Volume 0,Issue 6,2020 Table of Contents

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  • 1  Protective Effect of Houttuynia cordata Polysaccharide on LPS Induced Chronic Inflammatory Lung Injury in Rats
    SONG Ye-hao YOU Hui-ting YAO Yu-fei WU Rui-ting FU Wang-wei WAN Min LI Wen-juan
    2020, 36(6):1-8. DOI: 10.13982/j.mfst.1673-9078.2020.6.1291
    [Abstract](382) [HTML](340) [PDF 952.38 K](684)
    Abstract:
    The effect of Houttuynia cordata polysaccharide (HCP) on LPS induced chronic inflammatory lung injury was investigated in the present work. The chronic inflammatory model was established via the intravenous injection of LPS, and the number of WBC, monocyte and the content of serum hypersensitive C-reactive protein (hs-CRP) were measured in this work. Furthermore, the pathological changes of lung tissue were observed. The contents of antioxidant enzyme, malondialdehyde (MDA), nitric oxide (NO), nitric oxide synthase and the level of cytokines in lung tissue were determined. The results showed that, the content of WBC, monocyte and serum hs-CRP in high dose treatment of HCP group were significantly reduced by 17.89%, 38.37% and 35.61% respectively, when compared to LPS group. Moreover, pathological observation showed that HCP treatment group could alleviate significantly the pathological state of pulmonary edema. Meanwhile, the activity of antioxidant enzyme in lung tissue of HCP treatment group was significantly increased. Strikingly, the content of MDA, NO, iNOS, tNOS and the cytokine levels of IL-6, IL-1β, TNF-α in high dose treatment of HCP group were remarkably reduced by 28.79%, 22.88%, 37.06%, 27.49%, 34.19%, 38.84% and 52.27%, respectively. In conclusion, the protective effects of HCP against chronic inflammatory lung injury in rats was linked to its activities on the reduction of inflammatory cell secretion, the alleviation of pulmonary edema and other pathological states, the inhibition of oxidative stress, and the reduction of the release of inflammatory factors.
    2  Chicken Enzymatic Hydrolysates Improved Anti-fatigue and Antioxidant Activities in Mice
    ZHENG Hua MO Ni-mei DAI Yan LIN Jie LI Hang-yu ZHENG Xia-tong WU Wei-bin
    2020, 36(6):9-16. DOI: 10.13982/j.mfst.1673-9078.2020.6.1079
    [Abstract](422) [HTML](342) [PDF 771.36 K](738)
    Abstract:
    This study investigated the anti-fatigue and anti-oxidation effects of chicken enzymatic hydrolysates EP1 and EP2. Normal saline and chicken homogenate [protein dose 200 mg/(kg·bw·d)] were used as the control group, and two doses of EP1 and EP2, 40 mg/(kg·bw·d) and 200 mg/(kg·bw·d), were administered by gavage into the mice. After 28 days of administration, the exhausted swimming time, and the serum urea nitrogen content, serum lactic acid content and liver glycogen content in the mice after 30 min of swimming were measured. The activities of SOD (superoxide dismutase), GSH-Px. (glutathione peroxidase) and CAT (catalase) in mouse serum were also analyzed. The results showed that chicken hydrolysates could effectively prolong the exhausted swimming time of mice (the SC group 766 s; the H-EP1 group 2307 s). After 30 min of swimming, the contents of serum lactic acid (the SC group 10.06 mmol/L; both the H-EP1 and H-EP2 groups 6.66 mmol/L) and serum urea nitrogen (the SC group 19.72 mmol/L; the H-EP1 group 13.34 mmol/L) decreased significantly, whilst the content of liver glycogen (the SC group 5.11 mg/g; the H-EP2 group 8.35 mg/g) as well as the activities of serum SOD, GSH-Px. and CAT antioxidant enzymes increased significantly. The research results suggest that chicken enzymatic hydrolysates EP1 and EP2 could significantly extend the exhaustion swimming time of mice, reduced the consumption of liver glycogen and the production and deposition of lactic acid in mice after intense exercise, promoted protein metabolism, removed effectively the serum urea nitrogen in mice, increased the antioxidant activities of serum SOD (from 89.08 to160.13 U/mL), CAT (from 0.25 to 0.29 U/mL) and GSH-Px. (from 257.36 to 597.08 U/mL) in mice, enhance the free radical scavenging ability of mice, and improved the exercise status of mice. These changes ensure that the mice subjected to intense exercise remained in the mode of aerobic exercise, so as to reduce the body’s fatigue after intense exercise, with significant anti-fatigue and anti-oxidation effects.
    3  Anti-inflammatory Mechanism Research of Astragalin on Rat Intestinal Epithelial Cell (IEC-6) Inflammation Model Induced by LPS
    NIE Long PENG Lei ZHANG Hao LI Yu-fang TIAN Yang
    2020, 36(6):17-23. DOI: 10.13982/j.mfst.1673-9078.2020.6.1201
    [Abstract](616) [HTML](274) [PDF 866.82 K](1056)
    Abstract:
    This study investigated the anti-inflammatory effect of Astragaloside on rat intestinal epithelial cells (IEC-6) model induced by lipopolysaccharide (LPS). A model of IEC-6 cell inflammation induced by LPS was established, and the survival rate, the related inflammatory factors, genes and protein expression levels of inflammatory IEC-6 cells were determined by MTT method, Enzyme-linked immunosorbent assay (ELISA), real-time fluorescence quantitative PCR and Western-blot, respectively. The results showed that the survival rate of IEC-6 cells increased gradually with the increase of Astragalin concentration. The survival rates of IEC-6 cells in different dose groups (50 μg/mL and 100 μg/mL) were 90.68% and 95.76% (p<0.05 and p<0.01), respectively. Enzyme-linked immunosorbent assay (ELISA) showed that compared with the LPS group, 50 μg/mL and 100 μg/mL of Astragalin significantly inhibited the secretion level of inflammatory factor IL-6, with inhibition rates of 21.98% (p<0.05) and 29.05% (p<0.01), respectively. The mRNA expression level of IL-6 was decreased by 34.90% (p<0.05) and 41.60% (p<0.01), respectively.The inhibitory rates of TNF-α secretion were 16.25% (p<0.05) and 23.37% (p<0.01), respectively, and the mRNA expression levels were decreased by 34.11% (p<0.05) and 43.84% (p<0.01), respectively. In terms of protein pathway, 100 g/mL of Astragalin showed significant inhibition of P-IKKα/β level 27.46% (p<0.05) and P-IκBα level 41.52% (p<0.05) in NF-κB pathway induced by LPS. This study provides a reliable basis for the development of Astragalin as a potential drug to relieve inflammatory bowel disease.
    4  Inhibition Activity of Oral Cancer KB Cell of Chondroitin Sulfate from Different Sources
    ZHANG Yang-xing WANG Lan XIONG Guang-quan WU Wen-jin SHI Liu QIAO Yu WANG Jun
    2020, 36(6):24-32. DOI: 10.13982/j.mfst.1673-9078.2020.6.1069
    [Abstract](416) [HTML](206) [PDF 947.30 K](687)
    Abstract:
    Chondroitin sulfate was extracted from the sturgeon cartilage and chicken cartilage using the alkaline extraction-enzymatic-alcohol precipitation process, and purified by Sephadex G-50 gel chromatography and Sephacryl S-300 HR gel chromatography. The molecular mass and purity of the obtained chondroitin sulfate were determined by HPGPC. The effect of chondroitin sulfate on the proliferation of oral cancer KB cells was detected by the MTT assay, and the apoptosis of oral cancer KB cells was examined by flow cytometry. The results showed that the yield of crude chondroitin sulfate from sturgeon cartilage and chicken cartilage was 23.80% and 25.23%, respectively. HPGPC analysis showed that the purified chondrocytes from sturgeon cartilage and chicken cartilage were highly pure polysaccharides, with the average molecular weight of the chondroitin sulfate in sturgeon cartilage higher than that in chicken cartilage. The inhibitory effects of crude and purified chondroitin sulfate on oral cancer KB cells were studied in vitro. The results showed that the IC50 values were 80.18 μg/mL and 58.78 μg/mL, respectively, for the crude and purified chondroitin sulfate from sturgeon cartilage, and 68.55 μg/mL and 53.71 μg/mL, respectively, for the crude and purified chondroitin sulfate from chicken cartilage. Flow cytometry showed that chondroitin sulfate could induce the apoptosis of KB cells, and the apoptosis rate was dependent on the concentration of the chondroitin sulfate. The apoptosis rate induced by the chondroitin sulfate in chicken cartilage was higher than that from sturgeon cartilage.
    5  In vitro Antioxidant Activity and Reduction of Postprandial Blood Glucose by the Extracts from Sargassum fusiforme
    ZHENG Xiao-li YAN Li-ping ZUO Ji-hui CHEN Ling CHENG Yang WU Ming-jiang TONG Hai-bin
    2020, 36(6):33-39. DOI: 10.13982/j.mfst.1673-9078.2020.6.1155
    [Abstract](366) [HTML](270) [PDF 898.00 K](671)
    Abstract:
    To study the antioxidant and lower postprandial blood glucose activities by different solvents extracts from Sargassum fusiform, the ethanol extract of Sargassum fusiforme was fractionated by different organic solvents (Petroleum ether, dichloromethane, ethyl acetate, butyl alcohol, PE, DCM, EAC, NBA). The contents of phenols and flavonoids were measured, then the DPPH·, ABTS·, and FRAP methods were used to evaluate the antioxidant activity, the p-nitrophenyl-α-D glucopyranoside (PNPG) and DNS methods were used to determine the inhibition rate of α-glucosidase and α-amylase. Finally, glucose tolerance was measured to evaluate the effect on the postprandial blood glucose. Result showed that EAC extract exhibited the highest total phenols and flavonoids contents, which were 34.47 mg/g and 5.77 mg/g, respectively, and its antioxidant activity was only slightly lower than that of NBA extract in DPPH test, when the concentration was 1.6 mg/mL, the antioxidant activities of EAC extract were 90.17% (DPPH·), 0.35 mmol/mg (ABTS·) and 0.72 mmol/mg (FRAP); in the α-glucosidase inhibition experiment, the IC50 of PE, DCM, EAC extracts were 0.54, 0.29, and 0.27 mg/mL, respectively. The first three extracts can significantly reduce postprandial blood glucose. This study shows that EAC extract has the potential to be used as a dietary supplement or anti-diabetic medicine, providing a theoretical basis for the development and utilization of Sargassum fusiforme.
    6  The Synthesis Dynamics of Carotenoids and Antioxidant Activity in Different Parts of Maize Kernel during Germination
    BAO Yi-hong LUO Hao HE Wei-wei WANG Yu-xi ZHOU Zhi-yi LI Da-jing
    2020, 36(6):40-45. DOI: 10.13982/j.mfst.1673-9078.2020.6.1099
    [Abstract](371) [HTML](277) [PDF 750.96 K](497)
    Abstract:
    Yellow maize kernels were used as the raw materials in this study. The synthesis dynamics of total carotenoids, lutein and zeaxanthin as well as the antioxidant activity in embryos, germs and radicles were investigated during germination. The results showed that the germination rate of corn kernels increased with the germination time, and the germination time of corn kernels was between the 12th and 36th h. During germination, the fresh weight of embryo, radicle and germ showed an upward trend, following the decreasing order of the embryo, followed by embryo>germ>radicle. With the increase of germination time, the total carotenoids accumulated in the embryo and radicle increased, whilst the total carotenoid accumulation in the germ increased significantly at 36 th~48 th h and 60 th~72 th h. The main carotenoid in the embryo, radicle and germ was lutein, and the total carotenoid content in the germ was significantly higher than those in the radicle and embryo. The total carotenoid content of corn kernels after germination for 72 h was 6.19 times that before germination. At the same time, the antioxidant activity in germ and embryo increased with the germination time, with the highest reaching 8.01 μmol TEAC/g and 8.51 μmol TEAC/g, respectively. However, an opposite trend was for the radicle. Therefore, germination can significantly increase the carotenoid content in different parts of the maize kernels and the antioxidant activity of the germ.
    7  Comparative Analysis of the Structure and Gelation of Egg White Protein under Different Pressure Conditions
    LYU Jing LIU Ya-ping DANG Run-qing JIN Yong-guo MA Mei-hu
    2020, 36(6):46-53. DOI: 10.13982/j.mfst.1673-9078.2020.6.1208
    [Abstract](207) [HTML](348) [PDF 979.27 K](1020)
    Abstract:
    For traditional egg products, marinated egg, salted eggs, and preserved eggs usually require a long processing time. To improve processing efficiency and product quality, three processing methods were selected: atmospheric pressure, high pressure, and pulsed pressure in this study. Under salt-free and salt-containing conditions, the salt content of egg whites, surface hydrophobicity, changes in primary and secondary structure, and gel characteristics of egg white proteins were compared and analyzed. It was found that the salt content of egg white after pulsed pressure treatment was 2.10±0.23 times of that of the untreated; The combined effect of pressure, temperature and salt increased the surface hydrophobicity of egg white proteins, the wavelength of amide II band red-shifted to 1520 cm-1 after pulsating pressure treatment, the content of α-helix and random curl decreased, and the content of β-sheet increased; NaCl treatment can reduce the gelability of egg whites, Compared with high pressure treatment, pulsed pressure can relatively improve the adverse effects of NaCl; The Springiness of the egg white gel after pulsed pressure treatment was increased to 1.02, the T21 relaxation time was reduced to 25.79 ms, and the microstructure was greatly improved. Therefore, the pulsed pressure can improve the processing efficiency and quality of egg products, and provide a certain reference for its application in production.
    8  Chemical composition and Antioxidant Activity of Styela plicata dominant in Beibu Gulf
    SUN Xue-ping TAN Xiao-ming QIN Xi-jun TAN Xiao-qing LIU Bu-ming
    2020, 36(6):54-59. DOI: 10.13982/j.mfst.1673-9078.2020.6.1024
    [Abstract](287) [HTML](324) [PDF 753.87 K](760)
    Abstract:
    The total antioxidative activity, and the scavenging abilities of DPPH, superoxide and hydroxyl free radicals of the different organic extracts from the dominant squirt Styela plicata of Beibu Gulf were evaluated for the first time by ultraviolet spectrophotometry. In the total reducing power experiments, the absorbance values of the ethyl acetate phase and water phase at the concentration of 100 μg/mL were 0.149 and 0.122, respectively. The DPPH· scavenging ability is greater in the water phase, with the IC50 value being 6.10 μg/mL. The IC50 values of the water phase and ethyl acetate phase for superoxide anion were 10.26 μg/mL and 2.18 μg/mL, respectively, with their corresponding IC50 values for hydroxyl free radical were 2.30 μg/mL and 9.65 μg/mL, respectively. The results showed that the active components of the squirt’s secondary metabolites possessing significant in vitro antioxidant activity were mainly concentrated in the aqueous phase and ethyl acetate phase. The ethyl acetate phase was subjected to further separation and purification by normal- and reversed-phase silica gel column chromatography, Sephadex LH-20 column chromatography and semi-preparative HPLC. The structures of active compounds were characterized by spectroscopic methods including NMR and MS, and three of which were identified as polyhydroxy sterols cholestane-1,3,5,6-tetraol(1), (5)-cholestane-3,5,6-triol(2) and suberoretisteroids D(3). These three compounds were obtained from S. plicata for the first time. The experimental results provide fundamental information and guidance for increasing the application value of S. plicata and also offers resources for the development of food-derived natural antioxidants.
    9  The Difference for Enzymatic Hydrolysis of Sugarcane Bagasse Pre-treated by Different Deep Eutectic Solvents
    HUANG Zi-jian YANG Lu-min LIN Kai-peng FENG Guo-jian ZHANG Yong-hao PU Fu-long GU Rong-rong HOU Xue-dan
    2020, 36(6):60-67. DOI: 10.13982/j.mfst.1673-9078.2020.6.1234
    [Abstract](343) [HTML](265) [PDF 770.94 K](634)
    Abstract:
    In order to evaluate the influence of deep eutectic solvents (DESs) pretreatment on lignocellulosic biomass, five DESs with choline chloride (ChCl) as hydrogen bond acceptor (HBA) and five DESs with malic acid as hydrogen bond donor (HBD) were used to pretreat sugarcane bagasse. The effects of HBDs and HBAs on the components and enzymatic hydrolysis efficiency of biomass were studied. Among ChCl-based DESs, lactic acid-choline chloride (LC) showed the best enzymatic hydrolysis efficiency. The cellulose content in the residue was 72.92%. The glucose yield and cellulose digestibility after saccharification were 56.90% and 60.43%, respectively. Among malic acid-based DESs, malic acid-choline chloride was the best one with the highest xylan and lignin removal, resulting in 43.35% of cellulose digestibility and 38.00% of glucose yield. The results showed that both HBD and HBA played a non-ignorable role in biomass deconstruction, and the strength of intermolecular hydrogen bond in DESs was negatively correlated with their deconstruction ability. Besides, it was found that the xylan removal could be correlated to the pKa value of HBDs or HBAs, while the cellulose digestibility was positively related to xylan removal, instead of delignification. In addition, the intermolecular hydrogen bond strength of DESs and the hydrogen bonding interaction between DES and biomass have been proved to be related to the pretreatment temperature and time in a certain range. Results of this work will provide a theoretical basis for developing novel DESs for biomass pretreatment.
    10  Structure and Property of Chitosan/Starch Composite Film with Different Kinds of Starch
    YANG Jing-wen LI Xiang-qi LI Shan-shan LI Jia-ying SITU Wen-bei
    2020, 36(6):68-74. DOI: 10.13982/j.mfst.1673-9078.2020.6.1244
    [Abstract](304) [HTML](257) [PDF 845.76 K](1136)
    Abstract:
    To improve the bioavailability of drug or bioactive component, in this work, composite films with 150,000 g/mol chitosan and three different kinds of starch were prepared by casting method. The characteristics were analyzed by FTIR, SEM, tensile test and in vitro releasing test. By the hydrogen bond between -NH3+ of chitosan and -OH of starch, there was formed chitosan/starch composite structure in the film. Due to difference in starch structure, the order of chitosan/starch composite structure was changed, which further affected the mechanical properties, acid resistance and controlled-releasing properties of the film. Results showed that the order of CTS/Potato and CTS/Tapioca composite film structure was better than that of CTS/Corn. The CTS/Potato and CTS/Tapioca composite film also showed a good acid resistance in simulated stomach fluid (film loss at the range of 18.71%~26.15% and 16.14%~19.64%, respectively) and a well-controlled-releasing property of theirs film-coated tablet (about 60% of the model bioactive component releasing in small intestine or colon), which could be potential to bioactive component for small intestine or colon site delivery.
    11  Comparative Analysis on Volatile Components and Biological Activities of Different Garlic Essential Oil
    WU Yan HE Yuan-qin YI Jun-ming SHEN Li-qun
    2020, 36(6):75-81. DOI: 10.13982/j.mfst.1673-9078.2020.6.1214
    [Abstract](316) [HTML](297) [PDF 689.23 K](894)
    Abstract:
    In order to understand the difference of composition and biological activities of the essential oils from white skin garlic and purple skin garlic, the essential oils were obtained by using steam distillation method, and their volatile components were analyzed and identified by gas chromatography-mass spectrometry (GC-MS). The inhibitory activities of purple skin and white skin Garlic Essential oils on several common pathogenic bacteria were tested by dilution doubling method. The antifeedant activity of the oils against Spodoptera litura was measured by leaf disc method. The antioxidant activities of two kinds of garlic were evaluated by ABTS, DPPH and hydroxyl free radical scavenging. The results showed that 26 components were identified in the essential oil of white garlic, accounting for 95.79% of the essential oil; 22 components were identified in the essential oil of Purple Garlic, accounting for 94.26% of the essential oil; the main bactericides, allicin and allicin, were more in the purple garlic (59.34%) than in the white garlic (50.11%); the two essential oil of garlic had obvious inhibitory effect on the experimental bacteria, especially the essential oil of Purple Garlic The results showed that the inhibition effect of Bacillus, Candida albicans, Candida tropicalis and chestnut blight was better than that of Allium bungeanum; the average antifeeding rate of Allium bungeanum and Allium bungeanum essential oil to Spodoptera litura for 24 hours was 52.97% and 100% respectively; the scavenging rate of ABTS and DPPH free radicals by Allium bungeanum essential oil was higher than that by Allium bungeanum essential oil, and the scavenging rate of hydroxyl free radicals was basically the same.
    12  Structures and Property Characterization of Starch-chitosan Crosslinking Films with Different Amylose/amylopectin Ratio
    ZHANG Shu-yan HOU Fei-xiang MAI Jun-xi XIAN Wei-xiong ZHU Jie LI Lin
    2020, 36(6):82-88. DOI: 10.13982/j.mfst.1673-9078.2020.6.1219
    [Abstract](442) [HTML](254) [PDF 886.87 K](792)
    Abstract:
    Starch-chitosan crosslinking films were prepared by starch with different amylose/amylopectin ratios (waxy, normal, G50 and G80). Scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction technology (XRD), Contact angle meter (CA) and Thermal gravimetric analysis (TGA) were applied to characterize the morphology, multi-scale structures and thermal property discrepancies of starch-chitosan crosslinking films. The results showed that cavities were formed in the film prepared by the self-cross linkage of chitosan. When chitosan was crosslinked with starch, holes disappeared and homogenous fractured surface were newly formed in the film, and with the increase of amylose content, the roughness of cross-linked film was more obvious. In the meanwhile, new hydrogen-bonds were newly formed by crosslinking of chitosan and starch, the weak diffraction peaks around at 2θ=17.36° of G50 and G80 self-crosslinking films disappeared, and the interactions promoted amorphous structures formation in all chitosan-starch crosslinking films. The contact angle of chitosan self-crosslinking film was 76.57°±1.35°, when chitosan was crosslinked with starch with different amylose/amylopectin ratio, the contact angles of all starch-chitosan crosslinking films were increased, and films with higher amylopectin content showed stronger hydrophilia. The cross-linkage of chitosan and starch accelerated the depolymerization and decomposition of macromolecules, leading to lower thermal stabilities compared with the films by self-crosslinking of starch and chitosan. The results could provide reasonable utilization of starch-chitosan crosslinking film in the field of food packaging.
    13  Stability and Fluorescence Characteristics of C-phycocyanin in Spirulina platensis
    LIANG Xiao WANG Cheng-hua LU Xin PANG Yi-yang JIANG Hong-rui LIU Xiao-ling
    2020, 36(6):89-96. DOI: 10.13982/j.mfst.1673-9078.2020.6.1233
    [Abstract](424) [HTML](427) [PDF 937.44 K](1369)
    Abstract:
    In this study, the spectral characteristics of C-phycocyanin (C-PC) from Spirulina platensis were studied, and the changes of its solution stability and fluorescence characteristics under the environmental factors such as temperature, pH, light (intensity, time, light source), protein denaturants. The results showed that the color stability and fluorescence characteristics of C-PC were affected by factors such as pH, light, temperature, and protein denaturants. Under the conditions of dark, pH 6~7, and temperature ranged 4~30 ℃, the pigment retention rate and blue value b* of C-PC solution were less than 4.93%, which has good stability. C-PC was sensitive to light, of which the red and blue band light sources have the greatest influence (p<0.05). In the solution of protein denaturants, certain concentrations of guanidine hydrochloride (≥2 mol/L), ethanol (≥50%, V/V), and sodium lauryl sulfate SDS (≥0.05%, m/V) could make C-PC seriously unstable and discolored, and quenched its fluorescence peak. Correlation analysis showed that the color change of C-PC caused by temperature and light intensity was significantly correlated with the peak of its fluorescence characteristics (p<0.05). However, the color change caused by pH or protein denaturants has no significant correlation with the peak of its fluorescence characteristics (p>0.05), indicating that the fluorescence characteristics of C-PC were not completely consistent with the changes in its color stability. The results provided references for the application of C-PC in natural pigments, fluorescent reagents or further research on the mechanism of instability.
    14  Comparative Analysis of Different Sterilization Ways on Quality of Prepared Carrot Slices
    LIU Jun-mei MI Rui-fang HU Xiao-song WU Ji-hong
    2020, 36(6):97-104. DOI: 10.13982/j.mfst.1673-9078.2020.6.1269
    [Abstract](368) [HTML](292) [PDF 753.13 K](625)
    Abstract:
    The purpose of this work was to study the effects of three different sterilization methods on the quality of prepared carrot slices from raw materials during cold storage at 4°C. The samples were pretreated by pasteurization (90°C, 30 min), high hydrostatic pressure (HHP 550 MPa, 25°C), pressure-assisted thermal sterilization (PATS 550 MPa, 50°C) and stored at 4°C for 60 d. The control group was unsterilized carrot slices. Smartouge combined with sensory evaluation were used to determine the differences among different samples during storage. Results showed that principal component analysis (PCA) and linear discriminant analysis (LDA) techniques combining with smartongue could distinguish carrot slices treated with different sterilization methods. Quality differences could be observed from the PCA and LDA figures in shelf life of 60 d. The quality of HHP sterilized carrot slices was close to that of unsterilized samples’ in 15 days. Results of sensory evaluation showed that the carrot slices treated with high hydrostatic pressure were similar to control samples in color, smell, taste and shape. In conclusion, comparing with the carrot slices samples treated by different sterilization processing, HHP sterilizated carrot slices had best quality, and was close to the control group within 15 days of storage.
    15  Quality of Spinach-fortified Fresh Noodles under Different Storage Conditions
    WEN Qing-yu ZHANG Kang-yi ZHAO Di SUN Jun-geng GAO Ling-ling GUO Dong-xu JIN Chang-chun
    2020, 36(6):105-113. DOI: 10.13982/j.mfst.1673-9078.2020.6.1086
    [Abstract](373) [HTML](187) [PDF 771.86 K](656)
    Abstract:
    Taking spinach-fortified fresh noodles as the research object, and the water content, water activity, pH, acidity, color, chlorophyll content, sensory attributes, and colony counts as assessment indicators, the effects of three storage methods (lighting, darkness and CO2) on product quality were studied at 4 ℃ and 25 ℃. The experimental results showed that the water content, water activity, pH, color, chlorophyll content and sensory attributes of the spinach-fortified fresh noodles declined gradually, while the acidity and colony count increasing gradually, with the prolongation of storage time at 4 ℃ and 25 ℃ under the three storage conditions. The products quality was the best under the storage at 4 ℃ and with CO2. The sensory score and the total number of colonies were 7.1 and 8.7×104 CFU/g, respectively, after the storage for 5 days (the turning point of product quality). The sensory quality and the total number of colonies of the spinach-fortified fresh noodles were correlated to the moisture content, acidity, water activity, pH, total chlorophyll content, chlorophyll a content, chlorophyll b content, L* value, a* value, b* value of the product. This study provides important data support for extending the shelf life and breakthrough in technical bottleneck restricting industrial development through investigating storage quality under different storage conditions.
    16  Screening of Molds in Traditional Soy Sauce Moromi and Analysis of Some Enzymatic Characteristics
    ZHAO Mou-ming LIN Han-yu LIANG Zhuo-xiong LU Huai-jin XIE Nuo-yi FENG Yun-zi
    2020, 36(6):114-120. DOI: 10.13982/j.mfst.1673-9078.2020.6.0829
    [Abstract](360) [HTML](492) [PDF 774.80 K](1047)
    Abstract:
    There are many types of microorganisms (most of which naturally occurring in the environment) participating in the fermentation of traditional soy sauce. The aim of this study was to isolate different molds from the traditional soy sauce moromi and examine their enzymatic activities. Forty-nine mold strains were isolated from moromi, among which 18 cultured mold strains with different morphologies were selected for molecular biological identification. The results revealed four genera, including Aspergillus, Penicillium, Cladosporium and Alternaria. Analysis of enzymes in these molds showed that the enzymatic characteristics were closely related to the mold species. Among which, the acid protease and β-glucosidase of Aspergillus niger (A. niger) were prominent, while neutral protease, glucoamylase and aminopeptidase of Aspergillus oryzae (A. oryzae), Aspergillus nomius and Alternaria alternate were prominent. According to enzymatic activity and strain characteristics, A. niger (AN1) and A. niger (AN2) were chosen to co-cultivate respectively with commercial A. oryzae to make mixed koji. It was found that the effect of the mixed koji with A. niger AN2 and A. oryzae was better. Compared with pure A. oryzae koji, the activities of neutral protease and acid protease in mixed koji increased by 42.84% and 22.27%, respectively, and the activities of glucoamylase and aminopeptidase also increased by 17.54% and 15.10%, respectively, indicating a high application potential. This study provides a theoretical basis for screening high-quality mold from moromi.
    17  Cloning and Expression Analysis of Versatile Peroxidase from Pleurotus tuber-regium
    TIAN Yun-heng YANG Jia-liang MA Ai-min
    2020, 36(6):121-127. DOI: 10.13982/j.mfst.1673-9078.2020.6.1127
    [Abstract](397) [HTML](189) [PDF 851.35 K](788)
    Abstract:
    Primers were designed according to the conserved domain of versatile peroxidase gene cDNA sequences of white-rot fungi, and the gene of versatile peroxidase from Pleurotus tuber-regium (termed Pt-vp1; GenBank accession number: MN701764) was obtained via amplification using degenerate PCR, rapid amplification of cDNA end (RACE), and fusion primer and nested integrated PCR (FPNI-PCR). The full-length DNA of Pt-vp1 was 1833 bp, with 15 exons and 14 introns. The length of cDNA and open reading frame were both 1077 bp and encoded 358 amino acids. Phylogenetic analysis of the amino acid sequences of VPs from various white-rot fungi showed that Pt-vp1 protein was the closest to Pleurotus ostreatus VP (GenBank accession number: ASU87523.1). The VP activity of P. tuber-regium reached the highest to 43.83 U/L after 4 days of stationary culture in the fermentation medium. Mn2+ was added at different concentrations. When its concentration was 200 μmol/L, the VP activity was the highest 91.98 U/L. The results indicated that Mn2+ was not the necessary factor for producing VP in Pleurotus tuber-regium, but could increase significantly the enzyme production. The effect of Mn2+ at different concentrations on the transcription level of Pt-vp1 was examined using quantitative real-time PCR (qRT-PCR), and the results showed that Mn2+ promoted the expression of Pt-vp1. When the concentration of Mn2+ was 300 μmol/L, the transcription level of Pt-vp1 increased 5.79 times. This study provides important information for investigating the expression mechanism and function of the VP gene from P. tuber-regium.
    18  Metagenomics Analyze the Relationship between the Dominant Bacteria and Fungi and Metabolism Function in the Pit Fermentation of Maotai-flavor Liquor
    MA Ying-yao HU Ping SUN Li-lin TIAN Ya
    2020, 36(6):128-136. DOI: 10.13982/j.mfst.1673-9078.2020.6.1251
    [Abstract](421) [HTML](299) [PDF 896.91 K](893)
    Abstract:
    In this work, metagenomics technology was used to study and analyze the dominant bacteria and fungi communities change, metabolic function difference and correlation of fermented grains in the fourth round of Maotai-flavor liquor cellar. The results showed that the pit fermentation grains were in acid environment, the content of alcohol and acid in 30 days fermentation were higher than that of 0 days fermentation, and the types of esters were more abundant. The diversity decreased after 30 days fermentation, and a total of 409 bacteria genera and 40 fungi genera were found in pit fermentation grains. The dominant bacteria at 30 days fermentation were Lactococcus (21.3%), Enterococcus (21.71%), Bacillus (10.92%) and Mycolicibacterium(8.26%), etc. The dominant fungi were Schizosaccharomyces (22.26%), Aspergillus (19.58%), Byssochlamys(14.64%) and Pichia (13.78%). The KEGG analysis showed that carbohydrate metabolism and amino acid metabolism were the main metabolic functions of microorganism during pit fermentation. Aspergillus, Moesziomyces, Pichiahadstrong positive correlations with the two major metabolic functions, while the Lactococcus, Bacillus and Mycolicibacterium were negatively correlated with them. Theseresults could provide an important reference for the analysis of dominant fermentation microorganisms and their metabolic functions in the formation of Maotai-flavor.
    19  High-density Cultivation of Lycopene-producing Engineered Bacteria in 5 L Fermenter and Optimization of Fermentation Conditions
    LI Yu LI Yi-meng YANG Li JIANG Jian-guo
    2020, 36(6):137-146. DOI: 10.13982/j.mfst.1673-9078.2020.6.0741
    [Abstract](405) [HTML](226) [PDF 1.10 M](1674)
    Abstract:
    In this study, the fermentation conditions of lycopene-producing engineered Bacteria W-05 were optimized. Firstly, single factor experiments were used to determine the type of culture medium, culture temperature, preferred sources of carbon and nitrogen, and inorganic salt. Then, according to the results of the single factor experiments, the response surface experiments were set up to determine the interaction between various factors. The results of the response surface experiments showed the components of the medium: peptone 10.00 g/L, yeast extract powder 5.00 g/L, glycerol 7.80 mL/L, ammonium nitrate 3.30 g/L, KH2PO4 1.80 g/L, and NaCl 11.62 g/L, under which, the lycopene yield reached a theoretical value of 3.42 mg/L. W-05 was then cultured at a high density using the optimized medium in a 5 L fermenter, and the experimental results showed the optimal fermentation conditions: pH 7.0, dissolved oxygen percentage about 20%, and the use of exponential fed-batch strategy. Under these conditions, the biomass and lycopene production of W-05 increased significantly (p<0.05), compared with those obtained via batch fermentation. The dry weight of bacterial cells reached 16.55 g/L and lycopene yield reached 19.93 mg/L after the optimized fermentation for 29 h. These results show that improving the medium composition and fermentation conditions can greatly increase the yield of lycopene produced by engineered bacterium W-05.
    20  Comparative Analysis of Strong-flavor Baijiu Pit-mud Microbial Community Using High-throughput Sequencing Based on 16S rDNA V4 and V3-V4 Variable Regions
    WANG Yan-li ZHANG Hui-min MENG Ya-jing ZHOU Qing-wu LIANG Jin-hui YUAN Zhi-qiang LIU Guo-ying HE Hong-kui LI An-jun
    2020, 36(6):147-154. DOI: 10.13982/j.mfst.1673-9078.2020.6.1130
    [Abstract](303) [HTML](376) [PDF 786.31 K](826)
    Abstract:
    In this study, Illumina high-throughput sequencing of 16S rDNA V4/V3-V4 variable regions of old and young pit-mud (PM) samples were performed. The diversity indices, NMDS clustering results, and the relative abundance of dominant phyla/genera of two variable regions were compared. The number of OTUs in V4 (264~639) were significantly lower than that of V3-V4 (459~1123). The Shannon indices of V4 in the young (2.47) and old (5.02) Wall-PM (WPM) samples were significantly lower than those of V3-V4 (3.33 and 6.46, respectively). Insignificant difference (p>0.05) was found between the young (4.98vs5.98) and old (5.64vs5.39) bottom-PM (BPM) samples. The Chao1 indices of V4 in the young BPM (2455vs1358), young WPM (1326vs504) and old BPM (3475vs1260) were significantly higher than those of V3-V4, with insignificant difference (p>0.05) in the old WPM samples (1915vs1312). The sequencing of the two variable regions had no effect on the NMDS clustering results. The heat-map clustering results showed that the relative abundance of the dominant phyla/genera for two variable regions were pretty similar, while it was easier for theV4 variable region to distinguish old BPM samples at the genus level. Compare with the V3-V4 region, the V4 variable region was more conducive to distinguishing between WPM and BPM and detecting the content of methanogen, which allows efficient analysis of the PM microbial composition. This study provided a good reference for the selection of V3-V4 and V4 regions during high-throughput sequencing of 16S rDNA.
    21  Isolation and Identification of Sulfur-oxidizing Bacterium from Brewery Wastewater and Its Sulfur-oxidizing Characteristics
    HAN Jia-min LUO Jian-fei LIN Wei-tie
    2020, 36(6):155-160. DOI: 10.13982/j.mfst.1673-9078.2020.6.0669
    [Abstract](177) [HTML](216) [PDF 800.87 K](631)
    Abstract:
    In this study, a chemoautotrophic sulfur-oxidizing bacterial strain LS2 was isolated from brewery wastewater. The analyses of 16S rRNA gene and sulfur-oxidizing enzyme gene soxB revealed that this strain was most closely related to genus Halothiobacillus, although the genome of Halothiobacillus sp. LS2 had a complete gene cluster encoding nitrogen-fixing enzyme Nif; Its key gene nifH had the highest similarity to A. ferrooxidans and A. ferrivorans in the genus Acidithiobacillus (both of which were higher than 80%). A comparative study at the cellular level indicated that the strain LS2 was able to grow under both nitrogen and nitrogen-free conditions. The maximum growth of LS2 under the condition of 1 mM NH4+ could reach 1.98×107/mL, whilst the growth in the absence of nitrogen (up to 1.37×107/mL) was less than that in the presence of nitrogen. The gene expression analysis revealed that nifH expression of strain LS2 was down-regulated due to the presence of a nitrogen source but up-regulated with the increase of thiosulfate concentration. The expression of nifH was up-regulated by 33 times at a S2O32-concentration of 5 mM, indicating that nitrogen fixation was closely related to sulfur oxidation. In this study, a chemoautotrophic sulfur-oxidizing bacterium with nitrogen-fixing ability was obtained, and the obtained results can provide a new idea for the treatment of sulfide-containing wastewater.
    22  Enzymatic Production and Lactobacillus Proliferation of Galactooligosaccharidesby β-Galactosidase from Lactobacillus plantarum in vitro
    ZHANG Hong-zhi MA Yan-hong ZHOU Jian-zhong LIU Xiao-li
    2020, 36(6):161-171. DOI: 10.13982/j.mfst.1673-9078.2020.6.1258
    [Abstract](389) [HTML](345) [PDF 1.33 M](848)
    Abstract:
    Enzymatic synthesis of prebiotic galactooligosaccharides (GOS) using the β-galactosidases produced from Lactobacillus plantarum was investigated. In this work, the effects of synthetic conditions including concentration of lactose substrate, reaction temperature, pH of system, enzyme concentration on the formation and the monitoring of the proliferation of lactic acid bacteria in vitro, total output and optimum conditions of different pure components of GOS were evaluated. Its probiotic activity was characterized. The results showed that the major reaction conditions were concentration of substrate, temperature, whereas pH and concentration of enzyme were secondary conditions. The optimal conditions for the total output was listed as followed: 45°C, substrate concentration of 400 g/L, pH 7.0, enzyme concentration of 10 U/mL, time of 10 h. Under these conditions, the total GOS output was 177.26 g/L, accounted for 44.31% (m/V) in the reaction system, including allo-lactose, 6′-galactobiose, 6′-GOS and 3′-GOS, they were 63.22 g/L, 15.32 g/L, 72.21 g/L and 25.11 g/L, respectively. For the proliferation of lactic acid bacteria in vitro, the new GOS was more remarkable by comparison to the commercialized GOS, as exhibited in the same amount of microbodies at relatively low concentration of GOS and the obvious advantage of maximum specific growth rate.
    23  Mechanism of Accelerating the Formation of Preserved Egg White Gel by Vacuum Decompression
    ZHANG Xiao-wei LIU Xu-hui GAO Jie LIU Hui-ping XU Xiang-xin TIAN Wen-tan
    2020, 36(6):172-180. DOI: 10.13982/j.mfst.1673-9078.2020.6.1151
    [Abstract](374) [HTML](330) [PDF 858.18 K](915)
    Abstract:
    The vacuum decompression method can significantly shorten the processing period of preserved eggs, but the mechanism of gelatin formation of egg white gel under this technology is unknown. Thus, in this paper, to explore the gel formation mechanism, water migration in egg white, protein conformational change, microstructure and intermolecular force changes were investigated, during the gelatinization process of egg white of preserved egg using the vacuum decompression method. Results showed that, during the pickling process of preserved egg, the hydrophobicity of egg white protein first increased and then decreased, the proper hydrophobic interaction was helpful to maintain the stability of the protein gel structure. The turbidity of egg white protein was significantly increased while the solubility was significantly reduced, the Zeta potential was increased by 1.13 times, the increase in electrostatic repulsion was beneficial to the formation of ordered linear protein aggregates, forming a transparent gel; the relative content of α-helical was reduced to 13.00%, while the β-sheet and β-turn structure were increased to 54.00% and 20.40%, respectively, the α-helix structure is transformed into β-structures to participate in the aggregation between protein molecules; the intermolecular forces that maintain protein gels in order from strong to weak were ionic bonds, disulfide bonds, hydrophobic interactions, and relatively few hydrogen bonding; through the above interactions, preserved egg whites finally formed a regular three-dimensional fiber network gel structure embedded with a large amount of bound water.
    24  Modeling and Analysis of the Effect of Steaming Time on the Quality of Yak Meat
    WANG Ke ZHU Chao-zhi ZHAO Gai-ming TIAN Wei ZHANG Wen-hua MA Jin-shou
    2020, 36(6):181-189. DOI: 10.13982/j.mfst.1673-9078.2020.6.1248
    [Abstract](299) [HTML](183) [PDF 808.22 K](549)
    Abstract:
    The yak meat with the same initial conditions was steamed for 40 min, 60 min, 80 min, 100 min and 120 min under the same temperature conditions. The tenderness, water-holding capacity (WHC), pH, color, protein content, fat content and moisture content were analyzed. The quality evaluation model of the steamed yak meat was constructed by principal component analysis combined with sensory evaluation. The sensory score was comprehensively compared with fuzzy mathematics. The results showed that the color, tenderness and WHC of yak meat were significantly affected by steaming time. Steaming for 60 min was more suitable for the traditional yak beef steaming process. The shear force of beef under this condition was 5.33 kg, with a color value of 12.15 and a WHC of 91.45%, which were all optimal. Its protein content, fat content and moisture content were 37.01%, 3.71% and 57.80%, respectively, which were all in reasonable range. The quality of the steamed beef with different steaming time was judged by PCA method: 60 min>80 min>40 min>100 min>120 min, which was significantly positively correlated with fuzzy mathematics sensory scores, further confirming the objective reliability of this model.
    25  Effect of Raw Material Properties on Cookie Quality and Acrylamide Generation
    ZHANG Jing ZHAO Yuan-yuan LI Xiao-ding RONG Jian-hua
    2020, 36(6):190-197. DOI: 10.13982/j.mfst.1673-9078.2020.6.1239
    [Abstract](249) [HTML](282) [PDF 760.69 K](794)
    Abstract:
    Flour, butter and sugar were used as main raw materials to make cookies. Moisture content, color, texture, sensory and acrylamide content were measured to investigate the effects of raw material characteristics on cookie quality and acrylamide generation. The results showed that the protein content in the flour significantly affected the quality and acrylamide generation of the cookies. Compared with high-gluten flour and medium-gluten flour, the low-gluten flour had a crude protein of only 7.67%. The cookie produced from the low-gluten flour had low moisture content, low hardness, better softness and crispness. The cookies were of the best quality with the lowest level of acrylamide. Cookies made from ordinary butter and fermented butter were crispy, with rich fragrant and good quality. A slower oxidation of fat and a low acrylamide content of 212 μg/kg were found in cookies made from fermented butter during baking. Cookies made from brown sugar were tan with a unique fragrance, but the acrylamide content reached as high as 828 μg/kg. Cookies made from white sugar, rock sugar and soft sugar demonstrated slight difference in quality. Among them, cookies made from white sugar had the highest sensory evaluation score, and the lowest acrylamide content of 230 μg/kg. In summary, cookies made from low-gluten flour, fermented butter, and caster sugar were of better quality and had less acrylamide production.
    26  Improvement of Emulsion Stability and Digestion Properties by Adjusting Wheat Protein Composition
    HUANG Yun-qi YANG Xiao-quan
    2020, 36(6):198-203. DOI: 10.13982/j.mfst.1673-9078.2020.6.0969
    [Abstract](327) [HTML](277) [PDF 1.10 M](778)
    Abstract:
    In this study, wheat protein-based food emulsion system with stable structure and delayed lipid digestion were constructed by controlling the gliadin-to-glutenin ratio. The results showed that among the wheat protein components, gliadin had good interfacial properties, and could form a stable interfacial layer. Although the interfacial activity of glutenin was poor, it could form a strong network at the interface and protect the emulsion from external environmental disturbances. Compared with the pure gluten-based emulsion, the composite protein with a controlled wheat protein composition had lower interfacial tension, forming a dense fibrous protein interfacial layer and smaller emulsion droplets. When the ratio of gliadin to glutenin was 1:2, the droplet size of emulsion samples remained 28.33 μm after 1 month of storage at 4 ℃or after being heated at 90 ℃ for 30 min, with the storage stability and thermal stability of emulsions being improved significantly. In addition, the complex interfacial layer formed by interfacial synergy between wheat protein components was difficult to be replaced by bile salts and pancreatic lipase during gastrointestinal digestion. Therefore, the stability of the emulsion could be improved and the digestion rate of the lipids in the emulsion could be inhibited to some extent by controlling the wheat protein composition.
    27  Effects of Olive Pomace on the Quality of Chinese Dried Noodles
    LUOSANG Zhuo-ma ZHANG Hua-ling HUANG Jun-tong CHI Yuan-long YAO Kai JIA Dong-ying
    2020, 36(6):204-210. DOI: 10.13982/j.mfst.1673-9078.2020.6.1163
    [Abstract](264) [HTML](280) [PDF 920.26 K](674)
    Abstract:
    In this paper, the contents of major chemicals in ground olive pomace were determined, and its effects on the cooking, textural, tensile and sensory properties of Chinese dried noodles were studied. The pomace contained 43.29% of crude fiber, 12.71% of crude fat, 4.54% of crude proteins, 0.72% of potassium, 1.67% of total flavonoids, 1.13% of total phenols and some other chemicals. Different amount of ground olive pomace can influence the quality of the noodles to varying degrees. Compared with the blank control, the best cooking time, water absorption, hardness, elasticity, stickiness, resilience, shear properties and sensory scores of noodles did not change significantly when the amount of olive pomace powder was 5%, but the cooking loss rate, chewability, cohesion and tensile properties decreased significantly; when the amount of olive pomace powder was 20%, the best cooking time of noodles is 1.25 min shorter than that of the blank control, the cooking loss increased by 17.60%, but the water absorption, hardness, elasticity, stickiness, cohesiveness, resilience and chewability decreased by 23.45%, 5.74%, 8.77%, 22.79%, 24.64%, 28.21% and 41.46% respectively; and the maximum breaking force, breaking distance, shear force and sensory score decreased by 26.51%, 38.65%, 22.57% and 31.87% respectively, compared to the control. Appropriate addition (≤10%) of ground olive pomace in noodles production can result in acceptable impact on their quality, but its excessive addition can significantly lower the quality of the noodles.
    28  Preparation and Antioxidant Activity of Bergamot Essential Oil Liposomes
    HUANG Wei-chao TANG Niang LIU Jing-yi ZHOU Ai-mei HUANG Kai-xin CHEN Shu-xi
    2020, 36(6):211-218. DOI: 10.13982/j.mfst.1673-9078.2020.6.1147
    [Abstract](480) [HTML](188) [PDF 818.01 K](716)
    Abstract:
    Using soybean lecithin and cholesterol as wall materials, bergamot essential oil liposomes were prepared by membrane dispersion and high pressure homogenization. The parameters of preparation and formulation were selected according to the particle size, zeta potential and entrapment efficiency of liposome, and the optimal preparation process was determined. The morphology and stability of liposome were investigated, and its antioxidant activity was also studied. The optimal process parameters were obtained as follows:Homogenization pressure 120 MPa, homogenization times 5 times, phospholipid/cholesterol ratio 5:1, essential oil addition amount 3.5 mg/mL. The particle size of the liposome prepared under these conditions was 183.53 nm, the PDI polydispersity index was 0.147, the zeta potential value was 38.75 mV, encapsulation efficiency was 47.23%, and drug loading was 1.67 mg/mL. The liposomes had good centrifugation stability and storage stability for 30 days at 4 ℃, the retention rate of essential oil was 85.03%, and the scavenging rates of DPPH, ABTS and hydroxyl radicals were 82.58%, 78.02% and 70.89%, respectively. Conclusion: Under the conditions of homogenization pressure of 120 MPa, homogenization times of 5, ratio of phospholipid to cholesterol of 5:1, and the amount of essential oil of 3.5 mg/mL, the liposome prepared has moderate particle size, good stability and good antioxidant activity.
    29  Gas Cooking Technology of Crucian Carp Nutrition Soup
    HAN Zhong DAI Lin-xue YU Xu-cong SUN Da-wen CHENG Jun-hu
    2020, 36(6):219-225. DOI: 10.13982/j.mfst.1673-9078.2020.6.0845
    [Abstract](292) [HTML](229) [PDF 710.32 K](578)
    Abstract:
    To study the effects of boiling power and boiling time on the nutritional and health benefits of traditional dishes and double-stewed soup, crucian carp was used as the raw material, and three low-power fire modes (power 300 W, 500 W & 700 W) were selected. The changes in contents of nutrients and flavor substances such as water-soluble proteins, fat, free amino acids and inosinic acid (IMP) were determined after different boiling times (0.5 h, 2.5 h and 4.5 h). The obtained results showed that the longer the boiling time, the more nutrients and flavor substances dissolved concomitant with an increased purine content. Compared with boiling power 700 W and 500 W, 300 W was beneficial for the dissolution of the water-soluble proteins, free amino acids and inosinic acid(IMP). In terms of the purine content, the content for 700 W was almost twice as much as for 500 W. According to the nutritional and health requirements of crucian carp soup, the optimal cooking conditions were: boiling power 500 W, boiling time 2.5 h. Under these conditions, the content of water-soluble protein was 0.49 mg/mL, the content of free amino acids was 0.32 mg/mL, the content of inosinic acid (IMP) was 0.16 mg/mL, the content of fat was 0.63 mg/100 mL, with the total purine being 10 μg/mL. Cooking in this way can ensure sufficient nutrients and desirable flavor in crucian carp soup, while meeting the required control of the purine intake by gout patients.
    30  Optimization and GC-MS Analysis of Extracting Pepper Leaves Essential Oil
    FAN Zhi-guo CHEN Chang-kai ZHAO Feng-xiang WU Ying-ying JIANG Zhi-guo SHI Rui-cheng
    2020, 36(6):226-234. DOI: 10.13982/j.mfst.1673-9078.2020.6.1260
    [Abstract](348) [HTML](348) [PDF 843.70 K](844)
    Abstract:
    Essential oils were extracted from pepper leaves by microwave-assisted distillation using different types of deep eutectic solvents as solvents. Different types of eutectic solvents were compared, and the main factors affecting the yield of essential oils (solvent-solid ratio, microwave power, extraction time) were optimized by single factor test and response surface methodology. The results showed that the choline chloride-urea deep eutectic solvent was the best extractant, when the conditions was as follow: solvent-solid ratio of 16:1 (mL/g), the microwave power of 480 W, and the extraction time of 53 min. Under these conditions, the maximum yield of essential oil was 1.42%. The comparison test indicated that the yield of the essential oil was significantly better than that of steam distillation and microwave-assisted distillation, which increased by 17.48% and 5.66%, respectively. The chemical constituents of the essential oils were identified by gas chromatography-mass spectrometry (GC-MS). A total of 34 substances, the main components were isospathulenol (25.47%), δ-elemene (17.52%), caryophyllene (8.27%), α-cubebene (7.07%), α- gurjunene (5.59%), (+)-ledene (5.02%). A method for extracting pepper leaves essential oil by using choline chloride-urea as deep eutectic solvent could be feasible.
    31  Optimization of Process Parameters for Chinese-style Beef Jerky Production
    ZHU Xiao-bing GUO Rui
    2020, 36(6):235-242. DOI: 10.13982/j.mfst.1673-9078.2020.6.1265
    [Abstract](456) [HTML](233) [PDF 729.31 K](1249)
    Abstract:
    In order to produce the Chinese-style beef jerky with the best edible quality, the process parameters of Chinese-style beef jerky were optimized. The fresh beef was used as the main raw material. The process of Chinese-style beef jerky was optimized by exogenous enzyme tenderization. The taste, appearance, shear strength, physical and chemical index, water ratio and water diffusion rate of beef jerky were used as evaluation indexes to analyze the effects of different production parameters on the quality of Chinese-style beef jerky. The results show that the optimal production process parameters of Chinese-style beef jerky were as follow: re-cooking time of 35 min, drying temperature of 49°C, the injection volume of bromelain tenderer with concentration of 4%, and the transglutaminase cross-linking time of 40 min. The Chinese-style beef jerky produced by the above optimized process parameters had suitable shearing value. The total sugar, protein and chloride content were the highest. The water, lead, inorganic arsenic, cadmium and other chemical substances were the least. The beef shrinkage rate and its temperature difference between the internal and external were small. The moisture diffusion rate of beef was high. Results indicated that the optimization of the production process by exogenous enzyme tenderization method and the selection of the optimal production process parameters could effectively improve the eating quality of Chinese-style beef jerky.
    32  Pearl Powder Improves the Quality of Yellow Rice Wine
    HAN Ji-wei ZHENG Da-heng LUO Wen PENG Qi SUN Jian-qiu
    2020, 36(6):243-248. DOI: 10.13982/j.mfst.1673-9078.2020.6.1081
    [Abstract](216) [HTML](348) [PDF 602.97 K](584)
    Abstract:
    In order to investigate the effect of pearl powder on the quality and antioxidant function of yellow rice wine, pearl powder was added into the rice wine fermented for 15 day of fermentation before further fermentation. The quality and antioxidant function of the fermented pearl-containing rice wine (termed “pearl rice wine”) were evaluated. The results showed that the contents of alcohol, total sugar, non-sugar solids, amino acid nitrogen and calcium ion in the pearl rice wine increased with the increase of the pearl powder content. There was an extremely significant difference between the wine with a high-dose of pearl and the blank rice wine (p<0.01). In terms of the total acid content, the high-dose group had the lowest (2.04 g/L); The medium-dose group had a relatively higher value 3.12 g/L, which was significantly lower than that of the blank group (p<0.01); The low-dose group had the highest 6.16 g/L, which was insignificantly different from the blank group (p>0.05). The high-dose pearl rice wine had good taste, color and significant antioxidant activity. It could significantly enhance the scavenging rates of superoxide anion radicals and hydroxyl radicals (57.54% and 66.18%, respectively), while significantly enhancing the activity of the SOD in serum and liver tissues of mice (p<0.05, p<0.01), and significantly reducing the content of MDA in serum and liver tissues (p<0.05, p<0.01). The research indicated that adding pearl powder (8 g/L) in the brewing process of yellow rice wine could significantly improve the quality and antioxidant function of yellow rice wine。
    33  Optimization of Fermented Watermelon-Tomato Juice by Compound Lactic Acid Bacteria
    LI Peng-cheng LIU Qing XIANG Yao-dong ZHANG Yao
    2020, 36(6):249-255. DOI: 10.13982/j.mfst.1673-9078.2020.6.1232
    [Abstract](354) [HTML](204) [PDF 713.40 K](711)
    Abstract:
    In this study, Lactobacillus plantarumS3-10 and Lactobacillus casei R10 isolated and screened from our lab were used as fermentation strains. The single factor and orthogonal experiments were used to study the major factors affecting the fermentation of watermelon-tomato, such as strain ratio, watermelon-tomato juice content, sucrose content, glucose content, temperature, etc. The results showed that the optimal technological parameters of combined fermentation watermelon-tomato juice by two strains of lactic acid bacteria were as follows: S3-10 and R10 strain ratio 1:2, watermelon-tomato juice 44.4%, sucrose 5%, glucose 6%, fermentation temperature 40 ℃. The number of viable cells obtained under this condition was 4.79×108 cfu/mL, and the total acid yield was 12.42 g/L, which was 21% higher than the initial total acid content. The organic acid compositions and changes analyzed by HPLC were similar in the fermentation process of watermelon-tomato juice by the two strains of lactic acid bacteria. The lactic acid and total acid content of the two strains of lactic acid bacteria compound fermentation was significantly higher than that of single strain fermentation. Considering the excellent performance of lactic acid bacteria S3-10 and R10 compound fermented watermelon-tomato, it has great potential and application prospects in the development of fruit and vegetable fermentation products and other functional probiotic products.
    34  Optimization of the Processing Technology of Orange Preserved Fruit
    Liu Ying
    2020, 36(6):256-263. DOI: 10.13982/j.mfst.1673-9078.2020.6.0148
    [Abstract](360) [HTML](294) [PDF 898.60 K](722)
    Abstract:
    Response surface method was used to optimize the processing technology of preserved orange fruit in this work. The sensory score and total sugar content were used as response values, and the effects of citric acid, calcium chloride and sugar concentration on preserved fruit technology were investigated. The box Behnken was used to optimize the influencing factors. The response surface analysis method of four factors and three levels were used, and the prediction model of quadratic polynomial regression equation was established. The influence of each variable interaction on the development process of tangerine preserves was studied to optimize the best development process of tangerine preserves. The optimum processing conditions of orange red preserved fruit were as follows: citric acid concentration of 0.30%, calcium chloride of 0.50%, microwave power of 210 W and sugar solution concentration of 50%. Under this condition, the sensory score and the total sugar content were 96.85, 46.13%, respectively, which were close to the theoretical value. It was proved that the parameters of the technology were accurate and reliable, which had a certain practical value.
    35  Comparative Analysis of Quality of Pleurotus eryngii Beef Dishes Treated by Different Parts of Beef
    PAN Zhi-li YU Ru-meng HUANG Zhong-min LI Hang-tian MA Jing-yi
    2020, 36(6):264-273. DOI: 10.13982/j.mfst.1673-9078.2020.6.1188
    [Abstract](279) [HTML](275) [PDF 865.20 K](726)
    Abstract:
    In order to study the effect of different parts of beef on the dishes quality of Pleurotus eryngii, this study selected the beef from the following five parts: sirloin, brisket, hind legs, cucumber strips and shoulder meat, and used the same technology to make Pleurotus eryngii beef dishes, to explore the effect of different parts of beef on the dishes quality. In this paper, texture analyzer, electronic nose, GC-MS and SEM were used to study the tenderness, texture, flavor and microstructure. In terms of tenderness, the tenderness of the hind legs of cattle was the highest, followed by cucumber strips. In the aspect of texture, the quality of loin was the best, followed by cucumber strip; in the aspect of flavor substance, the volatile flavor substance detected by analysis included 26 kinds of shoulder meat, 20 kinds of cucumber strip, 16 kinds of loin, 15 kinds of hind legs and 12 kinds of beef brisket; the electronic nose radar showed that there was no significant difference in the sensitive substance types of beef in five parts. In the aspect of microstructure, scanning electron microscopy showed that the muscle fiber gap and the degree of fracture were the largest in cucumber strips and shoulder. The sensory score of cucumber strip was 85. The results showed that there were 20 kinds of flavor substances, better tenderness and texture, and the highest sensory score in different parts of beef. Therefore, the quality of apricot abalone beef dishes made from the parts of cucumber was the best.
    36  Damage Characteristics and Bitter Substances Increase of Pomelo Juice Sacs Treating by Mechanical Extrusion
    CHEN Gao-hui LIN Xian XU Yu-juan YU Yuan-shan WU Ji-jun WEN Jing XIAO Geng-sheng
    2020, 36(6):274-284. DOI: 10.13982/j.mfst.1673-9078.2020.6.1049
    [Abstract](356) [HTML](228) [PDF 1.02 M](905)
    Abstract:
    In this study, the deformation degree of the pomelo juice sac was used as a parameter to investigate the effects of different degrees of mechanical extrusion on the physical damage on Shatian pomelo, white flesh pomelo and red flesh pomelo, as well as their nutritional composition, color, and bitter substances including naringin and limonin. The results showed that mechanical extrusion had little effect on the color and total acid content of the three types of pomelo juice sacs. With the increase of the degree of mechanical compression, the juice loss rate, soluble protein content, naringin content, total phenol content, and total soluble solid content of the three types of pomelo samples increased, whilst their ascorbic acid content and protopectin content decreased. The soluble pectin content and fat content reached their maximum when the degree of deformation caused by mechanical damage was 40%. Compared with the white flesh pomelo and red flesh pomelo, mechanical damage decreased the total sugar content by 2.89%, increased limonin content by 64.12% in Shatian juice sacs, with a greater increment of naringin content (by 17.48%). This study indicated that the greater compression the mechanical extrusion, the greater the damage to the pomelo juice sacs. The accumulation of bitter substances varied depending on pomelo varieties, with the increase of the bitter taste in Shatian pomelo juice sacs being greater.
    37  Identification of Three Listeria Species in Foods based on High Resolution Melting Curve Analysis
    YUE Yuan ZHOU Meng-shi XU Juan LI Rui HE Li-hua ZHAO Fei ZHANG Jian-zhong GONG Jie
    2020, 36(6):285-290. DOI: 10.13982/j.mfst.1673-9078.2020.6.1176
    [Abstract](433) [HTML](185) [PDF 701.50 K](566)
    Abstract:
    In this study, a new pair of primers was designed to identify Listeria monocytogenes, Listeria evanescens and Listeria innominata at the same time with iap gene as the target by using high-resolution fusion curve method. The amplification of the other 14 common foodborne pathogens was negative. The detection limit of Listeria monocytogenes and Listeria evanescens was 10 copies, and that of Listeria innominata was 50 copies. In this study, 78 samples were tested by HRM-real time PCR method, GB4789.30-2016 and SN/T 1870-2016 (real-time PCR), and the results of three methods were analyzed statistically. The results showed that there were statistical differences between the detection results of HRM method and national standard method, HRM method and fluorescent PCR method, and there was no statistical difference between the results of national standard method and fluorescent PCR method. The method based on HRM-real time PCR for the detection of three Listeria species in this study is fast, efficient, specific, and low cost. It is suitable for the daily detection and supervision of Listeria species in food.
    38  Simultaneous Detection of 7 Benzimidazoles Residues in Carp by Colloidal Gold Immunochromatographic Strip
    ZHANG Cai-qin ZHANG Xun LI Jie LI Na XIAO Xiang OUYANG Qin WANG Yun ZHOU Xing-hua
    2020, 36(6):291-296. DOI: 10.13982/j.mfst.1673-9078.2020.6.1263
    [Abstract](220) [HTML](292) [PDF 687.54 K](615)
    Abstract:
    In order to reduce its harm to human, a colloidal gold immunochromatographic strip was established based on monoclonal antibody (mAb) to detect the residue of benzimidazoles in carp. Firstly, the immunogen was prepared using 2-(methoxycarbonylamino)- 3H-benzimidazole-5-carboxylic acid as hapten and coupling protein by activated ester method, mice were immunized and mAb was prepared by cell fusion. Then A colloidal gold immunochromatographic strip was prepared by mAb labelled with synthetic colloidal gold. The 50% inhibitory concentrations (IC50) of albendazole, mebendazole, albendazole s-oxide, albendazole sulfone, fenbendazole flubendazole, oxfendazole were 0.44, 0.16, 3.47, 5.62, 0.62, 0.10, 5.77 ng/mL by ic-ELISA, respectively. The limit of detection (LOD) of albendazole, mebendazole, albendazole s-oxide, albendazole sulfone, fenbendazole, flubendazole, oxfendazole were 4, 2, 50, 100, 10, 1, 100 ng/g in carp samples, respectively. Therefore, the developed method was highly sensitive, rapid, low-cost, free-instrument and applicable for on-site detection of a large numbers of carp samples.
    39  Establishment of RT-LAMP-HNB Method for Listeria monocytogenes Detection
    XU Cong LUO Hua-jian HUANG Hao LIANG Wei-qu HU Shan LI Yan-fang HU Chu-wei LUO Hong-bin
    2020, 36(6):297-302. DOI: 10.13982/j.mfst.1673-9078.2020.6.1182
    [Abstract](327) [HTML](224) [PDF 689.19 K](627)
    Abstract:
    A rapid detection method RT-LAMP-HNB for Listeria monocytogenes was developed. Two pairs of primers were designed according to the conserved region of the hlyA gene sequence. In the same reaction system, the RNA of Listeria monocytogenes was reverse transcripted to cDNA and then was amplified by loop mediated isothermal amplification. At the same time, hydroxyl naphthol blue (HNB, the final concentration of 200 μM) was used as an indicator for reverse transcription loop mediated isothermal amplification products (RT-LAMP). The amplified results can be interpreted according to the color change of the system, and the gel electrophoresis was not necessary. This detection can be done within 20 hours (including the time of enrichment) and the live Listeria monocytogenes in milk can be detected. The detection limit in this study for the RNA of Listeria monocytogenes is 5.8×10-3 μg /mL, the detection limit of the initial inoculation concentration is 10 CFU/10mL, which are 10 times more sensitive than the RT-PCR method. RT-LAMP-HNB detection method developed in this study has the characteristics of fast, accurate, convenient and high sensitivity, which is suitable for popularization and application in grass-root labs or inconvenient areas.
    40  Origin Tracing of Red Fuji Apple Based on Near Infrared Transmission Spectrum and Various Dimension Reduction Methods
    MA Yong-jie GUO Jun-xian GUO Zhi-ming HUANG Hua SHI Yong ZHOU Jun
    2020, 36(6):303-309. DOI: 10.13982/j.mfst.1673-9078.2020.6.1249
    [Abstract](534) [HTML](237) [PDF 892.77 K](861)
    Abstract:
    In order to find a new method of tracing apple origin, in this work, 600 apple samples from three different producing areas of Aksu, Luochuan, and Yantai were used to collect the near-infrared transmission spectra within 590~1250 nm, then spectroscopically corrected spectrum were treated by eight species of spectral pretreatment such as normalization, standard normal variate transformation, multivariate scattering correction, savitzky-golay smoothing, 2nd derivative, mean centering, moving average, 1st derivative. Firstly, established full spectra classification model for pre-processed spectral data showed that 2nd derivative was the best pre-processing method. Secondly, data set preprocessed by 2nd derivative were used to combine 4 different KNN models (Euclidean, correlation, cosine, cityblock) to do pattern recognition, which was found that K-nearest neighbor method (correlation) was the best classification and recognition method. Thirdly, factor analysis, gaussian process latent variable model, linear local tangent space alignment, neighborhood components analysis, neighborhood preserving embedding, diffusion maps, t-distributed stochastic neighbor embedding, landmark isomap, laplacian eigenmaps, locally linear embedding, principal component analysis, linear discriminant analysis were used to reduce the dimension of the spectrum after 2nd derivative pretreatment, and then combining K-nearest neighbor was combined to trace the origin of apple. Results showed that an optimal identification model was obtained by using 2nd derivative-diffusion maps-KNN (correlation). The identification rates for the calibration set and prediction set were 97.3% and 92.3%, respectively. Therefore, the deep learning dimension reduction methods combined with near-infrared transmission spectroscopy could successfully and effectively discriminate the traceability of apple origin.
    41  Component Analysis of Vegetable Blended Oil Based on Standard Fingerprint and Characteristic Fatty Acids
    GAO Hai-dong GAO Huo-liang YIN Hong-na GAO Rui ZHANG Ya-xun LIU Bing-ge DONG Jian-min
    2020, 36(6):310-320. DOI: 10.13982/j.mfst.1673-9078.2020.6.1223
    [Abstract](346) [HTML](163) [PDF 840.83 K](781)
    Abstract:
    Taking soybean oil, corn oil, rapeseed oil and olive oil as research objects, the ternary compound model of soybean oil, corn oil, rapeseed oil and soybean oil, corn oil, olive oil was established. The standard fatty acid fingerprint was established by gas chromatography (GC) and common mode method, and the similarity analysis was carried out at the same time. The change of fatty acid content in different proportion of mixed oil was analyzed by gas chromatography and chemometrics. The results showed that the four standard fatty acid fingerprints established in this experiment could better reflect the commonness and difference of different oils. Palmitic acid (5.88%~10.72%), stearic acid (2.31%~4.57%), oleic acid (15.04%~77.45%), linoleic acid (5.92%~53.67%) and α-linolenic acid (0.61%~6.71%) were selected as the characteristic fatty acids. According to the correlation (R2>0.95) and significance (p<0.01), oleic acid and linoleic acid, α-linolenic acid and oleic acid were used as the representative fatty acids of the two ternary compound models. Through the example verification of the regression prediction model of representing fatty acids, the RSD% of the two ternary compound models was verified to be less than 8% and 10% respectively, which indicated that the regression prediction model has strong guiding significance and practical value. This study reflects the differences of fatty acids in different components of vegetable blended oil, which can provide theoretical basis for quality control and detection of edible oil.
    42  Determination of Vitamin K2 (MK-7) in Nutrient Supplements by High Performance Liquid Chromatography-Fluorescence Spectrometry
    SUN Xiao-fei ZHANG Xiu-mei GAO Yu-jing QIU Jie LIU Xin LI Shuai ZHANG Kun
    2020, 36(6):321-327. DOI: 10.13982/j.mfst.1673-9078.2020.6.1209
    [Abstract](590) [HTML](265) [PDF 708.27 K](682)
    Abstract:
    To establish a method for the determination of Vitamin K2 (MK-7) in Nutrient Supplements by high performance liquid chromatography-fluorescence detector (HPLC-FLD), the sample was extracted by isopropanol ultrasonic for 30 min, after centrifugation and filtration, 10 μL of the sample solution was injected into the liquid chromatography system. The sample solution was separated by Agilent C18 column (4.6 mm×150 mm, 5 μm) or equivalent column. After the column, it was derived by zinc powder reduction column (4.6 mm×50 mm), so that the Vitamin K2 (MK-7) in the sample had fluorescence characteristics. Methanol (containing 5% tetrahydrofuran, glacial acetic acid 0.03%, zinc acetate 1.0 g/L) was used as mobile phase, the flow rate was 1.0 mL/min, the fluorescence detector was set at λex/λem=243 nm/430 nm. When the sample weight was 1.0 g and the volume was 10 mL, the detection limit of this method was 0.03 μg/g, and the quantitative limit was 0.1 μg/g, MK-7 had a good linearity in the range of 0~2.176 μg/mL, the R2 was 0.9999. The relative standard deviation of the repeatability experiment was 0.97%, and the recovery of the standard was 93.40%. This method has simple operation, good accuracy, and good reproducibility. It can be used for the determination of MK-7 in Nutrient supplements.
    43  Research Progress in Preservation of Rambutan Fruit
    CHEN Jiao LI Fen-fang LI Yi-xing YUAN De-bao JIANG Yue-ming
    2020, 36(6):328-334. DOI: 10.13982/j.mfst.1673-9078.2020.6.1046
    [Abstract](384) [HTML](555) [PDF 607.20 K](758)
    Abstract:
    Rambutan fruit has good flavor and high nutritional value, and is promoted as one of the "four major fruits" by FAO. The fruit is extremely prone to water loss, browning, and rot during transportation and storage, which greatly affects fruit’s appearance and nutritional quality. The existing preservation technologies have problems such as single technology, weak pertinence, high application difficulty and insecurity. In this paper, the problems associated with rambutan fruit such as mechanical damage, water and weight loss, chilling injury, enzymatic browning, post-harvest diseases and insect pests are discussed in detail, and the pre-harvest, harvest and post-harvest preservation technologies are summarized, with the focus on commercialized treatment, and physical, chemical, biological and comprehensive preservation techniques. The recent research progress in preservation technologies for rambutan fruit domestically and abroad is overviewed. The development trend of research and application of the preservation technologies for rambutan in the future is prospected, which provides certain theoretical guidance for further research of the storage and preservation technologies for rambutan, especially the domestic rambutan.

    Editor in chief:李琳

    Inauguration:现代食品科技

    International standard number:ISSN 1673-9078

    Unified domestic issue:CN 44-1620/TS

    Domestic postal code:46-349

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