Volume 34,Issue 11,2018 Table of Contents

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  • 1  6-shogaol Induces the HT29 Apoptosis and Its Relationship with VEGFR2 Expression in Human Colon Cancer Cell
    WANG Yu-feng SUN You LI Hai YANG Chun
    2018, 34(11):1-7. DOI: 10.13982/j.mfst.1673-9078.2018.11.001
    [Abstract](765) [HTML](175) [PDF 728.59 K](643)
    Abstract:
    In order to unravel the mechanism of the inhibition of colorectal cancer by 6-shogoal, the apoptosis of colon cancer cells HT29 induced by different concentrations of 6-shogoal, as well as its relationship with different expression of VEGFR2 (vascular endothelial cell receptor2) was investigated. The HT29 cells were treated with different concentrations of 6-shogoal (0, 10, 20 and 40 μM) cultured in vitro. The cell morphology changes were observed by the contrast inverted fluorescence microscope, and the cell inhibition rate was determined by CCK8 method. Annexin V-FITC/ PI flow cytometry was used to detect the changes of apoptosis rate and apoptosis cycle. Finally, the expression of VEGFR2 protein was detected by Western-blot. The results showed that as compared with the control group, 6-shogoal could induce HT29 apoptosis in a concentration dependent manner. The number of cell cycle G0/G1 cells decreased from 49.48% to 24.39% and 17.18%, while the number of G2/M cells increased from 3.44% to 34.78% and 49.54%.Thus,the cell cycle was blocked in G2/M stage to inhibit the cell proliferation. Besides, it also inhibited the expression of VEGFR2 to different extents (p<0.05).Therefore, it can be concluded that 6-shogoal can induce HT29 apoptosis and inhibit the proliferation of tumor cells, which may be related to different degrees of inhibition of VEGFR2.
    2  Inhibition of Lipopolysaccharide-induced RAW264.7 Cells Inflammatory Reaction by Total Anthocyanins from Red Cabbage
    TAN Bing PANG Qi-qi QIAN Bo WANG Cheng-qiang ZENG Zhen ZHOU Yan-yuan SONG Jia-le
    2018, 34(11):8-14. DOI: 10.13982/j.mfst.1673-9078.2018.11.002
    [Abstract](648) [HTML](211) [PDF 894.57 K](653)
    Abstract:
    The effects of total anthocyanins from red cabbage (RCTA, 1 μg/mL-50 μg/mL) on lipopolysaccharide (LPS, 1 μg/mL) induced inflammatory reaction in RAW264.7 cells were investigated. The cellular levels of nitric oxide (NO) and prostaglandin E2 (PGE2) were determined by commercial kits. Tumor necrosis factor-α (TNF-α), interlukin (IL)-1β, IL-6 and IL-8 were determined by commercial enzyme linked immunosorbent assay (ELISA) kits according the manuscripts. The mRNA expressions of induced nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and inflammatory cytokines related TNF-α, IL-1β, IL-6 and IL-8 were determined by quantitative real time polymerase chain reaction (qRT-PCR) assay. The results indicated that, RCTA significantly reduced the LPS induced secretion of inflammatory mediators (NO and PGE2) and inflammatory cytokines (TNF-α、IL-1β、IL-6 and IL-8) in RAW264.7 cells. The high concentration of RCTA (50 μg/mL) was effectively reduced the mRNA expressions of iNOS (65.80%) and COX-2 (48.28%), and generations of (58.81%) and PGE2 (46.26%) in LPS treated RAW264.7 cells. In addition, RCTA treatment was able to reduce the mRNA expressions of iNOS, COX-2, TNF-α, IL-1β, IL-6 and IL-8, respectively. The results demonstrated that the RCTA exhibited a protective effect to reduce the secretion of inflammatory mediators and cytokines.
    3  The Protection of the CCl4-induced Acute Liver Injury in Mice by Lilium Lancifolium Cyanidin
    XIAO Jing PENG An-lin
    2018, 34(11):15-20. DOI: 10.13982/j.mfst.1673-9078.2018.11.003
    [Abstract](511) [HTML](185) [PDF 795.24 K](625)
    Abstract:
    The protective effects of Lilium lancifolium cyanidin on the acute liver injury in mice caused by CCl4 were investigated. Fifty mice were randomly divided into blank control group (S), CCl4 group (M), CCl4 + Lilium lancifolium cyanidin low (25 mg/kg, LLC), medium (50 mg/kg, MLC), high (100 mg/kg, HLC) dose groups. Drug groups were given different dose drugs for days. After 2 h in last day, model was developed with the intraperitoneal injection of 0.3 mL 1% CCl4 peanut oil solution. Then, all mice were executed after 24 h, and the serum was collected for the determination of ALT, AST and ALP levels. Liver was weighed and triturated for detecting SOD, CAT and MDA. HE staining was also examined. Western blot was employed to examine the expression of Nrf2、HO-1 and NQO1 protein in the liver tissue. Compared with M group, our experimental results showed that Lilium lancifolium cyanidin could significantly reduce the levels of ALT, AST and ALP in serum, increase SOD and CAT enzyme activity of liver tissue and reduce MDA content (p<0.05). At the same time, the Nrf2、HO-1 and NQO1 protein expression of the liver tissue also significantly raised (p<0.05). The results suggest that, Lilium lancifolium cyanidin exhibited a protection against liver damage, and the underlying mechanism may be associated with its mediation of the oxidative stress reaction, antioxidant activities (e.g., scavenging free radical activity, and inhibiting lipid peroxidation activity), and the protection of liver cell membrane structure and function through the Nrf2/OH-1/ NQO1 signaling pathways.
    4  Regulation of Substrate Selectivity of a Lipase from Thermomyces lanuginosus by C-terminus Modification
    TAN Xi-yu ZHAO Ze-xin YANG Bo WANG Yong-hua
    2018, 34(11):21-27. DOI: 10.13982/j.mfst.1673-9078.2018.11.004
    [Abstract](757) [HTML](224) [PDF 918.55 K](776)
    Abstract:
    In this study, the C-terminus (I241-P256) of Thermomyces lanuginosus lipase (TLL) was replaced by the corresponding region of Aspergillus oryzae lipase (Y246-H266) through site-directed substitution, and then the mutant of TLL, named TLL_CC, was successfully expressed in P. pastoris strain X-33. The hydrolysis experiments of emulsified olive oil indicated that the substitution for C-terminus of TLL led to a reduction of 15 ℃ in optimum reaction temperature but didn’t change the pH-depedent profile of the activity, implying that long C-terminus may increase the overall flexibility of enzymes and reduce the optimum reaction temperature. Hydrolysis experiments of triolein showed that the TAG substrate remained 28.31% and 48.12% in reaction systems of TLL lipase and mutant TLL_CC, respectively. On the other hand, esterification reactions showed that the yield of TAG produced by TLL_CC was 56.67% of TLL, while the same amount of DAG was obtained. The results indicated that the substitution of C-terminus of TLL distinctly decreased its ability to hydrolyze or produce TAG, but did not affect its ability to synthesize DAG.. Thus, the substitution of amino acids on C-terminus may play important role in regulating substrate selectivity of TLL.
    5  Construction of an rahD Gene Konckout Mutant of Cholesterol-lowering Lactobacillus plantarum Lp10
    GUO Tian-Fen LIN Jun-Fang GUO Li-Qiong YE Zhi-Wei WEI Tao XU Zhi-Heng
    2018, 34(11):28-34. DOI: 10.13982/j.mfst.1673-9078.2018.11.005
    [Abstract](850) [HTML](189) [PDF 838.47 K](705)
    Abstract:
    Based on the analysis of transcriptome data, it was speculated that rhaD gene in Lactobacillus plantarum was involved in metabolism of rhamnose metabolism responsible for the biosynthesis of exopolysaccharide (EPS). As a result, the cholesterol-lowering effect of L. plantarum is realized by the adsorption of EPS whose biosynthesis was regulated by the expression of rhaD gene. In order to verify the function of rhaD gene, rhaD gene knockout vector of L. plantarum Lp10 was constructed by homologous recombination technology, the knockout vector pNZ5319-rhaD was transformed into L. plantarum Lp10 competent cells by the method of electroporation. Resistance plate was used to screen the transformants followed by the performances of PCR identification and DNA sequencing using the genome DNA as template. Results showed that the knockout vector pNZ5319-rhaD was constructed successfully, and the DNA sequencing results indicated that rhaD gene was successfully knocked out, and the cholesterol lowering ability of knockout strain increased slightly. This research not only provides technical support for the construction of L. plantarum gene knockout vectors, but also lays the theoretical foundation for the subsequent functional verification of genes related to the cholesterol-lowering effects of L. plantarum.
    6  Study on the Lipid-lowering Effect of Xiang Qi Lower Blood Lipids Chewable Tablet
    LU Min CHANG Xiao-jie ZHANG Wen-jin LIU Ning YANG Jia-mei LIU Yan-hua MA Xue-qin YANG Jian-hong YU Jian-qiang LI Feng-mei
    2018, 34(11):35-40. DOI: 10.13982/j.mfst.1673-9078.2018.11.006
    [Abstract](542) [HTML](209) [PDF 4.92 M](740)
    Abstract:
    The effects of Xiang Qi lower blood lipids chewable tablet on body weight, blood lipids and liver function in hyperlipidemia model mice were investigated in this work. Sixty male Institute of Cancer Research mouse were randomly divided into 6 groups: normal group (normal saline), model group (normal saline), simvastatin-positive group (5 mg/kg), and Xiang Qi lower blood lipids chewable tablet high dose (2 g/kg), medium dose (1 g/kg) and low dose (0.5 g/kg) groups. The normal group was fed with normal feed, and the other groups were fed high-sugar and high-fat feed. Oral administration during the experiment, and various experimental indicators were measured after 6 weeks. The hyperlipidemia model obtained with this modeling method has little effect on the body weight of mouse. Compared with model group, total cholesterol, low-density lipoprotein, aspartate aminotransferase and alanine aminotransferase of the hyperlipidemia mouse serum of decreased Xiang Qi lower blood lipids chewable tablet groups with the largest reduce rates of 28.85%, 37.90%, 25.08% and 42.09%, while high-density lipoprotein increased with the highest growing rate of 73.70%. The results demonstrated that Xiang Qi lower blood lipids chewable tablet has the function of hypolipidemicing and protecting liver, and this effect is most obvious in high dose (2 g/kg).
    7  Inhibition of Ethanol-induced Hepatic Steatosis in Human HL7702 Liver Cells by Four Constituents Isolated from Hovenia Acerba
    DU Rui-xue WANG Rui LI Shan-ji
    2018, 34(11):41-45. DOI: 10.13982/j.mfst.1673-9078.2018.11.007
    [Abstract](547) [HTML](179) [PDF 472.75 K](596)
    Abstract:
    To discover the effective substances in Hovenia acerba capable of dispeling the effects of alcohol and protecting the liver, this study examined the effects of four constituents in the ethyl acetate extract from Hovenia acerba on hepatic steatosis. The cells metabolic activity/viability of the control group, model group, or high-/medium-/low-dose group of the four bioactives was measured by the MTT assay. The lipid droplets in the cells were observed by Oil red O staining after ethanol modeling. The levels of cell TG and liver enzymes, ALT and AST, were determined. The optimal concentration of ethanol-induced cells was measured by the MTT assay. It was found that compared with the control group, the cell viability decreased after of 0.9% ethanol modeling for 48 h, along with the observations that Oil red O-stained lipid droplets became more obvious in the cells and the cells became swollen and round. after the exposure of ethanol-treated model cells to each of the four bioactives, a slight increase in the survival rate of the cells was induced by dihydromyricetin and quercetin as compared with the model group; compared with the control group, the TG content and the leakage of ALT and AST of the model group significantly increased (p<0.05). Further, compared with the model group, the application of 0.9% ethanol combined with dihydromyricetin or quercetin on the normal human HL7702 hepatocytes for 48 h caused a significant (p<0.05) decrease in the TG and ALT, AST contents in the liver. Accordingly, the active ingredients in the ethyl acetate phase obtained from Hovenia acerba that were capable of dispeling the effects of alcohol and protecting the liver were mainly dihydromyricetin and quercetin, especially the former.
    8  Controlled Bioactive Compound Delivery Systems for Liquid Food and Their Release Behaviors
    XU Han-shan ZHENG Bo LI Lin CHEN Ling
    2018, 34(11):46-51. DOI: 10.13982/j.mfst.1673-9078.2018.11.008
    [Abstract](538) [HTML](224) [PDF 1007.87 K](738)
    Abstract:
    The storage stability and controlled release of bioactive compounds were achieved through microencapsulation controlled release delivery system. The film-coated microparticle delivery systems for liquid food based on resistant starch acetate (RSA) were established by the methods of extrusion-spheronisation and fluid-bed spray coating. The releasing properties during yoghurt storage and in vitro simulated gastrointestinal tract environment of RSA-coated microparticles with different thickness were investigated. Results showed that under the same storage time, the larger coating thickness was, the more intact coating layer would be, which could prevent the release of bioactive compounds. When the thickness of the film-coated is 9.94%, the bioactive compounds did not release during storage for 22 days. Additionally, after storage in yogurt, the change of the surface morphology of microparticle with different coating thickness in the simulated gastrointestinal tract environment was consistent with their release. The results provide a theoretical basis and basic data for screening suitable carrier materials for bioactive compounds and its target-controlled delivery system for liquid foods.
    9  Inhibition of Bel-7402 Human Hepatoma Cells and H22 Tumor-bearing Mice by Maca Alkaloids
    WANG Ai-hua LIU Ying-mei WANG Li-li AN Ran
    2018, 34(11):52-56. DOI: 10.13982/j.mfst.1673-9078.2018.11.009
    [Abstract](541) [HTML](240) [PDF 29.69 M](880)
    Abstract:
    The effects of maca alkaloid on the anti-hepatocarcinoma activity of Bel-7402 cells in vitro and the anti-H22 liver cancer activity in vivo were investigated. Maca alkaloid crude extract was obtained by 0.5% hydrochloric acid and 95% ethanol solvents, and maca alkaloid was further purified by acid dissolution and alkali precipitation method. The anti-hepatocarcinoma activity of maca alkaloids was determined by MTT method and the anti-H22 liver cancer activity in vivo. The cell experiments showed that maca alkaloids of different doses exhibited an anti-liver cancer activity towards Bel-7402 cell, and the decreasing order of the activity was maca alkaloids high-dose group > maca alkaloids middle-dose group > maca alkaloids low-dose group. The in vivo anti-liver cancer results showed that maca alkaloids at high or middle doses had a significant anti-liver cancer activity. The results demonstrated that maca alkaloids exhibit an anti-liver cancer activity, which would provide a new direction for the study of maca alkaloid activity.
    10  Stability of High Protein Food System Constructed with Soy Protein Particles
    ZHANG Tao GUO Jian YANG Xiao-quan HE Man-yuan WANG Zi-xu HUANG Shu-yan
    2018, 34(11):57-64. DOI: 10.13982/j.mfst.1673-9078.2018.11.010
    [Abstract](670) [HTML](217) [PDF 1.17 M](647)
    Abstract:
    Soy protein isolate (SPI) was subjected to shear and heat treatment under acidic conditions (pH 3.0, 3.4 and 3.8), and protein particles distributed from 2 to 100 μm were obtained. As prepared at pH condition was more close to the isoelectric point of SPI, the resulting particles had a higher degree of microparticulation and a better stability. For the soy protein particles prepared at pH 3.8, their internal protein content was 34.22%, and their protein leakage did not exceed 13% within the range of pH 2.0 to 8.0. The change of pH and re-heat treatment (95 ℃ for 30 min) had little effects on their particle size and morphology. Compared to SPI dispersion, the viscosity of soy protein particle dispersion decreased significantly after heat treatment. And heat-set gelation did not occur in this dispersion even when the particle concentration was 12%. These protein particles could be used in the high-protein foods such as beverage and yogurt, so as to make them have smooth mouthfeel and fortify their protein content.
    11  Antifungal Mechanisms of the Active Ingredients Carvacrol of Mosla Chinensis 'Jiangxiangru' against Penicillium Digitatum
    QI Wen-wen SHEN Yu-ting CHEN Chu-ying CHEN Jin-yin WAN Chun-peng
    2018, 34(11):65-69. DOI: 10.13982/j.mfst.1673-9078.2018.11.011
    [Abstract](610) [HTML](206) [PDF 582.02 K](615)
    Abstract:
    Penicillium digitatum was used as the test fungal species in this study to investigate the antifungal mechanism of the active ingredients of Mosla chinensis 'Jiangxiangru', namely carvacrol. Antifungal activity was evaluated by determination of the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC), measuring the antifungal activity of a series of doubling dilution carvacrol samples. The acting mechanism of carvacrol was elucidated by evaluating its effects on mycelial morphology, spore germination, cell membrane permeability, soluble sugar content. Results showed that the MIC and the MFC of carvacrol were 0.125 mg/mL and 0.25 mg/mL, respectively; the morphological changes of mycelium were appeared by treatment with different concentrations carvacrol; furthermore, a lower spore germination rate, elevated cell membrane permeability, reduced soluble sugar were also observed by treatment with different concentrations carvacrol. The antifungal activity of carvacrol may be related to inhibit spore germination in Penicillium digitatum, damage the structure of its cell membrane, facilitate leakage of intracellular contents, reduce nutrient content. Thus, carvacrol exerts an inhibitory effect by inhibiting normal growth and reproduction of mycelia.
    12  Study on the Quality of Pulse-spouted Radio Frequency Vacuum Drying of Prepared Cabbage
    WANG Yu-chuan WANG Yi-xiang WANG Bo XIAO Min XU Jing-jing
    2018, 34(11):70-76. DOI: 10.13982/j.mfst.1673-9078.2018.11.012
    [Abstract](513) [HTML](252) [PDF 916.53 K](707)
    Abstract:
    In order to solve the problems of poor quality, high cost, high labor intensity and high pollution of added high-sugar vegetables dried by traditional vacuum drying, in this paper, the quality and uniformity of dried cabbages pretreated with added sugar were studied using pulse-spouted radio frequency vacuum drying (PSRFVD). Results showed that the ascorbic acid content, chlorophyll content, reducing sugar content, rehydration ratio, moisture content, color and shrinkage ratio of PSRFVD cabbages pretreated with heating blanching (24.41 mg/100 g, 3.12 mg/100 g, 61.41%, 26.11, 94.51%, 96.31% and 92.11%, respectively) were higher than those pretreated with hot water blanching (21.68 mg/100 g, 2.98 mg/100 g, 58.11%, 21.19, 93.21%, 95.63% and 91.45%, respectively). Compared to VD, PSRFVD not only effectively solved the caking problem, but also improved the quality of the dried products, reducing the drying time by>50%.
    13  Heat Resistance and Identification of Characteristic Strain of Thermophilic Bacteria from Epidermal Mucus of White Mullet Fish (Opniocepnalus argus var. kimnra)
    ZHANG Long-yi ZHANG Yin CHEN Ping-ping CHEN Ting-ting FANG Jian-yu WANG Lin-guo KE Huan XIONG Wei
    2018, 34(11):77-82. DOI: 10.13982/j.mfst.1673-9078.2018.11.013
    [Abstract](747) [HTML](252) [PDF 674.04 K](547)
    Abstract:
    To deeply analyze the heat resistance of the characteristic microbes and identifying the characteristic strain in the epidermal mucus of white mullet fish, the carcass mucus of white mullet was heat treated, then the characteristic bacteria in the heat treated mucus were isolated and purified, and their species were identified. By comparing the appearance characteristics and heat resistance of the heated mucus, and morphological features of the characteristic bacteria, the growth curve of the characteristic bacteria and the heat resistance of the strain S3 were investigated. Results showed that the fresh mucus was faint red. The mucus was gradually became light green with the increase of heat temperature. Three kinds of colonies, namely S1, S2, S3, were observed in the culture medium inoculated with the 30 ℃, and S2 and S3 in the culture medium inoculated with the 50 ℃, while only S3 in the culture medium inoculated with the 70 ℃, 90 ℃, boiling treated mucus. No colony was found in the control medium. Further analysis of S1, S2 and S3 bacteria showed that S1 was gram-negative bacterial strains S2 and S3 were gram-positive bacterial strains. The strain S2 and S3 reached the peak of growth when they were cultured for 14h and 16h, respectively. By analyzing 16S rDNA sequence of S1, S2 and S3, it was found that strain S1 belonged to Acinetobacter sp., strain S2 belonged to Kocuriarhizophila sp., and strain S3 belonged to Bacillus sp. Therefore, the thermophilic bacterium inepidermal mucus of white mullet fish is Bacillus subtilis.
    14  A Kinetic Study on the In Vitro Simulated Digestion of Gummy Vitamin C and Calcium Candies
    XU Xiao-fei ZHONG Hao-quan LIU-Wei XIA Wan-xia YANG Ji-guo
    2018, 34(11):83-88. DOI: 10.13982/j.mfst.1673-9078.2018.11.014
    [Abstract](727) [HTML](211) [PDF 932.68 K](725)
    Abstract:
    An in vitro simulated digestion model was used to study the release kinetics of the functional components, calcium and vitamin C, from their gummy candy vehicles. The microstructure of gummy candies was characterized, and the effect of dietary components on the release of functional components of the gummy candy in a simulated gastric juice was investigated. The results showed that the disintegration time of the gummy calcium and vitamin C candy was 155 min and 22 min, respectively. The functional component, Vitamin C, was released gradually and reached up to 93.6% in 2 h, and the calcium component was released almost 100% within 30 min. Both gummy candies exhibited a basic colloidal network structure, but differed in the morphology of network structure. The release kinetics of vitamin C was best matched by Higuchi's fitting effect, which was consistent with the release law of sustained-release preparation, whilst the gummy calcium candy was best fitted with the first-order kinetics. In addition, it was found that the gastric juice containing 5% starch slowed down the release of the functional component, Vitamin C, from the gummy candy, but other dietary ingredients had no significant effect on its release. The digestion and release characteristics of the gummy calcium candy in simulated gastric juice were not affected by the dietary ingredients. Results of this study indicate that compared with tablets and hard capsules, the disintegration time and release rate upon digestion of the gummy candies were slower and exhibited a somewhat sustained-release effect.
    15  Inhibitory Effect of Artemisia Lavandulaefolia Extract on Staphylococcus Aureus
    MAO Gen-nian HU Jia-huan LIU Yi-xiu
    2018, 34(11):89-94. DOI: 10.13982/j.mfst.1673-9078.2018.11.015
    [Abstract](651) [HTML](272) [PDF 553.64 K](671)
    Abstract:
    The ultrasonic-assisted extraction with 95% ethanol was used to generate a crude extract from Artemisia lavandulaefolia. Then the dried crude extract paste was treated sequentially with petroleum ether, ethyl acetate and n-butanol to obtain different extraction fractions. Steam distillation was used to extract the volatile oil from Artemisia lavandulaefolia. The in vitro antibacterial activity of each fraction of the crude extract and the volatile oil against Staphylococcus aureus was examined using the filter paper dilution system and double diluting method. The effect of Artemisia vlavandulaefolia extract on the growth curve of Staphylococcus aureus was studied. The in vivo antibacterial activity was investigated using Kunming mice. In vitro experiments revealed that the antibacterial ability of each fraction of the crude extract or volatile oil followed this order: ethyl acetate phase > n-butanol phase > petroleum ether phase = volatile oil. No antibacterial activity was detected for the aqueous fraction. The minimum inhibitory concentrations (MICs) of the petroleum ether phase, ethyl acetate phase, n-butanol phase, and volatile oil were 10.00, 2.50, 5.00, and 10.00 mg/mL respectively. The ethyl acetate and n-butanol fractions exhibited significant inhibitory effects on the growth of Staphylococcus aureus (p<0.05). The results of in vivo experiments showed that the highest in vivo antibacterial activity was detected when the mice were fed 0.50 g/kg of wild Artemisia lavandulaefolia extract, with the mortality rate being 77.78% lower than the model group. Thus, the extract of Artemisia lavandulaefolia has antibacterial activity against Staphylococcus aureus.
    16  Relationship between 1-MCP Treatment and Antioxidant Enzyme Activities and Quality of Korla Fragrant Pear during Room-Temperature Storage
    DU Lin-xiao ZHAO Xiao-min LI Dan MA Nan LI Xue-wen
    2018, 34(11):95-102. DOI: 10.13982/j.mfst.1673-9078.2018.11.016
    [Abstract](727) [HTML](176) [PDF 827.28 K](643)
    Abstract:
    Using Korla fragrant pear as raw material, 1.0 μL/L of 1-methylcyclopropene (1-MCP) treatment was used under the condition of 25 ℃ for fumigation processing, with distilled water as control group. After fragrant pear fruits were stored under the normal temperature library (22 ℃~25 ℃、RH 80%~85%), the fruit enzymes CAT (catalase), POD (peroxidase) and SOD (superoxide dismutase) activity, Hue-angle, firmness, respiration rate, cell membrane permeability, MDA (malondialdehyde) content, greasiness level and wax content were measured every 5 days. The results showed that 1-MCP treatment could maintain high CAT, POD and SOD activity of pear fruits compared with control; slow down the decrease of fruits Hue-angle and firmness; delay the increase of fruits respiration rate, cell membrane permeability and malondialdehyde content, inhibit the increase of greasiness level and wax content. The greasiness level of the 1-MCP treatment group and the control group showed a significant positive correlation with the wax content, and the correlation coefficient was 0.948** and 0.898**, respectively. The correlation coefficient between CAT activity and respiration rate of 1-MCP treatment group and control group was 0.846* and 0.832*, respectively. 1-MCP treatment can maintain the activity of antioxidant enzymes in fruits, maintain the quality of fruits, and delay the process of fruits senescence.
    17  Changes in the Contents of Bioactive Substances and Activities of Antioxidant Enzymes during the Development of Mulberry Fruit
    LIU He-ping ZHANG Guang-ming HU Hui-gang HUANG Xin-liang YANG Zhuan-ying
    2018, 34(11):103-110. DOI: 10.13982/j.mfst.1673-9078.2018.11.017
    [Abstract](506) [HTML](246) [PDF 1.20 M](698)
    Abstract:
    The changes in the contents of bioactive substances and the activities of antioxidant enzymes during the development of mulberry fruit were researched. The 3-year-old and 5-year-old mulberry fruits were used as the materials for the studies on the changes of the total phenolics, total flavonoids, phenolic acids, tannins, chlorophyll and carotenoids, anthocyanins, soluble sugar, total antioxidant capacity and antioxidant enzymes’ activities. The obtained results showed that the total phenolics, total flavonoids, phenolic acids, tannins, anthocyanins and soluble sugar contents increased during the fruit development, while chlorophyll a decreased gradually. The contents of chlorophyll b and carotenoids increased firstly and then decreased during the fruit development. The 3-year-old fruit had higher contents of total flavonoids and phenolic acids but lower contents of total phenolics, tannins and carotenoids than the 5-year-old fruit. The activities of superoxide dismutase (SOD), catalase (CAT) and phenylalnine ammonialyase (PAL) in the fruit flesh decreased firstly and then increased. The activities of peroxidase (POD) and ascorbate peroxidase (APX) decreased gradually whilst the total antioxidant capacity increased gradually. Throughout the development period, the 3-year-old fruit had higher activities of SOD, CAT and PAL but lower APX activity and total antioxidant capacities, compared to the 5-year-old fruit. Therefore, the changes in the contents of bioactive substances and the activities of antioxidant enzymes were closely related to fruit maturity and tree age. During the development of mulberry fruit, the total antioxidant capacity of fruit flesh increased continuously and reached the highest on the 25th day after flowering. The older the mulberry tree, the higher the total antioxidant capacity of fruit was.
    18  Quality Changes and Analysis of Volatile Compounds of Lonicera caerulea L. during Fermentation
    FENG Jun-ye LI Jia-qi WANG Ping
    2018, 34(11):111-118. DOI: 10.13982/j.mfst.1673-9078.2018.11.018
    [Abstract](796) [HTML](177) [PDF 884.57 K](707)
    Abstract:
    In this paper, the quality changes of Lactobacillus plantarum fermented honeysuckle (Lonicera caerulea L.) pulp and juice were studied. The changes of pH, viable cell counts, total phenolic content, anthocyanin content, hydroxyl radical scavenging ability, DPPH free radical scavenging ability, SOD activity and amylase activity were investigated during 48 h fermentation. The volatile compounds of honeysuckle pulp and juice at 48 h of fermentation were analyzed by gas chromatography-mass spectrometry (GC-MS). The results showed that through fermentation, the quality changes of the honeysuckle pulp and juice were as follows: the pH decreased significantly (p<0.05), and finally was 3.71 and 3.51, respectively. The number of Lactobacillus plantarum was 7.42 Log CFU/mL and 7.85 Log CFU/mL at 20 h and 24 h,respectively. The total phenolic content showed the highest value at 40 h, which was (0.89±0.06) mg/mL and (0.70±0.02) mg/mL, respectively.The anthocyanin content was significantly reduced (p<0.05). The hydroxyl radical scavenging ability reached the highest point at 40 h, which was 83.12±3.59% and 80.60±2.87%, respectively. DPPH free radical scavenging ability did not change significantly (p>0.05). The SOD activity reached a maximum at 36h, which was (45.59±1.07) U/mL and (49.59±0.71) U/mL, respectively. The amylase activity achieved the peak at 48 h,which was (8.77±0.37) U/mL and (11.93±0.57) U/mL, respectively. By GC-MS analysis, 86 and 70 volatile compounds were detected in the fermented honeysuckle pulp and juice, respectively. Ethyl decanoate (34.42%) and ethyl octanoate (21.37%) are the main volatile compounds in the fermented honeysuckle pulp; and ethyl octanoate (20.67%), ethyl 9-decenoate (17.82%) and ethyl decanoate (15.51%) are the main volatile compounds in fermented honeysuckle juice. Ethyl decanoate is a characteristic flavor compound in fermented honeysuckle.
    19  In Vitro Antioxidant Activity and Preservative Effect of Longan Seed Essential Oil
    CAI Wen-tao YUAN Xue-wen CHEN Qiu-yuan YU Wei-ye FENG Li-na
    2018, 34(11):119-125. DOI: 10.13982/j.mfst.1673-9078.2018.11.019
    [Abstract](575) [HTML](259) [PDF 32.63 M](676)
    Abstract:
    This study investigated the in vitro antioxidant activity and preservative effect at ambient temperature of Longan seed essential oil. Longan seed essential oil was extracted with organic solvent n-hexane for the determination of 1, 1-diphenyl-2-picrylyl (DPPH·) free radical,hydroxyl radicals (·OH) and sodium nitrite (NaNO2) scavenging abilities. The preservative effects were assessed based on the changes in physiological indices including Longan flesh color, peel browning index, weight loss rate, soluble solids content and titratable acidity. The results showed that the Longan seed essential oil exhibited detectable DPPH·, ·OH and NaNO2 scavenging activities, with respective IC50 values as 11.04, 4.58 and 0.12 mg/ mL, and a dose-effect relationship was found within the experimental concentration range. The essential oil can reduce peel browning index and weight loss rate of Longan peel; the change in the soluble solid content was small; the titratable acidity changed slowly in the early stage of storage, but increased rapidly in late stage. There was no significant difference (P > 0.05) between the essential oil-treated group and the control (untreated) group. The Longan seed essential oil did not exert obvious preservative effect on Longan fruit. Excessive essential oil could also cause different degrees of phytotoxicity to Longan fruit.
    20  Effects of Different Extraction Methods on the Composition and Antioxidant Activity of the Polysaccharides in Lilium davidii var. unicolor Salisb.
    HUANG Yu-long GAO Qing-ya QUAN Ting SUN Ruo-shi ZHANG Fang LI Wei-qi ZHANG Ji
    2018, 34(11):126-131. DOI: 10.13982/j.mfst.1673-9078.2018.11.020
    [Abstract](748) [HTML](200) [PDF 23.93 M](842)
    Abstract:
    Lilium davidii var. unicolor Salisb. was used as the raw material for the investigation on the effects of three extraction methods, microwave-assisted extraction, ultrasonic-assisted extraction, and ultrasonic treatment coupled with enzymatic hydrolysis, on the structure and composition of the obtained polysaccharides (named LPS-M, LPS-C, and LPS-CE, respectively). After precipitation with ethanol, deproteinization and freeze-drying, the three types of polysaccharide samples were characterized by FT- IR, and the result indicated that LPS-M, LPS-C and LPS-CE all had the characteristic absorption peaks of polysaccharides (at 3378, 2931, 1640 and 1060 cm-1). The monosaccharide compositions of LPS-M, LPS-C and LPS-CE were analysed by GC-MS,and the results showed that these polysaccharides had the same types of monosaccharides but at different molar ratios i.e. the molar ratios of the two major components, mannose and glucose, were at 5.2:4.9, 5.1:4.7, and 4.8:5.3, respectively. The analyses of the antioxidant activities by hydroxyl radicals, superoxide radicals, DPPH free radicals and reducing power assays revealed that antioxidant activity of LPS-CE was significantly higher than those of LPS-C and LPS-M. The method of ultrasonic treatment coupled with enzymatic hydrolysis could be used as the preferred extraction method for extracting functional polysaccharides from Lilium davidii var. unicolor Salisb..
    21  Extraction and Immunomodulatory Activities of Active Components from Chinese Herbs-koji
    XIONG Chuan CHEN Zu-qin WU Zheng-yun TANG Xue ZHU Yu
    2018, 34(11):132-137. DOI: 10.13982/j.mfst.1673-9078.2018.11.021
    [Abstract](750) [HTML](207) [PDF 15.65 M](622)
    Abstract:
    In order to evaluate the antioxidant and immunomodulatory activity of a traditional Chinese herbs-koji, the contents of total polyphenols, total flavonoids and polysaccharides were measured. Ultrasound-assisted extraction was used to obtain total polyphenols and total flavonoids. Polysaccharides in Chinese herbs-koji (HKP) were extrated by water extraction and alcohol precipitation. The antioxidant activity of polyphenols and flavonoids were evaluated by the use of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radicals, hydroxyl radicals, and superoxide anion radicals methods. The potency of HKP on the phagocytosis and cytokine secretion of RAW 264.7 cells was determined by commercial kit. Results showed that the content of total polyphenols in the Chinese herbs-koji was 33.54 mg/g, the total flavonoid content was 10.88 mg/g, and the polysaccharide content was 74.98 mg/g. The extracted polyphenols and flavonoids showed good free-radical scavenging capacity to DPPH radical, superoxide anion radical and hydroxyl radical. Furthermore, the corresponding half inhibitory concentration (IC50) of polyphenols is 18.87 μg/mL, 26.24 μg/mL and 33.31 μg/mL, and for flavonoids is 33.24 μg/mL, 38.97 μg/mL and 33.08 μg/mL. The HKP resulted in a significant increase of lymphocyte proliferation and could also enhance the effect of Con A-induced T lymphocyte proliferation and the stimulation index of LPS-induced B lymphocytes. In addition, the phagocytosis and cytokine secretion of RAW 264.7 cells can be promoted by HKP. The traditional Chinese herbs-koji can be developed and utilized as a health product.
    22  Purification and Characterization of the Polyphenoloxidase (PPO) from Solenocera crassicornis in the East Sea of China
    LYU Min HUANG Guang-hua YANG Hui-zan WANG Rui MA Hua-wei GAN Hui ZENG Lan XIANG Xing-wei
    2018, 34(11):138-144. DOI: 10.13982/j.mfst.1673-9078.2018.11.022
    [Abstract](669) [HTML](280) [PDF 30.54 M](683)
    Abstract:
    In this study, the polyphenoloxidase (PPO) was extracted using the homogenate extraction from Solenocera crassicornis (S. crassicornis) in the East China Sea, and purified through ammonium sulphate precipitation, ion-exchange and gel filtration. The substrate specificity (L-dihydroxyphenylalanine (L-DOPA) as the substrate) and kinetic properties of the purified PPO, along with the effects of different inhibitors and metal ions on the PPO, were determined. The obtained results showed the optimal conditions for the homogenate extraction were: the material to liquid ratio 1:2 (W/V), pH 7.5, time 3 h, and temperature 50 ℃. The yield and purification factor were 1.2% and 25.9, respectively. This PPO significantly catalyzed the oxidation of L-DOPA and catechol (p≤0.05), with the Km values being 3.8 mM and 8.12 mM, respectively, indicating that the PPO belongs to the catechol enzyme family. The PPO was sensitive to phenylthiourea, ascorbic acid, and citric acid, and its activity was strongly inhibited by Cu2+, Zn2+, and ethylenediaminetetraacetic acid (EDTA). Thus, the PPO from S. crassicornis could be a metalloenzyme containing Cu2+ in its active center. The results of this study provide the basis for controlling the melanotic blackening of S. crassicornis.
    23  Preparation of Chitosan/Agarose Two-Component Hydrogels and Its Water Holding and Sustained Release Properties
    HUANG Dong-ye ZHANG Yang LIU Hong WU Jia-jie PAN Lian-hua WU Yan ZHU Hai-bo TU Tao
    2018, 34(11):145-150. DOI: 10.13982/j.mfst.1673-9078.2018.11.023
    [Abstract](707) [HTML](231) [PDF 24.91 M](882)
    Abstract:
    Novel chitosan/agarose two-component hydrogels were successfully prepared using chitosan and agarose in the presence of 1% citric acid and through a heating-cooling process. The resulting two-component hydrogels were characterized through the determination of phase transition temperature, scanning electron microscopy (SEM) analysis, transmission electron microscopy (TEM) analysis and rheological measurements. The obtained results showed that a lower proportion of chitosan and higher gelator concentration resulted in hydrogels with a higher phase transition temperature and higher stability. SEM and TEM analyses revealed that the hydrogels had a loose porous structure. Then, the hydrogels were freeze-dried and ground into powder, and such a xerogel powder was made into granules by a granulation technique. In a high temperature and dry environment, the water adsorbed by the xerogel granules was gradually released into the environment, thereby exhibiting a sustained and slow moisturizing effect, which has a good application prospect in keeping packaged food moisturized and improving food taste. The water retention and moisturizing mechanisms of xerogel granules were proposed based on the water vapor adsorption experiment sand thermogravimetric analysis.
    24  Application of Artificial Neural Networks for Process Simulation and Quality Control of Dry-sausage
    XU Liang CHENG Jing-rong ZHANG Ye-hui TANG Dao-bang LIU Xue-ming LIN Yao-sheng CHEN Zhi-yi
    2018, 34(11):151-156. DOI: 10.13982/j.mfst.1673-9078.2018.11.024
    [Abstract](532) [HTML](213) [PDF 36.19 M](661)
    Abstract:
    The purpose of this study was to achieve rapid detection and evaluation of the quality of air-dried sausage under natural conditions. In this paper, different processing conditions (such as wind speed, temperature, humidity) were used through simulating the natural conditions, and the resulting physicochemical indices, lipid oxidation and protein oxidation of dried sausage were compared. Then an artificial neural network modelling method was used to construct a neural network model for predicting and simulating the processing conditions for dry sausage production. The obtained results showed that sausages with low fat and high protein (protein content 38.65% and fat content 16.06%) were obtained under the following processing conditions: wind speed 0.80 m/s, daytime temperature 23.60 ℃, daytime humidity 40.42%, night-time temperature 16.89 ℃, and night-time humidity 54.21%. The established artificial model r values above 0.99 and rmse values below 0.4, indicating that the model can make highly accurate predictions and can predict the optimal sausage processing conditions. Therefore, this method can describe the effect of processing conditions on the quality of air-dried sausage and provide a theoretical basis for the establishment of an artificial climate and quality and safety control for large-scale processing of air-dried sausage.
    25  High-speed Shear-Ultrasonic Combination Extraction of Hypoglycemic Components from the Fruits of Viburnum sargentii Koehne
    FU Qun WANG Meng-LI LI Hui WU Tong
    2018, 34(11):157-164. DOI: 10.13982/j.mfst.1673-9078.2018.11.025
    [Abstract](747) [HTML](255) [PDF 58.55 M](842)
    Abstract:
    In this paper, α-glucosidase and α-amylase inhibition rate were used as indicators to optimize the process of high-speed shear-ultrasonic combination with ethanol assisted extraction of hypoglycemic components from the fruits of Viburnum sargentii Koehne. Regression coefficient and analysis showed that the regression model fitted the actual process well. Based on the results of response surface methodology, the best extraction process parameters were ascertained as follows: the high speed shear dispersing emulsifier’ speed is 18000 r/min, the solid-liquid ratio is 1:19 (g/mL), the extraction temperature is 60 ℃, the ultrasonic time is 40 min, shear cutting time is 120 s, ultrasonic power is 270 W. Under these conditions, the comprehensive inhibition rate was (65.89±1.03)%, the α-glucosidase inhibition rate was (72.11±0.86)%, and the α-amylase inhibition rate was (56.57±1.05)%.The IC50 were 0.844 mg/mL, and 1.422 mg/mL respectively. Comparing enzyme inhibition rate, high-speed shear-ultrasonic combination was 11.39% (p<0.01) higher than the ultrasound method and 13.64% (p<0.01) higher than the high-speed shear method.
    26  Process Optimization of ACE Inhibitory Peptides from Scallop Skirt Fermented by Bacillus natto
    GAO Jie GAO Xiang YU jia WEI Yu-xi JIAO Kui ZHANG Xue-mei WANG Jin-mei LI Yu-jin
    2018, 34(11):165-175. DOI: 10.13982/j.mfst.1673-9078.2018.11.026
    [Abstract](717) [HTML](160) [PDF 165.83 M](590)
    Abstract:
    In order to develop safe and food-derived angiotensin converting enzyme (ACE) inhibitory peptides, the scallop skirt was fermented by Bacillus natto. The single factor test was used to determine the fermentation paremeters such as fermentation time, fermentation temperature, inoculum size and liquid-to-material ratio with the peptide content and ACE inhibition rate as indicators. Box-Behnken response surface analysis was applied to optimize the process conditions and a quadratic multiple mathematical model was established. The results showed that the regression model could better reflect the relationship between the level of each factor and the response value, the order of factors affecting peptide content was time > liquid-to-feed ratio > inoculation amount > temperature, the influence of each factor on ACE inhibition rate was time > liquid-to-feed ratio > inoculation amount > temperature. The best technological conditions for the preparation of ACE-inhibitory peptide from natto fermented product were as follows: fermentation time 38.4 h, fermentation temperature 39.9 ℃, inoculation amount 6.0%, liquid to material ratio 22.5. Under the fermentation conditions above, the polypeptide content was 238.91 mg/g in the lyophilized product, in which the ACE inhibition rate was up to 83.70%. The results of this study provide a theoretical basis for the high-valued application of scallop skirt and development of food-derived ACE inhibitory peptides.
    27  Effects of Berry Pomace on Qualities and Antioxidant Activity of Yogurt
    HU Jin-tao KONG Fang ZOU Mu-fa ZHENG Xue ZHAO Yu-hong
    2018, 34(11):176-184. DOI: 10.13982/j.mfst.1673-9078.2018.11.027
    [Abstract](607) [HTML](217) [PDF 20.11 M](1051)
    Abstract:
    In this experiment, 1.50% of berry pomace (red raspberry, blueberry, and black currants) was added to the yoghurt product and stored at 4 ℃ for 21 days. The purpose is to study the changes of composition, quality and antioxidant capacity of yoghurt enriched with three kinds of berry pomace during storage. The results showed that the contents of total dietary fiber in red berries, blueberry and black currants were 45.24%, 50.43% and 55.21%, respectively, and the contents of polyphenol were 18.40 mg/g, 18.70 mg/g, 30.00 mg/g, respectively. The contents of flavonoids were 3.00 mg/g, 2.00 mg/g, and 2.90 mg/g, respectively. The contents of anthocyanins were 14.30 mg/g, 14.10 mg/gand 10.50 mg/g, respectively. The DPPH? scavenging capacity (the concentrations of extracts were 10.00 mg/mL) were 92.20%, 84.10%, 61.00%, respectively. Within 21 days of storage, the enrichment of berry pomace did not change the tendency of yoghurt whey precipitation rate and water holding capacity during storage, titration acidity was higher than 88.78, pH was at 4.17~4.51.The enrichment of berry pomace elevated the concentration of total dietary fiber, and yoghurt's hardness, viscosity, cohesiveness and viscosity index were also higher than control. The antioxidant properties of yoghurt were significantly increased. During the storage period, the DPPH? scavenging capacity, total reducing power, and hydroxyl radical scavenging rate were significantly increased to 3.93, 13.11 and 4.28 times, respectively, compared to the control. The addition of berry pomace could be used in yoghurt as functional ingredients.
    28  Comparison of the Quality of Litchi Wines Produced through Three Different Winemaking Processes
    ZHU Yun-ting MI Sheng-xi CAI Yong-jian ZHAO Mou-ming DENG Xin-lun ZHAO Qiang-zhong
    2018, 34(11):185-193. DOI: 10.13982/j.mfst.1673-9078.2018.11.028
    [Abstract](644) [HTML](262) [PDF 14.25 M](909)
    Abstract:
    The effects of the freeze concentration technology on the quality of Litchi wine were studied. Three different winemaking processes, direct fermentation, fermentation-freeze concentration, and freeze concentration-fermentation led to the production of fermented Litchi wine, fermented-concentrated Litchi wine and concentrated-fermented Litchi wine, respectively, from Litchi juice. Then comparative analyses of physicochemical indicators, amino acids content and volatile substances and sensory evaluation of 3 Litchi wines were conducted. The obtained results showed that the fermented Litchi wine had an alcohol content of 8.48 %, total acid of 4.80 g/L, tannin content of 0.53 g/L, total free amino acid of 40.38 mg/L and the alcohol-ester ratio of 0.86, with a light fruity smell and an ordinary mouthfeel. While the fermented-concentrated Litchi wine and the concentrated-fermented Litchi wine had 16.10% and 16.47% alcohol, 9.57 g/L and 7.70 g/L total acid, 1.02 g/L and 1.13 g/L tannin content, 102.97 mg/L and 493.17 mg/L total free amino acid, respectively, which were significantly higher than those of the fermented Litchi wine (p<0.05). The fermented-concentrated Litchi wine had an alcohol-ester ratio of 0.50 with an increase of wine aroma, but the acidity and content of undesirable flavor were high (which didn’t taste good). The concentrated-fermented Litchi wine had the alcohol-ester ratio of 0.26 with a strong fruity smell and full-bodied mouthfeel (superior to the other two Litchi wines). The study indicates the freeze concentration before fermentation could result in the production of Litchi wine of high quality.
    29  Effects of Cold Plasma Treatment on Quality of Beef
    ZHANG Jian-hao QIAO Wei-wei HUANG Ming-ming WANG Jia-mei
    2018, 34(11):194-199. DOI: 10.13982/j.mfst.1673-9078.2018.11.029
    [Abstract](640) [HTML](197) [PDF 12.83 M](777)
    Abstract:
    In order to study the effects of cold plasma treatments (modified atmospheric packaging (O2: CO2: N2=35%: 35%: 30%)) on sensory quality of beef, the pH, myoglobin, surface color, drip loss, shear force and texture changes of samples were analyzed during storage at 4 ℃ after cold plasma treatment. Results showed that during refrigerator storage, the pH of treated beef was relative stable, L* values were relative high, which were higher than that of untreated samples; a* values of treated samples were increased during 4 days then decreased, though they were lower than that of untreated samples; while the b* values were increased significantly (p<0.05) and higher than that of untreated beef; the total content of myoglobin were reduced, while the metmyoglobin amount was increased during the storage; the hardness, gumminess and chewiness significantly (p<0.05) decreased by 4.78%, 2.97% and 6.13%, respectively, while the elasticity, cohesiveness and recovery changed not significantly. These results indicated that the cold plasma treatment could enhance the brightness, reduce the redness, and increase the yellow color of beef surface, and it had no negative effects on the texture characteristics and pH of beef.
    30  Extraction Optimization through Response Surface Methodology and Antioxidant Activity of Polyphenols of Nymphaea Hybrid
    ZHUO Qi ZENG Ying ZHU Zun-ling
    2018, 34(11):200-207. DOI: 10.13982/j.mfst.1673-9078.2018.11.030
    [Abstract](687) [HTML](195) [PDF 50.30 M](845)
    Abstract:
    In order to explore the best extraction technology and antioxidant activity of polyphenols from Nymphaea hybrid, the optimization of polyphenols extraction conditions was researched by the method of response surface methodology. The influences of extraction temperature, liquid/solid ratio, extraction time, and alcohol concentration on polyphenol extraction yield were researched by single factor experiments. Based on Box-Behnken, four-factor and three-level response surface methodology was carried out to optimize the extraction technology. The antioxidant activity of polyphenols was evaluated using DPPH and ABTS radical scavenging activities and FRAP reducing power. The results found that the optimum conditions for polyphenol extraction were obtained as follows: extraction temperature of 58 ℃, liquid/solid ratio of 48:1 (mL/g), extraction time of 1.9 h, and alcohol concentration of 57%. Under the optimal conditions, extraction rate of N. hybrid polyphenols was 8.28%. The DPPH and ABTS scavenging abilities of N. hybrid polyphenols were 90.22% and 88.01%, respectively. The FRAP value was increased with the polyphenol concentration, exhibiting a dose-dependent effect. These results suggested that N. hybrid polyphenols has an obvious antioxidant activity.
    31  Evaluation of the Bacterial Diversity in Cured Fish Samples Collected from Enshi by PCR-DGGE and MiSeq High-throughput Sequencing
    WANG Yu-rong LIAO Hua ZHAO Hui-jun ZHANG Zhen-dong GUO Zhuang
    2018, 34(11):208-213. DOI: 10.13982/j.mfst.1673-9078.2018.11.031
    [Abstract](750) [HTML](239) [PDF 9.31 M](888)
    Abstract:
    Cured fish is a kind of Chinese traditional food which is popular for its unique taste and flavor.The bacterial diversity of cured fish samples collected from Enshi was studied by 16s rDNA gene-based PCR-DGGE and MiSeq high-throughput sequencing. The result of PCR-DGGE indicated that the domain bacteria in cured fish samples were belong to Psychrobacter and Lactobacillus. The result of MiSeq high-throughput sequencing indicated that Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria constituted more than 1.0% of total sequences at the phylum level, with the relative abundance of 61.29%, 30.21%, 5.34%, and1.74%, respectively. Meanwhile, Psychrobacter, Brochothrix, Pseudomonas, Staphylococcus, Acinetobacter, Vibrio, Pseudoalteromonas, and Chryseobacterium constituted more than 1.0% of total sequences at the genus level, with the relative abundance of 35.70%, 19.74%, 7.13%, 7.12%, 4.19%, 3.90%, 3.09%, and 1.98%, respectively. At the OTU level, 64 OTUs were found with the cumulative average relative was 62.67% of total qualified sequences. Thus, the main dominant bacteria in cured fish samples were composed of Psychrobacter belonging to Proteobacteria and Brochothrix belonging to Firmicutes, and all samples shared a large number of core bacterial microbiome.
    32  Non-destructive Determination for Pear Fruit Quality by DA-Meter
    GUAN Ye-qing QIN Xiao-li PEI Ying WEI Chuang-qi GUAN Jun-feng
    2018, 34(11):214-219. DOI: 10.13982/j.mfst.1673-9078.2018.11.032
    [Abstract](559) [HTML](159) [PDF 20.07 M](842)
    Abstract:
    In order to determine rapidly and non-destructively the quality of pear fruit taking advantage of an non-destructive determination equipment, DA-Meter, quality indexes of different pear cultivars (Early-maturing: “Lvbaoshi”, “Zaosu”. Mid-maturing: “Xinli No.7”, “Huangguan”, “West green”, “Xueqing”. Late-maturing: “Yuluxiang”, “Hongxiangsu”, “Yali”, “Xuehua”) fruit and index of absorbance difference (IAD) of fruit surface based on DA-Meter were determined at different maturity stage, and correlation analysis was made between IAD value and different quality indexes. The results showed that there were linear correlations between IAD value and soluble solid content (SSC), soluble sugar, titratable acid (TA) and vitamin c (Vc) contents, as well as peel saturation C value, respectively, during fruit maturation in different cultivars of pears. Furthermore, IAD value had extremely significant linear correlations with firmness, seed browning rate and peel H value of color ratio at p<0.01 level. It indicated that IAD value could effectively response to the fruit quality and maturity. Thus, the DA-Meter could be used in the postharvest classification process under non-destructive determination in pear fruits.
    33  Rapid Detection of the Number of “Golden Flower” Fungus in Fuzhuan Brick Tea Based on Image Processing Technology
    LV Jia-li MENG Yan-nan SHI Zhao-ye LUO Xiao
    2018, 34(11):220-226. DOI: 10.13982/j.mfst.1673-9078.2018.11.033
    [Abstract](543) [HTML](175) [PDF 58.69 M](727)
    Abstract:
    The quantity of “Golden Flower” Fungus is one of the important indicator for evaluating the quality of Fuzhuan brick tea. In order to quickly and accurately detect the quantity of "Golden Flower" Fungus in Fuzhuan brick tea, an automatic and rapid detection method of “Golden Flower” Fungus is designed based on that the “Golden Flower” Fungus in Fuzhuan tea is large, golden-yellow, clear and distinguishable under the naked eye. Firstly, the Fuzhuan brick tea was cut randomly, and the photographing equipment with high-resolution was used to obtain the image of the cutting surface. The Plus Image-Pro software has been employed to get the “Golden Flower” Fungus numbers of each sample automatically by image processing system and automatic counting system. The results showed that: the detection result is reliable and has no significant difference with the manual counting method. Therefore, the image processing technology can effectively and quickly detect the “Golden Flower” Fungus in the Fuzhuan brick tea, so as to quickly and accurately evaluate the quality of the Fuzhuan brick tea.
    34  Isolation of Staphylococcus aureus from Cooked Food Samples around Campus and the Detection of Staphylococcal Enterotoxin Genes
    ZENG Bao-feng JIANG Min WANG Hong-zhi CHEN Juan ZHAO Yan-ying TANG Jun-ni
    2018, 34(11):227-234. DOI: 10.13982/j.mfst.1673-9078.2018.11.034
    [Abstract](584) [HTML](162) [PDF 6.67 M](785)
    Abstract:
    The contamination of Staphylococcus aureus and the distribution of staphylococcal enterotoxin genes in cooked food around campus were investigated in this study. A total of 89 food samples were collected from food markets and roadside stalls around campus. The distribution of 21 staphylococcal enterotoxin genes was detected by polymerase chain reaction (PCR) for all S. aureus food isolates. The results showed that seventy-one S. aureus strains were isolated and identified, with the total isolation rate of 79.78%. For market samples, the isolation rate was 80.70%; for roadside food stall samples, the isolation rate was 78.12%. Among 21 staphylococcal enterotoxin genes, sec, see, seh, sel, sep and ses genes were not detected. Other 15 types of genes, including 3 classical enterotoxin genes and 12 new enterotoxin genes, were detected. Forty-six (64.78%) S. aureus isolates carried one or more enterotoxin genes. Among them, 23 strains carried two or more enterotoxin genes, accounting for 50%. The detection rates of 21 staphylococcal enterotoxin genes in 46 enterotoxigenic S. aureus food isolates were as follows: sex (86.96%); sei and sem (32.61%); sen and seo (30.43%); set (21.74%); seg (13.04%); sea、ser and seb (10.87%), sej and seu (6.52%), sek and seq (4.34%), sed (2.17%). This study showed that the isolation rates of S. aureus strains and the detection rates of staphylococcal enterotoxin genes were very high in cooked food samples around campus. The genotypes of new enterotoxin were more than those of classical enterotoxins. This study provides a reference for staphylococcal food safety.
    35  β-glucosidase Effectively Improves the Floral, Fruity and Grassy Notes of Instant Oolong Tea Infusion
    FANG Ke LI Ting ZHU Yan-bing NI Hui HUANG Gao-ling
    2018, 34(11):235-242. DOI: 10.13982/j.mfst.1673-9078.2018.11.035
    [Abstract](535) [HTML](170) [PDF 10.16 M](769)
    Abstract:
    To investigate the effect of β-glucosidase on the volatile components of instant oolong tea infusion, gas chromatography-mass spectrometry (GC-MS), aroma activity values (OAVs) and sensory evaluation were used to analyze the volatile compounds and the main aroma profiles. Totally 37 kinds of volatile compounds, including 9 alcohols, 4 esters, 11 olefins, 4 furans, 5 aldehydes, and 4 of other chemicals were identified. The content of 11 kinds of volatile components, including cis-3-hexenol (15416.97 μg/L), hexanol (476.14 μg/L), geraniol (6542.43 μg/L), benzaldehyde (707.32 μg/L), methyl salicylate (268.81 μg/L), 3-methyl butanol (95.24 μg/L), 2-heptanol (61.26 μg/L), 2-ethyl hexanol (47.61 μg/L), octanol (30.29 μg/L), decyl alcohol (103.26 μg/L), and hexyl hexanoate (87.75 μg/L) increased after the treatment with β-glucosidase. Meanwhile, the floral, fruity and grassy note were greatly enforced, and the major aroma contributor were furfural, cis-3-hexenol, geraniol, 2-methylbutanal and 2-ethylfuran. These results showed that β-glucosidase had a significant effect on the volatile compounds and aroma profile of instant oolong tea infusion, which provides a technological reference for improving the aroma quality of instant tea based foods.
    36  The Scenting Technology and Aroma Analysis by HS-SPME/GC-O-MS for Osmanthus Black Tea
    CHEN Hui-min SHI Zhi-gang DI Tai-mei HU Jian-hui ZHANG Zhong-qiang ZHANG Xin-fu
    2018, 34(11):243-254. DOI: 10.13982/j.mfst.1673-9078.2018.11.036
    [Abstract](1004) [HTML](258) [PDF 20.86 M](922)
    Abstract:
    To investigate the optimal scenting technology and the characteristic aroma components of sweet-scented osmanthus black tea, orthogonal experiments were carried out and the aroma components were analyzed by using headspace solid phase microextraction (HS-SPME) combined with Gas Chromatography-olfactometry-mass Spectrometry (GC-O-MS) technique. The results of the orthogonal test and sensory evaluation showed that the optimal scenting conditions were: The osmanthus-black tea ratio, 3:10, tanning three times with each lasting 4 hours. The GC-MS analysis revealed that the aroma components adsorbed into the sweet-scented osmanthus black tea were mainly four compounds cyclohexenol, nonyl aldehyde, γ-decalactone and 6,10,14-trimethyl-2-pentadecanone. The newly generated aroma components during the scenting process were mainly 1-octene-3-ol, 2-methyl-2-nonen-1-ol, 4-chloro-bicyclo [2.2.1] heptan-2-one, 3-methyl-2-furanmethylbutyrate, hexylpentanoate, trans-2-hexenyl pentanoate, and phenethyl butyrate. These results suggest that these 11 compounds were the main aroma components of osmanthus black tea. The GC-O analysis revealed that nonyl aldehyde exhibited oily and sweet orange odor, γ-decalactone possessed pleasant peach fragrance, 1-octene-3-ol had hay fragrance, trans-2-hexenyl pentanoate had creamy and fruity aroma, and phenethyl butyrate had honey sweet aroma. These five substances may be the characteristic aroma components responsible for the osmanthus fragrance of osmanthus black tea. In addition, the relative contents of alcohols significantly decreased while the contents of ketones and esters increased significantly during the scenting process.
    37  Simultaneous Determination of Five Estrogens in Feed by Ultra Performance Liquid Chromatography-tandem Mass Spectrometry Coupled with Solid Phase Extraction
    FANG Ke-yan ZHAO Chao-min DENG Xiao-jun CHEN Qin
    2018, 34(11):255-261. DOI: 10.13982/j.mfst.1673-9078.2018.11.037
    [Abstract](606) [HTML](194) [PDF 24.58 M](774)
    Abstract:
    A method was developed for the simultaneous determination of five kinds of estrogens (dienestrol, diethylstilbetrol, hexestrol, 17α-estradiol and 17β-estradiol) in feed by ultra performance liquid chromatography-tandem mass spectrometry coupled with solid phase extraction. Estrogens were extracted by ethyl ether from the feed sample. The extract was deproteinized with trichloroacetic acid (TCA), defatted with n-Hexane, and then purified by Max phase extraction column. Five kinds of estrogens were derived by Athena C18-WP chromatographic column with gradient elution of acetonitrile/0.25‰ ammonia water. Finally, identification and quantification were performed using multiple reaction monitoring (MRM) with one precursor ion, and two product ions for each analyte and electrospray ionization in negative mode. The parameters of the liquid chromatography and mass spectrometry were also optimized to enhance detection sensitivity. The quantity of estrongens were determined by internal standard method with 17β-estradiol-D3 and diethylstilbestrol-D8 as reference. The results showed that the linear ranged from 0 to 10 μg/L for five estrogens with the correlation coefficients (r) were no less than 0.9994. The limits of quantitation (LOQ) for all analytics were 1.0 μg/kg. At the spike levels of 1.0 μg/kg, 2.0 μg/kg and 5.0 μg/kg for each estrogen, the recoveries of estrogens were within the range of 70.20%~114.37%, and the relative standard deviation was no more than 9.08% (n=6). The results demonstrated that the developed method can simultaneously determine the five estrogens in feed with advantages of simple pretreatment and high accuracy.
    38  Comparison of 3M PetrifilmTM Rapid Test Strips and the National Standard Method for Analyzing the Mold and Yeast Species in Beverages
    ZHOU Jin-Zhen XU Hong CHEN Ling LIU Ting-Ting GUO Wei-Peng
    2018, 34(11):262-267. DOI: 10.13982/j.mfst.1673-9078.2018.11.038
    [Abstract](638) [HTML](266) [PDF 672.26 K](750)
    Abstract:
    In this study, 3M PetrifilmTM Rapid Test Strips for yeasts and molds and the national standard method were used in parallel for mold count and yeast count tests for a functional vitamin beverage, to determine whether or not the analysis results obtained by the two methods were significantly different. Five standard strains (Candida albicans, Candida tropicalis, Debaryomyces hansenii, Trichoderma virens and Aspergillus carbonarius) were used for screening. Strain preselection test, repeatability test and specificity test of the rapid test strips, and comparative tests between the rapid test strip method and the national standard method, were performed. The obtained results showed that the strains selected through the preselection test could all grow well in the test samples. Meanwhile, the repeatability test revealed that the results of the second test were within the acceptable range of those of the first test; the specificity test revealed that the rapid test strips method had high specificity. The results of the mold and yeast counts determined by the two comparative methods were statistically analyzed by the F test and t test, and it was found that there was no significant difference between the results obtained by the 3M PetrifilmTM rapid test strip method and the national standard method.
    39  Development of a Prediction Model for Evaluation of Palatability of Cooked Rice
    WANG Jing-hua XIE Zhen-hua ZHANG Po SUN Guang-mei MA Xiao-hong LI Yue-xing SUN Yi-min LIU Wei
    2018, 34(11):268-274. DOI: 10.13982/j.mfst.1673-9078.2018.11.039
    [Abstract](460) [HTML](252) [PDF 29.22 M](673)
    Abstract:
    To predict the sensory evaluation scores based on the parameters determined by a texture analyzer, the raw rice samples were prepared according to the national standard GB1354-2009, and the cooked rice samples were prepared in large amounts and evaluated according to GB/T15682-2008. In this study, 60 lots of Japonica rice (round-grained) samples and 60 lots of Indian rice (long-grained) samples were collected from different regions. The palatability of cooked rice as an objective quality index was used as the independent variable, the subjective sensory evaluation score was obtained according to the national standard GB/T15682-2008 as the dependent variable, to establish a linear model between the textural parameters and the sensory evaluation score of the same rice by the partial least squares method. There were significant differences among the different rice samples in the overall sensory evaluation scores and textural parameters. But a linear correlation between the textural parameters and the sensory evaluation scores was found for the same rice. The equation for the obtained linear regression model has a minimum fitting degree of 95.86%. Therefore, the measurements of textural parameters using the texture analyzer allow an accurate prediction of the sensory evaluation scores of cooked rice samples.
    40  Risk Assessment of Pesticide Residues to Four Nectar Honey in Northwest China
    TAN Yang SUN Xiao-feng HAN Yan GENG Gui-gong CUI Ming-ming XIAO Ming
    2018, 34(11):275-280. DOI: 10.13982/j.mfst.1673-9078.2018.11.040
    [Abstract](609) [HTML](171) [PDF 12.64 M](721)
    Abstract:
    Samples of rape honey, sunflower honey, acacia honey and medlar honey were collected in the past two years. Residues of the five pesticides (acetamiprid, imidacloprid, thiamethoxam, carbendazim, and propargite) were detected. The risk assessment of dietary honey exposure to children, gestational age Female and old man was conducted using the risk quotient RQ%. The results showed that the detection rate of the five pesticides was between 5.22% and 29.57%. With reference to the European Union's limit of pesticide residue, 13.91% of acetamiprid content was exceeded, and the over-standard rate of imidacloprid was 7.83%. The detection rate of pesticide residues in different sources of honey ranged from 25% to 100%, and the detection rate of medlar honey was the highest. ANOVA analysis of pesticide residues in honey from different honey plants revealed significant differences in the residual levels of carbendazim, acetamiprid and imidacloprid, which may be closely related to the degree of pesticide contamination in the pollen source plants. The risk quotients (RQ%) of the three groups were all less than 1%, indicating that the residual risk is low and acceptable. In different populations, children under 10 years of age have the highest dietary exposure risk.
    41  Simultaneous Determination of 14 Functional Components in Health Foods by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry
    YANG Hui-cheng WANG Hai-ming LIN Xu YAN Yun HE Xiao-feng
    2018, 34(11):281-289. DOI: 10.13982/j.mfst.1673-9078.2018.11.041
    [Abstract](705) [HTML](213) [PDF 38.67 M](1209)
    Abstract:
    The functional component and composition are commonly analyzed for health foods, and are important quality indices of these products. An ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established for simultaneous determination of 14 kinds of plant-based functional components present in health foods. Ultrasonic extraction with methanol was applied to the samples prior to analysis by UPLC-MS/MS using a Venusil MP C18 column (100 mm×2.1 mm, 3 μm) and a gradient mobile phase composed of acetonitrile and water containing 0.1% formic acid. The chromatographic peak areas were quantified using external standards. The results showed that the chromatographic peak of all the test samples were eluted within 4 min by the present UPLC-MS/MS method. Good linearity was observed in the range of 0.05~10 ng/mL for aloin and adenosine,0.5~100 ng/mL for rutin, icariin, amygdalin and paeoniflorin, and 5~1000 ng/mL for the other 8 kinds of functional components, with the correlation coef?cients greater than 0.998. The method precision, the relative standard deviation, was less than 5%. The detection limit was 75 μg/kg for chlorogenic acid, gallic acid, sodium danshensu, resveratrol, salidroside, astragaloside, glycyrrhizin and allicin, 0.75 μg/kg for aloin and adenosine, and 7.5 μg/kg for rutin, icariin, amygdalin and paeoniflorin. The recovery rate was 83.42%~108.44% based on the recoveries of the spike-and-recovery experiments at three standard concentrations. Thus, this current method had a high sensitivity, good linear relationship and reproducibility, and can be used for simultaneous determination of various functional components in a health food, which provides a technical support for the quality control of health foods.

    Editor in chief:李琳

    Inauguration:现代食品科技

    International standard number:ISSN 1673-9078

    Unified domestic issue:CN 44-1620/TS

    Domestic postal code:46-349

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