechanisms of different regulation techniques and the effects of molecular weight changes on the physicochemical properties and applications of starch. New ideas for regulating starch molecular weight are proposed, and an outlook on future development directions is provided, aiming to provide a theoretical basis for studying starch molecular weight and its applications.

    Editor in chief:李琳

    Inauguration:现代食品科技

    International standard number:ISSN 1673-9078

    Unified domestic issue:CN 44-1620/TS

    Domestic postal code:46-349

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