Volume 34,Issue 12,2018 Table of Contents

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  • 1  Inhibition of Cardiomyocyte Apoptosis in Aging Rats by Saponins of Panax japonicus via Activating Autophagy
    WANG Luo-pei ZHENG Jie LI Si-min ZHANG Chang-cheng WANG Ting YUAN Ding ZHOU Zhi-yong
    2018, 34(12):1-6. DOI: 10.13982/j.mfst.1673-9078.2018.12.001
    [Abstract](472) [HTML](255) [PDF 461.65 K](801)
    Abstract:
    The protective effects of total saponins extracted from Panax japonicus (TSPJ) on cardiomyocyte apoptosis in natural aging rats, as well as its underlying mechanism in terms of autophagy was investigated. Male SD rats aged 18 months were randomly divided into 3 groups (model group, low-dose TSPJ group and high-dose TSPJ group), 10 rats in each group. TSPJ groups were given TSPJ at different doses (10 and 30 mg/kg, p.o.), model group was treated with equal normal animal food for 6 months until 24 months old. Another 10 rats aged 6 month (6M) were used controlled group. Compared to young control group, the myocardial cells arranged in disorder, and the muscle fibers broke, and cardiomyocyte apoptosis increased by 3 times in the natural aging group. TSPJ significantly improved the morphology of heart tissue and decreased cardiomyocyte apoptosis by 55% in comparison with natural aging group. Protein expression levels of Bax, Caspase-3, Caspase-9 and p62were increased by 9.5, 1.8, 4.1 and 3.4 times, the protein expression levels of Bcl-2, Bcl-2/Bax, LC3Ⅱ/ LC3Ⅰand Beclin1 was deceaesed by 98%, 99%, 70% and 48% in the aging group. TSPJ can effectively inhibit the protein expression of Bax, Caspase-3, Caspase-9 and p62 by 87%, 65%, 70% and 65%, and increase the expression of Bcl-2, Bcl-2 /Bax, LC3Ⅱ/ LC3Ⅰand Beclin1 by 12.75, 138.5, 5.68 and 2.6 times in the natural aging heart tissues. The results suggest that TSPJ can effectively improve cardiomyocyte apoptosis in natural aging rats, and its mechanism may be related to regulation of autophagy.
    2  L-arabinose Promotes the Growth and Development of Caenorhabditis elegans and Reduces Fat Synthesis
    WU Chao WANG Hui ZHANG Xiao-wei QIN Hai-lan ZHOU Xing-hua ZHAO Yan-sheng
    2018, 34(12):7-11. DOI: 10.13982/j.mfst.1673-9078.2018.12.002
    [Abstract](634) [HTML](357) [PDF 638.11 K](696)
    Abstract:
    In this work, the effect of L-arabinose on the growth development and fat synthesis capacity of Caenorhabditis elegans was investigated. Glucose was used as control sample while 2.5, 5.0, 10.0 and 20.0 mmol/L of L-arabinose were added respectively as treatment groups. After pretreatment of synchronized, the head swing frequency, body bending frequency, body length, number of offspring, triglyceride content and oil red O staining of elegans were analyzed. The results showed that L-arabinose had no significant effect on the body bending frequency and number of offspring of Caenorhabdits elegans. However, it had a significant efficiency on the head swinging frequency and body growth of elegans. When the concentration was 20 mmol/L, the head swining frequency increased by 20%. When the concentration of L-arabinose was 7.5 mmol/L, the body length of elegans increased by 11%. The triglyceride analysis revealed that the contents of triglyceride in treatment groups were lower than that of control group and decreased gradually with increasing L-arabinose concentrations. When the concentration was 20 mmol/L, the triglyceride content in elegans reduced by nearly 67% compared with the control group. The results of oil red O staining were consistent with the analysis of triglyceride. Results indicated that L-arabinose can promote the head swining frequency and body growth of Caenorhabdits elegans and has obvious effect on reducing fat synthesis of them, thus has a certain lipid-lowering function.
    3  Protection of LPS-induced Intercellular Hyperpermeability in Caco-2 Cells by Polypeptide from Moringa oleifera
    SONG Jia-le QIAN Bo WANG Cheng-qiang ZEN Zheng LEI Shao-ling HUANG Ao
    2018, 34(12):12-18. DOI: 10.13982/j.mfst.1673-9078.2018.12.003
    [Abstract](553) [HTML](223) [PDF 673.28 K](596)
    Abstract:
    The protective effect of polypeptide from Moringa oleifera (MOPP) on lipopolysaccharide (LPS, 2 μg/mL) induced hypepermeability in Caco-2 cells was investigated. The cell survival rate was measured by MTT assay. The cellular level of lactate dehydrogenase (LDH) was determined by kit. Enzyme linked immunosorbent assay (ELISA) was used to determine the level of Interleukin (IL)-1β, IL-8, and tumor necrosis factor (TNF)-α. The transepithelialelectricalresistance (TEER) and fluorescein dextran (FD40) permeability were used to evaluate the permeability of cells. Quantitative real-time PCR (qRT-PCR) was used to detect the expression of IL-1β, IL-8, TNF-α, Occludin, claudin-1, ZO-1, and myosin light chain kinase (MLCK). A high concentration of MOPP (150 μg/mL) treatment significantly increased the cell survival rate (to 83.8%) and inhibited the spillover of LDH (50.3%) compared with that in model cells. MOPP treatment effectively inhibited the secretion of inflammatory cytokines (IL-1β: 19.8%, IL-8: 43.7% and TNF-α: 37.9%) and reduced their mRNA transcription (IL-1β: 44.3%,IL-8: 35.0% and TNF-α: 33.5%)in model cells. In addition, MOPP also significantly increased the mRNA levels of Occludin, claudin-1, ZO-1, and inhibit the 31.3% of MLCK mRNA transcription to improve the intercellular permeability in LPS treated Caco-2 cells. These results suggest that the MOPP exhibited a strong anti-inflammatory activity, and can improve the LPS induced high permeability of Caco-2 cells may associate with regulating the mRNA transcription of tight junction related factors.
    4  Preparation of Hydrophobic Modified Quinoa Starch Granules and Study on Emulsifying Properties of Pickering Emulsion
    XIAO Zhi-gang SHI Chao YANG Liu QIN Xin-sheng YANG Qing-yu LUO Zhi-gang
    2018, 34(12):19-25. DOI: 10.13982/j.mfst.1673-9078.2018.12.004
    [Abstract](678) [HTML](270) [PDF 3.68 M](1030)
    Abstract:
    In this study, quinoa starch was extracted from quinoa seeds by alkali method, and the extracted starch was modified by octenyl succnic anhydride (OSA), then the starch octenylsuccinate (OSA starch) was obtained. The structure and morphology of the native starch and OSA starch granules were compared by Fourier transform infrared spectroscope and scanning electron microscope. It was found that the OSA group was successfully attached to the surface of the starch, and the Particle surface was slightly damaged. And the effects of starch substitution degree, particle concentration and oil fraction on emulsion emulsification were analyzed by emulsion microstructure, droplet size and emulsifying index (EI). The results showed that the droplet size decreases with the increase of OSA starch substitution or starch granule concentration, and the EI value increases with the increase of OSA starch substitution degree or starch granule concentration, and the emulsion emulsifying properties is enhanced. As the oil fraction raise, the droplet size appears to increase, and within the range of the amount of OSA added by the food additive, the EI value of the OSA starch granule with substitution degree of 1.43% reaches a maximum of 75.48%, emulsifying properties were the best. These findings demonstrated that OSA modified quinoa starch can be used as a particle to stable Pickering emulsions and have great potential applications in food.
    5  Effect of Konjac Gum on Rheological Properties of Sweet Potato Starch and Qualities of Starch Noodles
    LONG Ming-xiu WU Feng-yu TIAN Zhu-xi LIU Min HE Yang-bo LI Yong-fu SHI Bin LIANG Qian
    2018, 34(12):26-33. DOI: 10.13982/j.mfst.1673-9078.2018.12.005
    [Abstract](633) [HTML](273) [PDF 1.06 M](834)
    Abstract:
    To provide theoretical guidance for improving the qualities of starch noodles, the effect of konjac gum (KGM) on the pasting, retrogradation, rheologicalpropertiesofsweet potato starch (SPS)and the qualities of sweet potato starch noodles were investigated. The viscosity of the mixture of SPS and KGM was assayed by rapid viscosity analysis (RVA) so as to analyse the pasting properties of the mixture system.The crystallinity was measured by X-ray diffractometer to evaluate the long-term retrogradation of the system. The effect of KGM on the viscoelasticity of the system was investigated by rheometer. The effect of KGM on the qualities of starch noodles was evaluated.The results showed thatKGM reduced the pasting temperature, increased the viscosity, breakdown and setback, and inhibited the long-term retrogradation of starch significantly. The complex SPS/KGM was yield-pseudoplasticity system with better viscoelasticity, with the increasing of the proportion of KGM, the yield stress and consistency coefficient increased, while the flow behavior index decreased. Overall, a mixture of sweet potato starch and konjac gum (8.5:1.5 g/g) proved to be the most optimal proportion.
    6  Construction and Characteristics of the Liquid Fermentation System Co-cultured by Eurotium cristatum and Alkaloids in Tea
    HUANG Hao ZHENG Hong-fa ZHAO Xi ZHONG Ni YU Peng-hui HUANG Jian-an LIU Zhong-hua
    2018, 34(12):34-39. DOI: 10.13982/j.mfst.1673-9078.2018.12.006
    [Abstract](557) [HTML](229) [PDF 975.52 K](849)
    Abstract:
    Using three representative compounds in tea (caffeine, theobromine and theophylline) as substates to construct a liquid fermentation system of Eurotium cristatum (E.C), the fermentation characteristics of E. C. were preliminarily investigated. Results indicated that the addition of caffeine (0.2 mg/mL) could stimulate the growth of E.C to a certain extent. The content of glucose in the fermentation liquor decreased rapidly, and the pH value progressively declined with the increase of fermentation time until the ninth day, while the content of soluble protein increased first and then decreased. During the fermentation, E.C could not consume the soluble protein as carbon or nitrogen source to maintain its growth, and metabolize caffeine. Similar to caffeine, the theobromine and theophylline (0.08 mg/mL) could also stimulate the growth of Eurotium cristatum to some extent, but could not be consumed as a carbon source. However, the caffeine with concentrations of 0.00091 and 0.00109 mg/mL was detected respectively in the fermentation broth of theobromine and the theophylline, implying that Eurotium cristatum had the ability to synthesize caffeine with precursor of theobromine and theophylline.
    7  Effect of Heat-moisture Treatment on the Multi-scale Structure and Digestibility of Rice Starch with Different Amylose Content
    BIAN Hua-wei LIU Yu-fan ZHENG Bo ZHU Hui-lian CHEN Ling
    2018, 34(12):40-44. DOI: 10.13982/j.mfst.1673-9078.2018.12.007
    [Abstract](638) [HTML](269) [PDF 546.01 K](978)
    Abstract:
    In this paper, the relationship between multi-scale structure and digestibility of rice starch with different amylose content before and after the heat-moisture treatment (HMT) was investigated. The results showed that the thermal energy and water molecules during HMT could degrade starch molecules, unravel double helix, reduce relative crystallinity and decrease the ordered degree of lamellar structure, and the Mw>2×107 area gradually turned to the Mw<1×106. And these changes were more obvious with the lower amylose content of rice starch. Accompanying the structural disorder and degradation, some molecule rearrangements and orientation occurred to probably form densely packed starch fractions such as new single helix structure, which was conducive to improve the content of slowly digestible starch (SDS) and resistant starch (RS), and these changes were more obvious with the more amylose content of rice starch. The results provide basic data and theoretical support for the processing of starch and starch-based nutritional healthy food.
    8  Comparative Analysis on the Suitability of Fresh-cut Vegetable Processing for South China Ecotypic Cucumber
    GAO Jia ZHU Yong-qing LUO Fang-yao DENG Qian-jiang LIANG Gen-yun LIU Xiao-jun
    2018, 34(12):45-52. DOI: 10.13982/j.mfst.1673-9078.2018.12.008
    [Abstract](542) [HTML](318) [PDF 375.38 K](576)
    Abstract:
    In order to evaluate the suitability for fresh-cut vegetable processing of cucumber, 18 South China ecotypic cucumber valrieties and a control cultivar (Yanbai cucumber) were planted and harvested. Twelve indices of the fruits were measured, which included moisture content, total soluble solids content (TSSC), flesh thickness, seed cavity diameter, fresh color (L*, a* and b* value), peel and flesh firmness, sensory index (taste feeling and brittleness) and production rate. The main evaluation factors of suitability selection for fresh-cut cucumber processing were determined by statistical analysis results, correlation analysis and factor evaluation, and their weight was determined by analytic hierarchy process. The suitability of 19 cucumber samples for fresh-cut processing was sorted by grey relational analysis. Combined with the field cultivation characteristics of each sample, the suitable fresh-cut samples were selected as 4# and 2#. The above results can provide scientific basis for the selection of varieties of fresh-cut cucumber processing, and also provide guidance to the selection of breeding of special varieties for fresh-cut South China ecotypic cumber.
    9  Characterization of Nanofiller on Multi-scale Structures and Thermal Property of Starch Ester Film
    ZHANG Shu-yan LIU Yu-jia ZHU Jie LI Lin
    2018, 34(12):53-57. DOI: 10.13982/j.mfst.1673-9078.2018.12.009
    [Abstract](582) [HTML](305) [PDF 2.79 M](629)
    Abstract:
    The starch-based film was prepared by solvent-casting method. G50 starch ester with high degree of substitution was plasticized by triacetin and composited with organically modified montmorillonite (Dellite 72T). The features of fractured surface of the nanocomposites and the dispersion of nanofiller were analyzed by SEM and TEM. FTIR, SAXS and XRD techniques were used to characterize the molecular structures, ordered aggregation region and crystalline structures. The thermal property of starch ester film was detected by TG technology. The results indicated that some wrinkles of macromolecular aggregation were developed after the nanofiller addition, which was caused by the increasing interaction between nanofillerand G50 starch ester. The layered structures with different thickness (d≈12.57 nm, 19.68 nm) were observed in the nanocomposite,while the crystalline structures of film and the layered structures of nanofiller were scarcely changed, and simultaneously, partly intercalated structures were formed. Therefore, the thermal stability of nanocomposite was improved due to these featured aggregation structures. These findings could be helpful to the further application of starch-based materials in food packaging industry via the structural modification of starch.
    10  Purification and Thermal Stability of Anthocyanin from Hibiscus sabdariffa L.
    YU Yi-gang LIANG Ze-ming WAN Zhi-chao LIANG Yu-si YU Xiang-xiong WANG Cheng ZHANG Qing
    2018, 34(12):58-66. DOI: 10.13982/j.mfst.1673-9078.2018.12.010
    [Abstract](528) [HTML](189) [PDF 750.41 K](913)
    Abstract:
    In this study, the conditions for the purification of anthocyanins from Hibiscus sabdariffa L. and the thermal stability of these anthocyanins in the presence of different stabilizers were investigated using roselle anthocyanins as raw materials. The results showed that the optimized conditions for the purification of anthocyanins were: Loading concentration, 600 mg/L; Equilibrium time, 3 h; Loading volume,183 mL; eluent, 60% aqueous ethanol; elution flow rate, 1 mL/min. The color value of the lyophilized powder of purified anthocyanins was 43.10±2.17, with a recovery of (83.62±5.72)% and anthocyanin content of 216.50±1.83 mg/g. The degradation of three systems containing roselle anthocyanins together with 1.0% sodium alginate, carboxymethylcellulose (CMC) or β-cyclodextrin at 80, 90 or 100 ℃ all followed the first-order kinetics. The degradation rate constant increased with elevated temperature while the half-life decreased with increasing temperature. β-Cyclodextrin had the potential to delay the degradation of anthocyanins. The degradation rate of anthocyanins became smaller and smaller with an increase of β-cyclodextrin concentration. After heating at 80 ℃ for 150 min, the a* value of the anthocyanin solutions decreased while the b* value increased. An increase of β-cyclodextrin concentration facilitated the maintenance of the redness of anthocyanins, and 1.5% β-cyclodextrin exhibited the most effective protection.
    11  Effect of Locust Bean Gum on Water Distribution and Bread Quality of Frozen Dough
    GONG Wei WU Lei-yan ZHONG Ya-yun ZHOU Jin-feng TU Jin DONG Wu-hui LUO Deng
    2018, 34(12):67-73. DOI: 10.13982/j.mfst.1673-9078.2018.12.011
    [Abstract](555) [HTML](251) [PDF 4.94 M](978)
    Abstract:
    In order to study the effect of water-holding locust bean gum on the water distribution and discusse the relationship between micro-network structure, texture characteristics and water distribution of dough, the locust bean gum (LBG) was added to the frozen dough, the moisture distribution and state of the frozen dough was measured by NMR, the microscopic network structure of dough was determine by scanning electron microscopy, and the structure characteristics of the bread were analyzed by the scanner and texture analyzer. The results showed that with the increase of frozen weeks, the proportion of deep combined water in the dough decreased from 17.40% to 14.40%, while free water increased by 3.40%. The ability of gluten to encapsulate starch granules gradually decreased, the arrangement of starch granules was gradually confusing, the fermentation speed of the dough was gradually slow, and the texture structure of the bread was no longer uniform, the hardness was increased, and the elasticity was decreased. After adding locust bean gum, the proportion of deep combined water in the dough decreased from 17.20% to 15.40%, while free water increased by 2.70%, the deterioration of the frozen dough and its baked bread was reduced, indicating that the storage characteristics of the frozen dough was changed and the conversion of deep combined water in the dough was effectively delayed by LBG.
    12  Effects of Waterless Keep Alive Time on Physiological and Biochemical Index of Ictalurus punctatus
    LI Ning BAI Chan XIONG Guang-quan ZHANG Jin-mu WANG Ju-guang ZHANG Cheng GENG Sheng-rong WANG Cai-xia LIAO Tao
    2018, 34(12):74-81. DOI: 10.13982/j.mfst.1673-9078.2018.12.012
    [Abstract](439) [HTML](211) [PDF 1.41 M](666)
    Abstract:
    The effect of waterless keep alive time on biochemical index and muscle quality of Ictalurus punctatus was investigated. Optimum keep alive conditions of carbon dioxide and survival rate at different keep-alive time without water were studied in this experiment. The quality indexes of blood biochemistry and fish protein, fat and water content under different keep alive time (5, 7, 9, 11 h) were analyzed before and after waterless keep alive and 24 h after recovery. The results showed that the optimum concentration of CO2 and temperature of waterless keep alive are 500~550 mg/L and 4~8 ℃. The anesthetized Ictalurus punctatus with carbon dioxide were kept alive at 6 ℃ for 5, 7, 9, 11 and 13 h respectively and then transferred to clear water to recover for 24 h. The survival rate is 100%, 100%, 95%, 45% and 20% respectively. As the keep alive time increases, water loss and water holding time have no significant changes (p<0.05). The pH, protein and crude fat in muscle were lower than those in control group, but the content of muscle glycogen was significantly decreased (p<0.05). The activities of urea nitrogen (BUN), creatinine (Cr), pyruvate kinase (PK), lactate dehydrogenase (LDH) and transaminase (GOT) in waterless keep alive group were significantly higher than those in the control and recovery groups(p<0. 05). The activity of GOT and BUN is most closely related to the keep alive time. When the keep alive time was 9 h, GOT increased from 18.48μmol/L to 25.09 μmol/L, BUN increased from 13.18 mmol/L to 16.44 mmol/L, and their activities increased significantly (p<0.05). After recovery in clear water for 24 h, the blood indexes of Cr, BUN and GOT basically recovered to the levels of the control group(p>0.05). When the keep alive time was 11h, their activities of Cr, BUN and GOT increased significantly (p<0.05), which was 52.41%, 25.48% and 48.24% higher than those of the control group respectively. Conclusion: The best keep alive time anesthetized with carbon dioxide is 9 h. The metabolism of Ictalurus punctatus is slow and the toxin can’t be discharged in vitro with the prolongation of the waterless keep alive time, and it will cause irreversible damage to the kidney and liver. The results in this study would provide information for studying the application of waterless keep alive transportation of Ictalurus punctatus.
    13  Effects of Wheat Bran Particle Size on the Rheological Property of Whole Wheat Dough and Buns Quality
    LIU Li-ya YUE Ying LIN Yan-jun ZHOU Xian-rong TONG Li-tao WANG Li-li ZHOU Su-mei
    2018, 34(12):82-88. DOI: 10.13982/j.mfst.1673-9078.2018.12.013
    [Abstract](490) [HTML](231) [PDF 808.50 K](717)
    Abstract:
    The effects of wheat bran particle sizes on the rheological property of whole wheat dough and buns quality with or without the addition of complex enzymes (Glucose oxidase+Pentosanase+Cellulase) were studied. The whole wheat flour was prepared by adding the pretreated bran of different sizes (D50=285.0, 186.7, 75.7 μm) back to the refined flour, then the thermomechanical, alveograph, and fermentation properties of the dough, as well as buns quality, were measured. The results indicated that with the decrease of bran size, the water absorption of whole wheat flour increased; the stabilization time, tenacity, and fermentability of dough were all decreased, but the dough malleability was increased significantly. The effects of bran size on the enzymes-treated dough were quite the same as their influences on the dough in the absence of enzymes. However, the addition of enzymes decreased the stabilization time of dough, whereas the malleability, expandability, and fermentability were significantly improved. With the use of enzymes, the sensory score of whole wheat buns was highly increased. The whole wheat buns prepared with medium bran size (D50=186.7 μm) presented better sensory quality in the systems either with or without enzymes, and fine bran caused a significant decrease in buns quality.
    14  Effect of Phase Transition of Water Molecule on the Structure of Starch Gel of Huaqiao Chestnut
    CHEN Shuai ZHOU Wen-hua PAN Zhao YAN Shu
    2018, 34(12):89-95. DOI: 10.13982/j.mfst.1673-9078.2018.12.014
    [Abstract](499) [HTML](268) [PDF 7.55 M](813)
    Abstract:
    The phase transition of water molecules usually leads to the deteriorated quality of starch-based products, which is largely associated with the change in starch gel properties caused by the phase transition of water molecules. Starch-based foods often undergo the phase transitions of water molecules during freeze-thaw process. In this paper, the effects of the number of water phase transformation treatments on the microstructure and crystal characteristics of Huaqiao chestnut starch gel were studied using SEM, XRD and FT-IR. The results indicated that the phase transition of water molecules resulted in dehydration and shrinking of the starch gel structure of Huaqiao Chestnut. As the number of phase transitions increased, the pore size in the gel structure became bigger and structural rearrangements occurred. The phase transition of water molecules did not contribute to the formation of new crystal structures, mainly of CB type. With the increase of the number of phase transformation treatments, the degree of crystallinity increased from 2.97% to 12.78%. And after 7 cycles of transition phases, the crystallinity decreased to 5.71%. With the increase in numbers of transition phase cycle, a red-shift for hydroxyl groups (-OH) occurred in the FT-IR profiles; while for alcohol hydroxyl and the C-O in the C-O-C, no shifts occurred after 3-4 cycles of phase transitions, but the peak intensities increased.
    15  Effects of Different Hoarding Stages on Lipid Content and Fatty Acids of Female Chinese Mitten Crab Body Meat
    PENG Jing-wen ZHANG Yan-xia CONG Jiao-jiao WANG Xi-chang
    2018, 34(12):96-103. DOI: 10.13982/j.mfst.1673-9078.2018.12.015
    [Abstract](460) [HTML](212) [PDF 1.89 M](671)
    Abstract:
    Three sizes of female Eriocheir sinensis were studied [ first-grade crab (120±5.00 g), second-grade crab (100±5.00 g), and third-grade crab (80±5.00 g) ]. The body meat lipid content and fatty acid composition of Eriocheir sinensis in different hoarding stages were studied by Soxhlet extraction method and gas chromatography. The results showed that the body meat lipid content of the three sizes of Chinese mitten crabs in the hoarding stages was lower than that of the control group. In the maintenance stage, the lipid content of body meat in the third-grade crab decreased; the second-grade crab lipid content decreased first and then increased, and reached the maximum in the third stage; the crab lipid content in the first-stage decreased first and then rose, and is the biggest in the second stage. The three sizes of Chinese mitten crabs were the lowest in the fourth stage (the second and third-grade crabs (14~22 species); the first-grade crabs (10~23 species)). The saturated fatty acids (SFA) were mainly palmitic acid (C16:0) and stearic acid (C18:0); the highest content of monounsaturated fatty acids (MUFA) was oleic acid (C18:1), followed by palmitoleicacid (C16:1); the fatty acid content of C18:2ω6, C20:5ω3 (EPA) and C22:6ω3 (DHA) in polyunsaturated fatty acids (PUFA) was dominant. The ω3/ω6 ratio recommended by FAO/WHO and EPA+DHA value are used as evaluation indexes. The three sizes of Chinese mitten crabs body meat are most suitable for consumption in the second stage, and the first-grade crab is more beneficial to human health. Taking the content of oleic acid as the nutritional quality evaluation index, the Chinese mitten crab in the second stage has good nutritional quality.
    16  Effect of Zingibain and Actinidin on Protein Degradation of Dry-cured Mutton Ham
    AERZUGULI Abu-duwaili YSUFU Su-laiman BATUER Abuli-kemu
    2018, 34(12):104-110. DOI: 10.13982/j.mfst.1673-9078.2018.12.016
    [Abstract](473) [HTML](297) [PDF 1.56 M](645)
    Abstract:
    In order to promote the degradation of muscle protein of dry-cured mutton ham, accelerate the process of air-drying, shorten the processing cycle, and improve the quality of mutton ham, zingibain and actinidin were added in dry-cured mutton ham. The fresh mutton hind leg meat with bone were used as experimental materials, and a control group and two test groups were designed respectively for the experiment. The control group did not take the treatment, and the experimental group added 0.05% of zingibain and actinidin respectively, and dried under the corresponding conditions; and the protein degradation indicators such as Total nitrogen (TN) contents, Non-protein nitrogen (NPN) and Proteolysis index (PI) of dry-cured mutton ham were measured. SDS-PAGE (polyacrylamide gel electrophoresis) was used to analyze the degradation of muscle protein in dry-cured mutton ham. The results showed that compared with the control group, the TN content of the actinidin treatment group and the zingibain treatment group increased by 1.2 times and 1.3 times respectively after dry ripening, the NPN content increased by 1.5 times and 1.7 times respectively, and the PI increased by 1.2 times and 1.3 times respectively; SDS-PAGE analysis showed that the protein degradation efficiency of zingibain was better than actinidin. The Proteolysis index and SDS-PAGE electrophoresis results showed that the degradation degree of muscle protein of zingibain was higher than actinidin.
    17  Postharvest Mechanical Injury-induced Senescence of Green Bell Pepper Delayed by 1-MCP Treatment
    WANG Yun-xiang LIU Yao LI Xue-song ZHANG Hui-jun ZUO Jin-hua GAO Li-pu SHI Jun-yan WANG Qing
    2018, 34(12):111-116. DOI: 10.13982/j.mfst.1673-9078.2018.12.017
    [Abstract](637) [HTML](202) [PDF 1.06 M](591)
    Abstract:
    To study the effect of 1-MCP treatment on postharvest physiology and biochemical characteristics of green bell pepper with mechanical damage, this research used 10 μL/L 1-MCP to treat the pepper fruits. The quality of bell pepper fruit, cell membrane damage, the malondialehyde (MDA) content, chlorophyll content, activated oxygen scavenging enzymes such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and ascorbate peroxidase (or APX), as well as gene expression level were examined periodically during storage. The results showed that 1-MCP treatment could effectively maintain the quality of mechanically damaged pepper fruits over the storage at 20 ℃. Compared with the control group, 1-MCP treatment at 10 μL/L delayed the degradation of chlorophyll and reduction of Vitamin C, inhibited the accumulation of MDA content, and maintained the integrity of the cell membrane of the bell pepper fruits, while increasing the activities of POD, CAT and APX and gene expression. These results incdicate that 1-MCP treatment could effectively delay the senescence of the postharvest mechanically injured green bell pepper, and maintained their storage quality and prolonged their shelf life.
    18  Effect of Plasticizers on the Preservation of Blueberries by Quaternary Ammonium Salt of Chitosan Coating
    LI Cui-ping YUAN Lei YANG Yan-cun WANG Xi ZHOU Kai-yue LIU Shuang LI Peng-cheng
    2018, 34(12):117-121. DOI: 10.13982/j.mfst.1673-9078.2018.12.018
    [Abstract](470) [HTML](211) [PDF 1.54 M](615)
    Abstract:
    In order to search the plasticizers to increase the preservation effects of quaternary ammonium salt of chitosan (HTCC) coating on blueberries, 2% glycerol, sorbitol and polyethylene glycol-6000 (PEG) were added to HTCC coating, respectively. The weight loss rate, hardness, Vc content, respiratory intensity and anthocyanin content of blueberries were detected. The results showed that the coating added with glycerol could increase the weight loss and reduce the hardness of blueberries obviously and had significant difference with other groups (p<0.05). Therefore, 2% glycerol was not suitable for adding to HTCC coating. The coating added with could reduce the weight loss and respiratory intensity of blueberries, and delay the changes of anthocyanin and total soluble solid (TSS), and had significant difference with other groups (p<0.05). At 8 d, the weight loss and respiration intensity of blueberries in the group of sorbitol addition were 80.71% and 80.16%, respectively of the ones of the group processing with the coating adding with PEG , and 81.25% and 75.47% respectively of the ones of CK. Therefore, 2% sorbitol can improve the preservation effect of HTCC, which is conducive to prolonging the shelf life of blueberries.
    19  Effect of Soaking in Aqueous Ethanol on the Storage Quality of Fresh-cut Water Chestnut
    FAN Chuan-hui CHEN Xue-ling WANG Shao-hua CAI Fang MEI Xin SHI Jian-bin SUI Yong CAI Sha HE Jian-jun
    2018, 34(12):122-128. DOI: 10.13982/j.mfst.1673-9078.2018.12.019
    [Abstract](472) [HTML](236) [PDF 1.39 M](799)
    Abstract:
    In this study, the changes in the quality of fresh-cut water chestnut caused by the soaking in aqueous ethanol were examined.The changes of polyphenol oxidase activity, respiration rate, hardness, appearance color, odor, total number of colonies and number of coliforms of fresh cut mites during storage were analyzed. The effects of the ethanol concentration on the quality of fresh-cut water chestnut was evaluated through analyzing the activity of polyphenol oxidase, respiratory rate, hardness, color, flavor, total number of colonies and the number of coliforms of the fresh-cut water chestnut. The results showed that the ethanol concentration during soaking did not cause significant changes in the polyphenol oxidase activity and hardness of fresh-cut water chestnuts, but had significant effects on their respiratory rate, flavour and color.During 1 d storage, the respiration rate of fresh-cut water chestnut increased from 74 μg/(kg·h) to 134 μg/(kg·h) when the concentration of ethanol was elevated from 15% to 75%; The respiration rate of fresh-cut water chestnut treated with ethanol exhibited a downward trend over the storage . There was no significant change in the color of fresh-cut water chestnut within the first 4 d. With the prolongation of storage time, a high concentration of ethanol (≥35%) could prevent the change in the color of fresh-cut water chestnut from white to yellow. Analysis with an electronic nose showed that the higher the concentration of ethanol, the more the methyl groups and alcohols, aldehydes, ketones and other substances formed during storage. The response values of the sensors R(6) and R(8) corresponding to the methyl groups, alcohols, aldehydes,ketones increased from 111.13 and 37.10 to 270.65 and 121.70, respectively, when fresh-cut water chestnut treated with 75% ethanol. In addition,the ethanol treatment could reduce the total number of colonies and the number of coliforms in fresh-cut water chestnut. When the concentration of ethanol increased from 15% to 35%, the soaking led to the total number of colonies and the number of coliform in fresh-cut water chestnut(stored for 13 d) decreased to a countable range. When the ethanol concentration was greater than 55%, no colonies and coliforms were detected during the storage period. Thus, the shelf life of the fresh-cut water chestnut could be prolonged if they were soaked in 35%~55% aqueous ethanol and stored at 4 ℃ for 13 d.
    20  Extraction and Antioxidant Activity of Saponins from Aralia elata by Salting-out Assisted Enzymatically Hydrolysis
    LI De-hai ZHU Xiao-ran WANG Lu WANG Zhen-yu ZHAO Nan-nan GONG Jin-hua
    2018, 34(12):129-136. DOI: 10.13982/j.mfst.1673-9078.2018.12.020
    [Abstract](524) [HTML](235) [PDF 1.54 M](730)
    Abstract:
    The aims of this study were to optimize the extraction process of the Aralia elata saponin and to evaluate its antioxidant capacity. A salting-out assisted enzymatically hydrolysis process was used to extract the saponin and optimized by response surface method. The product of solvent extraction was selected as control and the effects of this process on the antioxidant activities of the extract were investgated. The results showed that the optimum extraction conditions were as follows: pH 5.405, temperature 61.3 ℃, disodium phosphate concentration 7.74%, cellulase concentration 1.5% and duration 90 min. The productivity of Aralia elata saponin was 3.97% under these conditions. The productivity of this process significantly increased comparing with solvent extraction. Results from the antioxidant test showed that the IC50 values of products extracted by the optimized process and the solvent extraction process toward DPPH radicals were 0.342 mg/mL, 0.365 mg/mL, those toward superoxide radicals were 1.220 mg/mL, 1.432 mg/mL, respectively. The total antioxidant capacity superiority sequence was as follows: Vc > optimized process extracted product > solvent extracted product. This study provides scientific data for the efficient extraction and preliminary antioxidant function clarification of Aralia elata saponin.
    21  Screening of High-yield Nucleic Acid Saccharomyces cerevisiae Strain by Atmospheric and Room-temperature Plasma (ARTP) Technique
    LI Xiao-kun WANG Wang LIN Ying LIANG Shu-li
    2018, 34(12):137-144. DOI: 10.13982/j.mfst.1673-9078.2018.12.021
    [Abstract](659) [HTML](261) [PDF 1.74 M](848)
    Abstract:
    As an important biomolecule, ribonucleic acid (RNA) can be degraded into nucleotides, nucleosides and bases which exhibit a great potential in wide applications. Saccharomyces cerevisiae is widely used in food industry and generally regarded as safe are the preferred source of nucleic acid. In this study, the cells were repeatedly exposed under atmospheric and room temperature plasma (ARTP), with the aim to screen high-yield nucleic acid strains with potassium chloride sensitivity. A mutant strain Y17aM3 was successfully obtained. In shake flask test with molasses as carbon source, Y17aM3 contained 112 mg-RNA/g-DCW RNA, 39% higher than that of the untreated control. The optimal inoculation quantity of Y17aM3 to produce RNA was 10%.The optimal pH and temperature of fermentation was 5.5 and 26 ℃, respectively. When culturing with optimal condition, it was found that adding phosphoric acid increased RNA content to 119 mg-RNA/g-DCW, and adding peptone increased RNA content to 122 mg-RNA/g-DCW. The results not only demonstrate the effectiveness of using ARTP mutation strategy for the industrial microorganism breeding, but also can greatly reduce the production cost of nucleotide-based food additives.
    22  Effects of Aroma-Producing Yeast on the Flavor Compositions of High-salt Diluted-state Broken Coix Seed Sauce
    MOU Can-can LU Hong-mei CHEN Li CHANG Dong-mei ZHANG Li
    2018, 34(12):145-152. DOI: 10.13982/j.mfst.1673-9078.2018.12.022
    [Abstract](456) [HTML](204) [PDF 1.72 M](693)
    Abstract:
    On the basis of the feasibility of using broken coix seed in the place of wheat bran for soy sauce brewing in the early stage, the effects of the addition method for aroma-producing yeast on the flavor composition of broken coix seed soy sauce were investigated by adding Saccharomyces cerevisiae (T-yeast) and Saccharomyces rouxi (S-yeast) to the sauce mash. The contents of reducing sugar, total acid, amino acid nitrogen and amino acids as well as the type and amount of volatile components resulted from different treatments were compared. When T-yeast and S-yeast were added at 2×106/g sauce on the 15th and 45th day of fermentation, the contents of reducing sugar, total acid, amino acid nitrogen and total amino acids in the sauce increased by 2.8%, 14.63%, 2.41% and 60.88%, respectively, and the types and contents of esters and alcohols in soy sauce mash were also improved significantly as compared with the blank group. The results showed that adding T yeast and S yeast to the high-salt diluted-state broken coix seed sauce mash benefited the improvement of flavor composition of the broken coix seed soy sauce, and made the sauce and ester scents of the broken coix seed soy sauce more concentrated and the flavor more mellow.
    23  Preparation and Physicochemical Properties of Starch Spherulites
    XIE Fang YANG Yin-zhou ZHANG Bin WANG Zhi-gang HUANG Qiang
    2018, 34(12):153-158. DOI: 10.13982/j.mfst.1673-9078.2018.12.023
    [Abstract](381) [HTML](210) [PDF 1.57 M](766)
    Abstract:
    In this study, waxy maize starch was used as the experimental raw material, and starch spherulites were prepared via isoamylase-catalyzed debranching and recrystallization. The effects of enzyme quantity and recrystallization time on the properties of starch spherulites were investigated though measuring the chain length distribution, branching degree, yield, morphology, crystallinity and thermodynamic properties. Results showed that the gelatinized waxy maize starch could be completely debranched by isoamylase (25 U/g) through adding the enzyme after being cooled to 50 ℃ followed by an incubation at 50 ℃ for 24 h. The average chain length of the obtained starch spherulites was 26.70. The debranched starch was recrystallized at 25 ℃ to form starch spherulites. The yield and crystallinity of starch spherulites increased with the crystallization time, which were 76.58% and 51.1%, respectively, after crystallization for 24 h. The onset gelatinization temperature (To), peak temperature (Tp), conclusion temperature (Tc) and enthalpy change (△H) of starch spherulites were higher than those for the original waxy maize starch, and these values increased with the crystallization time. The SEM images showed that the granules of starch spherulites and agglomeration of starch granules became obvious with the increase of crystallization time.
    24  Effects of Roasting on the Bioactive Contents and Antioxidant Activity of Korean Pine (Pinus koraiensis) Seed Coat
    GUO Qi-yong ZHANG Yan SHI Mao-jun ZHANG Qi-yao WU Cong ZHAO Yu-hong
    2018, 34(12):159-166. DOI: 10.13982/j.mfst.1673-9078.2018.12.024
    [Abstract](412) [HTML](222) [PDF 1.16 M](547)
    Abstract:
    The red pine seed coat contains bioactive components such as polyphenols and flavonoids, and the roasting procedure for processing the pine seed will affect the bioactive contents and antioxidant activity in pine nut seed coat. In this study, both shelled and in-shell pine seed were roasted at 130 ℃ for 10, 20, 30, and 40 min, before the total phenolic content, total flavonoid content, colour change, and antioxidant activity of the seed coat were determined The results showed that the contents of total polyphenols and total flavonoids after roasting were both lowered. The antioxidant ability of the coat of the shelled seeds was higher than that for the in-shell ones. The DPPH scavenging capacity of the shelled seed coat reached the highest after 20-min roasting, with an IC50 of 0.16 mg/mL (which was lower than that for the unroasted ones, 0.17 mg/mL). The hydroxyl free radical scavenging capacity of the shelled roasted seed coat reached the highest after 20 min, with an IC50 of 0.10 mg/mL (which was lower than that for the non-roasted ones, 0.14 mg/mL). The L* value, a value and b value all decreased with the roasting time. The total polyphenol content and total flavonoid content were positively correlated with the DPPH scavenging capacity and hydroxyl radical scavenging capacity, with the correlation coefficients as 0.799 and 0.648, respectively. The peak absorption for the seed coat extract occurred between 274 and 279 nm. Thus, roasting influences the bioactive contents, antioxidant activity and color of the pine seed coat.
    25  Effects of Fermentation Conditions on Content of Isobutanol, Isoamyl Alcohol and Phenylethyl Alcohol in Apple Brandy
    ZENG Chao-zhen KANG San-jiang ZHANG Ji-hong ZHANG Fang ZHANG Hai-yan YUAN Jing
    2018, 34(12):167-174. DOI: 10.13982/j.mfst.1673-9078.2018.12.025
    [Abstract](459) [HTML](173) [PDF 1.80 M](690)
    Abstract:
    The effects of yeast species, fermentation temperature, inoculation concentration, assimilable nitrogen and carbon source on content of isobutanol, isoamyl alcohol and phenylethyl alcohol were analyzed by headspace solid-phase micro-extraction gas chromatography-mass spectrometry (HS-SPME-GC-MS).The results showed that the contents of isobutanol, isoamyl alcohol and phenylethyl alcohol in apple brandy were relatively low with Saccharomyces cerevisiae (CICC 32130), fermentation temperature at 20 ℃, inoculation concentration of 1×104 cfu/mL in apple brandy brewing process. Assimilable nitrogen is closely related to the formation of higher-alcohols, which could help reduce the contents of isobutanol, isoamyl alcohol and phenylethyl alcohol in the suitable concentration. Otherwise, the corresponding higher alcohols are produced when adding large amount of one amino acid. However, in order to improve the alcoholicity of apple brandy, excessive sugar is added that it is not only hinder the normal metabolism of yeast, but also lead to abnormal fermentation of yeast due to the relative lack of other nutrients, which can further lead to the increase of isobutanol, isoamyl alcohol and phenylethanol in apple brandy.
    26  Effects of Oak Barrel Agingon the Quality of Kiwi Wine
    ZONG Xu-yan LI Jian BAI Bin-yang YANG Jin-shan LI Li
    2018, 34(12):175-180. DOI: 10.13982/j.mfst.1673-9078.2018.12.026
    [Abstract](483) [HTML](201) [PDF 656.04 K](574)
    Abstract:
    Oak barrel was used to improve the quality of kiwi wine during storage in this work. The changes in dissolved oxygen, electrical conductivity, chromaticity, hue, free sulfur dioxide, total phenol, and high alcohols during the aging process were measured. The sensory evaluation scores of kiwi wine before and after storage were compared. The results showed that with the prolongation of storage time, electrical conductivity, chromaticity, and hue showed an upward trend. The values of Electrical conductivity, chromaticity, and hue reached 2.76 mS/cm, 0.375, and 4.05, respectively. All of dissolved oxygen, free sulfur dioxide and total phenol showed a downward trend. The values of dissolved oxygen, free sulfur dioxide, and total phenol reached 0.72 mg/L, 27.87 mg/L, and 332 mg/L, respectively. Content of isobutyl alcohol, isoamyl alcohol and higher alcohols decreased. The sensory scores of kiwi wine after the aging in oak barrels was 97.68, which were significantly higher than that aging in glass bottle with the value 79.84. Thus, these results indicated that the attributes of kiwi wine was related to the content and change of dissolved oxygen, and the structure and composition of oak barrels.
    27  Isolation, Identification and Screening of Oleaginous Microalgae
    TU Ze-min WU Fang-yan LUO Jian-fei LIN Wei-tie
    2018, 34(12):181-186. DOI: 10.13982/j.mfst.1673-9078.2018.12.027
    [Abstract](503) [HTML](254) [PDF 1.50 M](544)
    Abstract:
    Microalgae oil is an important raw material for biodiesel production, and also one of the raw materials for the production of unsaturated fatty acids. In order to explore the potential of the oleaginous microalgae for future research and applications, we collected water samples from the East Lake of the South China University of Technology in Guangzhou and studied the growth and oil accumulation characteristics of microalgae. A total of six green algae were obtained. The identification of 18S rDNA suggested that microalgae DH1, DH2 and DH6 belong to the genus Chlorella, while strains DH3, DH4 and DH5 belong to the genus Scenedesmus. After analysis, the phylogenetic tree of six microalgal strains was constructed. The determination of intracellular oil content revealed that microalgae DH1、DH2 and DH6, especially DH2 and DH6 could accumulate oil very well. After 25 days of cultivation, the maximum biomass (cell number) of DH2 and DH6 reached 2.14 ×107/mL and 2.74×107/mL, respectively, and the oil yield reached 103.89 mg/L and 131.69 mg/L, respectively, as well as the total lipid contents were all both over 30%. These results suggest that microalgal strains DH2 and DH6 are potentially the ideal oleaginous microalgae which can be used for biodiesel production.
    28  Optimization of the Vacuum Fried Potato Chips Pretreatment Technique
    WANG Hui CHEN Zhong-ai CHEN CHAO-jun CHEN CHAO LIU JIA-liang WANG Mei LIU Hui
    2018, 34(12):187-195. DOI: 10.13982/j.mfst.1673-9078.2018.12.028
    [Abstract](581) [HTML](257) [PDF 2.48 M](782)
    Abstract:
    In order to optimize pretreatment technology of the vacuum fried potato chips, principal component analysis was performed on the experimental data obtained by single factor and response surface tests, including crushing force, oil content, L* value, sensory score, comprehensive score and electronic nose-detected volatile components. The results showed that different pretreatment methods had significant effects on crushing force, sensory evaluation and comprehensive score for potato chip (p<0.05). Principal component analysis showed that 5 principal components were extracted, and the total contribution of the first three principal components was more than 85%. It showed that the three principal components could reflect the quality information of potato chips comprehensively. The first principal component is mainly the sensory evaluation and the comprehensive score, and the second principal component is the crushing force and the oil content; and the third principal component is the L* value and the oil content.The regression effect of the quadratic polynomial regression model established by the comprehensive score in principal component analysis was extremely significant (p<0.01, R2=0.9604). The optimum process parameters were determined as the blanching temperature of 91 ℃, the blanching time of 4 min, the slice thickness of 4 mm and the freezing time of 3 h. Under these conditions, the normalized comprehensive score was 0.9572, which was close to the predicted value (0.9453), indicating that the normalized comprehensive score obtained by principal component analysis has a good predictive ability for the regression model.
    29  Effects of Ultrasonic and Osmotic Pretreatment on Quality and Antioxidant Activity of Dried Strawberry Slices
    ZHANG Li-hui LIAO Li WANG Chao AN Ke-jing QIAO Yu
    2018, 34(12):196-203. DOI: 10.13982/j.mfst.1673-9078.2018.12.029
    [Abstract](593) [HTML](239) [PDF 1.49 M](725)
    Abstract:
    The effects of sucrose, fructose, glucose and sucrose combined with ultrasonic osmotic pretreatment on the quality and antioxidant activity of vacuum freezing combined with hot air drying strawberry slices were studied. The results showed that the quality, anthocyanin, total phenol, flavonoids, VC content and antioxidant capacity of dried strawberry slices were significantly different after pretreatment (p<0.05). The water content of sucrose-ultrasonic pretreatment was the lowest, and the hardness was the highest. The color of dried strawberry slices with the highest a* was the closest to that of fresh strawberry. The contents of anthocyanins, total phenols and flavonoids in dried strawberry slices were higher than that in fresh samples, but the content of Vc was greatly reduced. The contents of anthocyanins, total phenols and flavonoids in dried strawberry slices pretreated by sucrose combined with ultrasound were 22.68 g/L, 7.52 mg/g and 10.38 mg/g, respectively. The scavenging rate of -OH and DPPH were also higher than that in other groups, but the permeation rate was higher. Ultrasound and Vc content of strawberry slices could not be improved, and the three kinds of sugar treatment could improve the quality of dried strawberry slices, and the sucrose treatment was the best. In conclusion, the quality and antioxidant activity of strawberry slices pretreated by osmosis and sucrose combined with ultrasound were higher, which was suitable for the drying of strawberry slices.
    30  Detection of Plant Oil Adulteration with Restaurant Waste-derived Lipids Based on Isoflavonoids Markers
    WU Rao DOU Xin-jing ZHANG Liang-xiao MA Fei WANG Xiu-pin DING Xiao-xia ZHANG Qi LI Pei-wu
    2018, 34(12):204-210. DOI: 10.13982/j.mfst.1673-9078.2018.12.030
    [Abstract](527) [HTML](183) [PDF 767.48 K](702)
    Abstract:
    By analyzing the sources and distribution of waste oil using isoflavonoids in soybean oil as potential markers, the thermal stability of isoflavonoids markers and their distribution in edible vegetable oils and restaurant waste oils were investigated using magnetic solid phase extraction and liquid chromatography tandem mass spectrometry. The results of the simulated repeated heating experiments showed that the markers were still detectable after the 14-h continuous heating, which indicated their reasonably good thermal stability. The analyses of the contents of isoflavonoids markers in sesame oils, rapeseed oils, camellia oils, peanut oils, flaxseed oils, soybean oils and restaurant waste oils showed that six clusters could be separated by hierarchical cluster analysis. The results showed that4 isoflavonoids including daidzein, genistein, daidzin and genistin co-existed in soybean oils, while only daidzein, genistein and a small amount of genistin were detected in the restaurant waste oils (with no daidzin). Other edible vegetable oils do not contain or do not simultaneously contain all these soy isoflavones, thus, restaurant waste oil can be distinguished from other edible oils based on the contents of the isoflavones. These results indicate that daidzein and genistein can be selected as markers for restaurant waste oils. Taking sesame oil as an example, the characteristic peaks of daidzein and genistein could be clearly observed in the chromatogram of sesame oil adulterated with 5% restaurant waste oils. Therefore, this method is reliable and effective, and could provide reference for the detection of restaurant waste oils and the market supervision.
    31  Identification of Organic Milk Powder Using Carbon and Nitrogen Natural Isotopes
    ZHAO Chao-min WANG Min ZHANG Run-he DENG Xiao-jun FANG Ke-yan
    2018, 34(12):211-215. DOI: 10.13982/j.mfst.1673-9078.2018.12.031
    [Abstract](454) [HTML](230) [PDF 372.23 K](595)
    Abstract:
    In this study, the research objects were organic milk powder and convention milk powder. The carbon stable isotope ratio and nitrogen stable isotope ratio of organic milk powder and convention milk powder were studied. Stable isotopes of carbon and nitrogen were analyzed to record the difference between convention milk powder and organic milk powder. The results showed that the overall trend of the mean δ13C value of organic milk powder was more negative than that of conventional milk powder, and the mean δ15N value of organic milk powder was lower than that of conventional milk powder. Although the data range of δ13C and δ15N values of samples were crossed, both δ13C and δ15N values were statistically different for the organic milk powder and conventional milk powder (p<0.05). The different in δ13C and δ15N values between organic milk powder and convention milk powder could be caused by the dairy cow’s diet, fertilizer types and soil characteristics used for milk production. The results of the present study, which is based on preliminary data from a limited sample size, could be highly valuable and helpful for monitoring the authenticity of organic milk powder.
    32  Development of a Droplet Digital PCR for the Detection of Haemophilus parasuis
    SHI Lei WEN Wen LI Li-li
    2018, 34(12):216-220. DOI: 10.13982/j.mfst.1673-9078.2018.12.032
    [Abstract](500) [HTML](193) [PDF 931.83 K](739)
    Abstract:
    Specific primers and one TaqMan probes were designed according to the conserved sequence of the OMP P2 gene of Haemophilus parasuis (HPS) in this study. An accurate quantitative droplet digital PCR (ddPCR) method was developed through the optimization of reaction conditions, specificity, sensitivity and reproducibility tests, analysis of clinical samples, and comparison with the real-time quantitative PCR (qPCR). Under the optimized reaction conditions, the results showed that ddPCR exhibited no cross-reactions with Actinobacillus pleuropneumoniae, Streptococcus suis, Bacillus subtilis, Escherichia coli, Salmonella, Staphylococcus aureu, Porcine circovirus 2, and swine fever virus. The developed method had a higher sensitivity than qPCR with a correlation coefficient (R2) of 0.996, a good linearity, detection limit of positive plasmid as 2.647 copies/μL, and coefficient of variation as 3.29%. The quantitative analysis results of clinical samples showed that ddPCR possessed advantages of high sensitivity and good specificity with a detection rate slightly higher than that for qPCR. The ddPCR developed in this study can accurately and quantitatively detect HPS, which will provide a useful reference for HPS related research.
    33  Residue and Dissipation of Spirotetramat and Its Metabolites in Kiwifruits and Soil
    HUANG Yu-nan QIAO Cheng-kui PANG Tao LUO Jing PANG Rong-li GUO Lin-lin LI Jun WANG Rui-ping XIE Han-zhong
    2018, 34(12):221-227. DOI: 10.13982/j.mfst.1673-9078.2018.12.033
    [Abstract](473) [HTML](182) [PDF 515.23 K](780)
    Abstract:
    This paper developed a rapid method for analyzing the spirotetramat and its four major metabolites residues in kiwifruits and soil. Samples were extracted with acetonitrile, cleaned up by Solid Phase Extraction PSA and detected by using high performance liquid chromatography-tandem mass spectrometry. Average recoveries of spirotetramat and its four major metabolites fortified at 0.01~2.00 mg/kg in kiwifruits and soil ranged from 73.97%~110.52% with the relative standard deviations (RSD) of 1.96%~7.91%, respectively. Using the above method, residue dissipation of spirotetramat and its metabolites in kiwifruits and soil were detected. After spirotetramat is applied to the soil and kiwi, it was rapidly degraded as B-enol, B-keto. In the kiwifruit, the spirotetramat and B-enol gradually decreased, while the B-keto and B-mono showed a gradual increase. In the soil, spirotetramat, B-enol and B-keto showed a decreasing trend, and B-mono and B-glu were not detected in the samples. The degradation rates of spirotetramat and B-enol in soil (1.42 d and 1.99 d, respectively) were faster than those on kiwifruit (4.08 d and 6.39 d, respectively). Sprayed twice with 3000 times liquid dilution, after 28 days, the residual amount of spirotetramat in kiwifruit was 0.06 mg/kg, which was higher than the maximum residue limit of 0.02 mg/kg formulated in China, the safe use of kiwifruit requires further study.
    34  Comparative Analysis Flavor Components of Dry-cured Ducks from Different Regions by HPLC, GC-MS Combined with Multivariate Statistical Analysis
    TONG Hong-gan WANG Wu ZHANG Hua-feng LI Pei-jun CHEN Cong-gui CHEN Jing
    2018, 34(12):228-238. DOI: 10.13982/j.mfst.1673-9078.2018.12.034
    [Abstract](515) [HTML](191) [PDF 942.48 K](762)
    Abstract:
    The aim of this study was to characterize the flavor components of dry-cured ducks from different regions. High performance liquid chromatography and solid phase microextraction gas-chromatography-mass spectrometry (SPME-GC-MS) were used to analyze the inosinic acid content and volatile constituents of six dry-cured ducks, and multivariate statistical methods were used to analyze flavor compounds comprehensively. The results showed that there were significant differences in inosinic acid content, among which the inosinic acid content of Nanan duck was the highest and the content of Yangzhou duck was relatively low content. SPME-GC-MS detected a total of 109 flavors compounds, and the aldehydes were the most abundant substances. OAV analysis showed that 11 key volatile flavor compounds were identified, of which benzaldehyde and (E,E)-2,4-nonadienal were the common key flavor substances; hexanal, nonanal, (E)-2-octenal, (Z)-2-Heptenal, naphthalene, (E)-2-decenal, 1-octen-3-ol, 2-pentylfuran, and linalool were the main substances causing the difference in flavor of dry-cured ducks from different regions. The cumulative contribution rate of the three components extracted by principal component analysis reached 85.017%, which was obvious to the dry-cured ducks from different regions. The aldehydes, inosines and esters contributed the most on PC1, which were the main flavor compounds of the dry-cured duck products. Cluster analysis also divided the dry-cured ducks from different regions into three categories, and the distinction was obvious. It can be seen that the dry-cured ducks from different regions have their own characteristics due to changes in flavor substances and the types and contents of key volatile flavor substances.
    35  Application of Digital PCR and Real-time PCR on Screening Detection of GM Components of Soybean Milk
    WANG Ming-qiu ZHANG Tao LIN Jin YANG Shuo LIU Yan LI Shi-yao MA Yi ZHANG Li
    2018, 34(12):239-245. DOI: 10.13982/j.mfst.1673-9078.2018.12.035
    [Abstract](557) [HTML](198) [PDF 1004.21 K](617)
    Abstract:
    In order to solve the implementation problems of current GM food standards and improve the inspection and supervision of GM foods in China, the application of digital PCR (dPCR) in the screening detection of genetically modified organisms (GMOs) was developed. In this study, the transgenic promoter CaMV35s and the terminator NOS genes were selected as targets and soybean endogenous gene Lectin as internal standard, and the detection limit were determined using transgenic soybean standard on real-time PCR (RT-PCR) and ddPCR systems. Two methods were both applied to GM screening detection for 30 batched of soybean milk. The results showed that the concentration detection limit of ddPCR reached 0.04 ng/reaction, and the limit of content detection reached 0.05%, which were both superior to RT-PCR (0.2 ng/reaction, 1%). In addition, 0.05% was lower than the most stringent labeling threshold level 0.9% of the European Union (EU). In the practical application of soybean milk, 26 batches of samples were consistently negative and 1 was positive on both of the two platforms, while 3 batches of samples with Ct values between 34.59~38.28 were confirmed by ddPCR, indicating that dPCR could help confirm the uncertain results of RT-PCR and slove the current standard judgment problems.
    36  Study on Amygdalin and Prunasin of Amygdalus pedunculata Pall Kernel and Meal by HPLC
    GUO Mi-mi ZHU Lin DUAN Zhang-qun ZHANG Dong LI Xiu-juan LI Xiao-ning
    2018, 34(12):246-251. DOI: 10.13982/j.mfst.1673-9078.2018.12.036
    [Abstract](509) [HTML](292) [PDF 619.77 K](673)
    Abstract:
    To clear hazardous substances from degradation products more quickly and effectively in Amygdalus pedunculata Pall meal and its by-products, high performance liquid chromatography (HPLC) was established, the amygdalin and prunasin could be determined simultaneously. When methanol was used to extract amygdalin and prunasin in Amygdalus pedunculata Pall kernel and Amygdalus pedunculata Pall meal, the suitable determination conditions were as follows: Agilent ZORBAX SB-C18 column (5 μm, 4.6 mm×250 mm), mobile phase was 20% methanol and 80% water, the flow rate was 1.00 mL/min, the detection wavelength was 210 nm, column temperature 30 ℃. The peaks of amygdalin and prunasin were isolated well; the linear range for amygdalin was 0.50~300 μg/mL (R2=0.9999), while the prunasin was 0.30~100 μg/mL (R2=0.9999); the average recovery of amygdalin of Amygdalus pedunculata Pall kernel and meal were 87.10%~96.19% (RSD=4.64%~7.83%), 98.32%~107.99% (RSD=1.44%~3.36%), respectively; while the average recovery of prunasin were 88.65%~103.10% (RSD=3.03%~7.55%), 107.58%~117.60% (RSD=1.45%~2.26%), respectively. The method is accurate and reliable, and can be used in the determination of amygdalin and prunasin in Amygdalus pedunculata Pall kernel and meal.
    37  Toxicological Safety Evaluation of the Powered Flower of Bombax malabaricum
    FENG Ding-shan LIN Wei-hua HUANG Ye-yu WU Ai-qin ZHANG Xiao-xin WANG Zhan SU Lin-liang
    2018, 34(12):252-257. DOI: 10.13982/j.mfst.1673-9078.2018.12.037
    [Abstract](511) [HTML](377) [PDF 3.28 M](896)
    Abstract:
    To assess the safety of Morinda citrifolia for long-term oral administration and provide the basis of the toxicological safety for future intake. A 90-day feeding trial was conducted using the acute toxicity test,the Ames test, the micronucleus test of bone marrow cells in mice and the sperm shape abnornality test of the national food safety standard. The maximal tolerated dose (MTD) for oral administration of powdered Bombax flower by the male and female mice were>80.00 g/kg body weight (BW). The results of the three genetic tests, the Ames test,the micronecleus test of bone marrow cells in mice and the sperm shape abnormality test in mice, were all negative. After 90 days of feeding, the food intake and food utilization rate of the high-dose group different from the normal control group, but no significant difference was detected in other indices; the low and medium dose group of SD rats caused insignificant differences in the body weight, food intake, food utilization rate,hematology, blood biochemistry, organ weight and relative ratio of organ to body from the normal control group. No significant changes in biological meaningfulness were found in the major organ coefficients and histopathological examinations. The results obtained in the current research showed that long-term intake of kapok is safe for humans in a dose of less than 40.0 g/kg BW.
    38  Studies on Volatile Aroma Compounds of the Juice of Purple Passiflora edulis and Aroma Simulation Experiments
    CHEN Qing JIA Qiang DONG Hao ZHANG Ting LI Chao
    2018, 34(12):258-263. DOI: 10.13982/j.mfst.1673-9078.2018.12.038
    [Abstract](563) [HTML](190) [PDF 469.07 K](625)
    Abstract:
    The purple red passion fruit has a pleasant aroma. In the present study, the volatile aroma compounds of the juice of purple Passiflora edulis (FJPE) were identified using headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS). In addition, the aroma of FJPE was studied through aroma simulation experiments. The results showed that 81 volatile compounds were identified in FJPE, mainly including 43 esters, 11 alcohols, 3 aldehydes, 8 ketones, 12 terpenes and 4 other compounds, and among which esters were the most abundant compounds and the major contributors to the characteristic aroma of FJPE. The simulated flavor formulation of FJPE was successfully prepared by aroma simulation experiments. The sensory evaluation revealed the rich flavour, and the naturally transitional and layered head fragrance, body fragrance and tail fragrance, which were basically consistent with the natural aroma of FJPE. In summary, it was feasible to prepare a new type of passion fruit juice flavor for food or pharmaceutical industries, with the aid of volatile compound analysis and aroma simulation technology.
    39  The Research Progress of Magnetic Relaxation Switch Technology in Biological Detection Applications
    ZHANG Jin-sheng WU Bin ZOU Deng-chao JIN Ling HU Li-wen
    2018, 34(12):264-271. DOI: 10.13982/j.mfst.1673-9078.2018.12.039
    [Abstract](601) [HTML](240) [PDF 706.41 K](794)
    Abstract:
    The research progress of magnetic relaxation switch technology in biological detection applications was introduced in this paper.The magnetic relaxation switch detection technology is a rapid detection technology with high sensitivity, good specificity and strong anti-interference ability combing nuclear magnetic resonance technology, nanotechnology with immunoassay technology. The magnetic nanoparticles modified with the biological ligand are used as magnetic resonance probe, which can identify a substance specifically.When specifically combined with the analyte,themagnetic nanoparticles change from dispersed state to aggregated state, the transverse relaxation time (T2) of the Water proton is changed to realize the detection of the analyte.At present, magnetic relaxation switch detection technology has been widely used in the detection of biomolecules such as proteins, microorganisms, nucleic acids, and small molecules, and has made some achievements.In addition, the magnetic relaxation switch detection technology based on magnetic signal has strong anti-interference ability and is suitable for complex component detection,which makes the technology has a broad application prospect in food safety, environmental detection, clinical diagnosis and other fields.

    Editor in chief:李琳

    Inauguration:现代食品科技

    International standard number:ISSN 1673-9078

    Unified domestic issue:CN 44-1620/TS

    Domestic postal code:46-349

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