Volume 38,Issue 4,2022 Table of Contents

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  • 1  In Vitro Antioxidant Activity of Fucoidan-containing Sulfated Polysaccharide and Its Prolongation of the Lifespan of Caenorhabditis elegans
    WANG Meng GUAN Siyu YU Jie XUE Jin’ai DI Jianbing WANG Yu JIANG Jinju CUI Hongli LI Runzhi
    2022, 38(4):1-9. DOI: 10.13982/j.mfst.1673-9078.2022.4.0629
    [Abstract](324) [HTML](1222) [PDF 916.03 K](609)
    Abstract:
    The in vitro antioxidant effect of fucoidan-containing polysaccharide (FSP) was investigated. Taking Caenorhabditis elegans (C. elegans) as the model animal, an acute oxidative stress model was established using the oxidizing agent, H2O2. The effects of FSP on lifespan, mobility, age pigment and oxidative stress indices were determined. The research showed that FSP exhibited an ability to scavenge hydroxyl radicals and this effect was in dose-dependent; FSP (50, 100 and 200 μg/mL) prolonged the lifespan of C. elegans in a dose-dependent manner (by 7.24%, 26.70% and 48.42%, respectively); FSP significantly increased the motility of nematodes, with the motility of the 200 μg/mL FSP treatment group reaching 73.50%, and the accumulation of lipofuscin and the degree of apoptosis decreased; Under 0.1% H2O2-induced oxidative stress, the treatment with 200 μg/mL FSP enhanced the antioxidant capacity through increasing the activities of antioxidant enzymes, SOD and CAT; The qRT-PCR results showed that FSP influenced the lifespan of nematodes through regulating the expression of related genes in the Insulin/IGF-1 signaling pathway. In summary, FSP could exert protective effects on oxidative damage in nematodes through extending the lifespan of C. elegans, enhancing its mobility, promoting the activities of antioxidant enzymes, regulating the expression of key genes and inhibiting the level of oxidative stress.
    2  Comparison of the Anti-diabetic Effects of the Crude Polysaccharides from Iron Stick Yam and Bergamot Yam
    CAI Yu ZHI Fei CHEN Yunzhong LIU Simin YAN Chunchao WAN Weixi SHE Yaru ZENG Min
    2022, 38(4):10-18. DOI: 10.13982/j.mfst.1673-9078.2022.4.0519
    [Abstract](278) [HTML](316) [PDF 752.33 K](759)
    Abstract:
    In order to compare the antidiabetic effects of the crude polysaccharides of iron stick yam and bergamot yam, the scavenging effects of the two kinds of crude polysaccharides on DPPH and PTIO free radicals, the inhibitory effects on α-glucosidase, and the hypoglycemic and hypolipidemic effects were determined. Type 2 diabetes model rats were established, and metformin was used as the positive control drug. The EC50 values (µg/mL) of the two kinds of crude polysaccharides were 35.38 and 16.19 for DPPH radical scavenging (p<0.01), and 7869.17 and 8335.78 for PTIO radical scavenging (p<0.01), respectively, with the inhibition of α-glucosidase (18.85% and 15.73%, respectively) being insignificantly different (p>0.05). Compared with the model group, the iron stick yam group had decreased FBG, TC and LDL-C levels (by 24.38%, 28.44% and 44.68%, respectively; p<0.05, p<0.01), increased liver glycogen content (by 21.83%; p<0.05), and increased CAT and GSH-Px levels (by 363.36% and 43.67%, respectively; p<0.01). The bergamot yam group had decreased FBG, TG, TC and LDL-C levels (by 25.62%, 28.52%, 22.79% and 44.73%, respectively; p<0.05, p<0.01), and increased SOD and CAT levels (by 7.22% and 69.76%, respectively; p<0.05). The results indicated that the two crude polysaccharides had different antidiabetic mechanisms. The crude polysaccharide from iron stick yam exhibited more advantages in scavenging PTIO radicals, reducing hepatic glycogenolysis, and enhancing CAT and GSH-Px activities, whilst the crude polysaccharide from bergamot yam was more effective in scavenging DPPH radicals, reducing TG content, and enhancing SOD and CAT activities.
    3  Tea Seed Saponin Ameliorated Lipid Metabolism and Oxidative Stress in High Fat Diet Fed Mice
    CAO Wengjing LIU Shuang SU Yanmin LI Jiali QIN Huishan ZENG Zhen SONG Jiale
    2022, 38(4):19-27. DOI: 10.13982/j.mfst.1673-9078.2022.4.0731
    [Abstract](182) [HTML](298) [PDF 1.67 M](488)
    Abstract:
    In this study, the effect of tea seed saponin on lipid metabolism and oxidative stress caused by high-fat diet in mice was investigated. The food intake and body weight changes of mice were observed. The levels of serum superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), total cholesterol (TC), triglycerides (TG), low density lipoprotein (LDL-C) and high density lipoprotein (HDL-C) were determined by relevant commercial kits. The hepatic steatosis levels were observed by H&E staining assay. The hepatic protein levels of AMPK, p-AMPK, SIRT1, PGC-1α and PPAR-γ were detected by western blot assay. The experimental results showed that TSS treatment significantly reduced the body weight, and organ index of epididymal fat, perirenal fat and abdominal fat in HFD mice (p<0.05). The serum levels of TC (37.83%), TG (64.77%) and LDL-C (76.83%) were reduced in high dose of TSS treated mice than that in HFD mice. TSS treatment significantly reduced the hepatic steatosis and promoted the protein levels of p-AMPK (3.92 times, 4.95 times and 6.63 times), SIRT1 (0.86 times, 1.36 times and 1.77 times), PGC-1α (3.10 times, 3.11 times and 4.33 times) and PPAR-γ (2.83 times, 4.27 time and 5.51 times) in HFD mice (p<0.05). After treated by TSS, serum levels of SOD and GSH were increased in HFD mice. In addition, serum MDA was also decreased in TSS treated HFD mice. Taken together, our results suggested that TSS reduced fat accumulation, improved the lipid metabolic disorder and attenuated the oxidative stress status in mice induced by HFD.
    4  Anti-infection and Anti-inflammatory Effects of Cranberry Products on Zebrafish
    FU Shijie LI Aimin WU Xiaolei LI Zijie
    2022, 38(4):28-33. DOI: 10.13982/j.mfst.1673-9078.2022.4.0847
    [Abstract](146) [HTML](168) [PDF 910.34 K](455)
    Abstract:
    The antibacterial and anti-inflammatory effects of four types of cranberry products, including cranberry juice powder, cranberry whole fruit powder, cranberry extract powder (MYMFC), and commercially available concentrated cranberry extract (high-strength cranberry) was evaluate. Sanjin tablets, a commercially available Chinese medicine, was used as a positive control. First, the No Observed Adverse Effect Levels (NOAEL) of the Sanjin tablets and four cranberry products on zebrafish were determined as 1000 and 2000 μg/mL, respectively. Next, low, medium, and high concentrations of these products were used for feeding experiments using transgenic zebrafish with neutrophil fluorescence infected with P fimbriae-positive Escherichia. coli. The antibacterial and anti-inflammatory effects of the cranberry products were determined by measuring the average fluorescence intensity of bacteria in the intestinal lumen and number of neutrophils. The results showed that Sanjin tablets and the four tested cranberry products at high concentrations had extremely significant inhibitory effects (***p < 0.001) on bacterial infection and inflammation in zebrafish, with MYMFC showing the strongest effect. At high concentrations, its bacterial inhibition rate reaches 60.38%, and the inflammation inhibition rate reached 51.16%; these values exceed those of Sanjin tablets (53.84% and 46.51%, respectively). These results demonstrate that cranberry products are safe and have strong antibacterial and anti-inflammatory effects, with MYMFC showing superior antibacterial and anti-inflammatory capabilities compared to those of Sanjin tablets.
    5  Preparation and Stability Characterization of Gliadin Hydrolysates from Bitter Almond Kernels with α-Glucosidase Inhibitory Activity
    WU Anqi YI Chunmin ZHAO Xuexu MA Peixuan WU Rui SHAN Liang
    2022, 38(4):34-42. DOI: 10.13982/j.mfst.1673-9078.2022.4.0874
    [Abstract](197) [HTML](177) [PDF 3.97 M](587)
    Abstract:
    The optimal protease for preparing bitter almond kernel gliadin-based α-glycosidase inhibitory hydrolysates (AGIH) was determined according to the inhibition rate and degree of hydrolysis. Based on the α-glycosidase inhibition rate, optimal process parameters for AGIH preparation, including the substrate concentration required, amounts of enzymes added, pH, temperature, and duration, were examined in single-factor and response surface experiments. The physical stability (against pH and temperature) and gastrointestinal stability of the resulting AGIH were characterized. Papain was found to be the optimal protease; the optimal conditions were as follows: substrate concentration of 4.0% (m/V), enzyme addition of 6000 U/g, initial pH of 7.0, hydrolysis temperature of 55 ℃, and reaction time of 6 h. AGIH prepared under these conditions showed an α-glucosidase inhibition rate of 18.10% and IC50 value of 17.66 mg/mL, and was stable under relatively high temperature, extreme pH, and gastrointestinal digestion conditions. This study provides a foundation for developing bitter almond kernel gliadin-based bioactive hydrolysates.
    6  In Vitro Antioxidant Activity of the Metabolites of Walnut Endophytic Fungus LTS-6-6 and Their Effects on Oil Oxidation Stability
    ZHAO Chendong PANG Junqian ZHAO Xindan HAO Yuanru ZHAI Meizhi
    2022, 38(4):43-51. DOI: 10.13982/j.mfst.1673-9078.2022.4.0596
    [Abstract](221) [HTML](340) [PDF 737.31 K](478)
    Abstract:
    Taking the walnut endophytic fungus LTS-6-6 as the research object, the in vitro antioxidant activities of different-polarity extracts of its metabolites were determined by the ABTS free radical scavenging method, the hydroxyl free radical scavenging method and the Fe3+ reduction ability assay; The Schaal oven method was used to determine the effect of adding highly active extracts on the oxidation stability of rapeseed oil using the peroxide value and acid value of oil as indicators. The research results showed that among the four extracts, ethyl acetate extract (EAE) showed a stronger antioxidant activity, and exhibited strong scavenging abilities for ABTS radical and hydroxyl radical (scavenging rate ≥ 80%). The clearance rate of EAE was higher (by 71.39%) than that of petroleum ether extract (PEE). The contents of total phenols and flavonoids in EAE were 643.71 mg gallic acid/g and 102.05 mg rutin/g. The peroxide value and acid value of the rapeseed oil treated with 0.02% EAE and stored at 70 ℃ for 8 days were 5.88 mmol/kg and 0.85 mg/g, respectively. As predicted by the Arrhenius formula, the rapeseed oil with 0.02% EAE had a storage period of 256 days at 20 ℃, which was 192 days longer than that of the blank group, indicating that the addition of EAE effectively inhibited the oxidative rancidity of rapeseed oil.
    7  Preparation Process Optimization for the Antioxidant Peptide from Pinctada martensii Meat and Its Tyrosinase Inhibitory Activity
    HUANG Pantian CHEN Bingbing SHEN Jinpeng HUANG Wen XIA Zhen LI Yingkun WANG Xianghua YU Yan CAO Yong MIAO Jianyin
    2022, 38(4):52-61. DOI: 10.13982/j.mfst.1673-9078.2022.4.0468
    [Abstract](275) [HTML](256) [PDF 5.45 M](618)
    Abstract:
    In this study, taking Pinctada martensii meat as the research object and the ABTS free radical scavenging rate as the evaluation index, the preparation process of antioxidative peptides was optimized by the single factor experiments and response surface method. The antioxidant ability and tyrosine inhibitory ability of the lyophilized powder of the enzymatic hydrolyzate were studied. The results showed that the optimal enzymatic hydrolysis conditions were: 50 ℃, pH 7.25, 3 h, material-to-liquid ratio 1:1, enzyme-to-substrate ratio 0.4%. The ABTS free radical scavenging rate was 98.48%. The antioxidant peptide obtained from Pinctada martensii under these conditions showed excellent ABTS free radical scavenging effect (IC50 0.57 mg/mL) and Fe2+ chelating activity (IC50 6.89 mg/mL), and good ORAC value (601.38 μmol TE/g freeze-dried powder). Meanwhile, the antioxidant peptide from Pinctada martensii meat exhibited significant inhibitory effects against tyrosine monophenolase (IC50 0.37 mg/mL) and tyrosine diphenolase (IC50 20.27 mg/mL), indicating that it also has an application potential in the field of skin whitening and skin care. The results of this study will provide reference and basic data for the application of Pinctada martensii in the field s of anti-oxidative, anti-aging, whitening and skin care functional foods and cosmetics.
    8  Analysis and Comparison of Peanut Allergen Proteins Ara h2, Ara h3 and Sesame Protein 2S albumin in Pure Sesame Paste
    REN Xiu WANG Yaping BAI Jichao ZHOU Wei ZHANG Xiaodong CHEN Yiwen CUI Shenghui LIN Lan
    2022, 38(4):62-68. DOI: 10.13982/j.mfst.1673-9078.2022.4.0487
    [Abstract](191) [HTML](183) [PDF 811.87 K](538)
    Abstract:
    In this study, real-time PCR(RT-PCR)and enzyme-linked immunosorbent assay (ELISA) were used to detect peanut allergenic proteins Ara h2, Ara h3 and sesame protein 2S albumin in pure sesame paste. The specificity and reproducibility of the two methods were shown to be good. After verification, real-time PCR was used to detect the sesame protein 2S Albumin gene and peanut allergen Ara h2 gene, and ELISA was used to detect the peanut allergen Ara h3 protein. The 90 batches of samples detected by real-time PCR test contained sesame components (with CT value ranging from 22.00 to 33.80), and among which 45 batches of samples contained peanut components (CT value: 25.60~38.60). Besides these 45 batches s, ELISA also allowed the detection of peanut components in another 38 batches. By comparing and analyzing the results of the two methods and through sensitivity analysis research, it was found that the ELISA method had a lower detection limit. Although the results of the two methods were different, they were not contradictory, with both suitable for the detection of peanut sources in pure sesame samples. The real-time PCR method is appropriate for the samples with a high peanut content, whilst the ELISA method can be selected for samples with a low peanut content or unpredictable samples. At present, there are no relevant standards for the detection of allergens in sesame paste. This study provides reliable data for basic research, and is the results suggest that the regulatory authorities should establish relevant methods as soon as possible to supervise market behavior and ensure people's dietary safety.
    9  Rheological Properties of Dextran/Faba Bean Protein Composite Gel
    TANG Huihua CHEN Junfei LIU Biqin WANG Xinrui SHI Qiao LI Hong
    2022, 38(4):69-75. DOI: 10.13982/j.mfst.1673-9078.2022.4.0797
    [Abstract](234) [HTML](205) [PDF 1.04 M](454)
    Abstract:
    In order to study the effect of lactic acid bacteria dextran on the properties of faba bean protein food, the textural and rheological properties etc. of the faba bean protein composite gels induced by GDL with different concentrations of dextran were determined by using Haake rheometer and texture analyzer. The results showed that dextran significantly increased the water holding of faba bean protein gel. The water holding index of faba bean protein gel was 60.38%, and the index of the composite gel with 1% dextran was 70.08% (p<0.05). The concentration of dextran between 0.25% and 1% had a certain effect on the color of composite gel, the color of faba protein gel could be adjusted. The strength of the composite gel with 0.5% dextran was 0.27 N, which was significantly different from that of faba bean protein gel (0.37 N). The texture properties of foods containing high faba protein were softened by the addition of dextran. The flow curve of the dextran /faba bean protein composite gel conformed to the Carreau model (R2>0.99), showing the properties of pseudoplastic fluid. The elastic modulus G' of strain scanning was higher than the viscous modulus G", indicating that the elasticity of the dextran/faba bean protein composite gel was dominant. The results of frequency scanning showed that dextran could soften the gel and significantly reduced the elastic modulus and viscous modulus of faba protein gels, thereby being more suitable for processing. The texture and rheological properties of faba protein were improved by adding dextran to faba food, which provided reference for expanding its application range.
    10  Preparation and Physicochemical Properties of Hollow Salt Microspheres
    ZHANG Yang LI Yanlei LUO Wei ZHAO Ruxia WANG Jinmei YANG Xiaoquan
    2022, 38(4):76-82. DOI: 10.13982/j.mfst.1673-9078.2022.4.0735
    [Abstract](191) [HTML](173) [PDF 1.28 M](564)
    Abstract:
    In this study, spray-dried hollow salt microspheres were prepared by using gum Arabic (GA) as the main additive and the effects of processing conditions on the physicochemical properties of the hollow salt microspheres were investigated. The results showed that GA migrated quickly to the surface of the hollow salt microspheres due to its excellent surface activity and the atomic percentage of C and O on the surface of the microspheres was increased to 41.03% and 10.97%, respectively. Hollow salt microspheres with minor size (10~20 μm), low volume density (0.45 g/mL) and high specific surface area were produced. The hollow structure was in favor of the rapid melting of salt particles (60 s), which was 2.67-fold faster than normal table salt. The particle size of salt microspheres was highly related to the GA concentration. Thicker GA shell was formed and the particle size was subsequently raised from 12.49 μm to 19.23 μm with the concentration of GA increased from 0.50% to 0.75%, while minor-sized microspheres were fabricated at high concentration (1.0%) due to the effect of GA on reducing the formation and growing time of crystal nucleuses. The salt concentration had no significant effect on the particle size of hollow salt microspheres. Decreasing inlet temperature facilitated the formation of hollow salt microspheres with small particle size (15.30 μm). GA concentration of 0.5%, salt concentration of 35%, and inlet temperature of 130 ℃ were considered to be the best processing conditions. The minor size and hollow structure of hollow salt microspheres improved the salt dispersity on the surface of fried peanuts, decreased the granular sensation and enhanced the salinity and characteristic flavor of fried peanuts compared to equal amount of normal table salt and salt-GA mixture. This study could provide the theoretical guidance for the development of salt-reduced food.
    11  Reactive Oxygen Metabolism Balance in Actinidia arguta by 1-MCP Treatments
    CHEN Xiran ZHANG Peng JIA Xiaoyu LI Jiangkuo
    2022, 38(4):83-91. DOI: 10.13982/j.mfst.1673-9078.2022.4.0811
    [Abstract](233) [HTML](233) [PDF 927.83 K](497)
    Abstract:
    To analyze the effects of 1-methylcyclopropene (1-MCP) treatments on the reactive oxygen species (ROS) metabolism in Actinidia arguta stored at -0.5±0.3 ℃ for 0~60 d, the changes in the fruit physiology, ROS metabolism, and ascorbate-glutathione cycle of Actinidia arguta “Longcheng NO.2” treated with 1-MCP at 1.0 μL/L were examined. The results suggest that, at 60 d, the respiration intensity and ethylene production rate of the 1-MCP-treated fruits are 44.11 mg/(kg•h) and 2.35 μL/(kg•h), respectively. These values are 17.20% and 23.70% lower than those of the control group (CK), correspondingly. In addition, owing to the 1-MCP treatment, the increases in the levels of superoxide anion, hydrogen peroxide, and malondialdehyde and the relative conductivity were alleviated, whereas the activities of superoxide dismutase, catalase, peroxidase, ascorbate peroxidase, and glutathione reductase were well maintained. Simultaneously, the reduced ascorbic acid, oxidized glutathione, and reduced glutathione levels were maintained at relatively high levels. In summary, 1-MCP treatments can effectively reduce the respiration intensity and ethylene production rate of Actinidia arguta as well as the production of free radicals. In this manner, the antioxidant capacity during storage is enhanced and the aging of the fruits is slowed down, maintaining the commercial value of the fruits. Hence, the findings of this study can help theoretically and technically to improve the postharvest storage and preservation technology of Actinidia arguta.
    12  Effect of Soaking Treatment of Wheat Starch before Gelatinization on Liangpi Quality
    ZHANG Bobo ZHENG Shuaishuai AI Zhilu FAN Chaoqi CHENG Zenghui JIAO Honggang WANG Chao FAN Huiping
    2022, 38(4):92-98. DOI: 10.13982/j.mfst.1673-9078.2022.4.0803
    [Abstract](357) [HTML](261) [PDF 1.25 M](622)
    Abstract:
    Owing to the poor adhesion of pure wheat starch (WS), Liangpi is traditionally made from wheat flour residues without gluten, and its production has not been standardized or industrialized. To provide theoretical support for the industrialization of Liangpi production, the effects of soaking WS in water (for 0–24 h) before gelatinization on the quality of Liangpi were examined. The sensory score and quality of Liangpi were remarkably increased after soaking WS for an extended duration. After soaking WS for 24 h, the elasticity, storage modulus, short-range molecular order structure level, and relative crystallinity of Liangpi increased significantly by 213.64%, 122.01%, 6.86%, and 41.68%, respectively, compared with the values in the control group (without soaking). These results suggest that soaking before gelatinization promotes the formation of WS gel. According to SEM images, the pore size of Liangpi decreases with an increased soaking duration. In addition, the content of amylose in WS granules decreases by approximately 16.58% after 24 h of soaking compared with that in the control group. However, the elasticity of Liangpi was reduced by 40.38% for WS alone when the soaking water was discarded. These results reveal that Liangpi formation is closely related to the amylase content in water after soaking. Therefore, the quality of Liangpi can be improved by soaking before gelatinization of WS to enhance amylose exudation. This study provides reference data for the processing of Liangpi.
    13  Structure and Property of Waxy Starch-chitosan Crosslinking Films Regulated by Vanillin
    ZHANG Xuxin ZHANG Shuyan LI Lin ZHU Jie ZHAO Lei
    2022, 38(4):99-105. DOI: 10.13982/j.mfst.1673-9078.2022.4.0635
    [Abstract](207) [HTML](143) [PDF 66.82 M](955)
    Abstract:
    Waxy cornstarch-chitosan crosslinking films were prepared with vanillin. Hydrophilicity, morphology, structures and thermal properties of crosslinking films were studied with vanillin addition. When crosslinking films were prepared without vanillin, watercontact angle of samples was decreased from 82.88°(7:3) to 75.37°(0:10), the unevenness of cross-sections was decreases and then increased, the wave number of -N-H deformation vibration was reduced, and the decomposition temperature of starch/chitosan was declined from 336.23 ℃ (10:0) to 310.83 ℃ (0:10). Comparatively, when the films were regulated by vanillin, water contact angles and roughness were both increased with the increased mass ratio of chitosan, the wave number of -N-H deformation vibration and the decomposition temperature of starch/chitosan was declined. Results indicated that three kinds of interactions (vanillin-chitosan, waxy starch-chitosan, waxy starch-vanillin) were formed in the films, the formation of -C=N bond between -CHO of vanillin and -NH3+ of chitosan weakened the hydrogen-bond interactions between chitosan and starch, which helped to regulate the structure and property of starch-chitosan films. The investigation provided data support and theoretical basis for broadening the application of starch and chitosan in active food packaging.
    14  Optimization of Fermentation Technology of Citrus Black Tea Beverage
    XU Jiahao LIN Jingchang ZHANG Shuning DONG Siyuan ZHONG Xianfeng CHEN Wensheng CHEN Lipei
    2022, 38(4):106-113. DOI: 10.13982/j.mfst.1673-9078.2022.4.0724
    [Abstract](245) [HTML](195) [PDF 979.33 K](641)
    Abstract:
    In order to determine the best fermentation parameters of citrus tea fungus beverage, in this experiment, Xinhui citrus and Yingde black tea were used as main raw materials to produce citrus black tea beverage by inoculation of black tea fungus. Based on the single factor experiment, Box-Behnken response surface method was used to design the experiment, using four factors and three levels, taking pH value as the response index, to explore the influence of four factors on the quality of citrus black tea beverage, such as the amount of lactic acid bacteria powder, the amount of sucrose, the fermentation time and the fermentation temperature, and to determine the best technological parameters of citrus black tea beverage fermentation. The results showed that the optimum fermentation conditions of citrus tea fungus beverage were as follows: lactic acid bacteria 0.4%, sucrose 15%, fermentation temperature 32 ℃ and fermentation time 4 days. Under these conditions, the pH value is 3.14, the sensory evaluation score is 93.1 points, and the consumption of soluble solids is 12.30%, which is close to the results of three verification tests, indicating that the regression model fits well. Therefore, the response surface method to optimize the fermentation process of citrus black tea beverage is reliable and can provide a scientific basis for the research and development of citrus black tea beverage.
    15  Effect of Alkaline pH on the Thermal Aggregation Behavior of Mackerel Myosin
    HA Si HAN Lingyu XU Zhe MA Kun LI Tingting
    2022, 38(4):114-120. DOI: 10.13982/j.mfst.1673-9078.2022.4.0719
    [Abstract](154) [HTML](397) [PDF 751.13 K](458)
    Abstract:
    In order to explore the effect of alkaline pH on the thermal aggregation behavior of mackerel myosin, mackerel myosin was used to explore the effect of pH (7.0, 8.0, 9.0) on the structure and structure of myosin under heating conditions. For the influence of physical and chemical properties (solubility, turbidity, secondary structure, total sulfhydryl content, surface hydrophobicity), the unheated group was used as a blank control group. The results showed that the solubility of myosin in the control group increased from 68.00% to 82.00% at pH (7.0, 8.0, 9.0), and the turbidity did not change significantly; there was a big difference in the heating group, and the solubility increased from 30.00% to 94.00% , The turbidity absorbance value decreased from 0.49 to 0.23; the content of myosin α-helix at pH 9.0 in the heating group decreased, and the content was the lowest among all groups, at 45.60%, and the β-sheet content increased at 10.60%; the sulfhydryl group content of the heating group showed a downward trend, from 70.45 nmol/mg to 50.11 nmol/mg. The protein at alkaline pH facilitates the conversion of sulfhydryl groups into intermolecular and intramolecular disulfide bonds; as the pH value increases, for the myospheroids of the control group, the surface hydrophobicity coefficient of the protein increased successively, while the heating group decreased, but the surface hydrophobicity coefficient of myosin in the heating group was still much higher than that of the control group. In summary, by exploring the properties of myosin thermal aggregates under alkaline conditions, it will conducive to regulate their thermal aggregation and obtain a myosin solution with better thermal stability. The addition of emulsifiers to food is of great significance.
    16  Effects of Ultrasonic Treatments on the Quality of Kazakh Lamb
    ZHANG Li SUN Jianing ZHU Mingrui XING Shijun LIU Guangjuan XIAO Zhiyuan JIANG Meiling WANG Zirong
    2022, 38(4):121-128. DOI: 10.13982/j.mfst.1673-9078.2022.4.0808
    [Abstract](163) [HTML](304) [PDF 445.42 K](513)
    Abstract:
    To examine the effects of ultrasound on lamb quality, ultrasonic treatments (at 40 kHz and 100 W for 0, 20, and 40 min) were performed on the semimembranosus muscles of sheep. The physical and chemical properties as well as the myofibrillar protein characteristics of the lamb were measured at 4 ℃ at 0, 2, 12, 24, 72, 120, and 168 h. The results demonstrate that during aging, the quality of lamb meat after the ultrasonic treatment is significantly better than that of the untreated lamb meat which was directly subjected to cold storage (p<0.05). After 168 h, the pH value (5.58), L* value, myofibrillar fragmentation index (69.40), and protein solubility (17.40%) of the lamb meat subjected to 20-min ultrasonic treatment were considerably higher than those of the untreated lamb meat subjected to direct cold storage (p<0.05), whereas the shear force of the former (37.54 N) was remarkably lower than that of the latter (p<0.05). These indicators reveal the improved quality of the ultrasonic-treated lamb meat. In addition, the cooking loss of the semimembranosus muscles from the lamb meat that underwent 40-min treatment (26.23%) was greater than that from lamb meat treated for only 20 min (25.83%). Hence, to reduce the quality loss and energy consumption, ultrasonic treatments for approximately 20 min are recommended. The aging time of semimembranosus muscles from the ultrasonic-treated group was 24 h, which was 48 h shorter than that of muscle from untreated lamb (72 h). Overall, ultrasonic treatments can enhance lamb tenderness and improve meat quality while shortening the aging time.
    17  Establishment and Validation of Prediction Model for the Secondary Precipitate in High-salt Diluted-state Soy Sauce
    FENG Tuo SHAN Pei LIN Hong XU Ting WANG Bo ZHANG Zhihong GAO Xianli
    2022, 38(4):129-139. DOI: 10.13982/j.mfst.1673-9078.2022.4.0782
    [Abstract](165) [HTML](271) [PDF 694.23 K](534)
    Abstract:
    In order to establish the prediction model of secondary precipitate of high-salt dilute-state soy sauce, the physicochemical indexes related to the formation of secondary precipitate of soy sauce were comparatively analyzed, including pH value, iron ion, ferrous ion, polyphenol, polysaccharide, sodium chloride, ethanol, glutamic acid and temperature. SPSS software was used to analyze the correlation between the amount of secondary precipitate and each index, a multiple linear regression prediction model based on the correlation analyses was established. The results showed that the amount of secondary precipitate (Y, g/L) was significantly correlated with pH value (X1) (p<0.01), iron ion (X2, mg/L), polyphenol (X4, g/100 mL) and sodium chloride contents (X6, g/100 mL) (p<0.05), and the multiple linear regression equation amongst them was successfully established. The results showed that there was a good correlation between the predicted value of multiple linear regression equation and the measured value after 3 months storage (R2=0.8517). The prediction model is helpful to find the soy sauce that will produce serious secondary precipitate in advance, and avoid its inflow into the market and cause economic and reputation losses to enterprises, thus it has important application value.
    18  Improving Texture Properties of Sliced Potatoes during Thermal Processing by Acetic Acid in Synergy with Calcium Chloride
    ZOU Yan LI Jiarong LI Fei YU Xiaoyan YU Shirui LEI Zunguo DONG Nan
    2022, 38(4):140-146. DOI: 10.13982/j.mfst.1673-9078.2022.4.0580
    [Abstract](171) [HTML](211) [PDF 1.43 M](542)
    Abstract:
    In order to solve the problem regarding the loss of crisp texture of potato chips after full thermal processing, the effects of acetic acid and calcium and their synergistic pretreatment on the texture of potato slices after thermal processing were studied in this experiment. Sliced potatoes (SP) pretreated with acetic acid and calcium chloride was considered as a strategy to enhance the textural characteristic of SP after thermal processing. Base on the single factor test, the variation of hardness of SP after thermal processing was used as the response value. In order to acquire optimal formula of pretreatment for SP, the response surface design was used. On the basis of the single factor experiments, taking the acetic acid concentration, calcium chloride concentration and soaking time as the design factors, and the hardness of potato slices after cooking for 10 min as the response value, the response surface method was used to optimize the synergistic action of acetic acid with calcium chloride to improve the hardness of potato slices. Finally, the optimal parameters for improving the hardness of potato slices were determined as follows: acetic acid 5.26 mL/L; calcium chloride 5.28 mg/L; soaking time 11.1 h. In the meantime, the microscopic examinations and X-ray microanalyses of different samples revealed that the cells of the heat-treated potato slices had severe intercellular separation, whilst the cells of potato slices treated with acetic acid in synergy with calcium chloride followed by steaming for 10 min were still tightly connected. The amount of the permeated calcium in the potato slices soaked with both acetic acid and calcium chloride was significantly higher than that for the slices soaked with calcium chloride only. The results further revealed the reason behind the improved hardness of potato slices pretreated with acetic acid and calcium chloride, which also provides a basis for the late-stage processing of flavored instant potato chips.
    19  Regulation of Mulberry Polyphenols and Their Microcapsules on the Structure and Gel Properties of Myofibrillar Proteins under Oxidative Stress
    HOU Yuxue LI Denglong LIN Weiling LIU Xueming LIN Yaosheng TANG Daobang WANG Xuping ZHANG Xianbin YE Yuyou CHENG Jingrong ZHU Mingjun
    2022, 38(4):147-154. DOI: 10.13982/j.mfst.1673-9078.2022.4.0566
    [Abstract](182) [HTML](349) [PDF 1.24 M](553)
    Abstract:
    The effects of mulberry polyphenols (MPs) and mulberry polyphenols-β-cyclodextrin (CD) microcapsules (MPMs) on the structure and functional properties of pork myofibrillar proteins in a LOX oxidation system were investigated. MPs, β-CD and MPMs were respectively added to myofibrillar proteins, and oxidation in the LOX oxidation system. Then, the effects of MPs and their microcapsules on the structure and gel properties of myofibrillar proteins were analyzed. MPs could effectively inhibit the carbonylation of myofibrillar protein, reduce the conversion of sulfhydryl groups to disulfide bonds, and improve the water-holding capacity of protein gel. Compared with the oxidation group, after the treatment with MPs, the carbonyl content decreased by 33.33%, the sulphydryl content increased by 54.87%, and the disulfide bond content decreased by 19.38%, which increased the water-holding capacity of the proteins from 20.22% to 23.11% (p<0.05), but the thermal stability of myofibrillar proteins modified with MPs. The encapsulation of MPs by β-CD decreased the negative effect of MPs on the whiteness of gel, and improved the structure and water-holding capacity of the gel through strengthening the hydrogen bonds between proteins. These above results showed that the MPMs made with β-CD as the wall material helped improve the oxidative stability and gel structure of myofibrillar proteins, and are expected to be used as a protein oxidation regulator for meat processing.
    20  Effect of High Hydrostatic Pressure Treatment on Inactivation of Oxidase in Non-concentrated Bayberry Juice
    WU Xinyi MENG Ziyi ZHU Yinfei LUO Ziyang WANG Chao DUAN Hanying
    2022, 38(4):155-160. DOI: 10.13982/j.mfst.1673-9078.2022.4.0748
    [Abstract](249) [HTML](202) [PDF 513.34 K](641)
    Abstract:
    The effect of different high hydrostatic pressure (HHP) treatments (300~600 MPa/0~30 min) on polyphenol oxidase (PPO) and peroxidase (POD) in non-concentrated (NC) bayberry juice was studied. Compared to the traditional heat inactivation, the relationship between HHP treatment and enzyme activity was established by fitting the first-order kinetic regression equation and relevant parameters (pressure pulse effect PE, pressure pulse number value ND, equivalent damage value Dp and inactivation rate K of enzyme) were obtained. The results showed that higher pressure (400~600 MPa) had inactivation effects on PPO and POD, in which 600 MPa/10 min could inactivate 90% of PPO activity and 600 MPa/20 min could inactivate 80% of POD activity. Repeated cycle at 600 MPa/30 min did not increase the passivation effects. The linear regression equation (R2>0.8) was obtained by fitting PPO and PPO activity with pressure. With the increase of pressure from 300 MPa to 600 MPa, the K value of PPO increased from 3.03×10-2 to 12.12×10-2, and the K value of POD increased from 1.23×10-3 to 7.67×10-3. At 600 MPa, the ND of PPO and POD were 1.04 and 1.59, respectively, and the Dp values were 19. At the same time, the effects of pressure, holding time and their interaction on PPO and POD activity were extremely significant (p<0.001). Therefore, HHP had a good inactivation effect on the key oxidase in bayberry juice, which could provide a scientific basis for the application of HHP in NC bayberry juice processing.
    21  Comparison of Gel Properties of Tofu Prepared with Different Amounts of Physalis
    ZHANG Zhiheng CHEN Zhenjia LI Yu'e WANG Xiaowen ZHANG Xuebing
    2022, 38(4):161-170. DOI: 10.13982/j.mfst.1673-9078.2022.4.0924
    [Abstract](255) [HTML](327) [PDF 1.46 M](761)
    Abstract:
    After preparing soybean milk from soybeans of the same variety, different proportions of physalis (20%, 23%, and 26%) were added. The influence of physalis addition on the gel characteristics of the resulting tofu was analyzed by determining the texture, chemical force, SDS-PAGE pattern, microstructure, infrared spectrum, and other metrics. The texture of tofu was enhanced with increasing amounts of added physalis; the hardness of tofu reached a maximum value of 3.53 N when 26% of physalis was added. Under these conditions, the water content, water retention, and yield of tofu decreased significantly (p<0.05), whereas the protein content increased from 13.09% to 15.28%. Disulfide bonds and hydrophobic interactions were the main forces driving tofu formation containing physalis, accounting for more than 90% of the total forces, whereas the contribution of ionic bonds and hydrogen bonds was relatively low. Hydrophobicity in 7S proteins was the main force driving gel formation. Some A subunits of 11S proteins were connected by ionic bonds and hydrogen bonds to form the gel. In contrast, macromolecular protein aggregates as well as B and A3 subunits of 11S proteins mainly participated in gel formation through disulfide bonds. As the amount of added physalis increased, the gel network structure of the resulting tofu gradually became rough, and the α helix content decreased from 16.00% to 15.83%. Random coils decreased from 16.04% to 15.80%, and β turns decreased from 34.12% to 33.57%, whereas β folds increased from 33.84% to 34.80%. In summary, the amount of added physalis significantly affects the gel properties of tofu.
    22  Optimization of High Quality Noodles Made by Raw Purple Sweet Potato Flour
    XIONG Tian HE Jianjun CAI Fang WANG Shaohua SHI Jianbin CAI Sha SUI Yong CHEN Xueling FAN Chuanhui JIA Zhiwen MEI Xin
    2022, 38(4):171-181. DOI: 10.13982/j.mfst.1673-9078.2022.4.0630
    [Abstract](180) [HTML](309) [PDF 987.48 K](517)
    Abstract:
    In order to solve the problems of high breaking rate, easy paste soup, poor palatability and dim color in the production of purple potato raw whole powder noodles, the formula and technology of purple potato raw whole powder noodles were optimized with the goal of improving color and texture quality. The influences of color-retaining agents such as citric acid, sodium phytate, kojic acid, tea polyphenols and β-dextrin, and improvers such as carrageenan, gluten and konjac glucomannan at different addition levels on the color, texture and sensory score of the noodles. With the aid of other instruments and equipment including the mixolab2 mixing device, the silty characteristics and gelatinization properties of the mixed powder with raw purple sweet potato flour at different addition levels of were compared and analyzed. The results showed that the raw purple sweet potato flour affected the weakening of protein network, starch gelatinization and amylase-catalyzed starch hydrolysis, with the addition greater than 10% decreasing the gluten and kneading resistance of dough. The suitable color-retaining agent and suitable modifier were 0.6% citric acid and 0.6% KGM, respectively, with their sensory scores increasing by 13.56% and 11.92%, respectively, compared with their corresponding reference groups. On this basis, the optimal formulation process of high-quality raw whole purple potato powder noodles was determined through four-factor and three-level orthogonal experiments, and the optimal conditions were: addition of raw whole purple potato powder 8%, water 32%, citric acid 0.6%, and KGM 0.6%, with the corresponding sensory score being 85.48. The research lays the foundation for subsequent industrial production of high-quality noodles made with raw whole purple sweet potato flour.
    23  Effects of Different Treatment Methods on Solubility of Extruded Corn Flour
    ZENG Deyu LI Jing CHEN Junhong WANG Yang YE Yang ZENG Zhen
    2022, 38(4):182-190. DOI: 10.13982/j.mfst.1673-9078.2022.4.0819
    [Abstract](145) [HTML](277) [PDF 429.26 K](495)
    Abstract:
    The effects of phosphorylation, enzymatic treatments, dry heat treatments, and combined treatments on the water absorption index, water solubility index, dispersion time, agglomeration rate, viscosity, particle size, and chrominance of extruded corn flour were analyzed to improve the quality of extruded corn flour. Phosphorylation heat treatments did not significantly affect the viscosity of extruded flour, whereas dry heat treatments by edible gum increased the viscosity. Additionally, enzymatic treatments alone and in combination with phosphorylation and dry heat treatments significantly reduced the water absorption index and viscosity. Specifically, the water solubility index of the flour after enzymatic treatments and combined treatments was greater than that after phosphorylation and dry heat treatments. The water solubility index of the extruded corn flour treated with α-amylase increased from 30.4% to 80.98%, whereas the water absorption index decreased from 4.96 to 1.21. The agglomeration rates of extruded corn flour treated with xanthan gum combined with α-amylase and α-amylase combined with sodium orthophosphate were 1.63% and 1.46%, respectively. These values are lower than those after treatments by sodium orthophosphate, α-amylase, and neutral protease individually, and dry heat treatment by xanthan gum (5.57%, 5.60%, 6.16%, and 5.58%, respectively). In addition, the viscosity of the two products after the combined treatment methods were 83.33 and 100 mPa•s, suggesting that the fluid was relatively thin. Samples subjected to dry heat treatments with sodium orthophosphate and xanthan gum showed viscosities of 2243.33 and 306.33 mPa•s, respectively. The viscosity and agglomeration rate of extruded corn flour treated with sodium orthophosphate combined with neutral protease were 546.67 mPa•s and 4.89%, respectively. The viscosity was moderate, and the agglomeration rate was lower than those after single treatments. For this extruded flour, the dispersion time and particle size were 4.81 s and 131.14 μm, respectively, and the resulting fluid was light yellow. Extruded corn flour is more soluble after combined treatment with sodium orthophosphate and neutral protease
    24  Process Optimization of Microwave-vacuum Freezing Combined Drying of Green Pepper by Response Surface Methodology and Its Quality Analysis
    XIAO Manyu ZHANG Xiuling LIU Minghua LI Chen
    2022, 38(4):191-200. DOI: 10.13982/j.mfst.1673-9078.2022.4.0513
    [Abstract](239) [HTML](178) [PDF 2.55 M](568)
    Abstract:
    In this study, green pepper was dried by the microwave-vacuum freezing combined drying method. The effects of microwave power, moisture content at the intermediate conversion point and vacuum freeze-drying time on the vitamin C content, sensory score, rehydration ratio and a* of the dried green pepper were studied. The microwave-vacuum freezing combined drying process was optimized by the single factor experiments and response surface experiments, and the nutritional components and flavor of the green pepper products via combined drying were compared and analyzed. Finally, the effects of microwave-vacuum freezing combined drying, microwave drying and vacuum freeze drying on the drying quality of green pepper were studied. The results showed that the optimal process conditions for combined drying were microwave power 381.17 W, moisture content at the intermediate conversion point 61.81%, vacuum freeze drying time 12.04 h. The three kinds of combined drying green pepper products showed certain differences in nutritional contents and flavors. The green pepper products subjected to microwave-vacuum freezing combined drying and vacuum freeze drying were superior to those subjected to microwave drying in terms of texture, microstructure, and certain physicochemical indexes.
    25  Difference Analysis of Volatile Components in Cinnamon from Different Producing Areas
    WANG Zeliang ZHANG Min QIU Daofu LI Jiezhi LI Long WAN Minhui FAN Zhiyi LYU Pengjun DENG Weiqin LI Heng
    2022, 38(4):201-207. DOI: 10.13982/j.mfst.1673-9078.2022.4.0747
    [Abstract](249) [HTML](293) [PDF 1.32 M](908)
    Abstract:
    To explore the differences of volatile components in cinnamon from different producing areas and provide reference data for the selection of raw materials for bittern production, HS-SPME and GC-MS were used to determine volatile components in cinnamon samples from Chengdu, Guangxi, Hanyuan and Yunnan. PCA and PLS-DA analysis were performed combined with OAV values. The results showed that 66 components were detected in cinnamon samples from different producing areas, including 43 terpenoids, 11 aldehydes and ketones, 2 alkanes, 4 alcohols, 2 esters and 1 indole. The volatile compounds in cinnamon samples were mainly terpenoids, and their relative contents ranged from 2706.76 μg/kg to 34337.44 μg/kg. γ-terpinene (OAV: 1132.03~5721.45), α-carvene (OAV: 16.66~264.45), (E)-cinnamaldehyde (OAV: 18.04~103.07) and p-cymene (OAV: 31.00~43.39) had relatively high OAV values, which contributed significantly to the main flavor formation of cinnamon.PCA and PLS-DA analysis showed that there were significant differences in volatile flavor of cinnamon samples from different regions. No characteristic aroma was found in cinnamon from Chengdu, but the main aroma was strong. The typical cinnamon aroma of Guangxi cinnamon, such as spicy flavor and flower and wood fragrance, was the most obvious. The sweet and fragrant aroma of Hanyuan cinnamon is relatively prominent; Yunnan cinnamon has a strong fruit aroma. The place of origin has great influence on the volatile flavor of cinnamon samples.
    26  Effect of Fermentation Time on the Aroma Components of Whole-Grain Highland Barley Flour Slurry
    ZHU Junchao ZHANG Chengzhi ZHANG Zengjiang ZHENG Bo CHEN Ling
    2022, 38(4):208-215. DOI: 10.13982/j.mfst.1673-9078.2022.4.0579
    [Abstract](170) [HTML](166) [PDF 1.26 M](662)
    Abstract:
    In this study, the volatile components in the whole-grain highland barley slurry were analyzed by headspace-solid phase microextraction-gas chromatography-mass spectrometry (GC-MS), and the volatile compositions of the whole grain slurry after fermentation for different times as well as their contributions to the aroma and flavor performance of the flour slurry were also examined. The results showed that 14 volatile components were detected in unfermented flour slurry, with 6 compounds being key components and n-hexanal being the most significant contributor. Twenty-two volatile components were detected in the slurry after fermentation for 3 h, with nonanal being the only key volatile component. Twenty-two volatile components were detected in the slurry after fermentation for 6 h, with 5 compounds being the key components and ethyl octanoate being the most significant contributor. Twenty-four volatile components were detected in the slurry after fermentation for 12 h, with nonanal and (E, E)-2,4-nonadienal being the key volatile components. Compared with the fermentation for other time periods, the fermentation for 6 h led to the highest relative content of esters (50.20%) in the slurry, which contributed to a pleasant fruity aroma. The key volatile components in the slurry after fermentation for 6 h were ethyl caproate (14.94%) and ethyl caprylate (17.87%), with their ROAV values being 19.45 and 100, respectively. The research can provide basic data for the creation of whole-grain highland barley-based fermented foods.
    27  Neurotoxicity Prediction Model of Food Contaminants Based on Machine Learning
    ZHOU Yue LI Xiaolan CHENG Wei FENG Yan WANG Yan
    2022, 38(4):216-223. DOI: 10.13982/j.mfst.1673-9078.2022.4.0727
    [Abstract](231) [HTML](193) [PDF 714.05 K](629)
    Abstract:
    Prediction models based on machine learning algorithms are established to predict the neurotoxicity of chemical pollutants in food. Database which includes fifty-seven neurotoxic compounds and fifty non-neurotoxic compounds was established through the published paper. By utilizing the R and SPSS software, the random forest (Random Forests, RF), neural network (Artificial Neural Network, ANN), support vector machine (Support Vector Machine, SVM) and other algorithms were used to build the classification models applying the molecular descriptors. The random forest algorithm represented the best performance in aspects of total accuracy and feasibility, illustrating total accuracy of training set and test set was 95.51% and 83.33%, respectively. The area under the curves of training set and test set were 0.99 and 0.85, respectively. The accuracy of 10-fold cross-validation was 70.24%. In this study, the prediction models established on the basis of machine learning algorithms and chemical informatics can accurately distinguish the neurotoxicity compounds from non-neurotoxicity compounds. Our result suggested that among models, the one constructed with the random forest algorithm performs better and the highest eigenvalue from Burden matrix as a molecular descriptor contributes dominantly to the classification of chemicals with neurotoxic potential.
    28  Comparison of Quality and Toxicity of Polycyclic Aromatic Hydrocarbons in Six Kinds of Liquid Smoke
    WANG Fei QIAO Mingwu HUANG Xianqing SONG Lianjun XUE Guizhong MENG Shaohua ZHAO Jiansheng MA Xiangjie
    2022, 38(4):224-233. DOI: 10.13982/j.mfst.1673-9078.2022.4.0799
    [Abstract](177) [HTML](184) [PDF 671.44 K](557)
    Abstract:
    In this paper, the quality and polycyclic aromatic hydrocarbons (PAHs) content of garlic stick, Perilla frutescens, dried tangerine peel, pear wood, peach wood and jujube wood liquid smoke were studied, and toxic equivalent factors (TEFs) was used to evaluate the toxicity risk of liquid smoke. The results showed that there was the significant difference in quality index of liquid smoking, the density was 1.01~1.04 g/mL, the color L* value was 45.01~67.56, the a* value was 23.06~29.86, the b* value was 86.68~96.16, the soluble solid content was 8.90%~15.77%, the pH value was 2.54~8.65, the total phenol content was 1.95~8.18 mg/mL, the carbonyl compound content was 2.23~19.81 g/100 mL, and the total acid content was 1.53%~12.51%. There was a great correlation among the indexes of liquid smoke. In the positive correlation, the density had the strongest correlation with the content of soluble solids, and the correlation coefficient was 0.998; in the negative correlation, color b* value had the strongest correlation with soluble solid content, and the correlation coefficient was -0.986. The 16 kinds of PAHs were detected in the liquid smoke, among which the content of benzo(a)pyrene (BaP) was 0.13~1.53 μg/kg, and the total PAHs content ranged from 123.53 to 2222.31 μg/kg. Acenaphthene was the main PAHs in liquid smoke, and the proportion of 3 rings was the highest, ranging from 60.05% to 97.39%. The toxic equivalent quantity (TEQ) values of 16 kinds PAHs in liquid smoke were 5.00 μg/kg (pear wood), 4.25 μg/kg (dried tangerine peel), 3.18 μg/kg (commercial), 2.25 μg/kg (Perilla frutescens), 1.74 μg/kg (garlic stick), 1.37 μg/kg (peach wood) and 0.64 μg/kg (jujube wood). Through the determination of related indexes, as well as the analysis on distribution characteristics of PAHs and toxicity risks in liquid smoke, it can provide reference and basis for the safe use of liquid smoke.
    29  Determination of Five Skin Ingredients in Asini Corii Colla Foods Based on Marker Peptide
    ZHAO Yanxia GONG Liping SHI Feng HANG Baojian LUO Xuegang
    2022, 38(4):234-240. DOI: 10.13982/j.mfst.1673-9078.2022.4.0676
    [Abstract](199) [HTML](223) [PDF 701.58 K](607)
    Abstract:
    To establish the method based on marker peptide for determination of the skin ingredients of horse, ox, pig, camel and deer in asini corii colla foods by HPLC MS/MS. The extraction methods and enzymolysis conditions of the samples were investigated, and the separation effects of different chromatographic conditions on the components of various skin sources were compared. The mobile phase elution system was optimized. The samples were hydrolyzed by trypsin at 37 ℃. The separation was performed with the gradient elution using the mobile phase consisting of 0.1% formic acid water solution and 0.1% formic acid acetonitrile solution at a flow rate of 0.3 mL/min. The column temperature was set at 40 ℃ and a sample size of 2 µL. Marker peptides were determined in the modes of electrospray positive ionization (ESI+) and multiple reaction monitoring (MRM) with matrix matching scaling quantification. Horse and deer marker peptide showed good linear relationships within the range of 5~500 ng/mL, others showed good linear relationships within the range of 10~500 ng/mL. The limit of detection was 0.082~1.1 mg/kg. The spike recoveries (n=6) of dimethyl sulfate were 86.8%~103.5%. Marker peptides of horse and ox were detected in 29 samples. The method is specific, sensitive, simple, rapid and accurate, and can be used for the the determination ofthe skin ingredients of horse, ox, pig, camel and deer in asini corii colla pastry and asini corii colla drinks.
    30  Determination of Morpholine Fatty Acid Salt Residues in Fruits by Improved QuEChERS-Gas Chromatography- Tandem Mass Spectrometry
    CHEN Li ZHU Zhengwei WANG Wei RONG Mao JIANG Feng WANG Huixia
    2022, 38(4):241-247. DOI: 10.13982/j.mfst.1673-9078.2022.4.0606
    [Abstract](184) [HTML](166) [PDF 1021.17 K](437)
    Abstract:
    In this study, a gas chromatography-tandem mass (GC-MS/MS) method was developed to determine the morpholine fatty acid salt in fruits. Samples were analyzed using a modified QuEChERS (quick, easy, cheap, effective, rugged and safe) method, through being extracted with 1% formic acid-acetonitrile, purified with ethylenediamine-N-propylsilane (PSA) and graphitized carbon black adsorbent (GCB), derivatized with 5.5 mol/L HCl and 4.3 mol/L NaNO2 in ice bath for 3 h, separated by HP-FFAP capillary column, and measured in electron bombardment ion source (EI) and multiple reaction monitoring mode. Matrix calibration curve and external standard method were used for quantification. The results showed that morpholine fatty acid salts can be derivatized into stable nitrosomorpholine (NMOR) through nitrosation. The peak shape of the target derivative compound was good, The linearity in the analyte range of 1~800 µg/L was good, with the correlation coefficient higher than 0.99, the limit of detection as 0.14~1.09 µg/kg, the limit of quantitation as 0.47~3.63 µg/kg, the recovery rate of spiked standard as 73.6%~118.5%, and the relative standard deviation (RSD) as 3.6%~15.2% (n=6). This method is accurate and efficient, has a high detection rate in actual samples, and is suitable for qualitative and quantitative detection of morpholine fatty acid salt in fruits.
    31  Rapid Discrimination of Base Liquor for Baijiu Based on FT-NIR Spectroscopy and KPCA-MD-SVM
    ZHAI Shuang TUO Xianguo ZHANG Guiyu ZHU Xuemei LUO Lin GAO Jing LUO Qi
    2022, 38(4):248-253. DOI: 10.13982/j.mfst.1673-9078.2022.4.1132
    [Abstract](200) [HTML](171) [PDF 1.86 M](540)
    Abstract:
    To ensure the accuracy of base liquor segmentation in the process of liquor picking, the spectra of base liquor samples during the whole liquor picking process were collected using Fourier transform near-infrared spectroscopy, and support vector machines were used to establish a segmentation model for the spectrum of the base liquor with the optimal pretreatment. The accuracy of the model training set was 93.02%, and the discrimination rate for the test set was 90.08%. To reduce the modeling time and increase the reliability of the model, kernel principal component analysis was used to reduce the dimensionality of the base liquor spectral data. A segmentation model for the base liquor was established. The accuracy of the model for the training set was 94.81%, and the discrimination rate for the test set was 90.75%, which were 1.79% and 0.67% higher than those of the model without kernel principal component analysis, respectively. To further improve the discrimination ability of the model, the Mahalanobis distance was used to eliminate abnormal data samples after dimensionality reduction. The accuracy of the new model for the training set in terms of the number of base liquor segments was 98.72%, and the accuracy for the test set was 98.75%. The accuracy for the training set and discrimination rate for the test set were improved by 3.91% and 8%, respectively. Thus, the kernel principal component analysis + Mahalanobis distance + support vector machine-based liquor segmentation model can quickly distinguish base liquor, suggesting that near infrared spectroscopy can be applied in automatic liquor picking.
    32  Analysis of the Background Value of Benzoic Acid in Shaanxi Jujube
    LI Xiao LI Yuan ZHANG Jiancheng CHEN Hongjian FAN Cheng LI Mei AN Yu PENG Fei WU Kun LIU Liting
    2022, 38(4):254-259. DOI: 10.13982/j.mfst.1673-9078.2022.4.0616
    [Abstract](196) [HTML](176) [PDF 739.62 K](624)
    Abstract:
    In this study, the benzoic acid contents of 146 batches and 12 varieties of jujube grown in Shaanxi Province were measured, and the effects of development stage, origin, variety and drying method on the endogenous benzoic acid in jujube were investigated. The research results revealed that except for the winter jujube in the green stage, a certain amount of endogenous benzoic acid was found in the other selected jujubes, and its value was usually below 50 mg/kg, rarely exceeded 150 mg/kg. The endogenous benzoic acid in jujube was formed during the different development stages, from green jujube- half-ripe jujube-mature red jujube-dry jujube, and its content increased gradually with the increase of jujube maturity. The variety of jujube had a signigicant effect on the benzoic acid background value of jujube, and the origin had little effect on the benzoic acid background value of jujube. The benzoic acid background value of jujube increased with the increase of drying temperature. The benzoic acid content of the collected jujube samples conformed to the normal distribution, and the maximum confidence interval was 104 mg/kg under 95% probability. This value can be used as the background limit value of benzoic acid in jujube. Within the amount range of normal consumption, the potential impact of the endogenous benzoic acid contained in jujube on human body was much lower than that of benzoic acid at the level defined in the standard for food additive use.
    33  Establishment and Application of Food Traceability System Based on SWOT Analysis
    LI Wanya QU Ge Mary Wong YANG Fangqiong
    2022, 38(4):260-274. DOI: 10.13982/j.mfst.1673-9078.2022.4.0642
    [Abstract](205) [HTML](780) [PDF 897.72 K](522)
    Abstract:
    Food traceability system refers to the ability to track and trace the process covering all links in the food industry chain, which plays an important role in food safety. Although China has promulgated various regulations to strengthen food safety, some gaps in the implementation process may reduce the effectiveness of the law. Based on the current situation a mixed method of data collection was adopted to achieve two objectives: to explore factors affecting the adoption of food traceability systems in China and to construct a food traceability system for a medium-sized restaurant in Hong Kong. In particular, a quantitative method involving a structured questionnaire analyzed the factors affecting food traceability systems, while a case study approach was applied to conduct a thorough SWOT analysis of the proposed food traceability system. The results indicate that consumers’ psychographic characteristics, socio-demographic profiles, and consumer cognition of food safety affect food traceability systems. The SWOT framework revealed that, despite the internal weaknesses and external threats to the proposed system, organizations can take advantage of the strengths and existing opportunities of the system to operate the tool. Literature reference, questionnaire survey and field investigation are used as methods respectively in project, then a traceability system for Wing's Catering was designed and this system was analyzed by SWOT analysis. As a result, the system is simple to operate within low cost, and it is suitable for similar specification like Wing's to apply food traceability.
    34  Isolation and Identification of the Pathogen Associated with Acute Hepatopancreatic Necrosis Disease in Imported Prawns and the Establishment of A Loop-mediated Isothermal Amplification (LAMP) Assay
    HUANG Yanqiong DENG Yan MEN Xingyi CHEN Wanxia BAI Jianshan
    2022, 38(4):275-281. DOI: 10.13982/j.mfst.1673-9078.2022.4.0593
    [Abstract](134) [HTML](181) [PDF 1.51 M](371)
    Abstract:
    In this study, 453 batches of samples were collected from prawns imported from Vietnam, Thailand and Malaysia during 2017~2018, to isolate and identify the pathogens of acute hepatopancreatic necrosis disease (AHPND). The analysis of the obtained results showed that the detection rate of the pathogen isolated from the hepatopancreas tissues of prawns was only 2.87%, whilst the detection rate reached 16.11% after enrichment culture. The prawn samples imported from Vietnam had the highest positive detection rate, with the positive detection rate of 312 batches of samples being 21.79%, and 11 positive strains were isolated with the rate of separation success being 15.07%. In order to identify quickly, sensitively and reliably the AHPND pathogens, a loop-mediated isothermal amplification (LAMP) assay was established for rapid detection of AHPND pathogens in this research. The results showed that only the AHPND pathogen had obvious amplification curve and color change, and the limit of plasmid detection was 1 fg/μL, which was 100 times higher than that of the conventional PCR method, and had high stability. The detection of 37 actual samples showed that the positive rate was 35.13%, which was higher than that of conventional PCR test. The LAMP rapid detection method established in this study is suitable for the specific detection of AHPND pathogens, and the isolation, identification and analysis of pathogens can also provide reference data for laboratory detection of imported prawns.
    35  Research Progress on the Surface Microorganisms of Citri Reticulatae Pericarpium and Derived Microbial Transformation of Flavonoids
    FU Manqin CHEN Yuting WU Jijun YU Yuanshan WEN Jing XU Yujuan
    2022, 38(4):282-291. DOI: 10.13982/j.mfst.1673-9078.2022.4.0526
    [Abstract](497) [HTML](459) [PDF 760.72 K](915)
    Abstract:
    Citri reticulatae pericarpium is a traditional Chinese herbal medicine, which can be used as both medicine and food. It has functions related to the regulation of Qi and spleen strengthening, drying dampness and phlegm reduction. It is rich in flavonoids, volatile oils, alkaloids, limonoids and other trace elements. The flavonoids are the main active components, which exhibit a variety of pharmacological activities, including anti-inflammatory, anti-oxidant, anti-thrombotic, anti-bacterial, anti-viral, hypoglycemic and hypolipidemic effects. It has been widely used in many fields, such as food and healthcare products. However, the applications of flavonoids have been restricted due to their poor solubility and low bioavailability. With the continuous development of biotechnology, microbial transformation of flavonoids and other active substances has become a research hotspot domestically and abroad. It has been reported that the structural characteristics, biological activities of the active components of citri reticulatae pericarpium, as well as its microorganisms and their transformed flavonoids have been deeply and systematically studied. This article reviews the research progress of the microorganisms on the surface of citri reticulatae pericarpium, and their roles in the biotransformation of flavonoids, which provides a reference for the development and application of microbial transformation of active flavonoids from citri reticulatae pericarpium.
    36  Research Progress on Hypoglycemic Effects of Lithocarpus litseifolius
    LI Wenjuan WU Wei WAN Min LI Teng CHEN Sumei YAO Yufei
    2022, 38(4):292-297. DOI: 10.13982/j.mfst.1673-9078.2022.4.0684
    [Abstract](268) [HTML](449) [PDF 523.83 K](1101)
    Abstract:
    Lithocarpus litseifolius, called “sweet tea”, has already been approved as a new food material since 2017. This tea possesses significant activities such as hypoglycemic, hypolipidemic, anti-oxidation, anti-inflammatory and anti-tumor activities. The main active compounds are phlorizin, phloretin, trilobatin and other dihydrochalcones, and its hypoglycemic activities is focused in this review. Persistent hyperglycemia is demonstrated to be common characterization in diabetes which is classified into a chronic metabolic syndrome. Generally, there are some restrictions and side effects on the application of existing hypoglycemic drugs in patients with diabetes. Recently, Lithocarpus litseifolius displays potent research and development value due to its health benefits on hypoglycemic effects with a long history. This article explains the generation of its sweetness in association with its low calories, and summarizes the hypoglycemic activities. Furthermore, the relevant mechanisms of Lithocarpus litseifolius are closely linked to improvement of insulin resistance, glucose transport, regulating enzyme activity, antioxidant defense and diabetic complications. In addition, the present research challenges are mentioned including unclear molecular target and research content limitation. Meanwhile, the related molecular mechanisms referring to hypoglycemic activities of Lithocarpus litseifolius are prospected. Together, this review can provide theoretical basis for Lithocarpus litseifolius to investigate hypoglycemic effects and promote its high value application.
    37  Research Progress of Enzyme-free Electrochemical Sensors for Glucose Based on Transition Metals
    ZHANG Min CAO Jiying WU Shuping WANG Yichen WEN Jiansong QU Zhenghuan ZHANG Dejun
    2022, 38(4):298-306. DOI: 10.13982/j.mfst.1673-9078.2022.4.0618
    [Abstract](263) [HTML](526) [PDF 595.92 K](776)
    Abstract:
    Glucose sensors are widely used in the food industry, and the glucose content is strictly controlled in fermentation production, beverage preparation, and dairy product production. Therefore, it is of great significance to construct a new method for rapid glucose detection. Since transition metal-based sensing materials have the characteristics of rapid response, high sensitivity, good stability, and low cost, they show unique advantages in the field of enzyme-free electrochemical sensors for glucose. Therefore, based on the recent experimental and theoretical studies, this paper provides a systematic review of the recent research progress of transition metal-based enzyme-free electrochemical sensors for glucose. The characteristics of nanostructures of transition metals such as copper (Cu), nickel (Ni), cobalt (Co) and zinc (Zn), and the sensing mechanisms of their oxides for glucose under alkaline conditions, as well as the methods for preparing modified electrodes including electrodeposition, are summarized, in order to provide reference for the construction of high-performance glucose sensor. This review specifically highlights the importance of uncovering the electrical signal sensitivity-enhancing mechanism of transition metal towards glucose, to enable on-demand design and expand the application of transition metal sensors.
    38  Research Progress on Detection Methods of Heavy Metals in Aquatic Products
    NIU Bing ZHONG Hailin ZHAO Chaomin ZHANG Runhe FAN Xiang CAI Wenxuan WU Chiying XU Xiaowei DENG Xiaojun
    2022, 38(4):307-315. DOI: 10.13982/j.mfst.1673-9078.2022.4.0687
    [Abstract](269) [HTML](286) [PDF 435.27 K](651)
    Abstract:
    Heavy metals are concentrated in aquatic products and can enter the body in various ways. When the body's intake of heavy metals exceeds a certain amount, it will cause various hazards to the body. Therefore, more and more scholars pay attention to the fact that the content of heavy metals in aquatic products has gradually become one of the important reasons for limiting the safety of aquatic products, but the content of heavy metals enriched in aquatic products is relatively low, and it is difficult to determine them quickly and accurately. This article mainly reviews the heavy metal detection technology and sample pretreatment methods in aquatic products. It introduces the regulations of various countries and organizations on the heavy metal limit of aquatic products, and focuses on the dry ashing method and sample pretreatment methods commonly used in sample pretreatment methods. Wet digestion, microwave-assisted extraction, ultrasonic-assisted extraction, ion exchange preconcentration, and inductively coupled plasma mass spectrometry, atomic absorption spectrometry, atomic fluorescence spectrometry, liquid chromatography-inductively coupled plasma mass spectrometry in detection technology, as well as the principles, advantages and disadvantages of detection methods, and the detection and development of heavy metals are summarized and prospected.

    Editor in chief:李琳

    Inauguration:现代食品科技

    International standard number:ISSN 1673-9078

    Unified domestic issue:CN 44-1620/TS

    Domestic postal code:46-349

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