Volume 36,Issue 11,2020 Table of Contents

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  • 1  Effect of Sarsasapogenin on Lipopolysaccharide-induced BV2 Microglial Subtype M1/M2 Polarization and Sarsasapogenin’s Anti-inflammatory Activity
    YANG Zhi-you HU Xue-qiong
    2020, 36(11):1-8. DOI: 10.13982/j.mfst.1673-9078.2020.11.0406
    [Abstract](920) [HTML](281) [PDF 117.53 M](1199)
    Abstract:
    In this study, the effect of sarsasapogenin (SAR) on the polarization of BV2 microglia from M1 to M2 subtypes induced by lipopolysaccharide as well as the mechanism underlying SAR’s anti-inflammatory activity were investigated. The MTT method was used to evaluate the effect of SAR on the cell viability of microglia. The release of NO was measured by the Griess method. The expressions of TNFα, IL1β, IL6, and IL10 in the cell culture supernatants were measured by the ELISA. The immunocytochemistry method was used to detect the primary microglia M1 and M2 phenotypes, and the expression levels of p65, p38 and PPARγ in microglia. The results showed that SAR (0.01~1 μmol/L) had no effect on the cell viability of BV2 microglia under normal conditions and in LPS (100 ng/mL)-induced inflammatory state, however, high doses of SAR (10 and 25 μmol/L) showed certain cytotoxic effects. Compared with the normal group, LPS-treated model group exhibited an increased level of released NO (by 22.64%), upregulated expressions of TNFα, IL1β and IL6 (by 25.5%, 13.28%, and 48.21%, respectively), downregulated expression of IL10 (by 14.97%), and significantly increased levels of Cox2 positive and Ym1/2 negative expressed M1 microglia. Furthermore, the phosphorylation levels of p38 and p65 were 2.80 times and 1.86 times, respectively, that of the normal group, while the expression of PPARγ decreased by 73.90% in the LPS-treated group. Compared with the LPS model group, the treatment with SAR (0.1 and 1 μmol/L) inhibited the release of NO and secretion of TNFα, IL1β and IL6, and promoted LPS-induced polarization of microglia to M2 phenotype, decreased significantly the phosphorylation of p38 and p65, and up-regulated the PPARγ expression. The mechanisms underlying SAR’s anti-inflammatory function were attributed to the inhibition of the phosphorylation of p65 and p38 via the activation of PPARγ, promotion of the polarization of microglia from M1 to M2 subtype, and reduction of the expression of inflammatory cytokines and release of NO.
    2  Simulated Gastrointestinal Absorption and Metabolite Analysis of Policosanol
    ZHU Yan-jiao LI Peng MENG Fei HUANG Dong-ting HUANG Jun-sheng XIANG Hui
    2020, 36(11):9-16. DOI: 10.13982/j.mfst.1673-9078.2020.11.0465
    [Abstract](201) [HTML](455) [PDF 23.67 M](815)
    Abstract:
    To investigate the gastrointestinal absorption and metabolite analysis of policosanol in vitro and in vivo, single-phase static gastrointestinal simulation system, in situ circulating intestinal perfusion in rats and integral animal experiments were conducted. The results showed that policosanol could be metabolized into three saturated fatty acids including C12:0, C16:0 and C18:0 in the single-phase static gastrointestinal simulation system. Compared with artificial gastric juice, artificial intestinal juice degraded 42% more policosanol, which was converted to the saturated fatty acid. In intestinal system of rat, policosanol could be absorbed easily by the intestinal tract of rats, and the Papp value of policosanol was 7.02×10-2 cm/min. Moreover, policosanol were able to be brokn down into fatty acids and unsaturated fatty acids in the intestinal tract. In the integral animal experiments, at 1, 4, 12 hours after oral gavage of 100 mg/kg policosanol, the alkanol and its metabolites fatty acid could be identified in the serum of mice, which may contribute to regulate serum fatty acid levels and increase the serum level of some unsaturated fatty acids. In summary, our results revealed that policosanol can be metabolized and absorbed by the gastrointestinal tract and can also be detected in the animal body in the form of prototypes or metabolites, affecting the level of fatty acids in the body, which may be the basis for its lipid regulation function.
    3  Improvement Effects of Moringa oleracea Seed Oil on Lipopolysaccharide-induced Intestinal Inflammation in Mice
    ZENG Zhen MO Ming-ming HUANG Yan CHEN Cheng-yu GUI Zhong-yu HE Wen LEI Shao-ling SONG Jia-le
    2020, 36(11):17-23. DOI: 10.13982/j.mfst.1673-9078.2020.11.0543
    [Abstract](423) [HTML](229) [PDF 42.31 M](742)
    Abstract:
    To investigate the effect and mechanism of Moringa oleracea seed oil on LPS-induced intestinal inflammation in male ICR mice, lipopolysaccharide (LPS, 3.5 mg/kg) was injected intraperitoneally to establish the intestinal inflammation model of mice. After 7 days, Moringa oleracea seed oil was administrated by gavage, and corn oil was used as the control. Changes in the body weight and colon tissue of mice were recorded. The levels of cytokines, includingIL-1β, IL-6, IL-18 and TNF-αin serum and colon were measured by ELISA assay, respectively. The mRNA levels of Nlrp3, Asc, Caspase-1 and IL-1β in colon were detected by qRT-PCR assay. Moringa oleracea seed oil significantly inhibited the weight loss and reduced the ratio of colon weight to length in LPS-treated mice (p<0.05). At the same time, the levels of IL-1β (175.44 ng/L and 184.12 ng/L), IL-6(82.12 ng/L and 58.19 ng/L), IL-18(82.91 ng/L and 52.32 ng/L) and TNF-α (51.75 ng/L and 81.31 ng/L) in serum and colon of the model mice were significantly reduced by the intervention of Moringa oleracea seed oil. The level of MPO in colon of model mice was significantly reduced to 86.32 ng/L (vs injury group: 113.59 ng/L, p<0.05). Compared with the injured group, Moringa oleracea seed oil inhibited the mRNA expression of Nlrp3 (39.31%), Asc (42.81%), Caspase-1 (35.59%) and IL-1β (28.62%). These results suggestted that Moringa oleracea seed oil could improve the intestinal inflammation in LPS-treated mice. The mechanism might be due to the reducing the activation of NLRP3 by Moringa oleracea seed oil in LPS-treated mice.
    4  Protective Effect of Ginseng Polysaccharide on Mitochondria in Rats with Coronary Heart Disease
    WAN Hao-fang YING Jia-ni GUAN Yang WANG Lin-yan
    2020, 36(11):24-28. DOI: 10.13982/j.mfst.1673-9078.2020.11.0510
    [Abstract](273) [HTML](277) [PDF 5.27 M](645)
    Abstract:
    The protective effect of ginseng polysaccharide on mitochondria in rats with coronary heart disease was studied. The levels of mitochondrial calcium (Ca2+), succinate dehydrogenase (SDH), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), soluble Fas (FAS), Fas ligand (FASL), b-lymphoma-2 (Bcl-2) and b-lymphoma-2 genes related X protein (Bax) were detected by the intervention of ginseng polysaccharide. The results showed that the concentration of Ca2+ and SDH activity were significantly changed with the increase of the action concentration of ginseng polysaccharide (p<0.05), among which, the Ca2+ concentration and SDH activity in the high dose group were 43.41 μmol/g and 7.21 U/mg respectively. After the action of ginseng polysaccharide, MDA content, GSH PX enzyme activity and SOD activity in high dose group were 4.19 nmol/mL, 132.42 U/mg and 90.18 U/mL respectively, which were significantly different from those in the model group (p<0.05). At the same time, compared with the model group, ginseng polysaccharide significantly reduced the expression levels of FASL, Fas, Bcl-2 and Bax in rats (p<0.05). The expression of FASL, Fas, Bcl-2 and Bax in high dose group was 1.21, 1.09, 1.08 and 1.02, respectively. The results showed that ginseng polysaccharide could improve the blood lipid abnormality in rats with coronary heart disease, and had the function of anti-oxidation and anti-tumor, and had strong protective effect on mitochondria.
    5  Study on the Effect of High γ-aminobutyric Acid-enriched Yoghurt on Sleep and Its Mechanism on Insomnia in Mice
    MO Xiao-ye LUO Peng-fei YU Lan-xiu LYU Le LI Ke LIU Dong SUN Hai-yan
    2020, 36(11):29-35. DOI: 10.13982/j.mfst.1673-9078.2020.11.0484
    [Abstract](597) [HTML](371) [PDF 12.78 M](1076)
    Abstract:
    The effect of high γ-aminobutyric acid-enriched yoghurt on sleep and its mechanism on insomnia was accessed by male ICR mice. Hypnosis test of pentobarbital sodium subthreshold, test of extended pentobarbital sodium sleep time and barbital sodium sleep latency test were conducted to determine the effect of high γ-aminobutyric acid-enriched yoghurt on sleep. PCPA insomnia model was used to explore the influence of high γ-aminobutyric acid-enriched yoghurt on insomnia. The changes of Gly, GABA, 5-HT and Glu in mice brain tissue and serum were measured to determine the mechanism. Compared with control group, 80 and 160 mg/kg high γ-aminobutyric acid-enriched yoghurt could improve 60% sleep rate of mice (p<0.05). 80 mg/kg high γ-aminobutyric acid-enriched yoghurt could extent mice sleep time to 156.20 min (p<0.05). 40, 80 and 160 mg/kg high γ-aminobutyric acid-enriched yoghurt did not affect mice sleep latency. In insomnia experiment, high γ-aminobutyric acid-enriched yoghurt increased the content of GABA and Gly in the brain tissue to 1.10 ng/mL and 28.78 ng/mL, respectively. In conclusion, high γ-aminobutyric acid-enriched yoghurt could improve sleep mainly by affecting the content of GABA and Gly in mice brain.
    6  Inhibitory Effect of Dihydromyricetin on the Proliferation of Osteosarcoma Cells
    CHEN Qing JIA Qiang WANG Huan LI Jia-han YAN Jing WU Lan-jie LI Yi-fei
    2020, 36(11):36-41. DOI: 10.13982/j.mfst.1673-9078.2020.11.0413
    [Abstract](340) [HTML](176) [PDF 47.14 M](810)
    Abstract:
    In the present study, the inhibitory effect and mechanism of action of dihydromyricetin (DHM) on the proliferation of osteosarcoma cells were investigated. The inhibitory effects of DHM solutions at different concentrations on the proliferation of osteosarcoma cells were examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. The influences of DHM solutions at different concentrations on the cell morphology, apoptosis and cell cycle of osteosarcoma cells were examined by an inverted microscope, Hoechst 33258 fluorescent staining and flow cytometry. The expression levels of cell cycle- and apoptosis-related proteins were analyzed by Western Blot method. The results showed that DHM exhibited a significant inhibitory effect on the proliferation of osteosarcoma cells and caused changes in the cell morphology of osteosarcoma cells in a dose-dependent manner, with the half inhibitory concentration (IC50) value as 24.41±1.25 μM. The results of cell cycle experiments showed that after the DHM treatment, the percentage of the cells at G0 phase to the cells at G1 phase (G0/G1) decreased significantly from 60.20% to 21.50%, whereas, the percentage of the cells at G2 phase to the cells at M phase increased from 11.60% to 45.30%, and the expression of cell cycle-related protein, Cyclin B1, decreased, causing the cell cycle arrest. In addition, the ratio of the expression of anti-apoptotic protein, Bcl-2, to the expression of pro-apoptotic protein, Bax, decreased, inducing apoptosis. These findings indicate that DHM has significant anti-proliferation activity against osteosarcoma cells, and may be a potential functional food or drug for the treatment of osteosarcoma.
    7  Polysaccharides Extract from Codonopisis pilosula Enhances the Vitality and Antioxidant Activity of Drosophila melanogaster
    YE Wen-bin HE Yu-peng CHEN Yao-nian YANG Wen ZHAO Qing-fang ZHOU Zi-juan
    2020, 36(11):42-48. DOI: 10.13982/j.mfst.1673-9078.2020.11.0500
    [Abstract](269) [HTML](189) [PDF 1.13 M](753)
    Abstract:
    In order to evaluate the effect of Codonopisis pilosula polysaccharides on the reproduction capability and life span and antioxidant capacity of Drosophila melanogaster, survival experiments were conducted to examine the influences of different concentrations (50, 100, 200, 400 mg/L) of Codonopisis pilosula polysaccharides on the reproduction capability and life span of four generations (F1 to F4), the number of female and male, the survival time in paraquat treatment, and the activity of antioxidant enzyme superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) contents. The results showed that the 400 mg/L dose of CPP could significantly increase the fertility of F1~F4 generations female and male Drosophila generations. Compared with the control, the number of offspring Drosophila generations increased by 150% (p<0.01), and extended the life span of F1 to F4 female and male Drosophila generations. The lifespan of offspring Drosophila generations increased by 34.07% compared with the control (p<0.01). Compared with the control, the survival time of F1~F4 generations female and male fruit flies under the acute oxidative damage of paraquat were also prolonged (7.06 and 6.28 h (p<0.01), respectively). At the same time, the activities of SOD and CAT in Drosophila generations increased (p<0.01), and the content of MDA was reduced (p<0.01). It indicated that high dose of Codonopisis pilosula polysaccharides could increase the reproduction capability and extend the life span of F1 to F4 female and male Drosophila generations. After treated with the high dose of Codonopisis pilosula polysaccharides, the survival time in paraquat treatment was improved, the contents of MDA in the Drosophila generations was reduced and the activity of SOD and CAT or the antioxidant capacity increased rapidly, exhibiting a dosage effect., and the sex ratio of female and male Drosophila generations maintain 1:1.
    8  Resveratrol Improved the Immune Function of Rats Exposed to T-lymphocyte Injury Induced by Samray
    ZHUO Wei-wei
    2020, 36(11):49-53. DOI: 10.13982/j.mfst.1673-9078.2020.11.0492
    [Abstract](223) [HTML](221) [PDF 700.21 K](541)
    Abstract:
    To study the effect of resveratrol on immune function of rats exposed to t-lymphocyte injury induced by tpa, 50 SPF-level SD healthy rats were randomly divided into blank group, model group, low-dose group (20 μmol/L), medium-dose group (40 μmol/L) and high-dose group (80 μmol/L), to establish the tlymphocyte injury model rats induced by γ-ray. After successful modeling, rats were given resveratrol by gavage. According to the experimental design, the proliferation rate of T cells, nuclear factor kB of T lymphocytes (NF-kB) expression, level of T lymphocytes (CD3, CD4, CD8), interferon-γ (IF-γ), interleukin-12 (IL-12), interleukin-4 (IL-4), and interleukin-3 (IL-3) were detected. The results showed that the proliferation rate, CD3, CD4, CD8 and CD4/CD8 of resveratrol group (40 μmol/L) were 2.15%, 54.15%, 35.11%, 21.45% and 2.11, respectively. The levels of IF-γ, IL-12, IL-4 and IL-3 secreted by T cells were 21546.15 pg/mL, 212.15 pg/mL, 645.56 pg/mL and 221.15 pg/mL, respectively, which were significantly different from those of other experimental groups (p<0.05). It is understood that resveratrol can significantly inhibit and regulate T cells and improve the immune function of rats.
    9  Screening and Evaluation of Lactobacillus Strains for Improving Metabolic Syndrome
    YANG Ge ZHANG Lin WANG Chao ZHAO Yu-juan ZHAO Zi-jian GAO Lei CUI Wei-dong LI Sheng-yu
    2020, 36(11):54-60. DOI: 10.13982/j.mfst.1673-9078.2020.11.0440
    [Abstract](208) [HTML](308) [PDF 6.77 M](850)
    Abstract:
    In order to screen the lactic acid bacteria that can relieve the metabolic syndrome, a mouse model with metabolic syndrome was established using high-fat and high-fructose diet. The body weight, serum lipid levels, inflammatory factors, and insulin resistance index were detected to evaluate the effects of six strains of Lactobacillus on improving metabolic syndrome. The results of APICHL50 sugar fermentation and 16S rDNA partial sequence alignment results showed that six strains of Lactobacillus were identified as Lactobacillus plantarum. Furthermore, compared with the model group, all the strains could reduce body weight, regulate lipid disorders, down-regulate inflammation levels, improve insulin resistance. However, there were obvious differences in the functions among six strains. Our results indicated that L. plantarum S9 could obviously improve the insulin resistance index (29.26%), markedly decrease TC (13.22%), TG (9.97%), LDL-C (21.31%) and FFA (32.33%) levels and increase HDL-C (15.91%) in serum. Moreover, L. plantarum S9 could significantly decline the inflammatory factor levels in serum (IL-1β 12.03%, TNF-α 20.46%, IL-6 15.90%, CRP 37.28%, LPS 10.01%). Therefore, all above results imply that L. plantarum S9 can be used as a potential functional strain for the prevention and treatment of metabolic syndrome.
    10  UV-C-irradiated Gallic Acid Solution (UVC-GA) Inhibited the Growth of E. coli O157:H7 on the Surface of Oyster Flesh
    GAN Hui GUAN Yi-yin WANG Yuan-yuan RUAN Zhi-de YANG Qiong CHENG Xiu-li LU Xiao-hua MA Hua-wei LYU Min
    2020, 36(11):61-69. DOI: 10.13982/j.mfst.1673-9078.2020.11.0421
    [Abstract](177) [HTML](180) [PDF 39.29 M](737)
    Abstract:
    A novel ultraviolet-irradiated gallic acid antibacterial solution (UVC-GA) was developed using a new method of light-induced enhancement of GA antibacterial activity through exposing gallic acid (GA) to UV-C for 12 h. The GA solution obtained after UVC-GA for 12 h could reduce E. coli O157: H7 by 3.57 log cfu/mL (which was 22.33 times higher than that of GA. The antibacterial activity of UVC-GA solution was affected by the irradiation duration, wavelength and pH. After 60 min of irradiation, UVC-GA reduced E. coli O157: H7 by 4.42 log cfu/mL, which was significantly higher than UVB-GA and UVA-GA by 64.25% and 223.47%; The reduction of E. coli O157: H7 by UVC-GA at pH 3 was 94.23 and 87.62 times, respectively, higher than those at pH 7 and pH 11. The addition of benzenesulfinic acid (BSA) to UVC-GA lowered E. coli O157: H7 to 0.63 log cfu/mL (p<0.05), indicating that quinone compounds in UVC-GA enhanced its antibacterial activity. The addition of reactive oxygen species (ROS) quencher to UVC-GA significantly (p<0.05) reduced the antibacterial effect by 4.56% and 8.15%, respectively, compared with those of the DMSO-treated group and the mannitol-treated group (p<0.05). The generation of hydrogen peroxide and the increase of E. coli O157: H7-induced oxidative stress indicated the contribution of ROS to the antibacterial effect of UVC-GA. The results showed that UVC-GA could effectively inhibit E. coli O157:H7 on the surface of oyster flesh, and correctly treated UV-C irradiated GA can be used as a novel antimicrobial solution for sterilization of oyster products and other foods.
    11  Release and Changes in Activity of Essential Oils from Guava Leaves by Solid-state Fermentation
    WEI Wei-qian LUO You HU Xi FANG Zheng WU Zhen-qiang
    2020, 36(11):70-79. DOI: 10.13982/j.mfst.1673-9078.2020.11.0126
    [Abstract](295) [HTML](264) [PDF 15.29 M](885)
    Abstract:
    In this work, the release of essential oil from guava leaves during the solid-state fermentation of guava leaf substrate with mixed strains, Monascus anka and Bacillus megaterium, were investigated. The results showed that the 20 main components in the guava leaf essential oil remained unchanged after fermentation, but the rate of essential oil extraction increased significantly and reached 6.53 times that of the unfermented group after 10 days of fermentation. Tissue structure analysis combined with enzymatic analysis inferred that glycosidases destroyed the cell walls, glandular trichomes, and vacuoles of guava leaves, and cleaved the intersugar linkages and glycosidic bonds, to promote the release of aromatic compounds from guava leaves. After fermentation, the DPPH radical scavenging ability of 2,2-diphenyl-1-picrylhydrazyl in guava leaf essential oil decreased by 26.15%, but the ABTS+ radical scavenging ability and 5-lipoxygenase inhibitory capacity and tyrosinase inhibitory capacity of 2,2'-azino-bis(3-ethylbenzothiazoline-6- sulfonic acid) diammonium salt increased by 32.66%, 18.73% and 7.87%, respectively (with the corresponding IC50 values reaching 20.47±2.49 mg/mL, 23.21±1.92 μg/mL and 107.41±3.48 μg/mL, respectively). Overall, fermentation can improve the antioxidant and biological activities of the guava leaf essential oil. The results may provide a new insight into the efficient extraction of fruit leaf essential oils and a pathway for the development of novel natural health products.
    12  Isolation, Identification and Carbon Source Metabolism Analysis of Three Strains of the Hami Melon (Cucumis melo var. saccharinus L.) Rot Pathogens
    WANG Yu-bin FENG Yu-qing LU Rong-rong HE Chao-jun ZHAO Xiao-yan WANG Dan MA Yue ZHANG Chao
    2020, 36(11):80-86. DOI: 10.13982/j.mfst.1673-9078.2020.11.0403
    [Abstract](220) [HTML](179) [PDF 26.50 M](824)
    Abstract:
    Hami melon is extremely prone to spoilage during storage and transportation. In this study, 3 strains of pathogens were isolated and purified using the tissue separation method from the Hami melon that decayed naturally during storage. Based on the identification by morphological observation, ITS sequencing analysis and phenotypic analysis, the CYS strain was Aspergillus ochraceus (JN942915.1), CJS was Trichothecium roseum (KM877467.1), and CWS was Diaporthe sp. (LT965977.1). The analysis of growth and metabolism of 3 strains of rot-causing pathogens with the Biolog-FF technique revealed that there were 44 main carbon sources of CYS metabolism, with the strongest ability to metabolize carboxylic acid followed by amino acids and sugars. There were 22 main carbon sources of CJS metabolism, with the strongest ability to metabolize amino acids followed by carboxylic acid and sugars. There were 41 main carbon sources of CWS metabolism, with the strongest ability to metabolize sugars followed by carboxylic acid and amino acids. Therefore, the metabolism of the pathogens existing on the surface and in the environment of fruits and vegetables could be accelerated due to the presence of substances conducive to the growth of these pathogens (such as sugars, amino acids and carboxylic acids), causing acceleration of the deterioration of fruits and vegetables. The conclusion of this study can further reveal the infection mechanism of pathogenic bacteria and provide a theoretical basis for the study of storage technology.
    13  Evaluation of Antioxidant and Anti-inflammatory Activities and Analysis of Chemical Components in Different Extraction Parts of Fresh Longya Lilium
    ZHAO Kang-hong ZHOU Feng YAN Si-en LIU Dong-bo XIE Hong-qi
    2020, 36(11):87-97. DOI: 10.13982/j.mfst.1673-9078.2020.11.0469
    [Abstract](269) [HTML](212) [PDF 15.63 M](1040)
    Abstract:
    In this paper, the oxidative stress model induced by H2O2 and the inflammatory model induced by lipopolysaccharide (LPS) were used to evaluate the antioxidant and anti-inflammatory activities of the extracts from 80% ethanol solution of fresh Longya Lilium, and the chemical components of the extraction parts with significant activities were analyzed by ultra-high pressure liquid chromatography tandem quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS).The results showed that the water saturated n-butanol extract part(50 μg/mL) had the strongest antioxidant capacity, which increased the activity of antioxidant enzymes in RIN cells by 2.78 times (SOD), 3.79 times (GSH), 6.27 times (CAT); the chloroform extract part (50 μg/mL) had the strongest anti-inflammatory activity, and the inhibition rates of NO, TNF-α, IL-6 in RAW 264.7 cells were 55.21%, 20.86% and 84.95% respectively. In addition, the antioxidant activity of water saturated n-butanol extraction part was stronger than that of chloroform, and the anti-inflammatory activity of chloroform extraction part was stronger than that of water saturated n-butanol. The water saturated n-butanol part is mainly composed of 12 compounds (including 1 lignan, 5 phenylpropanoic acids and 6 steroidal saponins), the chloroform part is mainly composed of 14 compounds (including 7 phenylpropanoic acids, 1 diosgenin and 6 steroidal saponins), and 4 compounds are common components. Therefore, phenylpropanoic acid and steroidal saponins may be the material basis of fresh Longya Lilium's antioxidant and anti-inflammatory activities, and most of them are concentrated in the extraction parts of water saturated n-butanol and chloroform. These results provide theoretical reference for further elucidating the material basis of Lilium's biological activities and its development and utilization.
    14  Analysis of Nutrition Composition of Hemibarbus Hybrid
    KE Huan YANG Zhuang-zhi CHEN Jian CHEN Xia GUO Tian-rong WU Wen-lin ZHOU Yi ZHANG Yin
    2020, 36(11):98-103. DOI: 10.13982/j.mfst.1673-9078.2020.11.0499
    [Abstract](251) [HTML](237) [PDF 771.64 K](650)
    Abstract:
    To evaluate the difference of nutritional value between the new breed hybrid fish and its parents, the basic nutrients, amino acids and fatty acids of the three Hemibarbus and tilapia were compared. Results showed that the three Hemibarbus fish had the characteristics of high ash content and low fat, the basic nutrition composition of the three Hemibarbus fish was similar; the content of flavor amino acids and essential amino acid of the three Hemibarbus fish were higher than those of tilapia. There was no significant difference (p>0.05) in content of glycine and essential amino acids among the three Hemibarbus fish. The fatty acid type of the three Hemibarbus fish was similar to that of tilapia, but the content of polyunsaturated fatty acids (PUFA) were higher than that of tilapia. PUFAHemibarbus labeo (47.24%) was significantly higher (p<0.05) than that of PUFAHemibarbus hybrid (42.24%) and PUFAHemibarbus maculates (35.02%), PUFAHemibarbus hybrid (42.24%) was significantly higher (p<0.05) than that of PUFAHemibarbus maculates (35.02%). The order of the content of total eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were as follows: Hemibarbus labeo (18.39%)> Hemibarbus hybrid (13.71%)> tilapia (11.90%) > Hemibarbus maculates (8.90%). In conclusion, comparing to the other two Hemibarbus fish, the protein nutritional value of the Hemibarbus maculatus was higher, the fatty acid nutritional value of the Hemibarbus labeo was higher, the nutritional value of the Hemibarbus hybrid was more comprehensive.
    15  Composition Analysis of Three Plant Essential Oils and Comparison of Their Antibacterial Activities
    SUN Da XIA Bing
    2020, 36(11):104-113. DOI: 10.13982/j.mfst.1673-9078.2020.11.0436
    [Abstract](441) [HTML](225) [PDF 7.09 M](1266)
    Abstract:
    In order to compare the constituents and antibacterial activities of three essential oils from lemongrass, jasmine and turmeric, gas chromatography-mass spectrometry (GC-MS) was used in this experiment to analyze the composition of the samples, and then filtration with filter paper and serial dilution method were applied to examine the antibacterial ability of these samples. The experimental results indicated that the main characteristic components of turmeric essential oil were curcumin (12.87%), aromatic curcumin (38.53%) and gingerone (20.36%), with the antibacterial components including camphene (2.38%); The main components of lemongrass essential oil were limonene (12.72%), citral (8.52%) and terpinolene (17.53%), with citral (8.52%) being the antibacterial component; The main components of jasmine essential oil were linalool (11.88%), benzyl acetate (9.31%) and cinnamaldehyde (31.87%), with cinnamaldehyde (31.87%) as the component with antibacterial activity. The inhibition zone diameters of lemongrass essential oil, jasmine essential oil and turmeric essential oil against Staphylococcus aureus were 20.10 mm, 10.22 mm and 7.84 mm, respectively, with that of the positive control Nisin being 13.40 mm. The above three essential oils all contained substances with certain antibacterial activities, and exhibited antibacterial properties in the inhibition zone test. Compared with Nisin, the inhibition effect decreased in order of lemongrass essential oil > jasmine essential oil > turmeric essential oil.
    16  Effects of Low-oxygen Storage on the Quality and Physiological Disorder of ‘Yali’ Pear
    FENG Yun-xiao HE Jin-gang CHENG Yu-dou LI Li-mei GUAN Jun-feng
    2020, 36(11):114-120. DOI: 10.13982/j.mfst.1673-9078.2020.11.0344
    [Abstract](184) [HTML](333) [PDF 21.64 M](965)
    Abstract:
    To explore the effects of low-oxygen storage on the fruit quality and physiological disorder of ‘Yali’ pear, fruit quality, skin darkening, core browning and chilling injury of ‘Yali’ pear during cold storage (0 ℃) and shelf life (20 ℃), in 1%, 3%, 5% and 10% of O2 as well as in air (as the control), were examined. The results showed that the low-oxygen treatments (1%, 3%, 5% and 10% of O2) delayed the decrease of fruit hardness, inhibited the increase of soluble solid contents, and delayed the decreases of Vc content and titrable acid content. Compared with the control, the skin darkening index decreased by 38.81% (in 3% O2), 48.11% (in 5% O2) and 33.51% (in 10% O2), but increased by 7.74% in 1% O2. The core browning index was reduced by 51.64% and 39.31% in 5% and 10% O2, respectively, but increased by 44.43% in 1% O2. The treatments with 1% and 3% of O2 increased the chilling injury of the fruits, with the chilling injury index being 0.65 and 0.09, respectively, at Day 180. Further investigations revealed that the treatment with 3%, 5% and 10% of O2 delayed the decrease of the total phenolics content in the fruit core, and significantly inhibited the increases in the relative conductivity of the peel and the PPO activities of the fruit core. Comprehensive analysis showed that the treatment with 5% of O2 offered the greatest effects on keeping the storage quality and reducing physiological diseases of Yali pear.
    17  Comparison of Preservation Effects of Different Preservatives on Blueberry
    ZHANG Li-hui LI Zhi-hang LIAO Li QIAO Yu ZHANG Jin-mu CHEN Xue-ling
    2020, 36(11):121-129. DOI: 10.13982/j.mfst.1673-9078.2020.11.0457
    [Abstract](542) [HTML](313) [PDF 54.32 M](680)
    Abstract:
    In order to extend the storage time of blueberry, five different preservative methods, including eugenol slow-release preservation, clove extract, ethanol fumigation, controlled atmosphere preservation and eugenol slow-release combined with controlled atmosphere preservation, were used to study the effects on the quality of blueberry during the storage. The results showed that the five preservative methods significantly inhibited the rot and quality loss rate of blueberry fruit, and effectively alleviated the decrease of soluble solids, titratable acid, reducing sugar, Vc content, inhibited the increase of MDA content. At the same time, SOD vitality of the blueberry fruit was maintained at high lever. When blueberries were stored for 49 days, the rot rate and weight loss rate of blueberries treated with eugenol slow-release and controlled atmosphere were 50.13% and 2.71%, respectively; the soluble solids, titratable acid, reducing sugar, Vc and anthocyanin contents were 7.06%, 7.26 g/kg, 4.14 g/100 g, 0.93 g/100 g and 117.48 mg/100 g, respectively; MDA content was 26.84 nmol/g and SOD activity was 176.34 U/mL. Compared with the control sample, eugenol slow-release combined with controlled atmosphere can extend the storage of blueberries for 14 days. The results indicated that the five kinds of preservation treatments have a certain preservation effect on blueberries, and the eugenol slow-release combined with controlled atmosphere was the best preserved method.
    18  Improvement of the Storage Quality of Mulberry Leaf Vegetable by Vacuum Precooling Combined with Modified Atmosphere Packaging Under Simulated E-commerce Logistics Conditions
    YANG Teng-da CHEN Yu-long ZENG Fan-kun YE Ming-qiang WANG Ling LUO Zheng CHEN Fei-ping
    2020, 36(11):130-136. DOI: 10.13982/j.mfst.1673-9078.2020.11.0371
    [Abstract](200) [HTML](220) [PDF 4.71 M](930)
    Abstract:
    To explore a more effective preservation technology to prolong the shelf-life of mulberry leaf vegetables, the effects of vacuum precooling combined with modified atmosphere packaging (MAP) under different conditions on the appearance quality, weight loss rate,headspace gas partial pressure, respiration intensity and some nutrients of mulberry leaf vegetables were studied under simulated e-commerce logistics conditions, by comparing modified atmosphere packaging only (as the control). The results showed the two-sided effects of vacuum precooling on the quality of mulberry leaf vegetable. A lower precooling treatment (5 ℃) accelerated the deterioration of the quality of mulberry leaf vegetables thereby was not conducive to vegetable storage and preservation. Appropriate precooling treatment (15 ℃) could delay significantly the deterioration of appearance quality, inhibit respiration and reduce the loss of nutritional value. Vacuum precooling (15 ℃) combined with MAP could prolong the shelf life of mulberry leaf vegetables not less than 96 h under simulated e-commerce logistics conditions (non-cold chain). The respiration intensity of the mulberry leaf vegetables treated by vacuum precooling combined with MAP was remarkably lower than that of control (597.20~308 mg CO2/(kg h) vs 464.66~298 mg CO2/(kg h)), with the gas atmosphere in the package being maintained better (O2: 1.44~3.13 kPa, CO2: 7.30~7.56 kPa). After the 96-h storage, the mulberry leaf vegetables treated by vacuum precooling combined with MAP had a L* value higher than 0.52° and a* value lower than 0.41°, with the retention rates of polyphenol and vitamin C being increased by 7.89% and 7.00%, respectively. Accordingly, an appropriate vacuum precooling-MAP treatment could delay the decline of the postharvest quality of mulberry leaf vegetables. The research results not only provide an effective solution strategy for preserving mulberry leaf vegetables during logistics transportation at room temperature, but also are of great significance to enrich the existing technical theories for postharvest preserving mulberry leaf vegetables.
    19  The Preservation Effects of Microporous Spontaneous Controlled Atmosphere Packaging on Guanyu Loquat
    MA Jia-jia SUI Si-yao SUN Ling-xiang HUANG Gui-li WANG Yu-ning
    2020, 36(11):137-146. DOI: 10.13982/j.mfst.1673-9078.2020.11.0485
    [Abstract](291) [HTML](443) [PDF 19.76 M](729)
    Abstract:
    In order to study the effect of packaging methods on the qualities and flavor characteristics of loquat, the effects of biaxially stretched polypropylene (BOPP) microporous packaging and polyethylene (PE) self mucous ordinary packaging on the sensory, physical and chemical qualities and volatile substances of guanyu loquat were investigated under the cold storage environment of temperature (6±0.5) ℃, relative humidity 90%~95%. The results showed that compared with PE self-mucosal ordinary packaging, the microporous packaging had better spontaneous air conditioning effect (O2: 12.40%, CO2: 7.80%), the weight loss rate, decay rate ,commodity rate of loquat fruit were 1.24%, 10.00%, 90.00% respectively. The soluble solids, glucose, fructose, sorbitol and sour taste were 5.67%, 4.13%, 4.51%, 51.34% and 25.04% higher than those of ordinary packaging respectively; the sucrose content, sweet and fresh taste were 30.99%, 20.32% and 27.17% lower than those of ordinary packaging respectively. The volatile substances produced by microporous packaging were less than those in ordinary packaging, and the contents of hexanal and e-2-hexanal which contributed the most to the aroma value were 1.10 times and 39.59% higher those in ordinary packaging respectively. Conclusion: microporous packaging can delay the decline of physical and chemical quality of loquat fruit, reduce the loss of soluble monosaccharide and sorbitol, delay the change of taste index, inhibit the increase of volatile substances in the late storage period, retain the content of main aroma components, improve the marketability and delay the decline of loquat flavor. It has potential market prospect as a postharvest fresh-keeping retail packaging of loquat fruit.
    20  Alleviating Action of Sodium Selenite on Immunological Stress in Ictalurus punctatus during Temporary Cultivation
    ZU Xiao-yan LI Hai-lan ZHAO Ya-jing LIU Shu-yan LI Pai LIAO Tao XIONG Guang-quan
    2020, 36(11):147-153. DOI: 10.13982/j.mfst.1673-9078.2020.11.0439
    [Abstract](196) [HTML](180) [PDF 903.90 K](580)
    Abstract:
    To determine the effect of sodium selenite (Na2SeO3) on blood indexes of Ictalurus punctatus during the process of temporary cultivation, in this study, the Ictalurus punctatus were randomly divided into four groups (n=20 with fish water ratio of 130 g: 1 L in each group), the Na2SeO3 was added to the water, and the initial concentrations were 0 (control group), 0.1, 0.2, 0.5 mg/L, respectively. Temporary cultivation for 4 days with fasting, the related serum biochemical indexes such as liver metabolism, energy metabolism, immune protein and oxidative stress were detected every day. The results showed that, at the initial stage of cultivation, the alanine aminotransferase (ALT) and glutamic oxaloacetic transaminase (AST) in serum of 0.1 mg/L Na2SeO3 group were significantly lower than that of 0.2 mg/L group and 0.5 mg/L group (p<0.05). With the increase of cultivation days, ALT and AST activities in serum in the Na2SeO3 groups were decreased. And the activities of ALT and AST in serum of 0.1 mg/L Na2SeO3 group were significantly reduced in the 2 days of temporary cultivation (p<0.05) compared with the control group, amounted to 75.39% and 170.13% respectively. With the prolongation of cultivation time, the concentration of MDA in the 0.1 mg/L Na2SeO3 group was significantly lower than that in the 0.2 mg/L and 0.5m/L groups. Moreover, the blood glucose in the 0.1 mg/L Na2SeO3 group increased significantly (p<0.05) at first and then decreased significantly (p<0.05) with the increase of cultivation time, which was contrary to the trend of the control group, delaying the rate of blood glucose decline. The above results showed that when the fish water ratio was 130 g:1 L, the initial concentration of Na2SeO3 in water was 0.1 mg / L for about 2 days, which could alleviate the stress response, delay the process of blood glucose reduction caused by fasting, and improve the antioxidant capacity of Ictalurus punctatus.
    21  In Vitro Gastrointestinal Digestibility and Antioxidant Activity of Fermented Millet
    WU Hui-lin LI Miao-yun ZHU Yao-di ZHAO Gai-ming GAO Hao-yuan XIAO Kang REN Hong-rong ZHAO Li-jun
    2020, 36(11):154-162. DOI: 10.13982/j.mfst.1673-9078.2020.11.0419
    [Abstract](204) [HTML](321) [PDF 3.00 M](921)
    Abstract:
    Taking millet (sour meat’s excipient) as the research object, the changes of postprandial glucose as well as the digestibility, particle size and antioxidant activity of millet during the simulated gastrointestinal digestion were analyzed. The results showed that with the prolongation of postprandial time, the blood glucose level first increased within 0~30 min, then decreased rapidly after 30 min. The postprandial blood glucose level after 240 min was basically stable (22.79 dl/mg, which was insignificantly (p>0.05) different from the blood glucose level at 23.42 dl/mg after 300 min. With the progress of digestion of the sample through the oral cavity, stomach and small intestine, the particle size of the product decreased gradually, whilst the digestibility of protein and fat increased gradually (the digestion rate of protein was 69.45% and digestion rate of fat was 29.88% when the digestion reached the gastrointestinal stage). In the antioxidant tests, the contents of polyphenols and polypeptides increased significantly with the progress of digestion (p<0.05), with the highest content of polyphenols as 6.06 mg/100 g, and the highest content of polypeptides as 5.21 mg/mL. The antioxidant capacity increased gradually with the progress of digestion. The maximum scavenging rates of OH radical, DPPH radical and ABTS radical in the digestive juice were 81.63%, 78.12% and 7.62%, respectively.protein and fat digestion rates, while having higher polyphenol and polypeptide contents and antioxidant activities (OH, DPPH and ABTS radical scavenging rates). These results provide a basis for the development of millet fermented products, and further studies are needed on the nutrition and health of millet fermented products.
    22  Preparation of Vitamin C/β-Carotene Composite Liposomes with Their Surfaces Modified by Sodium Alginate-Chitosan
    LIU Xin LUO Zhi-gang LI Xiao-lin
    2020, 36(11):163-169. DOI: 10.13982/j.mfst.1673-9078.2020.11.0060
    [Abstract](326) [HTML](377) [PDF 34.97 M](1013)
    Abstract:
    In order to enhance the stability of liposomes, the surface of Vitamin C (Vc)/β-carotene (βC) composite liposomes (L) were modified by chitosan and sodium alginate with layer-by-layer technique, to prepare monolayered chitosan liposomes (C-L) and bilayered sodium alginate-chitosan liposomes (S-C-L). The encapsulation efficiency, particle size distribution, microscopic morphology, formation mechanism and storage stability of the liposomes were studied. The results showed that there was no significant change in the encapsulation efficiency of the active components in the liposomes before and after a liposomal surface modification, with the encapsulation efficiency of Vc and βC higher than 75% and 95%, respectively. The results obtained dynamic light scattering (DLS) and transmission electron microscopy (TEM) showed that after surface modification, the z-average particle size of liposomes increased from 250.1 nm to 541.9 nm. The Zeta-potential measurements and FT-IR analysis showed that chitosan and sodium alginate could successfully modify the surface of liposomes via electrostatic interactions, without changing the internal structure of the liposomes. The results of storage stability tests showed that the retention rates of Vc and βC in C-L and S-C-L were about 2%~10% higher than that of L under the same conditions, indicating that the storage stability of liposomes was enhanced after surface modification.
    23  Improvement of Different Post-mortem Conditioning Span Treatments on Eating Quality of Soft-boiled Chicken
    HU Yang-jian DENG Shao-lin ZHANG Xin-yue ZHAO Jun-feng YIN Dong-shan HAN Min-yi XU Xin-lian
    2020, 36(11):170-179. DOI: 10.13982/j.mfst.1673-9078.2020.11.0505
    [Abstract](264) [HTML](303) [PDF 35.65 M](801)
    Abstract:
    Selecting an appropriate post-mortem span to let broiler carcass experience conditioning is of great importance to the quality of broiler processed product. Instrumental analysis methods and sensory evaluation were applied to investigate the effects of different post-mortem conditioning span on eating quality of soft-boiled chicken, so as to provide credible data and theory for future industrial production of soft-boiled chicken. In this experiment, spotted-brown broilers from same specification were slaughtered and divided into three groups depend on their different post-mortem span (1 h, 2 h and 3 h), then boiled to produce soft-boiled chicken under factory conditions. The skin color, pH, texture, volatile flavor compound and sensory quality of these chicken were evaluated, then principal component analysis (PCA) was carried out to explain the interrelationships between instrumentanalyzed indexes and sensory evaluation scores, also interrelationships in the instrument analyzed indexes themselves. The results of volatile flavor compound from GC-IMS analysis were subject to PCAto illuminate the variation of volatile flavor compound among soft-boiled chicken from different groups. Results show significant differences (p<0.05) in skincolor (L*: 73.24~76.47, b*: 29.73~37.05), texture and relative content of volatile flavor compound of thesesoft-boiled chicken. Moreover, compared with the two groups with short post-mortem span, the longer post-mortem span group shows better results in sensory evaluation (71.00~85.50) and in this experiment can provide theoretical basis for industrial practice and quality improvement of soft-boiled chicken. relative content of volatile flavor compound. The variation of each product quality index among different post-mortem span that are expounded
    24  Improving the Stability of Protein-based High Internal Phase Emulsions by Two-step Emulsifying Method
    ZHANG Li-li TANG Chuan-he
    2020, 36(11):180-187. DOI: 10.13982/j.mfst.1673-9078.2020.11.0007
    [Abstract](394) [HTML](373) [PDF 65.63 M](1188)
    Abstract:
    In this study, taking native bovine serum albumin (BSA) as the protein model, two-step emulsification was used to improve the stability of protein-based high internal phase emulsions (HIPEs) without causing protein denaturation. Firstly, an initial emulsion (micro-droplets) with φ as 0.2 and d4,3 (volume-averaged diameter) in the range of 17.60~0.42 μm, was prepared by high-energy homogenization (high speed homogenization 30000 r/min, ultrasonication and microfluidization). Then, HIPEs (φ=0.8) were fabricated using micro-droplets as the Pickering stabilizer through low-energy homogenization (high speed homogenization at 13500 r/min). HIPEs with different properties were prepared by changing the protein concentrations and the homogenization energy for the preparation of initial emulsions. The interfacial protein adsorption rate (AP%), particle size of the initial emulsions were characterized, while their appearance, microstructure and rheological characteristics were examined before and after a 20 day storage at room temperature. Finally, a comparative experiment to remove free proteins and a thermal stability test of HIPEs were conducted. It was found that the most suitable protein concentration was 1 wt%, even at a concentration as low as 0.1 wt%, HIPEs remained stable and still after being turned upside down. The stability of HIPEs was effectively improved when the d4,3 of micro-droplets was not higher than 2.16 μm. The results showed that the presence of micro-droplets significantly improved the storage stability and thermal stability of HIPEs.
    25  Optimization of Processing Technology of Noodle with High Content Coarse Grains
    YAN Mei-jiao LI Yun-long LI Hong-mei YI Xin SUN Yuan-lin ZHAO Lin
    2020, 36(11):188-195. DOI: 10.13982/j.mfst.1673-9078.2020.11.0550
    [Abstract](346) [HTML](345) [PDF 48.84 M](848)
    Abstract:
    In order to improve the content of coarse grains and nutritional value of noodles, the technology of vacuum cold extrusion was used to mix oat, barley and tartary buckwheat flour with 20% wheat flour as base material. The cooking loss rate and sensory score were evaluated. Though controlling water addition, water temperature, wake-up time, coking loss rate was set as response value, and the technology of high content coarse grains noodles was optimized using response surface method. The result show that the flour ratio of wheat, oat, barley and tartary buckwheat was 2:3.75:1.25:3. Noodles made with this ratio had better sensory quality (sensory score was 87) and lower cooking loss rate (10.04%). Under this ratio, processing technology of noodle with high content coarse grain was optimized. The best processing conditions of noodle with high content coarse grain were as follows: Water addition of 45%, water temperature of 58 ℃ , wake-up time of 10.5 min. Under these conditions, the coking loss rate of noodle was 8.01%. Compared with that of mixed noodles, the cooking loss rate was reduced by 2.03%, and the quality was improved. In conclusion, the vacuum cold extrusion technology could be used to produce noodles with high content coarse grains and good taste.
    26  Changes of in Vitro Simulated Gastrointestinal Digestion on the Antioxidant Activity of Walnut Flower
    LI Ru-rui CHEN Xin RU Yue-rong MU Hong-lei GAO Hai-yang WANG Zhen-xing KAN Huan
    2020, 36(11):196-201. DOI: 10.13982/j.mfst.1673-9078.2020.11.0443
    [Abstract](406) [HTML](251) [PDF 614.94 K](586)
    Abstract:
    To investigate the influence of in vitro digestion on the antioxidant component and activity of walnut flower, the walnut flower extract (WFE) was subjected to mimic mouth, gastric and gastrointestinal digestion. The total phenolic content (TPC) and total flavonoid content (TFC) of WFE were determined by folin-ciocalteu method and sodium nitrite- aluminum nitrate colorimetric method. Antioxidant activities of WFE were evaluated using DPPH radical scavenging capacity, ABTS radical scavenging capacity, and ferric reducing antioxidant power (FRAP) assays. Results showed that TPC, TFC and antioxidant activity of WFE decreased during oral, gastric and intestinal digestion.After digestion, TPC decreased by 81.21% (from 3.30 to 0.62 mg/mL), while TFC decreased by 99.31% (from 2.88 to 0.22 mg/mL). The simulated digestion significantly decreased the antioxidant activity of WFE, the IC50 value increased from 211.02 to 424.45 μg/mL for DPPH radical scavenging activity, from 220.63 to 421.82 μg/mL for ABTS radical scavenging activity, and from 106.13 to 40.47 μg/mL for FRAP value. In the stage of gastric and intestinal digestion, the acid and alkali environment and digestive enzymes destroyed the antioxidant components of WFE, which led to a significant reduction in the antioxidant activity.
    27  Process Optimization of Rosa roxburghii Tratt - Hericium erinaceus Beverage Fermented by Lactic Acid Bacteria
    ZHANG Jun YU Ting XU Hao-xiang HUANG Hai-lun HU Ping TAN Shu-ming WANG Xiao-yu FENG Dan-dan
    2020, 36(11):202-211. DOI: 10.13982/j.mfst.1673-9078.2020.11.0451
    [Abstract](313) [HTML](236) [PDF 63.75 M](836)
    Abstract:
    In order to find the optimal process parameters of Lactobacillus paracasei SR10-1 fermented Rosa roxburghii Tratt-Hericium erinaceus compound beverage, in this study, the Box-Behnken center combination design was used, compound beverage was produced by the raw materials of Rosa roxburghii Tratt, Hericium erinaceus and granulated sugar, which were inoculated with Lactobacillus paracasei SR10-1 for compound fermentation. On the basis of single factor experiment, the effect of mass ratio of Rosa roxburghii Tratt and Hericium erinaceus, the inoculation amount of Lactobacillus paracasei SR10-1, fermentation time and impact of sugar addition were selected as variables. The response surface analysis was performed with the polysaccharide content and sensory score as the response values. The results showed that the optimal fermentation conditions were determined as follows: the combined mass ratio of Rosa roxburghii Tratt-Hericium erinaceus was 2.6:1, the inoculation amount of Lactobacillus paracasei SR10-1 was 2%, the fermentation time was 4.6 days, and white granulated sugar addition amount was 18.5%. Under the optimized conditions, the polysaccharide content of fermented beverage was 9.10 mg/mL and the sensory score was 89.34 points, Vc content was 243.57 mg/100 mL, SOD content was 5377.64 U/g tissue wet weight.The color of the fermentation beverage was bright and shiny, the aroma was rich and coordinated, the flavor was unique, and the nutrition was enriched. It has good value of industrialization development.
    28  Changes in the Water-holding Capacity of Pork during Post-mortem Aging
    XUE Pan-pan ZHOU Xiao-yan WANG Qiu-yu ZHANG Hai-feng
    2020, 36(11):212-216. DOI: 10.13982/j.mfst.1673-9078.2020.11.0438
    [Abstract](352) [HTML](271) [PDF 4.82 M](1223)
    Abstract:
    The longissimus dorsi muscle of Sanyuan pig was used as the research object, the changes in the cooking loss rate, drip loss rate, storage loss rate, shear force and pH value were examined at the 4 th, 8 th, 12 th, 16 th, 20 th, 24 th, 28 th, 32 th and 36 th h, and the changing trend of water-holding capacity of pork during post-mortem aging was studied. The results showed that the cooking loss rate increased first and then decreased, with the maximum value of 32.80% at the 20 th h; the storage loss rate and drip loss rate decreased first, then increased and finally decreased; the shear force increased significantly within the range of the 4 th~12 th h, with the maximum of 62.29 N at the 12 th h. The overall pH trend showed a decrease, an increase, then a decrease, and finally level-off. Among which, there were a significant negative correlation between the aging time and shear force (p<0.01), a very significant negative correlation between the pH value and cooking loss rate (p<0.01), and a strong correlation between the drip loss rate and storage loss rate (p<0.01). In summary, the water-holding capacity of pork showed a changing trend of a decrease followed by an increase within 36 hours of post-mortem aging, with the peak point of rigor mortis (postmortem rigidity) within the 12 th~16 th h before the post-rigor phase and maturation stage. The results of this study can provide basic indices and reference basis for further in-depth studies on the changing trend and mechanism of pork tenderness during postmortem aging.
    29  Comparative Analysis of Different Oven Humidity on Nutritional Quality and Edible Quality of Steam-assisted Roasting Broccoli
    LIANG Yong-xue LIU Dong-mei WANG Yong CHEN Dong-po ZHOU Peng
    2020, 36(11):217-225. DOI: 10.13982/j.mfst.1673-9078.2020.11.0445
    [Abstract](247) [HTML](202) [PDF 589.55 K](1364)
    Abstract:
    This study selected broccoli to explore the effects of different humidity (0%, 25%, 50%, 75%, 100%) on the nutritional quality and sensory properties of broccoli. The results showed that Vc content in fresh broccoli was significantly higher than that of steam-assisted roasting broccoli at 0%~25% cavity humidity, but not significantly different from that of steam-assisted roasting broccoli at 50%~100% cavity humidity. After steam-assisted roasting, the polyphenol and chlorophyll decreased significantly; but as the cavity humidity increased, the retention rate increased accordingly. With the increase of the cavity humidity, a* of broccoli decreased significantly and b* changed not significantly. Compared to 25%~100%, the L* of steam-assisted roasting broccoli at 0% cavity humidity was significantly high. Hardness and firmness of steam-assisted roasting broccoli at 0% cavity humidity were largest, however, there were no significant differences among other cavity humidity samples. Finally, the comprehensive results of sensory quality, nutritional index and sensory evaluation showed that the optimum cavity humidity of steam-assisted roasting broccoli is 75% or 100%.
    30  TG Enzyme-sodium Caseinate-sodium Alginate to Improve the Quality of Conditioned Steak
    YANG Hong-ji QIAN Zhi-long LI Hao MA Chuan-song JIN Jie WANG Ling-bo
    2020, 36(11):225-235. DOI: 10.13982/j.mfst.1673-9078.2020.11.0476
    [Abstract](293) [HTML](276) [PDF 6.66 M](852)
    Abstract:
    To improve the quality of regulated steaks, promote its integrity and solve the problem of its uneven quality, in this study, glutamine transaminase (TG) concentration (0.10%, 0.20%, 0.30%, 0.40%, 0.50%), sodium caseinate concentration (0.20%, 0.40%, 0.60%, 0.80%, 1%), sodium alginate concentration (0.10%, 0.20%, 0.30%, 0.40%, 0.50%), and the action time (2 h, 4 h, 6 h, 8 h and 10 h) were selected for single factor experiment. The test conditions were determined based on the steak shear force, shape retention, slicing, cooking adhesion and comprehensive effect. The optimal processing parameters to improve the quality of the steak were determined by response surface method as follows: the mass fraction of TG enzyme was 0.32%, the mass fraction of sodium tyrosinate was 0.40%, the mass fraction of sodium alginate was 0.31%, the acting time was 6 h. Under these conditions, the sensory score of the steak was 4.85, the predictive value of sensory score was 4.89,with a relative deviation of 0.82%; and the tensile strength was 818.85 g·s, the predicted tensile strength was 829.43 g·s, with a relative deviation of 1.27%. The results can provide a basis for quality control and standardized production of regulated steak production enterprises.
    31  Volatile Components and Storage Stability of Fish Oil Microcapsules Prepared by Different Methods
    CUI Ting-ting JIA Ai-rong BAI Xin-feng SHI Ya-ping LIU Xue LIU Chang-heng
    2020, 36(11):236-243. DOI: 10.13982/j.mfst.1673-9078.2020.11.0471
    [Abstract](300) [HTML](289) [PDF 19.65 M](768)
    Abstract:
    The effects of different drying processes on volatile components and storage stability of fish oil and microcapsules were investigated. Fish oil was used as the core material, konjac glucomannan and soybean protein isolate were used as the wall material. The fish oil microcapsules were prepared by spray drying and freeze drying. The results showed that a total of 80 volatile components were identified, 42, 41 and 20 of which were detected in fish oil, spray drying microcapsule and freezedrying microcapsules respectively. But only 4 common compounds were identified in them, including Ethyl tetradecanoate, Ethyl hexadec-9-enoate, 1-methyl-4-propan-2-ylbenzene and 2-p-Tolyl-1-propene. In accelerated storage experiments, peroxide value (POV) growth rate of two kinds of fish oil microcapsules was lower than that of fish oil. With the storage time extended to 30 d, the POV values of fish oil, spray drying microcapsule and freeze drying microcapsule were 15.65 mmol/kg, 8.89 mmol/kg and 8.14 mmol/kg, respectively. In addition, the embedding rate of spray drying fish oil microcapsule declined faster than freeze drying fish oil microcapsules, the embedding rate was 34.29% and 40.90%, respectively. Comprehensive analysis showed that the two methods could delay the oxidation of fish oil and mask the bad flavor, but freeze-drying was better, which had a certain reference significance for the preparation of fish oil microcapsules.
    32  Changes in Aroma Components of Jiuzhi Tangerine Peel Pericarpium Citri Reticulatae during Pickling and Storage Determined by Gas Chromatography-mass Spectrometry/Olfactory
    MA Rui-ting WU You CAO Yong HU Hai-e LI Xue-li HE Li-ping ZHANG Jin-tao CHEN Zhe-ling LIAO Zi-yuan
    2020, 36(11):244-254. DOI: 10.13982/j.mfst.1673-9078.2020.11.0430
    [Abstract](208) [HTML](261) [PDF 26.15 M](773)
    Abstract:
    The changes in aroma components of Jiuzhi tangerine peel Pericarpium citri reticulatae during pickling and storage were examined to provide a basis for the preservation, storage and quality control of the tangerine peel. In this research, the untreated dry peel (raw material) and the pickled peel stored for different time periods after pickling were used as test samples, and gas chromatography-mass spectrometry/olfactometry (GC-MS/O) was used to analyze and identify the volatile components of untreated and treated peels during storage. The obtained relative odor activity values were subjected to statistical analysis. A total of 58 volatile components were detected, with the relative contents of 6 compounds exceeding 1%. The main components were 38 olefin compounds, with D-limonene having the highest relative content (81.84%). After 8 months of storage, the types of volatile compounds were the least. With the formation of the characteristic flavor of Jiuzhi tangerine peel Pericarpium citri reticulatae, the contribution of different compounds to the flavor changed greatly. There were 25 types of compounds with aroma characteristics with 12 compounds being the key flavor compounds. Aldehydes, phenols and olefin compounds were the significant contributors to the flavor. The types of the key flavor substances were the least (5 types only) after 7 months of storage. The research indicated that D-limonene was the main volatile component before and after pickling of Jiuzhi tangerine peel. The types and contents of volatile flavor substances changed to a certain extent during the pickling and storage.
    33  Comparison of the Quality and Aroma Components of Rizhao Green Tea harvested in Summer Dried under Different Roasting Conditions
    LI Wen-cui GAO Hua-feng FAN Qi-ye WANG Jia-peng TANG Xiao-lin
    2020, 36(11):255-262. DOI: 10.13982/j.mfst.1673-9078.2020.11.0495
    [Abstract](293) [HTML](204) [PDF 12.01 M](773)
    Abstract:
    In order to improve the overall quality of Rizhao green tea harvested in summer, the sensory evaluation, changes of main quality components and aroma components of Rizhao green tea harvested in summer dried by the technology of circular roll-roasting (processing for 30 minutes) and tea re-drying (processing for 30 minutes, 60 minutes and 90 minutes) were analyzed. The results showed that the quality characteristics of Rizhao green tea harvested in summer dried by different roasting methods were obvious different. The quality of green tea dried by re-drying technology (processing for 30 minutes) was better than other three roasting methods, with the tighter shape, the more moist color, the greener soup, no obvious water tightness taste; the higher retention of the contents (water extracts, tea polyphenols and free amino acids were 44.70%, 16.00% and 2.20%, respectively), the lower damage to the total amount of chlorophyll (0.27%), the significant reduction of heterocyclic compounds with high-fire aroma. Thirty-eight identical aroma components were detected in 4 kinds of Rizhao green tea dried by different methods. The main aroma components of green tea processed by there-drying technology for 30 minutes were esters, while the other three kinds of green tea were acids. Therefore, the problem of poor quality of traditional summer green tea could be effectively solved using the tea re-drying technology for 30 minutes at 130 ℃. The results of this work can provide theoretical basis for the application of there-drying technology in the processing of Rizhao Green Tea.
    34  Comparison of Quality Characteristics and Volatile Flavor Compounds of Pork Slices at Different Post-slaughter Time
    GAO Zi-wu WANG Heng-peng CHEN Sheng-shu WU Peng TU Ming-liang MENG Xiang-ren
    2020, 36(11):263-272. DOI: 10.13982/j.mfst.1673-9078.2020.11.0525
    [Abstract](265) [HTML](289) [PDF 4.27 M](676)
    Abstract:
    In order to investigate the effect of post-slaughter time on the quality characteristics and volatile flavor compounds of pork slices, the pork slices were starched at 12, 24, 36 and 48 h after slaughter, respectively, and the cooking loss rate, pH, color difference, moisture, protein, fat content, texture properties, microstructure and volatile flavor components were detected before and after the treatment. The results showed that the cooking loss rate of pork slices was significantly affected by the post-slaughter time (p<0.05). The cooking loss of pork slices was the lowest (8.14%) after 24 h slaughter. The pH of processed pork slices decreased continuously at the beginning of slaughter and began to rise after 36 h slaughter. The processed pork slices produced after 24 and 36 h slaughter showed good quality that the characteristics of color, nutrition, texture, brightness (L*) value, moisture content, elasticity and chewiness were acceptable. Results of microstructure showed that after 24 h slaughter, the gap of muscle bundle was bigger, the muscle fiber structure was looser and the tenderness was the best. Seventy-one kinds of volatile flavor compounds were detected before and after conditioning pork slices with different postmortem time, and the relative contents of aldehydes were 76.43% and 74.27% respectively after 24 and 36 h slaughter. The results showed that the pork from after 24 to 36 h slaughter was more suitable for the processing of prepared pork slices.
    35  Visual Semi-quantitative Detection of Hg2+ in Camellia Oil Using a Cadmium Telluride Quantum Dot-based Ratiometric Fluorescence Sensing System
    WANG Mei WEN Zhi-gang ZENG Xiu-ying YANG Fan PAN Lei YANG Liang ZHANG Shu-dong JANG Chang-long WANG Zhen-yang
    2020, 36(11):273-280. DOI: 10.13982/j.mfst.1673-9078.2020.11.0267
    [Abstract](214) [HTML](153) [PDF 82.78 M](732)
    Abstract:
    A simple ratiometric fluorescence system was designed for rapid, sensitive and visual detection of the Hg2+ content in camellia oil. The system was composed of cadmium telluride quantum dots (Cd/Te QDs) with red fluorescence and camellia oil naturally with blue fluorescence, and can detect and analyze the Hg2+ content in camellia oil (as low as 6.04 nM). Appropriate adjustment of fluorescence intensity ratio made the fluorescence of the sensor system turn pink. The red fluorescence of CdTe QDs in the system was quenched due to the charge transfer upon the addition of Hg2+. With the increase of the Hg2+ concentration, the color of the system exhibited significant color change (from pink color to blue color). In order to evaluate the selectivity of the ratio fluorescence system towards Hg2+, different metal ions were added to the ratiometric fluorescence system under the same conditions did not change the fluorescence intensity and color of the system. Further addition of 200 nM of Hg2+ decreased greatly the fluorescence intensity ratio (I677/I431) of the system and changed significantly the fluorescence color. These results indicated that thus this system exhibited strong anti-interference ability and could meet the detection requirements of actual samples. The simple method is fast, sensitive and inexpensive, and provides a new strategy for rapid detection of heavy metal ions in camellia oil.
    36  Detection and Dietary Risk Assessment of Several Heavy Metals in Rice from Different Origin in Heilongjiang Province
    ZHANG Chang REN Xiao-yu CUI Hang LIANG Meng-ting CAO Dong-mei
    2020, 36(11):281-286. DOI: 10.13982/j.mfst.1673-9078.2020.11.0489
    [Abstract](313) [HTML](275) [PDF 714.41 K](839)
    Abstract:
    In this paper, inductively coupled plasma mass spectrometry (ICP-MS) was used to analyze cadmium, lead, chromium and arsenic in rice which were collected from five major rice producing areas of Heilongjiang (Chahayang, Wuchang, Fangzheng, Xiangshui and Jiansanjiang). The contents of the four elements (cadmium, lead, chromium, and arsenic) were tested and analyzed, and the exposure to four elements in rice was evaluated by monthly intake value (EMI) and lead exposure boundary ratio (MOE) for the residents in this area. The results showed that the measured average contents of four element (cadmium, lead, chromium, and arsenic) were 0.85×10-2 mg/kg, 0.40×10-2 mg/kg, 1.07×10-1 mg/kg and 1.14×10-1 mg/kg, respectively, and the coefficient of variation range is 0.32~2.53, which is within the national standard. The exposure boundary ratio of lead in the population of Heilongjiang province was 45.06~982.48; the monthly intake of cadmium, chromium and arsenic were measured to be 0.17~3.22 μg/kg·BW, 11.77~26.49 μg/kg·BW and 22.596~31.394 μg/kg·BW, accounting for 0.6%~12.8%, 46.7%~92.2%, 1.5% ~ 2.1% of the JECFA prescribed limits respectively, which did not exceed the JECFA prescribed limits. The content of cadmium, lead, arsenic and chromium in rice in the five major producing areas of Heilongjiang province and the population intake are generally at a safe level.
    37  Pollution Characteristics and Health Risk Assessment of Phthalates in Melon Field Soil
    Mahebali•Tuohutaerhan ZHANG Yan-ming SHEN Qi Guliziya•Tuersibieke HE Wei-zhong KONG Ling-ming WANG Cheng
    2020, 36(11):287-295. DOI: 10.13982/j.mfst.1673-9078.2020.11.0478
    [Abstract](328) [HTML](192) [PDF 6.33 M](1043)
    Abstract:
    In order to assess the pollution characteristics of Phthalate esters (PAEs) in the melon fields of Xinjiang and their risks to human health, 33 surface soil samples were collected in the assessment area and detected by gas chromatography-mass spectrometry. The content and main components of the 11 PAEs compounds detected in the soil were analyzed, compared with the control and treatment standards for PAEs in the soil, and the five types of detection were tested by the model recommended by the US Environmental Protection Agency (US EPA), and PAEs with priority control were evaluated for human health risks. The test results showed that total of 11 PAEs compounds were detected in the melon field soil in the assessment area, the content ranging from 57.30 to 3272.70 μg/kg, with the average value of 253.10 μg/kg; bis-iso-butyl ester (DIBP), bis-ethyl ester (DEP) andbis-2-ethylhexyl ester (DEHP) are the main pollutants, accounting for more than 90% of the total content of PAEs compounds; DEP contents in 6 soil samples exceed the control standard (not exceeding the governance standard), the rate of exceeding the standard is 18%, the remaining samples does not exceed the control standard and the governance standard; priority control of the non-carcinogenic risk (HQ) value of the compound to the human body is less than 1.0. Both benzylbutyl ester (BBP) and DEHP carcinogenic risk (CR) values are less than 1.0×10-6, within the acceptable range. Based on the above analysed results, it can be concluded that the PAEs pollution phenomenon in the soil samples of the melon fields in the assessment area is relatively low compared with the PAEs content of soils in otherurban facilities. The soil PAEs content in this study is relatively low, and its pollution degree, carcinogenic risk and non-carcinogenic risks are within the acceptable range.
    38  Research Progress on Detection Methods for Perfluorooctane Sulfonic Acid (PFOS) in Food Contact Materials
    NIU Bing XU Si-yan ZHAO Chao-min DENG Xiao-jun
    2020, 36(11):296-304. DOI: 10.13982/j.mfst.1673-9078.2020.11.0418
    [Abstract](444) [HTML](348) [PDF 2.65 M](918)
    Abstract:
    Perfluorooctane sulfonic acid (PFOS) has attracted more and more attention from scholars at home and abroad in recent years due to its ability to bioaccumulate, toxicity and degradation-resistance. As a typical perfluorinated compound widely distributed, PFOS contaminates many daily foods in various ways, such as food contamination through fast food packaging/wrappers, beverage contamination through disposable cardboard cups, food contamination through nonstick frying pan coating, in particular, the contamination through food contact materials is the most neglected pathway. PFOS has a variety of toxic effects including acute toxicity, hepatotoxicity, neurotoxicity, immunotoxicity, cytotoxicity and reproductive toxicity. However, the content of PFOS in the contaminated food contact materials is often relatively low. Thus, rapid and accurate determination of PFOS is challenging. In this paper, the toxicity of PFOS, contamination levels of PFOS in various media, migration of PFOS to foods, and various methods for detecting PFOS in food contact materials were reviewed. The detection of PFOS in food contact materials and associated developments are summarized and future outlook is presented.
    39  Flavor Contribution of Lactones in Cheese and Its Biosynthetic Regulation: a Review
    CHEN Chen LIU Zheng YU Hai-yan TIAN Huai-xiang
    2020, 36(11):305-312. DOI: 10.13982/j.mfst.1673-9078.2020.11.0513
    [Abstract](241) [HTML](277) [PDF 7.25 M](1485)
    Abstract:
    The lactones in cheeses mainly include γ-lactones and δ-lactones, which are one kind of the key odorants of cheese and make key contributions to the formation of cheese flavor. In this paper, a systematic review of the composition, flavor contribution and concentration range of major lactones in common cheeses was discussed based on consulting relevant literature. The six main quantitative methods for lactones including GC external standard method, peak area normalization method, ultra performance liquid chromatography-mass/mass spectrometry, GC-MS internal standard method, selective ion flow tube mass spectrometry, stable isotope dilution analysis were introduced. The formation mechanism of lactones and the biosynthetic control strategies proposed by researchers were summarized. The current issues and future research directions were also forecasted. A theoretical basis was provided to fully understand the lactones and their formation and regulation in cheeses. This review will be important for the development of original cheeses which is more suitable for Chinese consumers and the transformation and upgrading of the cheese industry in China.

    Editor in chief:李琳

    Inauguration:现代食品科技

    International standard number:ISSN 1673-9078

    Unified domestic issue:CN 44-1620/TS

    Domestic postal code:46-349

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