Volume 40,Issue 1,2024 Table of Contents

  • Display Type:
  • Text List
  • Abstract List
  • 1  Effects of Black Soybean-whey Protein Blend on Immune Regulation in Rats
    LIANG Defu MENG Weihong SHU Xin ZHUANG Kejin ZHANG Dongjie
    2024, 40(1):1-9. DOI: 10.13982/j.mfst.1673-9078.2024.1.1601
    [Abstract](176) [HTML](121) [PDF 3.48 M](441)
    Abstract:
    Black soybean protein and whey protein are two kinds of high-quality plant or animal protein materials. Their consumption can improve the immune characteristics of the body. However, there are relatively few studies on the immunomodulatory effect of the black soybean-whey protein blend. In this study, four groups of rats were fed with a standard diet (CP), whey protein diet (WP), black bean protein diet (BSP) and black soybean-whey protein blend diet (BS-WP) for 60 days. At the end of the feeding cycle, serum biochemical indicators, IgM, IgA and IgG mass concentrations, spleen lymphocyte proliferation index, natural killer cell activity, spleen index and thymus index were measured to explore the regulatory effect of black soybean-whey protein blend on the immune system of rats. Results showed that compared with the single protein source group, the serum immune biochemical indexes (total protein, albumin, globulin, urea nitrogen, alanine transaminase levels) of the black soybean-whey protein blend group significantly increased (P<0.05). The mass concentrations of IgG, IgA and IgM increased significantly (P<0.05), with the increase of IgG content being the most significant. Compared with the groups treated whey protein or black soybean protein singly, the IgG content of the protein blend group increased by 38.23% and 41.02%, respectively. In addition, the spleen and thymus indexes significantly increased (P<0.05), with the increase of the spleen index being 35.33% and 35.91%, respectively, and the increase of the thymus index being 31.51% and 34.26%, respectively. Therefore, black soybean-whey blended protein blend diet can significantly improve the body immunity capacities of rats.
    2  Shanxi Mature Vinegar Attenuates Liver Injury Induced by a High-fat Diet in Rats through the JAK2/STAT3 Pathway
    LIU Jiajia SHEN Tong CHEN Jia PANG Quanhai
    2024, 40(1):10-18. DOI: 10.13982/j.mfst.1673-9078.2024.1.0096
    [Abstract](111) [HTML](111) [PDF 4.73 M](323)
    Abstract:
    A hyperlipidemia rat model was used to explore the mechanism by which Shanxi aged vinegar reduces liver injury induced by a high-fat diet. The model involved intragastric administration of high-fat emulsion to rats for 5 weeks, whereas low, medium, and high doses (1.35, 2.7, 5.4 g/kg by body weight) of Shanxi aged vinegar were administered to the rats by gavage for 10 weeks. Serum total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), superoxide dismutase (SOD), glutathione reductase (GRx), total antioxidant capacity (T-AOC), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALK-P) levels were determined by serological testing. Pathological changes in the rat livers were examined using hematoxylin and eosin (H&E) staining. The mRNA and protein levels of JAK2 and STAT3 in the livers were detected using q-PCR and WB and immunohistochemical techniques were used for localization analysis.The results showed that Shanxi aged vinegar significantly reduced the serum lipid content and extent of liver injury in rats fed a high-fat diet (P<0.01), increased the activity of antioxidant enzymes, and significantly inhibited mRNA and protein levels of JAK2 and STAT3 in the rats (P<0.01). More specifically, serum TC and TG in the high-dose group was decreased by 20.50% and 48.65%, respectively, compared to the low-dose group. The high-dose and medium-dose groups showed the largest effects on AST (32.79% and 29.07%), ALT (21.65% and 20.67%) and ALK-P (19.42% and 19.71%). Compared with the low-dose group, the high-dose group had significantly lower mRNA levels of JAK2 (57.07%, 34.02%) and STAT3 (55.19%, 41.45%), and significantly lower protein levels of JAK2 (27.89%, 24.09%) and STAT3 (33.06%, 26.64%) (P<0.05, P<0.01, respectively). In conclusion, Shanxi aged vinegar attenuated liver injury induced by a high-fat diet in rats, and this may be achieved through inhibition of activation of the JAK2/STAT3 pathway.
    3  Anti-obesity and Lipid-lowering Effects of Ellagic Acid and Fisetin from Yunnan’s Wild Phyllanthus emblica
    LIN Qianru HU Hai’e LI Xuel MA Ruiting XU Lu LIU Guo DENG Xiwe HOU Tao KAN Qixin CHEN Yuli CAO Yong
    2024, 40(1):19-26. DOI: 10.13982/j.mfst.1673-9078.2024.1.1193
    [Abstract](140) [HTML](102) [PDF 9.12 M](372)
    Abstract:
    Phyllanthus emblica (PE) is an edible and medicinal fruit that has received much attention in recent years, but little has been reported about its anti-obesity and lipid-lowering effects as well as specific functional components. In this study, the flavonol-rich polyphenols from Yunnan wild PE were extracted (Phyllanthus emblica Extract, PEE). It was found that in the 3T3-L1 cell model, PEE could significantly inhibit the differentiation of 3T3-L1 cells into mature adipocytes and reduce the production of lipid droplets. When PEE at 300 μg/mL was applied, the relative contents of total triglyceride (TG) and total cholesterol (TC) in cells were reduced to 66.47% and 40.87%, respectively, suggesting that PEE in Yunnan wild PE has the potential to promote weight loss and lipid reduction. HPLC analysis revealed that Yunnan wild PE contained ellagic acid (3 030.78 μg/g) and fisetin (560.70 μg/g). Ellagic acid and fisetin were mixed at a 5:1 ratio to form a mixture (EF), and the effect of EF on a high-fat diet (HFD) mouse model was examined. The results showed that EF could inhibit the growth of body weight and white fat in HFD mice, and decreased the serum TG, TC and LDL-C contents by 45.48%, 12.06% and 37.54%, respectively, while increasing the HDL-C level by 18.75%. The liver index, serum ALT and AST of the HFD mice decreased by 7.80%, 21.59% and 41.64%, respectively. EF exhibited anti-obesity and lipid-lowering effects, and could exert certain repairing functions on the liver, thus, EF are important components accounting at least paritially for the anti-obesity and lipid-lowering effects of Yunnan wild PE.
    4  Regulatory Effects of Ginsenoside F2 on LPS-induced Inflammation in RAW264.7 Cells
    NIU Zhiqiang LI Qi LIU Yanan HU Yeye HE Ziliang HU Weicheng TAO Yongxia ZHANG Ji
    2024, 40(1):27-33. DOI: 10.13982/j.mfst.1673-9078.2024.1.0141
    [Abstract](127) [HTML](115) [PDF 4.06 M](463)
    Abstract:
    Most ginsenosides (the main bioactive substances in ginseng) have been shown to exert potent anti- inflammatory effects, but their anti-inflammatory mechanisms, especially that of ginsenoside F2 (GF2), are incompletely understood. This study was conducted to explore the anti-inflammatory effect of GF2 in an in vitro model of inflammation using LPS-induced RAW264.7 cells. Cell viability analysis revealed that the safe concentration threshold of GF2 to be 100 μmol/L. GF2 significantly inhibited NO release in a dose-dependent manner (0~100 μmol/L) in LPS-induced RAW264.7 cells. GF2 (50, 100 μmol/L) also inhibited the mRNA expression of iNOS, IL-1β, COX2, and TNF-α. GF2 markedly downregulated the protein expression of iNOS, but not that of COX2. Further, treatment with 100 μmol/L GF2 markedly attenuated LPS-induced cell morphology changes in RAW264.7 cells. A western blot showed that GF2 increased PDK1, AKT, and IκB-α protein expression, and decreased AKT phosphorylationand NF-κB nuclear translocation. Our findings demonstrate that GF2 exerts potent anti-inflammatory effects and suggest that these effects are achieved through the AKT/IκB-α/NF-κB signaling pathway. This study can thus provide theoretical support for the development of natural anti-inflammatory drugs.
    5  Effects of Bifidobacterium animalis and Limosilactobacillus reuteri on Fat Distribution and Blood Fat in Obese Mice
    ZHU Meizhen ZHENG Hanying GUO Dongbei CHEN Xiaoxuan PAN Lili LI Hongwei
    2024, 40(1):34-46. DOI: 10.13982/j.mfst.1673-9078.2024.1.1543
    [Abstract](82) [HTML](131) [PDF 4.23 M](373)
    Abstract:
    To explore the effects of Bifidobacterium animalis CP-9 (CP-9) and Limosilactobacillus reuteri ADR-1 (ADR-1) on weight, fat distribution and blood fatin obese mice. The obese mice fed with high fat diet were randomly divided into model control group (High-fat Diet, HFD), CP-9 group, ADR-1 group. The mice in blank control group (Normal Diet, ND) were fed with normal diet. The body weight and body composition of mice were measured, the distribution of body fat and the pathological changes of liver tissue were observed, the blood biochemical indexes were measured, and the protein expressions of fatty acid synthase (FAS) and peroxisome proliferator-activated receptor γ (PPARγ) in liver tissue were determined. At the end of the intervention, compared with HFD, the body weight (-18.82%, -18.82% vs HFD), abdominal and subcutaneous fat, serum low-density lipoprotein cholesterol (LDL-C), triglyceride (TG) and alanine aminotransferase (ALT) of mice in theCP-9 and ADR-1 groups decreased significantly (P<0.05). Liver steatosis and liver injury in CP-9 and ADR-1 groups were significantly improved compared with HFD group, and the protein expression of FAS gene in liver decreased (-53.22%, -53.22% vs HFD), while the protein expression of PPAR γ gene increased (571.43% vs HFD 714.29%). In summary, the intervention of CP-9 andADR-1 can effectively reduce the body weight, abdominal and subcutaneous fat of obese mice, improve hepatic steatosis and liver injury, probably by regulating the protein expression of FAS and PPAR γ genes in liver to reduce TG and LDL-C so as to improve the abnormality of fat metabolism.
    6  Protective Effect of a Complex of Blueberry, Blackcurrant, Medlar and Cassia Obtusifolia against Oxidative Stress Injury in Retinal Epithelial Cells
    WANG Huan WANG Min LIU Zhuqing HE Yunhui SHI Juran ZOU Shengcan
    2024, 40(1):47-53. DOI: 10.13982/j.mfst.1673-9078.2024.1.1613
    [Abstract](79) [HTML](104) [PDF 4.82 M](342)
    Abstract:
    To study the protective effect of blueberry, blackcurrant, medlar, cassia seed complex (LHGJ) against the oxidative stress injury in human retinal epithelial cells (APRE-19) induced by methylglyoxal (MGO). The ARPE-19 cell model was established through inducing oxidative stress by MGO, and LHGJ solutions at different concentrations were used to treat the cells. Cell viability was determined by the CCK-8 method, the reactive oxygen species (ROS) content in cells was measured by the fluorescence microscopy method, the nitric oxide (NO) content was measured by enzyme-linked immunosorbent assay (ELISA), the glutathione peroxidase (GSH-PX) content was measured by colorimetry, and the superoxide dismutase (SOD) activity was measured by the hydroxylamine method. The results showed that oxidative stress injury in ARPE-19 cells could be induced by 0.80 mmol/L MGO. The induced ARPE-19 cells were then treated with 0.10 or 1.00 μg/mL LHGJ complex, which increased the cell viability by 23.03% and 21.23%, respectively (P<0.001). LHGJ complex solutions at 0.01, 0.10 and 10.00 μg/mL significantly (P<0.05) reduced the fluorescence intensity of ROS (by 1.02, 1.28 and 2.37 times, respectively), LHGJ complex solutions at 0.01, 0.10 and 10.00 μg/mL significantly (P<0.05) decreased NO production (by 13.24, 15.23 and 16.24 μmol/g prot., respectively), LHGJ complex solutions at 1.0 and 10.00 μg/mL significantly (P<0.05) increased the activity of SOD (by 6.01 and 7.43 U/mg, respectively) (P<0.05, P<0.01) and the content of GSH-Px (by 14.32 and 25.42 U/mg, respectively) (P<0.05). LHGJ complex can protect ARPE-19 cells against oxidative stress injury induced by MGO, which provides a theoretical basis for further development of LHGJ complex.
    7  Comparison of the Antioxidant Activities of Fermented Pu-erh Teas with Monascus and/or Lactobacillus plantarum
    HUANG Yuan YU Juan JIANG Xiaoli REN Ling DONG Rui LU Qian WANG Jianfei LI Yali ZHOU Hongjie
    2024, 40(1):54-60. DOI: 10.13982/j.mfst.1673-9078.2024.1.0070
    [Abstract](78) [HTML](123) [PDF 1.89 M](349)
    Abstract:
    The effects of the fermentation with Monascus and/or Lactobacillus plantarum on the biochemical components and antioxidant activities of Pu-erh teas were investigated. The correlation coefficient method was used to analyze the correlation between the main biochemical components and antioxidant indexes. The experiments showed that the contents of water extract, flavonoids and tea polyphenols of the six fermented tea samples were in the range of 50.64%~52.50%, 0.77~0.95 mg/g and 13.21%~11.07%, respectively, with the tea fermented with L.plantarum alone (R) having the highest tea polyphenol content (13.21%). There were significant differences (P<0.05) in the in vitro antioxidant capacity of the tea samples subjected to different treatments. R had the highest total antioxidant capacity, DPPH scavenging rate and hydroxyl radical scavenging rate (10.36 μg Trolox/mL, 91.59%, 29.43%, respectively). The correlation analysis showed that theophyllin and theaflavin were negatively correlated with antioxidant capacity (though not significantly); caffeine, tea polyphenols, theaflavin and total flavonoid indexes were significantly correlated with antioxidant capacity, among which tea polyphenols were highly significantly correlated with total antioxidant capacity and DPPH radical scavenging rate (P<0.01) with the correlation coefficients being 0.74 and 0.95, respectively. The Pu-erh teas fermented with added strains had a relatively strong antioxidant capacity, and the antioxidant capacity of the fermented Pu-erh tea with a single strain was stronger than that with mixed strains, with the R having the highest antioxidant capacity.
    8  Gallic Acid Both Inhibits and Promotes the Growth of Lactobacillus plantarum
    LIU Xinning LU Yunhao HE Qiang
    2024, 40(1):61-66. DOI: 10.13982/j.mfst.1673-9078.2024.1.0164
    [Abstract](95) [HTML](131) [PDF 2.08 M](412)
    Abstract:
    The effects of gallic acid (GA) and epigallocatechin gallate (EGCG) on the growth of Lactobacillus plantarum CICC 6253 were investigated by monitoring the microbial growth curves, concentration changes of the polyphenols, and pH changes of the culture media. GA and EGCG each exhibited two-way regulatory effects on L. plantarum, both inhibiting and promoting the growth of the bacterium in a concentration-dependent manner, and there were differences in structure–activity relationship between the two effects. L. plantarum growth was inhibited at GA and EGCG concentrations above 5 mg/mL and 7 mg/mL, respectively. In contrast, the growth of the bacterium was promoted at GA and EGCG concentrations below 4 mg/mL and 6 mg/mL, respectively, with the effect increasing with increasing concentrations of the polyphenols. GA served as a carbon source that could be rapidly utilized by L. plantarum, whereas EGCG (4 mg/mL) effectively promoted the proliferation of the strain owing to its good cell affinity. The total number of colonies on the EGCG- containing medium increased from 7.16 lg CFU/mL to 12.54 lg CFU/mL in 28 h, which was an increase of more than two orders of magnitude compared with the control group. Both GA and EGCG showed two-way regulatory effects on L. plantarum, but EGCG exhibited superior prebiotic properties. The results of this study can serve as a reference for the use of GA, EGCG, and similar food-derived polyphenols in regulating the growth of probiotics and for the research and development of related foods.
    9  Screening of Lactic Acid Bacteria in Traditional Fermented Meat Products and Their Application in Salami Fermentation
    WANG Chunyu XU Baocai YANG Liu SUN Yun
    2024, 40(1):67-75. DOI: 10.13982/j.mfst.1673-9078.2024.1.0181
    [Abstract](91) [HTML](107) [PDF 5.59 M](388)
    Abstract:
    To screen for strains of lactic acid bacteria with excellent fermentation characteristics, the microorganisms in traditional fermented meat products were selectively isolated and their adaptability in salami fermentation was investigated. Through the analysis of the fermentation characteristics of different strains, two strains YR07 and L.48 with superior fermentation performance were selected on the basis of several criteria, including an absence of viscosity and pigment production, H2O2 and H2S production, gas production during fermentation, amino acid decarboxylase and hemolysis, and arginine decomposition to produce ammonia. Based on the sugar fermentation test and 16S rRNA sequence analysis, the strain YR07 was identified as Lactiplantibacillus plantarum and L.48 as Latilactobacillus sakei. Strains YR07 and L.48 showed good tolerance to 6.0% NaCl, 150 mg/kg NaNO2, and pH 4.0. These strains were also able to effectively inhibit food- borne pathogenic and spoilage bacteria, including Staphylococcus aureus, Escherichia coli, and Salmonella typhimurium, and showed no resistance to common antibiotics, including erythromycin and chloramphenicol. The two strains were applied separately to salami fermentation. The pH of the salami in the inoculated group was rapidly reduced to 4.71 and 4.82, respectively, within 24 h; the growth of miscellaneous bacteria was inhibited; and the sensory scores for texture and flavor of the product were improved. In conclusion, YR07 and L.48 meet the requirements for starter cultures, exhibiting strong production adaptability and high safety, and hence can be used for meat fermentation.
    10  Screening of Lactic Acid Bacteria to Improve the Fermentation and Antioxidant Properties of Bean Milk
    WANG Jingli MENG Jun ZHU Mengxi ZHANG Shaobing
    2024, 40(1):76-83. DOI: 10.13982/j.mfst.1673-9078.2024.1.0119
    [Abstract](89) [HTML](133) [PDF 5.87 M](468)
    Abstract:
    Seventy-four strains of lactic acid bacteria were screened from traditional fermented foods and healthy human intestines through isolation and purification, morphological observation, and 16S rDNA identification. Five strains of these bacteria (Lactiplantibacillus plantarum Q2.3, L. fermentum LF3.2, L. pentosus Q1.4, L. plantarum G1.4, L. plantarum G1.3) with optimal gastrointestinal fluid tolerance, acid production capacity, and proliferation capacity were identified. Effectiveness of the five bacterial strains in the fermentation of soy milk, peanut milk, and chickpea milk, was examined. L. fermentum LF3.2 produced the most acid in the three kinds of bean milk after 24 h of fermentation, with minimum pH values lower than 4.5 for the fermented soy milk and peanut milk. At this point, the viable bacteria count in all three fermented bean milks exceeded 8 log CFU/mL. L. fermentum LF3.2 and L. plantarum G1.3 proliferated most in soy milk and peanut milk, while L. fermentum LF3.2 and L. plantarum G1.4 proliferated most in chickpea milk. The antioxidant activities of all three bean milks were significantly increased after 24 h of fermentation by L. fermentum LF3.2 and L. plantarum G1.3. Scavenging of α, α-Diphenyl-β-Picrylhydrazyl free radicals (DPPH.) and hydrogen radicals (OH.) were 23.34% and 23.64% higher, respectively, in fermented soy milk than in unfermented soy milk. L. fermentum LF3.2 increased the DPPH. clearance rate and L. plantarum G1.3 increased the OH. clearance rate of all three bean milks. These findings serve as guidance for the development of functional fermented bean milk, and lay a theoretical foundation for the application of lactic acid bacteria in plant-based fermented foods.
    11  GC-MS Analysis of the Composition of Three Citrus Essential Oils and Comparison of Their Microbiostatic Activities
    CHEN Yan PAN Xiaowei TAO Hong LIN Lianzhu
    2024, 40(1):84-91. DOI: 10.13982/j.mfst.1673-9078.2024.1.0170
    [Abstract](131) [HTML](150) [PDF 7.12 M](443)
    Abstract:
    Three citrus species, Citri Reticulatae Pericarpium (CRP), lemon and kumquat, were selected as raw materials for the extraction of essential oils by supercritical CO2 extraction. Gas chromatography-mass spectrometry (GC-MS) was used to analyze the components of the samples, and the microbiostatic activities of the three essential oils against Staphylococcus aureus and Aspergillus fumigatus were also studied. The results showed that lemon essential oil had relatively high levels of alcohols (3.41%), aldehydes (2.55%), ketones (2.03%), esters (7.17%) and terpenes (34.06%), whereas the content of D-limonene (46.52%) was the lowest among the three essential oils. CRP essential oil had relatively high levels of terpenes (86.34%) and phenolic compounds (1.82%), while kumquat essential oil had relatively high levels of acid compounds (12.71%) and D-limonene (72.69%). The microbiostatic activities of these essential oils against Staphylococcus aureus and Aspergillus fumigatus were ranked as follows: lemon essential oil>CRP essential oil>kumquat essential oil. D-limonene was not the only microbiostatic component in the three essential oils, and their microbiostatic activities were closely related to microbiostatic components other than D-limonene. Other microbiostatic components, including γ-pinene and β-pinene, which were abundant in lemon essential oil, can work synergistically with D-limonene to effectively improve its microbiostatic activity. Essential oils extracted by this method can help to facilitate the development of related natural microbiostatic products.
    12  Optimization of Inducer Conditions for Promoting Triterpene Production in Liquid State Fermentation of Morchella
    ZHU Yongle GAO Mengxiang LI Li ZHANG Jialan
    2024, 40(1):92-101. DOI: 10.13982/j.mfst.1673-9078.2024.1.0145
    [Abstract](84) [HTML](121) [PDF 4.61 M](380)
    Abstract:
    To fully utilize Morchella resources and increase its triterpene content, the optimal conditions of multiple inducers for promoting triterpene production in liquid fermentation of the fungus were determined using single-factor experiments and response surface methodology. Additionally, the activities of two key enzymes in the triterpene biosynthetic pathway were measured. According to the results obtained, the optimal treatment conditions were a magnetic field intensity of 2.81 mT, gibberellin concentration of 0.56 g/L, and salicylic acid concentration of 214.54 mmol/L. Under these conditions, the maximum concentration of triterpenes accumulated in Morchella was 60.26 mg/g, which was 160.72% (m/m) more than that before optimization. The enzymatic activities of squalene synthase and farnesyl pyrophosphate synthetase were 39.93 and 71.08 IU/L, respectively, which were 190.85% and 178.02% (m/m) (SS) more than that of the control group (FPS). Therefore, combined treatment with a magnetic field, gibberellins, and salicylic acid can considerably promote triterpene production by Morchella. Furthermore, the accumulation of triterpenes was attributed to the enhanced activity of two key enzymes in the triterpene biosynthetic pathway. This study provides a theoretical basis for the development of inducers for enriching beneficial metabolites and new products of Morchella.
    13  Comparison of the Physicochemical Properties and Functional Properties of Insoluble Dietary Fiber from Three Food Sources
    ZHANG Xinyue ZHANG Min DENG Mei DONG Lihong MA Qin JIA Xuchao CHEN Yanxia ZHANG Ruifen
    2024, 40(1):102-111. DOI: 10.13982/j.mfst.1673-9078.2024.1.011
    [Abstract](130) [HTML](119) [PDF 3.93 M](459)
    Abstract:
    To explore differences in adsorption capacity among dietary fiber from different sources, insoluble dietary fiber (IDF) from three different sources (pomelo, carrot, and rice bran) were selected for comparative analysis of their microstructural characteristics, physiochemical properties (including water and oil holding capacities, swelling capacity, and viscosity) and adsorption properties (including cholate adsorption capacity, diffusion kinetics of cholate release, cholesterol, glucose and nitrite absorption capacities). Carrot IDF was composed of smooth spherical particles and exhibited the strongest swelling capacity (7.92 mL/g), and cholate (57.80 mg/g) and cholesterol (11.72 mg/g) adsorption capacities in a simulated intestinal environment (pH valu 7) among the three IDFs. Rice bran IDF had a rough, porous and irregular structure and exhibited the strongest oil holding capacity (3.00 g/g), glucose adsorption capacity (20.67 mmol/g), nitrite absorption capacity (1 482.22 μg/g at pH 7 and 2 836.23 μg/g at pH valu 2), cholic acid blocking capacity (4.47 mg/g) and cholesterol adsorption capacity (8.56 mg/g) in a simulated gastric environment (pH valu 2). Pomelo IDF had a layered scale-like structure and exhibited the strongest water holding capacity (15.48 g/g), high viscosity, and excellent cholic acid blocking capacity (3.55 mg/g). The three dietary fibers from different sources had their own microstructural and physicochemical characteristics, which resulted in different adsorption properties. This study provided data support for the processing and utilization of different types of dietary fiber in health foods.
    14  Analysis of the Physicochemical Properties, Cooking and Eating Quality of Selenium-enriched Rice and Ordinary Rice
    XU Rui LI Shuyi MEI Xin ZHU Zhenzhou ZHOU Lei SHI Jianbin CAI Sha XIONG Tian CAI Fang SUI Yong
    2024, 40(1):112-120. DOI: 10.13982/j.mfst.1673-9078.2024.1.0102
    [Abstract](71) [HTML](119) [PDF 2.97 M](345)
    Abstract:
    Selenium-enriched rice is an important source of selenium supplement for humans in daily life. Four indica rice varieties (Juliangyou 60, Yexiangyouhang 1573, Xiadao No.1, and Huaxiaxiangsi) and two japonica rice varieties (Nanjing 5055 and Nanjing 9108) were selected as experimental materials to explore the effects of selenium enrichment on their physicochemical properties, and cooking and eating quality. Selenium enrichment promoted protein accumulation in rice ~3 wt% ~16 wt%, compared with ordinary rice. Selenium had no significant effect on the gelatinization, texture, thermal properties and cooking quality of rice, but significantly reduced eating quality (P<0.05). Correlation analysis showed that the correlation coefficients of selenium content with protein, ash, rice hardness, and the pH of rice soup were 0.54 and 0.46, 0.48, and -0.55, respectively. There was no correlation with other physicochemical indices. There was a negative correlation between selenium content and rice appearance, mouthfeel, and taste values; the correlation coefficients were -0.62, -0.73 and -0.79, respectively. Compared with ordinary rice, selenium-enriched rice had higher contents of total selenium, protein, and inorganic substances, which enhanced its nutritional quality, but its eating quality was reduced. This study can serve as a reference for research concerning the quality and processing of selenium-enriched rice.
    15  Effects of Protein Oxidation at Different pH values on the Gelling Properties of Myofer Fibroin in Yak Muscle
    QU Sha HU Ting TANG Shanhu LI Sining HAO Gang
    2024, 40(1):121-127. DOI: 10.13982/j.mfst.1673-9078.2024.1.1452
    [Abstract](95) [HTML](117) [PDF 4.19 M](332)
    Abstract:
    In this study, the gels formed by yak muscle myofibril protein (MP) incubated for 24 h in Fenton oxidation systems at different pH values were taken as the research objects. Through examining their characteristics such as whiteness, water retention and texture, the effects of protein oxidation on the gel propertiesat different pH values were explored. The differences in chemical force, rheology and microstructure under the conditions were analyzed. The results showed that the whiteness, water-holding capacity and texture of protein gel decreased significantly with an increase of H2O2 concentration at different pH values (P<0.05). The hydrophobic interaction force was the main force to maintain the protein gel, followed by disulfide bond, ionic bond and hydrogen bond. With the increase of H2O2 concentration, the hydrophobic interaction force and disulfide bond increase significantly, whilst the ionic bond and hydrogen bond decrease significantly (P<0.05). When the pH value was 5.0, oxidation had the most obvious effect on the gel characteristics. MP gel had the lowest water holding capacity (77.20%), the worst texture strength (only 53.40 g), the smallest storage modulus, and the weakest intermolecular force between proteins, leading to a loose and disordered microstructure. When pH value was 6.0, oxidation had the weakest effect on gel characteristics. MP gel had the highest texture strength (61.40 g), the largest storage modulus and intermolecular force, with the microstructure being compact and orderly. This study provides a theoretical basis for the control of protein oxidation in yak meat.
    16  Inhibitory Effect of p-Anisaldehyde Against Aspergillus flavus Growth
    XIE Yijia LI Xiaofeng YU Yigang ZHANG Ziqiang LIN Yihan XIAO Xinglong
    2024, 40(1):128-136. DOI: 10.13982/j.mfst.1673-9078.2024.1.0234
    [Abstract](106) [HTML](113) [PDF 5.92 M](356)
    Abstract:
    The antifungal activity of p-anisaldehyde (AS) against Aspergillus flavus was determined using the two-fold dilution method in 96-well plates. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of AS against A. flavus were found to be 0.80 mg/mL and 3.20 mg/mL, respectively. Spore germination, mycelial biomass synthesis, radial growth, and aflatoxin B1 (AFB1) synthesis were all completely inhibited in A. flavus cultures treated with 2 MIC (1.60 mg/mL) AS. Compared with the control group, the 4 MIC AS group released considerably more intracellular nucleic acid (A260) and protein (A280) within 24 h, as well as exhibited increased extracellular conductivity and pH value. The results of calcofluor white (CW) staining showed that AS treatment had no substantial effect on the cell wall septum of A. flavus. Moreover, 2 MIC AS could effectively inhibit the growth of A. flavus on pistachios, and the total number of molds decreased by 1.89 lg CFU/g on the 8th day. In conclusion, AS treatment substantially inhibited the growth of A. flavus and reduced the mold on pistachios. Therefore, it is expected to be developed as a new fungistatic agent for the food industry.
    17  Evaluation of Quality Changes in Freshwater Fish during Storage Using Electronic Nose Measurements and Biogenic Amine Contents
    LIU Lin JIAO Wenjuan ZHAO Tiantian ZHANG Yousheng ZHOU Fang NAN Haijun ZHANG Yehui HUANG Wen
    2024, 40(1):137-148. DOI: 10.13982/j.mfst.1673-9078.2024.1.0180
    [Abstract](94) [HTML](108) [PDF 2.82 M](392)
    Abstract:
    The effects of storage temperature and duration on the freshness of three kinds of freshwater fish (grass carp, tilapia, and bighead carp) were analyzed. The changes in several physicochemical indicators [including color, pH, and thiobarbituric acid reactive substances (TBARS) and total volatile basic nitrogen (TVB-N) contents] during storage at 25 ℃ and 4 ℃ were monitored, and sample freshness was evaluated on the basis of electronic nose measurements and biogenic amine (BA) contents. The results showed that with increasing storage time at 25 ℃ and 4 ℃, the b* and total chromatic aberration (ΔE) values and BA, TVB-N, and TBARS contents gradually increased, whereas the L* and a* values decreased, and the pH value first decreased and then increased. The TVB-N amount in all three types of fish exceeded 15 mg N/100 g after storage at 25 ℃ for 12 h and 4 ℃ for 6 days. Putrescine and cadaverine were detected, indicating that the fish had begun to deteriorate. Notably, the electronic nose response signals could accurately distinguish the freshness of all fish samples. In conclusion, storage temperature and time are key factors influencing the freshness of freshwater fish, the quality changes of which can be accurately determined by evaluating characteristic BAs and electronic nose response signals. In addition to verifying the feasibility of these evaluation methods, the results provide a theoretical basis for the freshness evaluation of freshwater fish products.
    18  Quality Changes of Pre-made Chicken Chops under Different Liquid Nitrogen Flash-freezing Temperatures
    HUANG Wenquan KAN Qixin HUANG Fengjing LI Yukun YANG Han LIU Guo CHEN Yuli XIE Canjie SONG Mingyue
    2024, 40(1):149-157. DOI: 10.13982/j.mfst.1673-9078.2024.1.0110
    [Abstract](106) [HTML](117) [PDF 3.83 M](400)
    Abstract:
    To explore the quality changes of pre-made chicken chops under different liquid nitrogen flash-freezing temperatures, liquid nitrogen spray (-80, -100, -120 ℃) was used to freeze chicken chops, whereas fresh storage at 4 ℃ and slow freezing at 18 ℃ were used as controls. The evaluation indicators included freezing speed, sensory score, water holding capacity, pH, color, texture and oxidation index. The results showed that treating chicken chops with liquid nitrogen flash-freezing considerably shortened the freezing time. The sensory score of chicken chops treated with liquid nitrogen flash-freezing at -100 ℃ (70.83 points) was the closest to that of chicken chops refrigerated at 4 ℃ (84.00 points), whereas treatment with liquid nitrogen flash-freezing at -120 ℃ caused cracks on the surface of the chops. The water holding capacity of pre-made chicken chops can be effectively maintained by liquid nitrogen flash-freezing. The centrifugal loss rate of chicken chops treated with liquid nitrogen flash-freezing at -100 ℃ was 20.95%, whereas that of chicken chops treated with slow freezing at -18 ℃ was as high as 32.84%. A significant difference was detected in pH among the different groups (P<0.05), but not in color (P>0.05). The texture indices of the liquid nitrogen flash-freezing groups were generally superior to those of the -18 ℃ slow-freezing group. Compared with slow freezing at -18 ℃, the malondialdehyde content of chicken chops treated with liquid nitrogen flash-freezing at -80 and -100 ℃ decreased by 22.54% and 15.02%, respectively, whereas their glutathione peroxidase activity increased by 66.13% and 132.58%, respectively. In conclusion, compared with the slow freezing at -18 ℃, chicken chops treated with liquid nitrogen flash-freezing retained superior eating quality. It is recommended that the processing temperature be maintained between -80 and -100 ℃.
    19  The Effect of 60Co-γ-irradiation Assisted Packaging Materials on the Storage Quality of Fresh Chicken Meat
    WEN Xiaomei LAN Bifeng WU Junshi LUO Pengyu LIANG Shumin
    2024, 40(1):158-172. DOI: 10.13982/j.mfst.1673-9078.2024.1.1584
    [Abstract](83) [HTML](103) [PDF 2.46 M](370)
    Abstract:
    To provide theoretical guidance for prolonging the storage period of fresh chicken meat, guaranteeing its quality safety and expanding the circulation area, fresh chicken meat was used as the research object in this study, the effects of different irradiation doses (0, 2, 4, 6 kGy) and packaging materials (PE, PA/PE, PVDC/PE) on the quality of fresh chicken meat during storage at 4 ℃ were studied, based on the measurements of the drip loss rate, TVB-N, TBA, color difference and total bacteria count. The results showed that irradiation could effectively kill the microorganisms in chicken meat and inhibit the growth of microorganisms. However, after irradiation, the TBA value increased significantly (P<0.05), and the lipid oxidation induced by irradiation had a great effect on the quality of chicken meat. The examinations on irradiation combined with different packaging materials showed that the quality of chicken meat in the PA/PE packaging group was better during storage, and after irradiation, the TBA value changed little, ranging from 0~0.76 mg/kg for individual sample groups, and their colors were quite good during storage, with the a* value increasing up to 5.12 (4 kGy). Taken together, PA/PE packaging combined with irradiation at a dose of 4 kGy was the optimal option to ensure the safety and quality of fresh chicken meat, and extend the storage period at 4 ℃ to 18 days.
    20  Comparative Analysis of Chewing Processing and Bolus Characteristics of Sweet Potato Subjected to Different Cooking Methods
    FAN Huiping MA Chenchen SUO Biao ZHANG Yanjie LI Zhen CHEN Yumeng AI Zhilu
    2024, 40(1):173-182. DOI: 10.13982/j.mfst.1673-9078.2024.1.0029
    [Abstract](76) [HTML](117) [PDF 4.77 M](320)
    Abstract:
    To explore the masticatory processing behaviors and bolus characteristics of sweet potato subjected to different cooking methods, this study investigated the effects of cooking methods on the texture, starch content and the short- range ordered structure of sweet potato. Mastication tests were conducted, and the moisture content, mastication parameters, saliva secretion, bolus characteristics and starch digestion of the bolus were analyzed through tracking the process from chewing to the swallowing point of the subjects. The results showed that the hardness, elasticity, adhesiveness, cohesion, chewiness and recoverability of the inverted steamed sweet potato fries and the dried boiled sweet potato slices were significantly higher than those of the sweet potato products cooked by other four methods (P<0.05). The total starch content and the content of short-range ordered starch structure in the cooked sweet potato products were significantly lower than those in fresh sweet potato (P<0.05). In the chewing tests of different cooked sweet potato products, there were significant differences in the chewing parameters, such as one bite size, number of chews, chewing time and eating rate (P<0.05). The mean median particle size (D50) (1.55~2.65 mm) of the recovered bolus was positively correlated with the sample’s hardness, adhesiveness and chewiness (P<0.05). The differences in sample’s morphology and subject’s subjectivity led to significant differences in the mean amount of added saliva (0.41~0.94 g/g dry basis) and mean saliva flow rate (45.92~72.65 mg/s) during chewing (P<0.05). The average total starch digestibility after chewing was the highest for the steamed sweet potatoes (88.35%) with the microwaved sweet potatoes having the lowest average total starch digestibility (34.32%). This study laid a theoretical foundation for further study on digestive characteristics and deep processing of sweet potato.
    21  Preparation of Maillard Reaction Products derived from Pea Protein Enzymatic Hydrolysate and Improvement of the Flavor and Storage Stability of Low-sodium Plant-based Meat
    XIE Yuxi SONG Xueying ZHAO Mouming WU Jun LUO Donghui LUO Yanhua LIANG Chunhong ZHANG Jianan SU Guowan
    2024, 40(1):183-193. DOI: 10.13982/j.mfst.1673-9078.2024.1.1548
    [Abstract](90) [HTML](104) [PDF 3.54 M](361)
    Abstract:
    The effect of Maillard reaction products derived from pea protein enzymatic hydrolysate (PPIHM) on the quality of low-sodium plant-based meat was explored. Pea protein was used as the raw material, and was pretreated by protease hydrolysis combined with transglutaminase cross-linking, followed by the addition of 3.0% glutathione and 1.0% reducing sugar (xylose: ribose = 1:3) to the pea protein enzymatic hydrolysate for the Maillard reaction to prepare PPIHM. PPIHM showed good roasted aroma, umami and antioxidant capacity. Different mass fractions (1%, 3%, and 5%) of PPIHM were then added to low-sodium plant-based meat. Systematic investigation revealed that PPIHM could significantly improve the perceived meat aroma and delay the oil and protein oxidation of plant-based meat, while also maintaining its rigidity and stability. The sensory evaluators showed the highest preference and acceptance for the 3% PPIHM plant-based meat group, while the 5% PPIHM plant-based meat group had the best antioxidant capacity and storage stability, with a peroxide value of only 0.0097 g/100 g on the 12th day of storage. These results demonstrate that adding PPIHM is an effective means to improve the flavor and storage performance of low-sodium plant-based meat. Our findings can serve as a theoretical basis and method guidance for further exploring the application of PPIHM as a natural reactive meat flavor in low-sodium plant-based meat.
    22  Comparison of Color and Phenolic Composition of ‘Marselan’ Dry Red Wines from Different Sub-regions of the Eastern Foothills of the Helan Mountains, Ningxia
    WANG Chi LAN Yibin DUAN Changqing HE Fei
    2024, 40(1):194-204. DOI: 10.13982/j.mfst.1673-9078.2024.1.0091
    [Abstract](97) [HTML](113) [PDF 5.05 M](465)
    Abstract:
    This study was conducted to explore the terroir-specific characteristics of the phenolic compounds and colors of ‘Marselan’ dry red wines produced in various sub-regions of the eastern foothills of Helan Mountain in Ningxia. CIELAB color parameters and phenolic fractions of ‘Marselan’ dry red wines from Helan (HL), Xixia (XX), Yongning (YN), and Qingtongxia (QTX) were quantitatively characterized. No significant differences in color parameters were observed among the wine samples from these sub-regions. Color was found to correlate more significantly with vintage. Total monomeric anthocyanins were significantly higher in XX (69.83 mg/L) than in YN (19.37 mg/L); myricetin was significantly higher in HL and QTX (6.98 mg/L) than in YN (2.37 mg/L); procyanidin B, (-)-epicatechin, and (-)-epigallocatechin were significantly higher in XX than in YN; HL had significantly higher gentisic acid (2.44 mg/L) than XX (1.50 mg/L) and YN (1.31 mg/L); XX had significantly higher 3-hydroxycinnamic acid (46.37 mg/L) than QTX (32.14 mg/L) and YN (26.44 mg/L); HL (41.63 mg/L) also contained higher 3-hydroxycinnamic acid content than YN. Case-by-case discriminant analysis based on the phenolic contents clearly distinguished wine samples from different sub-regions. Correlation analysis found significant negative correlations between malvidins, petunidins, flavonols, and flavanols in the wines and L* and b* values. Significant positive correlations with a* values were found. ‘Marselan’ dry red wines from the Eastern Foothills of the Helan Mountains in Ningxia were not found to differ significantly in color, but displayed significant terroir specificity in phenolic composition.
    23  Water Absorption and Processing Characteristics of Dried Jujube under Different Temperatures Following Long-term Storage
    YIN Xiaolin HU Xin LI Biansheng DING Zhenzhen RUAN Zheng LI Dandan JIN Tingfei
    2024, 40(1):205-213. DOI: 10.13982/j.mfst.1673-9078.2024.1.0117
    [Abstract](87) [HTML](102) [PDF 6.83 M](344)
    Abstract:
    Jujube (Ziziphus jujuba Mill.) is a raw material used as both a medicine and food. It has attracted widespread interest concerning its health functions, storage and processing characteristics. The long-term storage of dried jujube can lead to severe dehydration, which causes the pulp to harden, the peel to darken, and its commodity value to decrease. Two types of Xinjiang jujube, Junzao and Huizao, that were stored for approximately 1 or 13 months, were selected as research samples to investigate the patterns of soaking and water absorption/rehydration and its effect on the processing properties of dried jujubes. Moisture content of Junzao and Huizao after 13 months of storage was reduced by approximately 50% compared with that of Junzao and Huizao after 1 month of storage. The duration of storage also affected fruit shape, pulp texture and composition, but the dried jujubes subjected to long-term storage retained good processing and utilization value. The water absorption curve of dried jujube was mostly logarithmic, and water absorption was affected by water temperature and the variety of dried jujube. Low-field magnetic resonance imaging demonstrated that water mainly infiltrated into the jujube fruit through the pedicle and surface. When the moisture content of Junzao and Huizao stored for about 13 months was 43.18 g/100 g on a dry mass basis (db) (T=40 ℃ ) and 41.06 g/100 g db (T=60 ℃ ), respectively, the jujubes underwent a slight softening, which was ideal for pitting and slicing. When the dry jujube was soaked until the moisture content, on a dry mass basis, was 129 g/100 g, it was fully softened and moisture was evenly distributed, which was ideal for beating and produced jujube pulp with good particle size and color.
    24  The Influences of Three Quality Improvers on the Quality of Fermented Corn Noodles Based on Principal Component Analysis and Response Surface Methodology
    ZHANG Shan REN Chuanying CHENG Jianjun WANG Shuai
    2024, 40(1):214-223. DOI: 10.13982/j.mfst.1673-9078.2024.1.1626
    [Abstract](103) [HTML](121) [PDF 6.87 M](411)
    Abstract:
    In this study, the traditional fermented corn noodles were produced from the raw material, corn. The effects of quality improvers on the quality of fermented corn noodles were studied using fermented corn noodle quality evaluation model that was established by principal component analysis (PCA) of cooking qualities and texture properties of noodles. Response surface analysis was used to examine the influences of different quality improvers and their interactions on the quality of fermented corn noodles, and the proportion of quality improvers was optimized. The results showed that the first two principal components F1 and F2 contributed 58.31% and 25.41%, respectively, with the cumulative contribution being 83.72%. According to the PCA results, a model for evaluating the quality of fermented corn noodle was established. The effects on the quality of fermented corn noodles exerted by quality improvers decreased in the order of guar gum, salt and wheat gluten. The interactions between salt and guar gum, and between guar gum and wheat gluten had significant effects on the quality of fermented corn noodle (P<0.05). The optimal proportion of quality improvers was: salt 0.87%, guar gum 0.53% and wheat gluten 6.18%.
    25  Effects of Thawing Methods on the Physicochemical and Textural Properties of Frozen Fish Balls
    SHENG Qianru WANG Juan
    2024, 40(1):224-232. DOI: 10.13982/j.mfst.1673-9078.2024.1.1410
    [Abstract](111) [HTML](85) [PDF 5.59 M](427)
    Abstract:
    Six different thawing methods, air thawing, low-temperature thawing, hot water thawing, ruuning water thawing, ultrasound thawing and microwave thawing were used, in order to investigate the effects of different thawing methods on the quality of frozen fish ball. In this study, the thawing time, water holding capacity, color, pH, total volatile basic nitrogen (TVB-N) content, thiobarbituric acid-reactive substance (TBARS) content, texture characteristics and microstructure were measured to evaluate the effects of thawing on frozen fish balls. The results showed that the microwave thawing could make the balls thawed rapidly, but the water holding capacity and texture index of the balls after thawing were poor, with local overheating being obvious. Ultrasound thawing led to a better water holding capacity, and its thawing loss, TVB-N content and TBARS content were being lower (only 2.60%, 3.85 mg/100 g and 0.54 mg MDA/kg, respectively). Compared with other thawing methods, low-temperature thawing had the lowest thawing loss and cooking loss (2.24% and 1.97%, respectively), with the hardness, springiness, gumminess and chewiness value being better than others. The results of scanning electron microscopy showed that the muscle structure of the fish balls thawed at a low-temperature was compact, with minimal damage. Air thawing promoted a high degree of protein degradation, with the TVB-N content being the highest (5.60 mg/100 g). The running water thawing also enhanced protein degradation and its TVB-N content was insignificantly different from that for air thawing. There were no significant differences in the color and pH among the fish balls thawed by different methods. Taken together, ultrasound thawing could maintain the water holding capacity and had a relatively smaller effect on protein and lipid, thus it was an ideal thawing method for defrosting fish balls. However, the parameters of ultrasound thawing still need to be studied. The low-temperature thawing led to a texture property, thus, may be suitable for normal cooking by consumers.
    26  Changes in the Nutritional Content and Rheological Properties of Mango Pulp during Accelerated Ripening
    HU Longxiao LIU Lin TAN Baoyao ZHAO Lei WANG Kai
    2024, 40(1):233-239. DOI: 10.13982/j.mfst.1673-9078.2024.1.1481
    [Abstract](102) [HTML](83) [PDF 5.28 M](508)
    Abstract:
    Accelerated ripening is an important technique to promote fruit ripening, and the duration of accelerated ripening has an important impact on the nutritional content of mangos. This study investigated nutritional changes and rheological properties of mangos after different durations of accelerated ripening. After accelerated ripening for 108 h, the starch content of mangos decreased from 7.95% to 0.06%, pectin content decreased from 15.79 to 3.74 g/kg, total flavonoid content increased by 0.24 mg/g, while the total phenolic content showed no significant change. Peroxidase activity and malondialdehyde content increased with a longer duration of accelerated ripening. However, peroxidase activity plateaud after 72 h of accelerated ripening, while malondialdehyde content did not show further significant increase after 60 h, indicating that the extent of cell membrane damage did not change significantly after 60 h of accelerated ripening. The rheological results showed that mango pulp was a non-Newtonian fluid and weak gel system, and its viscosity decreased gradually with increasing duration of ripening. Based on the comprehensive assessment of nutritional content, peroxidase activity, malondialdehyde content and rheological results, the overall quality of mango pulp was relatively good after accelerated ripening for 72 h. These results can provide theoretical and practical guidance for the quality control of mangoes during accelerated ripening.
    27  Comparison of the Stability and Quality of Sucrose Ester Binary Emulsifiers on Compound Bone Broth Emulsion
    HE Ziqiong YANG Yang ZHANG Liyan
    2024, 40(1):240-248. DOI: 10.13982/j.mfst.1673-9078.2024.1.0101
    [Abstract](102) [HTML](105) [PDF 3.49 M](428)
    Abstract:
    To improve the emulsion stability and quality of compound bone broths, a sucrose ester was mixed with each of monoglycerides, soy lecithin, and microcrystalline cellulose to produce binary emulsifiers with different hydrophilic- lipophilic balance (HLB) values. The effects of various HLB values on the stability of the compound bone broth emulsions, the underlying causes, and the sensory quality of broth emulsions were studied. The results showed that when the HLB of sucrose ester+monoglyceride was 8, the zeta potential of the emulsion was -34.8 mV, viscosity was 8.91 Pa•s, and the average particle size was D[4,3] (1 496 nm). When the HLB value of sucrose ester+soybean lecithin was 12, the zeta potential value of the emulsion was (-38 mV), viscosity was 8.94 Pa•s, and average particle size was D[4,3] (725.1 nm). When the HLB value of sucrose ester+microcrystalline cellulose was 10~12, the zeta potential of the emulsion was (-26.3 mV)~(-29.3 mV). All compound bone broth emulsions showed good emulsion stability. The stabilizing effect of the sucrose ester+soy lecithin binary emulsifier on compound bone broth emulsion was superior to that of the remaining two binary emulsifiers, resulting from a significant reduction in the diameter of emulsion droplets (P<0.05), and increases in the surface charge density and emulsion viscosity. These changes ensured a more uniform dispersion of the droplets and they were less likely to agglomerate and float. A substantial synergistic effect was obtained when the sucrose ester was mixed with the monoglyceride or soybean lecithin, but not with microcrystalline cellulose. The mixing ratio of the binary emulsifiers considerably influenced the consistency, color, and acceptability of compound bone broth emulsions, whereas it effect on aroma and taste, which was related to emulsion stability, was relatively diminished.
    28  Preparation and Analyses of Textural Quality and Flavor Substances of Goji Berry Yoghurt
    LUO Yeming FU Hongfei SUOLANG Jiacuo ZHOU Yuan
    2024, 40(1):249-261. DOI: 10.13982/j.mfst.1673-9078.2024.1.0067
    [Abstract](132) [HTML](108) [PDF 7.68 M](369)
    Abstract:
    Goji berry puree and pure milk were chosen as the main raw materials, and the formula and fermentation conditions of goji berry yogurt were optimized by single factor tests and orthogonal experiments. Texture parameters of the yoghurts were determined by a physical property analyzer, while their composition and content of volatile flavor substances were analyzed by headspace-solid phase microextraction-gas chromatography-mass spectrometry. The results showed that the optimal formula of goji berry yogurt was as follows (on basis of the pure milk (mass fraction): goji berry puree, 5%; milk powder, 3%; sucrose, 10%; starter culture, 0.1%, fermentation temperature, 42 ℃; fermentation, 8 h. The sensory score for the optimized goji berry yogurt was 85.2, with the firmness as 28.32 g, consistency as 379.31 g•sec, cohesiveness as -18.35 g, viscosity index as -16.98 g•sec, and acidity as 100.20 °T. The correlation analysis showed that the firmness of the goji berry yoghurt was significantly and negatively correlated with the color and tissue status (r<0.001), and the consistency was positively correlated with the taste sensation (r<0.01), the color was significantly and positively correlated with the tissue status (r<0.001), the tissue status was significantly and positively correlated with the flavor (r<0.05), and the flavor was significantly and positively correlated with the taste sensation (r<0.001). The goji berry yogurt contained 32 of kinds of volatile flavor substances, with the content being 1 417.09 μg/kg. Compounds such as aldehydes in goji berry puree were the main flavor sources of the characteristic flavor of goji berry yogurt. The developed goji berry yoghurt is soft in color, and has a uniform texture, moderate consistency, delicate taste sensation, appropriate acidity and sweetness, with high nutrition and rich flavor.
    29  Improvement of Quality and Aromatic Components of High-salt Dilute Soy Sauce by Increasing the Proportion of Bran
    WU Qianyu GENG Yuhuan
    2024, 40(1):262-271. DOI: 10.13982/j.mfst.1673-9078.2024.1.1574
    [Abstract](98) [HTML](89) [PDF 5.87 M](362)
    Abstract:
    Soybeans, flour, and bran were used as raw materials to prepare high-salt dilute soy sauce, and the dose dependent effects of bran addition on the soy sauce physicochemical indices and antioxidant capacity were explored. Headspace solid phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) were performed to detect and analyze the aromatic components of soy sauce samples. Koji protease activity was highest, and amino acid nitrogen (0.87 g/mL) and total nitrogen (1.81 g/mL) were most abundant when the ratio of soybean, flour, and bran was 7:2:1 (S2). Compared with the control soy sauce, the antioxidant capacity of soy sauce fortified with bran was significantly enhanced; the respective reducing powers of S2 (7:2:1) and S3 (7:1:2) were 30.38% and 20.85% higher than that of S1 (7:3:0) at the end of fermentation. The respective DPPH free radical scavenging rates of S2 and S3 were 31.818% and 28.59% higher than that of S1 (P<0.05). Sixty volatile flavor compounds were detected in soy sauce with bran added. Bran addition significantly increased the types and contents of aldehydes, acids, and heterocyclic compounds in soy sauce, enriching the aroma components of soy sauce richer, and improving its quality and flavor. These results are informative, promote the high-value utilization of bran in soy sauce production, and significantly contribute to the optimization of soy sauce brewing technology.
    30  Establishment and Application of An Improved QuEChERS Combined with GC-MS/MS Method for the Detection of Six Non-standard Pesticides
    SU Yahang CHE Yingxin LI Yongli LIU Jun TANG Shi LI Haitao TANG Junni
    2024, 40(1):272-280. DOI: 10.13982/j.mfst.1673-9078.2024.1.1511
    [Abstract](109) [HTML](109) [PDF 4.02 M](802)
    Abstract:
    In this study, given the fact that six kinds of pesticides are mainly detected in fruits and vegetables imported and exported from the European Union and Japan, but there are no detection standards in China's national food safety standards, an improved QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) extraction and purification method combined with Gas Chromatography-Tandem Mass Spectrometry (GC-MS/MS) detection method was established. This method could rapidly detect six pesticide residues such as sodium o-phenylphenol and dimethyl dichloromethane in leafy vegetable substrates. The sample was extracted with acetonitrile, salted out with anhydrous MgSO4 and NaCl to remove water, purified with N-propylethylenediamine (PSA) solid-phase material, and analyzed and detected using GC-MS/MS in multiple reaction monitoring (MRM) mode. The results showed that the six pesticides had a good linear relationship in the range from 0.002 to 0.5 μg/mL, with all the correlation coefficient R being greater than 0.998, the detection limit (RSN=3) being 0.15-2.32 μg/kg, the average recovery being 84.50%~106.23%, and the relative standard deviation being 0.96%~8.35%. The results are in accordance with the requirements of the quality control procedures for pesticide residues in the European Union and the pesticide limit requirements of the "uniform standard" for agricultural residues in Japan and the European Union. The established detection method was further applied to the detection of high-incidence pesticide substances in other vegetables, with good detection results. The method established in this study is reproducible, time efficiency, and easy to operate, which provides a new detection technology and basis for the detection of various pesticide residues such as sodium o-phenylphenol and dimethyl dichloromethane.
    31  Determination of the New Illegal Additive Acetohydroxamic Acid in Wheat Flour, Wheat-flour Products and Wheat-flour Additives using UPLC-MS/MS
    YUAN Lijie ZHAO Guangsheng ZHANG Peiyi YUAN Yanglei CHEN Xinxin MAO Jing
    2024, 40(1):281-287. DOI: 10.13982/j.mfst.1673-9078.2024.1.0161
    [Abstract](123) [HTML](96) [PDF 3.30 M](282)
    Abstract:
    An analytical method based on ultra-performance liquid-chromatography-tandem mass spectrometry was developed for the determination of a new illegal additive, acetohydroxamic acid (AHA), in wheat flour, wheat-flour products, and wheat-flour additives. Five representative samples (wheat flour, a wheat-flour additive, dough, noodles, and steamed bun) were selected as the study matrices. AHA was first extracted using ultrasonic and vortex oscillation with methanol as the extraction solvent, subjected to high-speed centrifugation and filtration, and then separated using a T3 column (2.1 mm×150 mm, 3 μm) with a mixture of 0.10% (volume fraction) aqueous formic acid and methanol as the eluting mobile phase. Finally, it was quantified using an external standard method using an atmospheric pressure chemical ionization source (APCI) and multiple reaction monitoring mode. The results showed that the solvent and matrix standard curves of AHA had good linearity in the 0.075~5.00 mg/L range, with correlation coefficients>0.999. The limit of detection was 0.25 mg/kg, the spike recovery rate was 78.49%~108.61%, and the relative standard deviation (RSD, n=6) was 1.67%~7.50%. This method is suitable for the determination of AHA in wheat flour, wheat-flour products and wheat-flour additives, exhibiting good anti-interference capacity and high accuracy.
    32  Analysis of the Quality Difference of Xylose Lysine Deoxycholate Agar for Food Testing
    LIU Shuai ZHANG Jie CHEN Yiwen XU Miaomiao LI Xian WANG Hui CHEN Boxu CUI Shenghui ZHOU Wei
    2024, 40(1):288-295. DOI: 10.13982/j.mfst.1673-9078.2024.1.0004
    [Abstract](61) [HTML](96) [PDF 3.12 M](676)
    Abstract:
    In order to enrich the quality control strains during the acceptance of media for food microbiology testing in China and improve the quality of commercial culture media, the medium acceptance method specified in GB 4789.28-2013 was used in this experiment. Salmonella typhimurium ATCC 14028, Shigella fowlerii CMCC 51572, Staphylococcus aureus ATCC6538 and Escherichia coli ATCC 25922 were used as the references. Six strains of Salmonella typhimurium, one strain of Shigella fowleri, four strains of Staphylococcus aureus and five strains of Escherichia coli were selected for evaluating the physical indexes such as appearance, pH value and moisture content, as well as the microbial indicators such as growth rate and selectivity of four brands of XLD. The results showed that the colors of dry powders of four medium brands, A, B, C and D, were pink, light pink, earthy yellow and pinkish white. Their moisture contents were 5.55%, 5.92%, 5.46% and 5.94%, respectively. The dosages were 5.54, 5.89, 5.69, and 5.70 g in 100 mL respectively. All the pH values were 7.4. The growth rates of the six Salmonella typhimurium strains and the Shigella fowleri strain on the four brands of media were not lower than 0.7, whilst none of the four of Staphylococcus aureus strains grew, and all the five Escherichia coli strains had a growth index G lower than 5. In summary, the four brands of XLD all conformed to GB 4789.28-2013, the strain selected for this experiment can be used as a supplementary strain for the acceptance of XLD quality control strains.
    33  Comparison of Complex Chromogenic Systems for the Detection of Total Flavonoid Content in Corn Silk
    XU Xiaowei LI Zhugang LI Xiaojuan WANG Xun ZHAO Wei LENG Chunxu ZHAO Xi LU Jie HAN Hao
    2024, 40(1):296-303. DOI: 10.13982/j.mfst.1673-9078.2024.1.0106
    [Abstract](149) [HTML](120) [PDF 5.23 M](359)
    Abstract:
    UV-visible spectrophotometry was employed to establish the most suitable complex chromogenic system for detecting corn silk total flavonoids (CSTF). CSTF extraction was performed using 60% ethanol as the solvent, and fractional extraction was undertaken using different polar solvents, which gave the normal hexane (n-hexane), ethyl acetate and normal butanol (n-BuOH) extracts. Using different types of corn silk flavonoids as reference standards, comparisons were made with the total flavonoid content of the ethyl acetate and n-BuOH extracts under the NaNO2-Al(NO3)3/AlCl3-NaOH and KAc-Al(NO3)3/AlCl3 complex chromogenic systems. High-performance liquid chromatography (HPLC) was also performed on the flavonoid content of the ethyl acetate and n-BuOH extracts to identify the most suitable reference standard and chromogenic system.The flavonoid content of the ethyl acetate and n-BuOH extracts detected by HPLC was 83.45% and 57.36%, respectively. The comparative results indicated that the KAc-Al(NO3)3 complex chromogenic system with rutin as the reference standard was the closest to the CSTF detected by HPLC. In conclusion, the KAc-Al(NO3)3 complex chromogenic system was the best method for quantifying the total flavonoid content in corn silk and in each extract, and the flavonoid content of CSTF extract detected using this method was 1.08%. Therefore, this is a simple and accurate technique that can serve as a basis for the development, utilization and quality control of corn silk flavonoids.
    34  Research Advances in the Efficient Prevention and Control of Foodborne Listeria monocytogenes by Bacteriophages
    LIANG Xinwen LIU Zhenjie ZHANG Jumei CHEN Moutong WU Qingping
    2024, 40(1):304-311. DOI: 10.13982/j.mfst.1673-9078.2024.1.0318
    [Abstract](140) [HTML](120) [PDF 3.10 M](883)
    Abstract:
    Listeria monocytogenes is an important foodborne pathogen that poses a major threat to food safety owing to its resistance to high salinity and tolerance to a wide range of pHs and temperatures. Owing to the rapid emergence of antibiotic-resistant bacteria, efficient control of L. monocytogenes in food is an important challenge for the food industry and public health agencies. Bacteriophages have shown great potential in preventing and controlling foodborne pathogens owing to their strong host specificity, good safety profile, and fast reproduction rate. Several studies have shown bacteriophages to be natural, green bactericides with promising prospects for preventing and controlling pathogenic microorganisms in different types of food products. Although the application of bacteriophage-based biocontrol continues to face challenges, such as a narrow host range and the potential of resistance development, this approach will play a crucial role as a safe and effective method for preventing and controlling L. monocytogenes in food. In this article, recent research advances in the isolation and rapid detection of L. monocytogenes-specific phages in food as well as the use of bacteriophages for the efficient prevention and control of this bacterial pathogen are reviewed. This review paper provides a reference for the development of new and efficient bacteriophage-based prevention and control techniques.
    35  Research Advances in Quality Control and Intelligent Monitoring Technologies for Aquatic Prepared Products
    ZHAO Xi CHEN Jian LI Huan WANG Yanbo
    2024, 40(1):312-318. DOI: 10.13982/j.mfst.1673-9078.2024.1.1630
    [Abstract](93) [HTML](137) [PDF 4.06 M](371)
    Abstract:
    The increasing popularity of prepared foods in recent years can be attributed to the convenience they offer. However, oxidation, microbial activity, and endogenous enzyme degradation are vital factors that cause deterioration of the quality of aquatic prepared foods. Therefore, regulating quality deterioration and monitoring the quality change of aquatic prepared products are crucial steps for maintaining product quality and promoting the development of the prepared food industry. In this review, the methods used to control the quality of aquatic prepared products (i.e., from the preparation process to the packaging methods and sterilization technology) and intelligent quality monitoring methods used in the industry (i.e., from detection labels to machine learning and full-chain monitoring) are summarized and discussed. This review aims to serve as a reference and basis for the quality control and intelligent monitoring of aquatic prepared products and to provide support for the high-quality development of the prepared food industry.
    36  Research Progress on Microorganisms in Chi-flavored Baijiu
    SU Zejia XIONG Ruobing LIANG Jinglong FEI Yongtao BAI Weidong ZHAO Wenhong
    2024, 40(1):319-324. DOI: 10.13982/j.mfst.1673-9078.2024.1.1441
    [Abstract](155) [HTML](108) [PDF 3.22 M](379)
    Abstract:
    Chi-flavored Baijiu is a liquor with a derivative flavor of Chinese Rice baijiu. It has mellow sensation, unique fermented black bean flavor, mellow and sweet taste, with a clean aftertaste. It is one of the representative foods/beverages of the Lingnan region in Guangdong province, China. In recent years, the trend of Baijiu goes towards low level of alcohol and more comfortable feeling, which conforms to the development characteristics of Chi-flavor Baijiu. But, to open further the market and meet market requirements, product flavor composition is the key. The structure of fermentation microorganisms has a significant impact on flavor formation. Therefore, this paper reviews the microbial structure of Chi-flavor Baijiu, the microorganisms affecting the Baijiu’s flavor, and microbial enhanced fermentation to improve Baijiu quality, in order to provide a reference for the further development of Chi-flavor Baijiu.
    37  Research Progress of Marine Vibrio Diversity in Beibu Gulf of Guangxi
    QIN Xinyi ZHAO Huaxian YANG Shu HUANG Jiongqing LIAO Nengjian LI Xiaoli JIANG Gonglingxia LI Nan
    2024, 40(1):325-331. DOI: 10.13982/j.mfst.1673-9078.2024.1.1522
    [Abstract](80) [HTML](98) [PDF 3.96 M](325)
    Abstract:
    Marine Vibrio plays a key role in the biogeochemical cycle, and certain pathogenic marine Vibrios can threaten human health and cause economic loss in the aquaculture industry. In recent years, a lot of achievements have been made in studying the diversity of marine Vibrio in Guangxi Beibu Gulf, though there are still few reports that systematically summarize them. To better understand the species composition, communities distribution characteristics and driving mechanism of the marine Vibrio in the Beibu Gulf of Guangxi, this paper reviewed the research on the diversity of marine Vibrio in the Beibu Gulf of Guangxi based on the community structural characteristics, major environmental driving factors, and the community assembly mechanism. The results showed that the marine Vibrio diversity in Guangxi Beibu Gulf varies with sea area and season, with the main driving factors being nutrients such as total dissolved nitrogen, dissolved inorganic nitrogen, and total dissolved phosphorus. The community assembly process of the Vibrio community in Guangxi Beibu Gulf was dominated by random processes. In addition, this review provides an outlook for future studies on marine Vibrio diversity in Guangxi Beibu Gulf, aiming to provide new ideas for the prevention and control of pathogenic marine Vibrio outbreaks, and provide a theoretical reference for the research on the ecological function diversity of marine Vibrios and the high-value utilization of biological resources.
    38  Research Progress on the Application of Plant Essential Oil Microcapsules in Food Preservation
    LI Jiamin SUN Feilong SHI Yu LUO Rumeng JIA Guangfeng
    2024, 40(1):332-339. DOI: 10.13982/j.mfst.1673-9078.2024.1.1531
    [Abstract](191) [HTML](114) [PDF 6.25 M](635)
    Abstract:
    Food is easy to be contaminated by pathogenic bacteria during transportation and storage, which will affect food quality and threaten human health. Therefore, at present, the development of safe and effective natural preservatives is one of the important research directions for food preservation. The inherent antibacterial activity and antioxidant activity of plant essential oils make them have a broad application prospect in food preservation, and microencapsulation technology can improve the flavor of essential oils, maintain their chemical stability, reduce oxidative volatilization, while offering a slow- release effect, which can allow more effective food preservation. This paper reviews the preparation materials, preparation methods, preservation mechanism, and release mechanism of plant essential oil microcapsules and their applications in the preservation of fruits, vegetables, meat products, and other food products, to provide a reference for further research and application of plant essential oil microcapsules in food preservation.

    Editor in chief:李琳

    Inauguration:现代食品科技

    International standard number:ISSN 1673-9078

    Unified domestic issue:CN 44-1620/TS

    Domestic postal code:46-349

    • Most Read
    • Most Cited
    • Most Downloaded
    Press search
    Search term
    From To