Volume 40,Issue 10,2024 Table of Contents

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  • 1  Advances in Evaluation of Rice Starch Digestibility and the Intrinsic and Extrinsic Factors Affecting the Same
    LI Yingying LI Xia YANG Jie
    2024, 40(10):1-13. DOI: 10.13982/j.mfst.1673-9078.2024.10.1205
    [Abstract](10) [HTML](13) [PDF 2.69 M](10)
    Abstract:
    The glycemic index (GI) is an effective indicator of the postprandial glycemic response, and is of great importance for guiding the dietary choices of individuals with obesity or diabetes. Rice is the staple food of more than half of the world's population. Consumption of a low GI rice diet to achieve a low and stable postprandial glucose response is a cost-effective means of controlling the blood glucose levels. In vitro digestion simulates the physiological conditions present during digestion. Compared to in vivo digestion experiments, in vitro digestion allows us to study the GI characteristics of food in a rapid, inexpensive, and reproducible manner. This paper summarizes the research progress on the concept of rice GI, the methods used for measuring rice GI, the digestion models used for evaluating the digestibility of rice starch, and the interactions between rice starch granules and non-starch components (intrinsic characteristics) and processing factors (external factors). It is necessary to further study the genetics of resistant starch, establish standardized cooking processes, conditions, and heat-moisture treatment processes, and strategies for delaying or inhibiting starch retrogradation to enable the acquisition of rice with low digestibility. As the current in vitro digestion model can only estimate the rice GI, future research should be targeted at continuously improving this model to enable it to closely simulate in vivo digestion.
    2  Processing Technology, Physicochemical Properties, and Flavor of Low-eGI Reconstituted Rice
    LYU Qingyun CHEN Kuan JIANG Lei ZHANG Yunchen LIU Liangzhong ZHU Zhe GAO Hongbo ZHAO Pengjie
    2024, 40(10):14-25. DOI: 10.13982/j.mfst.1673-9078.2024.10.0162
    [Abstract](5) [HTML](9) [PDF 6.39 M](12)
    Abstract:
    The processing technology, physicochemical properties, and flavor of low-estimated glycemic index (eGI) reconstituted rice were investigated. Using a twin-screw extruder, a low-GI reconstituted rice suitable for consumption by diabetic patients was prepared from regular white rice flour as the main ingredient and white kidney beans, chickpeas, and mulberry leaf extracts as excipients. The optimal extrusion process parameters were determined to be a screw speed of 110 r/min, temperature of 116 ℃ , water addition of 23%, and feed rate 18 kg/h using one-way tests and response surface methodology for optimization. Using these parameters, the overall rating was 85.2 and the eGI of the rice was 53.81. The gelatinization, textural, cooking, and flavor properties of the low-eGI reconstituted rice were subsequently compared with those of regular white rice, The results revealed that the hardness, absorptive capacity, and solubility of the low-eGI reconstituted rice were all significantly higher than those of the white rice. Furthermore, scanning electron microscopy observations revealed that compared with the regular white rice, the low-eGI reconstituted rice was characterized by a rougher surface and a looser more porous structure. In terms of flavor, the low-eGI reconstituted rice was established to have larger amounts of specific flavor compounds that imparted a unique fragrance and reduced odor. Our findings accordingly provide fundamental information to enhance the manufacturing and quality of low-eGI reconstituted rice.
    3  Comparative Analysis of Fine Structures for Phytoglycogen and Fungal Glycogen
    LI Enpeng LI Tong DING Zhen LI Songnan
    2024, 40(10):26-31. DOI: 10.13982/j.mfst.1673-9078.2024.10.1280
    [Abstract](4) [HTML](11) [PDF 2.51 M](9)
    Abstract:
    Phytoglycogen and fungal glycogen exist in mutant plants and fungi, respectively, and they are closely related to physiological processes, such as growth and development, metabolic pathways, and the stress response. A comparative analysis was conducted on the fine structures of three types of phytoglycogen and four types of fungal glycogen using size-exclusion chromatography, fluorescence-assisted carbohydrate electrophoresis, and transmission electron microscopy. The results showed that phytoglycogen exhibited a unimodal (10~20 nm) full molecular distribution, while fungal glycogen mainly presented a bimodal (10~20 nm and>40 nm) full molecular distribution. Fungal glycogen had a higher average chain length (8.7~10.4 DP) than phytoglycogen (4.4~6.9 DP), Both phytoglycogen and fungal glycogen exhibited a cauliflower-like morphology. Phytoglycogen was mainly composed of β particles (10~20 nm), whereas fungal glycogen contained both α particles (50~180 nm) and β particles. These results showed that fungi not only have large glycogen molecules that act as storage materials, but also small glycogen molecules that can quickly release energy; however, plant mutants only have small, unstable phytoglycogen molecules. The findings of this study will help our understanding of the differences in biological functions and evolutionary relationships between phytoglycogen and fungal glycogen.
    4  Raw Material Formulation Optimization of Low GI Composite Extruded Rice and Verification of its Hypoglycemic Function
    WANG Xing JIANG Lei CHEN Kuan ZHANG Yuncheng LIU Liangzhong ZHU Zhe LYU Qingyun CHANG Xianhui DING Wenping
    2024, 40(10):32-39. DOI: 10.13982/j.mfst.1673-9078.2024.10.1133
    [Abstract](6) [HTML](14) [PDF 1003.08 K](8)
    Abstract:
    Here, low glycemic index (GI) composite extruded rice was prepared using twin-screw extrusion technology, with rice flour as the main ingredient and white kidney beans, chickpeas, mulberry leaf extract, and soy protein isolate (SPI) as auxiliary ingredients; further, the hypoglycemic function of this rice was verified in mice. Optimization of the raw material formulation of the low GI composite extruded rice using the response surface method revealed the following addition percentages to be the best: white kidney beans, 39.3%; chickpeas, 30.4%; mulberry leaf extract, 4.6%; SPI, 1.0%. Among these, white kidney beans and chickpeas significantly affected the GI of the raw materials of the composite extruded rice. The inhibition rates of the low GI composite extruded rice in the context of α-amylase and α-glucosidase activity were 33.4% and 53.2%, respectively. Experiments in diabetic mice showed that continuous consumption of the low-GI composite extruded rice could effectively reduce fasting blood glucose levels, maintain blood glucose balance, and significantly improve the glucose tolerance in a mouse model of streptozotocin-induced diabetes. This study provides ideas for research on extruded rice with high nutritional value and good quality so as to meet the low GI staple food needs of individuals with diabetes.
    5  Preparation and Characterization of Extruded Rice with a Low Glycemic Index
    LIU Yubin MA Qihang YAN Jin LUO Zhen ZHANG Yuan WANG Jinmei
    2024, 40(10):40-49. DOI: 10.13982/j.mfst.1673-9078.2024.10.0680
    [Abstract](3) [HTML](12) [PDF 5.22 M](11)
    Abstract:
    Rice is the primary staple food for people in China. However, people with obesity and the diabetes are discouraged from consuming rice due to its high glycemic index (GI). A low-GI extruded rice was prepared using the extrusion technique. The rice had good appearance, quality, and taste. Extrusion technique was employed to ensure that the extruded rice closely resembles natural rice. Rice flour was used as the main material, and a mixture of mulberry leaf extract and resistant starch was added for their bioactive function of GI reduction. Monoglycerides, oat bran, and soybean polysaccharides were added to modify the properties of the extruded rice. The glucose release rate during in vitro digestion significantly decreased (from 0.060 to 0.025 min-1) when the mixture of mulberry leaf extract and resistant starch was added, and the estimated GIs for the finished extruded rice samples were ≤ 46.93. Moreover, their structure was compact and tightly packed and their morphology was intact. The addition of monoglycerides and oat bran enhanced the hardness of the extruded rice and reduced its adherence, whereas the addition of soy polysaccharides improved its appearance and made the extruded rice softer and more glutinous. The total sensory evaluation score for the extruded rice prepared with soybean polysaccharides, mulberry leaf extract, and resistant starch as the functional components was the highest, and its sensory characteristics were closest to that of natural rice amongst the modified, low-GI versions. This suggests that this preparation method is superior and more suitable for low-GI extruded rice.
    6  Changes in Structural, Physicochemical, and Digestive Properties of Chinese Wild Rice Starch before and after Heat-moisture Treatment
    ZHANG Haifeng CHEN Yansong XU Ruoyuan YUAN Meng YIN Li LI Chunmei
    2024, 40(10):50-59. DOI: 10.13982/j.mfst.1673-9078.2024.10.1461
    [Abstract](27) [HTML](15) [PDF 3.28 M](22)
    Abstract:
    Here, the effects of heat-moisture treatment (HMT) on the structural, physicochemical, and digestive properties of Chinese wild rice starches with different moisture contents (HMT-15, HMT-20, HMT-25, HMT-30) were investigated. Results showed that under conditions of HMT, the morphology of starch granules in wild rice was damaged to varying degrees, the crystalline structure of starch was altered, and short-range ordering was reduced; these phenomena became more pronounced as the moisture content of the system increased. The particle size of starch increased from 0.84 μm to 5.14 μm, and the relative crystallinity of starch decreased from 26.29% to 20.65%. The solubility of starch derived from Chinese wild rice increased from 19.25% to 24.01%, and the swelling degree decreased from 15.13 g/g to 12.11 g/g. The freeze-thaw stability of this starch tended to first increase and then decrease. The syneresis rate of HMT-20 (35.12%) was the highest, while that of HMT-30 (13.12%) was the lowest (significantly lower than even that of natural wild rice starch (19.66%)). Analysis of the gelatinization properties showed that HMT increased the gelatinization temperature of wild rice starch and reduced the viscosity and retrogradation of the wild rice paste system. In vitro digestion showed that in Chinese wild rice starch, the content of rapidly digestible starch (RDS) decreased significantly, while that of resistant starch (RS) increased significantly with increasing moisture content in the system; HMT-30 had the lowest RDS content (20.49%) and the highest RS content (28.66%). Chinese wild rice starch exposed to HMT under conditions of high moisture exhibited robust stability in both hot and cold conditions, and good aging resistance and digestive resistance. The results of this study would provide new ideas for the further processing and promotion of Chinese wild rice starch.
    7  In Vitro Digestive Characterization of Formulated Foods with Different Raw Material Compositions and Determination of Their eGI Values
    WANG Huaibin LIU Jia WANG Meng AN Haifeng CHEN Jing LI Xiang YU Wenwen
    2024, 40(10):60-67. DOI: 10.13982/j.mfst.1673-9078.2024.10.1284
    [Abstract](25) [HTML](16) [PDF 1.32 M](19)
    Abstract:
    in recent years, carbohydrate foods with a low glycemic index (GI) have attracted attentions from consumers due to their health benefits, especially their ability to maintain stable postprandial blood glucose levels. With the increasing demand for specialized medical-purpose formula foods (referred to as formula foods) among individuals with specific health conditions, developing in vitro methodologies/models to accurately estimate the GI values, as an alternative to labor intensive in vivo blood test, has become of essentially important in the research and promotion of low-GI foods. Therefore, this study has systematically investigated the in vitro digestion characteristics of 10 formula foods with distinct ingredient compositions, obtaining various kinetics digestion parameters. These kinetics parameters were further analyzed in relation to human blood glucose test data, so that mathematical equations/models could be established to enable rapid prediction of their GI values. Results showed that the in vitro digestion characteristics varied significantly across different sample compositions. Among all digestion parameters, AUC90 (the area under the reducing sugar release curve within 0~90 min) demonstrated the greatest potential for estimating the eGI values of all samples. The predictive equation for GI values is given by eGI=0.020 6×AUC90-20.618 0 (R²=0.908 1). Moreover, it was also noted that the impacts of carbohydrate composition on eGI values differs depending on the ingredient type. Both solid corn syrup and tapioca dextrins significantly increased the concentration of reducing sugars, with comparable contributions to postprandial blood glucose elevation. In contrast, xylitol had a potentially smaller impact on blood glucose than maltitol. Additionally, the moderate inclusion of fructose did not show a significant effect on eGI values.
    8  Relationship between Multiscale Structure and in Vitro Digestive Properties of Modified Lotus Seed Starch
    XIE Yaman ZHANG Nan PAN Juan LI Yukun LIU Chuang FU Shuyong YANG Wenan GAO Xiangyang
    2024, 40(10):68-78. DOI: 10.13982/j.mfst.1673-9078.2024.10.1271
    [Abstract](20) [HTML](7) [PDF 5.39 M](26)
    Abstract:
    To investigate the mechanism of the impact of the structure of lotus seed resistant starch on digestibility, lotus seed starch was modified using different physical treatments (autoclaving, ultra-high pressure, microwave) combined with pullulanase. The results showed that the particle size of the modified lotus seed starch increased, the surface of the particles was rough and formed irregular lumps, the “Maltese cross” formation disappeared, the relative crystallinity (from 31.02% to 18.19%) and short-range ordering (1.56 to 0.97) significantly decreased (P<0.05), and the crystalline type changed from C-type to B-type. In vitro digestion experiments confirmed that starch treated with ultra-high pressure combined with enzymatic modification contained the highest content of high-resistant starch (56.50%). The digestion properties of lotus seed resistant starch were significantly correlated with the branched amylose chain length distribution and the short-range ordering structure and crystalline structure. The increase in short-range ordering, relative crystallinity, and B1 chain and B2 chain content of the starch molecules was conducive to the enhancement of the resistance of lotus seed resistant starch to enzymatic hydrolysis, which may be related to the regulation of digestive properties. The relationship between the multiscale structure of modified lotus seed starch and its digestive properties, were analyzed herein to provide a reference for the preparation of lotus seed resistant starch with strong anti-digestive ability using novel food processing technology, and to guide the production of low-GI lotus seed starch food with hypoglycemic function.
    9  Near-infrared Model for Quantifying Rehmannia glutinosa Polysaccharide Content and its Anti-inflammatory Effect on Kidney Injury in Obese Mice
    LI Dongyang HUANG Fengyu MENG Xinyu LIU Qingpu LEI Jingwei XIE Caixia
    2024, 40(10):79-88. DOI: 10.13982/j.mfst.1673-9078.2024.10.1160
    [Abstract](25) [HTML](15) [PDF 1.58 M](26)
    Abstract:
    A method for the rapid determination of the Rehmannia glutinosa polysaccharide (RGP) content by near infrared spectroscopy (NIRS) was established. The effects of different RGP concentrations on inflammation in obese mice were investigated. The NIR diffuse reflectance spectra of Rehmanniae Radix Praeparata were collected and analyzed using the partial least squares (PLS) method. The spectra were processed using the multivariate scatter correction (MSC), direct difference, and first order derivative methods within a wavelength range of 4 040.12~11 221.66 cm-1. The quantitative analysis model for evaluating the RGP content was established by taking into consideration 19 principal components; the predicted contents were 34.63%~45.28%, the measured contents were 34.49%~45.09%, the absolute deviations were -0.41%~0.26%, and the relative deviations were 0.121%~0.995%. The obese mice model was generated using a high-fat diet. Renal function index (RFI), renal histopathology, and fasting blood glucose (FPG), total cholesterol (TC), triglyceride (TG), and creatinine (Cr) levels were evaluated after 14 weeks of RGP administration at various doses. The body weight, TC, TG, Cr, FPG, and RFI in the high-RGP content group decreased by 11.29%, 31.59%, 47.63%, 34.63%, 28.57%, and 19.00%, respectively (compared with the model group). RGP could significantly attenuate structural damage and inhibit inflammatory cell infiltration in kidney tissues, and these effects were positively correlated with the RGP content. These results showed that the NIRS model can quickly and accurately determine the RGP content, and that RGP can ameliorate dyslipidemia, inhibit the kidney inflammatory response, and protect the renal structure in obese mice. The findings of this study can help sunstantiate the development and utilization of RGP.
    10  Modulatory effect of Glycerol Monostearate Concentration on the Structure and Delivery Properties of a Bigel System Embedded with Probiotics
    ZHANG Tiantian LIU Guoqin
    2024, 40(10):89-96. DOI: 10.13982/j.mfst.1673-9078.2024.10.1172
    [Abstract](16) [HTML](11) [PDF 2.78 M](26)
    Abstract:
    A water-in-oil bigel structure was constructed with a glycerol monostearate (GMS)-based oleogel and a xanthan-gum-based hydrogel to encapsulate Lactobacillus plantarum in the internal aqueous phase. The structure of the bigel system was modulated by changing the concentration of GMS. The microstructure, mechanical properties, and rheological properties of the system were characterized. The protective effect of the system on the viability of probiotics was explored through simulated in vitro digestion experiments. The results showed that the homogeneity, stress resistance, crystallinity, and system stability of the bigel samples improved with increasing GMS concentration; however, the system stability decreased when the GMS concentration exceeded 10%. After gastrointestinal digestion in the simulated in vitro gastrointestinal digestion experiments, no viable probiotics were detected in the free probiotics samples, while the counts of viable probiotics were higher than 7.5 log CFU/g in all samples with probiotics embedded in the aqueous phase of the bigel system. The count of viable probiotics after gastrointestinal digestion and their release in the small intestine varied with the GMS concentration, with higher probiotic viability and release in samples with higher GMS concentrations. These results suggest that regulating the GMS concentration in the bigel system can modulate the viability of the embedded probiotics and their release in the intestine. This study provides a theoretical basis for the development of probiotic delivery systems and functional foods.
    11  Ameliorating Effects of Free Phenolic Extract of Inonotus hispidus on Hyperuricemia
    LIN Junhui ZHAO Kefang LIAO Sentai PANG Daorui ZOU Yuxiao
    2024, 40(10):97-104. DOI: 10.13982/j.mfst.1673-9078.2024.10.1143
    [Abstract](18) [HTML](10) [PDF 1.53 M](20)
    Abstract:
    In recent years, there has been an increasing incidence of hyperuricemia, which affects seriously human health. The results of previous studies have shown that the free phenol extract of Inonotus hispidus has a strong in vitro uric acid-lowering activity, but there is still a lack of in vivo experimental verification. Accordingly, a hyperuricemia mouse model was established in this study by feeding the mice a high uric acid mouse feed combined with the gavage of oteracil potassium for 4 weeks, in order to determine the in vivo uric acid-lowering effect of the free phenolic extract of Inonotus hispidus. The results showed that after 4 weeks of intervention with the extract, the levels of serum uric acid, serum urea nitrogen and serum adenosine deaminase, and the activity of hepatic xanthine oxidoreductase of the mice in the high-dose group of Inonotus hispidus free phenolic extract [200 mg/(kg•bw)] were significantly reduced (by 65.99%, 43.68%, 67.31%, and 89.73% respectively, P<0.05) compared to the model group, with its regulatory effectbeing comparable to that of allopurinol (positive drug control). In addition, the free phenol extract also demonstrated a certain restorative effect on the cell damage of the kidney tissue caused by hyperuricemia. Therefore, the Inonotus hispidus free phenolic extract may have a good uric acidlowering effect. The results of this study can provide a theoretical reference for the development of novel uric acid-lowering functional food resources using Sanghuangporus spp.
    12  Supercritical CO2 Extract of Pine Pollen Inhibits Benign Prostatic Hyperplasia in Mice
    SONG Shihao YIN Liduan LYU Suyuan WANG Tong WU Qiu ZHAO Fa LIANG Xiuqing GENG Yue
    2024, 40(10):105-112. DOI: 10.13982/j.mfst.1673-9078.2024.10.1011
    [Abstract](25) [HTML](10) [PDF 2.38 M](17)
    Abstract:
    In this study, supercritical CO2 fluid technology was used for the extraction of Yunnan pine pollen, and the effect of the obtained extract on the benign prostatic hyperplasia (BPH) in mice was investigated. It was found that the effects of extraction time, temperature, flow rate, and pressure on the yield (corresponding effects ranked from large to small), with the optimal yield being 9.9%. Gas chromatography-mass spectrometry (GC-MS) analysis of the extract indicated that organic acids accounted for 50.63% of the total compounds, followed by alkanes, alcohols, and others. Testosterone propionate was injected intraperitoneally to establish a mouse BPH model. Pine pollen extract (0.8 mg/kg•d) daily was daily gavaged for 28 days. The results showed that compared to the control group (CON), the prostate weight and prostate index of the BPH group increased significantly, the epithelial cells in the prostate tissue thickened and protruded inwardly, the contents of interleukin-6 (IL-6), tumor necrosis factor-α(TNF-α), dihydrotestosterone (DHT), prostate acid phosphatase (PAP) and superoxide dismutase (SOD) increased, and the contents of interleukin-10 (IL-10) and caspase-3 (CASP-3) decreased. Based on metabolomics analysis, the metabolic pathways such as amino acid-tRNA biosynthesis, L-phenylalanine and tyrosine biosynthesis, and tryptophan biosynthesis underwent differential changes. Following intervention with the pine pollen extract, the aforementioned conditions were reversed to varying degrees. These indicated that pine pollen extract can alleviate testosterone propionate-induced benign prostatic hyperplasia by regulating inflammatory factors, BPH pathways, proliferation and apoptosis, oxidative stress, and metabolic pathways. It provides a theoretical support for the development and utilization of pine pollen resources.
    13  Analysis and Evaluation of Nutritional and Chemical Components of Tylopilus microsporus
    XIE Sha ZHAO Qiuyu YANG Fazhong YANG Xiaoqin LI Yunxian
    2024, 40(10):113-124. DOI: 10.13982/j.mfst.1673-9078.2024.10.1229
    [Abstract](23) [HTML](9) [PDF 1.41 M](19)
    Abstract:
    Tylopilus microsporus belongs to the Boletaceae family, and is sourced from Luliang County, Qujing City, Yunnan Province. The analysis results of the nutritional composition of Tylopilus microsporus shows that Tylopilus microsporus is rich in nutrients, and the main nutritional components (g/100 g) were: crude protein 29.9, crude polysaccharide 3.68, essential amino acid 11.18, and total amino acids 23.80. The essential amino acids to non-essential amino acids (E/N) index was 0.89, and the essential amino acids to total amino acids (E/E+N) was 46.97%. The total essential amino acids meet the FAO/WHO standard mode, with balanced nutritional composition. The EAAI value (essential amino acid index) was 1.05. Tylopilus microspores was rich in mineral nutrients, mainly including Ca, K, Mg, P, Fe, Cu, Mu and Se, in particular, the K content was high. A total of 949 metabolites were identified from Tylopilus microsporus, in which 798 metabolites, including 10 superclasses, matched to the human metabolic database. There were multiple active metabolites with relatively high contents. Tylopilus microsporus is a wild bolete with high protein content and low sugar content. Its amino acid content was high and had a relatively complete range of species. Meanwhile, the content of bitter amino acids and potassium content provide an important basis for bitter research and development utilization of new boletes.
    14  Improvement Effect of a Composite Peptide on Wound Healing in a Mouse Full-thickness Skin Defect Model Based on Metabolomics Analysis
    YU Jingting CHENG Xiaoying ZHAN Yang WAN Jianhua LI Yingmeng LIU Wenjun
    2024, 40(10):125-135. DOI: 10.13982/j.mfst.1673-9078.2024.10.1142
    [Abstract](24) [HTML](15) [PDF 7.71 M](25)
    Abstract:
    A mouse full-thickness skin defect model was established to analyze the healing effect of a composite peptide comprising wheat oligopeptide and marine fish skin collagen oligopeptide. On the 4th and 14th days after the intervention, pathological changes in the wound were assessed by subjecting the skin taken from the back of mice to H&E staining; the extent of angiogenesis was determined based on CD34 expression (estimated by immunofluorescence). Serum metabolomic profiles were obtained using ultra-high-performance liquid chromatography and mass spectrometry to screen for differential metabolites whose expression was significantly altered. Metabolic profile analysis, OPLS-DA analysis, and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed. On the 4th, 6th, and 8th days after the injury, the wound healing rates in the composite-peptide group reached 59%, 74.1%, and 80.9% respectively, which were significantly higher than those observed in the model group. Furthermore, the granulation tissue continued to mature, and higher counts of CD34-positive cells were observed in the composite-peptide group. Metabolomic analysis showed that among differentially expressed metabolites in the composite-peptide group, 14 were upregulated and 20 were downregulated. KEGG pathway analysis showed that the composite peptides were significantly enriched for six pathways, i.e., taurine and hypotaurine metabolism, arginine and proline metabolism, and primary bile acid biosynthesis (compared to the model group). Thus, composite peptides can reduce the repair time of full-thickness skin defects in mice, possibly by promoting capillary formation and protein biosynthesis to accelerate wound healing.
    15  Analysis of Differences in Bacterial Community Structure and Physicochemical Indices between Yellow and Black High-temperature Daqu Produced in Handan
    WANG Yurong HOU Qiangchuan TIAN Longxin ZHANG Zhendong HUANG Like KONG Jinzhao HAO Guangfei GUO Zhuang
    2024, 40(10):136-145. DOI: 10.13982/j.mfst.1673-9078.2024.10.1024
    [Abstract](17) [HTML](8) [PDF 6.82 M](19)
    Abstract:
    Illumina MiSeq high-throughput sequencing was used to analyze the differences in bacterial community structure between yellow and black high-temperature Daqu. Subsequently, the physicochemical indices of Daqu samples were measured, and the correlations between bacterial community structure and physicochemical indices were analyzed. Finally, the pure culture technique was used to explore the bacterial populations present in the Daqu samples. The sequencing results showed that the average relative abundances of Thermoactinomyces (21.04%), Limosilactobacillus (14.30%), and Weissella (3.15%) in the yellow high-temperature Daqu were significantly higher (P<0.05) than those (1.93%, 0.036%, and 0.027%, respectively) in the black high-temperature Daqu, whereas the average relative abundances of Lentibacillus (3.30%) and Kroppenstedtia (2.81%) in the yellow high-temperature Daqu were significantly lower (P<0.05) than those (34.45% and 20.75%, respectively) in the black high-temperature Daqu. In addition, LEfSe analysis showed that Thermoactinomyces and Lentibacillus could be used as biomarkers for yellow and black high-temperature Daqu, respectively. The measurement results of physicochemical indices showed that compared to the black high-temperature Daqu, the yellow high-temperature Daqu exhibited significantly higher water content, liquefaction power, saccharification power, esterification power, and alcoholic power (P<0.05), and significantly lower acidity (P<0.05). Correlation analysis revealed significant correlations between the dominant bacterial genera and physicochemical indices in the two types of high-temperature Daqu (P<0.05). A total of 33 bacterial strains were isolated from high-temperature Daqu samples using the pure culture technique. The strains comprised ten species under five genera, with Bacillus being the dominant genus and B. licheniformis being the dominant isolate (accounting for 30.30% of the total number of isolates). Hence, the two types of high-temperature Daqu exhibited significant differences in bacterial community structure and physicochemical indices, and their bacterial community structures were correlated with their physicochemical indices.
    16  Isolation and Identification of Molds on the Surface of River Snail Rice Noodles
    Liu Qun Xiong Xiaodi Liu Xing Lin Hua Li Li Zhang Lixiang Chen Naizhong Liu Tao
    2024, 40(10):146-153. DOI: 10.13982/j.mfst.1673-9078.2024.10.1169
    [Abstract](18) [HTML](13) [PDF 1.37 M](18)
    Abstract:
    Mold contamination can adversely affect the quality of river snail rice noodles. To clarify the mold biodiversity, molds on the surface of river snail rice noodles were isolated and identified, and their growth characteristics (colony morphology, spore germination, and mycelial growth) were analyzed. Comparison of ITS-rDNA sequences and NCBI sequences revealed the isolation of 32 mold species under 11 mold genera, with five dominant mold species identified, namely Penicillium sp. (6.3%), Cladosporium sp. (40.6%), Alternaria sp. (9.4%), Aspergillus sp. (9.4%), and Talaromyces sp. (9.4%). The phylogenetic tree showed that these isolates exhibited high similarity (>99%) with the known mold species. Penicillium sp. exhibited the highest spore germination and mycelial growth rates, whereas Aspergillus sp. and Cladosporium sp. exhibited low spore germination rates during the early stage despite their high mycelial growth rates. To the best of our knowledge, this is the first report on the isolation and identification of molds on the surface of river snail rice noodles, revealing a variety of mold species on the surface of rice noodles that indicate potential risks in food quality and safety. The findings of this study can facilitate the development of intervention technology for eliminating mold contamination in river snail rice noodles.
    17  Process Optimization of the High-density Fermentation with Lactiplantibacillus plantarum J26 and its Application to the Fermentation of Soy Milk
    ZHENG Yaping WANG Junhao WANG Chenchen ZHANG Yu JIANG Yujun MAN Chaoxin
    2024, 40(10):154-164. DOI: 10.13982/j.mfst.1673-9078.2024.10.0997
    [Abstract](23) [HTML](15) [PDF 1.59 M](28)
    Abstract:
    Lactiplantibacillus plantarum J26 was used as the research object. The carbon and nitrogen sources were screened using the Biolog GEN III Microstation automated microbial identification system. After optimization, a mixture of 25 g/L maltose and glucose (1:1) was used to replace the carbon source in the MRS medium, and a mixture of 10 g/L beef paste and yeast powder (1:1) was used to replace the nitrogen source in the MRS medium. The optimal fermentation conditions for the strains determined by single factor experiment were as follows: initial pH of the medium, 5.6; fermentation temperature, 33 ℃; inoculation amount, 4%. Under these conditions, a 5-liter fermenter was used for high-density fermentation. Lactiplantibacillus plantarum J26 reached the stable stage after 14 h of fermentation, and the viable cell count could reach 4.35×1010 CFU/mL. Because Lactiplantibacillus plantarum J26 has a high viable cell count and high fermentation activity, thus can be used as a starter for the fermentation of soy milk. Fermented soy milk with a good viable bacterial count (3.56×108 CFU/mL), water-holding capacity (48.12%), and textural characteristics was obtained. By optimizing the highdensity fermentation process, the number of viable bacteria in the fermentation broth can be effectively increased. This provides a theoretical basis for the application of high-density fermentation with Lactiplantibacillus plantarum J26 and the industrialization of fermented soy milk.
    18  Controlled Enzymatic Hydrolysis of Egg Yolk Protein and the Pro-osteoblast Proliferative Activity of the Resulting Hydrolysate
    YAN Jiangang YU Qin LIU Ying DU Yulan XIE Lan CUI Chun MAO Xinliang
    2024, 40(10):165-172. DOI: 10.13982/j.mfst.1673-9078.2024.10.1101
    [Abstract](25) [HTML](12) [PDF 5.80 M](21)
    Abstract:
    Defatted egg yolk powder is as a byproduct of the industrial extraction of egg yolk lecithin. This protein and nutrient-rich product appears to be commercially valuable. To optimize enzymatic hydrolysis, defatted egg yolk powder samples were hydrolyzed using trypsin, with calcium chelating capacity serving as the primary evaluation index. The optimum hydrolysis conditions were identified by coupling unidirectional analysis (involving variables like hydrolysis duration, temperature, pH, and enzyme dosage) to response surface optimization. The pro-proliferative activity of the resulting hydrolysate in the context of MC3T3-E1 osteoblasts was assessed to enable the high-value application of defatted egg yolk powder. The results indicated that under optimum conditions, i.e., hydrolysis duration, 3 h; temperature, 55 ℃; pH, 10.0; enzyme dosage, 1%, the calcium chelating capacity reached 87.12%. The hydrolysate produced under these conditions markedly enhanced the proliferation of MC3T3-E1 osteoblasts. This enhancement was most pronounced at a hydrolysate concentration of 1.00 mg/mL, where the proliferation of the treatment group osteoblasts was more than that of the blank group osteoblasts (by 1.20 times). The findings of this study reveal that the trypsin-facilitated enzymatic hydrolysis of defatted egg yolk powder effectively produces hydrolysates with considerable pro-osteoblast proliferative activity, thereby supporting the high-value application of defatted egg yolk powder.
    19  Extraction of Heat-resistant Lipase from Rice Bran and Preliminary Investigation on the Characteristics of Crude Enzyme
    LIU Chunling PAN Bihui DENG Yuanyuan ZHONG Lihuang ZHANG Yan TANG Xiaojun ZHOU Pengfei ZHANG Mingwei LIU Guang
    2024, 40(10):173-180. DOI: 10.13982/j.mfst.1673-9078.2024.10.1002
    [Abstract](15) [HTML](15) [PDF 1.66 M](18)
    Abstract:
    Rice bran lipase can make rice bran go rancid, and conventional heat treatment methods are difficult to completely inactivate endogenous lipase in rice bran. In order to evaluate the presence of heat-resistant lipase in rice bran, the present study was carried out to investigate the effects of surfactant type and concentration on the lipase activity of rice bran’s crude enzyme solution using the rice bran stabilized by the wet heat treatment as the raw material, and further compare the characteristics of the two lipases, Triton x-100 rice bran lipase (TRBL) and phosphate rice bran lipase (PRBL). The lipase activity of the crude enzyme solution extracted with 10 mmol/L surfactant increased significantly, with the Triton x-100 group exhibiting the best effect. Comparison on the properties of TRBL and PRBL revealed that the optimal reaction temperature and pH of PRBL were 30 ℃ and 7.0, respectively, whilst the optimal reaction temperature of TRBL was over 100 ℃ and it had good heat resistance, with the optimal reaction pH of being 3.0 and 7.0; The two crude liquid lipases had no significant specificity in the hydrolysis of substrates with different carbon chains; Ba2+ and Zn2+ could activate the lipase activity of the crude liquid enzymes, but the activation effect of Ba2+ on the activity of TRBL was much higher than that of the PRBL group. K+ and Fe3+ had the greatest inhibitory effect on the lipase activities of PRBL and TRBL, respectively; TRBL had better tolerance to organic solvents than PRBL, and methanol had an activating effect on TRBL’s lipase activity. The study proved that there were lipases that could tolerate temperature and organic solvents in stabilized rice bran, which provides a theoretical basis for in-depth exploration of novel rice bran lipases.
    20  Dynamic Changes of Microorganisms and Quality in Refrigerated Raw Milk
    QIU Yue CHENG Jingqi HE Mengli GUAN Ning ZHAO Ying YANG Xinyan MAN Chaoxin JIANG Yujun
    2024, 40(10):181-190. DOI: 10.13982/j.mfst.1673-9078.2024.10.1050
    [Abstract](19) [HTML](14) [PDF 3.61 M](17)
    Abstract:
    In this study, the dynamic community structure and abundance of microorganisms in raw milk during low temperature storage (4 ℃, 8 days) were monitored by third-generation 16s rDNA high-throughput sequencing technology, and the metabolic pathways of microbial functional genes in raw milk were analyzed in combination with the database. The results showed that during the low-temperature storage of raw milk, psychrophilic bacteria gradually became dominant bacteria, and their main metabolic pathways, such as carbohydrate metabolism, lipid metabolism and amino acid metabolism, were closely related to the quality changes of raw milk. The increases in the synthesis and metabolism of amino acids and fat transport metabolism eventually led to the deterioration of the flavor, texture and organization of dairy products. During the refrigeration process, the dominant microorganisms dominated by psychrophilic bacteria multiplied in large numbers and produce heat-resistant enzymes, which hydrolyze the protein and fat in the raw milk, causing the deterioration of milk quality.
    21  Comparison of the Physical and Chemical Properties of Collagens Extracted from Tortoise Shell using Three Methods
    HE Xiong YU Zengyan JIANG Shaotong LU Jianfeng LIN Lin
    2024, 40(10):191-199. DOI: 10.13982/j.mfst.1673-9078.2024.10.1066
    [Abstract](16) [HTML](11) [PDF 1.83 M](17)
    Abstract:
    The basic and mineral compositions of the ventral and dorsal shells of tortoise (Chinemys reevesii) were analyzed, and collagens (ASC, PSC, and H-PSG) were extracted from shell samples using acid, enzymatic, and hot-water assisted enzymatic methods. Subsequently, the physical and chemical properties of the extracted collagens were characterized through ultraviolet spectrometry, infrared spectrometry, SDS-PAGE, amino acid analysis, and scanning electron microscopy. The results indicated that tortoise shells had high protein (22.06%~22.50%), calcium (16 004.25~16 592.50 mg/100 g), and phosphorus (6 014.25~6 188.25 mg/100 g) contents. ASC, H-PSG, and PSC yields were 0.45%~0.51%, 4.38%~4.75%, and 1.47%~1.56%, respectively. The maximum absorption peaks of ASC, H-PSG, and PSC extracted from ventral and dorsal shell samples appeared at 220~226 nm. The infrared spectra and SDS-PAGE patterns showed that ASC and PSC were both type Ⅰ collagen with high purity and a relatively intact triple-helix structure, but H-PSG had degenerated into gelatin and had been partially hydrolyzed to collagen peptide. ASC, H-PSG, and PSC extracted from the ventral and dorsal shell samples had essentially the same amino acid composition, with abundant glycine (18.19%~24.10%) and proline (12.52%~15.05%). In terms of microstructure, ASC exhibited a superior three-dimensional network structure compared to H-PSG and PSC. In conclusion, the collagens extracted from the ventral and dorsal shells of tortoise using the same methods showed no significant difference in composition or properties; however, different extraction methods affected the yield, composition, and structure of collagens extracted from tortoise shells.
    22  Structural Characterization and Functional Properties of the Polysaccharides from the Fruits of Nitraria sibirica Pall
    AYITUXUN Abuduwaili SONG Niannian YI Yuxin SAINAWAER Mangsier
    2024, 40(10):200-207. DOI: 10.13982/j.mfst.1673-9078.2024.10.1026
    [Abstract](24) [HTML](9) [PDF 2.70 M](23)
    Abstract:
    The polysaccharides from Nitraria sibirica Pall (NSP-C) fruit was prepared by enzymatic hydrolysis and purified using macroporous adsorption resin. The structure was preliminarily examined by gas-chromatography, liquid chromatography, infrared spectroscopy, scanning electron microscopy-energy dispersive X-ray spectroscopy, X-ray powder diffraction, Congo red staining, thermal analysis, periodate oxidation and the Smith degradation method; The functional properties of NSP-C were evaluated through measuring water- holding capacity, oil-holding capacity, emulsifying ability, emulsion-stabilizing ability, and free radical scavenging ability. NSP-C was composed of rhamnose, arabinose, mannose, glucose and galactose in a molar ratio of 1:1:0.73:2.04:3.75, and had a molecular weight of 5.07 ku. NSP-C had typical polysaccharide infrared absorption peaks, a triple helical structure and an amorphous structure, good thermal stability. NSP-C had good water-/oil-capacity (2.87 g/g and 5.97 g/g, respectively), and showed good emulsifying capacity under alkaline conditions (64.41 m2/g). The half maximal inhibitory concentrations of NSP-C against 2,2-diphenyl-1-picrylhydrazyl and hydroxyl free radicals were 0.209 and 0.149 mg/mL, respectively, indicating strong free radical scavenging ability. In summary, the NSP-C from the fruit of Nitraria sibirica has good functional properties and antioxidant activity, the experimental results can provide a scientific basis for the comprehensive utilization of Nitraria sibirica resources.
    23  Development of A Novel SO2 Sustained-release Pad for Food Preservation and Its Application in Preserving Fresh Noodles
    MIAO Chengdu SONG Yuqing MA Yue LIU Yiwei LIU Hongsheng WU Lei XU Yanyuan WU Junshi LIN Dong
    2024, 40(10):208-218. DOI: 10.13982/j.mfst.1673-9078.2024.10.1112
    [Abstract](14) [HTML](15) [PDF 5.89 M](18)
    Abstract:
    A novel, biodegradable, and cost-effective starch-based foam has been developed for packaging fresh noodle products of high commercial value. The packaging pads were designed to enable the formation of a modulable antimicrobial atmosphere by pre-embedding sodium metabisulfite (at three concentrations: 1.6, 8.0, and 16.0 g/m2) as precursors. An antimicrobial atmosphere showing controlled sulfur dioxide release is created via chemical interactions of the sodium metabisulfite with the environmental moisture. Throughout the storage period, various crucial parameters, including overall appearance, moisture content, color score, total bacterial/mold count, cooking properties, and sulfur dioxide residual content, were continuously monitored. The results indicate that the severity of browning in fresh noodles was reduced when the pad with a sodium metabisulfite concentration of 8.0 g/m2 was used. Additionally, the water loss and absorption rates of the fresh noodles decreased only by 0.9% and 9%, respectively, and the optimum cooking time was shortened only by 70 s. The total bacterial count was maintained at 0.7×105 CFU/g at 48 h, meeting national standards. Thus, the shelf life was extended from 24 h to at least 48 h. Furthermore, an ideal low residual sulfur dioxide concentration (approximately 1.35 mg/kg), which was significantly lower than the national standard safety limit (10 mg/kg), was maintained. The results demonstrate that the preservation pad with a sodium metabisulfite concentration of 8.0 g/ m2 is the most effective in preserving fresh noodles. The present study introduces an innovative solution for preserving fresh noodles and other similar instant foods.
    24  Inhibition of Salmonella typhimurium Growth by ε-Poly-Lysine Hydrochloride and Its Consequent Use in Wet Rice Noodles
    HU Ruiqi ZHENG Jun LIN Yilin YU Yigang
    2024, 40(10):219-226. DOI: 10.13982/j.mfst.1673-9078.2024.10.1316
    [Abstract](27) [HTML](12) [PDF 2.85 M](26)
    Abstract:
    Here, to investigate the inhibitory effect of ε-poly-lysine hydrochloride (ε-PLH) on the growth of Salmonella typhimurium, the minimum inhibitory concentration (MIC) was determined and the growth curve was plotted. The effect of ε-PLH on the cell membrane of S. typhimurium was investigated by evaluating propidium iodide (PI) infiltration and intracellular macromolecule leakage. The effects of ε-PLH on oxidative stress and energy metabolism in S. typhimurium were explored by measuring the intracellular reactive oxygen species (ROS) levels, superoxide dismutase (SOD) and ATPase activities, and ATP content. The results revealed that ε-PLH exhibits robust antibacterial properties in the context of S. typhimurium, with an MIC of 128 μg/mL. Further, ε-PLH was found to effectively inhibit the growth of S. typhimurium. Following treatment with ε-PLH at the MIC, the integrity of the S. typhimurium cell membrane was lost, leading to changes in cell membrane permeability and excessive accumulation of ROS. After 6 h of treatment, the levels of nucleic acid leakage and ROS were 2.45 and 1.99 times higher than those in the control group. Further, compared to the control group, the SOD activity, ATPase activity, and ATP content decreased by 29.60, 10.00, and 67.63%, respectively, in the ε-PLH-treated group. ε-PLH treatment affected the cell membrane of S. typhimurium; this caused oxidative damage and disruption of the intracellular energy balance, ultimately leading to impaired physiological functions and death in S. typhimurium. Furthermore, treatment of wet rice noodles with ε-PLH revealed that ε-PLH still exhibited an antibacterial effect against S. typhimurium and had a good fresh-keeping effect. In conclusion, ε-PLH exhibits promising potential with respect to the control of Salmonella contamination in food.
    25  Effects of Ozone Treatment on Postharvest Storage Quality and Sterilization of Late-maturing Citrus
    LE Liqing XIA Xiaolong LONG Yong CHEN Deyong GAO Li ZENG Xiaodan LI Huajia ZHU Yongqing ZHU Jin HE Hongyu
    2024, 40(10):227-238. DOI: 10.13982/j.mfst.1673-9078.2024.10.1196
    [Abstract](19) [HTML](14) [PDF 2.26 M](21)
    Abstract:
    The late-maturing citrus was used as experimental material to explore the effect of different ozone treatment durations on citrus postharvest storage in an effort to counter the problem of quality decline and fruit decay during the postharvest storage of citrus. The results showed that treatment with fixed ozone concentrations every day (for different durations) for 25 days had a significant impact on the quality of late-maturing citrus. Ozone treatment for 1 min per day could significantly reduce the weight-loss rate of citrus, which was only 59.90% of that of the CK group after storage for 25 days. The contents of total soluble solids (TSS) in citrus were effectively maintained after daily treatment for 10 min; the TSS content was 9.20% at the end of storage. Ozone treatment could delay the decrease in the Vc content of citrus; it significantly inhibited the activities of polyphenol oxidase (PPO), superoxide dismutase (SOD) and catalase (CAT), and reduced the number of microorganisms present on the surface of citrus peel. However, the duration of ozone treatment did not have any significant effect on citrus hardness, titratable acid (TA) content, and solid acid ratio. Treatment for 1, 10, 20, and 40 min daily could inhibit the increase in the malondialdehyde (MDA) content and enhance the activity of peroxidase (POD) in citrus. Treatment for 40 min daily also significantly increased the total polyphenol content (TPC) and total flavonoid content (TFC) of citrus. Principal components analysis showed that the comprehensive quality of citrus treated with ozone for 10 and 40 min daily was better. From the perspective of economy and safety, daily ozone treatment for 10 min could be used as a suitable method for preserving late-maturing citrus fruits during post-harvest storage.
    26  Comparison of the Quality Characteristics of Yam Powder Processed using Different Methods
    GENG Kezan DUAN Yingyu ZHANG Ruifen MA Qin
    2024, 40(10):239-249. DOI: 10.13982/j.mfst.1673-9078.2024.10.1233
    [Abstract](22) [HTML](10) [PDF 2.85 M](21)
    Abstract:
    Here, differences in the blending and digestion characteristics and active ingredient contents of yam powder prepared using four processing methods, i.e., hot air drying, stir-frying with bran, extrusion, and spray drying, were investigated. Results revealed that in terms of blending characteristics, yam powders processed using hot air drying and stir-frying with bran had low caking rates but relatively poor blending and dispersion stability, with the water solubility index (WSI) being 5.33% and 6.67% and thermal stability index (TSI) being 9.67% and 6.09%, respectively. Extrusion and spray drying significantly improved the blending and dispersion stability of yam powder, with WSI of 23.6% and 15.33% and TSI of 2.89% and 3.32%, respectively. In terms of digestive characteristics, the RDS and RS contents of hot air-dried yam powder were 18.16% and 75.49%, respectively, and the in vitro digestibility of yam proteins was 57.99%. Compared with hot air drying, both extrusion and spray drying improved the digestibility of the starches and proteins present in yam powder. The digestion characteristics of the extruded yam powder were the best, with the RDS content, RS content, and in vitro digestibility of yam proteins being 47.37%, 50.35%, and 74.42%, respectively. In terms of the active ingredient content, the polysaccharide content was the highest (4.53%) in extruded yam powder, and the allantoin content was the highest (5.89 mg/g) in spraydried yam powder. These results showed that extruded and spray-dried yam powders had good blending and digesting characteristics, and active ingredients could be well retained. This study can provide substantial technical support for the development of healthy and nutritional foods based on yam.
    27  Optimization of the Preparation of Exocarpium Citri grandis Extract and Its Lipid Level-lowering Effects
    LI Jinkun JIN Gangliang WANG Yanhui XIAN Shaohua ZHAO Junxin YU Junlang LIU Haofan TAN Yaokang WEN Junwei XIONG Ping
    2024, 40(10):250-258. DOI: 10.13982/j.mfst.1673-9078.2024.10.0892
    [Abstract](19) [HTML](19) [PDF 3.02 M](24)
    Abstract:
    The optimal extraction conditions of exocarpium Citri grandis were determined using a single-factor test and an orthogonal test. The effects of water addition, extraction frequency, extraction time, and extraction temperature were investigated; the mass fractions of naringin and dry extract were used as evaluation indexes. Furthermore, a combined hyperlipidemia mouse model was established by feeding mice a high-fat diet to investigate the lipid level-lowering effects of exocarpium Citri grandis extract. The results showed that the optimal conditions for preparing the aqueous extract of exocarpium Citri grandis were as follows: solvent, water; solid-liquid ratio, 1:30; and extraction temperature, 70 ℃; the extraction was performed three times (60 minutes each time). Under these conditions, the naringin content in the aqueous extract of exocarpium Citri grandis was 56.15 mg/g. Traditional processes mainly use ethanol as the solvent; this process can provide a reference for aqueous extraction of Exocarpium Citri Grandis. The high-dose exocarpium Citri grandis group showed 47.11%, 36.84%, and 65.18% lower levels of TC, TG, and LDL-C, respectively, significantly lower liver index and Lee's index values, and 56.68% and 56.63% lower serum ALT and AST levels, respectively, than the model group. The high-dose group also showed milder liver cell swelling and degeneration and reduced lipid droplet deposition and fat vacuole formation. The results indicate that the exocarpium Citri grandis extract exerts lipid level-lowering effects on mice with combined hyperlipidemia.
    28  Effect of Microwave Drying on the Phenolic Content and Color Changes in Different Regions of Persimmon Slices
    HOU Wenyi DUAN Zhenhua YI Yi
    2024, 40(10):259-269. DOI: 10.13982/j.mfst.1673-9078.2024.10.1203
    [Abstract](16) [HTML](10) [PDF 2.60 M](17)
    Abstract:
    Using persimmons as raw materials, the changes in moisture, color, and phenolic composition and content in six regions (A1, A2, B1, B2, C1, C2) of persimmon slices were investigated under fresh and different microwave drying times (11, 13, 15 min), powers (415, 485, 555 W), and loading capacities (2.15, 2.35, 2.55 kg/m2). The results showed that the contents of water, total phenolics, total flavonoids, total flavanols, and persimmon tannins in the A2 region of fresh persimmon slices were the highest at 81.92%, 125.68, 38.58, 12.77, and 111.29 mg/g, respectively. Microwave drying was found to reduce the overall water and phenolic contents in different regions of persimmon slices, and the color changed from yellow to brownish black. During the microwave drying, changes in the B2 region were the most pronounced, with the lowest water content. Browning first occurred in the B2 region and then spread to other regions. Correlation analysis showed that the contents of total phenolics and persimmon tannins were significantly and positively correlated with brightness (L*) (P<0.05) and significantly and negatively correlated with the total chromatic aberration (ΔE) (P<0.05). Microwave drying can cause the color of persimmon slices to deteriorate, and microwave-dried persimmon slices can no longer have the color of fresh persimmon. The color change is related to the self-oxidation of phenolics, and flavonoids and flavanols are not responsible for the color deterioration of persimmon slices. The results of this study can provide a reference for the drying of persimmon. Further studies should be conducted on the regulation of color changes in persimmon from the perspective of phenolics.
    29  Automated Identification of Camellia Shells and Seeds using a Combination of HOG and LBP Features
    DUAN Yufei SUN Jiwei DONG Geng WANG Yanqing
    2024, 40(10):270-275. DOI: 10.13982/j.mfst.1673-9078.2024.10.0864
    [Abstract](20) [HTML](20) [PDF 3.94 M](15)
    Abstract:
    A self-built camellia fruit shell and seed sorting device has achieved rapid sorting of camellia fruit shells and seeds using a HOG-LBP feature fusion method developed for the automated identification of camellia fruit shells and seeds. The device collected shell and seed images, segmented the foreground images of shells and seeds by pre-processing the collected images, and extracted the HOG shape features and LBP texture features of shells and seeds. To improve the detection rate, the dimensionality of HOG features, LBP features, and HOG+LBP features were reduced using principal component analysis (PCA), and the shell and seed classifier was trained using four machine learning algorithms, namely, BP neural network, naive Bayesian model, K-nearest neighbor, and support vector machine. The performance of the trained classifiers were compared with the results from the test set. The experimental results showed that the HOG+LBP features based classifier trained by support vector machine performed best in identification of shells and seeds, with a training accuracy of 100% and an average test accuracy of 96.00%. Moreover, the identification accuracies after feature fusion were all higher than those based on single features, indicating that the proposed method is effective for the automated identification of camellia fruit shells and seeds.
    30  Analysis of Main Quality Components of Guizhou Tencha Collected in Different Picking Periods
    HUANG Nanhuan XU Daihua HAO Jian CHEN Ling GONG Xue LIU Yuan DENG Yanli
    2024, 40(10):276-283. DOI: 10.13982/j.mfst.1673-9078.2024.10.1084
    [Abstract](16) [HTML](11) [PDF 1.78 M](18)
    Abstract:
    In order to investigate the quality difference of tencha collected in different picking seasons under the same shading conditions, the fresh leaves of the same grade Fuding Dabai tea collected in late spring, early summer and midsummer were used as the objects in this study. Relevant biochemical components were detected and analyzed after the teas being processed by the same tencha process. The results showed that with the picking period being moved to a later time, the contents of water extract, tea polyphenols, total chlorophyll, chlorophyll b, TDF, IDF and SDF, the phenol-ammonia ratio and the IDF/SDF ratio increased significantly (P<0.05), and the total free amino acid content, Vc content and the chlorophyll a/chlorophyll b ratio decreased significantly (P<0.05). Principal component analysis showed that the cumulative variance contribution rate of total free amino acids, water extract, SDF, chlorophyll a, soluble sugar and GCG contents reached 95.73%, which could be used as a characteristic component of the quality of tencha. The soluble sugar content (3.00%), total free amino acid content (4.40%), caffeine content (2.57%), Vc content (5.25 mg/g), gallic acid content (1.10%), EGCG content (6.74%), content of total ester-type catechins (9.02%), and content of total catechins (10.40 %) of the tencha N1 harvested at the end of spring were the highest, whilst the tea’s polyphenol content (10.94 %), catechin bitterness and astringency index (16.59), phenol-ammonia ratio (2.49) and IDF/SDF ratio (4.84) were the lowest, thus, the overall quality was the best. Therefore, the quality of tencha processed in late spring was the best, whilst the quality of tencha processed in early summer or mid-summer was relatively poor. This study can provide a scientific theoretical basis for the production and processing technology and quality improvement of Guizhou tencha and matcha.
    31  Quality Evaluation of Different Varieties of Kiwifruit Based on Principal Components Analysis and Cluster Analysis
    LIU Xiaobing
    2024, 40(10):284-292. DOI: 10.13982/j.mfst.1673-9078.2024.10.1242
    [Abstract](18) [HTML](15) [PDF 1.75 M](20)
    Abstract:
    To experimentally analyze the fruit quality of various varieties of kiwifruit, we established a system for evaluating different kiwifruit varieties. Here, 18 varieties of kiwifruit were studied by employing 16 indexes corresponding to appearance and internal nutritional quality. The variability of various nutritional indexes among kiwifruit varieties was analyzed, and the quality of kiwifruit was comprehensively evaluated using principal components analysis (PCA) and cluster analysis. Results showed that there were differences in the quality indexes of 18 kiwifruit varieties, and the coefficients of variation of L*, a*, b*, soluble solid, Vc, dry matter, total flavonoids, total acid, and sugar-acid ratio were all greater than 20%, indicating that there were differences among various varieties of kiwifruit. PCA revealed five principal components for extraction; the cumulative explained variance ratio was 86.851% and the top three varieties were Actinnida deliciosa ‘Bruno’, ‘Cuixiang’, and ‘Nongda Jinmi’. Cluster analysis segregated the 18 kiwifruit varieties into three clusters, with A. deliciosa ‘Bruno’ forming a single cluster. A. deliciosa ‘Bruno’ had the following characteristics: high moisture content, high sweetness, and good taste. The results of this study can provide a reference for the quality evaluation of different kiwifruit varieties and a theoretical basis for the breeding and development of kiwifruit.
    32  Preparation and Pb(Ⅱ) Adsorption Characteristics of the Fibers from Laian Malus asiatica Fruit Pomace
    ZHANG Cuan CHEN Zhihong LIU Yang
    2024, 40(10):293-301. DOI: 10.13982/j.mfst.1673-9078.2024.10.1117
    [Abstract](21) [HTML](13) [PDF 2.46 M](105)
    Abstract:
    In order to explore the physicochemical and functional properties of three kinds of fiber samples, namely WF, AF and AKF, were extracted from Malus asiatica fruit pomace using water, 1.25 g/100 mL hydrochloric acid and 1.25 g/100 mL sodium hydroxide solution. Based on the analysis of the main physicochemical and functional properties of original fruit pomace and derived fiber samples, their Pb(Ⅱ) adsorption properties and mechanism were analyzed and compared. The result shows that the fruit pomace of Malus asiatica contained 27.64 g/100 g crude fiber, and the crude fiber contents in the WF, AF and AKF samples were 48.60, 54.14 and 64.72 g/100 g, respectively. The results of Pb(Ⅱ) adsorption experiments shows that the three fiber samples from fruit pomace had similar adsorption properties. Within the concentration range of 1~ 4 μg/mL, with the increase of the initial Pb(Ⅱ) concentration, both the equilibrium adsorption capacity and adsorption rate increased significantly (P≤0.05). When the initial concentration was 4 μg/mL, the AKF had the highest equilibrium adsorption capacity and adsorption rate (which were 394.86 μg/g and 33.18 μg/min, respectively), followed by AF, with the WF being the lowest. The results of adsorption kinetics and thermodynamics tests showed that the adsorption processes of Pb(II) by the three fibers from the fruit pomacewere more in line with a quasi-second-order kinetic model, with chemical adsorption being the main adsorption mode. The adsorption process was an exothermic reaction, with an appropriate low temperature being conducive to the adsorption. The results showed that fibers from Laian Malus asiatica fruit pomace have good water- and oil-absorption properties, and a strong adsorption ability for Pb(II) in aqueous solutions, which will lead to good application prospects in the fields of functional food additives and water treatment.
    33  Comparison of Nutritional Quality and Antioxidant Activity of Rowan Fruit Wines Subjected to Different Pretreatments
    SUN Dun XUE Guixin
    2024, 40(10):302-310. DOI: 10.13982/j.mfst.1673-9078.2024.10.1190
    [Abstract](25) [HTML](18) [PDF 6.27 M](17)
    Abstract:
    In this research, black fruit glandular-ribbed Rowan fruit (short name: blackfruit) was used as the raw material. Blackfruits were pretreated respectively in five different ways, namely, no pretreatment (CK, as the control), warm water bath treatment (HTWB), apple ripening treatment (AMWA), late harvest treatment (DH), and freezing treatment (LTF), and the fruits were processed into fruit wines, to compare the differences in nutrient content and antioxidant activity of the fruit wines. The results showed that after the comparison of the five fruit wines, the pretreatments decreased significantly the fruit wine’s total acid content and antioxidant activity and caused significant differences in the nutrient contents of the fruit wines. The polyphenol content (8.94 mg/g) and hydroxyl radical scavenging rate (40.9%) of the LTF-treated wine were significantly higher than those of the other fruit wines (P<0.05). The content of total flavonoids (19.23 mg/g), tannin content (0.782%) and superoxide radical scavenging rate (68.08%) of the HTWB-treated wine were significantly higher than those of other fruit wines (P<0.05); the anthocyanin content (0.155 mg/g) and DPPH scavenging rate (97.91%) of the DH-treated wine were significantly higher than those of other fruit wines (P<0.05). The results of principal component analysis showed that among the five pretreatments, the late harvest treatment was the best pretreatment for retaining the nutrients in the fruit wine and its operation was simple and its cost was low, thus, this pretreatment is suitable for wide promotion and application.
    34  Changes in Quality and Flavor Substances of Sichuan White Rex Rabbit Meat during Repeated Freeze-thaw Processes
    PAN Xuerui WANG Ping JIAN Wensu ZHANG Yan CHEN Lianhong
    2024, 40(10):311-326. DOI: 10.13982/j.mfst.1673-9078.2024.10.0956
    [Abstract](22) [HTML](13) [PDF 2.67 M](21)
    Abstract:
    In order to investigate the effects of repeated freezing and thawing on the quality and flavor substances of Szechuan white Rex rabbit meat, the pH, color difference, thiobarbituric acid reactant (TBARS), texture, water-holding capacity (WHC), total sulfhydryl (-SH) content, carbonyl content (CV) and volatile flavor substances of samples undergoing different freezing and thawing cycles were determined. The results showed that with the increase of the number of freezing and thawing cycles, the crude protein and crude fat contents of Sichuan white Rex meat decreased by 6.71% and 2.76%, the L* and b* values increased significantly, the a* value decreased significantly, the pH value decreased from 6.25 to 5.39, the water-holding capacity decreased, and the texture deteriorated. The TBARS value increased from 0.16 mg/kg to 0.65 mg/kg, CV content increased by 64.37%, myofibrillar protein solubility decreased by 35.85%, and -SH content decreased by 60.59%; a total of 149 kinds of volatile flavor substances were detected in the Rex rabbit meat during repeated freezing and thawing processes. and three principal components were obtained by the principal component analysis , with a cumulative contribution rate of 80.37%; The differential volatile discriminant analysis showed that hydrocarbons, aldehydes, alcohols, ketones and acids were the main differential flavor substances, among which the content of hexanal increased in the late stage of the freezing and thawing process, indicating that repeated freezing and thawing promoted the oxidation of proteins and fats, which led to the deterioration of the quality and flavor of Rex rabbit meat. Comprehensive indicators indicated that the number of freezing and thawing cycles of Rex rabbit meat should be controlled within 3 times in actual production. The research results provide a theoretical basis for the storage quality control of Sichuan white Rex meat.
    35  Aging Time-dependent Changes in the Concentrations of Key Components of Liubao Tea Produced Using Different Processes
    MENG Juan YANG Lifang PI Dujuan WANG Fang WENG Jie LIU Xinyue SUN Haibing LIU Hongcun
    2024, 40(10):327-337. DOI: 10.13982/j.mfst.1673-9078.2024.10.1063
    [Abstract](23) [HTML](11) [PDF 2.66 M](21)
    Abstract:
    Aging is an important process that enhances the flavor of Liubao tea by affecting the concentrations of key components, such as water extracts, alkaloids, polyphenols, and polysaccharides. To clarify the effect of aging on the concentrations of these components, samples of Liubao tea prepared using traditional and modern processes and aged for one, three, five, ten, or fifteen years were tested via chemical analysis and high-performance liquid chromatography (HPLC). The results indicated that from one to fifteen years of aging, the tea samples prepared using either of the processes did not show any significant change (P>0.05) with respect to the dry matter content. However, there was a continuous decrease in the flavonoid content (from 16.70 mg/g and 7.93 mg/g in tea samples prepared using the traditional process to 15.67 mg/g and 3.40 mg/g in tea samples prepared using the modern process after one year of aging and fifteen years of aging, respectively). A similar continuous decrease was also observed in the polyphenol content (from 19.93 mg/g and 6.13 mg/g in tea samples prepared using the traditional process to 15.57 mg/g and 4.33 mg/g in tea samples prepared using the modern process after one year of aging and 15 years of aging, respectively). The free amino acid content in the Liubao tea prepared using either of the processes was significantly lower after five years of aging than after one year of aging (P≤0.01) and significantly lower after fifteen years of aging than after ten years of aging (P≤0.01). After five years of aging, the water extract content peaked at 566.37 mg/g and 500.10 mg/g in tea samples prepared using the traditional process and modern process, respectively, which was significantly higher than the corresponding values after fifteen years of aging (P≤0.01). After five years of aging, the polysaccharide content increased to 43.07 mg/g and 34.80 mg/g in tea samples prepared using the traditional process and modern process, respectively, which was significantly higher than the corresponding values (17.73 mg/g and 13.47 mg/g) in tea samples prepared using the traditional process and modern process, respectively, after fifteen years of aging (P≤0.01). The two types of tea samples exhibited similar trends with respect to the concentrations of the key components. However, the transformation of the different components occurred more rapidly in tea samples prepared using the modern process than in tea samples prepared using the traditional process in the early years of aging. After 15 years of aging, the two types of tea samples had essentially the same key compound contents. The metabolism and transformation of key compounds during aging are responsible for the unique qualities of the Liubao tea, i.e., red color, rich fragrance, smooth taste, and mellow flavor. This study provides scientific data for the quality control and evaluation of Liubao tea by analyzing the aging time-dependent changes in the concentrations of the key compounds in Liubao tea.
    36  Comparison of the Flavor Qualities of Chili Oils Prepared from Different Types of Vegetable Oil
    YANG Fang WANG Xuncheng JIA Hongfeng XU Chengjian YUAN Haibin
    2024, 40(10):338-350. DOI: 10.13982/j.mfst.1673-9078.2024.10.0985
    [Abstract](21) [HTML](16) [PDF 11.41 M](23)
    Abstract:
    To investigate the influence of vegetable oil on the physicochemical properties and flavor of chili oil, the capsaicinoid content, color difference, peroxide concentrations and volatile organic compounds (VOCs) of chili oil samples prepared from six kinds of edible vegetable oils (rapeseed oil, corn oil, soybean oil, olive oil, tomato seed oil, and camellia oil) were determined using high performance liquid chromatography (HPLC) and gas chromatography-ion mobility spectroscopy (GC-IMS). The six chili oil samples differed significantly in their capsaicinoid and peroxide concentrations and pungency (P<0.05) but had similar color difference values. GC-IMS analysis detected the presence of 88 VOCs, with aldehydes being the most predominant, comprising 22 different types. Orthogonal partial least squares-discriminant analysis screened out 19 key differential markers (VIP>1.2) for key flavors, among which 4-methylthiazole (VIP = 3.16) contributed the most to the flavor of the chili oil samples. The differences in VOCs among the six chili oil samples were effectively distinguished using GC-IMS fingerprint spectra combined with principal component analysis and heat map cluster analysis. The different vegetable oils had varying effects on the flavor and quality of chili oil, with rapeseed oil having a particularly prominent effect. Rapeseed-based chili oil was moderately pungent (17.72±0.12°), had a pure color and the richest aroma, and showed high stability. The findings of this study can serve as a theoretical basis for the production and identification of chili oil products with distinct flavors.
    37  Screening and Identification of A High Yield Antimicrobial Peptide F1-producing Strain and Biofilm Formation Inhibition in Multidrug-resistant Bacteria
    WANG Qun WU Jianuo YE Zhuming LIU Jingle WANG Juan YANG Zhijie CAO Yong
    2024, 40(10):351-361. DOI: 10.13982/j.mfst.1673-9078.2024.10.1183
    [Abstract](19) [HTML](11) [PDF 13.80 M](36)
    Abstract:
    Lactobacillus paracasei subsp. tolerans FX-6, as the original starting strain, was mutated through 60Co γ-ray irradiation. A strain (Strain 26) producing antimicrobial peptide F1 at high yield was identified from the mutated strains. High similarity between FX-6 and strain 26 was revealed through comparative analysis of their 16S rDNA sequences. The crude extract of milk fermented by Strain 26 was further purified to successfully enrich antimicrobial peptide F1. Bacteriostatic experiments showed that the minimum inhibitory concentration (MIC) of the crude extract of milk fermented by Strain 26 on Escherichia coli was 3.16 mg/mL, which was 75% lower than the MIC of the crudee xtract of milk fermented by FX-6, and the yield of antimicrobial peptide F1 by Strain 26 was 3.03-fold that by FX-6. The results preliminarily indicate a possible positive correlation between the concentration of antimicrobial peptide F1 and the antimicrobial activity of the strain. Our preliminary results have shown that the MIC of antimicrobial peptide F1 against colistin-resistant E. coli SHP45 was 320.0 μg/mL, and the results of this study further demonstrated that the biofilm formation in colistin-resistant E. coli SHP45 was inhibited by over 50% when the concentration of antimicrobial peptide F1 reached 2× MIC. These findings indicate that Strain 26 exhibited a stronger ability to produce antimicrobial peptide F1 than FX-6 and that antimicrobial peptide F1 significantly inhibited biofilm formation in colistin-resistant E. coli SHP45. This study offers insights into obtaining strains producing antimicrobial peptide F1 at high yield and is expected to serve as a material basis for the prevention and control of multidrug-resistant bacterial infections.
    38  Determination of Acibenzolar-S-methyl and Their Metabolite Acibenzolar Acid in Milk and Dairy Products by QuEChERS- Liquid Performance Chromatography-Tandem Mass Spectrometry
    WANG Chunming HUO Jianglian CHEN Dongdong PENG Tao YU Libo JIA Jingjian
    2024, 40(10):362-369. DOI: 10.13982/j.mfst.1673-9078.2024.10.0848
    [Abstract](15) [HTML](9) [PDF 2.10 M](28)
    Abstract:
    This study explored a test method for the determination of acibenzolar-S-methyl and acibenzolar acid in milk and dairy products by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The samples were subjected to vortex extraction with formic acid-acetonitrile (containing 1% (V/V) formic acid), and purified by QuEChERSdSPE EMR-Lipid tubes, centrifuged then filtered before detection. The test method used gradient elution using methanol-0.1% (V/V) formic acid as the mobile phase, a flow rate of 0.2 mL/min and ACQUITY UPLC HSS T3 column for separation. Electrospray positive and negative ion modes (ESI+ for acibenzolar-S-methyl and ESI- for acibenzolar) and multiple reaction monitoring (MRM) were used for detection, and the matrix-matched external standard method was used for quantification. For milk and dairy products, good linearity between the mass concentrations of acibenzolar-S-methyl and acibenzolar acid, and their corresponding peak areas, in the concentration range of 1~30 ng/mL, with the correlation coefficients R2 higher than 0.996. The mean recoveries of acibenzolar-S-methyl and acibenzolar acid were 91.8%~109.6% and the corresponding relative deviation (RSD) values were 1.3%~11.5% at three spiking levels (0.01, 0.02 and 0.1 mg/kg). This method can meet the requirements of accuracy, precision, and sensitivity for residue detection.
    39  Detection of Staphylococcus aureus Enterotoxins, Biofilm-forming Ability and Associated Genes in Pigeon Slaughtering and Processing
    DOU Tao LIU Yingyu WANG Jinquan MA Lan ZHANG Liuqing HU Yun
    2024, 40(10):370-378. DOI: 10.13982/j.mfst.1673-9078.2024.10.1145
    [Abstract](24) [HTML](18) [PDF 1.99 M](21)
    Abstract:
    To understand the enterotoxins, biofilm-forming ability and biofilm formation related genes of Staphylococcus aureus in pigeon slaughter and processing. The enterotoxin contents and biofilmforming abilities of 41 strains of Staphylococcus aureus were determined using the Staphylococcus aureus enterotoxin enzyme-linked immunoassay kit and crystal violet staining method. Meanwhile, 12 enterotoxin genes and 10 biofilm formation-related genes of Staphylococcus aureus were also amplified by PCR. Among the 41 strains of Staphylococcus aureus, 30 strains were enterotoxin-producing strains in enterotoxin enzyme-linked immunoassays. In the detection of enterotoxin genes, 35 strains of bacteria contained enterotoxin genes (with the detection rate being 85.36%), and have carried one or more enterotoxin genes. The detection rates of classical enterotoxin genes weresea (29.27%), seb (29.27%), sec (4.88%), sed (65.85%) and see (2.44%), respectively; The detection rates of novel enterotoxin genes were sel (36.59%), seg (29.27%), sem (17.07%), seu (17.07%), sei (14.63%), she (7.32%) and sek (0.00%), respectively. The detection rates of the strains with weak, moderate and strong biofilm-forming abilities determined by crystalline violet staining were 39.02%, 43.90% and 17.07%, respectively. In the detection of biofilm formation related genes, the detection rates of clfB, luxS and clfA were the highest (all at 100.00%), followed by sarA and ccp (both at 97.56%); the detection rates of sigB, cna, icaA and agrC were 85.37%, 65.85%, 41.46% and 2.44%, respectively, and bap was not detected. In addition, all strains were found to carry multiple biofilm-related genes, and the number of the strains carrying 7 genes was the highest (15/41, 36.59%). The Staphylococcus aureus contaminated in the pigeon slaughtering and processing had more enterotoxins and higher biofilm-forming ability. It was predicted that the Staphylococcus aureus found in the pigeon slaughter and processing was pathogenic and had potential risk of causing food poisoning.
    40  Determination of 15 Organophosphate Esters in Meat Products by Dispersive Solid Phase Extraction-gas Chromatography-triple Quadrupole Tandem Mass Spectrometry
    LI Haixia CHEN Keyun JU Xiang LIANG Xiuqing TIAN Qiyan LI Jie LI Xia WANG Yanli CUI Yuhua LIU Yanming
    2024, 40(10):379-388. DOI: 10.13982/j.mfst.1673-9078.2024.10.1096
    [Abstract](17) [HTML](11) [PDF 2.96 M](26)
    Abstract:
    In this study, an analytical method for the determination of 15 organophosphate esters (OPEs) in meat products was established based on dispersive solid phase extraction-gas chromatography-triple quadrupole tandem mass spectrometry (GC-MS/MS). The organophosphates in the sample was extracted with acetonitrile, purified with enhanced matrix removal adsorbent (enhanced matrix removal-lipid, EMR-Lipid), separated by HP-5MS column, determined by GC-MS/MS dynamic multi-reaction ion monitoring (dMRM) mode, and quantified by the matrix matching external standard method. The results showed that chromatographic separation and analysis of 15 OPEs were completed within 21 min, and good linear relationship of the target substances was obtained in the range of 0.01~100 ng/mL, with the correlation coefficients greater than 0.999. The average spike recoveries of 15 kinds of organic phosphate esters at different spiking levels (1.25, 12.5 and 25 ng/g) were 72.8%~123.8%, with the relative standard deviations (RSDs) (n=6) being1.6%~14.7%, the detection limit (LOD) of the method being 0.25~0.40 ng/g and the limit of quantification (LOQ) being 0.80~1.25 ng/g. The method was applied to 70 commercial meat products samples. The results showed that 7 organophosphate esters were detected in 58 samples with a detection rate of 82.8%. Among which, the three organophosphate esters with higher detection rates were tri (2-chloro-1-(chloromethyl) ethyl) phosphate, tri (2-chloro-ethyl) phosphate and triphenyl phosphate. The method established in this study has the advantages of simple operation, high speed and good purification effect, and can be used for the rapid detection of 15 organophosphate esters in meat products.
    41  Research Progress on the Application of Hydrogen Nanobubble Water
    TIAN Yu WAN Xiaobao SONG Ke WANG Junhao HAO Haihong
    2024, 40(10):389-397. DOI: 10.13982/j.mfst.1673-9078.2024.10.1046
    [Abstract](31) [HTML](15) [PDF 5.08 M](29)
    Abstract:
    Hydrogen is a colorless, odorless, water-insoluble and highly flammable diatomic gas that can act as a reducing agent in certain circumstances. A large number of studies have shown that hydrogen has potential therapeutic effects on more than 100 kinds of diseases, such as metabolic diseases, neurodegenerative diseases, ischemia reperfusion injury and tumors. However, hydrogen not only has low solubility in water but also has a short residence time, which is the main factor limiting the widespread application of hydrogen-rich water. However, due to their high internal pressure and negative surface charge, nanobubbles can increase the solubility and residence time of hydrogen in water, and enhance their antioxidant capacity. The development of nanobubble technology has also promoted the research, development and application of hydrogen nanobubble water by domestic and international scholars. This article mainly summarizes the latest research progress of hydrogen nanobubble water, in order to provide a reference for future application research of hydrogen nanobubble water.
    42  Progress of Research on the Anti-inflammatory Activity of Dietary Polyphenols and the Mechanisms whereby They Affect Inflammatory Signaling Pathways
    ZHAO Juyang YAO Hengzhe LI Yuqi LI Yue WANG Pengyu
    2024, 40(10):398-408. DOI: 10.13982/j.mfst.1673-9078.2024.10.0603
    [Abstract](21) [HTML](9) [PDF 2.64 M](30)
    Abstract:
    Fruits, vegetables, grains, spices, and herbs are rich sources of dietary polyphenols. These polyphenols are closely associated with the nutritional value and biological activity of plants. Polyphenols can interact with polysaccharides, proteins, phospholipids, and other macromolecular substances and affect their activity and bioavailability. Inflammation is a defense response of the body to stimuli; excessive inflammatory responses can easily harm the human body. Polyphenols exert anti-inflammatory effects by scavenging oxygen free radicals, changing the redox state of cells, preventing lipid peroxidation, inhibiting neutrophil extravasation, and suppressing the production of inflammatory factors. Dietary polyphenols are predominantly found in vegetables, nuts, tea, and cocoa. In recent years, the mechanism whereby polyphenols mediate the occurrence of related inflammatory diseases by binding with various receptors has become a research hotspot. This paper summarizes the positive effects of dietary polyphenols on the treatment of inflammatory diseases, the anti-inflammatory activity of dietary polyphenols, the molecular mechanism underlying their involvement in inflammatory responses, the specific receptors and binding sites of different polyphenols, and the inflammatory signaling pathways affected by polyphenols. The findings of the present study will provide insights for further research on the mechanisms underlying the anti-inflammatory activity of polyphenols and a theoretical basis for the development of polyphenol-rich functional foods.

    Editor in chief:李琳

    Inauguration:现代食品科技

    International standard number:ISSN 1673-9078

    Unified domestic issue:CN 44-1620/TS

    Domestic postal code:46-349

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