Volume 37,Issue 6,2021 Table of Contents

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  • 1  Regulatory Effects of Crude Polysaccharides from Prunella vulgaris L. on Inflammatory Responses Induced by Cadmium in Renal Tubular Epithelial Cells
    CHI Hui-qin LI Zi-yin SONG Jia LAI Yue-fei WANG Yan YANG Jia-ni WAN Yu HE Zhi-ni WEI Qin-zhi WU Wei-liang YANG Xing-fen
    2021, 37(6):1-9. DOI: 10.13982/j.mfst.1673-9078.2021.6.0138
    [Abstract](236) [HTML](317) [PDF 3.07 M](821)
    Abstract:
    The regulatory effects of crude polysaccharides extracted from Prunella vulgaris L. (PVCP) on cadmium-induced inflammatory responses in human renal proximal tubule epithelial cells RPTEC/TERT1 were investigated. Cell counting kit-8 (CCK-8) assay was used to detect the survival rate of RPTEC/TERT1 cells after cadmium or PVCP treatment for 24h. Control group (blank culture medium), model group (8 μmol/L cadmiumchloride) and high-,medium- and low-dose groups of PVCP (8 μmol/L cadmiumchloride +200, 100, 50 μg/mL PVCP)were set in this study. The catalase (CAT) and superoxide dismutase (SOD) activities of RPTEC/TERT1 cells, interleukin (IL)-6, IL-1β, IL-18 and tumor necrosis factor (TNF)-α levels in the supernatant and the protein expression levels of iκB and p65 were evaluated. The results showed that the content of polysaccharides in Prunella vulgaris L. sample was 2.14%. The viability of RPTEC/TERT1 cells was significantly inhibited by cadmium chloride in a dose-dependent manner. In addition, the activities of SOD and CAT of RPTEC/TERT1 cells in the cadmium treated group were significantly decreased (p<0.01)in comparison with control group, while the expression levels of IL-6, IL-1β, IL-18 and TNF-α wereincreased by 9.53 times, 8.80 times, 10.86 times and 1.17 times, respectively (p<0.01). Meanwhile, the expression levels of key proteins iκB and p65 in NF-κB signaling pathway were significantly increased (p<0.05). Compared to the model group, the activities of SOD and CAT were significantly increased (p<0.01), while IL-1β, IL-6, TNF-α and IL-18 as well as iκB and p65 were significantly decreased (p<0.05) after PVCP treatment. In conclusion, PVCP could ameliorate cadmium-induced inflammation in RPTEC/TERT1 cells, and its anti-inflammatory effect may be related to antioxidant response and inhibition of NF-κB signaling pathway.
    2  Protective Effects of Selenium-enriched Bifidobacterium longum DD98 on X-Ray Radiation-induced Hepatic Tissueinjury in Mice
    LU Chun-yi GAO Fei ZHU Hui QIAN Zhi-xiang YANG Ping LI Zhen MAO Wen-wei YIN Yu CHEN Dai-jie
    2021, 37(6):10-19. DOI: 10.13982/j.mfst.1673-9078.2021.6.0957
    [Abstract](340) [HTML](287) [PDF 3.39 M](730)
    Abstract:
    The purpose of this study was to examine the protective effects of selenium-enriched Bifidobacterium longum DD98 (Se-DD98) on X-ray-induced hepatic damage in mice were investigated. Male SPF-grade balb/c mice were randomly divided into 5 groups: normal control group, radiation control group, Se-DD98 high-dose group, Se-DD98 low-dose group and DD98 control group. After 28 days of administration, the body weights of mice were monitored. Whole body X-ray irradiation was used on the 29th day, and then relevant indicators were measured after the experiment. Compared with the body weight (24.44 g) and liver index (4.49%) of the radiation control group, the body weight (26.96 g) and liver index (5.10%) of the Se-DD98 high-dose group increased significantly, with the levels of their serum liver function indicators (ALT, AST) and body oxidative stress indicators (MDA, LDH) decreasing significantly, and the activities of their antioxidant enzymes (CAT, SOD, GSH-Px) increasing significantly. Real-time fluorescent quantitative PCR results showed that the expression of Sod1 (3.58) and Gpx1 (7.14) in the liver of the Se-DD98 high-dose group was significantly up-regulated (by 16.41 and 10.19 times, respectively), and the expression of pro-inflammatory factor IL-1β (2.16) was significantly down-regulated (by 1.61 times). The mRNA levels of hepatic Sod1 (3.58) and Gpx1 (7.14) were significantly up-regulated respectively by 16.41 and 10.19 times in Se-DD98 high-dose group. In addition, both the high- and low-dose Se-DD98 groups could reduce X-ray-induced liver damage. Finally, the indices of the Se-DD98 high-dose group differed very little from the normal control group but differed significantly from the radiation control group. Therefore, Se-DD98 can alleviate X-ray-induced hepatic damage in mice through reducing the level of oxidative stress, boosting the activities of the antioxidant enzymes and inhibiting tissue inflammation.
    3  Kappaphycus alvarezii Prevents 5-fluorouracil-induced Intestinal Mucosal Injuryin Mice
    ZHANG Qing GAO Yuan CHENG Xiao-jie WANG Zhi-guang LI Chun-jun LIN Bi-yin TANG Qing-juan
    2021, 37(6):20-27. DOI: 10.13982/j.mfst.1673-9078.2021.6.1096
    [Abstract](247) [HTML](515) [PDF 3.06 M](593)
    Abstract:
    Herein, the in vivo protective effects of Kappaphycus alvarezii, dried using two different methods, over 5-fluorouracil (5-FU)-induced intestinal mucosal injury were explored. A total of 35 Balb/c mice were randomly divided into five groups: non-treated group (N), placebo treated group (M), treated with 5-aminosalicylic acid (5-ASA) group, treated with sun-dried K. alvarezii (SKA) group, and treated with air-dried K. alvarezii (AKA) group. Intestinal mucosal injury was induced by intraperitoneal injection of 5-FU, and the body weight (bw) and food intake of the mice were recorded daily. After the experiment, the thymus and spleen indexes were calculated. The jejunum histology was evaluated, including the villus length, crypt depth, and their ratio (V/C value). An enzyme-linked immunoassay was performed to detect the serum levels of the tumor necrosis factor (TNF)-α and interleukin-10. Overall, the weight gain rates of all groups were higher than that of the M group (-3.30%, -6.19%, -5.09%> -7.50%). Moreover, the weight and food intake reduction was significantly suppressed by day 3 of treatment (16.85, 16.83, 16.76>15.75 g, and 2.15, 2.18, 2.19>1.54 g). The thymus index of the 5-ASA group (5.92 mg/10 g b.w.) and the AKA group (6.81 mg/10 g b.w.) were slightly higher than that of the M group (5.67 mg/10 g b.w.). Histological analysis showed that, compared with the M group, all treated groups had improved jejunum, with significantly longer villus (332.58, 334.44, 350.75>252.09 μm) (p<0.01). Additionally, the V/C values of the 5-ASA (6.39) and AKA (6.35) groups were much higher than that of the M group (5.12) (p<0.05). Compared with the M group, the AKA group has significantly lower serum TNF-αlevels (470.73<580.69 pg/mL) (p<0.05) and remarkably higher interleukin-10 (306.11>287.41 pg/mL). Altogether, dried K. alvarezii, regardless of the drying methods used, can alleviate the manifestations of 5-FU-induced intestinal mucosal injury in mice.
    4  The Combination of Oyster Peptides, Soybean Peptides, Collagen Peptides, and Perilla Seed Peptides Promotes the Production of Male Hormones in Testicular Stromal Cells
    FANG Lei WANG Yu-chen MA Yong-qing ZHANG Hai-xin LU Lu WANG Jing GU Rui-zeng WEI Ying
    2021, 37(6):28-34. DOI: 10.13982/j.mfst.1673-9078.2021.6.1074
    [Abstract](329) [HTML](542) [PDF 1.75 M](748)
    Abstract:
    The amino acid composition of oyster peptides, soy peptides, collagen peptides and perilla peptides were analyzed. Oyster peptide, soy peptide, collagen peptide, and perilla seed peptide are compounded in different proportions to affect the secretion of testosterone, dihydrotestosterone and other male hormones in mouse leydig cells (TM3) and the oligopeptide combinations were screened for improving sexual function. Tetramethylazozol blue (MTT) colorimetric method was used to determine the effect of oyster peptides, soy peptides, collagen peptides, and perilla peptides on the survival rate of TM3 cells. ELISA was used to detect testosterone, dihydrotestosterone, cyclic GMP (cGMP) content, nitrate reductase method was studied to detect nitric oxide (NO) content, WST-8 method and thiobarbituric acid method were used to detect superoxide dismutase (SOD) enzyme activity and malondialdehyde (MDA) concentration, respectively. The results showed that the four oligopeptides were rich in arginine, especially the perilla peptide, which had the highest arginine content 10.02%. Compared with the control group, oyster peptide and perilla seed peptide (both at a concentration of 400 μg/mL, volume 2:1) had the best combined effect, and the promotion effects on testosterone, dihydrotestosterone, and cGMP were 50.64%, 143.69%, and 88.44%, respectively. In addition, they can also increase the NO concentration and SOD activity of TM3 cells, and reduce the concentration of TM3 cells; maintain a stable oxidizing environment in the cells. In summary, it is shown that the combination of oyster peptide and perilla seed peptide promotes the production of male hormones by TM3 cells and has the effect of improving male sexual function.
    5  Sedative and Hypnotic Effects of Agarwood Extract on PCPA-induced Insomnia Rats
    LI Shu-gen LIU Kun-dong WANG Hao-nan YAO Xiao-ling SU Ning
    2021, 37(6):35-42. DOI: 10.13982/j.mfst.1673-9078.2021.6.1031
    [Abstract](296) [HTML](238) [PDF 1.54 M](1288)
    Abstract:
    To explore the sedative and hypnotic effects of agarwood extract on PCPA (P-chlorophenylalanine, PCPA) - induced insomnia rats. Ninety SD rats were selected, 10 were used as the blank group, and the remaining 80 rats were used to establish insomnia rat models. They were divided into model group, agarwood volatile oil high-dose group, agarwood volatile oil low-dose group, agarwood alcohol extract high-dose group, and agarwood alcohol the low-dose extract group, the high-dose group of water extract of agarwood group, the low-dose group of water extract of agarwood group, and the diazepam group. Hypothalamic serotonin (5-HT), dopamine (DA), norepinephrine (NE), γ-aminobutyric acid (GABA), glutamic acid (Glu) content and GABAARα1, GABAARγ2, 5-HT1A relative expression of body mRNA were observed. After 6 days of administration, the 5-HT contents of hypothalamus in the high-dose volatile oil group, low-dose volatile oil group, high-dose alcohol extract group, and low-dose alcohol extract group were 4.85 ng/mL, 4.84 ng/mL, 4.81 ng/mL, 4.49 ng/mL, respectively. GABA contents were 1.46 ng/mL, 1.44 ng/mL, 1.41 ng/mL, 1.39 ng/mL, respectively, which were higher than the model group (p<0.05). The relative expression levels of GABAARα1 mRNA in the high-dose volatile oil group, low-dose volatile oil group, and high-dose alcohol extract group were 0.94, 0.84, 0.85, respectively, all of them were higher than the model group (p<0.05). The relative expression of GABAARγ2 mRNA were 0.74, 0.70, 0.73, respectively, and the relative expression of 5-HT1A receptor mRNA were 0.81, 0.79, 0.74, respectively, all of them were higher than the model group (p<0.05). Agarwood extract has a certain sedative and hypnotic effect on PCPA-induced insomnia rats.
    6  Improved Sleep in Mice Treated with Wolfiporia extensa Extracts Produced Using Different Solvents
    SUN Meng-na YE Hui-yu LI Qiang-ming ZHANG Yu-ying YANG Si-ling WEI Chun-ting LUO Jian-ping
    2021, 37(6):43-49. DOI: 10.13982/j.mfst.1673-9078.2021.6.1084
    [Abstract](205) [HTML](1047) [PDF 931.16 K](597)
    Abstract:
    The extent of the improvements in sleep habits of mice treated with Wolfiporia extensa extracts obtained using different solvents were compared using sleep tests, prolonged pentobarbital-induced sleeping time measurements, below-threshold dose pentobarbital-induced hypnosis tests, and pentobarbital-induced sleep latency tests. Changes in the 5-HT, GABA, and DA content in mouse brain tissues were monitored to explore the mechanism by which Wolfiporia extensa extracts obtained using different solvents improve sleep in mice. The results reveal that, the group treated with high-dose aqueous extracts (112.7 mg/kg) show the greatest improvement in their sleep (p<0.05) compared to the blank control. This group is followed by the groups treated with high-dose ethanol extracts (100.8 mg/kg), high-dose polysaccharides (15.6 mg/kg), low-dose aqueous extracts (56.4 mg/kg), and low-dose ethanol extracts (50.4 mg/kg). Analysis also revealed that low-dose polysaccharides (7.8 mg/kg) (p>0.05) treatment induced very little change in the sleep habits of these animals. More specifically, the sleeping time of the mice treated with high-dose aqueous extracts increased remarkably from 1829.67 s to 2979.83 s, and the incidence rate rises from 10% to 60%. In addition, sleep latency was markedly reduced, from 2033.50 s to 1486.25 s, and the sleep improvements associated with these Wolfiporia extensa extracts were shown to coincide with increases in the GABA and 5-HT content and reductions in the DA content in the brain tissues of treated animals. The 5-HT and GABA content reached up to 26.96 pg/mg and 817.40 pg/mg, respectively, in the animals treated with high-dose aqueous extracts while the DA content dropped to 3.41 pg/mg. This data reveals that high-dose aqueous Wolfiporia extensa extracts are the best option for improving sleep in mice, and that this improvement is closely associated with the regulation of GABA, 5-HT, and DA in the brain.
    7  Protective Effect of Total Flavonoids of Penthorum chinense Pursh on Acute Chemical Liver Injury Induced by Carbon Tetrachloride in Mice
    FU Man-ling JIU Hong YUAN Ye-fei
    2021, 37(6):50-56. DOI: 10.13982/j.mfst.1673-9078.2021.6.1054
    [Abstract](150) [HTML](259) [PDF 2.30 M](593)
    Abstract:
    To study the protective effect of total flavonoids of Penthorum chinense Pursh on acute chemical liver injury induced by carbon tetrachloride in mice, in this study, 50 SPF Kunming mice were divided into five groups including normal group, model group, bifendate dropping pills group (150 mg/kg), total flavonoids of Penthorum chinense Pursh in high and low dose groups (800, 400 mg/kg). The mice were intragastrically administered with drugs or distilled water according to the body weight once a day for 21 days. One hour after the last administration, the other groups except the normal group were treated with the intraperitoneal injection of 1% CCl4 olive oil solution (10 mL/kg). Then all mice were killed after 16h, and the serum was collected for the determination of ALT, AST, ALP and LDH levels by automatic biochemical analyzer. Liver was triturated for detecting TNF-α, IL-6, SOD and MDA by ELISA method. The pathological changes of liver tissue were observed by HE staining. Compared with the model group, the activities of serum ALT, AST, ALP and LDH in high dose group were respectively reduced by 86.01%, 91.03%, 25.50% and 95.54%, while in low dose group were respectively decreased by 76.06%, 84.91% and 93.87%; in high dose group, the contents of TNF-α, IL-6 and MDA in liver tissue were respectively decreased by 24.89%, 20.91% and 39.45%, while SOD activity increased by 48.22%; in low dose group, the contents of IL-6 and MDA in liver tissue were respectively decreased by 18.60% and 25.62%, while SOD activity increased by 42.23%; and the pathological conditions of liver were significantly improved in high and low dose groups. The results suggest that total flavonoids of Penthorum chinense Pursh exhibits the protective effect on acute chemical liver injury induced by CCl4 in mice, and the mechanism of action may be associated with its inhibition of the oxidative stress reaction and the inflammatory reaction.
    8  Chitooligosaccharides Regulate Dyslipidemia and Oxidative Stress in NOD Mice
    CHENG Qin-yuan YAN Lin MA Ming WU Xiao-yu
    2021, 37(6):57-62. DOI: 10.13982/j.mfst.1673-9078.2021.6.0956
    [Abstract](236) [HTML](212) [PDF 3.39 M](600)
    Abstract:
    To explore the possible mechanism underlying the ability of chitooligosaccharides (COS) to delay the progression of spontaneous diabetes in NOD mice, the effects of different doses of COS on dyslipidemia and oxidative stress were studied. After continuous intragastric administration of different doses of COS for 28 weeks, the indicators including blood glucose, blood lipids and oxidative stress of mice in each group were determined, and the histological examination was carried out. The results showed that COS at each dose could delay the time for NOD mice to enter the prediabetic and diabetic phases, and extend the time period of prediabetic phase to 10 W, 13 W and >16 W, respectively (9 w for the control group); COS at the three doses could decrease the level of T-CHO by 37.10%, 46.68% and 62.72%, respectively (p<0.01), level of TC by 35.01%, 45.06% and 60.62%, respectively (p<0.01), and level of LDL-C by 31.21% (p<0.05), 51.68% and 72.48%, respectively (p<0.01), although insignificant effect of COS on HDL-C was found. These results indicate that COS can improve dyslipidemia, increase the specific activities of SOD and GSH-Px in the liver tissues of mice (especially increased by 94.69% and 167.59%, respectively, in the COS-H group; p<0.01), and decreased the MDA concentration (by 48.41%; p<0.05), thereby reducing effectively oxidative stress-induced oxidative damage. Histological examination showed that COS increased the pancreatic coefficient, decreased gradually the liver coefficient, reduced fat vacuoles, providing a certain protective effect on the integrity of pancreatic tissue. According to these results, COS can delay the development of spontaneous diabetes through regulating dyslipidemia, resisting oxidative stress in the body caused by abnormal blood sugar, and protecting the tissues.
    9  Genome Functional Annotation Analysis of the Genetic Basis for the Superior Brewing Performance of Aspergillus oryzae ZA189
    HOU Sha WU Chang-zheng PAN Li WANG Bin
    2021, 37(6):63-71. DOI: 10.13982/j.mfst.1673-9078.2021.6.0673
    [Abstract](256) [HTML](328) [PDF 3.64 M](608)
    Abstract:
    Aspergillus oryzae ZA189 was obtained via traditional mutagenesis using A. oryzae Huniang 3042 as the starting strain, and possessed high activities of neutral protease, amylase and glutaminase. To further investigate the genetic background of A. oryzae ZA189 and evaluate its performance as the strain for soy sauce brewing, the sequencing, assembly and functional annotation of the genome of A. oryzae ZA189 was achieved using the new generation nanopore sequencing technology in this study. The length of the obtained genome was 36.89 Mb, having 16 Scaffolds and sequencing depth of 132.05×. Gene annotation showed that the A. oryzae ZA189 genome contained 263 tRNA genes and 72 rRNA genes, which was the genetic basis for high-efficiency A. oryzae protein expression. KOG and KEGG annotations illustrated that A. oryzae ZA189 possessed strong capabilities of energy synthesis, protein synthesis and secondary metabolite synthesis, which formed the genetic foundation for its wide application in the fermentation industry and the production of multiple flavor functional substances. Annotation of carbohydrate-active enzymes (CAZys) and proteases showed that A. oryzae ZA189 genome contained as many as 330 glycoside hydrolase (GH) genes and 30 protease genes, which is the key performance indicator of A. oryzae ZA189 as a soy sauce brewing strain in degrading soybean proteins and carbohydrates. Through this research, a more in-depth understanding of the genome of the soy sauce brewing strain, A. oryzae ZA189. Functional annotation of protein expression system genes, secondary metabolism genes, and CAZys and proteases provides a theoretical support for guiding its application in soy sauce brewing.
    10  A Molecular Dynamics Simulation Study on the Mechanism Underlying the Interaction between Chitooligosaccharides and Lipase
    YANG Bao-yan ZHENG Dun-jin HE Kang-ping JIANG Wen-hao SHEN Rui-feng LYU Yong-si LUO Xing-lin LUO Jia-wei CHEN Yin-xi ZHENG Shao-peng HAO Jin-heng CHEN Zi-shi CAO Shi-lin
    2021, 37(6):72-81. DOI: 10.13982/j.mfst.1673-9078.2021.6.0902
    [Abstract](319) [HTML](766) [PDF 3.71 M](748)
    Abstract:
    Chitosan and its derivatives have a wide range of applications in the field of enzyme immobilization. Understanding the mechanism of the interaction between enzymes and chitosan derivatives at the molecular level is of great significance to the design and application of related biocatalysts. In this study, molecular dynamics simulation was used to investigate the interaction of Candida Antarctica Lipase B (CaLB) and oligo-chitosan (OCTS), and molecular docking of lipase and catalytic substrate was conducted to study the binding conformation and affinity for the catalytically active centre of CaLB and substrate. The results showed that both electrostatic and L-J interactions between CaLB and OCTS play important roles in the initial stage of the self-assembly process. After the simulation process, the CaLB-OCTS assembly contained 14 OCTS molecules and 1 CaLB molecule. The electrostatic interaction and hydrogen bond interaction existed between CaLB and OCTS. During the simulation, the L-J potential energy and Coulomb potential energy decreased by about 1480 KJ/mol and 2324.0 KJ/mol, respectively, with the average number of hydrogen bonds between CaLB and OCTS increasing from 0 to about 17, and the accessible area of both CaLB and OCTS reducing by 20 nm2 due to the interactions. The analysis of secondary structure showed that compared to the free enzyme, the beta-sheet content of CaLB-OCTS increased from 9.78% to 12.62%, and the content of random coil loop decreased from 49.21% to 46.50%. Moreover, the CaLB-OCTS retained its original protein skeleton structure and the conformation of the active site pocket.
    11  Purification and Enzymatic Properties of Esterase Produced by Aureobasidium
    ZHONG Li-ming CAO Xin-zhi LI Shu-yuan ZHANG Kai-zheng HU Qin
    2021, 37(6):82-88. DOI: 10.13982/j.mfst.1673-9078.2021.6.1014
    [Abstract](233) [HTML](292) [PDF 1.96 M](554)
    Abstract:
    The positive effects of esterification enzyme on improving the yield of Luzhou-flavor liquor, high quality liquor and reducing the amount of qu were analyzed. A strain producing esterifying enzyme from Wuliangye Daqu was studied. It was identified as Lichtheimia ramosa. The supernatant of esterase fermentation broth was treated by ammonium sulfate precipitation, dialysis, ultrafiltration centrifugation, DEAE-cellulose-52 ion exchange chromatography and Sephadex G-100 gel filtration chromatography. After electrophoresis, the electrophoresis purity reached 35.66 times, and the activity recovery rate was 2.54%. The molecular weight of 12% SDS PAGE was about 43000 u. The results showed that the optimum temperature of esterification enzyme was 40 ℃ and it was sensitive to heat. Only 30% enzyme activity remained after treatment at 60 ℃ for 30 min. The optimum pH range of enzyme was 6~8, and the optimum pH was 7.5. Fe2+, Zn2+, Mn2+ and Cu2+ had significant inhibition on esterification enzyme. The esterification enzyme has the characteristics of high efficiency, alkali resistance and heat sensitivity, avoiding the influence of high inactivation on the product, and has a good application prospect in wine making, food, medicine and textile industries.
    12  Optimization of the Fermentation Technology of Black Tea Vinegar
    WEI Jian-min CHEN Yan CHEN Li LU Hong-mei
    2021, 37(6):89-97. DOI: 10.13982/j.mfst.1673-9078.2021.6.1030
    [Abstract](408) [HTML](258) [PDF 1.02 M](629)
    Abstract:
    In order to determine the best technological parameters of black tea fruit vinegar, black tea and pomegranate were used as raw materials in this study, yeast and acetic acid bacteria were inoculated for fermentation. Based on the single factor experiments, the orthogonal experiment of alcohol fermentation stage was carried out with tea juice ratio, yeast inoculation amount and sugar content as factors, and the orthogonal experiment of acetic acid fermentation stage was carried out with the initial alcohol precision, acetic acid bacteria inoculation amount and fermentation temperature as factors. The results showed that the optimal fermentation conditions were as follows: in alcohol fermentation stage, microwave extraction was 4 minutes, the ratio of tea to water was 5:100, the ratio of tea to juice was 1:5, yeast inoculation amount was 0.2%, sugar addition amount was 16 g/100 mL; in acetic acid fermentation stage, bottling amount was 30%, initial ethanol content was 7% vol, acetic acid bacteria inoculation amount was 12%, fermentation at 30 ℃. Under the optimized conditions, the total acid ofblack tea fruit vinegar was 6.48 g/100 mL, and the content of tea polyphenols was 397.85 mg/L. It was rich in 16 kinds of amino acids, and the total content was 5.93 mg/mL, among which the essential amino acids accounted for 38.45% of the total content. Its color was bright, aroma was harmonious, flavor was unique, the physical and chemical and health indicators met the relevant national standards. It provided a theoretical basis for the development and industrial production of black tea vinegar.
    13  Optimization of Culture Medium for High-yield Astaxanthin in Haematococcus pluvialis
    SUN Shuang ZHANG Ting FANG Yan HU Sha-sha HUANG Si-qi YE Qi-ran LAN Li-qiong QING Ren-wei
    2021, 37(6):98-107. DOI: 10.13982/j.mfst.1673-9078.2021.6.1000
    [Abstract](251) [HTML](624) [PDF 4.76 M](689)
    Abstract:
    In this study, an optimal medium for cell proliferation of Haematococcus pluvialis HP1 during the green cell growth stage was selected from five media. Furthermore, the organic and inorganic carbon and their concentrations in the selected medium were also optimized respectively. Based on this, the single factor experiment and central composite design (CCD) response surface analysis were utilized to optimize the stress condition of astaxanthin accumulation with light intensity, initial pH value and inducement time as experimental factors. The results indicated that the biomass of HP1 could reach a higher level in medium A and OHM, and the optimal concentration of organic carbon source and inorganic carbon source was 0.5 g/L. The optimal stress conditions for HP1 astaxanthin accumulation were: light 145 μmol/m2·s, initial pH 7.0, and induction time 9 d. Under the conditions described above, the maximum yield of astaxanthin reached 5.74 mg/L in NaAC culture system and 4.25 mg/L in NaHCO3 culture system, respectively. This study can provide a scientific basis for further increasing the astaxanthin yield of Haematococcus pluvialis.
    14  Study on the Relationship between Water Absorption Speed and Protein Composition Characteristics of Wheat Flour
    LIN Jiang-tao GUO Xiao-dan SU Dong-min
    2021, 37(6):108-114. DOI: 10.13982/j.mfst.1673-9078.2021.6.1036
    [Abstract](253) [HTML](303) [PDF 2.20 M](767)
    Abstract:
    The difference in the physical and chemical properties of protein causes the difference in the water absorption speed of wheat flour. Taking the wheat flours with different water absorption speed as research objects, the crude protein content, gluten protein content, wet gluten content, gluten index, sedimentation value, gluten swelling index, amino acid content and the internal micro network structure of the dough were measured in this paper. Results showed that the water absorption speed of wheat flour increased from 0.17 g/s to 3.87 g/s when the crude protein content was reduced from 13.25% to 6.98%. Besides, when the gliadin content decreased from 4.02% to 1.53% and the glutenin content decreased from 5.28% to 2.77%, the quality of the gluten membrane decreased, making the dough easier to shape, and increasing the water absorption speed of wheat flour. Meanwhile, the water absorption speed increased significantly with the decrease of wet gluten content, and the gluten index had no significant effect on the water absorption speed. The sedimentation value decreased from 69.50 mL to 32.50 mL, and the gluten swelling index decreased from 5.24% to 3.33%, both of which were significantly negatively correlated with the water absorption speed (p<0.05). The amino acid content affected the molecular force of the protein. The cysteine content was reduced from 0.14% to 0.04%, resulting in a decrease in the formation of disulfide bonds, and thereby affecting the protein structure. When the internal micro network structure of the dough was tight, the greater the resistance of the flour to agglomespeed, the lower the water absorption speed.The results showed that there was a negative correlation between protein properties and water absorption speed of wheat flour.
    15  Aggregation State and Structural Properties of Zein in Different Solvents
    LAI Chan-juan WU Lei-yan HU Lin-fang TU Jin DONG Wu-hui
    2021, 37(6):115-123. DOI: 10.13982/j.mfst.1673-9078.2021.6.0258
    [Abstract](693) [HTML](643) [PDF 4.50 M](857)
    Abstract:
    The dissolution, aggregation, and structural properties of zein in different solvents (methanol, ethanol, isopropanol, acetic acid, and acetone) were studied using ultraviolet spectroscopy, Fourier-transform infrared spectroscopy, fluorescence spectroscopy, and scanning electron microscopy. The surface hydrophobicity of the zein protein film was determined by static contact angle measurements. Zein showed good dissolution in 100% acetic acid, 80% ethanol, and isopropanol solutions, and the resulting solutions exhibited high light transmittance. A maximum particle size of 5230.16 nm and a minimum particle size of 25.52 nm were obtained when zein was dissolved in 70% acetic acid and 80% ethanol solutions, respectively. Zein dissolved in isopropanol showed high fluorescence absorption. Zein dissolved in 80% acetic acid and ethanol solutions showed contact angles of 88.90° and 86.60°, respectively, with the containers, suggesting neutral wettability and suitability of the solutions for stabilizing oil-water interfaces. The air-side contact angle of the zein film formed in 80% methanol solution was the largest (72.10°). These results revealed that the solvent concentrations affected the water-protein interactions, thereby resulting in different aggregation degrees of the proteins. In addition, differences in the solvent polarity and electronegativity also affected the particle size, surface hydrophobicity, and spatial conformation of the protein by changing the hydrophobic interactions between the molecules and formation of hydrogen and disulfide bonds.
    16  Improving the Solubility of Soybean Protein Isolate by High-Intensity Ultrasound
    LI Xiao-xiao SUN-WATERHOUSE Dong-xiao ZHANG Yi SUN Wei-zheng
    2021, 37(6):124-128. DOI: 10.13982/j.mfst.1673-9078.2021.6.0718
    [Abstract](317) [HTML](261) [PDF 1.47 M](1081)
    Abstract:
    In this study, the effect of high-intensity ultrasonic treatment on the solubility of the 3% (W/V) and 5% (W/V) commercial soy protein isolate (SPI) was investigated. The effects of high-intensity ultrasonic treatment on the surface hydrophobicity, particle size and electrophoretic mobility of SPI were examined to analyze the mechanism underlying the influence of SPI’s structural changes on its solubility. Results showed that the high-intensity ultrasonic treatment (200~800 W) could improve significantly the solubilities of the commercial SPI products at two mass concentrations. With the increase in ultrasonic power, SPI solubility increased first and then decreased. The ultrasonic treatment at 400 W could lead to the maximum solubility (47.86%), which increased by 87.4% compared to the control. After each of the ultrasonic treatments (except for that at 800 W), the solubility of 3% (W/V) SPI was higher than that of 5% (W/V) SPI. The results of surface hydrophobicity and particle size showed that the low-power ultrasonic treatment could induce partial depolymerization of SPI aggregates, reduce protein’s surface hydrophobicity and particle size thereby increasing solubility of SPI. However, high-power ultrasonic treatment could induce the re-formation of insoluble aggregates, leading to the decrease of SPI solubility. Results of SDS-PAGE showed that the ultrasonic treatment did not destroy the structure of protein subunits. The results showed the optimal improvement of the solubility of the 3% (W/V) SPI obtained by the ultrasonic treatment at 400 W. This work provides guidance for improving the solubility of commercial SPI.
    17  The Transfer and Distribution of Tetrodotoxin in Takifugu obscurus and the Activity Analysis of Related Metabolic Enzymes
    LU Yi-bo ZENG Jun-jie CHEN Si WANG Rui-rui ZHANG Xiao-jun
    2021, 37(6):129-135. DOI: 10.13982/j.mfst.1673-9078.2021.6.1055
    [Abstract](261) [HTML](246) [PDF 2.31 M](773)
    Abstract:
    In order to study the transfer and distribution of tetrodotoxin (TTX) in Takifugu obscurus and its activity on liver metabolizing enzymes, the oral gavage administration method was used in the experiment. The concentration changes of TTX in different tissue were measured by ultra performance liquid chromatography tandem mass spectrometry, and the activity of metabolic enzymes in the liver was also measured. The results were as follows, during the short-term metastasis distribution period after TTX instillation, the concentration of TTX in the gonads increased from 19.00 ng/g to 42.02 ng/g, and the accumulation rate was 121%. The TTX concentration was fluctuated in the liver. At 4 h, the highest TTX concentration was 11.43 ng/g, at 48 h, the lowest TTX concentration was 1.27 ng/mL. The TTX contents in the tissues of skin, muscle, blood, intestine, and gills were highest at 2 h, which were 7.43 ng/g, 1.15 ng/g, 11.44 ng/g, 9.77 ng/g, and 31.75 ng/g, respectively. The highest TTX concentrations in the spleen, kidney and gallbladder tissues were 5.05 ng/g, 6.54 ng/g, and 9.06 ng/g at 2 h, 4 h, and 6 h, respectively. In addition, the test results of liver metabolic enzyme activity showed that TTX significantly increased the activities of lactate dehydrogenase, aspartate aminotransferase and alanine aminotransferase (p<0.05). And it had a significant inhibitory effect on two phosphatase activities in the early stage (p<0.05), and then returned to normal levels. Theresults indicate thatthe transfer and distribution of TTX in Takifugu obscurus has tissue specificity, and can promote and inhibit the activities of different metabolic enzymes in the liver.
    18  Atomized Calcium Chloride and Salicylic Acid Fumigation Prevents Postharvest Quality Deterioration and Antioxidant Capacity Reduction of Jujube (Zizyphus jujuba Mill.)
    WANG Xia-wei DENG Hao ZHANG Ping WANG Man LI Zhe WEI Jia WU Bin
    2021, 37(6):136-143. DOI: 10.13982/j.mfst.1673-9078.2021.6.1115
    [Abstract](169) [HTML](198) [PDF 3.49 M](623)
    Abstract:
    Herein, the synergistic effects of atomized calcium chloride (CaCl2) and salicylic acid (SA) fumigation on the postharvest quality and antioxidant capacity of Zizyphus jujuba Mill., commonly known as Dongzao or winter jujube, were evaluated. Overall, treatment with 2% CaCl2+100 mmol/L SA showed to slowdown the color change and inhibit the softening process of jujubes, as well as it prevented the weight loss rate and titratable acid (TA) content, as compared with 2% CaCl2 or 2% CaCl2+200 mmol/L SA treatments. Specifically, on day 6 upon treatment, the a factor value, hardness, weight loss rate, and TA content were 0.70, 9.24 N, 1.76%, and 0.37%, respectively, for jujubes treated with 2% CaCl2+100 mmol/L SA, indicating that atomized CaCl2+SA fumigation could effectively preserve the postharvest quality and color of the fruits. Overall, 2% CaCl2+100 mmol/L SA treatment provides better preservation effects than 2% CaCl2 and 2% CaCl2+200 mmol/L SA treatments. Moreover, the ascorbic acid content of the jujubes treated with 2% CaCl2+100 mmol/L SA was of 2.65 mg/g formula weight (FW), with a total phenol and flavonoid contents of 4.83 and 1.38 mg/g FW, respectively. These values were 1.35-, 1.21-, and 1.31-times higher, respectively, than those of the CK group. 2,2-Diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid scavenging abilities, and the ferric reducing antioxidant power of the 2% CaCl2+100 mmol/L SA-treated jujubes were 51%, 23%, and 40% higher than those of the CK group, indicating that atomized CaCl2+SA fumigation improved the antioxidant capacity of the jujubes, thereby extending their shelf-life. Therefore, CaCl2+SA fumigation can pave the way for new techniques to enhance storage and transportation postharvest outcome of winter jujubes.
    19  Comparison of Different Preservation Techniques for Lotus Seed Pods at Room Temperature
    ZHANG Duo
    2021, 37(6):144-149. DOI: 10.13982/j.mfst.1673-9078.2021.6.0288
    [Abstract](210) [HTML](386) [PDF 2.33 M](711)
    Abstract:
    To identify the best method to preserve fresh lotus seed pods at room temperature, the suitability of three techniques, namely, food wrapping, fruit wax coating, and 1-methylpropene (1-MCP) treatment, was examined. Based on the evaluation parameters of sensory quality, browning index, weight loss rate, respiration intensity, soluble solid content, malondialdehyde (MDA) content, and polyphenol oxidase (PPO) activity, the most optimal preservation technique at room temperature was confirmed. At room temperature, the sensory quality scores of lotus seed pods of the control, food wrap preservation, fruit wax coating, and 1-MCP treatment groups were 7.0, 7.7, 7.8, and 8.3, respectively, and the sensory quality scores of lotus seeds were 8.0, 8.3, 8.6, and 8.7, respectively. The browning indexes of the lotus seed pods were 0.50, 0.45, 0.30, and 0.20, respectively. The weight loss rates of the lotus seeds were 7.46%, 5.58%, 4.28%, and 4.43%, respectively. The soluble solid contents decreased by 66.82%, 45.83%, 40.19%, and 37.87%, respectively. The MDA contents were 1.53, 1.33, 1.19, and 1.03 μmol/L, respectively. During storage, the maximal respiratory intensities were 37.2, 36.1, 35.9, and 34.1, respectively. The maximal PPO activities were 116, 93, 81, and 52, respectively. All of these suggest that food wrapping preservation, fruit wax coating, and 1-MCP treatment could inhibit the respiration of lotus seed pods and slow down the decrease of water and soluble solid contents. 1-MCP treatment method demonstrated the best preservation effects. Therefore, 1-MCP treatment could be an effective preservation technique to delay the postharvest senescence and prolong the shelf life of lotus seed pods and seeds.
    20  Changes in the Quality of Beef Ball Products Reheated by Water-Bath Cooking
    CHENG Jun-hu LIANG Han-ni HAN Zhong SUN Da-wen YU Xu-cong ZHANG Tian-yi
    2021, 37(6):150-158. DOI: 10.13982/j.mfst.1673-9078.2021.6.0391
    [Abstract](320) [HTML](520) [PDF 2.06 M](672)
    Abstract:
    In order to explore the changing trend of the quality of meatball products reheated by water-bath cooking, Chaoshan beef balls were used as the experimental object subjected to reheating with boiling water and cold water, to determine their sensory attributes, nutrient contents (water, protein, starch), texture, flavor change and microstructure. The results show that with the increase of reheating time, the moisture content of beef balls increased first and then decreased, accompanied by an increase in protein content. Under the cold-water reheating conditions, the protein content increased significantly (by 14%~29%), while the starch content remained essentially unchanged. A total of 28 volatile components were identified in beef balls before and after cooking, among which the number and content of sulfur-containing compounds were the highest. After being reheated in boiling water, beef balls had the highest content of volatile substances (11053.24 μg/kg). Microstructure analysis showed that the meatballs shrank with their pore size becoming smaller after water-bath cooking. As the cooking time extended, the pore size became larger and denser. The comparison of the reheating modes revealed that reheating meat balls in boiling water for 5.5 min was the best water-bath cooking method, making the meat balls having a core temperature and highest sensory score, up to 90 ℃, with a moisture content, protein content and starch content of 69.78%, 14.43% and 4.41%, respectively.
    21  Simplifying the Processing of Broken Green Tea from Hubei Province and Improvement of Physico-chemical Quality
    YE Fei GONG Zi-ming GUI An-hui WANG Sheng-peng LIU San-yun YAN Feng HU Shan-bin GAO Shi-wei
    2021, 37(6):159-166. DOI: 10.13982/j.mfst.1673-9078.2021.6.0914
    [Abstract](218) [HTML](258) [PDF 1.68 M](607)
    Abstract:
    In order to improve the quality of broken green tea of Hubei Province and reduce production costs, the current broken green tea processing procedure was used as a control to compare and analyze the sensory scores, color quality, physico-chemical components, aroma components and production costs of the samples prepared by different processes. The results showed that the broken green tea prepared by the lightly simplified process (treatment 3) had the highest sensory scores (79.8, which was 1.45 points higher than the control). Compared with the control, such dried tea had significantly increased brightness (p<0.01), and its tea infusion had an increased hue value (by 132%, p<0.01), higher catechin quality index (p<0.01), increased contents of aroma components (phenylacetaldehyde, (E,E)-2,4-heptadienal, safranal, nerol, cis-linalool oxide, β-ionone, trans-geranylacetone and α-copaene), and reduced labor cost (by 25%). Taken together, the lightly simplified process improved effectively the quality of broken green tea and reduced the production costs, which could be used as an effective technical means to improve the quality of broken green tea.
    22  Effect of Nano-silica on the Stability and Efficacy of a Stomach-protecting Solid Drink
    YUAN Er-dong LIU Liang-yun HUANG Min REN Jiao-yan
    2021, 37(6):167-174. DOI: 10.13982/j.mfst.1673-9078.2021.6.0612
    [Abstract](209) [HTML](430) [PDF 1.17 M](603)
    Abstract:
    In order to explore the feasibility of applying nano-scale food additives in functional foods, this research studied the anti-caking effect of silica (SiO2) and nano-silica (nSiO2) in stomach-protecting solid drink, and analyzed their effects on the efficacy of the resultant beverages. Added different proportions of SiO2 or nSiO2 to the stomach-protecting solid beverage prepared with raw materials including the extracts of astragalus, poria and puerarialobata as well as the polysaccharides of Hericium erinaceus. Through accelerated storage experiments, the stability of the solid beverages was evaluated using angle of repose and bulk density as the indices. The gastric protection efficacy was evaluated using normal human gastric mucosal epithelial cells (GES-1) and ethanol injury model. The results showed that in the addition range of 0~1.50%, the anti-caking effects of SiO2 and nSiO2 gradually increased with the increase of addition proportion: the angles of repose of SiO2 and nSiO2 at 1.50% were152 ° and 153 °, respectively, and the bulk densities were 2.17 mg/mL and 2.33 mg/mL, respectively (which were significantly different from those of the blank control group (p<0.05)), although there was no significant difference in the anti-caking effect between SiO2 and nSiO2. A 2-h induction with 1.00 mol/L of ethanol led to the construction of an ethanol-damaged GES-1 cell model. The results showed that the addition of SiO2 and nSiO2 has no adverse effect on the efficacy of stomach-protecting solid beverages. At the concentration of 31.25 μg/mL, nSiO2 also helped to enhance its promoting effect on the proliferation of GES-1 cells and preventive effects on the ethanol-damaged GES-1 cells, with the cell survival rates as 166% and 110%, respectively (which were significantly higher than those of the blank control group (100%; p<0.05)). Therefore, nano-silica has great application prospects in food production and the development of functional foods.
    23  Friction Analysis of Underground Silos during Backfilling
    LIU Hai-yan XU Xiang-nan ZHANG Hao WANG Zhen-qing YANG Jin-ping MA Tian-he
    2021, 37(6):175-183. DOI: 10.13982/j.mfst.1673-9078.2021.6.1094
    [Abstract](125) [HTML](242) [PDF 2.54 M](574)
    Abstract:
    To investigate the variations in friction in underground silos due to groundwater during backfilling, three silo models, namely, A, B, and C, with three different diameters of 400 mm, 500 mm, and 600 mm were designed. All these models were composed of 2 mm thick steel plates with a bottom inclination angle of 35°. Six water injection tests were conducted under two different backfilling conditions. The experimental results demonstrate that when there are sudden displacement changes at the top of the silos, the water levels of models A, B, and C are 41.40 mm, 39.80 mm, and 35.00 mm, respectively, under backfilling condition a. Meanwhile, the water levels become 40.60 mm, 39.00 mm, and 33.10 mm under backfilling condition b (p<0.05). In the stationary phase, the reaction forces at the top of the models A, B, and C remain unchanged. In the floating phase, the reaction force at the top of the silos abruptly changes. Under backfilling condition a, the water level-reaction force curves of models A, B, and C result in inflection points at water levels of 37.10 mm, 27.00 mm, and 21.00 mm, respectively. Under condition b, the inflection points are observed at water levels of 37.10 mm, 30.05 mm, and 24.00 mm, respectively (p<0.05). Because of the static frictional force, the displacement changes measured by the gauge lag behind the variation in reaction forces. In the stationary phase, under condition a, the maximum static frictional force of the models is 206.17 N, 316.81 N, and 364.16 N, respectively. These values are all greater than those under condition b, which are 197.61 N, 310.82 N, and 352.96 N, respectively; yet, the water levels of the models under two different conditions increase at nearly the same rate (p<0.05). In the floating phase, frictional force reduces rapidly under both conditions and then levels off gradually. In actual engineering projects, favorable anti-floating underground silo designs can be adopted by integrating the frictional force variations in the floating phase.
    24  Drying Behavior of Lotus Seeds, and Adhesive Properties of the Flour Processed in a Rotary Dryer
    XU Jian-guo XU Gang SHI Jin-song ZHANG Sen-wang GU Zhen LIU Yun-fei
    2021, 37(6):184-192. DOI: 10.13982/j.mfst.1673-9078.2021.6.1113
    [Abstract](317) [HTML](283) [PDF 2.58 M](606)
    Abstract:
    To determine the characteristic behaviors of lotus seeds during drying and assess the quality of the flour produced from dried lotus seeds using a rotary dryer, lotus seeds with an initial moisture content of ≈63% to 10% wet basis (w.b.) were dried at controlled temperatures of 60, 70, 80, and 90 ℃ with rotational speeds of 0.5, 1, and 1.5 r/min. The adhesive properties of the lotus seed flour were investigated using a rapid visco-analyzer (RVA). The results indicate that the drying process is prolonged by rotary drying. No constant-rate drying phase was found. Strong influences of temperature on the drying process were evident (p≤0.05). By contrast, rotational speed did not significantly affect the drying process (p>0.05). Estimates predicted using the Cavalcanti-Mata model were in good agreement with the experimental results (R2>0.999, E%<5.00%, RMSE<0.01). The effective moisture diffusivity of lotus seeds was between 3.94×10-10 and 1.00×10-9 m2/s. This increases as drying temperature increased from 60 to 90 ℃. The drying process considerably affects the adhesive properties of the flour obtained. Peak and final viscosities were significantly lower when the lotus seeds were dried at higher drying temperatures. The corresponding values at 60 ℃ were 1676.33 cP and 2228.00 cP, respectively. Those at 90 ℃ were 1268.00 cP and 1909.33 cP, respectively. The results suggest that lotus seed microstructures change with drying temperature due to starch gelatinization during rotary drying. The surfaces of lotus seeds dried at 60 ℃ are more porous, while more densely packed structures are noted in lotus seeds dried at 90 ℃. Lotus seed surfaces are denser if they are dried at higher temperatures because of changes in their microstructures. Compared to oven drying, rotary drying produces lotus seeds with more uniform moisture content, and a maximum variability in moisture content of only 4.59%. This study may aid in development of high-efficiency and high-quality lotus seed drying techniques and applications of lotus seed flour within the food industry.
    25  Optimizing the Extraction of Active Compounds from Morus macroura and the Evaluation of Their Antioxidant Capacities
    HUANG Xue-jiao WU Yuan-hui WU Juan CHI Yuan-long HE Qiang REN Yao
    2021, 37(6):193-200. DOI: 10.13982/j.mfst.1673-9078.2021.6.1081
    [Abstract](193) [HTML](242) [PDF 2.42 M](650)
    Abstract:
    A combination of ultrasound-assisted and ethanol extraction was used to simultaneously isolate several active compounds, including polyphenols, flavonoids, and polysaccharides, from Morus macroura. The relative content of each these three classes of active compound where then used as indicators to explore the effects of various factors, including ethanol concentration, raw material-to-solvent ratio and extraction temperature and duration, on the efficacy of compound extraction using single-factor experiments. Orthogonal tests based on the experimental results were conducted to optimize the extraction parameters. In addition, the antioxidant capacities of the extracts were also analyzed. The results of these assays reveal that the most favorable conditions for extracting active components from Morus macroura are as follows: an ethanol concentration of 60%, a raw material-to-solvent ratio of 1:35 g/mL, an extraction temperature at 45 ℃, and an extraction duration of 70 min. Under these conditions, the active component extraction rate reaches up to 20.49%. In particular, the average polyphenol, flavonoid, and polysaccharide content increased to 12.13 mg/g, 1.79 mg/g, and 58.74 mg/g, respectively. The 50% inhibitory concentrations (IC50) of the mulberry leaf extracts against DPPH, hydroxyl, and superoxide anion free radicals were also shown to increase to 0.06 mg/mL, 3.13 mg/mL, and 1.15 mg/mL, respectively, when using these extraction conditions. Thus, Morus macroura extracts can produce promising antioxidant effects when produced using optimized extraction conditions.
    26  Optimizing Antioxidant Peptide Preparation using the Proteolysis of the Prolamins Produced by Prunus dulcis var. amara
    XU Yang-lin YAN Hong-meng GAO Lei YILIXIATI•Aireti JIN Hong ZHOU Jian-zhong
    2021, 37(6):201-210. DOI: 10.13982/j.mfst.1673-9078.2021.6.1111
    [Abstract](263) [HTML](373) [PDF 7.87 M](669)
    Abstract:
    The degree of hydrolysis, as measured by potentiometric titration in the presence of formaldehyde, and free radical scavenging rate in five bioenzymes, namely flavor proteases, alkaline proteases, papains, neutral proteases, and pepsins were compared to identify the bioenzyme with the best proteolytic performance against the Prunus dulcis var. amara prolamins under optimal conditions. The effects of independent variables such as enzyme addition, substrate concentration, pH and reaction time were explored via single-factor experiments. Box-Behnken response surface methodology considering these four factors at three levels was then used to optimize the proteolysis of these five bioenzymes. The results demonstrate that alkaline proteases outperform the other enzymes when using the Prunus dulcis var. amara prolamins as substrate. The optimal conditions were determined to be as follows: a reaction time of 4.12 h; a total of 5208.93 U/g of the enzyme should be added; the substrate concentration should not exceed 3.21%; and the pH should be maintained at 9.22. Final evaluations using these conditions allowed for an additional round of refinement and we determined that the optimal conditions are as follows: a reaction time of 4.0 h; 5000.0 U/g of enzyme; substrate at 3.0%; and the pH at 9.0. These conditions were then validated by the evaluation of the end product with these processed petides increasing the DH% and DPPH free radical scavenging rate to 26.74%±0.54% and 97.86%±0.58%, respectively. These values are very similar to the predicted values from our in silico evaluation, confirming that these are the optimal conditions for the proteolysis of the prolamin proteins produced by Prunus dulcis var. amara.
    27  3D Printing Characteristics of Chinese Yam Starch Gel
    WU Jing-nan FENG Lei SONG Jiang-feng LI Da-jing LI Ming ZHANG Zhong-yuan ZHANG Pei-tong LIU Chun-quan
    2021, 37(6):211-216. DOI: 10.13982/j.mfst.1673-9078.2021.6.1086
    [Abstract](296) [HTML](261) [PDF 2.98 M](627)
    Abstract:
    Chinese yam starch was used to explore the effects of starch concentration on rheological properties, 3D printing characteristics, color and texture variations, and microstructures of gel systems. The results showed that, as the shear rate increased, the apparent viscosity of Chinese yam starch gel gradually decreased, indicating that yam starch gel is a typical pseudoplastic fluid system. As the starch concentration increased, the apparent viscosity, storage modulus (G′), and loss modulus (G″) of the gel gradually increased. Hardness, adhesiveness, and chewiness also gradually increased. Under the same conditions, L*, a*, and b* values gradually decreased. Printability, printing accuracy, and stability increased, and the porous network structure of the 3D printed samples displayed a honeycomb-like microstructure. As the porosity decreased, the structure became denser as the starch concentration increased. Sixteen percent starch gel provided the best 3D printability, exhibiting high printing stability with a maximum cohesion of 0.65. The diameter deviation was -2.40% after a cylindrical printed sample had been set aside for 1 h. Height deviation was equal to -1.60%. These findings provide theoretical support for the exploration of raw food materials for 3D printing and full utilization of nutritional functions of Chinese yam starch.
    28  Design and Application of Flat Green Tea Standardized Processing Line
    ZHANG Ming-ming FAN Qi-ye LI Wen-cui WANG Jia-peng TANG Xiao-lin
    2021, 37(6):217-221. DOI: 10.13982/j.mfst.1673-9078.2021.6.1075
    [Abstract](259) [HTML](362) [PDF 1.51 M](672)
    Abstract:
    At present, the standardization level of flat tea processing in China is not high. To solve the problems of middle and high-grade flat tea, such as bar loose and not tight straight enough, as well unstable quality, and so on, according to the processing process and quality forming principle of flat green tea, the key processing equipment of flat green tea was equipped, and a standardized production line was designed. The process parameters of each processing module were optimized according to the specific grade of fresh leaves, and the production line test was carried out. The results showed that, this production line has compact equipment connection, scientific layout, simple operation and stable operation. What’s more, the product had a flat, straight and smooth shape, green and uniform color, green and bright liquor color, high aroma and mellow taste. The leaf bottom bud leaf stretched like flower, with a uniform, green and brightcolor. The quality conformed to the request of flat green tea product. With the increase of production efficiency, the daily production capacity of dry tea could reach 307.20 kg. On the basis of improving the efficiency of medium and high-grade flat green tea, this production line could also guarantee the stability of its quality, which has a good demonstration effect for flat tea production enterprises.
    29  Process Optimization for Ugni Blanc Brandy in Xinjiang Production Area
    SHAO Yong-ming WANG Shu-wei DONG Rong MU Jing-yi LI Gui-lin YAN Xue ZHANG Zhen-zhen
    2021, 37(6):222-230. DOI: 10.13982/j.mfst.1673-9078.2021.6.0978
    [Abstract](286) [HTML](317) [PDF 2.97 M](572)
    Abstract:
    In order to explore the production process of the brandy with Xinjiang characteristics, the traditional brandy brewing and distillation process was applied in this research to the Ugni Blanc grape varieties used for both fresh food and wine in Xinjiang production area. From the aspects of whether clear juice is used for fermentation, sugar content before fermentation, initial pH value and aged oak type, process optimization for the Ugni Blanc brandy in Xinjiang production area was carried out, to obtain the optimal processing conditions: the raw materials are fermented with clear juice is the raw material for fermentation, the initial sugar is adjusted to 20 °brix, the initial pH is 3.5, and the light-roasted oak is used for aging. The sensory score of resulting product was 91.030. After 6 months of natural aging in light-roasted oak, the wine body was in golden yellow color, clear and bright, with a full and harmonious taste and a good style. Through the descriptive aroma analysis, it was found that the characteristic cognac flavor of Ugni Blanc brandy gradually appeared. The safety optimization test was carried out on the Ugni Blanc brandy from the Xinjiang production area, and the final methanol content was 0.580±0.02 g/L it (which fully met the brandy’s methanol safety standard; GB≤2.0 g/L). This research used fuzzy comprehensive evaluation combined with response surface methodology to optimize more accurately, scientifically and objectively the brewing and aging processes of Ugni Blanc brandy from Xinjiang production area, which provides theoretical support for the improvement of the local facultative grape brandy process and the description and analysis of volatile aroma components.
    30  Effect of Extrusion on the Digestibility and Viscosity Properties of Pea Starch
    WANG Meng CHEN Xuan-hong DONG Lei-chao JI Wei NAN Xi-jun ZHOU Quan-cheng
    2021, 37(6):231-236. DOI: 10.13982/j.mfst.1673-9078.2021.6.1035
    [Abstract](214) [HTML](341) [PDF 1.56 M](666)
    Abstract:
    The purpose of this study was to investigate the mechanism of single screw extrusion on the digestion characteristics of pea starch, and to explain the effects of material moisture, screw rotation speed and extrusion temperature. The physical and chemical properties of pea resistant starch were revealed by scanning electron microscope, X-ray diffraction and fast viscosity meter from the perspectives of molecular crystal type, structure and viscosity properties. The results showed that the content of rapidly digestible starch decreased significantly and the contents of resistant starch and slowly digestible starch were affected by extrusion, the highest content of resistant starch was 78.83% under the conditions of water content 26%, screw rotation speed 110 r/min, and extrusion temperature 70 ℃; and the high temperature, high pressure and high shear force during extrusion destroyed the structure of starch granules and transformed them into V crystals. Then the peak viscosity, valley viscosity, final viscosity and setback value of pea resistant starch were 559.41 cP, 474.38 cP, 754.34 cP, 280.33 cP, respectively, which were significantly lower than those of pea starch (p<0.05), and the viscosity curve was smooth. The extrusion could significantly change the digestibility and viscosity properties of pea starch by changing the structure and crystal form of pea starch, which provides new ideas for the modification of pea starch, broadens new fields for high-value starch processing products, provides new materials for the development of related functional products, and also provides a reference for the industrialization process parameters of pea starch.
    31  Quality of Citrus Peels Dried at Different Temperatures using Heat Pump
    YANG Wan-ru YU Yang-yang CHEN Shu-peng YU Yuan-shan FU Man-qin BU Zhi-bin
    2021, 37(6):237-243. DOI: 10.13982/j.mfst.1673-9078.2021.6.0003
    [Abstract](171) [HTML](287) [PDF 933.16 K](704)
    Abstract:
    To understand the effect of drying temperature on the quality of citrus peels dried using a heat pump, the colors, rehydration ratios, essential oil contents, total flavonoid contents, and antioxidant activities of the sun-dried, fresh citrus peels (control) and those dried at different temperatures (40, 50, 60, 70, and 80 ℃) using a heat pump are compared. The L value first increases and then decreases with an increase in the drying temperature. The a values of the citrus peels dried using a heat pump at 40, 50, and 60 ℃ and those of the sun-dried peels show no significant differences (p>0.05). However, the a value decreases with an increase in temperature to >60 ℃. In contrast, the b value increases with an increase in the drying temperature. The lowest ΔE value is obtained when the citrus peels are dried using heat pump at 60 ℃. At equilibrium, the rehydration ratios of sun-dried citrus peels and the peels dried using a heat pump at 40, 50, and 60 ℃ exhibit insignificant differences (p>0.05), but these are significantly higher than those of the peels dried using a heat pump at 70 and 80 ℃ (p<0.05). The essential oil and total flavonoid contents of the peels first increase and then decrease as the drying temperature increases. At 60 ℃, the highest essential oil and total flavonoid contents of 2.04% and 13.46%, respectively, are obtained. The highest DPPH and ABTS+ scavenging capacities of 54.16% and 37.13%, respectively, are also observed at 60 ℃. Therefore, drying using a heat pump at 60 ℃ can allow the preservation of the quality of citrus peels, including the color, total flavonoid content, and antioxidant activity of the peels. This finding can facilitate the practical application of heat pumps in drying citrus peels.
    32  Correlation between Texture Parameters of Jujube Paste and Sensory Quality
    HAO Hai GUAN Shang-wei NIMICEREN•Naqin LIN You-xin LI Huan-rong
    2021, 37(6):244-250. DOI: 10.13982/j.mfst.1673-9078.2021.6.1077
    [Abstract](293) [HTML](361) [PDF 765.93 K](578)
    Abstract:
    In view of the lack of objective evaluation criteria for the quality of jujube paste fillings, the texture profile analysis (TPA) method for jujube paste fillings was established by analyzing the influence of test conditions on TPA parameters of jujube paste fillings. The results showed that the TPA parameters were the best when the probe speed was 5 mm/s, the compression degree of jujube mud surface was 50%, the test residence time was 5 s, and the trigger force of probe to jujube mud stuffing was 5 g. By using the multi-faceted texture analysis method in sensory evaluation, the grading rules of jujube paste filling were formulated. The results of Pearson analysis showed that the roughness of sensory evaluation indexes had different degrees of influence on fragility, hardness and compactness (Pearson coefficient r was between 0.9~1, p<0.01); the correlation analysis of TPA parameters and sensory scores showed that the hardness, adhesion, cohesiveness and chewiness of TPA parameters were significantly correlated with sensory score and resilience at the level of 0.01. Therefore, five TPA parameters of hardness, adhesiveness, cohesiveness, chewiness and resilience were proposed as objective evaluation indexes of filling quality.
    33  Changes and Principal Component Analysis of Flavor Components in Ginkgo Seed before and after Enzymolysis-Fermentation
    YAO Fang ZHAO Yan-sheng WANG Hai-lan LIANG Xiao-xue QI Xing-pu
    2021, 37(6):251-265. DOI: 10.13982/j.mfst.1673-9078.2021.6.0904
    [Abstract](234) [HTML](316) [PDF 2.60 M](604)
    Abstract:
    Headspace solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) technology combined with principal component analysis (PCA) was used to examine the changes of volatile flavor components in Ginkgo seed before and after the enzymolysis-fermentation process. The results showed that the types and relative contents of volatile flavor components of ginkgo seed prepared by different enzymolysis-fermentation processes were significantly different. A total of 212 kinds of volatile flavor components were identified in G, A, B, C, D, and E samples, with 77, 70, 45, 41, 79, and 80 types of flavor components, respectively, being found in these samples. The ginkgo seed produced through the mixed fermentation by amylase along with Lactobacillus plantarum and Saccharomyces cerevisiae (E) had the best flavor, significantly increased types and relative contents of alcohols and esters, and significantly decreased types and relative contents of hydrocarbons and aldehydes. The relative contents of volatile flavor components including nonanal, hexanal, octanal, decanal, hexanal, benzaldehyde, 2,2,4,6,6-pentamethylheptane, decane andxylene, which were the major contributors to the bad smell of ginkgo seed, decreased significantly or were not detected. The pleasant flavor components were produced, such as phenethyl alcohol (25.79%), isoamyl alcohol (15.56%), 2,4,5-trichlorophenylcinnamate (13.29%), 2,3-butanediol (7.10%), 3-methylthiopropanol (3.67%) and lactic acid (3.00%), with their relative content being 68.41%. The six samples were divided into two categories by the principal component analysis, and the comprehensive score of the E sample was the highest, indicating that the E method was the most beneficial method for the formation of good flavor of ginkgo seed. This study provides support for flavor control during the deep processing of ginkgo seed.
    34  Effects of Heating Methods on Quality and Volatile Substance Contents of Frozen Red Bean Paste Buns
    WANG Qiu-yu ZHANG Hai-feng ZHU Wen-zheng XUE Pan-pan SHA Wen-xuan SU Jia-min ZHOU Xiao-yan
    2021, 37(6):266-275. DOI: 10.13982/j.mfst.1673-9078.2021.6.1122
    [Abstract](336) [HTML](247) [PDF 1.43 M](662)
    Abstract:
    The effects of heating methods on the quality and volatile substance contents of frozen red bean paste buns were examined. Frozen red bean paste buns were heated using three methods, namely, steaming using a multifunction oven, steaming using a traditional food steamer, and microwave heating. Changes in quality parameters such as water content, texture, color, specific volume, heating characteristics, and volatile flavor substance contents of the heated red bean paste buns were determined. The results showed that heating methods had significant effects on the quality and relative contents of volatile flavor substances of frozen red bean paste buns (p<0.05). According to water content analysis, microwave heating had the smallest effect in the proton migration rate. Texture analysis revealed that heating using a traditional food steamer yielded the least hard and chewy buns. L* values of the buns heated using microwave were the lowest whereas their a* values were the highest. The specific volume of buns heated using a traditional food steamer was the largest, with the lowest water loss rate. In addition, buns heated using microwave had the highest pH values. Solid phase microextraction and gas chromatography-mass spectrometry assays identified 52, 55, and 54 types of volatile flavor substances from buns heated using the three methods. The identified volatile flavor substances were mainly alcohols, acids, esters, aldehydes, ketones, and phenols. The results further indicated that heating using a traditional food steamer was the most suitable method for frozen dough. These findings shall provide a theoretical basis for the selection and control of heating methods for frozen dough.
    35  Evaluation for Lactic Acid Bacteria Safety in Traditional Fermented Food
    WAN Qian LI Qi-ming WU Hua-xing LIU Rong-mei CAO Jun HUANG Xiao-ying YU Ji-cheng TANG Jun-ni
    2021, 37(6):276-286. DOI: 10.13982/j.mfst.1673-9078.2021.6.1057
    [Abstract](342) [HTML](369) [PDF 1.27 M](634)
    Abstract:
    The 38 lactic acid bacteria (LAB) isolated from traditional fermented foods were used as the research object. The hemolysis test, gelatin liquefaction test, and indole test were used to test the toxin-producing metabolites of the tested strains. The K-B method was used to detect the sensitivity of strains to 25 kinds of antibiotics, and polymerase chain reaction was used to detect the drug resistance genes carried by the strains. The results showed that the hemolysis test, gelatin liquefaction test and indole test of 38 strains of lactic acid bacteria were all negative, indicating that the strains did not produce related toxic substances in the process of growth and metabolism. Thirty-eight strains of lactic acid bacteria were all sensitive to 7 antibiotics including ceftriaxone, cefotaxime, tetracycline, doxycycline, chloramphenicol, clindamycin, erythromycin; some strains were resistant to the other 18 antibiotics to varying degrees, with the highest resistance rate to sulfisoxazole (52.63%), followed by kanamycin (34.21%), vancomycin (31.58%), amikacin (26.32%), streptomycin (15.79%), rifampicin (2.63%). The drug resistance spectrum analysis showed that the tested Lactobacillus plantarum had multiple resistance phenomenon. PCR detected 3 kinds of resistance genes, namely chloramphenicol resistance gene catA (2.63%), vancomycin resistance gene vanA (5.26%), rifampicin resistance gene rpoB (42.11%). The results showed that 38 strains of lactic acid bacteria isolated from traditional fermented foods had no safety risks related to toxin-producing metabolites, but they were resistant to some antibiotics, and the detection rate of rpoB was high. This study provides a theoretical reference for the improvement of lactic acid bacteria safety in traditional fermented food.
    36  Analysis of Metabolites of Soybeans from Different Producing Origins in Heilongjiang Province Based on Metabonomics
    ZHANG Li-yuan DAI An-na YU Run-zhong RUAN Chang-qing LI Zhi-jiang ZHANG Dong-jie
    2021, 37(6):287-295. DOI: 10.13982/j.mfst.1673-9078.2021.6.1032
    [Abstract](245) [HTML](251) [PDF 2.26 M](666)
    Abstract:
    Metabolites of Heihe 43 soybeans in three origins (Beian Longmen, Weishan and Yinlong River) were isolated and identified using non-targeted metabolic profiling based on gas chromatography-mass spectrometry. The metabolites in the soybean samples were extracted by 80% methanol, derivatized with N,O-bis (trimethylsilyl) trifluoroacetamide, eluted on an HP-5ms column, detected with GC-MS. The metabolic pathways of the differential metabolites were analyzed by KEGG annotation, the metabolic mechanisms were explored. A total of 68 metabolites were detected in soybean samples, and 62 metabolites were isolated and identified, included 22 carbohydrates and their derivatives, 14 fatty acids and their derivatives, 7 alcohols, 4 esters, and 2 amino acids, and 13 kinds of intermediates, inferring the structure of 6 kinds of unknown metabolites, 4 kinds of WS-soy samples, 2 kinds of YLH-soy samples. Soybean metabolites and metabolic mechanisms were different in different producing areas, and they had the attributes of producing areas. This not only provides a theoretical basis for the quality analysis of soybeans in cold regions, and also provides a basis for soybean classification and processing or extraction of functional components by origin.
    37  High-resolution Melting Curve Analysis Coupled with Fluorescence Real-time PCR for Simultaneous Detection of Four Food-borne Pathogenic Bacteria in Chicken
    ZHANG Jing ZHOU Qian TANG Meng-jun ZHANG Xiao-yan TANG Xiu-jun LU Jun-xian GAO Yu-shi GU Rong
    2021, 37(6):296-303. DOI: 10.13982/j.mfst.1673-9078.2021.6.0908
    [Abstract](168) [HTML](355) [PDF 1.68 M](661)
    Abstract:
    A high-resolution melting curve analysis (HRM) technique was established for simultaneous and rapid detection of Salmonella, Listeria monocytogene, Campylobacter Staphylococcus aureus and Campylobacter jejuni in chicken meat. In this study, the fimY, hly, nuc and orfC specific genes of the above four bacteria were used as target genes to establish amultiple HRM-real time PCR detection system that can simultaneously detect a variety of food-borne pathogens. The specificity, sensitivity and reproducibility of the reaction and the artificially infected samples were evaluated. The established HRM-real time PCR detection system produced specific melting curves for Salmonella, Listeria monocytogene, Staphylococcus aureus and Campylobacter jejuni, with the TM values being 82.2 ℃, 78.2 ℃, 75.5 ℃ and 73.5 ℃, respectively. The sensitivity of the multiple HRM-real time PCR reaction system was 102 copies/mL for single pathogenic bacterium solution and 102 copies/mL for multiple pathogenic bacteria solution. The intra-CV value in the repeatability test of the reaction system was good between 0.03% and 0.32%, and the inter-CV value was between 0.45% and 2.12. The detection limits of the four bacteria for the artificially infected samples all reached 5 cfu/25 g. The results showed that the multiple HRM real-time PCR system can effectively detect and distinguish the above four common food-borne pathogens in poultry meat. The method has high specificity and sensitivity and good response repeatability, thus, is expected to play a role in the detection of food-borne pathogens.
    38  Analyze Nutritional Components of Bambusa emeiensisi Shoots and Antioxidant Activity of Its Extracts based on Widely Targeted Metabolomics
    TANG Hao LI Yuan-qiu GAN Xiao-feng LUO Chao-bing
    2021, 37(6):304-311. DOI: 10.13982/j.mfst.1673-9078.2021.6.1007
    [Abstract](278) [HTML](294) [PDF 1.71 M](605)
    Abstract:
    In the present study, the nutritional components of Bambusa emeiensisi shoots were analyzed by gas chromatography-mass spectrometry (GC-MS) and liquid chromatography tandem mass spectrometry (LC-MS/MS), and the antioxidant activity was systematically investigated by elimination of DPPH?, O2-?and OH?, reducing power and total oxidation resistance. The results showed that a total of 75 metabolites were identified in GC-MS metabolome, among which the main metabolites with more than 10% content were carbohydrate, such as glucose (34.98%), fructose (19.18%) and sucrose (14.99%), and the relative contents of another 9 metabolites were more than 1%. A total of 226 metabolites were detected in LC-MS/MS metabolome, the highest relative content of which was organic acids. The relative content of 16 metabolites was greater than 0.5%, and there were 9 metabolites whose relative content was greater than 1%. The antioxidant activity results showed that the content of the extracts was positively correlated with the free radical scavenging capacity, relative reducing capacity and total antioxidant capacity, among which the DPPH scavenging rate of the 1 mg/mL extract was 82.70%, the hydroxyl radical scavenging rate was 89.70%, the superoxide radical scavenging rate was 43.80%, the relative reducing power was 27.90%, and the FRAP value was 100.10 mol/L. B. emeiensisi shoots are rich in sugars, organic acids, amino acids and other components and exhibit high antioxidant activity, so they have a great prospect in the processing of functional food.
    39  Determination of Migration of Chloropropanols from Food Contact Paper Products by GC-MS
    ZENG Ying CHEN Yan-fen HUANG Qiao WU Ze-chun LI Dan ZHONG Huai-ning MO Ce-hui
    2021, 37(6):312-319. DOI: 10.13982/j.mfst.1673-9078.2021.6.0996
    [Abstract](431) [HTML](322) [PDF 865.10 K](696)
    Abstract:
    A method for simultaneous determination of 1,3-dichloro-2-propanol (1,3-DCP), 2,3-dichloro-1-propanol (2,3-DCP), 2-chloro-1,3-propanol (2-MCPD) and 3-chloro-1,2-propanol (3-MCPD) from food contact paper products was established by using gas chromatography-mass spectrometry (GC-MS). 4% acetic acid, 10% ethanol, 50% ethanol and olive oil were selected as food simulants according to GB standard. The method was optimized in this study, the limit of detection (LOD) and limit of quantitation (LOQ) for each chloropropanols were 2 μg/kg and 10 μg/kg, respectively. The spiked recoveries were 87.3%~109%, and the relative standard deviations were 1.1% ~ 9.5% (n=6). The method is demonstrated to be suitable for determination of migration of chloropropanols from food contact paper with well accuracy and reproducibility. The method was applied to detection of 15 food contact papers, the highest value for both detection frequency and migration was observed for 3-MCPD. The detection frequency of 1,3-DCP and 3-MCPD were 33.3% and 53.3%, respectively ,and the migration level was 2.27~12.04 μg/kg and 2.51~183.32 μg/kg, respectively, while both migration of 2,3-DCP and 2-MCPD were not detected. It was also found that the migration of chloropropanols in the aqueous simulants was higher than that in olive oil. In view of the high detection frequency of 1,3-DCP and 3-MCPD, more attention should be paid to the risk caused by the migration of chloropropane in food contact paper.
    40  Using Rice Soaking and Rinsing to Remove Pseudomonas cocovenenans subsp. farinofermentans during Wet Flour Production
    CHEN Han-jin CHEN Rong-qiao ZHU Wen-xin XIAN Yan-ping HOU Xiang-chang WU Yu-luan HU Jun-peng LIU Dong-hong DAI Hang
    2021, 37(6):320-325. DOI: 10.13982/j.mfst.1673-9078.2021.6.0128
    [Abstract](198) [HTML](415) [PDF 2.66 M](625)
    Abstract:
    One of the issues facing wet flour (products made up of wet rice and starch flour) production is the removal of contaminating microorganisms. This study was designed to evaluate the effectiveness of soaking and then rinsing the rice to remove Pseudomonas cocovenenans subsp. farinofermentans during the production of wet flour. First all of the contamination models were constructed by culturing Pseudomonas cocovenenans subsp. farinofermentans at 36 ℃ for 72h to produce biofilms on the surface of the rice samples. Then these samples were processed using the soaking and rinsing techniques (static immersion cleaning as well as slow dynamic stirring cleaning) commonly applied in current wet flour production protocols. Microbial detection, animal toxicity tests and LC/MS-MS were combined to examine the effectiveness of rice soaking and rinsing on inhibiting the spread of Pseudomonas cocovenenans subsp. farinofermentans contamination. The results demonstrate that both static immersion and slow dynamic stirring cleaning can eliminate some Pseudomonas cocovenenans subsp. farinofermentans, but total removal is not guaranteed. It is possible that contamination may still be present and passed to the next steps in the processing workflow. Thus, it is recommended that the soaking and rinsing of the raw materials, i.e. rice, should be closely monitored and strictly enforced during wet flour production. At the same time, production sites should be thoroughly cleaned and disinfected after each shift. In this way, the risks of Pseudomonas cocovenenans subsp. farinofermentans contamination may be effectively controlled.
    41  Preparation and Application of ELISA Kitfor Detection of Sunset Yellow
    XIE Qian LI Qing LI Jia-nan
    2021, 37(6):326-332. DOI: 10.13982/j.mfst.1673-9078.2021.6.0947
    [Abstract](226) [HTML](282) [PDF 1.74 M](558)
    Abstract:
    In order to detect quickly and accurately sunset yellow in food, the sunset yellow was coupled with a carrier protein by the carbonyl diimidazole method and ultraviolet spectroscopy was used for identification of sunset yellow in this study. The results showed that the antigen and immune antigen for sunset yellow detection were successfully synthesized. A monoclonal antibody with a titer of 1:64000 was prepared by immunizing mice and an ELISA kit was developed for detecting sunset yellow. The results of the kit’s detection and analysis performance showed that the linear detection range of the kit’s standard curve was from 62.50~1000 ng/mL with the detection limit being 1.50 ng/mL and the detection sensitivity IC50 value as 6.50 ng/mL; the cross-reaction rate of the monoclonal antibody towards sunset yellow in this kit was 100%. A weak cross-reaction towards allure red (reaction rate: 2%) was found, with no cross-reactions towards other two color additives were detected. The intra-assay recoveries and inter-assay recoveries of the commercially available samples such as fruit juices, canned fruits and jellies were in the ranges of 89.18%~101.13% and 87.69%~97.90%, respectively, with the relative standard deviations of the intra-assay and inter-assay tests less than 10%. These results demonstrated that the ELISA kit was sensitive, accurate, convenient and fast. In this study, an immunological method was used to prepare successfully an ELISA kit for sunset yellow detection, and the sunset yellow added in fruit products was detected, which provides an efficient and accurate experimental method for large-volume detection of the sunset yellow content in food in large quantities.
    42  Advances on Detection Techniques for Foodborne Listeria monocytogenes
    LIU Hui ZENG Xiang-quan XIE Wen-dong JIANG Shi-wei DU Hong-miao ZHOU Yu-chun HUANG Hua
    2021, 37(6):333-344. DOI: 10.13982/j.mfst.1673-9078.2021.6.1079
    [Abstract](275) [HTML](310) [PDF 2.16 M](691)
    Abstract:
    Listeriosis caused by Listeria monocytogenesis currently considered to be one of the leading foodborne diseases worldwide, with a mortality rate of 20~30%. L. monocytogenes can be found in most foods and proliferate in a wide range of pH and osmolarity, at low temperatures, and under aerobic and anaerobic conditions. Thus, effective monitoring and control over L. monocytogenes along the food chain are necessary to prevent food contamination and outbreak of foodborne diseases. Foodborne L. monocytogenes is a low-cell-count pathogenic bacterium similar to other non-pathogenic Listeria regarding colony morphology and physicochemical characteristics, which makes its detection challenging. This study comprehensively reviewed the traditional culture methods and emerging alternative protocols, such as immunoassays, molecular bioassays, biosensors, bacteriophage-based methods, and compared their respective advantages and disadvantages, so as to provide a reference for rapid detection of L. monocytogenes in food.

    Editor in chief:李琳

    Inauguration:现代食品科技

    International standard number:ISSN 1673-9078

    Unified domestic issue:CN 44-1620/TS

    Domestic postal code:46-349

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