Volume 37,Issue 3,2021 Table of Contents

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  • 1  Improvement of Rheumatoid Arthritis with Lactobacillus plantarum HG20 in Rats
    LIU Chun-hong LIN Li XIAO Ru-xue CUI Wei-dong ZHAO Zi-jian LI Sheng-yu
    2021, 37(3):1-6. DOI: 10.13982/j.mfst.1673-9078.2021.3.0711
    [Abstract](441) [HTML](434) [PDF 2.03 M](968)
    Abstract:
    In order to obtain a new strain of lactic acid bacteria with adjuvant therapy of rheumatoid arthritis, morphological observation, API50 CHL sugar fermentation experiment, 16S rDNA and pheS gene sequence comparison were performed to isolate a strain HG20 from the surface of cucumber for further identification. The therapeutic effect of the HG20 strain on rheumatoid arthritis model rats induced by type II collagen was evaluated according to the indices including the degree of ankle swelling, levels of serum inflammatory factor, and rheumatoid-related indicators. The results showed that HG20 was identified as a Lactobacillus plantarum strain. Animal experiment results showed that after 21 days of administration, the ankle circumference of rats in the HG20 group decreased from 3.98 cm to 1.31 cm (by 65.52%), and the toe volume decreased from 6.61 mL to 2.35 mL (by 61.28%).Compared with the model group, L. plantarum HG20 could significantly reduce the levels of serum inflammatory cytokines (IL-1β by 38.26%; TNF-α by 52.58%), increase the expression of anti-inflammatory factor (IL-10 by 55.38%),reduce C-response protein (CRP by 12.89%) and immunoglobulin (IgG by 46.07%; IgM by 34.29%). In summary, L. plantarum HG20 has adjuvant therapeutic effects on rheumatoid arthritis through reducing inflammatory response and regulating autoimmunity. It is a new probiotic strain with potential application prospects.
    2  Meta-analysis of the Mechanism of Walnut Kernel against Type 2 Diabetes
    ZHAO Sheng-lan ZHANG Feng ZHANG Li-ming GUO Yan CHEN Chao-yin
    2021, 37(3):7-15. DOI: 10.13982/j.mfst.1673-9078.2021.3.0198
    [Abstract](363) [HTML](415) [PDF 36.63 M](975)
    Abstract:
    The intervention mechanism of walnut kernel on type 2 diabetes was investigated by meta-analysis in this study. TCMSP database was used to screen the main active ingredients and potential targets of walnut kernels. Targets for type 2 diabetes-related diseases were searched in the OMIM, TTD and genecards databases, and the active component targets and disease targets were intersected using R3.6.2 software. A network diagram was constructed using cystoscope 3.7.2 software. The analyses of protein interaction, GO function and KEGG pathway enrichment were performed on the intersection targets through STRING. As a result, 83 targets of 11 active ingredients, such as gallic acid, gallic tannins and pentagalloylglucose were obtained, which were intersected with 3015 targets related to type 2 diabetes to obtain 28 targets of walnut component for type 2 diabetes intervention, mainly including dioxygenase 2, coagulation factor 2, and catalase, IL5 and glucose-6-phosphate 1-dehydrogenase. These targets involved GO functions such as hormone metabolism, cellular lipid metabolism, fatty acid biosynthesis and cholesterol metabolism, and in vitro and in vivo responses to organic and inorganic substances, which participated mainly in four categories of disorders, diabetes, cancer, circulatory system and inflammation, along with 17related pathways, as well as KEGG pathways such as endocrine, signal transduction, thyroid and neurotransmitter. The results showed that walnut kernels may participate in lipid metabolism regulated by signaling pathways such as MAPK, AGE-RAGE and PI3K-Akt signaling pathways with multiple targets such as PRKD1, PRKCA,PRKCB, PRKCE, PRKCZ, IL5, F2, CAT, and G6PD, to achieve type 2 diabetes intervention effects.
    3  Inhibition Effects of Loganin on Fetal Bovine Serum-induced Steatosis in Human Liver Cell Line L02
    LIU Shu-min SUN Jia-wei CHEN Xiao-dan PAN Yi WANG Biao-shi
    2021, 37(3):16-22. DOI: 10.13982/j.mfst.1673-9078.2021.3.0819
    [Abstract](329) [HTML](405) [PDF 12.26 M](1118)
    Abstract:
    In this research, 50% fetal bovine serum (FBS)-induced steatosis in human liver cell line L02 cell was used as model to investigate the therapeutic effects of loganin on cellular steatosis. The inhibitive effect of loganin on cell proliferation was detected with MTT method and Oil red O staining method. The enzymatic activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) and levels of total cholesterol (TC) and triglycerides (TG) in cell supernatant, as well as the intracellular levels of TC, TG and thiobarbituric acid reactive substances (TBARS) were determined with corresponding assay kits. MTT results showed that compared with the model group, different concentrations of loganin could significantly inhibit the growth of steatosis L02 cells (p<0.05), the inhibitory rate was 14.6%~26.91%. Oil red O staining showed that the accumulation of hepatic lipid in the loganin treatment groups was significantly decreased with the increase in concentration. Compared with the model group, the extracellular enzyme activities of AST, ALT and LDH in loganin treatment groups were reduced by 26.70%~52.58%, 24.83%~61.23% and 14.53%~28.83%, respectively; extracellular levels of TC and TG were reduced by 50.46%~68.76% and 32.51%~53.37%, respectively; intracellular levels of TC and TG were reduced by 11.48%~32.79% and 10.50%~18.30%, respectively; intracellular levels of TBARS were reduced by 40.33%~60.10%. These inhibitory effect of loganin treatment groups on cellular steatosis was dose-dependent and better than that of spontaneous recovery group. The results indicated that loganin could inhibit FBS-induced cellular steatosis in L02 cells via effectively reducing lipid metabolic disorders and lipid peroxidation in L02 cells.
    4  Improving the Immunity and Intestinal Tract of Macrobrachium rosenbergii by Enterococcus durans 6-24 and Bacillus subtilis B9
    ZHU Cheng-kun QIN Zhen-ning HAN Shuang-yan LIANG Shu-li LIN Ying
    2021, 37(3):23-31. DOI: 10.13982/j.mfst.1673-9078.2021.3.0302
    [Abstract](333) [HTML](431) [PDF 17.22 M](743)
    Abstract:
    Enterococcus durans 6-24 and Bacillus subtilis B9 were isolated from the intestine of shrimp through antibacterial tests, adhesion tests and specific hydrolase activity determinations, in combination with 16S rDNA and physiological and biochemical identification. A preliminary study was carried out on the probiotic effects of 6-24 and B9 in feeding Macrobrachium rosenbergii. After the two strains were added to the feed at 1×107 cfu/kg, a 4-weekfeeding experiment started, and the changes in total blood cells, prophenoloxidase system (proPO), antioxidant system and lysozyme of M. rosenbergii were determined. The research results showed that after M. Rosenbergii was fed with the feed containing 6-24 and B9, the total hymphocyte count increased significantly (p<0.05), with the activities of phenoloxidase, superoxide dismutase and lysozyme increased simultaneously (phenol oxidase increased by 48.24%; lysozyme increased by 198.00%). The experiments on the intestinal digestive enzymes of Macrobrachium rosenbergii revealed that compared to the control, the activities of digestive enzymes (amylase, protease, lipase) of the experimental group fed with mixed probiotics increased significantly (p<0.05) (among which, the protease increased by 84.80%). Finally, the research revealed that the number of cultivable Vibrio in the intestine of Macrobrachium rosenbergii fedwith6-24 and B9 decreased by 59.76% (p<0.05). The results showed that Enterococcus durans 6-24 and Bacillus subtilis B9 can improve the intestine environment and immunity level of M. rosenbergii, and have the potential to become probiotic shrimp feed additives.
    5  Astragaloside IV Inhibits Proliferation and Induces Apoptosis of Melanoma B16 Cells
    WANG Ri-ming NING Xue REN Yue-ying
    2021, 37(3):32-36. DOI: 10.13982/j.mfst.1673-9078.2021.3.0860
    [Abstract](273) [HTML](581) [PDF 631.75 K](715)
    Abstract:
    The effect of astragaloside IV on the proliferation and apoptosis of melanoma B16 cells was investigated. After the cells were cultured successfully, they were divided into blank control group, low concentration group (10 mg/mL), medium concentration group (20 mg/mL) and high concentration group (50 mg/mL). The effects of astragaloside IV on proliferation and apoptosis of melanoma B16 cells were evaluated, and the expression levels of caspase-3, B-cell lymphoma-2 (Bcl-2), bax and β-catenin were detected. The results showed that astragaloside IV had good antioxidant activity. When treated with 50 mg/mL, the reducing ability and DPPH free radical scavenging capacity were 0.92 and 70.82%, respectively. Under this concentration, the proliferation rate and apoptosis rate of B16 cells after 72 h treatment were 15.55% and 40.45%, respectively, which were significantly different from those of other groups (p<0.05). In addition, the expression levels of caspase-3, Bcl-2, bax and β-catenin were 0.56, 0.58, 0.27 and 0.25, respectively, which were significantly different from those of other groups (p<0.05). Results indicated that astragaloside IV could inhibit the proliferation of melanoma B16 cells and induce apoptosis by regulating the expression of caspase-3, Bcl-2, bax and β-catenin.
    6  Effect of Zanthoxylum Alkylamides on the Disorder of Glucose and Lipid Metabolism in Type 2 Diabetic Mice
    LU Min-tao LUO Jing REN Ting-yuan
    2021, 37(3):37-45. DOI: 10.13982/j.mfst.1673-9078.2021.3.0771
    [Abstract](334) [HTML](419) [PDF 4.65 M](1198)
    Abstract:
    To study the therapeutic effect of Zanthoxylum on glucose and lipid metabolism disorder in type 2 diabetes mellitus (T2DM) mice, the mouse model of T2DM was established by using high fat and high sugar feed and streptozotocin (STZ). The mice were divided into blank group, model group, high dose of Zanthoxydim (0.8 mg/kg bw), low dose of Zanthoxydim (0.4 mg/kg bw) and positive control (100 mg/kg bw) groups, with 10 mice in each group. The feed intake, water intake, body weight and fasting blood glucose of mice were measured during the experiment. Glucose tolerance (total cholesterol, TC), triglyceride (TG), high-density lipoprotein (HDL-C), low-density lipoprotein (LDL-C) and malondialdehyde (MDA) in serum and liver of mice were measured, and the morphological changes of mouse liver tissues in each group was observed by HE staining after 28 days of intragastric administration. The results showed that Zanthoxylum can significantly (p<0.05) reduce the contents of TG, TC, LDL-C and MDA in serum, and significantly (p<0.05) increase the content of HDL-C; compared with the model group, the contents of TG, TC and MDA in the liver of the high-dose group decreased significantly (p<0.05) by 28.31%, 43.56% and 52.14%, respectively, which were equivalent to those of the positive control group. And the morphological test results showed that after 28 days of treatment, compared with the model group, the degree of liver tissue damage in the high and low dose groups was significantly reduced. The results indicated that Zanthoxylum can improve glucose and lipid metabolism and stress response in T2DM mice.
    7  Okra Polysaccharides Improve Glucose and Lipid Metabolism and Kidney Damage in Diabetic Rats
    ZHANG Yan-jun LI Jing ZHANG Yu-ling HAN Yong-hong CHEN Er-hua
    2021, 37(3):46-52. DOI: 10.13982/j.mfst.1673-9078.2021.3.0446
    [Abstract](400) [HTML](331) [PDF 5.30 M](781)
    Abstract:
    The effects of okra polysaccharides on glucose and lipid metabolism and renal pathological changes in diabetic rats were investigated in the present study. The rat model of diabetes was constructed by intra peritoneal injection of streptozotocin. The successfully modeled diabetic rats were randomly divided into a model group, metformin group, and low-, medium- and high-dose okra polysaccharide groups. The okra polysaccharides were administered continuously for 28 days, the levels of blood glucose and serum lipids as well as renal pathological changes in all groups of rats were determined, while the effect of okra polysaccharides on the activity of α-glycosidase was measured by the pNPG method. The results showed that compared with the model group, the levels of fasting blood glucose (FBG) and glycosylated hemoglobin (HbA1c) of the okra polysaccharides-treated groups decreased, with the greatest reduction occurring to the high-dose group (decreased by 32.80% and 17.87%, respectively). In terms of blood lipids, compared with the model group, the total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL) of the okra polysaccharides-treated groups decreased, with the high-dose group having the greatest decrease (by 21.55%, 38.98%, 40.70%, respectively). The high density lipoprotein (HDL) contents increased for all groups, with the highest increase of 47.46% in the high-dose group.In the meantime, the okra polysaccharides could significantly inhibit the activity of α-glucosidase, with the inhibition rate increasing with an elevated dose. When the polysaccharide concentration was 10 mg/mL, the inhibition rate was 68.26%. The okra polysaccharides can alleviate glomerular atrophy and renal tubule swelling in diabetic rats. Therefore, okra polysaccharides can improve blood glucose and lipid levels, and can improve renal pathological changes in diabetic rats. Okra polysaccharides have broad prospects in the development of hypoglycemic dietary supplements, health foods and hypoglycemic drugs. Okra polysaccharide can improve the blood sugar and blood lipid level of diabetic rats, and improve the pathological changes of the kidney tissue of diabetic rats. It has broad prospects in the development of hypoglycemic dietary supplements, health foods and hypoglycemic drugs.
    8  (His)6-tag’s Location and N/C-terminal Truncation Changed the Enzymatic Characteristics of Vibrio parahaemolyticus Phospholipase D
    WANG Fang-hua LIU Si-yu WEI Rui-xia YANG Bo WANG Yong-hua
    2021, 37(3):53-61. DOI: 10.13982/j.mfst.1673-9078.2021.3.0401
    [Abstract](227) [HTML](288) [PDF 32.21 M](990)
    Abstract:
    In the present study, the effects of (His)6-tag’s location and N/C-terminal truncation on the enzymatic characteristics of recombinant Vibrio parahaemolyticus phospholipase D (VpPLD) were investigated. It was found that (His)6-tag’s location had a great influence on the activity of VpPLD. When (His)6-tag was located at the N-terminal, VpPLD exhibited the highest activity. The lowest VpPLD activity was detected when (His)6-tag was located at the C-terminal of VpPLD, with the differences in activity independent on the hydrolysis substrate. In addition, the optimum pH of VpPLD was changed from 7.0 to 8.0 due to the location of (His)6-tag at its C-terminal. When the first 34 amino acids at the N-terminus of mature VpPLD were deleted, the hydrolytic activity of VpPLD towards 1,2-Dimyristoyl-sn-glycero-3-phospho-L- serine (DMPS) was significantly enhanced, although no change was found in the hydrolytic activity towards other phospholipids. When the C-terminal amino acids (469~487) of VpPLD were deleted, its catalytic activities towards all the tested phospholipids decreased significantly. Compared with (His)6-VpPLD-WT and (His)6-VpPLD-Δ469-487, the selectivity of (His)6-VpPLD-Δ451-487 for1,2-Dipalmitoyl-sn-glycero-3- phosphocholine (DPPC), 1,2-Dimyristoyl-sn-glycero-3-phospho-(l’-rac-glycerol) (DMPG) and 1,2-Dimyristoyl-sn-glycero-3- phosphoethanolamine (DMPE) decreased significantly, indicating that the C-terminal amino acids (451~469) had a great impact on the substrate selectivity of VpPLD. The above results showed that the position of (His)6-tag and the N/C-terminal peptide had an important effect on the catalytic activity and substrate selectivity of the VpPLD.
    9  Variations and Correlation between Bacterial Communities in Daqu and the Workshop Air at Different Temperatures
    YAO Ya-lin DENG Jie REN Zhi-qiang WEI Chun-hui ZHONG Shu-xia HUANG Zhi-guo
    2021, 37(3):62-69. DOI: 10.13982/j.mfst.1673-9078.2021.3.0774
    [Abstract](356) [HTML](289) [PDF 27.35 M](1371)
    Abstract:
    The high-throughput sequencing technology was used to study the bacterial community structure of Daqu (the medium-temperature Daqu and the high-temperature Daqu) and the workshop air, and explore the correlation between Daqu and air bacterial communities; at the same time, combined with environmental factors and the composition of Daqu bacterial communities, preliminary verification of environmental factors on Daqu microbial community relevance was performed. The results revealed that 33 bacterial genera were obtained from medium temperature Daqu and air, and the number of bacterial species showed an opposite trend to the change of fermentation temperature; 31 bacterial genera were obtained from high temperature Daqu and air, and the number of bacterial species showed a decreasing trend throughout the fermentation process. Principal component analysis showed that the composition of air bacterial communities in Daqu and workshop air at middle-temperature was basically similar in the early stage of fermentation (except the second stage in fermentation), the composition of the high-temperature Daqu bacterial community was similar throughout the fermentation process, and was similar to that of the workshop air bacterial community in the early fermentation stage. Redundant analysis showed that the 6 factors, including starch content, moisture, acidity, humidity, room temperature, and Daqu temperature, explained 57.41% of the species distribution of Daqu community. The Monte Carlo substitution test found that starch content, moisture and acidity are the main factors affecting the composition of Daqu bacterial community. The dynamic change rule and correlation of bacterial community between Daqu and air during Daqu fermentation were revealed, which provided an important reference for Daqu production and research in the future.
    10  Preparation of Low-alcohol Pineapple Wine by Different Methods of Terminating Fermentation
    WANG Yan ZENG Xin-an CAI Jin-lin
    2021, 37(3):70-76. DOI: 10.13982/j.mfst.1673-9078.2021.3.0297
    [Abstract](460) [HTML](280) [PDF 16.84 M](777)
    Abstract:
    In this study, the processes of semi-fermented pineapple wine involving different methods of terminating fermentation (heat treatment, sulfur dioxide addition and pulsed electric field sterilization) and the quality of derived products were compared. The results showed that the three methods could allterminate effectively the fermentation: using treatment time 80 μs and field strength 15 kV/cm for the pulsed electric field treatment, using water bath at 75 ℃ over 20 min for heat treatment, or using sulfur dioxide at 150 mg/L for the sulfur dioxide addition method. Different methods of terminating fermentation affected mainly the color, aroma and other sensory attributes of fruit wines, but had insignificant effects on the basic physical and chemical indicators such as alcohol content, total acid, and dry extract of low-alcohol wine. The characteristic aroma components of low-alcohol pineapple wine were 2-methylbutyrate (4.71 mg/L), ethyl butyrate (1.20 mg/L), ethyl 2-methyl butyrate (0.58 mg/L), 4-hydroxy-2,5-dimethyl-3(2H)-furanone (0.46 mg/L) and 3-hydroxyhexanoate methyl ester (0.36 mg/L), which were all higher than those of the fruit wine prepared via the traditional method of terminating fermentation. Comprehensive analyses combined with sensory evaluation revealed that the low-alcohol pineapple wine prepared via terminating fermentation by pulse electric field was superior to that prepared by the traditional method of terminating fermentation.
    11  Synthesis of the Furfural Residue Composite for Immobilized Pectinase
    BI Shu-xian HAO Hong-ying WANG Ming-yuan ZHAN Hai-juan LIU Wan-yi
    2021, 37(3):77-82. DOI: 10.13982/j.mfst.1673-9078.2021.3.0802
    [Abstract](228) [HTML](253) [PDF 3.83 M](637)
    Abstract:
    In this paper, furfural residue was used as raw material for the sulfonated pretreatment. The furfural residue-chitosan-glutaraldehyde composite material and the sulfonated furfural residue-chitosan-glutaraldehyde composite material were obtained by wrapping chitosan and crosslinking with glutaraldehyde, using electrostatic self-assembly; the appearance and properties of the two new kinds of materials were characterized by FT-IR and SEM. Then, they were used for immobilizing pectinase, and the catalytic performance and stability of the novel immobilized enzyme were optimized by single factor variable method. The optimum catalytic conditions for the immobilized enzyme of uncarbonized furfural residue composite material were determined as: pH=3.5, pectinase concentration = 50 mg/mL, pectin concentration = 15 mg/mL, reaction time = 120 min, reaction temperature = 45 ℃. The optimum catalytic conditions for the immobilized enzyme of carbonized furfural residue composite material were as below: pH = 3.5, pectinase concentration = 20 mg/mL, pectin concentration = 10 mg/mL, reaction time = 60 min, reaction temperature = 50 ℃. The relative enzyme activities of the two immobilized enzymes were 95.52% and 95.03%, respectively. Under the optimal reaction conditions, the maximum enzyme loading of the two immobilized enzymes were 197.20 mg/g and 60.40 mg/g, respectively. The residual relative activity of immobilized pectinase in furfural residue composite was 81.78% after 8 times of recycling, and the immobilized pectinase activity of sulfonated furfural residue composite was still 88.98% after 32 days of storage at 4 ℃. The two kinds of immobilized enzymes show better loading enzyme capacity and storage stability, and have better economic value and application prospects.
    12  Cloning and Bioinformatics Analysis of Arginine Decarboxylase Gene in Scomberomorus niphonius
    HA Si ZHOU Jia-min HAN Ling-yu ZOU Yu MA Kun LI Ting-ting
    2021, 37(3):83-88. DOI: 10.13982/j.mfst.1673-9078.2021.3.0686
    [Abstract](301) [HTML](260) [PDF 27.16 M](870)
    Abstract:
    In this study, Enterobacter hormaechei, a spoilage amine-producing bacteria during storage of mackerel, was used as the research object. The physico-chemical properties of arginine decarboxylase of Enterobacter hormaechei in Scomberomorus niphonius were examined by various of genetic engineering and bioinformatic methods. The ADC gene was cloned and sequenced by T-A, translated into a protein sequence, before the primary structure, secondary structure and three-dimensional conformation of the protein were predicted, and the physico-chemical properties and functions of the ADC protein were analyzed. The results showed that the ADC protein was composed of 93 amino acids, with the estimated molecular weight roughly as 10.82 ku, the theoretical isoelectric point as 8.64, the atomic composition as C492 H746 N136 O135 S3, and the half-life >10 h (E. coli, in vivo), and the fat coefficient as 86.02. With the total average hydrophilicity as -0.35, the overall performance of the protein was hydrophilic. Behaving like a soluble protein with the instability coefficient as 48.57, the ADC protein did not have a signal peptide and was a secreted protein. These results as well as the simultaneously predicted secondary structure and three-dimensional conformation of the ADC protein provide a reference for subsequent in-depth research. In the future, on the basis of gene cloning, protein expression, separation and purification and properties research should be carried out to analyze further arginine decarboxylase.
    13  Analysis of Daqu Enzymes from the Core Production Area of Maotai-flavor Liquor
    YIN Li QIU Shu-yi CAO Wen-tao BAN Shi-dong WANG Xiao-dan
    2021, 37(3):89-96. DOI: 10.13982/j.mfst.1673-9078.2021.3.0749
    [Abstract](468) [HTML](298) [PDF 27.60 M](979)
    Abstract:
    In this study, Daqu from the Maotai-flavor liquor core production area of Maotai Town and the molds isolated from the Daqu were used as research materials, the physicochemical indices, hydrolase activity, fungal hydrolase composition and enzyme activitiesof the Daqu samples of five Maotai-flavor liquor distilleries were analyzed, and the relationship between the moldsof Maotai-flavor Daqu and the function of Daqu. The results showed that the saccharification powerof Maotai-flavor Daqu differed significantly 86.91~535.06 mg/(g·h), with insignificant differences in other physical and chemical indices. The activities of various hydrolases were detected in all the five Daqu samples, but the differences of liquefaction enzyme, saccharification enzyme, cellulase, lipase and phytase were relatively large, which might be related to the mold species in Daqu. In addition, the analysis of the enzyme-producing activity of the mold isolated from Daqu revealed that the isolated molds could produce a variety of enzymes, and the activities of saccharification enzyme (0~1724.56 U/g) and neutral protease (0~2671.00 U/g) were different. Aspergillus sp. mainly produced saccharification enzyme, protease and pectinase, Mucor sp. Mainly produced saccharification enzyme and protease, Rhizopus sp. mainly produced saccharification enzyme, Penicillium sp. mainly produced lipase and pectinase, indicating that molds play an important role in the formation of the enzymatic system in Daqu.
    14  Quality Analysis of Semen coicis Yoghurt Fermented by Human Lactobacillus reuteri
    ZHU Xiao-li YANG Zhou-jie WEN An-yan QIN Li-kang
    2021, 37(3):97-105. DOI: 10.13982/j.mfst.1673-9078.2021.3.1052
    [Abstract](226) [HTML](342) [PDF 1.08 M](832)
    Abstract:
    Adlay and milk were used as raw materials and the adlay-yoghourt was synergistically fermented by Lactobacillus reuteri, Streptococcus thermophiles and Lactobacillus bulgaricus. Its physicochemical indexes, texture, taste properties and volatile flavor compounds were detected. Results showed that the content of lactic acid in adlay-yoghourt was the highest and ranged from 11.40~18.38 mg/mL, followed by citric and tartaric acids. However, the chewiness (0.45 mJ), cohesion (0.73 ratio) and gluing properties (0.05 N) of the yogurt (RTB) synergistically fermented by the three strains were significantly higher than that of the yogurt (RT、RB and TB) fermented by the other two strains (p<0.05). The response values of sour, sweet, salty, bitter and umami taste of different products were different (p<0.05). The biggest difference was found in sour. The yogurt synergistically fermented by three strains had the strongest sweet and umami taste, and had the weakest bitter, astringent and sour taste. The maximum 82 kinds of flavor substances were found in the yogurt synergistically fermented by three strains and their contents was 1331.74 ng/mL. The contents of alcohols (167.69 ng/mL), acids (467.33 ng/mL), acids (597.48 ng/mL), and ketones (597.48 ng/mL) of the adlay-yoghourt synergistically fermented by three bacteria were higher than that of the adlay-yoghourt synergistically fermented by two bacteria. In conclusion, the adlay-yoghourt synergistically fermented by tree bacterial showed better texture properties, moderate taste, higher kinds of volatile compounds and content than those of yogurt fermented by 2 strains. As a new type of probiotic milk product, the yogurt synergistically fermented by3 strains might have great market potential.
    15  Screening and Characteristics of Lactic Acid Bacteria for Preparing γ-Aminobutyric Acid-rich Yogurt
    SUN Shi-xin LI Ke LUO Peng-fei YU Lan-xiu MO Xiao-ye SUN Hai-yan ZHANG Li-Jun LIU Dong
    2021, 37(3):106-114. DOI: 10.13982/j.mfst.1673-9078.2021.3.0807
    [Abstract](361) [HTML](289) [PDF 17.90 M](701)
    Abstract:
    In order to obtain lactic acid bacteria within food safety level, which is needed for preparing gamma-aminobutyric acid (GABA)-rich yogurt from dairy-based substrates, 171 lactic acid bacterial strains were isolated from fermented food in domestic market as tested strains. Species identification of these strains was conducted by 16S rDNA sequence analysis, and GABA yield after-fermentation was determined by improved HPLC. After primary screening in broth medium and rescreening in dairy-based medium, one strain of Lactococcus Lactis subsp. lactis (No.4043) was obtained, which had high-yield potential of GABA in dairy-based medium. Inoculated into 10% (W/V) skimmed reconstituted milk with sodium L-glutamate of 2 g/L, the yield of GABA was about 0.39 g/L after fermenting by single strain at 30 ℃ for 48 h, which ranked relatively high level at present. 18 kinds of sugars such as glucose, galactose, etc., couldbe metabolizedby strain No.4043.Inoculated intoM17 medium at 30 ℃, the logarithmic growth period was 2~8 h. The strain No.4043 was weak in continuous acid-producing capacity, showed good acid tolerance in the environment with pH 3~5.5, good in auto-aggregation but poor in bile salt tolerance.Surface hydrophobicity and adhesion of the strain No.4043 were weak, leading to difficulties in adhering and colonizing in human intestinal effectively, which was not suitable to be probiotics in gastrointestinal tract. The strain No.4043 could be used as a starter for producing yoghurt rich in GABA by mixed fermentation of multiple strains.
    16  Glycine Reduces the Histamine Content in the Fast-fermented Shrimp Paste by Compound Bacteria
    YU Jing YANG Xi-hong XIE Jing-wen LU Kuan XIE Wan-cui
    2021, 37(3):115-123. DOI: 10.13982/j.mfst.1673-9078.2021.3.0707
    [Abstract](329) [HTML](234) [PDF 25.01 M](758)
    Abstract:
    Reducing histamine and improving flavor are the bottleneck problems in the industrialization of fast-fermented shrimp paste. In order to explore the inhibitory effect of glycine on the production of histamine in the fast-fermented shrimp paste and the effect on the flavor quality of the shrimp paste, this study used fresh shrimp heads as raw materials, with Pichia gilliermondii, Aspergillus niger and Lactobacillus planticola (1:3:5) as a compound fermentation agent. The changes in histamine content after the addition of glycine were examined, with the effects on the physicochemical properties and safety indicators during fermentation being tracked. The quality and flavor of shrimp paste were analyzed by sensory evaluation and electronic nose. The results showed that the addition of glycine had a significant inhibitory effect on histamine production. The addition of 2%, 4% or 6% of glycine to the shrimp paste reduced the histamine content by 29.53%, 31.16% and 34.98%, respectively. With an increase in the amount of glycine added, the content of AA-N in the shrimp paste increased. As the pH decreased, the total number of colonies was maintained in the range of 3.77~4.15 lg (cfu/g), while the product safety remained good. When the addition amount of glycine was 4%, the sensor response values of sulfur compounds and aromatic compounds increased, which enhanced the characteristic flavor of shrimp paste and improved the nutritional value and sensory flavor. The quantitative addition of glycine not only improves the flavor quality of the product, but also reduces the histamine content to a certain extent to ensure product safety.
    17  Optimization and Kinetics of the Degradation of Flavonoids and Polysaccharides in Seabuckthorn Fruit
    Jialiexi•Manafu WANG Jian-hua Ayizukeri•Esimayi Abulikemu•Wubulida
    2021, 37(3):124-131. DOI: 10.13982/j.mfst.1673-9078.2021.3.0785
    [Abstract](325) [HTML](295) [PDF 20.71 M](802)
    Abstract:
    In this study, we chose seabuckthorn fruit as raw material. Based on the single factor expriment, three factors including storage time, ultrasonic time and extraction temperature were selected as independent variables, and the degradation rate of flavonoids and polysaccharides was selected as response value. Then, the degradation rate of flavonoids and polysaccharides in seabuckthorn fruit was optimized by response surface method. We found that the optimal degradation conditions of flavonoids were: storage time of 10 days, ultrasonic time of 114 min, and extraction temperature of 73.62 ℃; for polysaccharide: storage time of 10 days, ultrasonic time of 115.2 min, and extraction temperature of 71.30 ℃. The degradation rates of flavonoids and polysaccharides were 70.11% and 67.30%, respectively. The variation law and degradation kinetics of flavonoids and polysaccharides in seabuckthorn fruit with various factors were studied, and a storage period prediction model was established. The results showed that in 5~10 days of the storage time, the degradation of flavonoids and polysaccharides fits with the first order kinetics model, and the degradation activation energy Ea is 63.53 and 68.40 kJ/mol. This study could provide a theoretical basis for the selection of storage period of seabuckthorn fruit.
    18  Comparison of the Total Phenolics, Total Flavonoids and Antioxidant Activities in Potato Tips of Different Varieties
    CHEN Peng-feng MEI Xin HUANG Shi-rong CAI Fang WANG Shao-hua HE Jian-jun SHI Jian-bin SUI Yong CAI Sha XIONG Tian CHEN Xue-ling FAN Chuan-hui
    2021, 37(3):132-138. DOI: 10.13982/j.mfst.1673-9078.2021.3.0697
    [Abstract](398) [HTML](261) [PDF 9.06 M](1462)
    Abstract:
    In order to screen out potato tips with a higher antioxidant activity and provide a theoretical basis for the development and utilization of potato tips, the contents of total phenolics and total flavonoids in the leaves, stem tips, stalks and stems of four varieties of sweet potato tips (Fushu7-6, Fushu18, Ningcai and 7001) were compared in this study. Using DPPH· scavenging rate, total reducing power, ·OH scavenging capacity and superoxide anion radical scavenging capacity as indicators of antioxidant activity, the correlation between the antioxidant activity of each part of the four varieties and its phenolic substance content was examined. Experiments showed that the total phenolic contents of the four kinds of potato tips ranged from 4.42 to 98.15 mg GAE/g DW, with the total flavonoid content in the range of 0.97~36.17 mg RE/g DW, DPPH· scavenging rate in the range of 5.13~53.1 mg trolox/g, the total reducing power in the range of 5.65~115.00 mg Vc/g, the ·OH scavenging capacity in the range of 0.04~0.06 (Vc mg/g), and the superoxide anion scavenging capacity in the range of 27.11%~54.50%. Among the four parts, the contents of phenolic substances and associate antioxidant activities in leaves and stem tips were generally higher than those of other parts. Among the 4 kinds of potato tips, only the total flavonoid contents in Fushu 7-6 and Fushu 18 leaf samples were insignificantly different, and the ·OH scavenging capacities of the stem tips and stalks of all varieties differed insignificantly; the contents of total phenolics and total flavonoids were positively correlated with the corresponding antioxidant activity. All in all, there were some differences in the total phenolic content and total flavonoid content as well as their antioxidant activities of different varieties and different parts of potato tips. After screening, it was concluded that the 7001 variety of potato tip had the highest phenolic content and antioxidant capacity, which may be used as a resource of high-quality antioxidants.
    19  Optimization of Ultrasonic Assisted Extraction of Volatile Oil from Aucklandia odorifera and Its Chemical Components Analysis
    YE Yao-hui MA Ya-nan QIAO Xin
    2021, 37(3):139-146. DOI: 10.13982/j.mfst.1673-9078.2021.3.0845
    [Abstract](274) [HTML](201) [PDF 25.84 M](776)
    Abstract:
    In this work, response surface methodology (RSM) was used to optimize the ultrasonic assisted extraction of volatile oil from Aucklandia odorifera. On the basis of single factor experiment, the box Behnken response surface method was used to design the experiment. The yield of Aucklandia volatile oil was used as the response index and the effects of liquid-solid ratio, extraction temperature and ultrasonic time on the yield of Aucklandia volatile oil were investigated. The volatile oil extracted by ultrasonic under the optimal extraction process was analyzed by GC-MS, and the peak area normalization method was used. Their content was also determined. The results showed that the optimum technical parameters were as follows: the ratio of material to liquid of 1:20, the extraction temperature of 50 ℃ and the ultrasonic time of 36 min. The yield of volatile oil under this condition was 5.60%, which was closeto the theoretical value of 5.70%. The results showed that this method was more accurate. GC-MS analysis indicated that a total of 35 compounds were identified, accounting for 92.03% of the total volatile oil components. The components with high contents were caryophyllin, (-)-isoaromatic hydrocarbon-(V), 1-methylcycloheptene, 1,4-cyclobutadiene, 4-(1-methylamyl)-1-cyclohexene-1-carboxylaldehyde, 11,11-dimethyl spirocyclic [2,9] dodecane-3,7-diene. The content of 1,4-cyclobutadiene was highest. Therefore, the technology of ultrasonic assisted extraction of volatile oil from Radix Aucklandiae could beaccurate and reliable, and the ultrasonic assisted method could be applied to the extraction of volatile oil from Radix Aucklandiae. GC-MS method could provide a good basis for the quality identification of Radix Aucklandiae.
    20  Effects of Modified Atmosphere Packaging Combined with Electron Beam Irradiation on Preservation of Mussel Meat
    JIANG Hui-liang WANG Zheng-yun YANG Xu ZHAN Yue-ping XING Chao YANG Qing DUAN Jia-wei
    2021, 37(3):147-153. DOI: 10.13982/j.mfst.1673-9078.2021.3.0780
    [Abstract](243) [HTML](314) [PDF 735.97 K](552)
    Abstract:
    In order to study the effects of modified atmosphere packaging combined with electron beam irradiation on preservation and quality of mussel meat, the air-modified atmosphere ratios were set as 100% CO2 in Group A, 70% CO2 + 30% N2 in group B, 50% CO2 + 50% N2 in group C, 30% CO2 + 70% N2 in group D, and 50% CO2 + 30% N2 + 20% O2 in group E, two control groups were set at the same time. Electron beam irradiation at 5 kGy dose was carried out after modified atmosphere packaging, after the treatment, the mussel meats were ice storage experiment. The results showed that modified atmosphere packaging combined with electron beam irradiation can greatly inhibit the growth of microorganisms of mussel meat during ice storage, reduce the production of total volatile base nitrogen, and at the same time, effectively slow down the fat oxidation caused by electron beam irradiation, reduce the TBA value of mussel meat. Compared with the control group 1, the shelf life of mussel meat during ice storage was prolonged by 17, 17, 15, 13 days and 16 days by each modified atmosphere packaging groups, respectively. When the CO2 content is higher than 50%, the preservation effect of mussel meat has little difference (p>0.05), and 20% O2 can improve the color degree of mussel meat and improve the sensory quality. In general, the preservation effect of 50% CO2 + 30% N2 + 20% O2 modified atmosphere packaging combined with electron beam is better for mussel meat.
    21  Effects of Supplemented with Potato Flake on Properties of Dough
    XU Dan ZHAO Yu-ci ZENG Fan-kui LIU Gang
    2021, 37(3):154-162. DOI: 10.13982/j.mfst.1673-9078.2021.3.0855
    [Abstract](253) [HTML](326) [PDF 18.36 M](640)
    Abstract:
    The effect of potato flake (PF) on the properties of dough and the nutritional quality of cupcakes was investigated. In this work, potato flake (PF) was incorporated in cupcakes at different concentration (10%, 30%, 50%). Compared with the control group, the protein content of the potato flake-added cupcakes increased by 11.04~16.55%, the fat content was reduced by 3.10~13.18%, and the total dietary fiber increased by nearly 1.00~1.50 times. All PF cupcake formulations exhibited higher comprehensive nutritional value in terms of minerals and amino acids. Supplementation of potato flake decreased the score of sensory evaluation, especially at ratio of 30% and 50%. The replacement of wheat flour with the potato flake reduced the height and springiness of cake and enhanced the hardness significantly. The height and springiness were reduced by 2.36~14.91% and 9.55~18.98%, respectively. Their hardness increased by 0.43~42.49%. Thermomechanical properties of the dough demonstrated that the water absorption rate and stability time increased. The protein weakening and the gelatinization properties of starch in mixed dough were significantly affected by potato flake. The microstructure of the dough was characterized by confocal scanning laser microscopy (CSLM) and the results showed that the addition of PF could destroy the continuity of the gluten protein network.
    22  Comparison of the Quality Characteristics of Hempseeds from Different Growing Regions
    WANG Shi-lian RUAN Zheng LI Bian-sheng
    2021, 37(3):163-170. DOI: 10.13982/j.mfst.1673-9078.2021.3.0233
    [Abstract](369) [HTML](484) [PDF 13.96 M](931)
    Abstract:
    In this study, hempseeds from six main growing regions of China were selected: Anhui Lu'an (ALHS), Guangxi Bama (GBHS), Gansu Tianshui (GTHS), Hebei Baoding (HBHS), Heilongjiang Suihua (HSHS), and Yunnan Dayao (YDHS). The appearance, physical indicators, basic nutrients, total phenolic contents and total flavonoid contents, as well as the antioxidant activities (as free radical scavenging capacity and ferric ion reducing power),of these hempseeds were investigated. The correlation between nutritional components and antioxidant activity was also analyzed. The research results showed that YDHS had the highest mass (7.29 g/ 100 grains) protein content (19.18 g/100 g), total sugars content (4.98 g/100 g) and total amino acid content (28.92 g/100 g). GBHS had the highest kernel yield (54.92%) and ALHS had the highest fat content (53.06%) GTHS had the highest ash content (4.86 g/100 g) and the lowest kernel yield (45.57%). The hempseeds from various regions contained large amounts of macro element P (1050.51~1260.09 mg/100 g) and trace element Fe (6.93~9.83 mg/100 g), with their total phenolic contents and total flavonoid contents in the range of 1.71~2.57 mg GAE/g and 0.41~2.92 mg QE/g, respectively (the highest contents of total phenolics and total flavonoids were with YDHS). The results of antioxidant tests showed that in terms of DPPH free radical scavenging ability and FRAP, YDHS had the highest values (1.92 μmol TE/g and 8.89 μmol FE/g, respectively) and GBHS had the lowest values (1.29 μmol TE/g and 4.69 μmol FE/g, respectively).Positive and significant correlations were found between total phenolic contents of hempseeds and their antioxidant activities (p<0.05). Base on the comprehensive analyses of the main components and antioxidant activities of hempseeds, the quality of YDHS was the best.
    23  Process Optimization of Soluble Dietary Fiber from Roxburgh rose Pomace Fermented by Agrocybe aegerita and Its Evaluation on the Moistening and Laxative Function of Mice
    ZHANG Xiang LI Li-lang YANG Juan YANG Xiao-sheng WANG Yu WANG Li LIAO Xiu LI Quan-li LI Qi-ji WANG Dao-ping YANG Yan LI Chun-yan GE Li-juan
    2021, 37(3):171-180. DOI: 10.13982/j.mfst.1673-9078.2021.03.0852
    [Abstract](455) [HTML](230) [PDF 43.06 M](877)
    Abstract:
    Rosa roxburghii pomace was fermented by Agrocybe aegerita to increase the content of soluble dietary fiber (SDF). The function of moistening intestines and defecation of Rosa roxburghii pomace was also investigated, which could provide the basis for the comprehensive development and utilization of dietary fiber of Rosa roxburghii pomace. Methods: on the basis of single factor and Box-Behnken experimental design, the fermentation medium was optimized to improve the yield of SDF. KM mice were randomly divided into blank control group, model control group, positive control group, SDF low (ig. 0.25 g/kg·bw), medium (ig. 0.5 g/kg·bw) and high-dose group (ig. 1 g/kg·bw). The constipation model was established with compound diphenoll. After continuous intragastric administration for 14 days, the effect of the intragastric infusion on the moistening and laxative function of mice was analyzed by measuring the propulsion rate of small intestine ink, the time of the first black stool, and the number and weight of the first black stool within 6 h. Results: the optimum preparation conditions were as follows: calcium chloride of 1%, potassium chloride of 0.6%, sugar of 4% and ratio of liquid to material of 7:1. Under these conditions, the SDF yield of Rosa roxburghii pomace was 5.86%, which was 68.78% higher than that of unfermented pomace. The results of animal experiments showed that on the basis of successful constipation model, all SDF dose groups could improve the constipation of mice, and the effect of high dose group was the most obvious. Conclusion: the preparation technology of SDF from Rosa roxburghii pomace fermented by Agrocybe aegerita was reasonable, economic and convenient, and the product could have a good effect of moistening and laxative.
    24  Preparation Technique of Gypenoside Pellets
    ZHAO Meng-na MIAO Yi-ye YU Fan LIU Qi
    2021, 37(3):181-186. DOI: 10.13982/j.mfst.1673-9078.2021.3.0836
    [Abstract](245) [HTML](236) [PDF 28.91 M](756)
    Abstract:
    The preparation technique of gypenoside pellets was investigated in this work. The optimum technical parameters for producing gypenoside pellet were obtained by single factor test. The extrusion frequency, spheronization frequency, and spheronization time were used as the influencing factors and the roundness of the pellets as the evaluationindex. The blank and gypenoside pellets were prepared by extrusion sphemnization and fluid-bed coating technology, respectively. Micromeritic properties and in vitro release of pellets were determined. The properties of gypenoside pellets were evaluated by infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and scanning electron microscope (SEM). The results showed that the extrusion velocity, spheroization velocity and spheroization time were 30 Hz, 50 Hz, 4 min, respectively. The cumulative amount of gypenoside pellets was 95.98±8.92%, indicating the product complied with the requirements in pharmacopoeia. The friability, the roundness and the angle of repose of gypenoside pellets were 0.90%, 0.80±0.17, 38.70°. The pellets were uniform in diameter, round and smooth, and the fluidity was good. The results of electron microscopy, infrared and X-ray diffraction showed that the pellets were round and the distribution of gypenosides was uniform. It indicated that the process of preparation by extrusion-spheronization and fluid bed coating machines was simple and feasible. It can be applied industrially.
    25  The Effect of Extruded Corn Flour on Wheat Dough Characteristics and Texture Characteristics of Steamed Bread
    SHI Jing-hong ZHU Xiao-bing GUO Shu-wen LI Yun-ling
    2021, 37(3):187-193. DOI: 10.13982/j.mfst.1673-9078.2021.3.0767
    [Abstract](249) [HTML](266) [PDF 812.70 K](937)
    Abstract:
    The effects of different addition amounts of extruded corn meal and corn meal on the farinograph characteristics, gelatinization characteristics of wheat dough and the texture characteristics of steamed bread were compared and studied. The results showed that the addition of extruded corn flour was increased to 25%, the water absorption of the dough was increased by 6.72%, the stabilization time was decreased by 82.64%, the protein weakening degree was increased by 60.78%, the formation time was decreased by 88.37%, the peak viscosity was decreased by 55.60%, and the trough viscosity was decreased by 60.91%, the final viscosity was decreased by 58.41%, the disintegration value was decreased by 34.07%, and the regeneration value was decreased by 50.43%. When the addition of extruded corn meal was 10%, the elasticity, cohesion and recovery of steamed bread were the largest, and the hardness was significantly lower than that of the control (p<0.05), and steamed bread had the best quality with soft taste, smooth texture, refreshing taste and non stick teeth. The added amount of corn meal, the farinograph characteristics and the gelatinization characteristics of the dough were significantly related to the texture characteristics of steamed bread, and the texture characteristics of steamed bread could be predicted by the farinograph characteristics and gelatinization properties of the mixed flour dough. Compared with corn meal, extruded corn meal could increase the water absorption of the dough, enhance the thermal stability of wheat flour, delay the aging of the dough, and improve the quality of steamed bread.
    26  Quality Analysis of Jaboticaba Soaked Wine in Different Soaking Time
    CHEN Yan-xia JIA Xu-chao LI Zhen-wei XIAO Li-mei CHI Jian-wei
    2021, 37(3):194-201. DOI: 10.13982/j.mfst.1673-9078.2021.3.0805
    [Abstract](324) [HTML](258) [PDF 30.66 M](678)
    Abstract:
    Soaked wine was prepared using jaboticaba fruit as raw material, the effect of different soaking time on the active ingredients contents and antioxidant activity of jaboticaba soaked wine were studied. The volatile constituents of jaboticaba soaked wine in different soaked time were also analyzed in order to elucidate its characteristic flavor substance. The results showed that the contents of phenolic compounds in jaboticaba soaked wine were increased with soaking time. When soaked for 3 month, the phenolic contents of jaboticaba soaked wine reached the highest point, the total phenolics, total flavonoid and proanthocyanidins contents of the soaked wine were 1581.11 mg GAE/L, 352.20 mg CE/L and 140.96 mg EPE/L, respectively, which had no significant differences compared with the soaked wine for 6 month. The changes of antioxidant activity of the soaked wine were consistent with the variation trend of phenolic substance content. The ABTS, DPPH and FRAP antioxidant activity of jaboticaba soaked wine were better than other soaked time of wines, the contents are 697.02 mmol TE/L, 428.97 μmol TE/L and 19.97 mmol Fe2+/L, respectively. Alcohols, hydrocarbons, esters and aldoketones were four main identified volatile constituents in jaboticaba soaked wine. Phenethyl alcohol, 1-hexanol, ethyl caprate and ethyl caprylate were important flavor substances of jaboticaba soaked wine. In summary, the best soaking time of jaboticaba soaking wine was 3 months, and the content of active substances, antioxidant activity and flavor substances of soaking wine reached the best level.
    27  Optimization of Ultrasonic Assisted Deep Eutectic Solvents Extraction Process and Antioxidant Activity of Apigenin from Celery Leaves
    LIU Qin ZHOU You CHEN Xuan SHEN Wang-yang ZHUANG Kun LYU Qing-yun
    2021, 37(3):202-211. DOI: 10.13982/j.mfst.1673-9078.2021.3.0821
    [Abstract](341) [HTML](239) [PDF 78.94 M](887)
    Abstract:
    Using celery leaf powder as the raw material and apigenin content as the index, the extraction process of apigenin in celery leaves was optimized. The effects of water content, liquid-to-material ratio, ultrasonic time, and extraction temperature on the apigenin content were investigated in the best deep eutectic solvent. On the basis of single factor, the extraction conditions were optimized by response surface methodology. Using UV-visible spectroscopy and liquid chromatography-mass spectrometry (LC-MS) apigenin was analyzed and verified in the purified extract with macroporous resin. Finally, the antioxidant properties of purified apigenin were studied. The results showed that the efficiency of choline chloride/ethanol was 17.78% higher than that of 80% ethanol in the extraction of apigenin. The optimal conditions were as follow: liquid-to-material ratio of 40:1; water content of 24%; ultrasonic time of 14 min; extraction temperature of 42 ℃. Under these conditions, the apigenin content was 16.87 mg/g. The purified apigenin had a certain scavenging effect on DPPH (IC50=82.44 μg/mL) and ABTS (IC50=148.92μg/mL) free radicals and a strong scavenging ability on hydroxyl (IC50=8.69 μg/mL) free radicals, which were lower than the scavenging ability of ascorbic acid and apigenin standard products. The green and environment-friendly DES can be used to extract apigenin from celery leaves, and the purified apigenin can maintain good antioxidant activity.
    28  Optimization of Pickling Processing of Dried Cowpea
    BU Zhi-bin TANG Dao-bang WEN Jing XU Yu-juan YU Yuan-shan FU Man-qin LI Jun
    2021, 37(3):212-219. DOI: 10.13982/j.mfst.1673-9078.2021.3.0772
    [Abstract](241) [HTML](191) [PDF 2.96 M](728)
    Abstract:
    In order to obtain instant cowpea products, the dried cowpea was used as raw material for soaking and pickling seasoning, the effects of salt, acetic acid, sugar contents and their interactions on total acidity, hardness, L* value and rehydration ratio were explored, and the sensory evaluation of cowpea after soaking and picking was also investigated, using three-factor response surface design. The factors and their interactions between the various factors were analyzed, quadratic regression models were established, multi-objective optimization was performed by entropy weight method, which were verified by applying three optimization methods. The results showed that the established regression model of total acidity, hardness, and sensory evaluation was very significant (p<0.01), the regression model of L* value was significant (p<0.05) and the regression model of rehydration ratio was not significant, suggesting that the model could be used to analyze and predict the pickling processing of dried cowpea parameters. The optimum parameters were 4% of salt content, 1.4% of acetic acid content, and 11.8% of sugar content. With these parameters, the hardness, L* value, rehydration ratio and sensory score were 0.44, 217.03 g, 42.31, 2.94, 85.29, respectively, which were close to the theoretical prediction. The parameters of pickling processing of dried cowpea were optimized by response surface design combined with entropy weight method, which were accurate and reliable, providing a theoretical basis for future study.
    29  Comparison of Ridge Texture Quality in Cattle with Different Cooking Methods
    LIU Fang-yuan ZHOU Wen-da LI Xiao
    2021, 37(3):220-226. DOI: 10.13982/j.mfst.1673-9078.2021.3.0828
    [Abstract](380) [HTML](357) [PDF 7.25 M](795)
    Abstract:
    The effects of different cooking methods on texture parameters and fatty acid content of beef tenderloin were analyzed. Fresh beef sirloin was divided into 5 equal parts to make raw and the cooked beef tenderloin by stewing, roasting, frying and vacuum low-temperature cooking. The effects of cooking methods on water holding capacity, pH, crude protein, crude fat, sensory quality, color, texture parameters and fatty acid content of beef tenderloin were compared. The water holding capacity, pH value, crude protein and crude fat of beef tenderloin were 89.98%, 6.12%, 23.69 mg/g and 23.69 % respectively. The color, tenderness, juiciness and flavor scores of beef tenderness, juiciness and flavor were 8.86, 8.49, 8.84 and 8.62, respectively. The color parameters L* value, a* value, b* value, c* value and H0 value were 45.15, 4.12, 14.82, 15.39 and 15.39, respectively; the texture parameters hardness, viscosity, elasticity, chewiness, cohesion and shear force were 8.36 N, 0.62 N·s, 6.98 mm, 42.68 N·s, 0.42 N/cm2, 25.39 respectively N. The contents of saturated fatty acid and unsaturated fatty acid were higher. There were significant differences in water holding capacity, pH, crude protein, crude fat, sensory quality, color, texture parameters and fatty acid content between vacuum low-temperature beef tenderloin and stewed beef tenderloin, roasted sirloin and fried beef sirloin (p<0.05). According to the above results, there are some changes in microstructure, texture and nutrients between different cooking beef tenderloin and raw beef tenderloin. The microstructure of beef tenderloin under vacuum low-temperature cooking is more complete, which has less effect on texture and better retention of nutrients such as fatty acids, which meets the requirements of beef tenderloin cooking.
    30  Comparison of Drying Characteristics and Quality of Apple Crisps under Three Drying Methods
    HUANG Yan LIN Jun-jin
    2021, 37(3):227-232. DOI: 10.13982/j.mfst.1673-9078.2021.3.0816
    [Abstract](420) [HTML](341) [PDF 2.75 M](831)
    Abstract:
    The effects of different drying techniques on the drying characteristics and quality of apple crisps were investigated. Hot air drying, mid-short wave infrared drying and pulsatile pressure differential flash drying were used to dry apple crisp slices, and the internal temperature, volume and water activity of apple crisp slices were tested by various drying techniques, and the effects of various drying techniques on color parameters and quality of apple crisp slices were tested and compared. The results showed that the L* value of hot air drying technology was 85.69, lower than 93.58 of medium-short wave infrared drying technology, higher than 81.45 of pulsatile differential pressure flash drying (p<0.05); the color difference comprehensive scores of hot air drying, medium-short wave infrared drying and pulsatile differential pressure flash drying were 20.35, 4.52 and 17.53, respectively (p<0.05); the vitamin C mass fraction, rehydration ratio and brittleness values of medium-short wave infrared drying were 2.98, 44.31, 9.05, respectively, higher than 2.71, 4.05 and 8.64 of hot air drying, lower than 3.26, 4.98 and 10.59 of pulsatile differential pressure flash drying (p<0.05). Comparing the three drying methods, it was found that the pulse pressure differential flash drying method had the greatest impact on the quality of apple crisp slices, the medium-short infrared drying method had the greatest impact on the color of apple crisp slices, and the medium-short wave infrared drying method had the greatest impact on the drying characteristics of apple crisp slices
    31  Analysis of Cooking Wine Pickling on Flavor of Fried Tilapia
    ZHAO Xiao-ying QIN Ya-li SHEN Yuan-yuan YIN Hang LIU Xiao-ling
    2021, 37(3):233-240. DOI: 10.13982/j.mfst.1673-9078.2021.3.0733
    [Abstract](339) [HTML](238) [PDF 5.32 M](1005)
    Abstract:
    In this study, sensory evaluation, electronic nose detection and headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) analysis were performed to investigate the deep-fried tilapia pre-marinated with different cooking wines (beer, white liquor and yellow liquor), and compare the flavor differences post deep-frying, and analyze the volatile compounds. The results of sensory evaluation showed that compared with the unmarinated fish sample, the wine aroma and meat flavor of the deep-fried tilapias pre-marinated with a cooking wine were significantly enhanced, while the metallic smell, traditional Chinese medicine smell and earthy odor were significantly weakened. The electronic nose detection revealed that nitrogen oxides, methyls, aldehydes and ketones were important flavor components of deep-fried tilapia, with the volatile components of deep-fried tilapias pre-marinated with different cooking wines being significantly different. A total of 49 substances were detected by GC-IMS, with nonaldehyde, furan, hexanal, benzaldehyde, 2-hexanone, 1-octene-3-ol, ethyl propionate, (E)-2-butenal being the common substances in all analyzed samples (which exhibited oily, fruity, leafy and earthy odors). Nonanal, hexanal, hexanone and pentanol were detected in deep-fried fish pre-marinated with different cooking wines, with their wine aroma and meat flavor being enhanced while the fishy smell being weakened. However, the pungent substances, (E)-2-pentenal and 4,4-dimethylheptane, were detected only in the deep-fried fish pre-marinated with white liquor and yellow liquor, indicating that the marination with white liquor and yellow liquor had negative effects on the flavor of deep-fried fish. The meat flavor and earthy smell were effectively enhanced and reduced, respectively, for the deep-fried fish pre-marinated with beer. Combined with sensory evaluation, the flavor of deep-fried tilapia was enhanced effectively after the marination with beer. The results of this study provide a data basis and theoretical reference for improving the processing of deep-fried tilapia.
    32  Comparative Analysis for the Taste Characteristics of the Umami Dipeptides (Asn-Pro and Ala-His) before and after Structural Modifications and Maillard Reaction
    ZHAO Xuan SU Guo-wan ZHAO Ya-qi HUA Yan-tao LI Bao-yu YIN Kai-dan
    2021, 37(3):241-249. DOI: 10.13982/j.mfst.1673-9078.2021.3.0850
    [Abstract](421) [HTML](371) [PDF 17.44 M](841)
    Abstract:
    The effects of different structural modifications (the change of amino acid sequences, addingumami amino acid residues, cross-connection of the twodipeptides) and Maillard reaction for dipeptides (Asn-Pro and Ala-His) on umami intensities and umami-enhancing effect were investigated. Electronic tongue was used to evaluate the taste characteristics and the umami-enhancing effects on MSG and soy sauce. The results showed that, compared to Ala-His, the umami score of the new dipeptide His-Ala increased from 4.09 to 5.08. His-Ala had a significant umami-enhancing effect on MSG and soy sauce and their umami scores increase by 1.40 and 0.48, respectively. However, compared to Asn-Pro and Ala-His, the umami intensities of the other 9 new peptides (Pro-Asn, Glu-Asn-Pro, Asn-Pro-Glu, Asp-Asn-Pro, Asn-Pro-Asp, Asn-Pro-Asn-Pro, Ala-His-Ala-His, Asn-Pro-Ala-His and Ala-His-Asn-Pro) decreased, and they had significant umami-inhibiting effects on MSG and soy sauce. Maillard reaction could significantly improve the umami intensities of Asn-Pro and Ala-His. The umami scores of Asn-Pro and Ala-His increased from 7.65 and 4.09 to 9.48 and 6.37, respectively. In addition, Maillard reaction products of Asn-Pro and Ala-His had significant umami-enhancing effects on MSG and soy sauce, and their umami scores of MSG increased by 3.96 and 2.29, the umami scores of soy sauce increased by 3.77 and 1.49, respectively. The change of amino acid sequences, addingumami amino acid residues and cross-connection of the twodipeptide could significantly affect the umami taste and umami-enhancing effect of peptides. Maillard reaction could be an effective way to enhance the umami taste and umami-enhancing effect of peptides.
    33  Toxicological Safety Evaluation of Stevioside M
    WU Xin-yue ZHAO Yue SHI Wei-qing LU Luo-ding CHEN Geng WU Jun YU Ping
    2021, 37(3):250-258. DOI: 10.13982/j.mfst.1673-9078.2021.3.0760
    [Abstract](464) [HTML](262) [PDF 1.21 M](911)
    Abstract:
    Stevioside M is a glycoside found in the leaves of Stevia rebaudiana (Bertoni) that has been identified as a potential sweetener. The safety of stevioside M was evaluated according to the national food safety standard, using the acute oral toxicity test, Ames test, mouse bone marrow erythrocyte micronucleus test, mouse spermatocyte chromosome aberration test, and 28 day oral toxicity test. The results showed that the acute oral MTD values of stevioside M in both male and female mice were higher than 10000 mg/kg·bw, indicating that stevioside M was practically non-toxic; the results of Ames test, mouse bone marrow erythrocyte micronucleus test, mouse spermatocyte chromosome aberration test were negative; after the 28 day administration with the basic diet incorporated by stevioside M at designed doses of 2000, 1000 and 500 mg/kg, no significant changes were found in indices including body weight, food intake, food utilization rate, hematology, blood biochemistry and histopathology between each dose group and control group. The No Observed Adverse Effect Level (NOAEL) of female and male mice were 2650 and 2421 mg/ kg·bw. The results indicate that stevioside M has no acute toxicity, genetic toxicity or short-term toxicity, and is therefore of high food safety.
    34  Development and Application of Bovine β-lactoglobulin Gold Immunoassay Strip
    XUE Hai-yan WU Jian ZHANG Ying SONG Hong-xin HE Bao-yuan
    2021, 37(3):259-266. DOI: 10.13982/j.mfst.1673-9078.2021.3.0862
    [Abstract](412) [HTML](232) [PDF 44.36 M](912)
    Abstract:
    A colloidal gold immunochromatography method for the rapid determination of β-lactoglobulin (β-lg) in goat milk and its products were established. Bovine β-lg specific monoclonal antibody (monoclonal antibody, mAb) was prepared by hybridoma technique. Sodium citrate was used to reduce chloroauric acid. The 30 nm colloidal gold particles were formed and used to label bovine β-lgmAb. The competition method was used to develop immunochromatographic strips. A bovine β-lgmAb bag labeled with colloidal gold was placed on the gold label pad. Bovine β-lg and sheep anti-mouse IgG were labeled on nitrocellulose film as detection line (T line) and quality control line (C line), respectively. The optimal encapsulation concentration of bovine β-lg and secondary antibodies were both 1.0 mg/mL. The purity and titer of monoclonal antibody were all above 90% and above 10000 with high specificity. The limit of detection (LOD) of bovine β-lg was 3.13 μg/mL. There was no cross-reaction against other substrate components (bovine α-CN, β-CN, κ-CN, α-LA, BSA). The LOD of whole fat goat milk powder adulterated with skimmed cow milk powder was 0.5 %. The method was used to analyze the commercial goat milk and formula goat milk powder. The results of this method were consistent with that of the commercial ELISA kit. This method could be used to detect bovine β-lg in 5 min, and could be used to on-site rapid detection of sheep milk products.
    35  Determination of Biotin Content in Infant Formulas for Special Medical Purposes
    LU Lan-xiang XUE Xia WEI Li-li ZHAO Hui-nan WU Chuan-xiang DING Yi GONG Pi-xue WANG Jun LIU Yan-ming ZHU Jian-hua
    2021, 37(3):267-274. DOI: 10.13982/j.mfst.1673-9078.2021.3.0570
    [Abstract](192) [HTML](271) [PDF 17.53 M](754)
    Abstract:
    In this study, an ultrahigh liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for the determination of biotin content in infant formulas for special medical purposes. Samples were hydrolyzed with 0.2 mol/L phosphoric acid at 121 ℃ for 30 min before extraction and purification by a biotin immunoaffinity column. The analyte was separated on an acquity UPLC BEH C18 column (100 mm×2.1 mm, 1.7 μm) and eluted with the mobile phase of acetonitrile -0.1% formic acid in a gradient mode. Analyses were performed by the MS/MS system with electrospray ionization (ESI) in a multiple-reaction monitoring (MRM) mode and an internal standard. Under the optimized conditions, a good linear relationship was obtained for biotin in the range of 0.50~50.00 ng/mL (R2=0.9986), with a detection limit of 0.75 μg/100 g. The relative standard deviation of the biotin in the infant formulas for special medical purposes was 0.42%~4.77%, with the recoveries of the spiked samples at three levels were 97.27%~102.06%. The method has several advantages (simple sample processing, high sensitivity and short analysis cycle), therefore, can meet the requirements for determining the biotin content in infant formulas for special medical purposes, providing strong technical support for enterprise quality control and government supervision.
    36  Simultaneous Determination of Organic Acids and Flavonoids in Lonicerae flos by Liquid Chromatography-Tandem Mass Spectrometry
    SHI Ying-zhu HOU Jian-bo XIE Wen QIAN Yan HUANG Chao-qun ZHANG Ya-qin LIU Cui-ping ZHANG Hui
    2021, 37(3):275-285. DOI: 10.13982/j.mfst.1673-9078.2021.3.0814
    [Abstract](227) [HTML](248) [PDF 33.06 M](899)
    Abstract:
    In this paper, an analytical method for the determination of 4 organic acids (chlorogenic acid, caffeic acid, isochlorogenic acid A and isochlorogenic acid C) and 14 flavonoids (quercetin, rutin, luteolin, cynaroside, kaempferol, astragalin, apigenin, rhoifolin, baicalein, baicalin, quercetin-3-methylether, diosmetin, chrysin and kaempferide) in Lonicerae flos were developed and validated by solid phase extraction and liquid chromatography with tandem mass spectrometry (LC-MS/MS). In Lonicerae flos samples, the organic acids and flavonoids were extracted by methanol solution (concentration: 50%), ultrasonication for 40 min, and cleaned up with HLB column by solid phase extraction. The methanol eluent was ?ltered through nylon membrane and analysis by LC-MS/MS. The solution was separated by Mightysil RP-18 column (150 mm × 4.6 mm (i.d), 3 μm) and the quantitative detection was performed on LC-MS/MS by multiple reaction monitoring (MRM) mode under negative electrospray ionization (ESI-). External standard method was used for quantitation analysis. The limits of quantification (LOQs, S/N>10) were 0.005 g/kg~0.05 g/kg. Single-laboratory method validation data were determined, the calibration standard curves concentrations were 0.05~1.00 g/kg and the correlation coefficient of calibration standard curves was more than 0.993. The recoveries were found in the range of 69.2%~116% and the relative standard deviations were 3.3%~12.0% at the respiked levels (10.0, 20.0 and 40.0 g/kg for chlorogenic acid; 1.0, 2.0 and 4.0 g/kg for caffeic acid; 5.00, 10.0 and 20.0 g/kg for isochlorogenic acid A and isochlorogenic acid C; 0.05, 0.10 and 0.20 g/kg for other compounds). This method was suitable for simultaneous determination and confirmation of 4 organic acid and 14 flavonoids in Lonicerae flos. Key words: Lonicerae flos; organic acid; flavonoids; high performance liquid chromatography with tandem mass spectrometry of quantification (LOQs, S/N>10) were 0.005 g/kg~0.05 g/kg. Single-laboratory method validation data were determined, the calibration standard curves concentrations were 0.05~1.00 g/kg and the correlation coefficient of calibration standard curves was more than 0.993. The recoveries were found in the range of 69.2%~116% and the relative standard deviations were 3.3%~12.0% at the respiked levels (10.0, 20.0 and 40.0 g/kg for chlorogenic acid; 1.0, 2.0 and 4.0 g/kg for caffeic acid; 5.00, 10.0 and 20.0 g/kg for isochlorogenic acid A and isochlorogenic acid C; 0.05, 0.10 and 0.20 g/kg for other compounds). This method was suitable for simultaneous determination and confirmation of 4 organic acid and 14 flavonoids in Lonicerae flos.
    37  Preparation of Coupled Armored RNA Reference Material for Norovirus GI/GII
    WANG Ming-qiu YANG Jun CHANG Yu-tong ZHANG Tao LIU Li-juan
    2021, 37(3):286-293. DOI: 10.13982/j.mfst.1673-9078.2021.3.0835
    [Abstract](353) [HTML](246) [PDF 23.56 M](916)
    Abstract:
    To provide a safe and stable reference material for Norovirus nucleic detection, the armored RNA containing target RNA of Norovirus GI and GII based on MS2 bacteriophage was developed in this work. DNA fragments including maturase coding gene, capsid protein coding gene and packing site of MS2 bacteriophage, target cDNA sequence of Norovirus GI and GII were synthesized artificially and then cloned into expression vector pET-28a(+) to construct recombinant plasmid pET-MS2-NoV. After expressed in E. coli BL21 cells by IPTG induction, the expression product was purified by PEG6000, enzyme digestion and molecular sieve chromatography. The purified product, also named AR-NoV, was identified by SDS-PAGE, transmission electron microscopy (TEM) and RT-PCR. The value, homogeneity and stability of the AR-NoV were evaluated. SDS-PAGE analysis showed that with the molecular weight of tar-get protein expressed in BL21 was 10~15 ku, which was consistent with the predicted value. There were no impure proteins and residual nucleic acids in AR-NoV after purification. The AR-NoV presented as spherical VLPs with uniform particle size (about 25 nm) and integrated structure under TEM. The values of GI and GII targets in AR-NoV were (4.04±0.62)×107 copies/μL and (6.16±0.30)×107 copies/μL, respectively. The good homogeneity of AR-NoV was confirmed by single-factor ANOVA test (F
    38  Identification of Honeysuckle Leaves from Four Different Genetic Varieties Based on HPLC Fingerprints
    TONG Kai SHEN Guo-qiang HUANG Yue LI Jie-yuan ZENG Fan-fu YAN Meng GAN Wan-ying LI Dong
    2021, 37(3):294-300. DOI: 10.13982/j.mfst.1673-9078.2021.3.0763
    [Abstract](261) [HTML](272) [PDF 34.00 M](928)
    Abstract:
    The discriminant analysis techniques to distinguish different varieties of honeysuckle leaves were developed based on their differences in chemical composition. Using the leaves of Lonicera macranthoides Hand.-Mazz., L. hypoglauca Miq., Bei Hua 1 L. japonica Thunb. and Si Ji L. japonica Thunb. as experimental materials, high performance liquid chromatography was used to construct chemical fingerprints of honeysuckle leaves. After 34 characteristic chromatographic peaks were extracted, the differences in chemical compositions of the four different varieties of honeysuckle leaves were analyzed and compared in combination with multivariate statistical analyses such as principal component analysis and system cluster analysis. The results revealed that the first two principal components accumulatively represented 51.3% of the original variables, and the principal component score maps of the four different varieties of honeysuckle samples showed relatively independent spatial distribution characteristics. Cluster analysis revealed that the 32 samples were grouped into 4 clusters according to the variety, source and similarity of fingerprints. Compared with Si Ji, Bei Hua 1 resembled the most in chemical characteristics, follow by L. hypoglauca Miq. and L. macranthoides Hand.-Mazz. The ten chromatographic peaks, No. 5, 6, 7 (chlorogenic acid), 8, 11, 14, 16, 18, 24 and 29, in the fingerprint spectra were proved to be the main contributors to the chemical differences among the different varieties of honeysuckle leaves, which can be further developed into stable chemical markers. The HPLC fingerprint and analysis method established in this research can be used for the identification and analysis of different varieties of honeysuckle leaves including Lonicera macranthoides Hand.-Mazz., L. hypoglauca Miq., Bei Hua 1 L. japonica Thunb. and Si Ji L. japonica Thunb.
    39  Nondestructive Identification of Longjing Tea Grade by Fusing Spectral and Textural Feature
    LU Jiang-ming FAN Ting-ting MU Qing-shuang KANG Zhi-long
    2021, 37(3):301-307. DOI: 10.13982/j.mfst.1673-9078.2021.3.0826
    [Abstract](298) [HTML](168) [PDF 6.22 M](964)
    Abstract:
    The rapid and nondestructive identification of Longjing tea grade was of great significance. In this study, support vector machine (SVM) model was respectively established by using hyperspectral imaging technology with six levels of Longjing tea, based on spectral features, texture features and fusion features. First, standard normal variable (SNV) was used to normalize the spectra, extract the spectral features, and establish the SVM spectral model. Then, the high-dimensional hyperspectral data was mapped to the low-dimensional space through the T-distributed and stochastic neighbor embedding (T-SNE) algorithm, and feature images were selected. Gray-level Co-occurrence matrix (GLCM) was applied to extract texture features and establish a SVM image model. Finally, spectral features and texture features were fused at the data level to establish a SVM mixture model. The results showed that the recognition rate of predictive sets based on spectral model was 91.11%, the recognition rate of predictive sets based on image model was 75.42% and the recognition rate of predictive sets based on mixed model was 95.14%. It illustrated that compared with modeling using only spectral or texture information, combining spectral and texture features can improve the accuracy of identification. In order to further improve the performance of the mixed model, artificial bee colony (ABC) algorithm was introduced to iteratively optimize the penalty factor C and kernel function width g of the SVM model, construct the optimal model, and the accuracy of the prediction sets can be reached 98.61%. The study provides a reliable method to improve the rapid nondestructive assessment technology of Longjing tea.

    Editor in chief:李琳

    Inauguration:现代食品科技

    International standard number:ISSN 1673-9078

    Unified domestic issue:CN 44-1620/TS

    Domestic postal code:46-349

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