Volume 39,Issue 2,2023 Table of Contents

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  • 1  Characterization and Evaluation of the “Garlic Clove” Meat Structure in Processed Fish Products
    LI Xiang CAI Libin XIONG Shanbai HU Yang
    2023, 39(2):1-8. DOI: 10.13982/j.mfst.1673-9078.2023.2.0537
    [Abstract](276) [HTML](212) [PDF 25.29 M](1662)
    Abstract:
    Twelve grass carp fish fillets, with different degrees of processing, were prepared by controlling the amount of salt added, processing temperature, and processing time. Through comprehensive use of sensory evaluation, texture analysis, and tissue structure characterization, the characteristics of the “garlic clove” meat structure in processed fish fillets were described and criteria were established for their structural evaluation. Significant differences were observed among the sensory scores, textural properties, and tissue morphologies of the 12 groups of processed fish fillets. Processed grass carp fillets with a typical” garlic clove” meat structure can be prepared by controlling the amount of added salt, processing temperature, and processing time. The sensory score of the “garlic clove” meat structure in processed fish fillets was positively correlated with the textural parameters like hardness and adhesiveness (P<0.05), and negatively correlated with muscle fiber area (P<0.01). The texture and tissue structure parameters of processed fish fillets with excellent “garlic clove” meat structure were as follows: hardness 2 600~3 300 g, elasticity 0.56~0.74, chewiness 600~1 300 g, resilience 0.24~0.32, adhesiveness 240~350 g•sec, muscle fiber area 24 000~32 000 μm2, and intermuscular distance 16~21 μm. This data provides a technical reference for the reasonable evaluation and controllable preparation of fish fillets with “garlic clove” meat structure.
    2  Quality Change and Aroma Formation during Drying in Pre-made Cold Air-dried Wuchang Fish
    CHEN Fangxue DENG Yi SHEN Lingwei LI Dongsheng QIAO Yu WU Wenjin XIONG Guangquan WANG Lan LI Xin SHI Liu DING Anzi
    2023, 39(2):9-17. DOI: 10.13982/j.mfst.1673-9078.2023.2.0811
    [Abstract](164) [HTML](192) [PDF 25.43 M](1452)
    Abstract:
    Cured fish products are a traditional Chinese food, and these products are processed to extend storage duration, reduce losses, and improve flavor and nutritional value. Here, cold air-dried, salted Wuchang fish was compared with the undried salted fish to examine the effect of drying on product quality and to study the formation of aroma during the drying process. Specifically, the moisture content, total lipid content, total oxidation value, chromaticity, and fatty acids were quantified, in addition to electronic nose tests and gas chromatography–mass spectrometry (GC-MS) combined with the analyses of flavor and other indicators. The total oxidation value increased, whereas the moisture and total lipid contents decreased gradually with the prolongation of drying duration. The color of the fish dried for 48 h was yellowish near the abdomen and slightly dark on the back. During the drying process of Wuchang fish, over 50 types of volatile compounds in nine categories were detected using GC-MS, and the concentration of total volatile compounds increased from 277.04 to 1 219.22 ng/g. Eight types of odor-active compounds with the odor activity value (OAV) exceeding 1 were screened out. With the prolongation of drying duration, the concentration of volatile substances increased but the content of unsaturated fatty acids decreased gradually. With the prolongation of drying duration, the aroma intensified gradually, peaking after 48 h of drying.
    3  Preparation of Chicken Sausages Using Soybean-chicken Hydrolysate as A Substitute and Their Quality Changes
    ZENG Muzhi ZHAO Mouming WU Jun LUO Yanhua HUO Guochang YE Lihong ZHONG Fangfang SU Guowan ZHANG Jianan
    2023, 39(2):18-28. DOI: 10.13982/j.mfst.1673-9078.2023.2.1354
    [Abstract](211) [HTML](178) [PDF 18.46 M](1040)
    Abstract:
    To explore the effects of soybean-chicken hydrolysate (SCH) as a partial substitute for chicken breast and sodium chloride (NaCl) on the quality characteristics of chicken ham sausages and the feasibility of this substitution, ham sausages were prepared using different SCHs as the research objects. The contents of major components, thermal stability, texture, sensory quality, and storage stability were systematically analyzed, and combined with orthogonal experiments, the best compound formula of chicken ham sausage was optimized. Partial substitution of chicken breast meat and NaCl with SCH significantly improved the flavor, chewing characteristics, and storage quality of chicken sausages. The intensity of umami taste and chicken aroma was enhanced by approximately 15% and 20%, respectively, and the chewiness was increased by 71.86%. The optimal substitution formula was as follows: 20% chicken breast and 17% NaCl; the total substitution amount (dry) accounted for 2.0% of the total weight of the chicken sausage. The chicken sausages prepared with the optimal formula were nutritious and healthy (15.82% protein content and 1.59% sodium salt), had a favorable flavor (strong umami and rich chicken flavor, with a sensory score of 91.04), showed moderate texture (firmness of 6 416.63 N and chewiness of 3 974.41 N), and exhibited strong storage stability (TBARS value 0.37 mg MDA/kg, total bacterial colony 1.78 lg CFU/g), reaching the level of high-quality chicken sausages. Therefore, the raw and auxiliary materials of chicken sausages can be partially substituted with SCHs, with a positive effect on the quality improvement of the product.
    4  Process Optimization of Tenderizing Goose Meat with Papain
    ZHANG Huipeng LIN Yiyun YANG Huaigu TANG Daobang RUAN Qijun LIU Xueming ZOU Jinhao CHENG Jingrong WANG Zhitong LIN Yaosheng LIN Yaosheng
    2023, 39(2):29-35. DOI: 10.13982/j.mfst.1673-9078.2023.2.1045
    [Abstract](183) [HTML](369) [PDF 19.58 M](1763)
    Abstract:
    To improve the quality of goose meat, enzymatic tenderization with papain was used, and the effects of different treatment conditions on goose meat were analyzed. Based on the results of single-factor experiments, response surface methodology with four factors and three levels was applied for process optimization. Papain concentration (A), temperature (B), pH (C), and incubation time (D) were used as independent variables, and sensory characteristics were set as the response surface indicator. Through regression analysis, the optimal process conditions for obtaining the best sensory properties were enzyme concentration of 0.076%, temperature of 60 ℃, pH of 7.6, and incubation time of 55 min. The predicted score of sensory evaluation under these conditions was 82.5, which was significantly improved from the value without treatment (59.7). The shear force was 26.47 N, which was 38.83% lower than that before tenderization, and the overall quality was significantly improved. The actual sensory quality results were close to the model values, indicating that the model exhibited satisfactory predictive performance. This work provides preliminary data and builds a theoretical foundation to establish practical processing techniques for the enzymatic tenderization of goose meat.
    5  Development and Quality Analysis of Ready-to-eat Crayfish Snack
    XU Wensi YANG Qifu QIN Jiawei TAN Hui SHAO Liye YANG Pinhong HAN Qing
    2023, 39(2):36-44. DOI: 10.13982/j.mfst.1673-9078.2023.2.1147
    [Abstract](251) [HTML](187) [PDF 8.57 M](1321)
    Abstract:
    To fill the gap in instant crayfish snacks, a ready-to-eat crayfish snack was developed from crayfish tail as the raw ingredient. Single-factor and orthogonal experiments were performed with sensory scores as indicators to optimize the effects of salt, pepper, chili, chicken essence, monosodium glutamate, and cooking time on the crayfish snack, and the texture, nutritional value, and flavor of the products were evaluated. The optimal process and recipe was 2.5% salt, 13.3% pepper, 2.98% chili, 0.66% chicken essence, 0.66% monosodium glutamate, and 4 min cooking time, resulting in a sensory score of 76.66. The firmness of the crayfish tail was 119.83 g, elasticity was 3.88, cohesiveness was 0.92, and chewiness was 424.99. The developed crayfish product contained 55.85% moisture, 4.07% ash, 14.91% fat, 18.79% crude protein, and 3.87% total sugars. The total amino acid content was 155.93 mg/g, of which essential amino acids accounted for approximately 20%, and their scores were all greater than 1. Unsaturated fatty acids accounted for approximately 83% of total fatty acids. The free amino acid content was 2.53 mg/g, of which sweet and umami amino acids accounted for approximately 80%. The total nucleotide content was 71.28 mg/100 g, of which the content of inosinic acid was the highest at 58.13 mg/100 g, with the umami intensity of 0.29 g MSG/100 g. Therefore, the formulated ready-to-eat crayfish snack is rich in nutrients and flavor components and has favorable elasticity, firmness, and chewiness.
    6  Effects of Deodorization Methods on the Flavor Characteristics of Silver Carp Fish Scale Jelly
    HUANG Zenan SUN Ruowen XIONG Shanbai YIN Tao YOU Juan HU Yang
    2023, 39(2):45-54. DOI: 10.13982/j.mfst.1673-9078.2023.2.0668
    [Abstract](154) [HTML](174) [PDF 31.16 M](1413)
    Abstract:
    To improve the flavor and quality of fish scale jelly, silver carp fish scales were subjected to deodorization (ozone, vinegar, starch, ultrasound, and yeast) and used to prepare fish scale jelly. The effects of different deodorization methods on the flavor characteristics of the fish scale jelly were studied through sensory evaluation, electronic nose test, and headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS). Following treatment with ultrasound, yeast, vinegar, ozone, and starch, the sensory values of the fishy odor of fish scale jelly were 2.60, 1.80, 3.00, 4.10, and 1.40 respectively. Discriminant factor analysis on electronic nose test results of fish scale jelly revealed significant differences among samples subjected to different deodorization treatments. Among these, the ultrasonic group, yeast treatment group, and clear water group were the farthest apart, indicating a better deodorization effect. Through qualitative and quantitative analysis with GC-MS, 132 flavor compounds were detected in each treatment group, of which respectively 28, 24, 32, 51, and 36 volatile compounds were identified in the starch, ultrasonic, yeast, vinegar, and ozone treatment groups. All these numbers were lower than the number of volatile compounds in the clear water control group (64). Among the detected volatile substances, those with a larger contribution to the fishy odor included (E,E)-2,4- heptadienal, hexanal, (E)-2-hexenal, and octanal. Yeast treatment removed all amine substances, and the removal of several other fishy substances was the most obvious. In comprehensive comparison, yeast treatment was the most suitable method to remove the fishy odor from fish scales.
    7  Evaluation of the Effect of Acidic Electrolyzed Water in the Cleaning Process of Raw Ingredients in Pre-prepared Fish Dishes
    XU Hao JIAO Wenjuan ZHAO Tiantian ZHANG Yehui XIAO Xinglong ZHANG Xiaoyuan ZHANG Yousheng
    2023, 39(2):55-62. DOI: 10.13982/j.mfst.1673-9078.2023.2.1154
    [Abstract](153) [HTML](185) [PDF 11.27 M](1117)
    Abstract:
    Different types of electrolyzed water were used instead of ordinary water to wash raw tilapia, and the effects of acidic electrolyzed water on microorganisms on the fish surface, substances producing fishy odor, color difference, texture, and degree of lipid and protein oxidation during storage were examined. After washing the raw ingredients with acidic, weakly acidic, and neutral electrolyzed water instead of ordinary water for 3~10 min, microorganisms of the fish surface decreased from 34.55×103~24.21×103 CFU/g to 1.78×103~ 0.86×103 CFU/g, 9.21×103~2.21×103 CFU/g, and 12.52×103~5.12×103 CFU/g, respectively. The concentration of geosmin (GSM) - a substance producing fishy odor - decreased from 714.15 µg/L to 412.78~198.86, 519.21~312.21, and 612.12~385.12 µg/L, respectively. The concentration of 2-MIB, another substance producing fishy odor, decreased from 334.55~224.21 ng/L to 251.78~101.21, 279.21~128.21, and 312.52~205.12 ng/L, respectively. Under storage at 0~4 ℃, there were no significant changes in the total color difference (ΔE) value, firmness, elasticity, and chewiness of all samples (P˃0.05). However, after 8 days of storage, the total volatile base-nitrogen (TVB-N) content decreased from 40.11 mg N/100 g to 28.77, 32.54, and 32.22 mg N/100 g, respectively, in samples treated with the three types of electrolytic water. The content of thiobarbituric acid reactive substances (TBARS) decreased from 0.43 mg/100 g to 0.35, 0.41, and 0.38 mg/100 g, respectively, while the protein carbonyl value decreased from 1.63 nmol/mg to 1.35, 1.41, and 1.48 nmol/mg, respectively. Conversely, the thiol value increased from 6.83 nmol/mg to 7.35, 7.11, and 7.05 nmol/mg, respectively. Therefore, washing with electrolyzed water can effectively inhibit the degree of lipid and protein oxidation in fish. These findings provide a theoretical basis for the use of electrolyzed water to extend the storage period of pre-prepared fish dishes.
    8  Optimization of the Vacuum Tumbling Process for Quality Improvement of Beef Processed with Complex Low-sodium Salt Substitutes
    YANG Shujie CHEN Jinjin ZHANG Longtao XU Zhiyong ZHENG Baodong MIAO Song DENG Kaibo
    2023, 39(2):63-71. DOI: 10.13982/j.mfst.1673-9078.2023.2.1144
    [Abstract](183) [HTML](196) [PDF 43.56 M](1208)
    Abstract:
    To meet the industrial production requirements of beef processed with complex low-sodium salt substitutes, based on the results of single-factor experiments, the effects of curing liquid/meat ratio, tumbling time, and vacuum degree on the absorption rate of curing and rate of cooking loss were investigated. Subsequently, the vacuum tumbling process was optimized using the Box-Behnken design and response surface methodology, and the textural characteristics, shear force, moisture distribution, and microstructure were compared between beef cured statically and by tumbling. The optimal vacuum tumbling process parameters were the curing liquid/meat ratio of 32%, tumbling time of 3.0 h, and vacuum degree of 0.06 MPa. Under these conditions, the absorption rate of curing was 24.65%, while the rate of cooking loss was 26.39%. The firmness, elasticity, chewiness, and shear force of beef cured by tumbling were significantly lower than those of beef cured statically (P<0.05), and the relaxation time of bound and free water was also significantly lower in the former group (P<0.05). However, the peak area of free water relaxation was significantly higher in the tumbling group than in the static curing group (P<0.05). Scanning electron micrographs showed that the myofibril structures in the tumbling group were looser, with more fragmented structures attached onto the surface. Therefore, vacuum tumbling treatment can improve the textural characteristics, tenderness, and water retention of processed beef and induce microscopic fragmentation of myofibril structures. These findings provide technical support for the tumbling treatment of beef processed with complex low-sodium salt substitutes and build a theoretical basis for industrial production of nutritional low-sodium processed meat products.
    9  Process Optimization for Preserving the Color and Crispness of Mustard in Prefabricated Food
    YE Yang XIAO Chuanquan YUAN Xi ZHOU Yunong LUO Suqin YANG Qiang WANG Yang
    2023, 39(2):72-80. DOI: 10.13982/j.mfst.1673-9078.2023.2.1268
    [Abstract](254) [HTML](172) [PDF 28.12 M](1497)
    Abstract:
    To prevent the browning and softening of mustard-containing prefabricated food during processing and storage, the effects of different blanching conditions and different types and doses of color and crispness fixatives on the quality of mustard were analyzed using a single-factor orthogonal experimental design. Polyphenol oxidase (PPO) activity, peroxidase (POD) activity, chlorophyll content, shear force, color difference, and vitamin C content were used as indicators. The results indicated that the optimum process conditions were as follows: blanching at 90 ℃ for two min; using 0.06% (m/m) ascorbic acid, 0.12% (m/m) citric acid, and 0.012% (m/m) phytic acid for color preservation treatment for 40 min; and using 0.24% (m/m) calcium chloride for crispness preservation treatment for 30 min. Under these conditions, the inactivation rates for PPO and POD reached 90.40% and 66.03%, respectively, with the highest chlorophyll content being 93.60 mg/100 g. Compared with the control group, the vitamin C content, chlorophyll content, absolute value of a*, and shear force of the mustard treated with this process after 18 days of storage increased by 333.65%, 783.69%, 158.23%, and 318.84%, respectively. The processed mustard had a natural color, a crisp and tender taste, and the highest score in sensory testing. This process can effectively preserve the green color and crisp taste of mustard, thus providing a technical reference for processing other green leafy vegetables in prefabricated foods.
    10  Development Status of the Pre-prepared Aquatic Food Industry
    HUANG Yanyan LIANG Yantong LU Yunhui WU Jiamin LIANG Wanshi WANG Langhong ZENG Xinan
    2023, 39(2):81-87. DOI: 10.13982/j.mfst.1673-9078.2023.2.1040
    [Abstract](255) [HTML](254) [PDF 6.76 M](1426)
    Abstract:
    As a common food, aquatic products are favored by consumers due to their wide variety, low fat content, and high protein content. In recent years, the total amount of aquatic products cultivated and consumed in China has increased, and the pre-prepared aquatic food industry has emerged to meet the rising demand. Against the background of residents’ accelerating pace of life and the transformation and upgradation of the consumption structure, pre-prepared aquatic products have become a new trend in the food industry, with specific advantages, such as convenience, speed, health, nutrition, and safety. Since the pre-prepared aquatic food industry is involved in a wide range of content, many problems exist in the production and development of aquatic products. This article summarizes the development status of the pre-prepared aquatic food industry to better promote its innovation and progress. In particular, seven major problems and five key development trends in the pre-prepared aquatic food industry are proposed, and the development status of this industry is analyzed in depth to guide its healthy development.
    11  Application Prospects of Plant Resources in Improving the Quality of Pre-prepared Meat Dishes
    CHEN Peiyu LIU Xueming TANG Daobang WANG Xuping YANG Huaigu LIN Yaosheng CHENG Jingrong ZHU Mingjun RUAN Dong
    2023, 39(2):88-98. DOI: 10.13982/j.mfst.1673-9078.2023.2.0631
    [Abstract](168) [HTML](350) [PDF 704.63 K](698)
    Abstract:
    Meat products are an important source of protein for humans. Pre-prepared meat dishes are prone to peroxidation and microbial contamination during processing and storage, resulting in the loss of nutrients, color, and flavor. Therefore, the potential safety hazards of antioxidants in conventional meat products to human health have attracted people’s attention, and the application of natural plant resources to regulate the nutritional value and quality of meat products has been emphasized. Plants are rich in polyphenols, polysaccharides, organic acids, terpenoids, and other active ingredients, which produce potent regulatory effects on the color, oxidation, and flavor of meat products as well as the generation of harmful substances in these products. This article reviews the key quality changes in pre-prepared meat dishes, typical active substances of plants, and application status of natural active substances in pre-prepared meat dishes as well as explores the existing problems and future development direction of the current plant resources in improving the quality of pre-prepared meat dishes. This review can serve as a reference for high-quality development of the pre-prepared meat dish industry.
    12  Development Status and Trends of the Pre-prepared Food Industry in China
    WANG Juan GAO Qunyu LOU Wenyong
    2023, 39(2):99-103. DOI: 10.13982/j.mfst.1673-9078.2023.2.0388
    [Abstract](726) [HTML](819) [PDF 813.03 K](1545)
    Abstract:
    Pre-prepared foods are finished or semi-finished products derived from edible raw materials with a certain shelf life, and its industry has witnessed rapid development in recent years. Here, the definition, classification, development status, technical difficulties, market size, and major enterprises of pre-prepared foods are summarized, and their development trends are explored. The current major industrial provinces include Guangdong, Shandong, Fujian, Jiangsu, and Henan. The technical difficulties faced by the pre-prepared food industry primarily include food safety, sensory quality, nutritional quality, health functions, raw material quality control and traceability systems, quick freezing and thawing technology, packaging materials and technology, and intelligent equipment. Standardization, market segmentation, and cultivation and delivery of high-quality professionals and technical talent are expected to be the development direction of the pre-prepared food industry. This review can serve as a reference to guide scientific research and industry development in the field of pre-prepared foods.
    13  Analysis of Issues in the Development of the Pre-prepared Dishes Sector
    ZHAO Chaofan CHEN Shujun LI Wenbing YANG Guanghui NIU Wei HAO Zhiyong ZHANG Lizhen
    2023, 39(2):104-109. DOI: 10.13982/j.mfst.1673-9078.2023.2.1476
    [Abstract](440) [HTML](822) [PDF 5.44 M](1436)
    Abstract:
    In recent years, owing to the rise in popularity of new types of consumption behavior, including “lazy economy” and “cooking rookie,” pre-prepared dishes are becoming popular with consumers in China and worldwide. This is because of their merits, such as convenience and speed. Consequently, their market scale is gradually growing, with sound prospects for growth. Nevertheless, the sector is currently in the preliminary exploration phase in China, with standards and regulations, as well as the quality control system remaining inadequate. Fewer studies and reports consider the quality of the industry’s products and the status quo of the sector’s development. This study reviews the origin, concept, and development status quo of the pre-prepared dishes sector, and analyzes the current status and progress of research on testing methods, revision of standards, quality control, and risk assessment based on the status quo of quality safety of pre-prepared dishes in China. Moreover, this study offers a detailed analysis of the existing issues in the development of the pre-prepared dishes sector. Additionally, it proposes relevant response recommendations from the perspectives of product research and development, inter-disciplinary talent training, challenges in the development of enterprises, and management and control system. This aims to help readers gain a profound understanding of the pre-prepared dishes sector and provide information of reference value for the research and development of related sectors.
    14  Research Progress on Safety and Control of Pre-prepared Meat Dishes
    JIN Kenan ZHU Guangchao CHENG Dai YIN Shutao
    2023, 39(2):110-118. DOI: 10.13982/j.mfst.1673-9078.2023.2.1635
    [Abstract](479) [HTML](362) [PDF 692.79 K](1039)
    Abstract:
    With the rise of China’s “stay-at-home economy” and the acceleration of the pace of life, people’s consumption concepts and methods are undergoing profound changes. Traditional dishes, simple convenience foods, processed vegetables, and side dishes can no longer meet contemporary people’s pursuit of a high-quality life. As a result, pre-prepared dishes have emerged and further enriched the system of pre-prepared foods. Nowadays, prefabricated dishes have become one of the fastest growing categories in the food industry at home and abroad, and the processing technology and market are becoming increasingly mature. However, China’s products of pre-prepared dishes remain at their development stage, facing many issues, such as backward technology, mismatched standards, and prominent safety hazards. Problems such as agricultural and veterinary drug residues, illegal additions, and unhygienic conditions of the production origin are common safety hazards in the food industry. This study focuses on prominent safety hazards of pre-prepared dishes and provides a thorough understanding of the safety status of pre-prepared dishes in China (especially pre-prepared meat dishes). It analyzes the reasons for the formation of risks, discusses the identification and detection methods of risk factors, and proposes practical control measures for potential food safety problems, thereby providing a reference for the production and development of the prefabricated dish industry.
    15  The Immune-enhancing Effect of Drug-food Homologous Prescription Combined with Probiotics on Immunocompromised Mice
    LIU Haichun HU Chenxu WANG Jiaming LIU Rui LI Jinghua
    2023, 39(2):119-124. DOI: 10.13982/j.mfst.1673-9078.2023.2.0304
    [Abstract](194) [HTML](171) [PDF 868.29 K](669)
    Abstract:
    The aim of this study was to investigate the regulatory effect of the combination of drug-food homologous prescription and probiotics on the immune function of immunocompromised mice induced by cyclophosphamide (CTX). Except for the control group gavaged with normal saline, the mice in the other groups were intraperitoneally injected with CTX (100 mg/kg bw, for 4 days). Mice with similar body weights were randomly divided into 6 groups: control group, immunosuppression (model) group, drug-food homologous group III, drug-food homologous improvement group (IV), probiotics group (V), and IV-V combination group (VI). After the administration for 14 days, the effects of III, IV, V and VI on the thymus and spleen indices, phagocytic ability, spleen lymphocyte proliferation, NK cell activity and the levels of the formed IgG, IgA and IgM in immunodepressed mice were assessed. The results showed that the thymus index (1.46, 1.67, 1.50 and 1.92 g/kg) and spleen index (2.32, 2.54, 2.52 and 2.82 g/kg), phagocytic ability (1.39, 1.45, 1.42 and 1.43), spleen lymphocyte proliferation (1.39, 1.45, 1.42 and 1.43) and NK cells activity (0.35, 0.43, 0.36 and 0.71) were enhanced significantly (P<0.05) in the group III, IV, V and VI. IV, V and VI all significantly increased the levels of IgG (224.21, 163.21 and 253.35 mg/L), Ig A (34.86, 22.09 and 41.17 mg/L), and IgM (84.01, 78.23 and 100.28 mg/L) (P<0.05). These results suggested that the combination of the modified drug-food homologous prescription and probiotics could enhanced significantly the immune function of the CTX-immunosuppressed mice.
    16  Isolation and Purification of Lycium Barbarum Polysaccharides and Analysis of its in Vitro Anti-proliferative and Anti-inflammatory Activities
    QIAO Xifeng MA Congwei ZHANG Ying LIN Zhanle HUANG Jingxia LIN Li ZHANG Yongmin YANG Yiting DU Zhiyun
    2023, 39(2):125-134. DOI: 10.13982/j.mfst.1673-9078.2023.2.0297
    [Abstract](200) [HTML](203) [PDF 64.91 M](1189)
    Abstract:
    To investigate the effects of Lycium barbarum polysaccharides on the proliferation of Estradiol (E2) and progesterone (P) induced-mouse mammary gland epithelial cells (HC11) and LPS-induced inflammatory response in macrophages. Different components were obtained by water extraction, ethanol precipitation and column separation, and their molecular weights, and in vitro anti-proliferative and anti-inflammatory effects were preliminarily analyzed. The result showed that seven Lycium barbarum polysaccharides components were eluted out by DEAE-52-ion chromatography column and Sephadex G-50 gel column (GQ-01, GQ-02, GQ-03, GQ-04, GQ-05, GQ-06 and GQ-07, respectively), and their mean relative molecular weights were 113 ku, <1 ku, 367 ku, 613 ku, 81 ku, 110 ku, and <1 ku. Different Lycium barbarum polysaccharide concentrations (5, 25, 125, 250, 500, 1 000 μg/mL) were used to treat HC11 cells, and GQ-01, GQ-04 or Gq-06 at 250 μg/mL could significantly inhibit the proliferation of HC11 cells co-treated with E2 (25 μg/mL) and P (250 μg/mL) by 28.13%, 26.74% and 30.83%, respectively (P<0.01). GQ-01 and Gq-06 could regulate the G0/G1 and G2/M phase transition to affect the cell cycle distribution. Compared with the model group, Gq-06 could significantly inhibit the E2+P induced ERα level (decreased by 5.54%) (P<0.01). Compared with the model group, the production of NO in macrophages could be significantly inhibited by GQ-04 and GQ-06 (by 13.43% and 12.65%, respectively) (P<0.05). This research showed that GQ-06 may inhibit the excessive proliferation of mammary gland epithelial cells by affecting the cell cycle distribution and the level of ERα, and also suppress LPS-induced NO release to reduce the inflammatory response in RAW264.7 cells. This study will provide an important theoretical basis for further development of functional foods.
    17  Protective Effects of Hericium hericium Fermented with Probiotic Strains on Alcoholic Gastric Ulcer
    XUE Hongyang ZHANG Zhi BAO Ruimin JIANG Shilong XIE Qinggang GONG Yanni
    2023, 39(2):135-144. DOI: 10.13982/j.mfst.1673-9078.2023.2.0319
    [Abstract](160) [HTML](168) [PDF 94.87 M](1322)
    Abstract:
    In this experiment, three strains of Lactobacillus bulgaricus, Streptococcus thermophilus and Acetic acid bacteria were used to fermentat Hericium erinaceus. The DPPH free radical scavenging rates of the fermentation broth samples were compared to determine the optimal conditions for fermenting Hericium hericium. Mice with alcoholic gastric ulcer were subjected to intragastric administration with hericium ordosica probiotic fermentation broth. The contents of superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT) in gastric tissues and contents of interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor -α (TNF-α) in the serum of the mice with alcoholic gastric ulcer were measured. The results showed that the optimal fermentation conditions were as follows: initial pH 6.5; bacteria compound ratio 1:1:1 (Lactobacillus delbrueckii subsp. bulgaricus: Streptococcus thermophilus: Acetobacter); inoculation amount 8.8%; fermentation temperature 37.5 ℃; fermentation time 27 h. The colony number of the fermentation broth was 8.72×109 CFU/mL and the DPPH free radical scavenging rate of fermentation broth was 79.59%. Compared with the model group, the SOD and CAT levels increased by 71.38% and 99.89% whilst the MDA content decreased by 47.45% in gastric tissues of mice in high-dose group. The contents of TNF-α and IL-6 in the serum of mice decreased by 32.66% and 34.95%, respectively, and the content of IL-10 increased by 100.46%. The results showed that the probiotics fermentation broth of hericium had a protective effect on acute alcoholic gastric ulcer in mice. This study provides a theoretical support for the application of Hericium hericium, and a theoretical basis for the development of new products of Hericium hericium and probiotics.
    18  Concentration of Higher Alcohols in Xiaoqu Liquor Induced by Enhanced Fermentation with Strains of Bacillus
    HUANG Zhiguo LUO Runsen LI Yanzhong DENG Jie REN Zhiqiang WEI Chunhui
    2023, 39(2):145-151. DOI: 10.13982/j.mfst.1673-9078.2023.2.0251
    [Abstract](200) [HTML](175) [PDF 23.25 M](1576)
    Abstract:
    To explore the effect of Bacillus addition on higher alcohol content in Xiaoqu liquor, 13 Bacillus strains from Daqu were used as the starting strains. Through solid-state and liquid fermentation, two functional strains with the ability to reduce the higher alcohol content of Xiaoqu liquor were obtained. The strain B8 was identified as Bacillus licheniformis and strain B13 as Bacillus elezensis by morphological, physiological and biochemical analyses and 16S rDNA sequencing. The two functional Bacillus strains were used to make Bacillus fuqu, and small and medium scale solid-state fermentation experiments of xiaoqu liquor were carried out to verify the effect of Bacillus on the flavor substances of Xiaoqu liquor. The results showed that the enhanced fermentation with B8 and B13 could significantly reduce the contents of higher alcohols, which were mainly due to the reduction of isoamyl alcohol and isobutanol, and the production of trace n-amyl alcohol. The highest reduction rate for the pilot experiments reached 29.87%, which was lower than the contents of higher alcohols in the commercial finished liquor of Xiaoqu clear flavor liquor. The study provides a method for solving the problem of high content of higher alcohols in Xiaoqu liquor, which is expected to help improve the quality of Xiaoqu liquor.
    19  Screening and Gas Production Capacity Analysis of a Halotolerant Zygosaccharomyces rouxii Strain Isolated from the Swollen Packages of Pixian Broad Bean-chili Paste
    FAN Zhiyi WANG Zeliang ZHANG Qisheng CHEN Gong DENG Weiqin RAN Yalin LI Heng
    2023, 39(2):152-160. DOI: 10.13982/j.mfst.1673-9078.2023.2.0315
    [Abstract](163) [HTML](564) [PDF 55.39 M](1301)
    Abstract:
    Pixian broad bean-chili paste (PBCP) is a traditional condiment in Southwest China, and package swelling is one of the major quality flaws in commercial PBCP. In this study, PBCP and its two semi-finished products, namely broad bean moromi (BM) and pickled chili (PC) paste were collected. Salt contents and water activities (aw) of the samples were determined and the halotolerant microorganisms were isolated. The results showed that the gas-producing samples had relatively lower salt contents and higher aw values. By inoculating the diluted sample into YM broth media containing 12% (m/m) of NaCl, Zygosaccharomyces rouxii, a halotolerant yeast, was isolated. The production of gas could be induced through inoculating the yeast in YM broth medium, or by re-inoculating the yeast in the autoclaved BM, PC and PBCP with salt contents lower than 16% (m/m). The optimal growth conditions for Z. rouxii were 4% (m/m) NaCl, pH 4~6 and 30 ℃, with the gas production being largest at 8% (m/m) NaCl and pH 5~7. Gas production by Z. rouxii was inhibited when the salt content increased in the culture medium or the sterilized samples. Z. rouxii can grow under acidic and high-salt conditions and can produce gas in PBCP, causing package swelling.
    20  Enzymatic Hydrolysis Preparation and Selenium Content Analysis of Antihypertensive Peptides in Selenium-rich Walnut Meal
    LU Aichun SU Jiayi YANG Xun RAN Jiaxin GUO Junbin TANG Dejian QI Meng XIA Zengrun LIANG Xingtang YIN Yanzhen CAO Yong MIAO Jianyin
    2023, 39(2):161-169. DOI: 10.13982/j.mfst.1673-9078.2023.2.0390
    [Abstract](169) [HTML](181) [PDF 53.34 M](1233)
    Abstract:
    Selenium-rich walnut meal was obtained after extracting oil from selenium-rich walnuts, and the inhibition of angiotensin-converting enzyme (ACE) was used as an evaluation marker. The preparation process of ACE-inhibiting peptides in selenium-rich walnut meal was optimized through single-factor experiments and response surface methodology, and the hydrolysate was subjected to ultrafiltration separation, amino acid composition analysis, and selenium quantification. The following conditions were optimal for the preparation process of ACE-inhibiting peptides in selenium-rich walnut meal: enzymatic hydrolysis temperature of 35 ℃, enzymatic hydrolysis time of 2.52 h, pH of 7.5, substrate concentration of 5.39%, and enzyme addition amount of 0.33%. The ACE inhibition rate in the verification test performed under these experimental conditions was 79.13%, which was close to the theoretical value of 78.84%. The optimal hydrolysate was separated using a 3 ku ultrafiltration membrane, and the ACE inhibition activity of the <3 ku ultrafiltration fraction was 77.78% at the concentration of 1 mg/mL. Hydrophobic amino acids accounted for 27.19% of the hydrolysate of ACE-inhibiting peptides in selenium-rich walnut meal, and the selenium content of the hydrolysate and <3 ku ultrafiltration fraction was 0.82 and 1.35 mg/kg, respectively. These findings provide a theoretical basis for research and development on food-derived selenium supplementation products and auxiliary blood pressure-lowering functional foods.
    21  Construction and Fermentation of Efficient Violacein-producing Corynebacterium glutamicum Strains using Low Copy Number Plasmids
    LU Danni HONG Peixiong ZHANG Guochao YE Yanrui
    2023, 39(2):170-179. DOI: 10.13982/j.mfst.1673-9078.2023.2.0389
    [Abstract](146) [HTML](212) [PDF 36.89 M](1567)
    Abstract:
    For high violacein production, a recombinant strain was constructed using Corynebacterium glutamicum [generally recognized as safe (GRAS)] as the host cell. The low copy number plasmid pOK12CG1 was constructed using the replication and partition elements from pTET3, a natural large plasmid of C. glutamicum. The plasmid pOK12CG1, maintained at ~6 copies per genome, was compatible with common plasmids pEC-XK99E and pXMJ19 of C. glutamicum. The violacein synthesis operon (vioABCDE)-carrying plasmid pCGvio was constructed using the low copy number plasmid pOK12CG1 as backbone. Seven violacein-producing recombinant strains were developed using the standard strain of C. glutamicum ATCC 13032 and the deletion strain of the insertion sequence (IS) element as host cells. Initial screening indicated that the recombinant strain ATCC 13032/pCGvio harboring low copy number plasmids produced more violacein (508.24 mg/L) than the recombinant strain ATCC13032/pECvio harboring medium and high copy number plasmids (376.16 mg/L). Violacein production by the IS element deletion recombinant strain ISDM023/pCGvio reached 610.13 mg/L. Fermentation conditions for the recombinant strain ISDM023/pCGvio were further optimized by orthogonal experimental design to optimize three factors: medium volume ratio (VLB:VBHIS), induction time, and IPTG inducer concentration. Under optimal conditions (VLB:VBHIS=1:2, induction time=18 h, and IPTG concentration=0.75 mmol/L) the final production yield of violacein in shake flasks was 1 007.47 mg/L. Herein, the recombinant strain ISDM023/pCGvio of C. glutamicum was successfully constructed, providing a biological system for efficient production of violacein in C. glutamicum and serving as a reference for the efficient production of other high-value compounds.
    22  Differences in Microbial Community and Physicochemical Properties of Different Layers of Fermentative Material in the Pit of the Fourth Round of Jiangxiang Baijiu
    WANG Yurong HOU Qiangchuan TIAN Longxin ZHOU Jiaping LIU Juzhen GUO Zhuang
    2023, 39(2):180-187. DOI: 10.13982/j.mfst.1673-9078.2023.2.0318
    [Abstract](134) [HTML](351) [PDF 55.78 M](1337)
    Abstract:
    In this study, the fourth round of alcoholic fermentative materials at different depths in Jiangxiang Baijiu fermentation pit was taken as the research object. The pure culture technology combined with high-throughput sequencing technique was used to detect the microbial diversity and community structure, meanwhile, the bionics technology and conventional physical and chemical analysis methods were used to determine the sample quality. The results showed that the total numbers of colony, lactic acid bacteria, molds and yeasts in the fourth-round alcoholic fermentative materials at different depths of fermentation pit were in the order of upper > middle > lower, with all the contents higher than 105 CFU/mL. The contents of moisture, reducing sugar, starch and protein as well as acidity differed insignificant (P>0.05). AS for sensory quality, the lower layer alcoholic fermentative material had a stronger fragrance but its bitterness and astringence were greater. The sequencing results indicated a total of 50 bacterial genera and 74 fungal genera in different stratified fermentative material, and their bacterial community compositions were relatively similar, with the absolute dominant bacterial was Lactobacillus (91.59%); The composition of fungi in each layer differed significantly, and their abundance was higher than that of bacteria, with Thermoascus having the highest relative abundance (33.45%). The correlation analysis showed that there were complex biological processes among the microbial communities in the fourth round of alcoholic fermentative material. Compared with the bacteria, the correlation between fungal genera and physico-chemical indexes were more complex. Thermoascus, Rasamsonia and Aspergillus might be the key genera in the fermentation process of alcoholic fermentative material. Comprehensive analysis showed that the microbial composition and flavor of the fermented materials at the middle and lower layers were more unique compared with the fermented materials with different spatial distribution in the same cellar.
    23  Isolation and Screening of Caproic Acid-producing Bacteria from Shedian Laojiu Cellar and Acid Production Conditions
    ZHANG Huifang ZHANG Wanying ZHOU Suo ZHOU Yehao TAN Ying SUN Junke KE Tao HU Fan
    2023, 39(2):188-196. DOI: 10.13982/j.mfst.1673-9078.2023.2.0264
    [Abstract](188) [HTML](149) [PDF 34.70 M](1229)
    Abstract:
    Caproic acid-producing bacteria represent important acid-producing functional bacteria in pit mud. The generated hexanoic acid is the major component of ethyl caproate, which confers the characteristic aroma of Luzhou flavored liquor, and its application in liquor production may improve the aroma and quality. Here, caproic acid-producing bacterial strains were screened and purified from the pit mud and yellow water of different Shedian Laojiu cellars. Through copper sulfate color development and gas chromatographic detection, 96 caproic acid- and butyric acid-producing strains were screened from 118 strains, majority of which were facultative anaerobic bacteria. Moreover, using 16S rRNA sequencing, 10 microbial strains, including Lysinibacillus, Bacillus, Rummeliibacillus, Stenotrophomonas, and Corynebacterium, were identified. Antagonistic confrontation assay of these caproic acid-producing bacteria revealed no antagonistic effects among the 10 strains, indicating the feasibility of mixed culture. When sodium acetate concentration in the medium was increased from 0.5% to 1.0%, the total amount of butyric and caproic acid in the product was the highest. However, the total amount of caproic and butyric acid decreased when sodium acetate concentration exceeded 1.5%. Moreover, 74% of the strains produced more total acid under anaerobic conditions than under aerobic conditions, and 63% of the strains produced more total acid at 30℃ than at 37℃ under anaerobic conditions. During the 18-day fermentation process of five caproic acid-producing strains, bacterial cell concentration gradually increased with culture time. Butyric acid level peaked at the middle of fermentation, followed by a gradual decrease; however, caproic acid accumulated gradually at the later stages of fermentation, with the peak yield reaching 3 g/L.
    24  Structure Characterization and Immunological Activity Evaluation of the Polysaccharides from Salt-processed Morinda officinalis
    LAI Lanyu LUO Zhifeng ZHU Siyang TAO Qian LI Pan DU Bing
    2023, 39(2):197-204. DOI: 10.13982/j.mfst.1673-9078.2023.2.0286
    [Abstract](145) [HTML](186) [PDF 884.49 K](608)
    Abstract:
    In this study the polysaccharides from salt-processed Morinda officinalis SMP was extracted by water extraction and ethanol precipitation, and then purified sequentially by DEAE-52 cellulose and Sephadex-G100 columns, to yield a purified polysaccharide (named SMP-4). Fourier transform infrared spectroscopy, high performance gel permeation chromatography, gas chromatography and nuclear magnetic resonance were employed to characterize the structural properties of SMP-4, and its immunomodulatory activities were examined in Macrophage RAW264.7 through determining its cell proliferation activity and levels of secreted cytokines. The polysaccharide structure analysis showed that SMP-4 consisted of arabinose, galactose, rhamnose, ribose, xylose, glucose and mannose in the molar ratio of 0.55:0.23:0.16:0.03:0.02:0.01:0.01, and had a molecular weight of 1.32×105 u. SMP-4 had six kinds of glycosidic bonds, with →3)-D-linked-Araf-(1→ accounting for the highest proportion (58.35%). The immunological activity analyses showed that the proliferation of RAW264.7 cells was not affected in the concentration range of 15.625~500.000 μg/mL as compared with the control group, whilst the secretions of tumour necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were significantly promoted. At a SMP-4 concentration of 500 μg/mL, the amount of TNF-α secreted by RAW264.7 cells reached the highest level (2 100.00 pg/mL) (P<0.001); At a SMP-4 concentration of 31.25 μg/mL, RAW264.7 cells secreted the highest amount of IL-1β (14.50 pg/mL) (P<0.05). In conclusion, SMP-4 exhibited a good immune activity and has the potential to be a natural immunomodulator.
    25  Changes in Chemical Composition, Antioxidant Activity and Dominant Microbes during the Wine Production with Rosa roxburghii Tratt via Natural Fermentation
    PENG Huan HUANG Zijian WU Tao LIU Liangxiu CHEN Guojun YANG Ximiao LIAO Zhenlin FANG Xiang WANG Jie
    2023, 39(2):205-213. DOI: 10.13982/j.mfst.1673-9078.2023.2.0317
    [Abstract](199) [HTML](241) [PDF 36.15 M](1386)
    Abstract:
    In this study, Rosa roxburghii Tratt was used to produce wine via natural fermentation. The sensory, physico-chemical properties (total acid, pH, reducing sugar and alcohol content), antioxidant components, in vitro antioxidant activity and microbial diversity were examined. The results showed that total acid content increased from 9.20 g/L to 29.69 g/L, pH value increased from 3.26 to 3.50, reducing sugar content decreased from 24.43 g/L to 9.17 g/L, alcohol content increased from 5.72%vol to 9.81%vol, with the total phenolic content (calculated in gallic acid) beng 0.90~5.48 mg/mL, the total flavonoid content (calculated in rutin) beings 0.46~5.43 mg/mL, and the ascorbic acid content being 0.89~3.37 mg/mL during the fermentation. After 23 days of fermentation, Rosa roxburghii Tratt fruit wine had a yellow color, and was clear and transparent, with strong aroma and refreshing taste. The in vitro antioxidant activity analyses showed that the antioxidant capacity of the wine increased firstly and then decreased with the extension of fermentation time, which was consistent with the changing trend of antioxidant components. The changes of antioxidant substances and scavenging rates of three kinds of free radicals indicated that the changes of antioxidant activities during Rosa roxburghii Tratt fermentation were significantly correlated with the changes of the total phenolic, total Flavonoid and ascorbic acid contents. The analysis of microbial diversity showed that Saccharomyces cerevisiae was the dominant fungi in Rosa roxburghii Tratt fermentation, whilst the dominant bacteria were Petiococcus acidilactici and Sphingomonas echinoderma.
    26  Storage Quality of Electron Beam Irradiated Cooked Silver Carp
    LI Meijin HUANG Jiajun CHEN Yanan CAI Jun LI Hailan ZU Xiaoyan
    2023, 39(2):214-220. DOI: 10.13982/j.mfst.1673-9078.2023.2.0292
    [Abstract](136) [HTML](176) [PDF 19.52 M](1689)
    Abstract:
    In this study, cooked silver carp cubes were used as the raw materials, and they were treated with electron beam at 0, 4 or 8 kGy then stored at room temperature and 4 ℃, respectively. By measuring the changes in total viable count (TVC), pH value and thiobarbituric acid (TBA) value, peroxide value (POV), total volatile base nitrogen (TVB-N) value and texture of the silver carp cubes during storage, the effects of different irradiation doses and storage temperatures on cooked silver carp cubes on storage quality of the silver carp cubes. The results showed that the higher the irradiation dose, the better the sterilization effect was, and the sterilization effect for the storage at 4 ℃ was better than that at room temperature. The storage temperature had insignificant effect on the pH value of silver carp cubes, whilst the irradiation at 8 kGy significantly reduced the pH value of fish cubes (P<0.05). The TBA value of silver carp cubes increased with the increase of irradiation dose, but different irradiation doses and different storage temperatures (P>0.05) caused insignificant difference. Electron beam irradiation effectively inhibited the increases of POV value and TVB-N value (P<0.05) compared with the unirradiated group. Texture analyses revealed that at storage temperature of 4 ℃ and irradiation dose of 4 kGy, the sterilization effect could be maintained, while the textural indices (hardness, elasticity, chewiness, etc) of the cooked silver carp cubes being improved. In summary, the cooked silver carp cubes cold be preserved the best at an irradiation dose of 4 kGy and storage temperature of 4 ℃. The above research will provide a theoretical basis for the application of electron beam irradiation in storing and preserving silver carp.
    27  Effects of Electron Beam Irradiation on the Infestation and Quality of Dried Goji (Lycium barbarum)
    CHENG Zhulin WANG Xiaoyu REN Guiping NI Xiaohui YANG Jing HUANG Wenshu
    2023, 39(2):221-228. DOI: 10.13982/j.mfst.1673-9078.2023.2.0383
    [Abstract](174) [HTML](145) [PDF 827.80 K](683)
    Abstract:
    The effects of electron beam irradiation on the quality of dried goji were examined, and the dosage of irradiation as an insecticide was determined. Dried goji was irradiated using an electron irradiation accelerator, and the effects of different doses of irradiation on the rehydration ratio, insect infestation rate, color, reducing sugars, carotenoids, and other physicochemical indicators were evaluated. The rehydration ratio of irradiated dried goji was significantly lower than that of non-irradiated dried goji. Electron beam irradiation treatment significantly inhibited the insect infestation of dried goji, and the higher the irradiation dose, the more significant the inhibitory effect on the infestation rate. At the irradiation doses of 4~8 kGy, no insects appeared in goji until the end of the experiment. At the irradiation dose of 2 kGy, the infestation rate was 0.57% at the end of the experiment, which was significantly lower than that in the non-irradiated dried goji (0.97%). At doses of 8 kGy or higher, the L*, a*, and b* values of dried goji were significantly lower than those of non-irradiated dried goji. Irradiation significantly increased the contents of reducing sugars and polysaccharides in dried goji but did not significantly affect soluble sugars and free amino acids. The carotenoid and flavonoid content of dried goji decreased significantly at irradiation doses of 8 kGy or higher. In summary, the electron beam irradiation dose of dried goji should be in the range of 4~8 kGy. Doses in this range can effectively inhibit the infestation of dried goji and allow for maintaining their original edible quality to the greatest possible extent.
    28  Inhibitory Effect of Curcumin-mediated Photodynamic Technology on the Mycelial Growth and Patulin Secretion of Penicillium expansum
    PANG Jialei ZHANG Fang
    2023, 39(2):229-234. DOI: 10.13982/j.mfst.1673-9078.2023.2.0334
    [Abstract](165) [HTML](156) [PDF 49.32 M](1388)
    Abstract:
    The effects of curcumin-mediated photodynamic technology (CUR-PDT) on the mycelial growth and patulin (PAT) secretion of Penicillium expansum were investigated. Mycelia of P. expansum grew vigorously after 5 days of solid culture, and PAT secretion increased 6.5 times. Short-term treatment with curcumin slightly inhibited the mycelial growth and PAT secretion. At a low dose of 0.275 W/cm2, 50~300 μmol/L curcumin significantly reduced the colony diameter (0.84 cm) and mycelial weight (68.37 mg) of P. expansum in solid culture, and the inhibition rate of PAT secretion was 83.85%. Following the inoculation of Red Fuji apples with PDT-treated mycelia and culture for 8 days, the inhibition rate of lesion diameter was positively correlated with the concentration of curcumin, with the maximum inhibition rate of 49.38%. The distribution of PAT in the fruit was closely correlated with the mycelial expansion ability, and the lesion proportion and PAT content were 0.09% and 18.02% respectively. Therefore, CUR-PDT can significantly inhibit the mycelial growth and PAT secretion of P. expansum.
    29  Effects of Different Packaging Methods on the Quality of Fresh Shiitake Mushrooms Sold in Farmers’ Markets
    ZHAI Mengjie DU Minru LIN Hui GU Fengju LI Yanzeng LI Weipeng LI Chen WANG Jie
    2023, 39(2):235-242. DOI: 10.13982/j.mfst.1673-9078.2023.2.0385
    [Abstract](165) [HTML](157) [PDF 5.76 M](480)
    Abstract:
    The effects of four packaging methods on the quality of fresh shiitake mushrooms (appearance, texture, gas concentration, cell membrane integrity, nutrient content, antioxidant enzymatic activity, and antioxidant content) sold in farmers’ markets were studied. Compared with those in other treatments, shiitake mushrooms in PE packaging showed better appearance quality, and PE packaging improved firmness (3.50%), catalase (CAT) activity (1.24%), and superoxide dismutase (SOD) activity (49.08%) during the early storage period. On the third day of storage, the rate of weight loss of shiitake mushrooms in PE packaging increased by only 2.87%; meanwhile, the contents of total sugars and malondialdehyde (MDA) decreased by respectively 19.32% and 20.11%, whereas the contents of total phenols and flavonoids, which are antioxidant substances, increased by respectively 2.16 times and 9.92 times. In conclusion, PE packaging exhibited the best overall effect and maintained the quality of fresh shiitake mushrooms by delaying the consumption of total sugars and decline of firmness, inhibiting the rate of weight loss and MDA production, and improving the antioxidant potential. These findings offer data support for maintaining the quality and reducing the economic losses of fresh shiitake mushrooms in the process of market circulation.
    30  Changes in the Quality of Crayfish Meat during Frozen Storage
    SANG Yanfei YANG Li QIAN Xinping JIANG Shaotong LU Jianfeng LIN Lin
    2023, 39(2):243-252. DOI: 10.13982/j.mfst.1673-9078.2023.2.0329
    [Abstract](169) [HTML](146) [PDF 66.20 M](494)
    Abstract:
    In order to study the effects of frozen storage on the quality of crayfish meat, live (FX group) and steamed (FS group) crayfish were used as the research objects. Changes in the total volatile basic nitrogen (TVB-N), texture, microstructure, electrophoresis, sulfhydryl groups, thiobarbituric acid reactive substances (TBARS), carbonyl groups, surface hydrophobicity, and infrared spectroscopic properties during the frozen storage of samples in both groups were investigated. During frozen storage, TVB-N gradually increased to 22.93 and 11.90 mg/100 g in the FX and FS groups, respectively, although neither value exceeded the upper safety limit (30 mg/100 g). Similarly, the carbonyl content of shrimp meat increased to 0.094 and 0.042 μmol/g in the FX and FS groups, respectively. In the FX group, surface hydrophobicity increased initially and then decreased, and the sulfhydryl content decreased by 41%. Meanwhile, in the FS group, sulfhydryl content fluctuated in the range of 0.26~0.81 μmol/g. The TBARS value increased initially and then decreased. The texture of crayfish meat became loose during frozen storage, with shortened sarcomeres and obvious degradation of Z- and M-lines. The myosin bands were substantially degraded, and the α-helix and random coil structures of muscle proteins were transformed to β structures. Therefore, steaming pretreatment is beneficial to maintain the quality of crayfish meat during frozen storage.
    31  Effects of Pre-hydrolysis on the Gastrointestinal Digestion and Immunomodulatory Activity of Maca Proteins
    PAN Leiman ZHANG Yi HE Ping ZHANG Yizhe LIN Zhengli YANG Jinxi ZHANG Lina WU Hui ZHANG Mengmeng
    2023, 39(2):253-263. DOI: 10.13982/j.mfst.1673-9078.2023.2.0395
    [Abstract](137) [HTML](146) [PDF 81.66 M](539)
    Abstract:
    The effects of pre-hydrolysis using different proteases on the degree of gastrointestinal digestion of maca proteins and the immunomodulatory activity of their digestion products were explored. Dispase, alcalase, papain, bromelaine, and protamex were selected to pre-hydrolyze maca proteins, and the final five digestion products after subsequent in vitro simulated gastrointestinal digestion were obtained. The yield, degree of hydrolysis, soluble peptide content, and free amino acid content of the digested products were determined using the TNBS method and Folin-Ciocâlteu assay. Mouse macrophages RAW 264.7 were used as the model to explore the immunomodulatory activity of the digestion products. Following pre-hydrolysis with the five proteases, the degree of hydrolysis, soluble peptide content, and free amino acids content of the maca protein digestion products were augmented by respectively 35%, 5%~16% and 37%~92%, while no significant inhibitory effect of their promotion on the secretion of TNF-α by macrophages was observed. The digestion products obtained via bromelain pre-hydrolysis showed the highest immunomodulatory activity and comprised 15 154 peptides, with a molecular weight of <3 000 u. Among the top 10 peptides with the strongest interactions with TLR2 and TLR4 predicted based on activity scores and molecular docking, NPYPFFGFSI exhibited the highest immunomodulatory activity. HOMO analysis revealed that the active site of this peptide is located at tyrosine. Therefore, the digestibility of maca proteins can be improved via pre-hydrolysis without compromising their immunomodulatory activity.
    32  Effect of Ultra-low Temperature Double Spiral Quick Freezing on the Processing Quality of Pork Longissimus Dorsi Muscle
    WANG Yijiao David S. Casey SHEN Ting ZHANG Wangang
    2023, 39(2):264-271. DOI: 10.13982/j.mfst.1673-9078.2023.2.0324
    [Abstract](133) [HTML](339) [PDF 6.50 M](525)
    Abstract:
    The effects of ultra-low temperature double spiral quick freezing (UTDSQF) on the processing quality of raw pork were investigated. The longissimus dorsi muscles of pigs with a pH value within the normal range at 24 h after slaughter were used as the research object. The muscle samples were subjected to UTDSQF or conventional air-blast freezing (AF). After freezing at -20 ℃ for 90 days, the samples were thawed at 4 ℃ and processed into emulsified sausages to determine the effects of both freezing methods on the physicochemical, textural, and sensory qualities. The different freezing methods of raw pork did not significantly affect the pH of emulsified sausages (P>0.05). Compared with AF, UTDSQF reduced the TBARS value and cooking loss by respectively 26.67% and 31.25% (P<0.05). The L* and a* values increased significantly (P<0.05), but the b* value decreased significantly (P<0.05). Hardness, chewiness, resilience, and cohesiveness of the UTDSQF-treated sausages were significantly improved (P<0.05), although there was no significant effect on elasticity (P>0.05). Furthermore, the UTDSQF treatment of raw pork significantly improved the sensory score of emulsified sausages (P<0.05). Therefore, UTDSQF can improve the overall processing quality of raw meat.
    33  Effects of Different Comminution Methods Used during Aqueous Enzymatic Extraction on the Quality of Walnut Oil
    ZHAO Huibo DENG Jiankang LU Min QI Bing DAI Shengxing WU Yanbing WANG Ying WANG Yaning XIA Junxia
    2023, 39(2):272-279. DOI: 10.13982/j.mfst.1673-9078.2023.2.0311
    [Abstract](151) [HTML](229) [PDF 26.35 M](582)
    Abstract:
    In this research, walnut kernel was processed by six types of comminution methods, and walnut oil was prepared by aqueous enzymatic extraction (AEE). The effects of different types of comminution methods on walnut kernel particle size, oil yield, residual protein and oil acid value, peroxide value, fatty acid composition and volatile composition were studied. The results show that the oil yield was 54% after anhydrous colloid grinding and two 3-roll grindings. Walnut oil D (anhydrous colloid grinding and two 3-roll gridings) had higher relative fatty acid content and lower unsaturated fat content. The volatile components of walnut oil were analyzed by headspace-solid phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). A total of 56 volatile components were identified. The relative high proportions of aldehydes, esters and hydrocarbons were revealed by the cluster heatmap. Taken together, the combination of anhydrous colloid grinding and one 3-roll grinding was better than other methods, allowing continuous crushing of walnut and the production of good quality walnut oil. The extraction of walnut oil by the aqueous enzymatic method requires the reduction of the particle size of raw material and the emulsification during crushing. This study provides a support for extracting walnut oil by aqueous enzymatic method and lay a foundation for accurate and moderate processing of walnut oil.
    34  Comparison on the Quality of Black Tea Prepared by Different Freezing and Shaking Processes
    XIA Yimin WANG Jinjin YUAN Haibo
    2023, 39(2):280-289. DOI: 10.13982/j.mfst.1673-9078.2023.2.0261
    [Abstract](175) [HTML](172) [PDF 27.99 M](606)
    Abstract:
    In this study, the effects of the freezing treatment prior to withering and the combination of freezing treatment with one, two and three times shaking after withering on the formation of black tea quality were analyzed, and the optimal process conditions were determined. The results showed that the new process could significantly increase the contents of gallic acid, polyphenols, theaflavins and flavonoid glycosides in tea (P<0.05), meanwhile, the freezing treatment could significantly reduce the soluble sugar content (P<0.05) (decreased by 10.73%), and the combination of freezing and shaking could significantly reduce the theabrownine content (P<0.05) with an average decrease of 6.77%. Compared with the freezing treatment, the combination of freezing and shaking significantly reduced the content of tea polyphenols (P<0.05) with an average decrease of 3.55%, whilst increased significantly the theaflavin content (P<0.05) with an average increase of 18.06%. Among all the treatments, he combination of freezing and twice shaking led to relatively high contents of amino acids, theaflavins and cis-caproate-3-hexen ester, and relatively low contents of gallic acid, catechins, flavonoid glycosides, theabrownins and 3-octen-2-one. In the resulting black tea had a bright orange yellow, sweet aroma, mellow quality, with its total sensory score being 91.30, which was 2.40 and 2.60 points higher than the score of the control and the tea subjected to the freezing treatment, respectively. In conclusion, the combination of freezing and twice shaking was the best new process.
    35  Comparative Analysis of the Quality of Original Mulberry Brandy Prepared by Different Distillation Processes
    TIAN Shulin ZHENG Shenghai YANG Kailong QU Du DU Hong XU Shengdong FAN Rong CHEN Rangfang HAN Baolin
    2023, 39(2):290-296. DOI: 10.13982/j.mfst.1673-9078.2023.2.0186
    [Abstract](132) [HTML](170) [PDF 16.11 M](417)
    Abstract:
    In this research, mulberry brandy fermented wine was used as the raw material, which was distilled by secondary distillation. The alcoholic contents, methanol contents and high alcohol contents of the two distillates were determined separately. On this basis, the effects of different combinations of second distillation on the quality of original mulberry brandy wine were studied through sensory evaluation and gas chromatography-mass spectrometry (GC-MS) analyses, in order to determine the optimal distillation process for mulberry brandy. The results showed that the alcohol content and the contents of methanol and higher alcohols decreased gradually with the distillation time during the two distillation processes. Taking the volatile flavor substances, methanol and high alcohol contents, and sensory scoring as evaluation indices, among the four different distillate combinations, the comprehensive evaluation score of the C3 combination (3~7 segments of the distillate) was the highest. The C3 combination had 59.5%vol alcohol, 170.94 mg/L methanol and 745.26 mg/L high-alcohol content, and was clear and transparent with harmonious fragrance and distinct characteristics. The best process for the original mulberry brandy was finally determined to be the C3 combination: two distillations were performed, the second distillate was 68.75% of the first distillate, and sections 3~7 of the second distillation were extracted. The research is expected to lay a foundation for the brewing of high quality mulberry brandy.
    36  The Role of γ-Aminobutyric Acid Shunt in Cronobacter sakazakii Resistance to Desiccation
    LI Li CAO Yifang ZHANG Yan XIAO Xinglong
    2023, 39(2):297-302. DOI: 10.13982/j.mfst.1673-9078.2023.2.0375
    [Abstract](131) [HTML](165) [PDF 3.54 M](425)
    Abstract:
    Cronobacter sakazakii is a food-borne pathogen commonly found in powdered infant formula (PIF). γ-aminobutyric acid (GABA) is a non-protein amino acid widely present in various organisms. 4-aminobutyrate transaminase, encoded by gabT, is a key enzyme in the GABA shunt. This study constructed a gabT gene knockout strain (ΔgabT) of Cronobacter sakazakii ATCC 29544 to investigate the effect of the GABA shunt controlled by gabT on the resistance of Cronobacter sakazakii to desiccation. The results showed that the survival rate of ΔgabT under one-week desiccation stress was 28.64 %, significantly higher than that of the wild-type strain (WT) which was 1.57%. In addition, GABA accumulation in ΔgabT was higher than that in WT, and was positively correlated with survival rate, suggesting that GABA accumulation can help Cronobacter sakazakii resist desiccation stress. The results of scanning electron microscopy also showed that the ΔgabT strain had relatively intact cell membrane morphology, further confirming that GABA accumulation contributes to Cronobacter sakazakii survival under the stress of dry environments. This study lays a foundation for the targeted inhibition of Cronobacter sakazakii based on its GABA shunt and provides new directions for research into prevention and control of Cronobacter sakazakii.
    37  Simultaneous Quantitative Analysis of Plant Metabolites in Different Parts of Perilla (Perilla frutescens) by HPLC
    ZHANG Hongyu WANG Dan WU Chunjian HOU Tianyu ZHAO Yana ZHANG Zhijun LEI Nana LI He
    2023, 39(2):303-312. DOI: 10.13982/j.mfst.1673-9078.2023.2.0296
    [Abstract](247) [HTML](211) [PDF 73.34 M](549)
    Abstract:
    This study aims to establish an analytical method for the simultaneous detection of metabolites in different parts of Chinese Perilla frutescens. Identification of the types and concentrations of metabolites will provide a scientific basis for efficient utilization of P. frutescens compounds. The 19 plant metabolites in the leaves, seed shells and stems of Chinese perilla (Perilla frutescens) were analyzed by solvent extraction combined with high-performance liquid chromatography technique. Results indicated that the 19 compounds were completely separated within 55 minutes with good peak shape and separation, a limit of detection (0.05~0.40 mg/L), correlation coefficients (R2) over 0.997 6, recoveries of 61.65%~111.42%, and precision of 1.02%~17.20%. This method was used to analyze 12 kinds of perilla samples, and it was found that the contents of 19 compounds in different parts of perilla varied significantly in the range of 0.04~24.05 mg/g. Two phenolic acids and five flavonoids and flavonoid glycosides were detected in the 12 samples. Luteolin was detected in perilla shells and stalks, with the contents with the contents ranging between 0.05 and 0.18 mg/g; the decreasing order of compound content in perilla leaves was rosmarinic acid > scutellarin > apigenin-7-O-glucoside. In addition, two furan ring derivatives, HMF and furfural, were detected for the first time in the perilla shells of this study. The method is simple and efficient, and the instrumental requirement is low, thus, is suitable for quantitative determination of metabolites in Perilla samples and other types of plant samples.
    38  Determination of Elements and Stable Isotope Ratios in Cherry Kernel Shells and Analysis of the Geographical Origin of Cherries
    HOU Jianbo HONG Deng ZHANG Mingzhe ZHANG Xiaofeng XIE Wen ZHU Zelong SHI Yingzhu ZHANG Wenhua
    2023, 39(2):313-318. DOI: 10.13982/j.mfst.1673-9078.2023.2.0327
    [Abstract](108) [HTML](151) [PDF 6.20 M](632)
    Abstract:
    Inductively coupled plasma-mass spectrometry (ICP-MS) and elemental analyzer-isotope ratio mass spectrometry (EA-IRMS) were used to determine the contents of 37 elements and stable isotope ratios of hydrogen, oxygen, carbon, and nitrogen (δ2H, δ18O, δ13C, and δ15N, respectively) in the kernel shells of cherries and applied to identify their geographical origin. After removing the pulp and kernels of cherries, the kernel shells were dried and ground to a powder. After microwave digestion, the element contents of the kernel shell powder were determined using ICP-MS. Moreover, δ2H, δ18O, δ13C, and δ15N of the kernel shell powder were determined using EA-IRMS. The elemental contents and stable isotope ratios of cherry samples from Australia, the United States of America, New Zealand, Chile, and China were evaluated. Multivariate analyses of elements, including analysis of variance, Duncan’s multiple comparison, correlation analysis, principal component analysis, cluster analysis, and discriminant analysis, were performed using the relevant SPSS packages to attribute the geographical origin of the cherries. Combined indicators of Mn, Co, Rb, Sr, Ba, and δ2H were the most effective in tracing cherries to their geographical origin, with an overall cross-validation accuracy of 92.1%.
    39  Element Composition Analysis and Heavy Metal Safety Evaluation of Different Varieties of Ganoderma lucidum Fruit Bodies and Their Spore Powders
    PAN Shaoxiang ZHAO Cen YAN Xinhuan LI Hongxiao LIU Xuemei SONG Ye WU Maoyu MA Chao XIONG Yongxing ZHENG Xiaodong
    2023, 39(2):319-324. DOI: 10.13982/j.mfst.1673-9078.2023.2.0254
    [Abstract](127) [HTML](232) [PDF 2.15 M](516)
    Abstract:
    The contents of elements and heavy metals in the fruit body, spore powder and cell wall -broken spore powder of different Ganoderma lucidum (G. lucidum) varieties cultivated mainly in the west of Shandong province of China were determined by inductively coupled plasma mass spectrometry (ICP-MS). Element composition analysis and safety evaluation of heavy metals were conducted. The results of elemental composition analysis showed that the contents of most elements in the fruit bodies of G. lucidum of Zangzhi and Xiangxueshi were significantly higher than those of Chizhi, Meizhi and Xindapian samples, especially the 9 essential microelements for the human body such as Fe, Cu, Zn, Mn, Se, B, V, Co and Sr. The content of Fe, Zn and Se in the fruit body of G. lucidum reached 234 mg/kg, 57.9 mg/kg and 0.309 mg/kg, respectively. The content of Sr in Xiangxueshi G. lucidum was the highest, reaching 3.60 mg/kg. The element nutritional qualities of Chizhi powder and ganodermabasswood powder were higher than those of Meizhi powder and Xindapian powder. The selenium contents of Chizhi spore powder and Ganoderma basswood powder were 3 times and 2 times, respectively, that of Meizhi powder. Heavy metal safety analysis results show that the Pb and Hg contents of the 5 kinds of G. lucidum and 4 kinds of spore powder were far lower than those of the related standards, thus, the safety was higher. The As element contents of Zangzhi, Xiangquezhi and Xindapian spore powders were high, indicating certain safety risk. The contents of As, Pb, Cd, Cr, Cu and Ni metal elements in the spore powders increased in the process of sporoderm breaking, thus, the sporoderm breaking process may bring in certain safety risk to Ganoderma lucidum spore powder.
    40  Applications and Prospects of Micro-Nano Gas Bubbles in the Food Industry
    ZHANG Zhihong ZHANG Lingzhi GAO Xianli WANG Bo WANG Mansheng MA Haile
    2023, 39(2):325-331. DOI: 10.13982/j.mfst.1673-9078.2023.2.0380
    [Abstract](245) [HTML](299) [PDF 19.96 M](567)
    Abstract:
    Micro-nano gas bubbles are micro-bubble particles with diameters ranging from tens of nanometers to tens of micrometers, formed through dispersing gas in a solution. Compared with ordinary bubbles (≥600 μm), micro-nano gas bubbles, owing to their smaller size, are more readily affected by the Brownian motion of aqueous molecules. They are characterized by physicochemical properties, such as strong stability, long presence in solution, rich negative charges at the gas-liquid interface, and free radical formation during bubble collapse. Therefore, micro-nano gas bubbles are widely used in environmental pollution control, agricultural planting, material science, and medicine. However, the application of micro-nano gas bubbles in the food industry remains at the explorative stage. Here, the formation process, preparation method, bubble characteristics, and current applications of this technology in the food industry are described, and the development direction and application prospects of micro-nano gas bubbles in food are explored to promote further research and development of their applications in the food industry.

    Editor in chief:李琳

    Inauguration:现代食品科技

    International standard number:ISSN 1673-9078

    Unified domestic issue:CN 44-1620/TS

    Domestic postal code:46-349

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