Volume 33,Issue 3,2017 Table of Contents

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  • 1  Effects of Glycine on Hepatic IGF-I Expression Regulation and the Underlying Signaling Pathways
    XU Jing-ren LIU Shi-zhong ZHANG Meng-yuan WAN Xiao-juan CAI Xing-cai WANG Li-na ZHU Xiao-tong GAO Ping WANG Song-bo JIANG Qing-yan SHU Gang
    2017, 33(3):1-7. DOI: 10.13982/j.mfst.1673-9078.2017.3.001
    [Abstract](874) [HTML](267) [PDF 827.16 K](1285)
    Abstract:
    To investigate the effects of Glycine (Gly) on the expression and secretion of hepatic insulin-like growth factor 1 (IGF-1), HepG2 cells were treated with different concentrations of Gly in vitro. Meanwhile, mice were injected (i.v.) with 1.0 g/kg Gly or equimolar alanine (Ala) via tail veins, and blood, liver, and gastrocnemius samples were collected at 0.5 h and 1 h post-injection. The expression of IGF-1 and insulin-like growth factor binding proteins (IGFBPs) mRNAs in hepatocytes and mouse liver was analyzed by quantitative polymerase chain reaction (qPCR). Meanwhile, changes in the hepatic growth hormone receptor pathway (JAK2/STAT5) and muscular IGF-I receptor pathway (ERK/Akt/mTOR) were also detected by western blot. The results showed that the expression levels of IGF-1 protein and mRNA in HepG2 cells were elevated by Gly in a dose-dependent manner. The in vivo data demonstrated that 1.0 g/kg Gly could remarkably increase the serum IGF-1 and albumin levels, significantly upregulate the expression levels of hepatic IGF-1 and IGFBP-3 mRNA, and downregulate that of IGFBP-1 mRNA. In vitro and in vivo experiments both indicated that Gly could effectively activate the JAK2/STAT5 signaling pathway in HepG2 cells and mouse liver, and enhance the phosphorylation level of the IGF-I receptor signaling pathway in gastrocnemius. These results suggest that glycine may directly improve the sensitivity of hepatic growth hormone receptor signaling pathways, thus promoting the expression and secretion of IGF-1. These results provide the experimental basis for revealing the molecular nutrition function and action mechanism of Gly.
    2  Metabolomics Study of Pinus massoniana Pollen Extract and its Preventive Effect on Benign Prostate Hypertrophy in Mice
    MA Yu YANG Chang-jun SUN Feng-xia LV Jian-yun WANG Tong GENG Yue
    2017, 33(3):8-13. DOI: 10.13982/j.mfst.1673-9078.2017.3.002
    [Abstract](1156) [HTML](218) [PDF 648.31 K](860)
    Abstract:
    The effect of masson pine (Pinus massoniana) pollen extract (MPPE) on benign prostate hypertrophy (BPH) in mice was studied. Male KM mice were randomly divided into six groups: normal control group, BPH model group, 20, 4080 mg/kg MPPE groups and 0.8 mg/(kg?d) finasteride group. After two weeks of advanced intragastric MPPE administration, an intraperitoneal injection of testosterone propionate was used to initiate the BPH, following which the MPPE or finasteride treatment was administered intragastically for 4 weeks. At the end of the treatment period, morphological changes in the tissues were observed, and levels of the cytokines IL-6, IL-1β, and TNF-α as well as of PACP, DHT, NO, and NOS were measured. Ultraperformance liquid chromatography-mass spectrometry was employed to detect changes in serum and urine metabolites. Partial least squares discriminant analysis was used for group differentiation and biomarker selection. All three doses of MPPE decreased the prostate index, inflammatory cytokines, DHT concentration, PACP and NOS activities, and NO production. Serum metabolomics analysis found and identified three potential biomarkers: 1-palmitoyl-sn-glycero-3-phosphocholine, 1-O-hexadecyl-2-O-acetyl-sn-glyceryl-3-phosphorylcholine, and cis-13-docosenoamide. MPPE could significantly reduce the pathological symptoms and biochemical parameters of BPH and adjust the level of the marker metabolites. The mechanism may be related to the inhibition of inflammation and alleviation of lipid metabolism disorders.
    3  Effect of Lactobacillus plantarum 35 on Escherichia coli-induced IL-8 Production and E. coli Adhesion on HT-29 Cells
    GUO Yuan-ji TUO Yan-feng MOU Guang-qing JIANG Shu-juan QIAN Fang LIU Peng SHEGN Xiao-fang
    2017, 33(3):14-21. DOI: 10.13982/j.mfst.1673-9078.2017.3.003
    [Abstract](890) [HTML](207) [PDF 572.04 K](1072)
    Abstract:
    The adhesive ability of eight strains of Lactobacillus plantarum on the HT-29 intestinal epithelial cell line, and the inhibitory effect of the strain with the highest adhesive ability and its exopolysaccharides (EPS) on Escherichia coli-induced IL-8 production and E. coli adhesion on HT-29 cells were investigated. The results indicated that the eight Lactobacillus strains adhered to HT-29 cells to different degrees, and L. plantarum 35 had the highest adhesive ability. L. plantarum 35 could protect HT-29 cells from E. coli adhesion by displacement, competition, and exclusion, with inhibition rates of 30%, 33%, and 59%, respectively. At an active concentration of 500 μg/mL, EPS could inhibit E. coli adhesion by 32%. L. plantarum 35 could inhibit E. coli-induced IL-8 production by HT-29 cells by displacement, competition, and exclusion with inhibition rates of 3%, 28%, and 40%, respectively. EPS could inhibit E. coli-induced IL-8 production by HT-29 cells in a dose dependent manner, with the highest inhibitory rate of 50% at 500 μg/mL concentration. L. plantarum 35 had no effect on IL-10 production by HT-29 cells. The results showed that L. plantarum 35 has a potential probiotic function and may inhibit inflammatory bowel disease induced by E. coli.
    4  Isolation and Purification of Sea Cucumber Peptide and Its Impact on the Secretion of Collagen by NIH/3T3 Cells
    SONG Shu-liang WANG Huan LIANG Hao JI Ai-guo
    2017, 33(3):22-28. DOI: 10.13982/j.mfst.1673-9078.2017.3.004
    [Abstract](931) [HTML](238) [PDF 726.47 K](1125)
    Abstract:
    A sea cucumber peptide, SP12, was isolated and purified from Stichopus japonicus, and its impact on NIH/3T3 cell proliferation and secretion of type I collagen was studied. SP12 was purified by ion exchange chromatography and gel filtration chromatography from fresh Stichopus japonicus using a cell activity tracking method. The effect of SP12 on NIH/3T3 cell proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and its impact on cell cycle progression was determined by flow cytometry. The effect of SP12 on collagen secretion by NIH/3T3 cells was measured by hydroxyproline kit, and its impact on the expression of type I collagen, matrix metalloproteinase-1 (MMP-1), and tissue inhibitor of metalloproteinases-1 (TIMP-1) in NIH/3T3 cells was measured at the protein and gene levels by western blot and reverse-transcription polymerase chain reaction (RT-PCR). The results clearly demonstrate that SP12 can considerably enhance the proliferation rate of NIH/3T3 cells and promote progression from G1 phase to S phase. In a range of 0~20 μg/mL, SP12 enhanced collagen secretion by NIH/3T3 cells in a dose-dependent manner. At the protein and genetic levels, SP12 considerably promoted the expression of type I collagen and TIMP-1, and inhibited the expression of MMP-1; this might be the mechanism underlying SP12 promotion of collagen secretion by NIH/3T3 cells.
    5  Memory Enhancing and Antioxidant Activities of Lycium ruthenicum Murray Anthocyanin Extracts in an Aβ42-Induced Rat Model of Dementia
    WU Xue-ling LI Xiao-xiao JIA Shi-liang GAO Zhao-lan LU Zheng DAI Xue-ling SUN Ya-xuan
    2017, 33(3):29-34. DOI: 10.13982/j.mfst.1673-9078.2017.3.005
    [Abstract](885) [HTML](207) [PDF 869.75 K](996)
    Abstract:
    Amyloid beta-protein 1-42 (Aβ42) was injected bilaterally into the hippocampal CA1 area of rats to produce an animal model of Alzheimer′s disease (AD), and animal behavior was assessed using the passive-avoidance test in a shuttle box. The activities of total superoxide dismutase (T-SOD) and catalase (CAT), and the contents of glutathione (GSH), malondialdehyde (MDA), and protein carbonyl (PC) in serum and brain tissues were used as evaluation indicators to investigate the effect of Lycium ruthenicum Murray anthocyanin extracts on the in vivo antioxidant activity and memory function of AD model rats. The results showed that the memory capacity of the model group was significantly decreased, while the memory impairment of rats administered Lycium ruthenicum Murray anthocyanin extracts was alleviated. Additionally, the activities of T-SOD and CAT, and the content of GSH in serum and brain tissues were significantly enhanced, whereas MDA and PC contents were decreased in the group that received 80 mg/kg Lycium ruthenicum Murray anthocyanin extracts. Thus, Lycium ruthenicum Murray anthocyanin extracts could enhance memory capacity and antioxidant activities of AD model rats, and had a potential preventive effect against AD.
    6  Analysis of the Volatile Components of Lithocarpus polystachyus Rehd. and a Preliminary Study of Their Anticancer Activity
    YAN Ni WANG Zuo GAO Xiong ZHANG Yuan-yuan LIN Xiao-rong LI Bin CHEN Zhong-zheng
    2017, 33(3):35-39. DOI: 10.13982/j.mfst.1673-9078.2017.3.006
    [Abstract](590) [HTML](204) [PDF 528.97 K](813)
    Abstract:
    Lithocarpus polystachyus Rehd. was selected as a starting material to extract volatile components using simultaneous distillation extraction (SDE) method. The composition of volatile components was analyzed by gas chromatography–mass spectrometry (GC-MS). Subsequently, the anticancer activities of the volatile components and major monomeric substances of Lithocarpus polystachyus Rehd. were studied using mouse hepatoma cells (Hepa1c1c7). A cell viability of greater than 50% and quinone reductase (QR) induction activity greater than or equal to 2 were set as the activity indicators. The results showed that a total of over 80 types of major volatile compounds were identified by the GC-MS analysis, accounting for 92.56% of the total volatiles. Ketones, alcohols, and aldehydes were the major chemical types. Among them, geranylacetone, β-ionone, nonanal, and spathulenol were the most abundant volatile components. The measurement of anti-cancer activity indicated that the induced QR activity was amplified when the concentration of volatile components of Lithocarpus polystachyus Rehd. was (27.50±0.10) μg/mL. And the concentrations of linalool, geranylacetone, and nonanal, the three main volatile components that enhanced the induced QR activity, were (54.53±0.10) μg/mL, (283.10±0.10) μg/mL, and (297.77±0.12) μg/mL, respectively.
    7  Porphyran Reduces Cytotoxicity Induced by Two Carcinogens in Allium sativum Root Cells
    ZHANG Zhong-shan WANG Xiao-mei LIU Chong-bin
    2017, 33(3):40-46. DOI: 10.13982/j.mfst.1673-9078.2017.3.007
    [Abstract](771) [HTML](145) [PDF 328.35 K](794)
    Abstract:
    Studies have shown that porphyran extracted from the red algae Porphyra haitanesis has a potential antioxidant activity. The effect of porphyran and its sulfate derivatives on cyclophosphamide (CP)- and mitomycin C (MMC)-induced changes in micronucleus rate, mitotic index, chromosome aberration rate, and lipid peroxidation in Allium sativum root cells was investigated. The results indicated that the polysaccharide significantly inhibited the micronucleus rate and chromosomal aberration rate, and significantly enhanced the mitotic index in a dose dependent manner. The polysaccharide exhibited the highest inhibitory and enhancing effects at 100 mg/L concentration. Additionally, the sulfate derivative exhibited superior resistance towards lipid peroxidation in cells compared to that exhibited by the unmodified polysaccharide. The analysis reveals that the anti-carcinogenic effect of porphyran has a definite relationship with anti-lipid peroxidation activity, and shows that the sulfate group has an indirect effect on anti-carcinogenic activity. The results showed that porphyran has an anti-carcinogenic potential.
    8  Synthesis and Characterization of Luteolin Complexes and Their Inhibition Effects on Xanthine Oxidase
    XIAO Kai-jun ZHONG Lian-jun CAI Sheng ZHU Liang GONG Bin ZHU Zi-pei
    2017, 33(3):47-53. DOI: 10.13982/j.mfst.1673-9078.2017.3.008
    [Abstract](811) [HTML](360) [PDF 713.88 K](890)
    Abstract:
    The coordination effect was used to synthesize luteolin complexes between luteolin and metal ions Zn2+ and Mn2+, and the complexes were examined by ultraviolet UV-visible spectroscopy, infrared spectroscopy, thermogravimetric-differential scanning calorimetry, and elemental analysis techniques. Moreover, the inhibition of xanthine oxidase by the luteolin complex was studied, the inhibitory capacities of luteolin and its complexes were compared, and the type of inhibition of xanthine oxidase was determined. The results indicated that coordination reactions occurred between luteolin and the metal ions Zn2+ and Mn2+ to form stable complexes. The chelation site was located at the 5-OH of ring A and 4-C=O of ring C. Compared to luteolin, its complexes exhibited a better inhibitory effect on xanthine oxidase. When the xanthine substrate concentration was 0.6 mM, the graph of enzyme concentration versus enzyme activity showed that the types of inhibition by luteolin and its complexes were reversible. When the concentration of xanthine oxidase was 10 μg/mL, the double reciprocal Lineweaver-Burk plot showed that the types of inhibition by luteolin and its complexes were competitive.
    9  Screening of a Double Auxotrophic Cordyceps militaris Mutant and Its High Fermentation Yield of Cordycepin
    WANG Liang LIU Wen-jing CAO Zhao-ping TIAN Jing JIN Zhao-xia ZHOU Guang-qi
    2017, 33(3):54-59. DOI: 10.13982/j.mfst.1673-9078.2017.3.009
    [Abstract](797) [HTML](226) [PDF 610.30 K](911)
    Abstract:
    Cordycepin, with a variety of medical and health benefits, is one of the key bioactive compounds in Cordyceps militaris. However, the low fermentation yield restricts its industrial production and applications. To enhance the yield of cordycepin, a xanthine-guanine double auxotrophic mutant, xan-gua--c, was obtained by ultrasound mutagenesis combined with diethyl sulfate treatments, followed by directional screening, and its yield of cordycepin was improved to 1.76 g/L on the 20th day of batch fermentation, about 40% higher than that of the control. The effects of structural analogs of cordycepin on the cordycepin production capacity of the mutant were then investigated. The results showed that the addition of 1.0 g/L adenosine and 1.0 g/L adenine could improve the production of cordycepin (on the 20th day) by 33.5% (to 2.35 g/L) and 103.5% (to 3.59 g/L), respectively, compared with that of the control. Metabolic pathway analysis indicated that there might be two metabolic pathways used to synthesize cordycepin from adenine or adenosine. The two metabolic processes might overlap, and blocking the anabolism of xanthine and guanine might be an effective strategy to promote Cordyceps militaris to accumulate cordycepin.
    10  A Comparative Study on the Prevention of Calcium Precipitation by Two Types of Casein Phosphopeptides
    LIANG Xiao-na KONG Yan-wen ZHANG Ju-ming TAO Dong-bing CONG Min YANG Mei LIU Biao WU Jun-rui YUE Xi-qing
    2017, 33(3):60-66. DOI: 10.13982/j.mfst.1673-9078.2017.3.010
    [Abstract](784) [HTML](249) [PDF 587.26 K](919)
    Abstract:
    Casein phosphopeptides (CPPs) are biologically active peptides derived from milk casein that contain phosphoserine clusters. The ability of two CPP samples with different nitrogen/phosphorus (N/P) molar ratios to inhibit precipitation induced by calcium ions was compared, and the effect of pH, temperature, and CPP concentration on the inhibition, and synergistic effects of CPPs with citric acid and xylitol were assessed. The results show that the CPP concentration was the main influencing factor of CPP effectiveness, with pH having the largest effect on CPP, followed by addition of citric acid and xylitol. Temperature had nearly no effect on CPP activity; citric acid and xylitol had a certain synergistic effect on the two types of CPPs, and the synergistic effects of citric acid with the two types of CPPs were similar; effectiveness increased with increasing concentration. With respect to inhibition of metal ion precipitation, pH, CPP concentration, and xylitol synergism had better effectiveness on CPP II activity, but temperature had no effect on either of the CPPs. Both the types of CPPs had good functional properties, but CPP II, which had a lower N/P molar ratio, was better. This study provided a theoretical basis for research and development of CPP-rich functional and nutritional health foods in China.
    11  Cloning, Expression, Purification, and Activity Assay of Acetyl-CoA Synthetase from Dunaliella tertiolecta
    JIN Hong-hao LIANG Ming-hua JIANG Jian-guo
    2017, 33(3):67-73. DOI: 10.13982/j.mfst.1673-9078.2017.3.011
    [Abstract](941) [HTML](218) [PDF 568.56 K](1041)
    Abstract:
    Acetyl-coenzyme A (CoA) synthetase (ACS) catalyzes the synthesis of acetyl-CoA and is one of the important hubs of fat and acetate metabolism. In this study, reverse-transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) techniques were used to obtain ACS cDNA from Dunaliella tertiolecta (DtACS). The cDNA contained 2,464 base pairs (bp) (full-length). The predicted length of open reading frame (ORF) was 2,184 bp, and 727 amino acids were encoded by the ORF. Sequence alignment showed that DtACS shared high identities with ACS from chlorophyta (Chlamydomonas reinhardtii, 68% identity and Volvox carteri f. nagariensis, 70% identity). pET32a (+) with the thioredoxin tag (Trx-tag) was selected as the prokaryotic expression vector, and DtACS was cloned into pET32a (+) to construct pET-32a-DtACS, which was then transformed into an Escherichia coli strain BL21(DE3). The blank vector pET-32a (+) was also transformed, successfully producing pET-32a-DtACS-BL21(DE3) and control pET-32a-BL21(DE3) recombinant bacteria. The recombinant bacteria were induced at 18 ℃for 12 h with a final IPTG concentration of 0.6 mmol/L, and the molecular weight of the expressed DtACS with Trx-His-tagged fusion protein was about 8.74 ku (6.99 ku+1.75 ku). In addition, the recombinant protein was purified by nickel ion affinity chromatography column, and the specific enzyme activity of the purified protein was 52.87 U/mg.
    12  De Novo Biosynthesis of Pinosylvin Using Engineered Escherichia coli
    LIANG Jing-long GUO Li-qiong LIN Jun-fang CAI Fa-ji LU Ya-qin
    2017, 33(3):74-80. DOI: 10.13982/j.mfst.1673-9078.2017.3.012
    [Abstract](1016) [HTML](220) [PDF 712.96 K](1022)
    Abstract:
    Pinosylvin, a stilbenoid analog of the valuable nutrient resveratrol, exhibits many biological effects, such as cardiovascular disease prevention and anticancer and anti-arthritic activities. Herein, genetic engineering procedures were used to develop a de novo method for pinosylvin synthesis in order to ease the current problem of low pinosylvin supply. A constitutive vector containing the phenylalanine ammonia-lyase, cinnamoyl-coenzyme A ligase, and stilbene synthase genes was transformed into the L-phenylalanine-overproducing host strain Escherichia coli ATCC31884. PCR identification confirmed that a recombinant bacterial strain was obtained. The engineered bacterial cells were grown in shaking culture, and the culture medium was measured by HPLC to confirm that an engineered strain with de novo pinosylvin biosynthetic ability had been successfully created. Testing of culture samples taken at different fermentation times revealed that the rate of pinosylvin production increased more slowly after 12 h of culture, the highest pinosylvin yield of 0.32 mg/L was reached after a 24 h fermentation, and the maximum accumulated amount of cinnamic acid was 52.92 mg/L. These results suggest that the engineered E. coli can employ its own metabolism to synthesize pinosylvin de novo without addition of any other substances. However, the conversion efficiency of cinnamic acid was low, and thus further studies are needed to improve the strain.
    13  Release of Phenolic Acid by two Filamentous Fungi during the Fermentation of Wheat Bran with Ferulic Acid Esterase and Xylanase
    YIN Zhi-na WU Hui LAI Fu-rao LEI Zhuo-gui WU Wen-jia MIN Tian HU Bo-han DU Xiao-yan ZHANG Xiao-yuan
    2017, 33(3):81-87. DOI: 10.13982/j.mfst.1673-9078.2017.3.013
    [Abstract](1019) [HTML](223) [PDF 1.17 M](923)
    Abstract:
    In order to evaluate the degradation abilities of two filamentous fungi on wheat bran and the difference in their abilities to release phenolic acid, the changes in the contents of total phenols and nine phenolic acid components were monitored at different time. Based on the activities of ferulic acid esterase and xylanase, the surface structure of wheat bran was examined via electron microscopy, and Aspergillus niger and Aspergillus clavatus were comprehensively evaluated. After seven days of fermentation with Aspergillus niger, the ferulic acid content reached 416.56 μg/g wheat bran, and the gallic acid content increased from 7.71 μg/g wheat bran to 105.77 μg/g wheat bran. The ferulic acid content in the fermentation by Aspergillus clavatus increased to 200.81 μg/g wheat bran. The malic acid, gallic acid, p-hydroxybenzoic acid, and coumaric acid contents increased to 88.4 μg/g wheat bran, 60.38 μg/g wheat bran, 55.56 μg/g wheat bran, and 52.93 μg/g wheat bran from 14.74 μg/g wheat bran, 7.71 μg/g wheat bran, 8.57 μg/g wheat bran, and 5.62 μg/g wheat bran, respectively. Compared with Aspergillus clavatus, Aspergillus niger exhibited significantly higher abilities to degrade wheat bran and release ferulic acid and gallic acid, but a slightly weaker ability to release malic acid. The electron microscopy results showed that the degradation ability of Aspergillus niger was better than that of Aspergillus clavatus.
    14  Characteristics of Rice Tea Digestion
    XIONG Li-cheng ZHAO Si-ming NIU Meng LIU You-ming HU Zhi-quan
    2017, 33(3):88-92. DOI: 10.13982/j.mfst.1673-9078.2017.3.014
    [Abstract](739) [HTML](198) [PDF 520.09 K](854)
    Abstract:
    Rice tea made from soaked and roasted rice was subjected to pancreatic enzyme (glucoamylase and pepsin-trypsin) treatment in order to simulate the digestion of starch and protein in the human body. The effects of animal and vegetable oils, roasting time, and roasting temperature on the characteristics of rice tea starch and protein digestion were investigated to provide a theoretical basis for optimizing the rice tea roasting process. The digestive characteristics were significantly affected by the roasting process, which inhibited starch digestion and thus could reduce the glycemic index. Addition of oil also had significant inhibitory effects on the digestion of rice tea, which involved reducing the amount of glucose in the digestion products, but the type of oil used had little effect on the inhibition of starch digestibility. A higher roasting temperature (above 200 ℃) or time (above 35 min) could reduce the digestibility of the rice tea protein. However, roasting had little effect on the absolute amino acid contents of rice tea after digestion. Although the lysine and threonine contents were decreased by the roasting process, the contents of essential amino acids remained unchanged when the appropriate roasting process was conducted.
    15  Real-time and Droplet Digital PCR Analyses for Detection of Vibrio cholerae Gene Expression by Four Gene Quantification Methods
    TANG Jing JIA Jun-Tao ZHAO Li-Qing WANG Jing ZHANG Jian JIANG Ying-Hui XU Biao WANG Chang-Jun MA Yun
    2017, 33(3):93-98. DOI: 10.13982/j.mfst.1673-9078.2017.3.015
    [Abstract](929) [HTML](477) [PDF 481.76 K](1210)
    Abstract:
    Gene expression assays are highly important for analyzing gene function and investigating biological mechanisms. Currently, real-time and droplet digital PCR are effective methods for measuring gene expression. sfs, vcc, RecA, hly, and 16S rRNA genes of Vibrio cholerae were used for experiments after low temperature treatment. The samples were assessed by real-time and droplet digital PCR separately before and after low temperature treatment. Real-time fluorescence PCR experimental data were analyzed using the ??CT and geNorm methods. Droplet digital PCR experimental data were analyzed using the relative and absolute quantification methods to calculate the fold change in gene expression in the treatment group as compared to the control group. The data from the four gene quantification methods were analyzed using the multidimensional scaling method. Under these experimental conditions, the results indicated that there was a significant difference between the results of the four gene quantification methods. The expression level of the 16S rRNA gene changed, making it unsuitable as a reference gene. Droplet digital PCR could give more intuitive results regarding the amount of change in gene expression and have better accuracy in the relative quantitative analysis of differentially expressed genes.
    16  Properties of Cellulose Sulfate Aqueous Solution
    RUAN Zhi-yang WANG Zhao-mei GU Yi-shan XIAO Kai-jun
    2017, 33(3):99-104. DOI: 10.13982/j.mfst.1673-9078.2017.3.016
    [Abstract](840) [HTML](170) [PDF 549.38 K](884)
    Abstract:
    Cellulose sulfates (CS) were prepared by heterogeneous sulfation esterification of α-cellulose, using chlorosulfonic acid in dimethylformamide as the sulfation agent. The degree of substitution (DS) of sulfate groups in CS was approximately 2.0, as determined by elemental analysis. Carbon-13 nuclear magnetic resonance (13C-NMR) spectroscopy data suggested that the sulfation pattern of CS included complete C-6 substitution as well as complete or partial C-2 substitution. CS showed good solubility in deionized water. Upon using a series of CS solutions with similar DS and fitting with Mark-Houwink equation, the sugar chains of CS showed a relatively compact conformation in dilute solution, based on the relationship between molecular weight and intrinsic viscosity. At high concentration, the dynamic viscosity of CS increased with an increase in CS concentration. The addition of salt promoted charge repulsion within the polyelectrolyte in CS, resulting in an increase in dynamic viscosity of CS. The addition of Ca2+ triggered complexation between CS and Ca2+ and significantly enhanced the dynamic viscosity of CS. The pH value of the solution was one of the main factors influencing the dynamic viscosity of CS, which was highest at neutral pH and decreased at acidic or alkaline pH values.
    17  Expression of the Human Lysozyme Gene in Kluyveromyces lactis
    LIU Si-tong XU Long-long YI Ze-hao LIU Xiao-jing HUI Feng-li
    2017, 33(3):105-109. DOI: 10.13982/j.mfst.1673-9078.2017.3.017
    [Abstract](975) [HTML](176) [PDF 646.89 K](1071)
    Abstract:
    Human lysozyme, an enzyme with natural broad-spectrum antimicrobial activity, has potential applications in the food and pharmaceutical industries. To obtain human lysozyme preparations with high activity, a Kluyveromyces lactis yeast expression system was used for soluble expression of the human lysozyme gene (hLYZ) with optimized codons. An artificial hLYZ gene was cloned into the K. lactis expression vector pKLAC1, resulting in the recombinant vector pKLAC1-hLZY, which was then linearized with SacII and transformed into K. lactis GG799 by electroporation. Following whole-cell PCR, a multicopy recombinant strain (named hLYZ1) was obtained. The engineered strain secreted a target protein of approximately 14 ku in molecular weight, which agreed with that of the expected product. The maximum activity of hLZY in the culture supernatant reached 1430 U/mL after 128 h of culture. Antibacterial activity testing confirmed that the recombinant human lysozyme was effective against Micrococcus luteus, Escherichia coli, and Bacillus subtilis. In conclusion, a recombinant hLYZ with high enzyme activity was successfully expressed in K. lactis GG799, thereby providing a basis for studies on the large-scale production of recombinant human lysozyme using the K. lactis expression system.
    18  Establishment of a Heat Transfer Model of Silver Carp Muscle during Freezing and Experimental Verification
    LIU Qing XIONG Shan-bai LU Chang-xin HU Yang LIU Ru
    2017, 33(3):110-115. DOI: 10.13982/j.mfst.1673-9078.2017.3.018
    [Abstract](804) [HTML](232) [PDF 535.97 K](963)
    Abstract:
    Accurate simulation of the freezing process and prediction of freezing time are important for the design and control of freezing devices and assurance of product quality. Based on the prediction model of thermophysical parameters and an enthalpy-based mathematical model for silver carp muscle, a finite difference method was adopted to establish a physical model for flat silver carp muscle. The freezing process and freezing time for the thermal center and boundary layer of the silver carp muscle were also predicted. The results showed that the mean sums of squared differences between the simulated value and experimental data of the thermal center and boundary layer of the silver carp muscle with a thickness of 44 mm were 1.85 and 1.30, respectively. The relative error of the predicted freezing time versus real-time was -1.64%. Based on these results, the cooling processes for the thermal center of silver carp muscle with thicknesses of 24 mm and 34 mm were simulated, and the corresponding mean sums of squared differences between the simulated value and experimental data of the thermal center were 1.73 and 1.58, respectively. In conclusion, the model established using a combination of the prediction model of thermophysical parameters and numerical simulation showed high simulation accuracy for the freezing process of silver carp muscle with different thicknesses, providing a new simulation method for the freezing, processing, and quality control of freshwater fish and equipment design.
    19  Effects of Galactose on the Reaction Kinetics of Thermal Degradation of TMAO in Squid
    CHEN Shua ZHU Jun-li PAN Wei-chun
    2017, 33(3):116-121. DOI: 10.13982/j.mfst.1673-9078.2017.3.019
    [Abstract](803) [HTML](199) [PDF 638.08 K](879)
    Abstract:
    The aim of this study was to assess the endogenous stimulating factors of thermal decomposition of trimethylamine-N-oxide (TMAO). The influence of reducing sugar on the thermal conversion of TMAO to formaldehyde (FA), dimethylamine (DMA), and trimethylamine (TMA) in squid was studied using thermal decomposition kinetics. At high temperatures, the TMAO in squid was decomposed into FA, DMA, and TMA, and the content of the reducing sugar was reduced. In vitro screening demonstrated that the additions of seven types of reducing sugars could significantly promote FA production in the supernatant of squid and the TMAO-Fe(II) system. Among them, galactose exhibited the strongest stimulation effect, and a similar promoting effect was observed in the supernatant of squid and the TMAO-Fe(II) system on the decomposition of TMAO into FA, DMA, and TMA. Reaction kinetics of the thermal decomposition of TMAO in TMAO-Fe(II) and TMAO-Fe(II)-Gal were studied comparatively. The changes in the contents of FA, DMA, TMA, and TMAO followed the zero order kinetics. The thermal decomposition of TMAO was divided into two stages, in which the apparent activation energies of stage 2 were lower than those of stage 1. The addition of galactose reduced the activation energies significantly. Therefore, the addition of galactose and other reducing sugars should be limited during the processing of squid products to control the decomposition of TMAO to FA.
    20  Storage Characteristics and Shelf-life Prediction of Spicy Beef under Different Storage Temperatures
    DING Jie HU Xin-jie LU Xue-song HE Jiang-hong LU Yi QIN Wen FU Ting-ting ZHU Jin-yan YI Yu-wen
    2017, 33(3):122-132. DOI: 10.13982/j.mfst.1673-9078.2017.3.020
    [Abstract](904) [HTML](236) [PDF 628.24 K](952)
    Abstract:
    Using different temperatures for storage of spicy beef, quality indicators and Pearson’s correlation coefficients of quality parameters during storage were evaluated, and a shelf-life prediction model was established. The results revealed that during storage, pH increased after an initial decrease, the total bacterial count, the total volatile base nitrogen (TVB-N) value, and thiobarbituric acid (TBA) value increased, whereas the sensory quality and hardness of spicy beef decreased. Lower storage temperature resulted in better product quality and longer shelf life. The shelf-life values of spicy beef stored at 5 ℃, 15 ℃, 25 ℃, 37 ℃, and 45 ℃ were 16.0, 12.5, 8.5, 3.0, and 1.5 days, respectively. The total bacterial count showed a significant correlation with sensory quality, pH, TVB-N, TBA, and hardness (p<0.01). Multiple regression model, the regression model for the correlation between temperature and shelf life, and kinetic shelf life models (tTVB-N, tTVB-N, and tHardness) were developed based on dynamic changes in product characteristics during storage. Validation of the models revealed that the regression model for the correlation between temperature and shelf life rapidly and accurately predicted the shelf life of spicy beef in a temperature range of 5 ℃~25 ℃, with relative error ranging between 0.29% and 2.36%. Good prediction results were achieved using the multiple regression models within the temperature range of 5 ℃ to 37 ℃, with relative error ranging between 1.07% and 7.89%. The kinetic shelf-life models (tTVB-N, tTVB-N, and tHardness) exhibited poor prediction results.
    21  A Study on the Gelling Properties of Plaster Tofu Gel
    SUN Xiao-lu GU Zhen-yu YANG Yue-xi
    2017, 33(3):133-138. DOI: 10.13982/j.mfst.1673-9078.2017.3.021
    [Abstract](849) [HTML](314) [PDF 1.04 M](1225)
    Abstract:
    The coagulation process is an extremely crucial step during preparation of plaster tofu as the quality and yield of tofu are influenced by the gelling properties during this process. The gelling properties of plaster tofu were characterized by dynamic rheological testing, texture analysis, and scanning electron microscopy, considering changes in coagulation temperature and calcium sulfate concentration. Dynamic viscoelastic testing showed that the highest storage modulus of tofu gel is obtained at 85 ℃ regardless of the calcium sulfate concentration, and the relationship between storage modulus and coagulant temperature followed first-order reaction kinetics. Texture analysis revealed that the hardness of tofu increased with increasing coagulation temperature at a calcium sulfate concentration of 20~50 mM. At the calcium sulfate concentration of 20 mM or 30 mM, the tofu gel was fragile with a loose gel network, which gradually became thicker and more elastic with increased pore size as the coagulation temperature increased, and the highest water holding capacity was reached at 85 ℃. At the calcium sulfate concentration of 40 mM or 50 mM, elasticity of the tofu gel decreased with an increase in coagulation temperature; the tofu gel was thick and dense, and larger pore size and irregular morphology were observed with an increase in coagulation temperature. The highest water holding capacity of tofu gel was obtained at 80 ℃ and 75 ℃.
    22  Preparation of Zein Microparticles Loaded with Astaxanthin by a Supercritical Carbon Dioxide Anti-solvent Process
    HU Man JIANG Yan-bin JIAN Hua-li SONG Guang-jun
    2017, 33(3):139-145. DOI: 10.13982/j.mfst.1673-9078.2017.3.022
    [Abstract](901) [HTML](218) [PDF 777.05 K](1083)
    Abstract:
    Astaxanthin-loaded microparticles were created using a supercritical anti-solvent (SAS) process in this study, with zein as the carrier material, a mixture of dichloromethane and dimethyl sulfoxide as co-solvent, and supercritical carbon dioxide as the anti-solvent. The effects of five operating conditions, i.e., solvent ratio, temperature, pressure, concentration of carrier materials, and solution flow rate, on astaxanthin encapsulation rate, particle morphology, and mass median diameter (Dp50) were investigated using an OA25 (55) orthogonal experimental design. Analysis of variance indicated that the optimal operating conditions were as follows: DMSO/DCM (1:2, V/V), T = 42 ℃, P=80 bar, C=3 g/L, and F=1.5 mL/min. Under these conditions, the astaxanthin encapsulation rate was 94.4% and Dp50 was 385.4 nm. Scanning electron microscopy (SEM) images showed that most of the particles had a spherical shape with a smooth surface, and Fourier transform infrared (FT-IR) spectroscopy suggested that the chemical structure of astaxanthin was not changed after the SAS process. X-ray powder diffraction (XRD) patterns indicated that astaxanthin was embedded completely in zein, and the protective effect of the carrier material on astaxanthin was achieved. The results of storage tests indicated that the stability of astaxanthin was improved significantly after loading.
    23  Analysis of the Rheological Properties of Raw Dehydrated Potato Flour by Mixolab
    ZENG Fan-kui LIU Gang LIN Gang YU Hui GAO Guo-qiang WEN Guo-hong
    2017, 33(3):146-154. DOI: 10.13982/j.mfst.1673-9078.2017.3.023
    [Abstract](830) [HTML](177) [PDF 670.31 K](960)
    Abstract:
    The rheological properties of raw dehydrated potato flour were evaluated by analyzing the thermo-mechanical properties using Mixolab. Eleven raw dehydrated potato flours were used as the experimental material, and the repeatability of using Mixolab to determine the rheological properties of raw dehydrated potato flour was explored. The results indicated that the test curves of each sample exhibited a high degree of overlap (n=2). The average variation coefficient of total curve was less than 5%. There were significant differences in the rheological properties of different potato cultivars, as determined by comparing the test results of samples B01~B10. These differences were found on the indicators at the stages of dough formation, dough stability, starch gelatinization, and retrogradation. The B06 and B10 samples that were prepared by different grinding methods were compared, and the results revealed that the grinding process used for the preparation of raw dehydrated potato flour could significantly affect the water absorption and viscosity values of samples, but have only slight impact on the indices of weakening consistency (Cs and C2). Comparison of the thermo-mechanical properties of raw dehydrated potato flour, potato flakes, and standard wheat flour indicated that potato flakes mainly played a role as a filling agent in the development and processing of potato staple foods. Raw dehydrated potato flour has excellent processability, and its rheological properties can also be further improved by adding food additives.So that it is possible to produce pure potato staple foods (steamed bread, noodles and bread) without adding wheat flour.
    24  Impact of Vibration Stress on the Cell Wall Metabolism of Apricot Fruits
    WU Fang CHENG Xi WANG Ying ZHU Xuan
    2017, 33(3):155-161. DOI: 10.13982/j.mfst.1673-9078.2017.3.024
    [Abstract](799) [HTML](204) [PDF 730.81 K](914)
    Abstract:
    Xinjiang “Saimaiti” apricot was the experimental material analyzed in this study. At a temperature of 4 ℃ and a relative humidity of 90%~95%, the apricot fruits with and without foam-net were placed on a homemade vibration table with a controllable amplitude and variable frequency. The vibration treatment was conducted horizontally and vertically at a simultaneous vibration frequency of 5 Hz to simulate vehicle vibration during transportation. The apricot fruits were vibrated for 12 h, 24 h, and 48 h, and stored at 4 ℃ and 90 %~95 % relative humidity. Fruits with no vibration were used as controls. Sampling was carried out every seven days, and fruit firmness, proto-pectin content, water-soluble pectin content, cellulose content, and polygalacturonase (PG), cellulase (Cx), and β-glucosidase activities were measured and the effect of vibration stress on the cell wall metabolism of the fruits was studied. The results showed that vibration stress could increase the water-soluble pectin content, cellulose content, as well as PG, Cx, and β-glucosidase activities of apricots. This also decreases the firmness and proto-pectin content of apricots, as well as accelerates the quality deterioration and softening process of apricots. Compared with no foam-net packaging, foam-net could inhibit the above physiological processes and increase the hardness of apricot fruits. This indicates that the cell wall metabolism and degradation of apricots with foam-net packaging were reduced more effectively than those without foam-net packaging during storage.
    25  Effect of Ultrasound-induced Maillard Reaction on Modification of Protein Hydrolysate fromYellow Stripe Trevally (Selaroides leptolepis) Fish Meat and Potential Hazards
    WANG Zhong-he WANG Jun FU Li LU Bin
    2017, 33(3):162-170. DOI: DOI: 10.13982/j.mfst.1673-9078.2017.3.025
    [Abstract](851) [HTML](257) [PDF 734.42 K](952)
    Abstract:
    Ultrasound was used to promote the Maillard reaction between protein hydrolysates of Selaroides leptolepis and glucose. The effect of the Maillard reaction on the functional properties and antioxidant activities of hydrolysates as well as formation of potentially harmful compounds were evaluated. The results showed that free amino group content decreased while grafting degree correspondingly increased with increasing ultrasound power or reaction time. Moreover, ultrasound treatment promoted the Maillard reaction and decreased the formation of potentially harmful compounds, such as acrylamide. Analysis of functional properties indicated that the ultrasound-induced Maillard reaction significantly enhanced the solubility and emulsifying capacity of hydrolysates (p<0.05). Additionally, in vitro digestion experiments showed that ultrasound treatment reduced the digestibility of hydrolysates. The 2,2-diphenyl-1-picrylhydrazyl scavenging ability, reducing power, and Fe2+-chelating ability of the modified products were significantly increased (p<0.05), and the modification capability of ultrasound and properties of modified products were better than those of the conventional water bath group. These results showed that the ultrasound-induced Maillard reaction could enhance the functional properties and antioxidant activity of protein hydrolysates of yellow stripe trevally and decrease the formation of acrylamide and other potentially harmful compounds.
    26  Effects of Composite Plasticizer Polyethylene Glycol 200 and Glycerol on the Properties of Starch/PHA Composite Films
    SUN Sheng-lin LIU Peng-fei WANG Wen-tao HOU Han-xue DONG Hai-zhou
    2017, 33(3):171-178. DOI: 10.13982/j.mfst.1673-9078.2017.3.026
    [Abstract](1062) [HTML](233) [PDF 784.12 K](1335)
    Abstract:
    Starch/polyhydroxyalkanoate (PHA) composite films were prepared using the blown film extrusion method with hydroxypropylated crosslinked tapioca starch and PHA as the film-forming substrate, glycerol (GLY) as the primary plasticizer, polyethylene glycol 200 (PEG200) as the secondary plasticizer, and organically modified montmorillonite (OMMT) as the enhancer. The effect of weight ratios of PEG200/GLY on the properties of the composite films was evaluated. The structure, microstructure, and molecular interactions of the films were analyzed by X-ray diffraction, scanning electron microscope, and Fourier transform infrared spectroscopy, respectively. The water vapor barrier properties, mechanical properties, light transmittance, and color of the films were also measured. The results showed that the primary plasticizer GLY had a good plasticizing effect on the starch/PHA blend systems. Moreover, the addition of PEG200 facilitated the formation of intercalated nanostructures between starch, PHA, and OMMT, and increased the light transmittance and whiteness values of the composite films. Additionally, the surface of the composite films became flat and smooth. When the weight ratio of PEG200/GLY was 8/92, the composite films showed good moisture barrier properties and high elongation at break. When the weight ratio of PEG200/GLY was 12/88, the composite films showed a high tensile strength and good light transmittance. The addition of the secondary plasticizer PEG200 enhanced intermolecular interactions. During the preparation of starch/PHA composite films, PEG200 was a preferable compatibilizer and plasticizer, and the weight ratio of PEG200/GLY should be less than 12/88.
    27  Effect of Surfactants on T1 Lipase Activity
    TANG Qing-yun WANG Yong-hua
    2017, 33(3):179-183. DOI: 10.13982/j.mfst.1673-9078.2017.3.027
    [Abstract](942) [HTML](307) [PDF 535.74 K](1169)
    Abstract:
    T1 lipase was incubated with nonionic, cationic (CTAB), anionic (N-L and sodium dodecyl sulphate [SDS]), and zwitterionic (SB3-14) surfactants at different concentrations, and subsequent changes in enzymatic activity was measured using p-nitrophenyl dodecanoate as substrate. The results showed that nonionic surfactants at low concentrations activated T1 lipase, whose relative activity increased by 50% to 150%. Conversely, T1 lipase activity was inhibited to various degrees when the concentration of nonionic surfactants exceeded their critical micelle concentration, and the most significant inhibitory effect was observed with Tween 80. The cationic, anionic, and zwitterionic surfactants strongly inhibited T1 lipase activity, probably due to strong charge repulsions produced between the charged groups of the surfactant and the charged amino acids on the protein surface, which may have led to protein denaturation and inactivation. On the other hand, SDS and SB3-14 at low concentrations slightly activated T1 lipase. Since T1 lipase is a thermostable lipase with high industrial application value, it would be useful to further investigate the effects of different surfactants on T1 lipase activity to help select suitable surfactants for different purposes.
    28  Dynamic High-pressure Microfluidization-assisted Method for Extraction of Total Flavonoids from Cyperus esculentus L. Leaves and Application in Cookies
    GUO Gai JIAN Tian-tian QI Rui QU Lei MA Xue-ming REDILI?ABULA JING Si-qun
    2017, 33(3):184-190. DOI: 10.13982/j.mfst.1673-9078.2017.3.028
    [Abstract](1031) [HTML](268) [PDF 673.62 K](867)
    Abstract:
    The optimum conditions for extraction of flavonoids from Cyperus esculentus L. (CE L.) leaves by dynamic high-pressure microfluidization-assisted method (DHPM) was determined through single factor and orthogonal experiments. The yield of flavonoids and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging rate were used as indices. The effect of DHPM on the structure and composition of total flavonoids from CE L. leaves was preliminarily analyzed by ultraviolet-visible and infrared spectral analyses and liquid chromatography–mass spectrometry (LC-MS). At the same time, peroxide value (POV) and acid value (AV) were used as indicators to investigate the effect of total flavonoids from CE L. leaves on the storability of cookies. The results showed the following conditions of DHPM as optimum: microfluidization pressure=120 MPa; extraction temperature=80 ℃; ethanol concentration=80%; extraction time=90 min. Under these optimal extraction conditions, the yield of flavonoids was 1.46% and the half-maximal inhibitory concentration (IC50) value for DPPH scavenging activity was 0.17±0.02 mg/mL. The DHPM-assisted extraction enhanced the yield and antioxidant activities of total flavonoids from CE L. leaves. The results of LC-MS analysis indicated that the treatment of DHPM did not change the composition of total flavonoids from CE L. leaves; however, the ratio of some constituents were altered. The results of POV and AV showed that total flavonoids from CE L. leaves could serve as natural antioxidants for cookie production.
    29  Preparation of Ciprofloxacin Derivative and its Antibody Specificity
    ZHANG Ming-zhu FENG Ting-ting WEI Dong
    2017, 33(3):191-195. DOI: 10.13982/j.mfst.1673-9078.2017.3.029
    [Abstract](816) [HTML](209) [PDF 544.33 K](798)
    Abstract:
    The preparations of the ciprofloxacin (CPLX) carboxyl derivative and its antibody were studied in this paper, and the antibody specificity of this derivative was determined. CPLX derivatives (CPLX-COOH) were synthesized with CPLX and 3-bromopropionic acid, and purified by preparation of liquid phase. Infrared spectroscopy (IR), electrospray ionization mass spectrometry (ESI-MS), and nuclear magnetic resonance (NMR) spectroscopy were used to identify the CPLX derivative. CPLX-COOH was conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) to prepare the immune antigen CPLX-COOH-BSA and coating antigen CPLX-COOH-OVA, which were identified using ultraviolet spectroscopy. The BALB/c mice were immunized with CPLX-COOH-BSA to obtain a polyclonal antibody directed against the CPLX derivative with high titer. The optimum concentration of the coating antigen and the optimum dilution of the antibody were determined using the chessboard method, and the indirect competitive enzyme-linked immunosorbent assay (ELISA) was established. The half-maximal inhibitory concentration (IC50) and the limit of detection (LOD) of CPLX-COOH were 228.56 ng/mL and 25.527 ng/mL, respectively. This antibody had a broad specificity and cross reactivity with multiple fluoroquinolones, and could be used to detect several fluoroquinolone residues.
    30  Effect of Compound Essential Oil Microcapsule on the Preservation of Rice by Accelerated Shelf Life Testing
    DENG Jing LI Wen LIN Qin-lu ZHANG Jing XIONG Ting LIU Mei-jun
    2017, 33(3):196-202. DOI: 10.13982/j.mfst.1673-9078.2017.3.030
    [Abstract](774) [HTML](176) [PDF 755.56 K](936)
    Abstract:
    The microcapsule preservative was prepared using the co-precipitation method with β-cyclodextrin as the wall material and the compound essential oil (garlic oil/cinnamon oil), which inhibits rice pathogen Aspergillus flavus, as the filling material. The microcapsule preservative was packed in small non-woven bags and put into polyethylene (PE) bags containing rice. The fresh-keeping effect of microcapsules on rice was evaluated through the change in the fatty acid value of rice by accelerated shelf life testing (ASLT), and the shelf life of rice was predicted. Additionally, the correlation between sensory evaluation and the fatty acid value of rice was analyzed, providing a reference for a rapid determination of rice quality. The results showed that garlic oil and cinnamon oil have a strong antifungal effect on Aspergillus flavus, and the highest antifungal effect of the compound essential oil was achieved at a garlic oil to cinnamon oil ratio of 1:3. Scanning electron microscopy (SEM) characterization analysis showed that the microcapsules were irregularly shaped, and the rate of change in the fatty acid value of rice with the compound essential oil microcapsule was slower than that of the control group. The predicted shelf life of rice with the compound essential oil microcapsule was 415 d at 25 ℃, and that of the control group was only 122 d. There was a correlation between sensory evaluation and the fatty acid value of rice.
    31  Effects of Hydrolysate Concentrations and Oil Volume Fractions on the Emulsifying Properties of Sweet Potato Protein Hydrolysates
    CUI Shan-shan MU Tai-hua ZHANG Miao SUN Hong-nan CHEN Jing-wang
    2017, 33(3):203-209. DOI: 10.13982/j.mfst.1673-9078.2017.3.031
    [Abstract](724) [HTML](194) [PDF 586.29 K](1088)
    Abstract:
    The effects of hydrolysate concentrations (0.10%, 0.25%, 0.50%, 0.75%, and 1.00%, m/V) and oil volume fractions (5%, 15%, 25%, 35% and 45%, V/V) on the microstructure, average particle size (d4,3), emulsifying activity index (EAI), emulsion stability index (ESI), and rheological properties of the emulsion of sweet potato protein hydrolysates prepared under high pressure were evaluated. When the hydrolysate concentration was low and the oil volume fraction was high, the particles of emulsion were uniform and small. An increase in the protein concentration resulted in a gradual decrease in the d4,3, EAI and ESI of the emulsions. However, an increase in the oil volume fraction led to a gradual reduction of d4,3, and a gradual increase in the EAI of the emulsion. ESI initially increased but was then reduced (p<0.05). When the hydrolysate concentration was low and the oil volume fraction was high, the initial apparent viscosity of the emulsion was also high, exhibiting the phenomenon of shear thinning within the shear rate range of 1~100 s-1. The hydrolysate concentration and the oil volume fraction were closely related to the d4,3, EAI, ESI, and rheological properties of the emulsion. These were the important factors affecting the emulsifying property of sweet potato protein hydrolysates.
    32  Effect of Blanching Treatment on the Volatile Components of Lentinus edodes
    LIU Lu HUANG Wen CHENG Wei GAO Hong SHI De-fang QIAO Yu
    2017, 33(3):210-215. DOI: 10.13982/j.mfst.1673-9078.2017.3.032
    [Abstract](805) [HTML](236) [PDF 475.93 K](900)
    Abstract:
    The effects of microwave, steam, and boiling-water blanching treatments on the volatile components of Lentinus edodes were compared. Headspace solid-phase microextraction-gas chromatography-mass spectrometry (GC-MS) was employed to qualitatively analyze the volatile components. The external standard method was employed for the quantitative analysis of benzaldehyde and 1-octen-3-ol. Five different types, totaling 25 volatile components, were obtained from Lentinus edodes, including six alcohols, four aldehydes, two ketones, five hydrocarbons, and eight sulfur-containing compounds. The contents of benzaldehyde and 1-octen-3-ol in fresh Lentinus edodes were 0.28 and 308.44 ng/g, respectively. The content of 1-octen-3-ol of Lentinus edodes upon microwave blanching treatment was 515.65 ng/g. The contents of alcohols and sulfur-containing compounds were significantly affected by steam and boiling-water blanching treatments, where steam treatment showed a reduction from 22.49% to 3.93% in the relative content of alcohols, and an increase from 64.57% to 87.64% in the relative amount of sulfur-containing compounds. The contents of benzaldehyde and 1-octen-3-ol after steam treatment were 0.45 and 245.64 ng/g, respectively. Blanching by boiling water resulted in relative content of alcohols at 87.89%. Additionally, the content of sulfur-containing compounds was significantly reduced (2.85%) and the content of 1-octen-3-ol was 1482.46 ng/g. These results suggested that steam and boiling-water blanching processes had a significant impact on volatile components, where contents of alcohols and sulfur compounds were especially significantly different.
    33  Effects of Moisture on the Storage Stability of Lactobacillus casei LC-134 Lyophilized Powder
    BAO Zhi-ning WEI Pei-pei LIN Wei-feng
    2017, 33(3):216-221. DOI: 10.13982/j.mfst.1673-9078.2017.3.033
    [Abstract](776) [HTML](323) [PDF 537.15 K](823)
    Abstract:
    The effects of moisture content and water activity (aw) on the storage stability of Lactobacillus casei LC-134 (LC-134) lyophilized powder were studied. LC-134 lyophilized powders with different moisture contents and aw values were stored at -18 ℃ for 12 months and at 25 ℃ for six months, respectively. Viable cell count variation of the powders were measured regularly, and the influence of polydextrose concentration in the lyoprotectant on the moisture content and aw of the powders was explored. The results showed that during 12 months of storage at -18 ℃, no significant decrease was found in the viable cell counts of the LC-134 lyophilized powders with a moisture content of less than (5.14±0.06)?10-2 g/g or an aw value of less than 0.31±0.01. However, the viable cell count was stable at 25 ℃ during six months of storage if the moisture content was less than (3.06±0.05)?10-2 g/g or aw was less than 0.25±0.01. The addition of polydextrose could reduce the aw value of LC-134 lyophilized powders with the same moisture content. Moreover, the higher the moisture content, the more significant the effect.
    34  Effects of Different Processing Methods on the Physicochemical Properties of Defatted Rice Bran as Food Ingredients
    LIU Lei RAN Yu-bing HAN Su-yun ZHANG Ming-wei ZHANG Rui-fen WEI Zhen-cheng MA Yong-xuan
    2017, 33(3):222-228. DOI: 10.13982/j.mfst.1673-9078.2017.3.034
    [Abstract](813) [HTML](177) [PDF 510.41 K](932)
    Abstract:
    In order to improve the physicochemical properties of rice bran and develop new rice bran-based food ingredients, the effects of three kinds of processing methods, extrusion, microwave cooking, and high temperature roasting, on the physicochemical properties of defatted rice bran were investigated. The results showed that after the defatted rice bran was processed by the three integrated processing methods, the physicochemical properties of samples were improved. Among them, the rice bran with extrusion treatment demonstrated the highest water solubility index (WSI), water absorption index (WAI), degree of gelatinization, and dispersion stability. Compared to those of the control group, the WSI, WAI, and gelatinization degree of extruded rice bran were increased by 4.82%, 18.92%, and 96.04% respectively. However, the three kinds of integrated processing methods reduced the reducing sugar and phytic acid contents of rice bran significantly. This investigation of phenolics and antioxidant capacity showed that high temperature roasting treatment increased the total phenolic content and antioxidant capacity of defatted rice bran significantly, but extrusion treatment reduced the total phenolic content and antioxidant capacity. This study provides guidance for the processing of defatted rice bran as ingredients in powder nutritional meal replacements.
    35  Effect of Photons on the Preservation of Steamed Bread
    LIU Xiu-li LI Zhen-jing ZHOU Qing-li
    2017, 33(3):229-236. DOI: 10.13982/j.mfst.1673-9078.2017.3.035
    [Abstract](814) [HTML](242) [PDF 853.10 K](946)
    Abstract:
    To investigate the effect of photons on the preservation of steamed bread, fresh steamed bread was used as the raw material and sensory evaluation was employed. The effects of photon fields on the texture, viscosity characteristics, starch retrogradation, and prediction of protein secondary structure of steamed bread were studied using a TA-XT.Plus texture analyzer, scanning electron microscope (SEM), rapid viscosity analyzer (RVA), differential scanning calorimeter (DSC), and Fourier transform infrared (FT-IR) spectroscopy. The results indicated that, after the samples were stored at 4 ℃ for 2 d, 4 d, 7 d, and 12 d, the sensory scores of the photon-treated group were all higher than those of control group and mold spots appeared on the control samples on day 12. With increasing storage time, the water loss rate, hardness, and retrogradation degree of steamed bread in the photon-treated group were lower than those of the control group. Moreover, the amounts of β-sheet and β-turns of the photon-treated group were higher and lower than those of the control group, respectively. The above results revealed that the preservation effects of photons on steamed bread could be achieved by affecting the starch retrogradation and protein secondary structure of steamed bread.
    36  Effects of Hot-air Treatment on the Senescence and Softening of Fresh Jujube Fruits during Cold Storage
    LI Jun-lan MA Yong KANG Hui-ren FENG Jiu-hai LIU Zhi-fang
    2017, 33(3):237-243. DOI: 10.13982/j.mfst.1673-9078.2017.3.036
    [Abstract](827) [HTML](236) [PDF 586.81 K](788)
    Abstract:
    Decay, softening, water loss, and wilting can easily occur during the storage of fresh jujube fruits, resulting in an extremely short storage duration for fresh fruits. In order to investigate the influence of hot-air treatment on the postharvest senescence and softening of fresh jujube and related enzyme activities, ‘Linze Xiaozao’ jujube fruit was used as a test material. It was pre-treated with hot air at 45 ℃ for two hours and then packed into folded polyethylene bags (thickness: 0.01 mm) and stored at (0 ℃±0.5). The respiration intensity, relative conductivity, contents of malondialdehyde (MDA), vitamin C, titratable acid (TA), activities of catalase (CAT), ascorbic acid peroxidase (APX), lipoxygenase (LOX), and superoxide dismutase (SOD), among other indicators of fresh jujube fruits were determined every ten days. The results showed that Linze Xiaozao was a climacteric fruit in a respiration pattern. Compared with CK, the hot-air treatment at 45 ℃ for 2 h could effectively inhibit respiration intensity (p<0.05), decrease the activities of LOX and APX, and maintain relatively high contents of vitamin C and TA. The relative conductivity and the MDA content of the pulp tissues significantly decreased (p<0.05). However, the rate of the decline of CAT activity of fresh jujube slowed down and the appearance of SOD activity peak was delayed. Besides, the activity of the enzyme was maintained. In conclusion, hot-air treatment at 45 ℃ for two hours could significantly delay the senescence and softening of postharvest jujube fruits and maintain the storage quality.
    37  A Study on the Rate of Change in the Quality of Different Brown Rice Varieties during Storage
    GAO Shu-cheng ZHAO Xu LIN Zi-mu
    2017, 33(3):244-250. DOI: 10.13982/j.mfst.1673-9078.2017.3.037
    [Abstract](663) [HTML](196) [PDF 675.74 K](931)
    Abstract:
    To investigate the rate of change and factors influencing the quality of brown rice during storage, several rice varieties common in the Liaoning area were selected as experimental materials-Liaoxing, Yanjing 218, Tianfeng 202, Jindao 201, and Yanfeng 407. The moisture content, fatty acid value, conductivity, catalase activity, germination rate, taste evaluation value, amylose content, and other indicators of quality of brown rice samples were assessed and measured at quasi-low temperature (20?℃) and conventional agricultural storage for 1 year (2014.12~2015.12). Furthermore, correlation analysis of the measured data was performed. The results showed that with increased storage time, fatty acid value, and conductivity gradually increased, whereas moisture, catalase activity, germination rate, and taste evaluation value gradually decreased. Differences in storage methods and rice varieties also had significant effects on indicators of the quality of stored brown rice (p<0.05). Comprehensive analysis of the changes in brown rice quality indicators revealed that Tianfeng 202 and Jindao 201 are more suitable for storage. In addition, correlation analysis of the storage quality index of Tianfeng 202 was performed, and results showed that catalase activity, conductivity, fatty acid value, and germination rate can be used as indicators reflecting the storage stability of brown rice.
    38  Impact of Tannin on the Properties of Gelatin Edible Film
    WANG Kun WANG Wen-hang ZHANG Yi LIU An-jun
    2017, 33(3):251-256. DOI: 10.13982/j.mfst.1673-9078.2017.3.038
    [Abstract](1117) [HTML](238) [PDF 627.66 K](1277)
    Abstract:
    Methods to improve the mechanical and barrier properties of gelatin film have attracted considerable attention in the field of edible film manufacture. Here, different concentrations of tannins (0, 10, 20, 30, and 40 mg/g) were added to the gelatin solution and edible film was prepared by the casting method. The physicochemical properties of the film were investigated. The results suggested that incorporation of tannin effectively increased the tensile strength (TS), but reduced the elongation at break (EAB) of the film. The highest TS (22.10 MPa) and the lowest EAB (40.12%) were achieved with 30 mg/g tannin (dry weight). Compared with control, the film containing tannin showed decreased water solubility, water vapor permeability, and oxygen permeability. At the concentrations of tannin studied, the minimum values for water vapor permeability, water solubility, and oxygen permeability were 1.49×10-11 g/(Pa?s?m), 27.76%, and 21.63 Meg/kg, respectively. In addition, differential scanning calorimetry (DSC) analysis indicated that the thermal stability of tannin-gelatin films was also enhanced. Moreover, Fourier transform infrared spectroscopy (FT-IR) analysis showed physical crosslinking effect between tannin and gelatin, dominated by hydrogen bonding and hydrophobic bonding. Thus, the tannin-gelatin film shows potential development value in the field of food packaging.
    39  Changes in Lipolysis, Lipid Oxidation, and Volatile Flavor Compounds during the Processing of Red Braised Pork
    SHI Xiao-na HUANG Feng ZHANG Liang ZHOU Fang-yi ZHAO Zhi-lei ZHANG Chun-jiang ZHANG Hong
    2017, 33(3):257-265. DOI: 10.13982/j.mfst.1673-9078.2017.3.039
    [Abstract](775) [HTML](246) [PDF 563.68 K](1061)
    Abstract:
    Changes in the basic physical and chemical indices, lipolysis, lipid oxidation, and volatile flavor compounds were measured in red braised pork at seven key points during the preparation process: raw meat, wine marinade, frying, stewing over high heat for a half hour, stewing over low heat for a half hour, stewing over low heat for an hour, and finished red braised pork. The water content in the finished pork was found to be significantly decreased (by 10.5%, p<0.05) compared with that in the raw meat. The pH did not change significantly (p>0.05) throughout the process, and reached a maximum value of 6.29 in the finished pork. The salt content reached a maximum of 0.73% in the final product. The thiobarbituric acid values increased significantly during stewing at high heat for a half hour (p<0.05), then decreased, and thereafter increased significantly (p<0.05) to a value of 0.62 mg MDA/kg in the finished pork. Linoleic acid (C18:2), palmitic acid (C16:0), and stearic acid (C18:0) were the primary fatty acids in the meat, and the overall saturated fatty acid content showed a decreasing trend over the course of processing. There were 86 kinds of volatile flavor compounds identified during processing, of which aldehydes, lipids, and furan were the most important in red braised pork.
    40  Distribution and Molecular Typing (MLST) of Non-O157 Diarrheagenic Escherichia coli Isolated from Retail Foods in South China
    ZHANG Shu-hong YANG Guang-zhu LAI Ze-bing WU Qing-ping ZHANG Ju-mei
    2017, 33(3):266-273. DOI: 10.13982/j.mfst.1673-9078.2017.3.040
    [Abstract](829) [HTML](262) [PDF 517.24 K](965)
    Abstract:
    To investigate the distribution and characteristics of non-O157 diarrheagenic Escherichia coli (DEC) contamination in retail foods in south China, food samples from 12 cities in this region were collected randomly and examined using the GB/T 4789.36-2003 method, and the molecular identification of DEC was conducted using multiplex polymerase chain reaction. In addition, multilocus sequence typing (MLST) and the paper disk method were used to analyze the genetic characteristics and drug resistance of DEC strains. The results showed that DEC was detected in 164 samples from 1000 food samples, with a total contamination rate of 16.4%. Severe contaminations were found in meat and aquatic products. Of the five DEC pathotypes tested, enteropathogenic E. coli showed the highest detection rate (8.0%), followed by enterotoxigenic E. coli (6.2%), enteroinvasive E. coli (3.4%), and Shiga toxin-producing E. coli (0.4%). A total of 207 DEC isolates was obtained, and the MLST results showed 58 different sequence types (STs), including 44 reported STs and 14 novel STs. Cluster analysis indicated that eight clonal complexes (CCs) were obtained in these isolates, among which CC10 was the largest. The DEC isolates showed high resistance to tetracycline (72.5%), trimethoprim-sulfamethoxazole (66.2%), cephalothin (50.7%), ampicillin (56.5%), and chloramphenicol (48.3%). The results of this study indicate that related agencies should strengthen the monitoring of non-O157 DEC to reduce the risk of outbreaks of foodborne diseases.
    41  Determination of Total Iron and Ferrous Contents in Brown Sugar by Triple-wavelength Spectrophotometry
    LI Jin-jin ZHAO Bi-qiu NONG Li-zhong CAI Bi-yun HUANG Li-xin
    2017, 33(3):274-278. DOI: 10.13982/j.mfst.1673-9078.2017.3.041
    [Abstract](796) [HTML](528) [PDF 361.36 K](1045)
    Abstract:
    Determination of the total iron content in foods by atomic absorption spectrometry (the national standard method used) requires ashing during pretreatment, which renders the entire operation tedious and time and energy consuming. Herein, a triple-wavelength spectrophotometric method was established for determining the iron content in brown sugar. Brown sugar solutions of known concentration were mixed with phenanthroline monohydrate and then measured by triple-wavelength spectrophotometry at wavelengths of 423, 510, and 700 nm. The total iron content was then calculated from the spectral data. Using this method, the recovery rate of total iron content in brown sugar was evaluated, as well as the content of ferrous iron in brown sugar solutions lacking the reducing agent. The results showed that this method can efficiently deduce the influence of the color agent and baseline drift. The content of total iron was (3.65±0.29) mg/kg, whereas that of ferrous iron was (13.29±0.18) mg/kg. The valence state of iron in brown sugar was mainly ferrous, accounting for more than 98%. The recovery rate of the total iron content was about 103%. This simple technique, which avoids the complicated processes of the traditionally used method, is suitable for industrial applications.
    42  Development of a Colloidal Gold Immunochromatographic Strip for the Rapid Determination of Dimethyl Phthalate
    ZHU Fan DENG Li-Hua ZHU Bin YANG Jin-Yi XU Zhen-Lin SUN Yuan-Ming WANG Hong SHEN Yu-Dong
    2017, 33(3):279-284. DOI: 10.13982/j.mfst.1673-9078.2017.3.042
    [Abstract](770) [HTML](213) [PDF 518.00 K](1058)
    Abstract:
    Phthalate ester plasticizer, a type of environmental hormone that is toxic to reproductive systems, has become a serious health threat because of its illegal transport and addition into foods intended for human consumption. Thus, development of an in situ testing method for the rapid detection of the plasticizer is essential. Herein, a colloidal gold-labeled immunochromatographic test paper method was developed for the rapid detection of dimethyl phthalate (DMP). To prepare the gold-labeled antibodies, anti-DMP polyclonal antibodies were labeled with colloidal gold prepared by the reduction of HAuCl4 using trisodium citrate. The chessboard method and the single-factor test were used to determine the optimal coating concentration and secondary antibody concentration, which were revealed to be 3000 μg/mL and 1600 μg/mL, respectively. The antibody was diluted 1.4-fold. The limit of detection of the developed assay was 0.4 μg/mL, and the visual detection time was within 5 min. Cross-reaction experiments showed no interference from substances with similar structures, indicating the high specificity of the method. The results from strip testing of baijiu liquor were consistent with those from GC-MS. This proposed immunochromatographic assay is fast, simple, and reliable, providing a valid method for the rapid in situ detection of DMP in foods.
    43  Isolation and Identification of the Flavones from Fuzhuan Tea
    LU Ying ZHONG Xiao-hong CAO Jun-xi LIN Hai-yan CHEN Dong LIU Zhong-hua
    2017, 33(3):285-294. DOI: 10.13982/j.mfst.1673-9078.2017.3.043
    [Abstract](949) [HTML](257) [PDF 653.62 K](996)
    Abstract:
    A method for the preparative separation of flavone compounds from Fuzhuan tea using high-speed counter-current chromatography coupled with high-performance liquid chromatography (HSCCC-HPLC) is established in this study. The n-butanol-ethyl acetate-cetonitrile-0.5% acetic acid (12:2:3:15,V/V/) was used as the solvent system for HSCCC, and seven fractions were first separated by HSCCC. Subsequently, each fraction was isolated by the preparative HPLC and 19 compounds were obtained. Fifteen compounds were identified by the spectral methods and all of them were flavonoids: apigenin-bis-6,8-C-β-D-glucopyranoside (1), apigenin-6-C-α-L- arabinopyranosyl-8-C-β-D-glucopyranoside (2), apigenin-7-O-β-D-galactopyranosyl-8-C-β-D-glucopyranoside (3), quercetin-3-O-β-D- glucopyranosyl-(1-3)-L-rhamnopyranosyl-(1-6)-β-D-glucopyranoside (4), kaempferol-3-O-β-D-glucopyranosyl-(1-3)-α-L- rhamnopyranosyl- (1-6)-β-D-glucopyranoside (5), apigenin-7-O-α-L-rhamnopyranosyl -8-C-β-D-galactopyranoside (6), rutin (7), kaempferol-3 -O-α-L- rhamnopyranosyl-(1-6)-β-D-glucopyranoside (8), camelliquercetiside A (9), myricetin-3-O-β-D-glucopyranoside (10), isovitexin (11), vitexin (12), quercetin-3-O-β-D-glucopyranoside (13), camelliquercetiside C (14), kaempferol-3-O-β-D-galactopyranoside (15). Among them, compound 3 and compound 6 were new C-glycosyl flavones and compound 1 was isolated from tea for the first time.
    44  Prediction of Changes in Microbial Composition of Chilled Yellow Broiler Chicken Meat Using HS/SPME-GC/MS Technology
    CHEN Peng CHENG Jing-rong YAGN Yu-xin FANG Shao-qin LIU Xue-ming
    2017, 33(3):295-303. DOI: 10.13982/j.mfst.1673-9078.2017.3.044
    [Abstract](1608) [HTML](190) [PDF 778.31 K](951)
    Abstract:
    The dynamic changes in microbial composition of chilled yellow broiler chicken meat were predicted by assessing changes in volatile metabolic compounds. Samples were packed in trays and stored at -1 and 4 ℃, and sensory evaluation, microbial counts (total viable counts of Enterobacteriaceae spp., lactic acid bacteria, Pseudomonas spp., and Brochothrix thermosphacta), and volatile metabolic compounds were analyzed. Sixty-one compounds were identified in headspace/solid phase microextraction-gas chromatography/mass spectroscopy analysis. To reflect spoilage of chilled yellow broiler chicken under two temperature conditions simultaneously, substances at very low or non-detectable amounts were removed, after which 24 compounds were obtained. The results of principal component analysis showed that 2-butanone, 2-octyl-1-dodecanol, undecane, dodecane, ethyl caproate, ethyl caprylate, anisyl butyrate, 1-nonanal, and trichloromethane were descriptive indicators of freshness, whereas acetoin, 3-methyl-1-butanol, 3-methyl-l-butyraldehyde, 2-ethylhexanol, 2-amino-6-methylbenzoic acid, 2-pentanone, dimethyl disulfide, and ethyl acetate were descriptive indicators of spoilage. Partial least squares regression analysis revealed that these 17 compounds could effectively predict the counts of different microorganisms.
    45  Detection of Rot and Ripeness of Mango with zNoseTM Electronic Nose
    PU Hong-jie WANG Di-song WANG Hui LI Zhen-feng
    2017, 33(3):304-310. DOI: 10.13982/j.mfst.1673-9078.2017.3.045
    [Abstract](983) [HTML](232) [PDF 567.34 K](967)
    Abstract:
    Mango volatiles released during postharvest ripening were quantitatively measured and analyzed with an ultrafast gas chromatography system (zNoseTM). The mango volatiles were characterized by extracting the mass spectral peaks of different volatiles and mathematical models were established to measure the blackspot rate of mango skin and degree of ripeness. Odor, skin blackspot rate, degree of ripeness, soluble solid content, respiration rate, and other experimental data were compared and analyzed. The results showed that peaks 4 and 5 from the electronic nose data could effectively determine the degree of rot, peak 7 exhibited a high correlation with the degree of ripeness, and the soluble solid content showed a small contribution to the determination of the degree of ripeness. Gaussian models were constructed between the degree of rot and values of peaks 4 and 5. The rot of mango was determined using established models and the threshold value and accuracy rate of determination was over 90%. A piecewise exponential model was constructed between the degree of ripeness and value of peak 7, an effective estimation on the degree of ripeness was achieved. The root mean square error of the estimation was controlled within 7%.
    46  Determination of Nine Phytotoxins in Botanical Beverage by Solid-phase Extraction and Gas Chromatography-mass Spectrometry
    ZHOU Si DENG Long DU Wei-feng HE Min-heng XIAN Yan-ping WU Chong-yue HUANG Bao-hua GUO Xin-dong
    2017, 33(3):311-316. DOI: 10.13982/j.mfst.1673-9078.2017.3.046
    [Abstract](751) [HTML](234) [PDF 643.55 K](878)
    Abstract:
    A sensitive method was established for the simultaneous determination of nine phytotoxins, including thujone, estragole, pulegone, safrole, methyl eugenol, coumarin, santonin, and α-and β-asarone, in botanical beverage by solid-phase extraction (SPE) and gas chromatography-mass spectrometry (GC-MS). Samples were extracted and purified using hydrophilic lipophilic balanced (HLB) SPE cartridges after high-speed centrifugation. Finally, the eluent was concentrated by nitrogen gas blowing and all samples were separated using DB-5MS capillary chromatographic column and detected by GC-MS with selected ion monitoring (SIM). The results indicated good linear relationships in the range of 5~1000 μg/L for the nine phytotoxins, with correlation coefficient >0.999. The limits of detection (LODs, S/N=3) and quantification (LOQs, S/N=10) of the method for the target compounds were in the range of 0.3~1.4 and 1.0~4.7 μg/kg, respectively. The mean recoveries of negative samples at three spiked levels were between 76.3% and 99.7%, and the relative standard deviation (RSD, n=6) was <8.7%. This method was suitable for the identification and quantification of nine phytotoxins in botanical beverage owing to its simplicity and accuracy. Among eight actual samples tested, one sample of herb beverage was found containing 0.7 mg/kg methyl eugenol .
    47  Identification of the Characteristic Compounds in Different Series of Liquors Based on HS-SPME-GC-MS Metabolomics
    TIAN Hong XIONG Yue-feng BAO Bin CHEN Shan-qiao WU Wen-hui ZHANG Jing-yi NI Ling TIAN Xiao-qing
    2017, 33(3):317-322. DOI: 10.13982/j.mfst.1673-9078.2017.3.047
    [Abstract](753) [HTML](162) [PDF 537.16 K](778)
    Abstract:
    The headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) detection method and metabolomics data processing technology were creatively applied in the identification of characteristic compounds in liquor and the assessment of liquor adulteration. Seventeen liquor samples were processed through headspace solid phase microextraction, and the volatile compounds in the liquor samples were accumulated. The dimension reduction of the GC-MS data set was achieved using the metabolomics technique. The clustering analysis was employed to correctly distinguish between contaminated and authentic liquors. The classification results from other series of liquors were generally consistent with actual liquor samples. In a principal component analysis, PC1 and PC2 were extracted, and accounted for 58.7% of the variance in the characteristic variables of different liquor samples. Among them, 44.7% of variance was explained by PC1, and a clear distinction was observed between the Hotelling’s elliptical regions of authentic and contaminated liquor samples. The information for authentic liquor samples was maintained in other samples. A liquor related model was established based on a partial least squares discriminant analysis for the removal of base liquor samples, so that the substance difference between authentic and contaminated liquor samples became significant. A variable importance table was generated and 12 related substances were obtained from the database. The top three characteristic compounds in terms of significant difference were butyl acetate, isoamyl caproate, and hexanoic acid isopropyl ester.
    48  Rapid Detection of Salmonella Using Carbohydrate Microarrays
    ZHANG Bei-Lei WANG Xiao-Li WANG Xue-Yu HU Jing YIN Jian
    2017, 33(3):323-327. DOI: 10.13982/j.mfst.1673-9078.2017.3.048
    [Abstract](748) [HTML](171) [PDF 547.22 K](1233)
    Abstract:
    The applicability of carbohydrate microarrays for Salmonella detection was investigated, using Salmonella ATCC31685 as the primary test subject. Different types of carbohydrate microarrays were first tested to optimize the detection conditions. Then, using the optimized conditions, decreased carbohydrate concentrations or bacterial concentrations were used to determine the microarray’s limit of detection. The carbohydrate microarrays were tested for their destructiveness of bacterial strains as well as their ability to screen for antagonists of Salmonella lectin FimH. The results indicated that Salmonella bound to modified mannose compounds in the carbohydrate microarrays, among which the microarray chips with Man-1 and NHS were the most optimal. In addition, the microarray-binding abilities of two Salmonella strains (ATCC31685 and ATCC9184) were found to be different. Therefore, carbohydrate microarrays can serve as a rapid procedure for detecting Salmonella. At a carbohydrate concentration of 313 mmol/L or a cell concentration of 106/mL, the fluorescent signal approaches a background value. The carbohydrate microarray detection of Salmonella is non-destructive and can be used continuously. In addition, these microarrays can be used as tools for screening FimH protein antagonists.
    49  Progress and Prospects of Studies of Chilled Chicken Meat Quality and Shelf Life
    LIU Xue LIU Jiao ZHOU Peng LI Wen-kang ZHANG Xiao-shuan FU Ze-tian
    2017, 33(3):328-340. DOI: 10.13982/j.mfst.1673-9078.2017.3.049
    [Abstract](1053) [HTML](227) [PDF 667.58 K](2380)
    Abstract:
    Compared with fresh and frozen chicken meats, chilled chicken meat has better taste, flavor, and freshness, and thus a large potential market. However, chilled chicken meat is easily spoiled during production, processing, and distribution. With increasing awareness of food quality and safety, consumers are paying increased attention to the quality and shelf life of chilled chicken meat. Here, quality indicators of chilled chicken meat are summarized. The effects of microbial, physical, and chemical factors on the quality of chilled chicken meat are described. The effects and characteristics of chemical treatment, ultra-high pressure treatment, irradiation treatment, and three other types of fresh-keeping techniques on the preservation of chilled chicken meat were compared. In combination with the predictive model of food shelf life, related studies examining the shelf life of chilled chicken meat are reviewed. Finally, the present status and outlook regarding future trends of studies evaluating the quality and shelf life of chilled chicken meat are discussed. Our results suggest that with the continuous development of research techniques, methods, and preservation technology, studies of the shelf life of chilled chicken meat will continue to improve.

    Editor in chief:李琳

    Inauguration:现代食品科技

    International standard number:ISSN 1673-9078

    Unified domestic issue:CN 44-1620/TS

    Domestic postal code:46-349

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