Volume 40,Issue 12,2024 Table of Contents

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  • 1  Ginsenoside Rg2 Alleviates Lipopolysaccharide-induced Inflammation of RAW264.7 Cells by Regulating the PI3K/AKT/NF-κB Signaling Pathway
    XIE Jing YUAN Qiaoyun HE Ziliang YANG Guigui GAO Xiang ZHANG Wenxia ZHANG Ji HU Weicheng
    2024, 40(12):1-9. DOI: 10.13982/j.mfst.1673-9078.2024.12.0016
    [Abstract](5) [HTML](0) [PDF 4.39 M](4)
    Abstract:
    The anti-inflammatory effects of ginsenoside Rg2 were investigated based on a bacterial lipopolysaccharide (LPS)-induced inflammation model of RAW264.7 cells. The expression levels of inflammation-related genes and proteins in the model were analyzed using PCR, RT-qPCR, and western blotting. The results demonstrated that ginsenoside Rg2 dosedependently inhibited the release of nitric oxide from inflammatory cells (P<0.05) and transcription levels of inflammationrelated genes, including inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor-α, and interleukin-1β (P<0.05). Scanning electron microscopy analysis showed that ginsenoside Rg2 improved the morphology of inflammatory RAW264.7 cells after LPS induction. Western blotting results revealed that ginsenoside Rg2 inhibited the phosphorylation of PI3K, PDK1, AKT, and GSK-3β and prevented NF-κB from entering the nucleus. These results suggest that ginsenoside Rg2 has an anti-inflammatory effect and can reduce LPS-induced inflammation of RAW264.7 cells via the PI3K/AKT/NF-κB signaling pathway. The findings of this study improve the understanding of the anti-inflammatory activity of ginsenoside Rg2 and associated mechanism and provide a foundation for the development of related functional foods.
    2  Regulatory Effects of Enzymatically Converted Chicken Bile on Bile Acid Metabolism and Gut Microbiota in Alcoholic Rats
    JIANG Yanni ZOU Zebin HU Fang LI Yaqi LIN Xucong LI Pan DU Bing QIU Kexian
    2024, 40(12):10-22. DOI: 10.13982/j.mfst.1673-9078.2024.12.1245
    [Abstract](4) [HTML](0) [PDF 12.66 M](3)
    Abstract:
    The regulatory effects of enzymatically converted chicken bile (CB) on bile acid metabolism and gut microbiota dysbiosis in alcoholic rats were investigated. Thirty-five 8-week-old male Sprague Dawley (SD) rats were randomly divided into five groups after 1 week of adaptive feeding: healthy group (HC), model group (MC), low, medium, and high CB dose group (CBL 50 mg/kg, CBM 100 mg/kg, CBH 150 mg/kg). After 1 week of alcohol modeling and CB intervention, the rats were euthanized and serum and cecum contents were collected. The bile acid composition in the serum and the gut microbiota in the cecum contents were analyzed. The results showed that the high-dose CB intervention led to a significant decrease in the concentration of primary bile acids in rat serum, whereas the ratio of unconjugated to conjugated bile acids was reduced by 59.90%, with a significant increase in the level of tauroursodeoxycholic acid (TUDCA), thereby altering the composition of bile acids. CB successfully restored the alcohol-induced reduction in the abundance and diversity of the gut microbiota, resulting in a 53.66% decrease in the ratio of Firmicutes to Bacteroidetes. Furthermore, the abundance of probiotic bacteria such as Parabacteroides and Bacteroides was improved, whereas that of harmful bacteria, such as Romboutsia and Erysipelothrix, was decreased. Meanwhile, the abundance of bile acid-metabolizing bacteria, such as Verrucomicrobiota and Bacteroides, was improved. A strong correlation was found between serum bile acids and gut microbiota. It can be concluded that CB has beneficial effects on the regulation of gut microbiota composition and serum bile acid metabolism.
    3  Antitumor Activity and UPLC-Q-Orbitrap HR/MS Analysis of Allium senescens Extract
    GUO Guangmei WANG Li LI Lilang WANG Yu ZHU Haiyan AN Zhengbin LI Ke SONG Jingrui YANG Xiaosheng LI Qiji
    2024, 40(12):23-31. DOI: 10.13982/j.mfst.1673-9078.2024.12.1237
    [Abstract](4) [HTML](0) [PDF 4.43 M](2)
    Abstract:
    The antitumor activity and chemical composition of extracts from different Allium senescens tissues are presented in this study. MTT assays and scratch tests were used to detect the effects of different plant tissue extracts on the A549 cell proliferation and migration. Cell cycle distribution in control and treated cells was detected by flow cytometry and the expression of cycle-related proteins (CDK2, CDK4, CDC25A, and Cyclin A2) in A549 cells treated with HC-Ea were measured by western blot. The chemical composition of each HC-Ea was analyzed by UPLC-Q-Orbitrap HR/MS. Inhibition of A549 cell proliferation increased with increasing concentration, with respective HC-Ea inhibitory concentrations (IC50 values) of 50.81, 32.09 and 25.02 μg/mL observed at 24, 48, and 72 h, respectively. Compared with DMSO, 50, 100, and 200 μg/mL doses of each HC-Ea reduced the scratch healing rate, with their inhibitory effects intensifying with increasing drug dosage. Following treatment of A549 cells with HC-Ea for 48 or 72 h, the proportion of S phase cells in each concentration group increased significantly in a dose-dependent manner. Compared with DMSO treated cells, the expression of CDK2, CDK4, CDC25A, and Cyclin A2 protein in A549 cells in each HC-Ea treated group was reduced, especially with high concentration exposure. A total of 25 compounds from Allium senescens extract were detected by UPLC-Q-Orbitrap HR/MS, including 10 nitrogen compounds and 8 organic acids. This research on the antitumor activity and chemical composition of Allium senescens provides a new reference for industrial applications.
    4  Skin Barrier Repairing Efficacy and Safety Evaluation of Gastrodia elata Extract
    WANG Weilian ZHANG Ying ZHOU Wen LI Qilin GAO Wenhong LI Li
    2024, 40(12):32-40. DOI: 10.13982/j.mfst.1673-9078.2024.12.1469
    [Abstract](4) [HTML](0) [PDF 2.23 M](2)
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    Gastrodia elata extract (GEE) was prepared using the thermal reflux method and its ability to repair damaged skin barrier was investigated using a HaCaT cell model irradiated with 0.15 J medium-wave ultraviolet B, as well as its moisturizing efficacy was evaluated in humans. At a GEE concentration of 200 μg/mL, the relative cell survival viability was 89.79%, with the inhibition rates of tumor necrosis factor (TNF)-α and prostaglandin E2 (PGE2) being 16.30% and 24.51%, respectively. The reactive oxygen species (ROS) content decreased by 32.70%, thereby upregulating the expression of fillagrin (FLG) and aquaporin 3 (AQP3) mRNA by 65.79% and 189.60%, with the protein contents both increasing by 17.86% and 16.73%, respectively. Moreover, the chick embryo chorionic allantoic membrane assay showed that the GEE samples with a concentration ≤7 mg/mL caused no considerable irritation. Furthermore, the human patch test and moisturizing efficacy evaluation indicated that the gel samples containing 0.5% (mass fraction) GEE caused no adverse reactions on human skin, increased the skin moisture content by 14.28%, and decreased moisture loss by 15.09% compared with that of the blank group. Therefore, GEE exerts barrier repair efficacy by reducing ROS content, inhibiting TNF-α and PGE2 secretion, upregulating AQP3 and FLG mRNA expression, increasing AQP3 and FLG protein content, and improving skin moisture. Owing to its low irritation and high safety, EGG shows promising potential as a soothing and rejuvenating ingredient.
    5  Soluble Expression and Purification of Recombinant Humanized Type I Collagen in Escherichia coli
    TENG Fei KE Bowen QUAN Binghua WANG Yong YI Xiang HAN Shuangyan
    2024, 40(12):41-48. DOI: 10.13982/j.mfst.1673-9078.2024.12.1449
    [Abstract](4) [HTML](0) [PDF 2.54 M](1)
    Abstract:
    Collagen is currently widely used in the food, medical, and beauty industries. However, isolating recombinant collagens poses considerable challenges because of factors such as inclusion body expression and low purity, limiting its large-scale production and application. Based on humanized type I collagen (hCOL1A1), a recombinant expression vector pET21a(+)-SUMO-hCOL1A1 was constructed by fusing a small ubiquitin-like modifier protein (SUMO) and 6×His tag at the N-terminus of hCOL1A1, which was then transformed into Escherichia coli BL21 (DE3). Soluble expression and purification of recombinant hCOL1A1 in E. coli were achieved through IPTG induction, optimization of the culture conditions, and protein purification of the engineered strain produced by BL21/pET21a(+)-SUMO-hCOL1A1. The supernatant of the engineered strain was purified through Ni-NTA affinity chromatography, TEV enzyme digestion, and ionexchange chromatography, ultimately yielding hCOL1A1 protein with a purity of over 95%. The optimal culture conditions were determined to be an induction temperature of 25 ℃ and a concentration of 0.4 mmol/L IPTG. Further scale-up in 5 L fed-batch fermentation resulted in a final protein yield of 1.43 g/L. Soluble expression of the hCOL1A1 protein, 5 L bioreactor production, and purification were achieved, serving as a valuable reference for the large-scale biosynthesis and purification of recombinant collagen.
    6  Optimization of Enzymatic Preparation Process of Antioxidant Peptides from Prinsepia utilis Royle and Analysis of Their in Vitro Antioxidant Activities
    WANG Zengli FU Jian HE Tong WANG Xuefeng FAN Jiangping HUANG Aixiang
    2024, 40(12):49-56. DOI: 10.13982/j.mfst.1673-9078.2024.12.1215
    [Abstract](5) [HTML](0) [PDF 4.91 M](3)
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    Prinsepia utilis Royle is a food and medicine homologous woody oil crop with Yunnan characteristics. The by-products after oil extraction have the problem of low protein utilization. In this experiment, Prinsepia utilis Royle meal was used as the raw material to produce protein hydrolysate with antioxidant activity. The single factor experiments and response surface optimization analysis were carried out on three factors, enzyme mass fraction, enzymatic hydrolysis time and solid-liquid ratio, to determine the enzymatic hydrolysis process conditions for the preparation of protease hydrolysate from Prinsepia utilis Royle meal by alkaline protease. The results showed that the optimal enzymatic hydrolysis conditions were: enzyme mass fraction, 4.6%; enzymatic hydrolysis time, 4.6 h; solid-liquid ratio, 1:31 g/mL. Under the optimal conditions, when the mass concentration of antioxidant peptide prepared by enzymatic hydrolysis was 0.9 mg/mL, the scavenging capacities for DPPH free radical, ABTS+ free radical and hydroxyl free radical were 76.46%, 94.22% and 73.02%, respectively. In addition, the results of cytotoxicity experiments showed that the peptide had no toxic effect on the growth of HepG 2 cells. Taken together, the antioxidant peptide prepared through this process had good antioxidant activity. This study can provide a reference for the development and utilization of Prinsepia utilis Royle protein resource.
    7  Mining the Geraniol Dehydrogenase Gene in a Citral Chemotype of Elsholtzia cyprianii via High-throughput Transcriptome Sequencing
    XU Yuqi LIANG Chunmao CHENG Jiahui LI Yuanyuan PU Chunxia
    2024, 40(12):57-66. DOI: 10.13982/j.mfst.1673-9078.2024.12.1390
    [Abstract](4) [HTML](0) [PDF 5.70 M](2)
    Abstract:
    The essential oil of a medicinal and edible citral chemotype of Elsholtzia cyprianii was extracted and it components were subsequently identified using GC-MS. Transcriptome data were obtained using Illumina high-throughput sequencing (NovaSeq 6000), and key enzyme-coding genes involved in essential oil synthesis were identified. The results showed that the citral content of the E. cyprianii germplasm exceeded 80%. After high-throughput sequencing, a total of 22.08 GB of data and 147 186 998 clean reads were obtained. In total, 33 720 unigenes with an average length of 1799 bp were generated using the Trinity assembly, and all were successfully functionally annotated in seven major open databases. KEGG metabolic pathway analysis revealed that the unigenes were enriched in two monoterpene pathways, with 109 unigenes related to terpenoid backbone biosynthesis (No. ko00900) and 32 unigenes associated with monoterpenoid biosynthesis (No. ko00902). The 1143 bp geraniol dehydrogenase (GeDH) gene encoding 380 amino acids was amplified. The GeDH protein contained conserved Zn2- and NADP-binding sites and a Zn1 structural group similar to that in Perilla. Our findings provide a new breakthrough point for further research on the biosynthesis pathway of monoterpenoids in the characteristic essential oils of Elsholtzia. The mining of GeDH, which encodes a key enzyme that regulates the synthesis of citral essential oils, will prove invaluable in the development and utilization of natural citral essential oils and lays the foundation for increasing the variety of edible flavored oils and natural antibacterial agents for use in food products.
    8  Intracellular Expression, Isolation and Purification of LfcinB and EGFP Fusion Protein in Pichia pastoris
    LIU Wei WANG Yulian WANG Lin WU Haodong TIAN Huimin CHENG De WANG Liang
    2024, 40(12):67-74. DOI: 10.13982/j.mfst.1673-9078.2024.12.1223
    [Abstract](4) [HTML](0) [PDF 4.61 M](3)
    Abstract:
    In this study, the enhanced green fluorescent protein (EGFP) gene fragment was ligated with the N-terminus of the antimicrobial peptide bovine lactoferrin peptide (LfcinB-His) gene fragment to construct an intracellular expression vector with EGFP as the reporter gene. The recombinant protein EGFP-LfcinB-His was fused and expressed, and its activity was determined. The recombinant expression plasmid was electroporated into Pichia pastoris GS115, and the positive transformants were screened. The fermentation expression was induced by methanol, and strong fluorescence was detected under the excitation light of 488 nm. When the yeast cell culture reached the highest density, the cells were collected, the cell walls were broken, and the cells were lysed with a yeast cell protein lysate. The target protein (LfcinB-His) band with a relative molecular mass of 4.1 ku was detected by Tricine-SDS-PAGE, after the lysate was purified by steps such as ultrafiltration concentration, Ni-NTA affinity chromatography and formic acid lysis. Finally, a LfcinB-His ultrafiltration concentrate with a purity of 90.32% was obtained through the determination and analysis by liquid chromatography-mass spectrometry, which had different degrees of inhibition on the six strains of pathogenic bacteria tested, and the inhibitory concentration range was 16~64 μg/mL. In summary, this study provides a good methodological reference for the expression of small molecular peptides in Pichia pastoris, and also lays a foundation for further research on the biological activity and high-density fermentation of LfcinB.
    9  Analysis of Microbial Composition during the Germination of Bean Sprouts
    ZHANG Haoqi LIU Yushan WU Chuqian ZHENG Jiayi WU Xiyang
    2024, 40(12):75-81. DOI: 10.13982/j.mfst.1673-9078.2024.12.1345
    [Abstract](4) [HTML](0) [PDF 1.78 M](3)
    Abstract:
    The microbial growth and identification of main microorganisms in mung bean sprouts, soybean sprouts, pea seedlings sold in Guangzhou (supermarkets and farmers' markets), and the laboratory-grown mung bean sprouts were studied, and the overall microecological composition of bean sprouts was determined using 16S gene high-throughput sequencing method. The results showed that the total number of microorganisms in the finished mung bean sprouts was 106~107 CFU/g, which mainly came from the bean seeds (8.80×107 CFU/g in the cotyledon). The total number of the microorganisms was 8.10×106 CFU/g for the sterilized group after 36 h of germination and 7.90×106 CFU/g for the unsterilized group after 24 h of germination. The major microbial species were mainly Klebsiella, Enterobacteria, Pantobacteria, and Citrobacter. The core microbial groups in the overall microecology were Cyanobacteria, Proteobacteria, Firmicutes, and Bacteroidetes. Eleven drug susceptibility tests were conducted on 20 strains of Enterobacteriaceaes isolated from mung bean sprouts by the disk diffusion method. The results showed that the bacteria exhibited significant resistance to five antibiotics, namely erythromycin, rifampicin, cefoxitin, clindamycin, and penicillin. Results of this study showed that the commercially available bean sprouts carried a large number of microorganisms, among which the Enterobacteriaceae are opportunistic pathogens with broad-spectrum antibiotic resistance. Chemical disinfectants could extend the shelf life of mung bean sprouts by about 12 h. Consuming raw or inappropriately cooked bean sprouts would cause safety problems, which is worthy of reference for manufacturers, government regulatory authorities and consumers.
    10  Optimization of the Fermentation Process Using Aspergillus niger to Prepare Proanthocyanidins from Litchi Husks and Their Effects on the Proliferation and Migration of HaCaT Cells
    DONG Lihong ZHENG Deyu ZHANG Mingwei ZHANG Ruifen HUANG Fei MA Qin ZHAO Dong GUO Chaowan JIA Xuchao
    2024, 40(12):82-90. DOI: 10.13982/j.mfst.1673-9078.2024.12.1519
    [Abstract](4) [HTML](0) [PDF 2.95 M](2)
    Abstract:
    Aspergillus niger was used to ferment litchi husk, and the effects of fermentation time, inoculum amount and solid-to-solvent ratio on the yield of proanthocyanidins (LPPC) from litchi husk by were investigated, and the process conditions for the fermentation of litchi husk were optimized. The changes in the contents of proanthocyanidins, total polyphenols and total flavonoids and the composition and content of monomeric phenolics in litchi husk before and after fermentation were analyzed. The antioxidant activity of LPPC before and after fermentation and the effect of LPPC on the proliferation and migration of HaCaT cells were evaluated. The optimum process conditions determined through single factor experiments combined with the orthogonal test were: fermentation time, 7 days; inoculum amount, 2% (V/V); solidto-solvent ratio 1:10 (g/mL). The yield of LPPC prepared under such process conditions was 3.77% (m/m). Compared with those of the unfermented sample, the contents of procyanidins, total polyphenols and total flavonoids of the fermented litchi husk increased by 112.75%, 53.86% and 73.02%, respectively, the contents of the seven main monomeric phenolics increased significantly, and the antioxidant activity of LPPC as ABTS scavenging capacity and FRAP significantly increased after fermentation. Before and after fermentation at a mass concentration of 10, 20 or 40 μg/mL, LPPC had no effect on the proliferation of HaCaT cells, but significantly promoted cell migration, with the cell migration rate for the LPPC after fermentation increasing by 7.89%, 40.89% and 75.37%, respectively, compared with that for the unfermented sample. Therefore, the fermentation with Aspergillus niger can significantly increase the content of proanthocyanidins from litchi husk, thereby increasing the antioxidant capacity and promoting wound healing by keratinocytes. The research results will provide a theoretical basis for the application of LPPC in epidermal repair after skin injury.
    11  Application and Evaluation of the PepOSX Workstation in Peptidomic Analysis of Food-derived Protein Hydrolysates
    LIU Wanshun ZHENG Lin LI Wenzhi LIU Lei CEN Xingyi CHEN Tiange ZHOU Zihang XU Jucai ZHAO mouming
    2024, 40(12):91-97. DOI: 10.13982/j.mfst.1673-9078.2024.12.1490
    [Abstract](3) [HTML](0) [PDF 7.79 M](3)
    Abstract:
    Peptidomic analysis is the key to elucidating the changing trend of peptides during processing. In this paper, the performance of the PepOSX software in peptidomic identification of the peptides in food-derived protein hydrolysates spiked with known peptide standards was evaluated, and the effects of different identification approaches and working parameters on the detection rates and accuracy of single peptides were studied. For the sequence database-independent analysis of the known collagen and casein peptide standard mixtures, the accuracy rates of the Exhaust engine were 67.86% and 36.36%, respectively, and the corresponding detection rates reached 80% and 100%, respectively, indicating the good adaptivity of the engine for discovering unknown short peptides. The accuracy rates of the Search engine based on sequence database for the identification of the above-mentioned mixed standards were as high as 71.43% and 90.48%, respectively, with the corresponding detection rates being 91.30% and 100%, showing the superior capabilities of the engine in analyzing peptides from the samples with known protein sources. The above-mentioned engines were combined and applied in peptidomic analysis of protein hydrolysates. Compared with the performance of SEQUEST, MyriMatch and X! Tandem, PepOSX has a much higher accuracy rate, and the number of identified peptides rom the casein and soybean protein hydrolysates was about 3~4 times that of the latter. This fully demonstrated the applicability and reliability of the PepOSX workstation in the identification of non-specific peptides. In the future, PepOSX is expected to provide effective technical support for exploring new bio-active peptides or revealing the mechanism underlying the changes in peptides.
    12  Identification of Lactobacillus apis A9 Isolated from Honey and Quantitation of its Lead Adsorption
    Shen Wenquan Gao Xibei Hu Hao Qiao Jiangtao Zhang Hongcheng
    2024, 40(12):98-109. DOI: 10.13982/j.mfst.1673-9078.2024.12.1429
    [Abstract](4) [HTML](0) [PDF 3.88 M](3)
    Abstract:
    In an investigation of the probiotic properties of lactic acid bacteria in honey, a gram-positive bacterium was isolated from honey and purified. Based on its colony and cell morphology, physiological and biochemical responses, and 16S rDNA sequencing analysis, the bacterium was identified as Lactobacillus apis A9. This strain was found to be negative for hemolysis, gelatin hydrolysis, nitrate reduction, and amino acid decarboxylation, and susceptible to nine antibiotics, including penicillin. In probiotic experiments the bacterium displayed a high self-aggregation rate (93.37%) and a relatively high hydrophobicity rate (48.71%). It exhibited 43.67% survival after incubation for 3 h at pH 3.0. After 24 h of incubation in solutions of 1 wt.% sodium deoxycholate, 6 wt.% sodium chloride, and 25 wt.% glucose, the survival rates of the bacterium were 96.48%, 68.27%, and 88.57%, respectively. These results indicate that strain A9 possesses strong resistance to acid, bile salts, and hyperosmotic stress. The bacterium displayed a lead (Pb2+) absorption rate of 55.53%. Lead accounted for 5.90% of the bacterium’s surface-displayed elements. Infrared results revealed that hydroxyl and amino groups on the bacterium’s surface interact with lead ions, leading to an increase in α-helices and β-turns in the bacterium’s proteins and a decrease in β-sheets. In summary, L. apis A9 isolated from honey exhibits good safety, probiotic function, and lead adsorption capacity. This study provides new insights into the development and utilization of lactobacilli in honey.
    13  Optimization of Fermentation Conditions for Antimicrobial Substance Production from Paenibacillus thiaminolyticus SY20 using Response Surface Methodology
    WU Yaping CAO Cai LIN Yongwen CHEN Jianxuan CHEN Jiankai LIN Liangmei YE Huilian LIU Dongmei
    2024, 40(12):110-118. DOI: 10.13982/j.mfst.1673-9078.2024.12.1382
    [Abstract](5) [HTML](0) [PDF 4.74 M](2)
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    Paenibacillus thiaminolyticus SY20 is an antimicrobial strain isolated from acid bamboo shoots in Zhangzhou, Fujian. Previous studies have demonstrated that this species can produce a rare lipopeptide antimicrobial substance, polymyxin A1. To improve the ability of P. thiaminolyticus to produce antimicrobial substances, the active strain was used as the research object, and the antimicrobial diameters against Escherichia coli were used as the investigation index to optimize the fermentation conditions. Single-factor experiments were conducted to evaluate the effects of different carbon sources, nitrogen sources, and inorganic salt types and their concentrations on the production of antimicrobial substances. The three significant factors, namely ammonium phosphate, KCl, and NaCl, were screened and further analyzed using the Plackett-Burman method. The optimal medium combination was determined using a Box–Behnken central composite design. Finally, the inoculation volume, pH value, fermentation time and temperature were optimized to obtain the most effective fermentation conditions, which were then verified experimentally. The results showed that the optimum fermentation medium for producing antimicrobial substances in P. thiaminolyticus SY20 was composed of 20 g/L maltose, 15 g/L cottonseed cake flour, 8 g/L (NH4)2HPO4, 10 g/L KCl, and 10 g/L NaCl. The optimized fermentation conditions were as follows: pH 7.5, fermentation temperature of 28 ℃ , and fermentation time of 60 h. After optimization, the inhibition diameter of the fermented supernatant was 22.8 mm, which was 1.5-fold larger than that before optimization (15.2 mm). The ability of the strain to produce antimicrobial substances was significantly improved, providing a foundation for large-scale isolation and purification of the antibacterial substances.
    14  Prevention and Control of Biofungicides on Four Postharvest Pathogenic Fungal Strains in Dragon Fruit
    LUO Donglan WANG Xiaogang QU Guangfan ZHAO Zhibing BA Liangjie
    2024, 40(12):119-133. DOI: 10.13982/j.mfst.1673-9078.2024.12.1324
    [Abstract](4) [HTML](0) [PDF 4.18 M](2)
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    To clarify the prevention and control effect of five biofungicides (matrine, zhongshengmycin, kasugamycin, polymyxin, and validamycin) on postharvest pathogenic fungi of dragon fruit (Hylocereus undatus), this study aimed to determine the mycelial growth inhibition, laboratory virulence, mycelial conductivity, and nucleic acid leakage of four postharvest pathogenic fungal strains in dragon fruit (Nigrospora sphaerica, Fusarium oxysporum, Epicoccum nigrum, and Neoscytalidium dimidiatum) after treatment with the five biofungicides. In addition, propidium iodide (PI) staining test was conducted to investigate the damaging effect of biofungicides on the cell membranes of pathogenic fungi. The results showed that the inhibition rate of zhongshengmycin on the mycelial growth of E. nigrum was 100% at 3.00 g/L, and the corresponding concentration for 50% of maximal effect (EC50) was 0.59 g/L. The inhibition rates of Picrasidine on the mycelial growth of F. oxysporum, N. sphaerica, and N. dimidiatum were 90.93, 84.17, and 87.80%, respectively, and the corresponding EC50 values were 0.94, 0.71 and 0.62 g/L, respectively. The PI staining test showed that the five biofungicides damaged the cell membranes of the mycelia of the pathogenic fungi in the postharvest H. undatus. Zhongshengmycin and Picrasidine had good inhibitory effects, making them adequate biofungicides for postharvest pathogenic fungi of dragon fruit. This study provides a theoretical basis for the prevention and control of postharvest diseases of H. undatus and the screening of biofungicides applicable to field production.
    15  Study on the Ripening Characteristics and Storage Quality Changes of Camembert Cheese
    WAN Jun ZHANG Xiaoqin WANG Ziyuan AI Minmin XIE Zhequan
    2024, 40(12):134-142. DOI: 10.13982/j.mfst.1673-9078.2024.12.1369
    [Abstract](4) [HTML](0) [PDF 2.64 M](2)
    Abstract:
    The changes in the quality characteristics of Camembert cheese during storage were investigated to determine its optimal maturity time. Camembert cheese was selected as the research subject, and the storage conditions were set to 12 ℃ and 95% relative humidity. The physicochemical properties, flavor changes, and textural characteristics at different ripening stages (0, 5, 10, 15, 20, and 25 d) were measured. The results revealed that the moisture content, pH value, and free amino acid content of the cheese significantly increased with the increase in fermentation time (P<0.05). These changes stabilized after 20 days, with the moisture content at 61.34%, pH value at 6.68, and free amino acid content at 0.24%. Simultaneously, the protein content gradually decreased, while the soluble nitrogen/total nitrogen ratio significantly increased (P<0.05). The pH value 4.6 soluble nitrogen/total nitrogen ratio and its increasing rate from 0.06% on 0 d to 0.49% on 25 d were particularly notable. GC-MS analysis revealed that despite no significant differences in the types of fatty acids, the relative content of long-chain fatty acids (such as palmitic acid and oleic acid) was higher and more dominant. In contrast, the content of caproic acid, a short-chain fatty acid, decreased from 1.33% to 0.98%. Furthermore, it was observed that the textural characteristics of Camembert cheese was optimal on the 20th day of storage, with its hardness stabilizing at 3230.17 g and springiness reaching a maximum of 0.83. Therefore, the optimal ripening time for Camembert cheese was determined to be 20 days, at which its physicochemical properties, flavor changes, and textural characteristics reached optimal levels. The findings of this study may provide theoretical support for storage quality control of Camembert cheese.
    16  Changes in Muscle Quality and Microbial Community Structure of Eel Slices during Cold Storage
    Yang Lifeng Li Xuhai Wang Lan Zhou Zhi Wu Wenjin Li Ping Xiong Guangquan Shi Liu
    2024, 40(12):143-153. DOI: 10.13982/j.mfst.1673-9078.2024.12.1277
    [Abstract](3) [HTML](0) [PDF 4.60 M](2)
    Abstract:
    The changes in muscle quality and microbial community structure of eel (Asian swamp eel, Monopterus albus) slices during 7 days of cold storage at 4 ℃ were assessed by measuring physicochemical indexes, such as color difference, microstructure, pH value, total volatile base nitrogen (TVB-N), and total viable count (TVC), as well as flavor-related substances, such as volatile compounds and free amino acids. Furthermore, associations of bacterial genera with characteristic chemical indexes, color difference indexes, and key amino acids were examined. The results showed, that with the prolongation of cold storage, the color of eel slices gradually became yellow and finally turned brown, and the muscle cell gap, TVB-N content, TVC, and H* value all gradually increased, whereas the R630/580 ratio and the levels of essential amino acids gradually decreased. Specifically, the pH value diminished from 7.02 to 6.19 on day 1 (P<0.05), while the TVC and TVB-N contents exceeded the corresponding limits on days 3 and 7, respectively. The response of eel slices to the sensor W5S was the highest during the cold storage period. After day 3, the odors of the eel slices changed considerably, and the leucine contents decreased. The microbial diversity of eel slices gradually decreased during cold storage, and the dominant genera were Macrococcus and Acinetobacter in the early cold storage period (first three days) and Brochothrix, Pseudomonas, and Carnobacterium after day 3. The results of correlation analysis showed that R630/580, H*, pH, TVC, and TVB-N values as well as the serine, glycine, and leucine contents significantly correlated with the microbial community structure during cold storage; furthermore, microbial growth was the main reason for the changes in freshness, color, and flavor of eel slices during cold storage. This study provides a theoretical basis for cold processing of Asian swamp eels.
    17  Changes in Storage Quality and Endogenous Hormones of Pitaya after the Treatment with Gibberellin-chitosan Combination
    CHEN Lan GU Hui LI Ya ZHOU Kaibing DU Liqing
    2024, 40(12):154-161. DOI: 10.13982/j.mfst.1673-9078.2024.12.1451
    [Abstract](4) [HTML](0) [PDF 4.65 M](1)
    Abstract:
    The preservation effect of the treatment with gibberellin-chitosan combination on postharvest pitaya and the changes in endogenous hormones were investigated in this study. 'Jindu No.1' pitaya was used as the test material, and the storage quality and endogenous hormone content of postharvest pitaya were regularly measured after the treatment with the combination. The results showed that the preservation effect of the treatment with 50 mg/L gibberellin+chitosan was the best. After 12 days of storage, compared with the control, the decay index, bract yellowing rate, and weight loss rate of the treated pitaya were reduced by 55.56%, 15.91%, and 20.75%, respectively, the contents of its total soluble solids (TSS), betaine and vitamin C (Vc) increased by 17.76%, 24.70%, and 17.85%, respectively, and its total antioxidant capacity (T-AOC) and total phenolic content also increased. After 9 days of storage, compared with the control, the endogenous hormone content of the treated pitaya changed the most, with its abscisic acid (ABA) content decreasing by 1 436.1 ng/g, and its contents of gibberellin (GA4) and auxin (IAA) increasing by 0.29 ng/g and 192.3 ng/g, respectively. It can be seen that the treatment with 50 mg/L gibberellin+chitosan can maintain the metabolic balance of endogenous hormones in pitaya, improve the antioxidant capacity of pitaya and delay the decline of storage quality, ripening and senescence of pitaya. Thus, the treatment with the combination can be used as a green and environmentally friendly approach for postharvest storage and preservation of pitaya.
    18  Effect of Applying a Novel Modified Film for Modified Atmosphere Packaging on Strawberry Storage
    Bao Hongyan Dong Tungalag Song jing Yun Xueyan EERDUNBAYAER
    2024, 40(12):162-169. DOI: 10.13982/j.mfst.1673-9078.2024.12.1118
    [Abstract](3) [HTML](0) [PDF 2.10 M](3)
    Abstract:
    For better storage and transportation of strawberries, polyethylene (PE) was modified with poly(butylene adipate-co-terephthalate) (PBAT) and poly(butylene succinate) (PBS) to produce a novel PE/PBAT/PBS blend film with improved gas permeability and antifogging ability suitable for modified atmosphere packaging of strawberries. The PE/PBAT/PBS blend film was prepared by a twin-screw extrusion line and compared with a commercial PE film by analyzing the physical and chemical indexes of strawberries preserved in bags made of both films. The effect of applying the PE/PBAT/PBS blend film for spontaneous modified atmosphere packaging on strawberry preservation was evaluated by measuring the gas concentrations, sensory scores, weight loss rate, soluble solids content, and total phenolic and flavonoid contents of strawberries. At the third day of storage, the CO2 and O2 concentrations in the PE/PBAT/PBS packaging bags were consistent between 5%~7% and 3%~5%, respectively. Under these conditions, the favorable sensory quality of strawberries was maintained for 23 days, with a weight loss below a mass fraction of 5%; on day 23 of storage, the vitamin C content, firmness, and total soluble sugar of the strawberries only decreased to 44.72 mg/100 g, 2.83 kg/cm2, and 8.47%, respectively, with a color difference of approximately ten and consistently high flavonoid and total phenolic contents. These findings demonstrate that the PE/PBAT/PBS blend film suppresses the respiration of strawberries, providing theoretical support for its ability to extend strawberry shelf life.
    19  Effect of Different Heat Treatments on the Structure and Processing Properties of Buffalo Milk Casein
    Yang Pan Li Ling Huang Li Dan Xia Huang Zizhen Zeng Qingkun
    2024, 40(12):170-177. DOI: 10.13982/j.mfst.1673-9078.2024.12.1192
    [Abstract](3) [HTML](0) [PDF 2.45 M](3)
    Abstract:
    The non-heat-treated buffalo milk casein was used as a control to investigate the effects of four common heat treatments on the structure of buffalo milk casein. The results showed that macromolecular polymers were produced by heating and were mainly formed by proteins linked by disulfide bonds. After the heat treatment of 80 ℃, buffalo milk casein had the highest foaming capacity (FC) and foaming stability (FS) at 137.33% and 32.33%, respectively. After ultra-high temperature sterilization (135 ℃), the buffalo milk casein enlarged significantly to 516.2 nm, with destroyed casein micelles and an altered surface structure. Heat treatments modified the secondary structure of casein; notably, at 135 ℃, the α-helix and β-sheet contents decreased significantly, with minimum values of 13.76% and 35.84%, respectively. Heat treatments also altered the tertiary structure and surface hydrophobicity of casein; the pasteurized milk (80 ℃) had the highest fluorescence intensity at 582.44, and the ultrahigh-temperature sterilized milk had the highest surface hydrophobicity at 258.96. These results indicated that pasteurization has a relatively small effect on casein, while the ultra-high temperature condition tends to cause protein aggregation, affecting product stability. Therefore, 80 ℃ is an optimal temperature for the heat treatment of buffalo milk. The findings of this study provide a theoretical basis for improving the stability of buffalo dairy products in the production process.
    20  Effects of Ultrasonic Treatment and Direct Current Magnetic Field on Physicochemical and Gel Properties of Spent Hen Breast Batter
    He Xinyan Hu Li Li Chunqiang Zhang Yanwei Sun Weiqing Shao Junhua
    2024, 40(12):178-187. DOI: 10.13982/j.mfst.1673-9078.2024.12.1445
    [Abstract](3) [HTML](0) [PDF 3.84 M](4)
    Abstract:
    The physicochemical and gel properties of ultrasound- and direct current magnetic field-treated spent laying hen breast batter at phase transition temperatures (76 ℃ and 84 ℃) were analyzed. The results showed that both ultrasound and direct current magnetic field enhanced the thermal processing properties of spent laying hen breast batter. At 84 ℃, both ultrasonic treatment and magnetization resulted in reduced firmness, increased elasticity, and improved water retention properties of batter gels. Notably, ultrasonic treatment significantly altered the moisture content of batter gels, whereas magnetization yielded a moisture content closer to that of the control group. At 76 ℃, ultrasonic treatment led to improvements in the experimental group, but the measured indices changed more obviously after 15 min magnetization (firmness reduced to 627.00 g, elasticity increased to 3.60 mm, cooking loss decreased to 12.47%, pH value decreased significantly to 7.39, and content of flow-resistant water changed to 60.02%), which significantly (P<0.05) improved the gel properties of spent laying hen breast batter to values closer to those of commercial broilers. These results indicate that compared with ultrasonic treatment, magnetization for 15 min can improve the thermal processing properties of spent laying hen breast batter. As magnetization has a short operation time and is convenient and simple, it can improve the production and processing of spent laying hens. These findings provide technical support for the factory production of spent laying hen breast batter gels.
    21  Relationship between Emulsifying and Interfacial Properties of Nonionic Emulsifiers and Their Application to Gluten-free Cakes
    WU Mingqiao Qin Weimei Fan Renyu YI Huanghui Wu Leiyan
    2024, 40(12):188-200. DOI: 10.13982/j.mfst.1673-9078.2024.12.1626
    [Abstract](3) [HTML](0) [PDF 17.51 M](3)
    Abstract:
    In order to use nonionic emulsifiers to improve the poor structure of gluten-free cakes and other problems. In this paper, the properties of interfacial mechanics, kinetics and rheology of four nonionic emulsifiers, namely, Tween 80 (polyoxyethylene sorbitan monooleate, TW80/T), sucrose fatty acid esters (SE/S), propylene glycol fatty acid esters (PGMS/P), and lactic and fatty acid esters of glycerol (LACTEM/L), both singly and after compounding were investigated, to determine the correlation between the interfacial properties and the emulsification activity and stability, and meanwhile, the effect of the compounded emulsifiers on the quality of gluten-free cake was investigated. The results showed that the interfacial pressures of single emulsifiers, except SE, reached the maximum value (13 mN/m) at 0.1% mass concentration, and the emulsification activity was best at 0.1% mass concentration (75.46~109 m2/g). The composite systems SPL+0.01% T, TPL+0.1% S, TSL+0.1% P, and TSP+0.3% L (mass fraction) reached the maximum interfacial composite expansion modulus at the oil-water interface (8.65, 8.31, 6.65, and 11.69 mN/m), and the composite emulsifiers had the best emulsification stability under this condition (83.57%~99.87%), the interfacial composite swelling modulus was positively correlated with the emulsification stability (P<0.01). The addition of the complex emulsifier increased the specific volume of the cake by 1.13~1.66 mL/g, the elasticity increased significantly to 0.97~0.99, and the hardness decreased by 89.66 g. Therefore, the complex emulsifier can improve the quality of the glutenfree cake, and a theoretical basis for the development of the application of the complex emulsifier is provided in this study.
    22  Optimization of Lanzhou Lily Polysaccharide-Fe (III) Complex Preparation using Box-behnken Design and Evaluation of its in Vitro Antioxidant Activity
    LI Xiaojun MA Xiaohui DUAN Guojian JIANG Hong ZENG Fankui GAO Zuowang DONG Wenjing WANG Yinquan JIN Ling
    2024, 40(12):201-208. DOI: 10.13982/j.mfst.1673-9078.2024.12.1550
    [Abstract](3) [HTML](0) [PDF 2.31 M](3)
    Abstract:
    Lanzhou lily polysaccharides and ferric chloride were used as raw materials to prepare the Lanzhou lily polysaccharide-Fe (III) complex. The preparation conditions were optimized using the Fe3+ content as the evaluation index based on a single-factor experimental design and Box-Behnken response surface methodology. The structure of the polysaccharide-Fe(III) complex was analyzed using infrared spectroscopy and ultraviolet-visible spectroscopy, and its in vitro antioxidant activity was evaluated based on the DPPH and ABTS+ radical scavenging abilities. The results showed that the optimal parameters for the preparing Lanzhou lily polysaccharide-Fe(III) complex were as follows: reaction temperature of 61.10 ℃, pH of 6.92, and reaction time of 1.38 h. The Fe3+ content of the complex was 7.32% under these reaction conditions. The results of infrared and ultraviolet analyses indicated that Lanzhou lily polysaccharides chelated with Fe3+. Furthermore, the half-maximal inhibitory concentrations of the Lanzhou lily polysaccharide-Fe(III) complex and Lanzhou lily polysaccharides against DPPH radicals were 0.63 and 0.79 mg/mL, respectively. The corresponding half-maximal inhibitory concentrations against ABTS+ radicals were 0.71 and 0.76 mg/mL, respectively. Additionally, the antioxidant activity of the complex was considerably stronger than that of lily polysaccharides. The Lanzhou lily polysaccharide-Fe(III) complex with antioxidant activity was successfully prepared. The findings of this study provide a theoretical foundation for maximizing the use of Lanzhou lily resources and creating new health products.
    23  Effects of Ultrafine Grinding on the Composition and Activity of Black Rice Bran Polyphenols
    HU Ting WANG Xin GENG Kezan MA Qin
    2024, 40(12):209-217. DOI: 10.13982/j.mfst.1673-9078.2024.12.0068
    [Abstract](3) [HTML](0) [PDF 2.04 M](3)
    Abstract:
    To investigate the effects of ultrafine grinding on the composition as well as the in vitro antioxidant and α-glucosidase inhibition activities of black rice bran polyphenols, ultrafine grinding was performed to prepare ultrafine black rice bran powder. The compositions, anthocyanin contents, total antioxidant capacities, oxygen radical absorption capacities, peroxide radical scavenging abilities, and α-glucosidase inhibitory abilities of polyphenols of ultrafine and coarse black rice bran powder were determined. The results showed that the particle size of black rice bran was significantly reduced by ultrafine grinding. Compared with those of the coarse powder, the extraction yields of free polyphenols, total polyphenols, and anthocyanins of ultrafine black rice bran powder were increased by 8.27%, 10.72%, and 21.97%, respectively. Furthermore, the extraction yields of phenolic monomers, such as ferulic acid, p-coumaric acid, and vanillic acid, were significantly increased, with no changes in the phenolic monomer composition. Polyphenols of the ultrafine powder exhibited higher antioxidant and α-glucosidase inhibitory abilities than those of the coarse powder. Particularly, free polyphenols were the main contributors to these activities. In conclusion, ultrafine grinding promotes the release of polyphenols and enhances the antioxidant capacity and α-glucosidase inhibitory abilities of black rice bran. This pretreatment method can be applied in the processing and utilization of black rice bran.
    24  Gelatinization and in Vitro Digestive Properties of Lotus Root Whole Powder Prepared using Various Processing Techniques
    LI Yifan WU Jinjin YI Yang PENG Kaidi SUN Ying ZHU Rui
    2024, 40(12):218-226. DOI: 10.13982/j.mfst.1673-9078.2024.12.1388
    [Abstract](3) [HTML](0) [PDF 3.36 M](2)
    Abstract:
    Lotus root whole powder (LRWP) were prepared from the fresh roots of Wuzhi Ⅱ lotus using several processing techniques and/or freeze-drying. The processing techniques included tea polyphenol addition (i.e., adding tea polyphenols to lotus root pulp) (Ⅰ), gelatinization and retrogradation (i.e., high-temperature gelatinization and retrogradation of lotus root pulp) (Ⅱ), tea polyphenol addition-gelatinization and retrogradation (Ⅰ-Ⅱ), gelatinization-pullulanase debranching-retrogradation (Ⅱ-Ⅲ), and gelatinization-pullulanase debranching-tea polyphenol addition-retrogradation (Ⅰ-Ⅱ-Ⅲ). LRWP samples prepared via these processing techniques were denoted as LRWP-Ⅰ, LRWP-Ⅱ, LRWP-Ⅰ-Ⅱ, LRWP-Ⅱ-Ⅲ, and LRWP-Ⅰ-Ⅱ-Ⅲ, respectively, and the LRWP samples prepared by direct freeze-drying were used as the control (denoted as LRWP). The impact of different processing techniques on the gelatinization and digestive properties of LRWP samples was analyzed. The results showed that LRWP-I had the highest peak viscosity (4 596 cP) and breakdown viscosity (3 322 cP) among groups, whereas the final viscosity, setback viscosity, and gel properties of LRWP-Ⅰ were lower than those of LRWP. The firmness of LRWP-II (89.7 g) and LRWP-Ⅰ-Ⅱ (93.1 g) was significantly higher than that of LRWP (37.6 g). LRWP-II-III had the lowest peak viscosity of 43 cP and the worst gel properties. LRWP-Ⅰ-Ⅱ-Ⅲ had a lower gelatinization temperature, viscosity, and gel properties compared with those of LRWP. Additionally, in vitro digestion experiments indicated that the content of resistant starch in LRWP-Ⅰ (92.65%) was significantly (P<0.05) higher than that in LRWP (77.88%). In conclusion, applying tea polyphenol addition (I) alone increased the content of resistant starch, and the corresponding product LRWP-I showed good gelatinization and digestive properties. This study provide theoretical guidance for the development and application of LRWP as well as the preparation of resistant starch.
    25  Process Optimization for Softening of Crayfish (Procambarus clarkii) Shells
    QIN Jingkai CHEN Mei CHE Guixiang ZHOU Jie JIANG Jiequn QIN Zhongyi LIU Xiaoling
    2024, 40(12):227-237. DOI: 10.13982/j.mfst.1673-9078.2024.12.1496
    [Abstract](3) [HTML](0) [PDF 3.72 M](3)
    Abstract:
    To optimize the process of softening crayfish (Procambarus clarkii) shells, the effects of malic, acetic, and citric acids crayfish shell softening were evaluated based on indicators such as the decalcification rate, shell hardness, and sensory score of softened crayfish tails. Multiple regression models were established using the Box-Behnken response surface methodology, with the acid concentration, soaking time, and solvent-to-solid ratio as independent variables and decalcification rate and hardness of crayfish shells as dependent variables to optimize the process of using acid to soften crayfish shells. The results showed that organic acids reduced the hardness of crayfish shells and that the dried products of softened crayfish were accepted by consumers when the decalcification rate of crayfish shells reached at least 48.00%. Among the three organic acids, citric acid was the most effective softener of crayfish shells. When the mass concentration of citric acid, soaking time, and solvent-to-solid ratio were 70 g/L, 63.84 min, and 2.01:1 (V/m), respectively, the decalcification rate and hardness of softened crayfish shells were 56.21% and 14.36 (a reduction of 48.99%), respectively. After acid treatment, the hardness of crayfish shells decreased significantly, and the palatability of crayfish was increased, increasing the appeal of the crayfish products to consumer. The findings provide a theoretical basis and technical support for producing ready-to-eat shell-on crayfish products.
    26  Effect of Carrageenan and Glutamine Transaminase on the Quality Characteristics of Smoked Beef Sausage with Pleurotus eryngii
    HOU Jianbiao ZHU Chaozhi BAI Xueyuan LI Xuan GUO Qi ZHAO Gaiming HAO Jiangang
    2024, 40(12):238-251. DOI: 10.13982/j.mfst.1673-9078.2024.12.1280
    [Abstract](3) [HTML](0) [PDF 8.09 M](2)
    Abstract:
    In order to study the optimal process and formula of smoked Pleurotus eryngii beef sausage, this study took the smoked Pleurotus eryngii beef sausage as the research object, the effects of the addition amounts of Pleurotus eryngii, TG enzyme and carrageenan as well as the mixing time on the sensory evaluation score, texture, yield, moisture content and water-holding capacity were studied, and the optimal formula of smoked Pleurotus eryngii beef sausage was obtained through optimization and analysis by the response surface test. The results of single factor tests showed that all the factors had significant (P<0.05) effects on the sensory score and yield of beef smoked and cooked sausage. The sensory quality (63.46), yield (87.06%) and water-holding capacity (85.55%) of the sausage were the best when the addition amount of TG enzyme was 0.6% (P<0.05). When the addition amount of carrageenan was 0.3%, the sensory score of smoked Pleurotus eryngii beef sausage was the highest (64.84), and the sausage had good texture characteristics and the yield was 107.95%. The response surface test showed that when the addition amounts of Pleurotus eryngii, TG enzyme and carrageenan were 10.00%, 0.65% and 0.30%, respectively, the sensory score of beef smoked and boiled sausage was the best (75.56), and a high yield (110.60%) was obtained, indicating its suitability for industrial production. Taken together, TG enzyme and carrageenan can improve the sensory quality and yield of smoked Pleurotus eryngii beef sausage. The optimal formula of smoked Pleurotus eryngii beef sausage was: addition amount of Pleurotus eryngii, 10.00%; addition amount of TG enzyme, 0.65%; addition amount of carrageenan, 0.30%. This research provides a theoretical basis for the study of smoked and cooked beef sausage.
    27  Comparisons of the Development of Tomato Seed Oil Specialty Shredded Pepper Products and Their Antioxidant Properties
    LAI Huining LI Ling HUANG Siying WEI Xinyi OU Yongxin HE Sijia ZHAO Zhengang
    2024, 40(12):252-259. DOI: 10.13982/j.mfst.1673-9078.2024.12.1276
    [Abstract](3) [HTML](0) [PDF 3.19 M](3)
    Abstract:
    In this study, tomato seed oil and different varieties of dried pepper (Erjingtiao pepper, Qinjiao pepper, Xinjiang roujiao pepper and Xinjiang line pepper) were used to combine with specialty shredded pepper products to investigate their antioxidant capacity and sensory quality. By measuring the physicochemical indexes of tomato seed oil, it was found that its acid value was 1.40 mg (KOH)/g, and the content of unsaturated fatty acids was as high as 78.81%. The DPPH method, ORAC method and PSC method were used to evaluate the antioxidant activity of pepper microemulsion and the changes in the antioxidant capacity of pepper before and after pepper frying. The results showed that the half-inhibitory concentrations (IC50 values) towards DPPH free radical for the microemulsions of Erjingtiao pepper, Qinjiao pepper, Xinjiang roujiao pepper and Xinjiang line pepper were lower than that of tomato seed oil. The ORAC value of Xinjiang roujiao pepper microemulsion was 0.82 μmol TE/g, which was higher than that of tomato seed oil. The highest scavenging ability for peroxyl free radical was with the microemulsion of Erjintiao pepper, with its PSC value being increased by 50.39%. The antioxidant capacity of the pepper was generally reduced after frying, especially the Xinjiang roujiao pepper and Qinjiao pepper. Sensory evaluation showed that Qinjiao pepper products were the most popular. This study provides theoretical support for the development of distinct tomato seed oil shredded pepper products and highlights the importance of selecting appropriate raw pepper materials.
    28  Structural Properties and Antioxidant Capacity of Different Composite Modified Amino Starches
    AN Fengkun HE Linlin WEI Qianyu FU Zhen
    2024, 40(12):260-270. DOI: 10.13982/j.mfst.1673-9078.2024.12.1247
    [Abstract](3) [HTML](0) [PDF 4.65 M](2)
    Abstract:
    In this study, corn starch was used as the raw material, ethylenediamine and o-phenylenediamine were used as the amination reagents, four amino starches were synthesized by two modification methods, crosslinking-etherificationamination, and crosslinking-oxidation-amination, and their structural and functional properties were compared. It was found that the amino starch prepared through introducing o-phenylenediamine via crosslinking-oxidation-amination had the highest nitrogen content (5.97%) and amino content (3.07%), smooth particle/granular appearance, relatively high crystallinity (5.43%) and solubility (11.31%), relatively high starch chain decomposition temperature (220 ℃) and short-range ordering (0.63), and the highest DPPH• and ABTS+• scavenging efficiencies within 1 h (27.90% and 58.12%, respectively). The amino starch introduced with ethylenediamine via crosslinking-oxidation-amination had the highest crystallinity (6.02%) and solubility (91.45%), but the lowest starch chain decomposition temperature (170 ℃) and short-range order (0.46). In addition, blocklike structure was generated in this amino starch. The granular structure of the amino starch synthesized by the cross-linking-etherification-amination method was seriously damaged, with its crystalline structure disappearing completely, and solubility and antioxidant capacity being poor. In summary, the amino starch with o-phenylenediamine introduced via cross-linkingoxidation-amination have excellent structural and functional properties, and high potential for drug loading and heavy metal adsorption. The above results provide a certain reference for the application development of amino starches.
    29  Quality and Functional Properties of Flour Products Fortified with Bamboo Shoot Powder
    ZHANG Zhenwei JIANG Jing XIE Yue ZENG Li JIA Lirong
    2024, 40(12):271-280. DOI: 10.13982/j.mfst.1673-9078.2024.12.1531
    [Abstract](3) [HTML](0) [PDF 2.52 M](2)
    Abstract:
    This study investigated the effects of bamboo shoot powder on the quality and functional properties of flour products including steamed buns, noodles, and biscuits. The products were prepared from wheat flour fortified with varying mass fractions of bamboo shoot powder (0%, 1%, 2%, 3%, 4%, 5%). Steamed buns were used to clarify the effects of the mass fraction of bamboo shoot powder on the whiteness, specific volume, texture, and sensory properties, while noodles were evaluated to determine the effects of the mass fraction of bamboo shoot powder on the texture, stretching, cooking, and sensory properties of noodles. Biscuits were used to determine the effects of the mass fraction of bamboo shoot powder on the color, specific volume, texture, and sensory properties of biscuits. The results showed that the comprehensive quality of steamed buns, noodles, and biscuits was the highest when the mass fraction of bamboo shoot powder was 2%. For steamed buns, biscuits, and noodles made from wheat flour fortified with 2% bamboo shoot powder, the cholesterol adsorption capacity reached 41.55, 31.01, and 33.22 mg/g, respectively; the NO2- removal capacity reached 37.93%, 41.33%, and 25.95%, respectively; and the cation exchange capacity reached 128.00, 133.33, and 122.67 mmol/g, respectively, showing significant improvements compared with the corresponding control groups. Furthermore, functional properties, such as the water-holding capacity, oil-holding capacity, and sodium cholate adsorption capacity, also improved compared to the corresponding control groups. In summary. fortification with an appropriate mass fraction of bamboo shoot powder can significantly improve the quality and functional properties of flour products, potentially aiding the development and utilization of bamboo shoot powder and nutritional enhancement of flour products.
    30  Comparison on the Quality of Frozen Pork Meatball Products Thawed by Different Methods
    ZHU Wenzheng HUAI Xue ZHAO Zhao ZHANG Xinyu XU Yan ZHOU Xiaoyan
    2024, 40(12):281-288. DOI: 10.13982/j.mfst.1673-9078.2024.12.1342
    [Abstract](2) [HTML](0) [PDF 2.18 M](2)
    Abstract:
    In this study, the influences of five different thawing methods, cold thawing at 4 ℃, ultrasound-assisted thawing, running water thawing, air-oscillation thawing and microwave thawing, on the edible quality of frozen pork meatball products were compared. The water activity, texture characteristics and color of the thawed pork meatballs were analyzed. The research results showed that the increase in b* value of the pork meatball in the ultrasound-assisted- thawing group was the least, followed by the microwave thawing group (increases were 0.29 and 0.4, respectively). The changes in the hardness of pork meatballs subjected to different thawing methods differed highly significantly (P<0.01), with the hardness of the pork meatball subjected to running water thawing and microwave thawing being lower (5.65 N and 5.75 N, respectively). There was also a highly significant (P<0.01) difference in the change of chewiness, with microwave thawing causing the least chewiness, and the result of the chewiness similar to that of hardness. Meanwhile, there was a highly significant (P<0.01) difference in the water activity of the pork meatballs subjected to different thawing processes, and ultrasound-assisted thawing and microwave thawing made the water binding in the pork meatball more tightly after thawing, which was conducive to the assurance of product quality. Therefore, ultrasound-assisted thawing and microwave thawing are more conducive to reducing the loss of mouthfeel after pork meatball thawing. Considering the actual thawing conditions of industrial production and households, ultrasonic ultrasound-assisted thawing or running water thawing can be considered for industrial production. Microwave thawing is the best method for thawing pork meatball at home, which can easily maintain the original mouthfeel and flavour of pork meatball. This study is expected to provide theoretical support for the industrial production of pork meatball.
    31  Optimization and Analysis of Polyphenol Extraction from Apple Peels using Ultrasound Assisted Natural Deep Eutectic Solvent
    TIAN Yongtao CHEN Jun XIN Yuyang LIU Tianyue YAO Yamei ZHOU Mengmeng ZHANG Jiawei WANG Wenshu
    2024, 40(12):289-300. DOI: 10.13982/j.mfst.1673-9078.2024.12.1372
    [Abstract](4) [HTML](0) [PDF 4.69 M](2)
    Abstract:
    The extraction process of polyphenols from apple peels by ultrasound-assisted natural deep eutectic solvent (NADES) was established, and the mechanism of NADES was analyzed. After evaluating the polyphenol content of apple peel and antioxidant activity extracted with six NADES and 80% methanol, choline chloride-ethylene glycol (ChCl-EG, 1:4) was identified as the optimal extraction solvent. The process was optimized using single factor and orthogonal experiments, resulting in a solid-liquid ratio of 1:50, a water content of 30%, an extraction duration of 50 min, and an extraction temperature of 60 °C. The extract exhibited significantly higher levels of total phenolic content (TPC), DPPH, ABTS+ free radical scavenging activity, and the ferric ion reducing antioxidant power at 33.16 ± 0.43 mg GAE/g DW and 52.60, 38.30, and 30.76 mg TE/g, respectively, compared to the 80% methanol extracts. These values experienced significant increases of 160.69%, 122.41%, 138.04%, and 102.37%, respectively, following the optimization process. These results indicate that ChCl-EG can extract polyphenols efficiently from apple peels. A total of 17 phenolic compounds were identified from ChCl-EG and methanol extracts using UPLC-MS/MS, with the primary components being consistent. NMR hydrogen spectrum revealed the formation of hydrogen bonds between ChCl and EG molecules. FT-IR analysis indicated that the hydrogen bonding interactions between ChCl-EG and extract molecules was stronger compared to that of the methanol extracts. The analysis of the conductor-like screening model for real solvents (COSMO-RS) revealed that ChCl-EG exhibited superior binding properties with apple polyphenols, with the solubility and interaction energy being 1.39 and 1.12 times those of methanol, respectively. This elucidates the efficient mechanism of ChCl-EG at the molecular level. The results of this study provide a scientific basis for the green extraction technology of polyphenols from apple peel and the efficient mechanism of NADES.
    32  Stability of Red Pigment in Bougainvillea Petals: Impact of pH, Temperature, and Chemical Agents
    LI Li LUO Lixiu QIU Ran WANG Xiangyu SHAO Shujuan WU Jianhang ZONG Xuyan
    2024, 40(12):301-308. DOI: 10.13982/j.mfst.1673-9078.2024.12.1448
    [Abstract](4) [HTML](0) [PDF 3.34 M](3)
    Abstract:
    The stability of the red pigment in Bougainvillea petals was examined during key processes such as extraction, preservation, and application, focusing on factors including heating, pH adjustment, and water removal for enzyme and microbial inactivation. The stability of the pigment was vulnerable to these conditions throughout the process. Using a spectrophotometric method, it was determined that the pigment exhibits maximum stability at a pH of 7, a temperature of 60 ℃, and when stored in the dark. The stability of the pigment was more significantly affected by the oxidant hydrogen peroxide than by the reducing agent sodium sulfite. The addition of stabilizers, such as sodium benzoate, sodium bicarbonate, citric acid, vitamin C, and potassium sorbate at a concentration of 0.4 mol/L, helped mitigate the impact of the oxidant on pigment stability. Metal ions such as lead(II), aluminum(III), and sodium(I) did not significantly affect the stability of the pigment. However, silver(I) and bismuth(II) increased the absorbance of the pigment, producing a rosy red color. Copper(II) caused the pigment to turn orange-red, and without stabilizers, iron(III) caused the pigment to turn blood-red. Avoiding contact with these metal ions is recommended during experiments. These findings provide valuable insights for the practical application of Bougainvillea red pigment.
    33  Ultrasound-assisted Alkaline Extraction of Black Soldier Fly (Hermetia illucens) Larval Protein and Analysis of Its Nutritional Value
    XU Jiahao XIAO Shan HU Wenfeng
    2024, 40(12):309-318. DOI: 10.13982/j.mfst.1673-9078.2024.12.1375
    [Abstract](3) [HTML](0) [PDF 6.84 M](2)
    Abstract:
    As a sustainable protein source, insect protein can be widely used in food, nutraceuticals, and feed to replace traditional animal protein, ensure environmental protection, and alleviate the food crisis. In this study, the ultrasound assisted alkaline method was optimized for the extraction of protein from black soldier fly larvae and the nutritional value of the extracted protein was analyzed. Based on the results of single-factor experiments, the protein extraction process was optimized using a Box-Behnken response surface test designed with four factors and three levels. The optimal extraction conditions, which were determined in combination with the actual production, were as follows: ultrasonic power of 342 W, time of 60 min, NaOH concentration of 24.5 g/L, a liquid-solid ratio of 56 mL/g, and a total volume of 75 mL. The theoretical maximum extraction rate was 82.61%. The validation value of the three parallel experiments was 80.37%. The isoelectric point of the larval protein from black soldier fly was 4.0 and the crude protein content was 80.75%. The content of various essential amino acids in black soldier fly larvae protein were higher than those of the amino acid scoring model for adults proposed by the Food and Agriculture Organization of the United Nations in 2013. In conclusion, an ultrasound-assisted alkaline extraction method for isolating black soldier fly larvae protein was optimized in this study. The prepared protein samples met the amino acid requirements of adults. Thus, black soldier fly and other insects may have application value in the food industry.
    34  Differences in Aroma Characteristics of Four Types of Xinjiang Naan Baked in Different Types of Ovens Based on HS-SPME-GC-MS Analysis
    WANG Lufei AN Bifang BAI Yujia FENG Zuoshan
    2024, 40(12):319-330. DOI: 10.13982/j.mfst.1673-9078.2024.12.1327
    [Abstract](3) [HTML](0) [PDF 2.96 M](2)
    Abstract:
    To investigate the differences between key aroma characteristics of Xinjiang naan baked in tunnel and traditional tandoor ovens, four types of Xinjiang naan, which is a staple food in Xinjiang (sesame naan, onion naan, white naan, and wowo naan) were subjected to aroma detection and analysis using headspace solid-phase microextraction-gas chromatography-mass spectrometry. A total of 50 aroma components were detected in all naan samples, while eight key differential aroma components were identified through relative odor activity value and orthogonal partial least squares-discriminant analyses. The key differential aroma components of sesame naan were benzaldehyde, phenylacetaldehyde, and 2,5-dimethylpyrazine, those of onion naan were dipropyl disulfide and furan, those of white naan were hexanal, nonanal, 2,5-dimethylpyrazine, and 2,6-dimethylpyrazine, and those of wowo naan were benzaldehyde and phenylacetaldehyde. The relative contents of the key differential aroma components of the naan baked in tandoor ovens (ZMN-01, YCN-01, BN-01, and WWN-01) were higher than those of the naan baked in tunnel ovens (ZMN, YCN, BN, and WWN). Specifically, ZMN had a stronger odor of bitter almond, caramel, and herbs than that of ZMN-01; YCN had a stronger onion odor than that of the YCN-01; BN had a stronger nutty roasted odor than that of BN-01; WWN had a stronger odor of bitter almond and caramel than that of WWN-01. This study provides a theoretical reference for the flavor unification of Xinjiang naan baked in different types of ovens.
    35  Low-Temperature Continuous Phase Transition Extraction of Artemisia argyi Levi. et Vant. Oil and Its Composition Analysis
    LIU Siwan GAO Xiangyang
    2024, 40(12):331-338. DOI: 10.13982/j.mfst.1673-9078.2024.12.0995
    [Abstract](3) [HTML](0) [PDF 3.85 M](2)
    Abstract:
    A method was developed for extracting volatile oil from Artemisia argyi using low-temperature continuous phase transition extraction. Based on single-factor experiments, optimal extraction conditions were determined using response surface methodology to achieve the maximum yield as follows: extraction time of 73 min, extraction pressure of 0.45 MPa, and extraction temperature of 50 ℃. Under these conditions, the oil yield reached 4.65%, 3.98% and 3.87% higher than that observed using traditional supercritical CO2 extraction and steam distillation, respectively. The oil extracted using the proposed method was compared with that from the two traditional extraction methods using gas chromatography-mass spectrometry and infrared spectroscopy to detect major chemical composition and quality differences. The results revealed significant differences in the chemical compositions of A. argyi volatile oils obtained using different extraction methods. Thirty-two compounds were identified in the product of low-temperature continuous phase transition extraction, and the oil was mainly composed of volatile terpenoids and long-chain esters. Long-chain esters and alkanes were the major constituents of the oil obtained using supercritical CO2 extraction. Finally, the major components in the oil extracted via steam distillation were volatile terpenoids with low boiling points. All three oil products contained camphor, caryophyllene, palmitic acid, phytol, and linolenic acid. Sensory evaluation results revealed that the oil extracted via continuous phase transition had a high yield, good quality, strong fragrance, and high contents of active compounds, making it suitable for large-scale industrial production.
    36  Comparison of Flavor Differences in Ginseng Tea Prepared with Different Brewing Methods Using Electronic Tongue and Non-targeted Metabolomics
    WEI Fei LUO Shu SONG Yi LI Fang YANG Yuan LUO Xia
    2024, 40(12):339-350. DOI: 10.13982/j.mfst.1673-9078.2024.12.1547
    [Abstract](3) [HTML](0) [PDF 6.79 M](2)
    Abstract:
    Sensory evaluation, electronic tongue, and non-targeted metabolomics techniques were used to investigate the effects of different brewing methods (boiling, simmering, and cold brewing) on the flavor and composition of ginseng tea, a traditional Chinese medicine compound tea used as a food and medicine. The results of the sensory evaluation showed that the scores of ginseng tea brewed using different methods were similar; thus, further differentiation was performed using electronic sensory techniques. The response values of the seven taste sensors of the electronic tongue varied, with significant differences in sour taste, indicating that the flavor of ginseng tea prepared using different brewing modes varied widely and had distinct gustatory characteristics. More than 9 000 metabolites were detected using liquid chromatography-mass spectrometry in the two modes, of which 58 metabolites with significant differences were shared among the three groups in the positive ion mode, and 42 metabolites with significant differences were shared among the three groups in the negative ion mode. The classification statistics of the metabolites showed that lipids and lipid-like molecules accounted for the highest proportion in the positive ion mode (32.6%), followed by heterocycles (15.5%), whereas phenylpropanoids and polyketides accounted for the highest proportion in the negative ion mode (23.8%), followed by lipids and lipid-like molecules (16.7%). Twelve metabolic pathways were obtained in the two modes. Differences were observed in both electronic tongue and liquid chromatography-mass spectrometry identification results of ginseng tea under different brewing modes. Comprehensive analysis indicated that temperature is an important factor leading to differences in the acidic taste of the electronic tongue and expression of organic acid compounds. This study serves as a valuable resource for identifying the flavor characteristics of ginseng tea and for the further development and utilization of medicinal herbs such as Ganoderma lucidum.
    37  Mineral Element Contents in Different Organs of Cattle from Different Regions Evaluated using ICP-MS
    TAN Yufan ZHUANG Peihan CHENG Wenyi ZHAO Wenwei WANG Mei YIN Zhina
    2024, 40(12):351-360. DOI: 10.13982/j.mfst.1673-9078.2024.12.0646
    [Abstract](3) [HTML](0) [PDF 13.59 M](2)
    Abstract:
    The effects of different environmental factors on enrichment of mineral elements in different organs of cattle were analyzed, and the nutritional value of different types of beef were evaluated. Using inductively coupled plasma mass spectrometry, ten mineral elements were analyzed in different organ samples of three species of cattle, namely yaks in Nagqu and Lhasa as well as yellow cattle in Guangzhou. The enrichment levels of trace elements in cattle were comprehensively evaluated by comparing the elemental contents in beef from Nagqu, Lhasa, and Guangzhou, each representing a unique geographical environment. Samples from Naqu, Lhasa, and Guangzhou showed significant differences. The highest calcium content was found in the tripe (455.33~700.25 mg/kg). The highest potassium content was detected in the muscle, ranging from 4 000.46 to 3 650.86 mg/kg. The potassium content of yak meat from the Nagqu region was significantly higher than that of the yak meat from Lhasa and Guangzhou. The sodium content in the muscle was relatively low, ranging from 580 to 698 mg/kg. The sodium content in the muscle of cattle in the Guangzhou area was significantly higher than that in yak meat from the Tibetan area. The magnesium contents in the muscle, heart, and liver were significantly higher than those in the kidney, tripe, and lung. Beef from the Naqu area showed the highest iron content (127.51 mg/kg). The maximum zinc content in the muscle was 170.56~185.56 mg/kg. The highest copper content was detected in the liver, ranging from 11.65 to 16.31 mg/kg. Overall, the mineral elements in different organs of cattle in the Naqu area were higher than those in cattle from the Lhasa and Guangzhou areas. Some major and trace mineral elements widely vary in beef from different origins, which can be used as an index for fingerprint and traceability analysis of meat.
    38  Rapid Determination of Bellkute Residues in Foods of Plant Origin by Ultra-Performance Liquid Chromatography-tandem Mass Spectrometry
    MIAO Lu HUANG Yunlin XIONG Biyu TUO Xiongxin HUANG Hai CHEN Xi
    2024, 40(12):361-368. DOI: 10.13982/j.mfst.1673-9078.2024.12.1527
    [Abstract](7) [HTML](0) [PDF 3.51 M](2)
    Abstract:
    A rapid analysis method for the determination of Bellkute residues in foods of plant origin by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established. The unique enhanced resolution scan (ER) mode of QTRAP was used to solve the difficulty of finding the parent (precursor) ions for multi-charged compounds. The effects of pretreatment, consumable selection, and matrix effects on the recovery rate were investigated, and 20% (V/V) methanol aqueous solution [containing 1% (V/V) formic acid] was selected for extraction. Guanidine hydrochloride (0.5 g) was added and polypropylene was used as the experimental material, to reduce the adsorption of the target by plant components and experimental consumables. The Kinetex F5 column was used for separation, and the quantitative analysis was performed in the positive ion and multiple reaction monitoring mode. The results showed that Bellkute had a good linear relationship in the range of 0.1~100.0 μg/L, and the correlation coefficient r was not lower than 0.998. Using nine plantderived foods such as citrus and asparagus and three spiking levels, the average recovery rate of Bellkute was determined to range from 75.2% to 103.5%, with the relative standard deviations (RSD, n=6) being 2.1%~14.3%, and the limit of detection (LOD) and limit of quantification (LOQ) being 0.6 μg/kg and 2.0 μg/kg, respectively. This method can be used for rapid extraction, and qualitative and quantitative detection of Bellkute residues in foods of plant origin, with advantages of simple operation, high sensitivity, and low cost, thereby being suitable for daily screening and confirmation of Bellkute residues in large quantities of foods of plant origin.
    39  Occurrence and Risk Assessment of Polyhalogenated Carbazoles in Seafood from the Coastal Areas of Zhejiang Province
    WU Yu JIANG Lingbo GAO Zhuoyao NI Lubo DAI Yifei LIU Ting LU Lingzi ZHAO Qiaoling
    2024, 40(12):369-376. DOI: 10.13982/j.mfst.1673-9078.2024.12.1486
    [Abstract](3) [HTML](0) [PDF 3.58 M](1)
    Abstract:
    In this study, gas chromatography-triple quadrupole tandem mass spectrometry was used to study the distribution characteristics of polyhalogenated carbazoles (PHCZs) in seafood. A total of 389 samples belonging to 24 species collected from the coastal areas of Zhejiang Province were investigated to examine their PHCZs concentration, o and a preliminary dietary exposure risk assessment was carried out in combination with the consumption of aquatic products by the residents in Zhejiang Province c. The results showed that the detection rates of PHCZs in all the seafood samples were 100%, with the content ranging from 0.43 to 18.44 μg/kg (Wet Weight; ww). The contents of PHCZs varied among the different kinds of sea food products, that is, the mean value of Σ9PHCZs in fish samples, shellfish samples, and molluscs samples were 5.58 μg/kg ww, 3.35 μg/kg ww, and 4.56 μg/kg ww, respectively. In addition, 3,6-bromocarbazole was the monomer with the highest detection rate and concentration in seafood. The risk assessment revealed that the toxic equivalent was lower than the maximum limit set by the European Food Safety Commission, and the dietary exposure values were lower than the acceptable monthly intake set by the Food Additives Commission. Taken together, the toxicity of PHCZs in seafood from the coastal areas of Zhejiang province is relatively low, the risk of dietary exposure is small, and the leve; of PHCZs is generally at a safe level.
    40  Peptide Composition in Skipjack Tuna Identified Using LC-MS/MS Peptidomics
    GUAN Jingjing FENG Yongqiang YI Jiawen LUO Donghui ZHOU Chunxia CHEN Jin JIANG Guili
    2024, 40(12):377-385. DOI: 10.13982/j.mfst.1673-9078.2024.12.0956
    [Abstract](3) [HTML](0) [PDF 8.57 M](2)
    Abstract:
    The polypeptide compositions of three enzymatic hydrolysis products of skipjack tuna were studied, and the peptide segment length was identified using liquid chromatograph-tandem mass spectrometry peptidomics. The results revealed 2 395 peptide segments identified from three components, among which 266 and 1 342 peptides were identified from peptides consisting of 3~7 and 8~25 amino acids, respectively. Most identified peptides contained 8~18 amino acids. The ion scores of peptides with 3~7 amino acids were -0.207~207, whereas the most scores were in the range of 20.7~124. The ion scores of peptides with 8~25 amino acids ranged from 29 to 517, with the majority between 29 and 224. The correlation between each component was greater than 0.8, indicating a strong correlation. Glutamate acid showed the highest polypeptide content in the enzymatic hydrolysate of skipjack tuna. Myosin heavy chain contributed the largest number of peptide segments. Myosin heavy chain and α-actin exhibited a relatively high protein abundance. Flavor prediction was performed for some peptide segments with high ion scores. LVEEELD was the most frequently detected umami amino acid fragment, with a frequency of 1.428 6. This fragment contained multiple active segments, such as D, E, EE, EEE, EL, EEL, and VE, and had high taste richness. Some bitter amino acids were constituents of umami peptides, whereas sour and sweet amino acids also contributed to the umami taste. Finally, adding the enzymatic hydrolysate of skipjack tuna to other seasoning products influenced the flavor. These results provide a theoretical basis for the development of seasonings related to skipjack tuna.
    41  Rapid Determination of Main Physicochemical Indexes of Cantonese Soy Sauce in the Fermentation Process Based on Near-infrared Spectroscopy
    HUANG Huadan DENG Jianhao CAO Yong LIU Zhan FU Jiangyan KAN Qixin WEN Linfeng XU Ting HU Yuanshu HE Liping
    2024, 40(12):386-401. DOI: 10.13982/j.mfst.1673-9078.2024.12.0716
    [Abstract](3) [HTML](0) [PDF 6.13 M](2)
    Abstract:
    The production of soy sauce is crucial in the food industry. This study aimed to achieve the rapid detection of eight physicochemical indexes of Cantonese soy sauce, namely the content of amino acid nitrogen, total nitrogen, soluble salt-free solids, total acid, ammonium salt, total sugar, reducing sugar, and salt. To achieve this goal, first, quantitative prediction models were established for these physicochemical indexes using near-infrared spectroscopy (NIRS). Specifically, the NIRS spectra of Cantonese soy sauce were processed with five spectral preprocessing methods and two feature band screening methods, and the processed spectra were used to construct quantitative prediction models through partial leastsquares regression (PLSR) and support vector regression (SVR). Next, the performances of the models were compared to screen the optimal quantitative prediction models. The results showed that compared with the PLSR-based quantitative prediction models, the SVR-based quantitative prediction models had higher coefficients of determination (R2) and lower root-mean-square errors, indicating that the SVR-based quantitative prediction models were superior after abnormal sample removal, preprocessing, and feature band screening. The R2 values of the training and test sets for each physicochemical index were between 0.991 1~0.962 1 and 0.977 9~0.857 9, respectively. Furthermore, the optimal quantitative prediction models screened for each physicochemical index was externally validated. The absolute error between the predicted and chemical values of each index of the Cantonese soy sauce was ≤ 1.31, and the t-test results showed that there was no significant difference between the two groups of data, indicating that the quantitative prediction models can quickly and accurately detect each physicochemical index of the Cantonese soy sauce. The proposed models can quickly and nondestructively determine eight important physicochemical indexes of Cantonese soy sauce in the fermentation process, laying a foundation for quality control in the actual industrial production process of soy sauce.
    42  Research Progress on the Occurrence and Intervention Effect of Intestinal Microflora and Its Metabolites in Obesity
    ZOU Lirui GUO Fangrui XU Qiurong WEN Lixin WANG Ji
    2024, 40(12):402-416. DOI: 10.13982/j.mfst.1673-9078.2024.12.0973
    [Abstract](3) [HTML](0) [PDF 2.49 M](5)
    Abstract:
    Obesity is a major global health problem determined by genetics and environmental factors. More and more evidence shows that gut microbiota is related to the occurrence and development of obesity. Gut microbiota is involved in the metabolism of various nutrients such as carbohydrates and lipids in the body, and gut microbiota disorder leads to insulin resistance, adipose tissue accumulation and metabolic abnormalities, causing the occurrence of metabolic diseases, such as high-abundance beneficial bacteria in the human intestinal tract (e.g. Bifidobacterium and Lactobacillus), can prevent the occurrence of obesity by strengthening the intestinal barrier, improving insulin sensitivity and ameliorating metabolic disorders. Many of these effects are mediated by metabolites produced by the intestinal flora, such as short-chain fatty acids and secondary bile acids. On the other hand, species such as Escherichia coli and Staphylococcus can promote the occurrence and development of obesity through harmful metabolites like lipopolysaccharides, peptidoglycans and trimethylamines. In this paper, the correlation between intestinal microbiota and its metabolites, and obesity is reviewed, including Lactobacillus, Akkermansia muciniphila, Bifidobacterium and Enterobacter clocae, as well as the main metabolites of intestinal microbiota. The relationship between intestinal microbiota/ metabolites and obesity is analyzed from the aspects such as microbiota structure, enzymes, cytokines, and signal transduction. This review aims to provide a comprehensive understanding of the relationship between gut microbiota/its metabolites and obesity, and to provide a scientific reference for future clinical intervention and prevention of obesity.
    43  Research Progress on the Application of Non-thermal Physical Sterilization Technology in Cold Chain Food
    ZHAO Songsong WANG Hongyu WU Zijian LIU Jianyu FANG Cheng DAI Quanyu
    2024, 40(12):417-425. DOI: 10.13982/j.mfst.1673-9078.2024.12.1421
    [Abstract](3) [HTML](0) [PDF 2.48 M](2)
    Abstract:
    The low temperature environment of cold chain food effectively inhibits the proliferation of pathogenic microorganisms, but its inhibition and inactivation effects on special pathogenic bacteria such as psychrophilic bacteria are limited, and there are potential food safety hazards. With the rapid development of non-thermal food processing technology in the food industry, research on non-thermal physical sterilization technologies in the field of cold chain food has currently become a hotspot. Non-thermal physical sterilization methods include irradiation, ultraviolet rays, ozone, highvoltage electric field, pulsed magnetic field, and low-temperature plasma treatment. Based on the characteristics of efficient sterilization and no thermal effect during processing, non-thermal physical sterilization technologies can effectively resolve the challenges related to low-temperature bacterial sterilization and quality loss reduction for the cold chain foods such as fruits and vegetables, fresh meat and dairy products. In this paper, a focus is placed on the research progress of the application of non-thermal physical sterilization technologies in cold chain food from the perspectives of the mechanism of action, sanitizing effect on bacteria and microorganisms, and impact on quality. Furthermore, the synergistic application effects and development trends of various non-thermal physical sterilization technologies are summarized herein to provide a reference for the research, development and application of efficient and safe sterilization technologies for cold chain food in the future.
    44  Research Progress on the Physicochemical Properties, Detection Techniques, and Gastrointestinal Function of A1 and A2 β-Casein in Dairy Products
    SUN Yilin YANG Xinyan MAN Chaoxin ZHANG Yu JIANG Yujun
    2024, 40(12):426-434. DOI: 10.13982/j.mfst.1673-9078.2024.12.1350
    [Abstract](3) [HTML](0) [PDF 2.38 M](3)
    Abstract:
    Milk is rich in protein, with approximately 80% of it being casein, which is present in liquid milk in the form of casein micelles. Based on the differences in the amino acids at corresponding positions in the β-casein molecular structure, β-casein has a variety of subtype structures, with A1 and A2 β-casein being the predominant forms. In recent years, there has been a growing interest in the influence of β-casein genetic variants on the physicochemical properties and functional health benefits of dairy products. Single nucleotide gene mutation affects the isoelectric point and properties of β-casein, thereby influencing the structure and physicochemical properties of various dairy products during processing. β-casein is the main source of bioactive peptides, generating numerous amino acids and active peptides through the process of hydrolysis. Variations in the structure of β-casein affect the pattern of digestion enzymatic cleavage, leading to the generation of different types and amounts of peptides through hydrolysis. A1 β-casein, when subjected to enzymatic hydrolysis, results in the formation β-casomorphin-7, which is more likely to adversely affect gastrointestinal digestion and absorption compared to A2 β-casein. This paper provides a comprehensive review of the effects of micellar structure and stability, gel coagulability, and interface properties of A1/A2 β-casein on the processing of dairy products. The effects of A1 and A2 β-casein on the gastrointestinal tract after digestion have been described. Furthermore, a comprehensive analysis was conducted to systematically assess the opportunities and challenges still existing in this field, aiming at providing theoretical guidance for future development of the dairy products market.
    45  Research Progress of Roses and Predictive Analysis of Their Quality Markers
    XI Jiayu LIU Wenjing SHAO Shijun LIU Hong FENG Jinliang YANG Fude
    2024, 40(12):435-442. DOI: 10.13982/j.mfst.1673-9078.2024.12.1512
    [Abstract](3) [HTML](0) [PDF 1.98 M](2)
    Abstract:
    Rose, a traditional qi-regulating Chinese medicine, has the effects of moving qi, alleviating depression, harmonizing blood and relieving pain. As a class of food and medicine homologous Chinese medicines, roses have a history of cultivation and planting in China for more than 2 000 years and are widely distributed. Roses contain a variety of chemical components, mainly including volatile oils, polyphenols (e.g. flavonoids), polysaccharides, proteins and amino acids, etc. Modern pharmacological research showed that roses exhibit strong physiological activities in antioxidant, anti-bacterial, hypoglycemic, hypolipidemic, anticancer, antitumor, cardiovascular disease prevention and antidepressant aspects. In this paper, the resource distribution, chemical composition and pharmacological effects of roses are reviewed. The potential quality markers of roses are predicted from the aspects of plant genealogy, specificity of chemical composition and its predictability, traditional medicinal efficacy and medicinal properties, and the correlation between chemical composition and pharmacological effects. The results indicate that geraniol, citronellol, phenyl ethanol, trans-2-heptanol, 2-methylpentanoic acid, 1,1-diethoxyethane, 2,6,10,15-tetramethyldecapeptadecane, apigenin, kaempferol, and quercetin can be used as potential quality markers for roses, in order to provide a reference for the quality control of roses.
    46  Matcha: History, Health Benefits and Potential Risks
    LI Wenxuan Fang Qiting Shi Xiujiao Hu Meijuan Ye Jianhui
    2024, 40(12):443-455. DOI: 10.13982/j.mfst.1673-9078.2024.12.1414
    [Abstract](3) [HTML](0) [PDF 2.72 M](2)
    Abstract:
    Matcha is a powder made from tea leaves and has a unique green tea flavor and color. Matcha has been widely used in beverage and food production for its health benefits and food processing properties. This study reviews the development history, physicochemical properties, and potential health benefits of matcha, including anti-inflammatory effects, promotion of cognitive improvement, and alleviation of metabolic syndrome symptoms. The potential plasticizing risks of matcha upon full utilization of tea leaves were discussed relative to each extraction method and detection technique. This discussion suggests that a stable and reliable method for analyzing phthalates in tea products must be developed to assess the associated risks and effectively control plasticizers in matcha.

    Editor in chief:李琳

    Inauguration:现代食品科技

    International standard number:ISSN 1673-9078

    Unified domestic issue:CN 44-1620/TS

    Domestic postal code:46-349

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