Volume 33,Issue 12,2017 Table of Contents

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  • 1  Study on Acute Toxicity of Bisphenol A to Caenorhabditis Elegans
    ZHOU Xing-hua ZHANG Xiao-wei ZHANG Fei ZHOU Yue XIAO Xiang ZHAO Yan-sheng ZHU Ying ZHANG Jia-yan DONG Ying
    2017, 33(12):1-5. DOI: 10.13982/j.mfst.1673-9078.2017.12.001
    [Abstract](776) [HTML](197) [PDF 436.14 K](952)
    Abstract:
    Bisphenol A (BPA) is a ubiquitous endocrine disrupter that can have side effect on human health. In daily life, people are exposed to low concentration of BPA, while the effect of BPA toxicity is not clear at low concentrations. In order to understand the toxic effects of BPA at low concentration, we used Caenorhabditis elegans (C. elegans) as a model organism to study the acute toxicity of low concentration of BPA. The results showed that the LC50 of BPA was 440.16 mg/L for C. elegans, while the low concentration of BPA had significant inhibitory effect on the movement behavior indexes (including head thrashing frequency and body bending frequency) of C. elegans. The most sensitive index was the head thrashing frequency and the lowest concentration was 1 μmol/L. Low concentration of BPA also affected the growth and development of C. elegans as well as the number of progenies. Compared with the control group, BPA at the concentration of 100 μmol/L reduced body length and progeny by 6.05% and 15.24%, respectively. When the concentration of BPA reached 0.01 μmol/L, it could significantly inhibit the life span of C. elegans and increase the level of ROS, causing oxidative stress injury, accelerating the senescence of C. elegans and shortening its life span. In conclusion, low concentration of BPA exposure could affect the movement, growth, reproduction and longevity of C. elegans.
    2  Effects of 6-shogaol on H2O2-Induced Oxidative Damage in NCM460 and HCT116
    WANG Yu-feng CHEN Chao YANG Yin-xue GONG Fa-hai SUN Shu-hao LI Hai
    2017, 33(12):6-15. DOI: 10.13982/j.mfst.1673-9078.2017.12.002
    [Abstract](1015) [HTML](305) [PDF 891.94 K](1199)
    Abstract:
    In this study, the model of oxidative damage in colorectal cancer cells was established. After the stimulation of hydrogen peroxide (H2O2), the different effects and possible molecular mechanisms of 6-shogoal on H2O2-induced oxidative damage in human intestinal epithelial cells (NCM460) and in situ colon cancer cells (HCT116) cells in vitro were investigated. Inverted microscope was used to observe the changes of cell morphology in HCT116 and NCM460 induced by H2O2 with different concentrations of 6-shogoal. CCK-8 (Cell Counting Kit-8) method was used to screen the concentration interval of 6-shogaol and the cell survival rate was determined. Annexin and V-FITC/PI cytometry were used to detect apoptosis of different groups. The expressions of related apoptotic proteins (Caspase-3, poly (ADP- ribose) polymerase-1 (PARP-1), MCC1, A2F, B-cell lymphoma-2 (Bcl-2)) were detected by Western-blot. As compared with the model group, 6-shogaol could reduce the expressions of caspase-3, PARP-1, MCC-1, A2F and promote the expression of Bcl-2 (p < 0.05) in NCM460 induced by H2O2, indicating that 6-shogaol had anti-oxidant and pro-proliferative effects (p<0.05). However, 6-shogoal could promote the expression of caspase3, PARP-1, MCC1 and A2F, and inhibit the expression of Bcl-2 (p<0.05) in HCT116 induced by H2O2, indicating that 6-shogaol could enhance the oxidative damage of HCT116 induced by H2O2 and inhibit cell proliferation (p<0.05). It could be concluded that 6-shogaol had different opposite effects in NCM460 and HCT116 induced by H2O2, which might provide guidance for further studies on specific mechanisms or pathways of 6-shogoal against colorectal cancer.
    3  Phase Behavior, Texture, and Microstructure Properties of Gelatin-Modified Starch Blend Gels in Co-Solute of Sucrose
    ZHU Jian-hua ZOU Xiu-rong LIU Ri-bin SHAN Bin SUN Chu-xia
    2017, 33(12):16-22. DOI: 10.13982/j.mfst.1673-9078.2017.12.003
    [Abstract](817) [HTML](312) [PDF 759.56 K](1124)
    Abstract:
    The effects of sucrose as co-solute on the phase behavior, texture, dehydration, transparency value L*and microstructure properties of gelatin-modified starch solution blend gels were investigated. The results showed that compared with the control samples,, the hardness and adhesiveness values of blend gels prepared without drying dehydration were reduced significantly (p<0.05) by 21.47% and 13.36% respectively when 16% sucrose was added. There was a non-synergistic effect of sucrose concentration and drying time on the hardness and adhesion value. As the sucrose concentration increased, the drying dehydration rate (-dMg/dt) of blend gels was attenuated significantly, while the L* of blend gels was showed a descending trend. Besides, the average diameter values of concave hole of blending gel microstructure were significantly decreased (p<0.01) with the increasing concentration of sucrose. Compared with the control samples, the average pore diameters decreased from 51.28±1.31 μm to 46.51±1.89 μm in the presence of 16% sucrose and the pore distribution changed from irregular distribution to more dense and uniform. The results also showed that the L* and adhesiveness values had a strong linear relationship with pore average diameters, and the correlation coefficient R2 was 0.98 and 0.97 respectively.
    4  Evaluation of the Lipid-lowering Effect of Mulberry Leaf Ultrafine Powder Milk Tea
    FANG Tai-song ZHU Shuai DING Xiao-wen HUANG Xian-zhi
    2017, 33(12):23-28. DOI: 10.13982/j.mfst.1673-9078.2017.12.004
    [Abstract](891) [HTML](258) [PDF 433.76 K](860)
    Abstract:
    In this study, the hypolipidemic effect of mulberry leaf ultrafine milk tea was investigated to provide reference for the research and development of hypoglycemic and hypolipidemic health food. The effect of mulberry leaf ultrafine milk tea on the body weight and serum lipid related indicators of diabetic mice were investigated by intragastric administration of mulberry leaf ultrafine milk tea with different dosages to the diabetic mice modeled by alloxan. The results showed that the slow growth of body weight in diabetic mice was significantly improved after the feeding of mulberry leaf ultrafine milk tea, and the weight growth rates of female mice and male mice were 37.11% and 40.99%, respectively. After oral administration of the mulberry leaf ultrafine milk tea with different dosages, the levels of total cholesterol (TC), triglyceride (TG) and low density lipoprotein cholesterol (LDL-C) in diabetic mice were significantly decreased by 17.52%, 48.13% and 2.47% respectively, and the high density lipoprotein cholesterol (HDL-C) level was increased by 46.81%. The results showed that mulberry leaf ultrafine milk tea could alleviate the weight loss symptoms of diabetic mice, and the mulberry leaf ultrafine milk tea had certain hypolipidemic effect. The mechanism of hypoglycemic effect of mulberry leaves superfine milk tea on diabetic mice of different genders and the best intake remain to be further research, and it is expected that the study will be able to provide reference for the development of related health food.
    5  The Virulence Genes, Antimicrobial Susceptibility, and CRISPR Molecular Typing of Escherichia coli O157 from Retail Foods in China
    YANG Guang-zhu ZHANG Shu-hong HUANG Yuan-bin WU Qing-ping ZHANG Ju-mei
    2017, 33(12):29-37. DOI: 10.13982/j.mfst.1673-9078.2017.12.005
    [Abstract](755) [HTML](192) [PDF 600.08 K](1352)
    Abstract:
    Escherichia coli O157 is an important food-borne pathogen. 39 E. coli O157 strains identified during 2014 and 2015 in retail foods in china were identified by double PCR to understand the biological characteristics of food-borne E. coli O157. The virulence genes and antimicrobial susceptibility of isolates were also detected, and the genetic diversity of Escherichia coli O157 was analyzed by Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) typing. The results showed that 8 were identified as O157:H7 and 31 were O157 among the 39 strains. , Eae gene was detected in all the isolates, and stx2 was in 82.50% strains of isolates, while stx1 was not detected in the tested 11 virulence genes. Other virulence genes such as espA, etpD, tir, toxB, iha, and katP were detected with the carrying rate of 92.31%, 94.87%, 87.18%, 79.49%, 69.23%, and 46.15%, respectively. Antimicrobial susceptibility results indicated that the isolates strains were highly resistant to TE, SXT, S, C, and AMP. More than 30% of the isolates were resistance to three and more antibiotics. The CRISPR typing indicated that these isolates strains had a high genetic diversity. Thirty-three of the 39 strains had CRISPR1 loci, including 8 E. coli O157:H7 and 26 E. coli O157. The 8 E. coli O157:H7 produced the same spacer maps, while 26 E. coli O157 produced 13 diversified spacer spectra. This study can provide important basic data for food borne disease surveillance, disease traceability and epidemiological research.
    6  Antioxidant and Anti-inflammatory Activities of Polysaccharide from Zizyphus jujuba Mill
    ZHAN Rui SHAO Jin-hui
    2017, 33(12):38-43. DOI: 10.13982/j.mfst.1673-9078.2017.12.006
    [Abstract](849) [HTML](262) [PDF 504.15 K](1190)
    Abstract:
    In this study, antioxidant activity of a jujube polysaccharide from Zizyphus jujuba Mill was investigated by establishing 4 kinds of in vitro models, including 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and hydroxyl radical (?OH) scavenging activities as well as reducing power assay. The model of macrophage RAW264.7 induced by lipopolysaccharide (LPS) was established to study the anti-inflammatory activity of jujube polysaccharide. The results showed that jujube polysaccharide had strong antioxidant activity and the IC50 of jujube polysaccharide on the DPPH?, ABTS and ?OH free radicals were 0.9, 2.8 and 1.1 mg/mL, respectively. The antioxidant activity of jujube polysaccharide exhibited a dose-dependent manner. When the value of total reducing power reached 1.0, the corresponding Vc and jujube polysaccharide concentrations were 0.08 mg/mL and 2.95 mg/mL, respectively. High doses of jujube polysaccharide could significantly reduce the levels of inflammatory cytokines including cyclooxygenase-2 (COX-2), tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) and interleukin-6 (IL-6) in RAW264.7 cells, which showed that jujube polysaccharide had strong anti-inflammatory activity. Jujube polysaccharide was expected as an antioxidant and anti-inflammatory agents used in functional food and medicine industry.
    7  Study on the Correlation between Contents of Polyphenols, Flavonoids, Polysaccharides in Pinus koraiensis Seed Putamina and Their Antioxidant Activities
    ZHAO Nan-nan ZHU Xiao-ran LI De-hai
    2017, 33(12):44-49. DOI: 10.13982/j.mfst.1673-9078.2017.12.007
    [Abstract](717) [HTML](240) [PDF 466.73 K](1498)
    Abstract:
    In order to investigate the correlation between contents of major active ingredients in Pinus koraiensis seed putamina and its antioxidant activity, Yichun Pinus koraiensis seed putamina was used as raw material in this study to extract polyphenols, flavonoids and polysaccharides by different ethanol concentrations. The correlation between active ingredients in Pinus koraiensis seed putamina and their antioxidant activities were analyzed by SPSS statistical analysis software. The results showed that when the concentration of ethanol was 40%, the highest yields of polyphenols, flavonoids and polysaccharides in Pinus koraiensis seed putamina were 4.05 mg/g, 1.7 mg/g and 7.05 mg/g, respectively. Antioxidant activity test showed that antioxidant activity of polyphenols from Pinus koraiensis seed putamina was significantly higher than that of flavonoids and polysaccharides, which was comparable to that of Vc. The IC50 (half clearance concentration) values of polyphenols from Pinus koraiensis seed putamina on the 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical (·OH) and the total reducing power were 0.20±0.05 μg/mL, 13.04±0.04 μg/mL and 40.15±0.06 μg/mL, respectively. The correlation analysis showed that the correlation between the polyphenols from Pinus koraiensis seed putamina and the antioxidant activity was the highest, and the correlations between the polyphenols from Pinus koraiensis seed putamina and DPPH,·OH as well as the total reducing power were 0.908, 0.989 and 0.989 (p<0.01), respectively. It could be seen that polyphenols from Pinus koraiensis seed putamina were the main antioxidant components. The results of this study provided a scientific basis for the comprehensive utilization of the by-products of the Yichun Pinus koraiensis seed putamina and the development of natural antioxidants.
    8  Structural Identification of Phenolic Constituents from Phyllanthus emblica L. and Their Inhibitory Activity on α-glucosidase
    LIU Wei LI Ming-xi WANG Jun-long WAN Chun-peng
    2017, 33(12):50-55. DOI: 10.13982/j.mfst.1673-9078.2017.12.008
    [Abstract](713) [HTML](252) [PDF 547.74 K](824)
    Abstract:
    The phenolic constituents in the hypoglycemic active fraction of the methanol extract of Phyllanthus emblica L and their inhibitory activity on α-glucosidase were studied. Using methanol soaking extract Phyllanthus emblica L, the methanol extract (PE) was chromatographed on a D101 macroporous resin column to obtain three fractions (PEA-PEC). Hypoglycemic activity test showed that 30% ethanol elution part (PEA) was the active fraction. PEA was further isolated and purified by column chromatography of Sephadex LH-20, ODS and semi-preparative HPLC columns. The structures of the isolated compounds were identified according to the physicochemical properties, 1H-NMR, 13C-NMR and high resolution mass spectral analyses as well as relevant references. The compounds were tested for their in vitro inhibitory activity on α-glucosidase. Seven phenolic compounds were isolated from PEA fraction and identified as gallic acid (1), methyl gallate (2), 1-O-galloyl-β-D-glucose (3), 1,6-di-O-galloyl-β-D-glucose (4), corilagin (5), mucic acid-1,4-Lactone-2-O-gallate (6), mucic acid-2-O-gallate (7). All compounds except compound 2 showed good inhibitory activity on α-glucosidase. Gallic acid derivatives were the active constituents that P. emblica L inhibited the activity of α-glucosidase.
    9  Antimicrobial Activity of Polyphenols from Black Chokeberry
    MIAO Miao LIU Yu-xuan HU Miao-miao YUE Tian-li YUAN Ya-hong CAI Rui WANG Zhou-li
    2017, 33(12):56-60. DOI: 10.13982/j.mfst.1673-9078.2017.12.009
    [Abstract](736) [HTML](201) [PDF 371.32 K](874)
    Abstract:
    The antimicrobial activity and antibacterial stability of polyphenols from black chokeberry on five kinds of bacteria and two kinds of fungi were investigated in this study. The bacteriostatic activity of polyphenols from black chokeberry was determined according to the diameter of bacteriostasis zone by double-layer plate perforating method. The minimum inhibitory concentrations (MICs) of polyphenols from black chokeberry against five bacteria were determined by spectrophotometry method. The effects of salt concentration, temperature and UV irradiation on the antibacterial stability of polyphenols from black chokeberry were investigated by using Alicyclobacillus and Bacillus subtilis as indicator bacteria. The results showed that the polyphenols from black chokeberry had good antibacterial activities against five kinds of bacteria, such as Alicyclobacillus and Bacillus subtilis, but no antibacterial effect on the two kinds of fungi. The MIC of polyphenols from black chokeberry against Alicyclobacillus (DSM 3922, AAT-92) and Enterobacter cloacae (20051) was 0.40 g/L, and the MICs of polyphenols from black chokeberry against Bacillus subtilis. (10034) and Enterobacter aerogenes (10017) were 0.05 g/L and 0.10 g/L, respectively. With the increase of NaCl concentration, the antimicorbial activity of polyphenols from black chokeberry decreased significantly. After the heat treatment, the antibacterial activity of polyphenols from black chokeberry decreased slightly, and the antibacterial activity did not change after UV treatment.
    10  Interaction of Saccharomyces Cerevisiae and Acetobacter Pasteurianus in Liquor Brewing
    WU Xuan-de LI Zhou ZHOU Shi-shui
    2017, 33(12):61-67. DOI: 10.13982/j.mfst.1673-9078.2017.12.010
    [Abstract](829) [HTML](191) [PDF 633.47 K](1145)
    Abstract:
    Saccharomyces cerevisiae and Acetobacter pasteurianus were important fermentation microorganisms in the production of liquor brewing, which had a significant effect on the generation of ethyl acetate. The interaction of S.cerevisiae and A.pasteurianus and its effect on the generation of ethyl acetate were investigated in this paper by using synchronized and sequential modes, and taking the S.cerevisiae inoculation as a control. The results indicated that A.pasteurianus had little impact on the growth and metabolism of S.cerevisiae in the early fermentation stage by using synchronized inoculation, and the S.cerevisiae alcoholic fermentation inhabited the growth of A.pasteurianus and the synthesis of ethyl acetate in the mixed fermentation. In the late fermentation stage, the growth and acid production of A.pasteurianus accelerated the decline of S.cerevisiae and the synthesis of ethyl acetate. The highest content of ethyl acetate was 595.72±5.01 mg/L, which was 10.1 times than that of S.cerevisiae fermentation. The A.pasteurianus, inoculated with yeast for 24 h, had the greatest impact on S.cerevisiae alcoholic fermentation by using sequential inoculation .The alcohol content was lower 22.98±1.77% than that of the control, and the content of ethyl acetate in sequential inoculation mode was lower than that in synchronous inoculation mode. In addition, some substances produced by S.cerevisiae metabolism inhibited the synthesis of ethyl acetate by A.pasteurianus, which could be relieved with the presence of living S.cerevisiae in fermentation system.
    11  Comparative Analysis of Antioxidant Activity of Crude Flavonoids in Different Varieties of Ginger
    MEI Xing WANG Qiong TIAN Rui LI Wei
    2017, 33(12):68-76. DOI: 10.13982/j.mfst.1673-9078.2017.12.011
    [Abstract](662) [HTML](248) [PDF 868.43 K](1186)
    Abstract:
    In this paper, the effects of different varieties on the antioxidant activities of crude flavonoids from ginger were mainly compared and analyzed. Using the fengtou tender and old ginger and yellow craw old ginger as the raw materials, the effects of three different flavonoids from ginger on the scavenging effect of superoxide anion free radicals, hydroxyl radical and hydrogen peroxide as well as the protective effect of the DNA-damage were determined by the chemiluminescence methods. The results showed that the antioxidant effect of flavonoids from old ginger was superior to that of tender ginger, but the protective effect of flavonoids from tender ginger was significantly better than that of old ginger in the DNA damage system. In terms of variety, the antioxidant effect of the flavonoids from different varieties in hydrogen peroxide system was not significant, while the differenes of the other three systems reached a significant level. The antioxidant effect of flavonoids from fengtou ginger was better than that of yellow craw ginger in the hydroxyl radical, hydrogen peroxide and DNA damage protection system, but the scavenging effect on the superoxide anion free radical of flavonoids from yellow craw ginger was slightly better than the fengtou ginger. However, the difference was not significant at 0.01 level. A comprehensive study showed that the antioxidant effect of crude flavonoids from ginger was influenced by variety and maturity.
    12  Protein Recovery and DPPH Radical Scavenging Activity of Enzymolyzed Products of Moringa Oleifera Seed
    ZHAO Yi-jun LIU Hua-yong CHEN Sen-lin TAO Hong ZHAO Qiang-zhong
    2017, 33(12):77-83. DOI: 10.13982/j.mfst.1673-9078.2017.12.012
    [Abstract](870) [HTML](243) [PDF 588.57 K](962)
    Abstract:
    The enzymolyzed products of Moringa Oleifera seed were prepared by aqueous enzymatic extraction. The protein recovery and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of the enzymolyzed products were selected as indexes to optimize the enzymatic hydrolysis conditions . Single factor experiment showed that the enzymolyzed products obtained by Alcalas 2.4L and Ns 37071 had the highest protein recovery and DPPH radical scavenging activity. The protein recovery were 45.65% and 47.65%, respectively, and the DPPH free radical scavenging activity were 99.88 and 93.89 μmol Trolox equiv/100 g, respectively. Further, the optimal enzymolysis conditions confirmed by response surface methodology were as follows: the total amount of enzyme 2.60%, Ns37071 accounted for 46% of the total amount of enzyme, and enzyme time 24 hours. The protein recovery was 63.69±2.94%, and DPPH radical scavenging activity was 139.38±0.96 μmol Trolox equiv/100 g. The study in this paper showed that the aqueous enzymatic extraction could efficiently hydrolyze Moringa oleifera seed, and enzymolyzed products had better protein recovery and DPPH radical scavenging activity.
    13  The Antioxidant and Antityrosinase Activities of Carvacrol
    ZHANG Ling-zhi XU Jia SHU Qing-long SHAO Wen-xiang LIU Sheng-zhang ZUO Ai-ren
    2017, 33(12):84-88. DOI: 10.13982/j.mfst.1673-9078.2017.12.013
    [Abstract](933) [HTML](178) [PDF 457.11 K](1039)
    Abstract:
    The antioxidant and antityrosinase activities of carvacrol was investigated by scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2‘-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), hydroxyl radical (?OH) and superoxide radicals, inhibiting lipid peroxidation and inhibiting tyrosinase activity. The experiment results showed that carvacrol had excellent antioxidant and antityrosinase activities in dose-dependent manner. In the experiments of scavenging DPPH, ABTS, ?OH and superoxide radicals, inhibiting lipid peroxidation and inhibiting tyrosinase activity, the IC50 of carvacrol were 100 μmol/L, 18.18 μmol/L, 16 μmol/L, 16 μmol/L, 12.5 μmol/L and 6.67 μmol/L, respectively. The IC50 of kaempferol used as the positive control drug were 80 μmol/L, 9.09 μmol/L, 12 μmol/L, 12 μmol/L, 8.33 μmol/L and 3.33 μmol/L, respectively. Carvacrol had one phenolic hydroxyl group, while kaempferol had three phenolic hydroxyls. The different molecular structures led to the different antioxidant and antityrosinase activities. In a word, carvacrol had good antioxidant and antityrosinase activities, worth being further researched as the new anti-aging candidate drug, cosmetic materials and food additives.
    14  Adsorption Behavior of Ball-milled Chitosan in LiOH Solution
    YU Hong QIU Chao-kun DUAN Dong-ya CHENG Xiao-he ZHANG Wei-ni
    2017, 33(12):89-92. DOI: 10.13982/j.mfst.1673-9078.2017.12.014
    [Abstract](747) [HTML](208) [PDF 10.01 M](1254)
    Abstract:
    Chitosan was ball milled by a planetary ball mill, and the effects of temperature and concentration of LiOH aqueous solution on the adsorption of chitosan in LiOH aqueous solution were studied and characterized by FT-IR spectra and XPRD spectra. The deacetylation degree of chitosan had no change, while the molecular weight of chitosan gradually declined during ball milling. Swelling degree of ball-milled chitosan in LiOH aqueous solution was all higher than that of the native chitosan in LiOH aqueous solution, which illustrated that ball milling treatment could promote the absorption of chitosan in LiOH aqueous solution. The solution temperature and concentration of LiOH aqueous solution had a significant influence on the swelling degree of chitosan and the swelling degree of ball-milled chitosan at 5 ℃ in 4.8wt% LiOH aqueous solution was the highest, which had no significant difference with swelling degree of chitosan in 4.8wt% LiOH solution by freezing-blasting. It illustrated that adsorption of ball-milled chitosan in low-temperature LiOH aqueous solution could also achieve the same effect of dissolution without tedious process of freezing-blasting. XPRD spectra and FT-IR spectra indicated that LiOH destroyed the crystal structure of chitosan and reacted with the acetyl and the hydroxyl groups of chitosan during the swelling process of LiOH solution.
    15  Comparison of Calcium Content in Different Calcium Products by Rapid Calcium-Holding Activity in Vitro and Atomic Absorption Spectrometry
    SUN Sheng-wei GUO Bin MIAO Jian-yin CAO Yong
    2017, 33(12):93-98. DOI: 10.13982/j.mfst.1673-9078.2017.12.015
    [Abstract](705) [HTML](321) [PDF 521.41 K](883)
    Abstract:
    Casein phosphopeptide (CPP), hydrolyzed and separated from milk casein, was a bioactive peptide and had a strong calcium absorption activity. The inhibition of calcium phosphate/calcium carbonate precipitation was investigated by CPP to characterize the calcium-holding effect of CPP in the present study. The calcium-holding activity of three commercial calcium tablets was investigated by rapid demonstration experiments in vitro, and the comparison of calcium content was determined by atomic absorption spectrometry. The results showed that the CPP-containing calcium tablets had an obvious advantage of calcium-holding and prevented calcium phosphate/calcium carbonate from precipitating for more than 30 minutes. In addition, the precipitation of CPP-containing calcium tablets was significantly less than the other two calcium products under the conditions of same calcium tablets content and calcium content while greatly more than the other two calcium tablets after treated with sodium carbonate solution, indicating that the CPP-containing calcium tablets had a significant effect on prolonging time calcium-holding and inhibiting the formation of calcium carbonate precipitation. The results of this study provided a reasonable scientific support for the calcium supplement effect of commercial calcium products and had a great guiding significance to the stable development of the health care industry.
    16  Temperature and Quality Changes of Pasteurized Milk during Refrigerated Storage and sale in Refrigerator
    WU Da-xiong LI Dong-mei LI Song-qun CHEN Sheng-nan
    2017, 33(12):99-103. DOI: 10.13982/j.mfst.1673-9078.2017.12.016
    [Abstract](741) [HTML](238) [PDF 373.43 K](983)
    Abstract:
    In order to investigate the temperature and quality changes of pasteurized milk packaged during normal storage and sale in refrigerator, parameters of sale temperature and time were obtained by measuring the normal storage and sale with the temperature fluctuation changes in refrigerators. The microorganism index, physical and chemical indicators, acidity and the sensory quality were studied in 5 d during the storage and sale in refrigerator, which was compared with the unopened sale as control. The results showed that the protein, fat, lactose, non-fat milk solids contents in the milk samples had not changes under the different storage conditions within the first five days while acidity and the sensory quality were almost the same under different storage conditions during 5 d storage and Coliform, Staphylococcus aureus and Salmonella were not detected. However, the aerobic plate count in the milk samples have been speeded up with the difference statistically significant (p<0.05) to the contrast group during 5 d storage period on sale in refrigerator, and the two group accorded with the requirement of national standard. It indicated that temperature fluctuation had an impact on the quality of pasteurized milk during storage sale in refrigerator. Low storage temperature and relatively short storage time were conducive to the retention of high quality of pasteurized milk. The study also showed that controlling the sale temperature fluctuation in refrigerator was important. To ensure that the sale refrigeration, accumulated time for storage products over 6 ℃ was not more than 7 h and temperature was below 8 ℃;The accumulated time for refrigerator body temperature over 6 ℃, was not more than 9 h and temperature was below 9 ℃.
    17  Inhibitory Effects of Ingredients of Fructus schisandrae on the α-glucosidase Activity
    QIU Ming-yang TIAN Shuang ZHAO Wu LIU Yu-ting GONG Pei-han YAN Ming-ming SHAO Shuai
    2017, 33(12):104-108. DOI: 10.13982/j.mfst.1673-9078.2017.12.017
    [Abstract](952) [HTML](210) [PDF 462.42 K](1005)
    Abstract:
    The inhibitory effects of total lignans, total flavone, total triterpenes, schisandrol B, deoxyschizandrin, schisandrin and schisantherin A of Fructus schisandrae on the α-glucosidase activity. Using the in vitro inhibition of α-glucosidase activity as a model and 4-nitrophenyl α-D-glucopyranoside (PNPG) as substrate as well as acarbose (ACAR) as a positive control, the inhibitory effects of total lignans, total flavone, total triterpenes, schisandrol B, deoxyschizandrin, schisandrin and schisantherin A of Fructus schisandrae on the activity of α-glycosidase were determined in vitro. When the concentrations of total lignan, total flavone and total triterpenes in Fructus schisandrae were 1.0 mg/mL, the inhibitory rates of α-glucosidase were 96.15±1.52%, 60.33±1.84% and 48.62±2.15%, respectively. When the concentrations of schisandrol B, deoxyschizandrin, schisandrin and schisantherin A were of 0.33 mg/mL, the inhibitory rates of α-glucosidase were 96.53±1.62%, 68.48±1.83%, 15.57±2.37%, 6.24±2.49%, respectively. The total lignans and schisandrin B in Fructus schisandrae had a significant inhibitory effect on the activity of α-glucosidase, and had a significant dose-effect relationship.
    18  Physicochemical Property and Antioxidant Activity of Corn Fiber Gum
    FENG Hui-min HUANG Qiang LI Chao
    2017, 33(12):109-114. DOI: 10.13982/j.mfst.1673-9078.2017.12.018
    [Abstract](702) [HTML](196) [PDF 515.60 K](916)
    Abstract:
    Two kinds of corn fiber gum fractions, named CFG1 and CFG2, were extracted from de-oiled and de-starched corn fiber by the alkaline hydrogen peroxide method, and thephysicochemical property, emulsifying capacity and antioxidant activity of CFG1 and CFG2 were investigated. The yields of CFG1 and CFG2 were 16.3 and 5.7%, respectively (based on the dry weight of corn bran). CFG1 and CFG2 showed good thermal stability at 220 ℃ and CFG2 exhibited a higher apparent viscosity. The thermodynamics analysis indicated that both CFG1 and CFG2 had stronger emulsifying activity index and emulsification stability index, and the stable emulsion of CFG2 exhibited higher viscosity. . The antioxidant activity analysis showed that CFG1 and CFG2 had significant DPPH radical scavenging activity and oxygen radical absorption capacity (ORAC) in a dose-dependent manner. The scavenging rates of CFG1 and CFG2 were 71.8 and 83.1%, respectively, at the concentration of 6.0 mg/mL. In addition, the ORAC values of CFG1 and CFG2 were 69 and 120 μmol TE/g, respectively, indicating that CFG2 had stronger antioxidant activity. The DPPH and ORAC results of CFG suggested that CFG (especially for CFG2) could be developed as a natural emulsifying agents with favorable antioxidant activity.
    19  Separation of Bio-antagonists from Sea and the Application in Fruit Postharvest Fresh-keeping
    ZHANG Shu-xuan YUAN Yong-bing ZHANG Xin-fu WANG Yu-ling YANG Shao-lan
    2017, 33(12):115-119. DOI: 10.13982/j.mfst.1673-9078.2017.12.019
    [Abstract](677) [HTML](206) [PDF 517.79 K](834)
    Abstract:
    The effects of bio-antagonists on the prevention of Valsa ceratosperma and the application in fruit postharvest fresh-keeping of apple and pear were analyzed in this paper. The fungus was classified into Galactomyces geotrichum by microexamination combined with molecular biology. The antagonistic effect of fungus on the fruit Valsa ceratosperma was studied based on the plate bacteriostasis and in vivo experiments, which indicated that the fungus could inhibit Valsa ceratosperma and 5×107 CFU/mL of the fungus had good effect on the prevention of pear and apple rot inoculated with 5×104 spores/ml pathogenic bacteria. Compared with the control fruit, the rot rate of pear treated by the fungi solution decreased 20% after storage 20 d and the apple decreased 30% after storage 60 d. Furthermore, the softening of fruit could be delayed and the fruit maintained good nutritional quality after treated by the fungus. The experiments showed that the fungus separated from sea had high potential in biological control of fruit disease and could be applied in the fruit preservative and fresh-keeping.
    20  Preparation and Properties of Terephthaloyl Chloride Cross-linked Esterified Starch
    LIANG Yi-chao GUO Chen-feng ZHANG Ben-shan
    2017, 33(12):120-128. DOI: 10.13982/j.mfst.1673-9078.2017.12.020
    [Abstract](585) [HTML](165) [PDF 654.70 K](835)
    Abstract:
    The preparation method and the product properties of cross-linked esterified corn starch were studied by using terephthaloyl chloride as the crosslinking agent. Single factor experiment was used to optimize the preparation of terephthaloyl chloride cross-linked esterified starch, and the fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and brabender viscometer were used for the characterization of the prepared products. The physicochemical properties of terephthaloyl chloride cross-linked esterified starch such as light transmittance and salt resistance were measured, providing a theoretical basis of the study on terephthaloyl chloride cross-linked esterified starch. The results of infrared absorption spectrum (IR) showed that the characteristic absorption peak appeared at around 1570 cm-1 was attributed to benzene ring, which indicated that the benzene ring was successfully introduced on the cross-linked starch. XRD spectrum showed that crosslinked starch and original starch belonged to A-type structure, and the crystallinity of crosslinked starch decreased slightly, which indicated that crosslinking reaction had an effect on the overall structure of the starch granules. In addition, the crosslinking reaction did not cause serious damage to the crystalline and subcrystalline regions. The optimum preparation conditions were as follows: amount of terephthaloyl chloride 5% of dry starch, amount of sodium hydroxide 4% of dry starch, reaction temperature 45 ℃, reaction time 7 h.
    21  Effects of 1-Methyleyelopropene and Ethylene Treatments on the Respiratory Metabolism and Cell Structure of Postharvest Zizania latifolia
    WANG Wei-hua JIANG Li WANG Li-bin LUO Hai-bo HE Xiong YU Zhi-fang
    2017, 33(12):129-136. DOI: 10.13982/j.mfst.1673-9078.2017.12.021
    [Abstract](864) [HTML](210) [PDF 875.14 K](921)
    Abstract:
    In order to explore the possible mechanism of postharvest senescence in Zizania latifolia, the effects of 10 μL/L 1-methyleyelopropene(1-MCP) and 1.25 mg/L ethylene(ET) treatments on the respiratory metabolism and cell structure associated with senescence in Z. latifolia stored at 25 ℃ for 6 days were investigated. The results showed that the 1-MCP treatment significantly inhibit ed the increase of L, a*, b* value and weight loss of Z. latifolia, whilethe respiratory intensity and reducing sugar content were significantly lower than those in the control. The superoxide dismutase (SOD) activity in samples treated with 1-MCP was significantly induced after 1 day storage but reduced its activity during the remainder storage period. 1-MCP treatment also promoted the cytochrome c oxidase(CCO) activity, while inhibited succinatedehydrogenase (SDH) and peroxidase(POD) activities compared with the control, which resulted in a relatively low relative leakage rate and malondialdehyde(MDA) content. After 6 days storage at 25 ℃, the integrity of cell structure was still maintained. Compared with 1-MCP treatment, ET treatment had the opposite effects on respiratory and reactive oxygen species(ROS) metabolism. These results suggested that 1-MCP treatment could significantly inhibited the respiratory metabolism and ROS production, maintained the energy metabolism balance and cell structural integrity, and then retard the postharvest senescence of Z. latifolia.
    22  Effects of Hot-air Drying as well as Short-and Medium-wave Infrared Radiation Drying on the Drying Characteristics and Polyphenol Content of Peach Pomace
    LI Cong ZHOU Mo BI Jin-feng CHANG Xue-dong CHEN Qin-qin NING Chun-yuan
    2017, 33(12):137-144. DOI: 10.13982/j.mfst.1673-9078.2017.12.022
    [Abstract](1753) [HTML](226) [PDF 710.74 K](933)
    Abstract:
    The drying characteristics, effective moisture diffusivity (Deff) and activation energy (Ea) of peach pomace under hot-air drying (HAD) as well as short- and medium-wave infrared radiation drying (IRD) at 60 ℃, 70 ℃ and 80 ℃ were investigated. The mathematical models of peach pomace under different drying processes were established and the total polyphenol contents of peach pomace under different drying conditions were tested. The results showed that the Midilli et al. model was the best model to describe the drying process of peach pomace under all drying conditions (R2>0.9996) as compared with the five drying models established in this study. The values of effective moisture diffusivity varied from 1.1652×10-9 to 1.7393×10-9 m2/s for HAD and from 1.6718×10-9 to 2.4993×10-9 m2/s for IRD. The activation energies of HAD and IRD methods for peach residue calculated by Arrhenius equation were 19.56 kJ/mol and 19.68 kJ/mol, respectively. In addition, the total polyphenol retention in the IRD samples were 68.22%, 75.42% and 82.63%, which were higher than that in HAD samples at the same drying temperature. In conclusion: the drying rate and polyphenol content of peach pomace under IRD were higher than those under HAD, and the content of polyphenol increased with the increasing drying temperature. The IRD process at 80 ℃ had the minimal effect on the polyphenol content of peach pomace. This study provided the theoretical basis for the drying characteristics of peach pomace under different drying conditions and the usage of polyphenol in the future.
    23  Effects of Dissolved Oxygen on Non-Enzymatic Browning of Litchi Juice during the Storage
    WU Min HU Zhuo-yan
    2017, 33(12):145-154. DOI: 10.13982/j.mfst.1673-9078.2017.12.023
    [Abstract](585) [HTML](297) [PDF 800.46 K](1051)
    Abstract:
    The non-enzymatic browning of litchi juice during storage was investigated under the storage conditions of different initial dissolved oxygen concentrations (DOC) and headspace oxygen. The effects of DOC ion non-enzymatic browning of litchi juice was analyzed by PLSR. Results showed that the changes of L-AA of litchi juice were dominated by aerobic degradation in low and medium DOC with the rapid decrease of DOC during the storage of 0~3d. The DOC decreased slowly and tended to be stable during the storage of 3 d~17 w, and the changes of L-AA were both dominated by aerobic degradation and anaerobic degradation, and mainly by anaerobic degradation. The L-AA of litchi juice in high DOC continued aerobic degradation and almost completely degraded in 4 w with the rapid decrease of DOC and headspace oxygen concentration during the pre-storage. The sucrose of litchi juice degraded constantly under the condition of three DOC whereas the content of fructose and glucose exhibited a trend of rapid increase and then decrease. The 5-HMF and BI increased constantly with the increase of DOC. Epicatechin degraded continuously whereas the content of rutin was relatively stable. The non-enzymatic browning of litchi juice was dominated by L-AA degradation and oxidative polymerization of phenolics with Maillard reaction supplemented in different DOC.
    24  Optimization of Fermentation Conditions for Producing κ-Carrageenase by a Marine Strain Pedobacter sp. NJ-02
    CHEN Xu YAO Zhong YUAN Wei-kang SUN Yun ZHU Ben-wei SUN Yun ZHU Ben-wei
    2017, 33(12):155-161. DOI: 10.13982/j.mfst.1673-9078.2017.12.024
    [Abstract](686) [HTML](279) [PDF 713.31 K](853)
    Abstract:
    A strain NJ-02 with high κ-carrageenan producing activity was acquired through screening and separation, and identified as Pedobacter sp. by physiology biochemistry experiment, 16S rRNA sequences determination and phylogenetic tree analysis. The optimal fermentation conditions were studied based on the single-factor and response surface experiment. The optimal conditions were obtained by single-factor experiment as follows: κ-carrageenan as carbon source (2.00 g/L), yeast extraction as nitrogen source (4.00 g/L), pH 7.00, inoculation amount 5%, temperature 30℃, and table speed 150 r/min. According to the single factor experiments, the carbon resource, nitrogen source, and pH were determined to be three key factors influencing κ-carrageenan enzyme production in fluid fermentation. A regression model for the yield of κ-carrageenan enzyme was established using a Box-Behnken design involving the above three factors with three levels. The best fermentation medium was found as follows: κ-carrageenan 2.15 g/L, yeast extraction 4.32 g/L, and pH 7.10. Under these conditions, the enzyme activity was 19.01 U/mL, which was 1.20 times as that of the original conditions. It provided the experimental foundation and theoretical basis for large quantity of κ-carrageenan and further study.
    25  Effects of Different Drying Methods on the Quality of Pleurotus eryngii Dried Productafter Non-Sulfite Color Protection
    ZHANG Miao-qing CHENG Fei-er CHANG Ming-chang MENG Jun-long LIU Jing-yu FENG Cui-ping
    2017, 33(12):162-169. DOI: 10.13982/j.mfst.1673-9078.2017.12.025
    [Abstract](821) [HTML](189) [PDF 768.22 K](913)
    Abstract:
    Browning problems occurred during the drying process of Pleurotus eryngii, and seriously affected the sensory quality of products. Different Three drying methods of hot air, far infrared and vacuum freeze were used for fresh Pleurotus eryngii after non-sulfur color protection. The results showed no significant difference among the three drying methods on the color of dried products after the treatment of color protection. Rehydration time of Pleurotus eryngii after vacuum freeze-drying was shorter than the other two methods, 2 min. Compared with the hot air-drying, PPO activity of Pleurotus eryngii significantly increased (p<0.01) after the far infrared-drying, and other indexes were not significant. The activities of SOD, CAT, PPO, POD and the viscosity increased significantly after the vacuum freeze-drying (p<0.01), and the hardness, elasticity and chewiness decreased with significant differences (p<0.01). The results of cell microstructure showed that three drying methods induced the cell deformation and the vacuum freeze-drying exhibited least influence. Consequently, the vacuum freeze-drying method had little influence on the quality of Pleurotus eryngii after color protection.
    26  Optimization of Fermentation Medium for Genetically Engineered Strain with Camel Chymosin-producing capability
    LIU Kai-fang WANG Min ZHENG Jun LIU Tian-tian HUI Feng-li
    2017, 33(12):170-176. DOI: 10.13982/j.mfst.1673-9078.2017.12.026
    [Abstract](550) [HTML](162) [PDF 561.31 K](774)
    Abstract:
    Chymosin can specifically cleave the peptide bond between κ-casein Phe 105 and Met 106 in milk, cause milk-clotting and widely used in dairy products. Bovine chymosi has been deeply studied, but few research on camel chymosin were reported. According to the single-factor experiments design, Plackett-Burman design was used to evaluate the effects of 8 medium components on the production of chymosin to obtain a high-performance fermentation medium. The results showed that glucose, corn syrup, and urea concentrations had significant effects on the chymosin production. Box-Behnken experimental design was employed to optimize the three medium components furtherly. The optimal fermentation medium for antifungal substance production was composed of glucose 86.6 g/L, corn syrup 56.9 g/L, yeast extract 4g/L , KH2PO4 3 g/L, urea 18 g/L, ZnSO4 3 g/L, (NH4)2SO4 1 g/L and MgCl2 0.3 g/L. Under the optimal conditions, the activity of chymosin could reach 431.25 SU/mL, which were 2.08 folds higher than the original medium (207.03 SU/mL). The chymosin activity reached 530 SU/mL after fermentation in an 1Lfermentation tank with exact medium components,being 1.22 folds higher than the chymosin activity obtained by shake flask fermentation.
    27  Effects of Different Inner-Packages Treatments on the Quality of Juicy Peach Fruits during Simulated Transportation
    LI Chun-hong ZHOU Hong-sheng ZHANG Lei-gang LUO Shu-fen HU Hua-li LI Peng-xia
    2017, 33(12):177-183. DOI: 10.13982/j.mfst.1673-9078.2017.12.027
    [Abstract](930) [HTML](163) [PDF 706.19 K](888)
    Abstract:
    Mechanical damage is one of the main reasons for the decline of fruits and vegetables quality during transportation, while the inner-package is the key factor for quality of them during the process. In the experiment, the effects of cushioning materials and five inner-packages types on mechanical bruising of packaged peach fruits (the ‘baifeng’ cultivar) were investigated under the conditions of ambient temperature (23±1 ℃) and relative humidity of 70%~80%. In order to screen out the most suitable inner-package for peaches, different distances of express transportation was simulated according to the standard of highway transportation. The results indicated that the inner-package of EPE and film (P5) could retard the reduction of firmness, slow down the decrease of soluble solid content (TSS) and L* value, alleviate browning degree of juicy peach fruits, suppress the increase of weight loss and respiration rate, and remain the higher resistant enzymes activities of APX (ascorbate peroxidase), POD (peroxidase) and CAT (catalase). Moreover, PAL (phenylalanineammonialyase) activities were kept in a relatively low level. Considering the factors of the freshness, flavor and taste, the P5 inner-package exhibited the best capacity for vibration reduction and fresh-keeping of juicy peach fruits. In conclusion, the P5 package method was the most appropriate inner-package treatment for postharvest circulation of juicy peach fruits.
    28  S Study on the Spray Drying Technology for production of Soybean Milk Powder with Low Anti-nutritional Factors
    ZHAO Qiao-li WANG Li LIAO Zhen-lin HU Hui-gang FANG Xiang
    2017, 33(12):184-190. DOI: 10.13982/j.mfst.1673-9078.2017.12.028
    [Abstract](734) [HTML](287) [PDF 626.52 K](928)
    Abstract:
    The spray drying technique for producing soybean milk powder with low anti-nutritional factors was studied and compared with 6 kinds of commercial soybean milk powders. The papain hydrolyzed soybean milk was prepared by traditional raw pulp processing. By investigating the physical characteristics of soybean milk powder, the optimal spray drying processing conditions were determined as follows: inlet temperature of 185 ℃, inlet flow rate of 350 mL/min, the hydrophilic monoglyceride and twain 80 (3:2) dosage of 2.0%, and the maltodextrin dosage of 5.4%. Under these conditions, the wettability and dispersibility were 74.05% and 11.94% higher than those of ice spring soybean milk powder, respectively. The solubility was better than those of other commercial soybean milk powder except for Weiwei and Black Bull branded soybean milk powders. The urease activity was negative, the contents of β-conglycinin, glycinin and phytic acid were lower than that of commercial soybean milk powder, trypsin inhibitor was 15.32% lower than that of ice spring soybean milk powder, and soybean agglutinin was 14.00% lower than that of Yonghe soybean milk powder. The data indicated that the soybean milk powder produced by the process had good flavor and solubility, and the contents of glycinin, β-conglycinin and phytic acid were significantly lower than that of commercially soybean milk powder.
    29  Effect of Cultivation Altitudes and Brewing Processes on the Aromas and Phenolic compounds in Wine
    WEI Xiao-feng MIN Zhuo HE Shuang CAO Jian-hong MA Xue-lei FANG Yu-lin
    2017, 33(12):191-201. DOI: 10.13982/j.mfst.1673-9078.2017.12.029
    [Abstract](712) [HTML](215) [PDF 692.87 K](907)
    Abstract:
    Using the stir bar solid phase extraction coupled with gas chromatography-mass spectrometry, the aromas and phenols inwines of 6 different cultivation altitudes and 2 brewing processes from Shangri-La area were analyzed. The results showed that the content of residual sugar in wine decreased with the increase of altitude, but the difference was not significant. Within 500 meters of the altitude range, the contents of total phenols, total flavonoids and total anthocyanins increased about 300 mg/L, 700 mg/L and 100 mg/L, respectively, while contents of total tannin and flavanols showed a slight downward trend with the increase of altitude. The total amount of monophenols, catechin, quercetin (3~20 mg/L) and most of the monomer anthocyanins (1-15 mg/L) showed varying degrees of increase. The contents of aldehydes, ketones and total aroma substances in wine from the highest altitude were significantly higher than those in wine from other altitudes. Esters content in the wine from the highest altitude was significantly lower than that in wine sample from the lowest altitude. The percentages of total flavanols, total anthocyanins and total tannin in Xidang-A sample were higher than those in Xidang-B. Percentages of esters (61.45%), aldehyde ketone and terpenes (0.59%) in Xidang-A sample were significantly higher than those in other samples. Content of total anthocyanins of Xidang-A was also about 10~17 mg/L higher than that in Xidang-B. The contents of total phenols, total tannins, esters, aldehyde ketone and terpenes in Xidang-B-X were higher than those in the Xidang-B-Y. Higher altitude was beneficial to improve the color stability of wine and the characteristics of aroma content. Quality of Xidang-A was better than that of Xidang-B, and the brewing process of X was better than that of Y.
    30  Effects of Infrared Drying Technology on Drying Characteristics of Purple Cabbage
    ZHANG Lei YU Xiao-jie BAI Jun-wen XU Pei MA Hai-le ZHOU Cun-shan
    2017, 33(12):202-209. DOI: 10.13982/j.mfst.1673-9078.2017.12.030
    [Abstract](740) [HTML](221) [PDF 757.87 K](1011)
    Abstract:
    The shelf life of purple cabbage could be prolonged by using drying technology. The infrared drying technology had a stronger penetrating capacity, which was suitable for drying of leaf vegetables. Effects of different drying temperature (40 ℃, 50 ℃, 60 ℃, 70 ℃, 80 ℃), infrared radiation distance (5 cm, 11 cm, 16 cm), infrared radiation power (225 W, 450 W, 675 W, 900 W, 1125 W) and air speed (10 m/s, 20 m/s, 26 m/s) on the drying rate, rehydration rate and browning degree of purple cabbage were investigated in this study. Results showed that the drying rate of purple cabbage increased with the increase of temperature, and the radiation distance, infrared radiation power and air speed had little effects on the drying characteristics of purple cabbage. The rehydration rate of samples was greatly affected by drying temperature and infrared radiation power and increased with the increase of temperature, and the highest peak of rehydration rate appeared at 675 W. The drying conditions, based on temperature and energy consumption, were as follows: temperature, 60oC, radiation distance, 11 cm, radiation power, 675 W, air speed, 20 m/s. This research provided reference for the widespread application of infrared drying technology.
    31  Correlation Study on Rice Chalkiness Degree and Steamed Rice Cooking Quality
    TU Xiao-li LI Ji PAN Si-yi WANG Lu-feng
    2017, 33(12):210-215. DOI: 10.13982/j.mfst.1673-9078.2017.12.031
    [Abstract](1097) [HTML](302) [PDF 427.11 K](1336)
    Abstract:
    The cooking quality of five different chalkiness degrees of raw material rice under high-pressure cooking conditions was studied in this paper, and the correlation between chalkiness degree and various indexes of cooking quality was analyzed. The technological conditions of microwave cooking rice were designed for the quality analysis of small amount of rice and the effect of different chalkiness area on gelatinization degree of steamed rice with the microwave cooking technology was investigated. The results showed that the expansion and water absorption of steamed rice were negatively correlated with the chalkiness degree of raw material rice, but not significant. Correlation analysis of texture characteristics and chalkiness degree showed that chalkiness degree mainly affected the hardness of steamed rice but had insignificant effects on the viscosity, cohesiveness, resilience, elasticity, cohesiveness and chewiness. The chalkiness degree of raw material rice had a highly significant negative correlation with the rice pattern and a negative correlation with the taste. Certain correlation also existed in color, relish and aroma, but not significant. The results of microwave cooking showed that the gelatinization speed of chalk rice samples was faster than that of the non-chalk in 3~15 minutes, and the final gelatinization degree of chalk rice samples was higher than that of the non-chalk.
    32  Effect of Superheated Steam Treatment on Bacteria-Reducing and Quality of Green Wheat Berry
    ZHANG Kang-yi SONG Fan-fan YANG Yan YANG Fan KANG Zhi-min GAI Tao
    2017, 33(12):216-220. DOI: 10.13982/j.mfst.1673-9078.2017.12.032
    [Abstract](720) [HTML](158) [PDF 373.24 K](924)
    Abstract:
    The effect of superheated steam treatment on bacteria-reducing and quality of green wheat berry was studied to guarantee the storage quality.The effects on total number of colonies moisture, chlorophyll and starch were investigated by changing the time and temperature of superheated steam treatment. Results showed that the time and temperature of superheated steam treatment greatly affected the bacteria-reducing and chlorophyll but had little effect on the moisture and starch of green wheat berry. The total number of colonies of green wheat berry decreased with the increase of time and temperature of superheated steam treatment while increased with the storage time prolonging. The total number of colony of the green wheat berry was 2.57 log(CFU/g) after the heat steam treatment at 110 ℃ for 3 min, and the contents of moisture, hlorophyll and starch of green wheat berry were 54.42%, 6.5 mg/100 g, 44.2%, respectively, which made the green wheat berry in good quality. The storage time of green wheat berry could be prolonged for 30 d with a total number of colonies of 4.50 log (CFU/g) after the heat steam treatment , which was still in accordance with the food standards. Consequently, the superheated steam could effectively reduce microorganisms in green wheat berry and had little effect on the quality.
    33  Processing Technology of Nutritious-Prepared Millet Bun
    DONG Xiao-han ZHOU Qian NIU Jia-hui FU Meng-qi ZHAO Wen-yang ZHAO Wen
    2017, 33(12):221-227. DOI: 10.13982/j.mfst.1673-9078.2017.12.033
    [Abstract](629) [HTML](176) [PDF 457.69 K](882)
    Abstract:
    In order to develop novel potato food and broaden its applications as well as respond to the nation call of staple potato and promote the development of prepared food, potato flour was used to replace a part of millet flour for the study of the effects of various factors on the quality and processing technology of nutritious-prepared millet bun by using sensory evaluation and texture analysis as the evaluation indexes based on single factor experiment and orthogonal experiment. The results showed that four factors including water volume, percentage of potato flour, flour fineness and water temperature had important influence. The optimal conditions were as follows: amount of potato flour-substitution, 10%, formula was potato powder 10.0%, flour fineness, 100 mesh, water content, 65.0%, water temperature, 80 ℃, steaming time, 10 min. The product shape, color, skin, taste, flavor, sensory quality and repeatability were good under the condition of the optimum formula with a sensory score of 85. The indexes of physical properties were as follows: hardness, 33.82, recovery, 0.10, adhesion, 1.61, cohesion, 0.24, elasticity, 2.23, chewing, 11.81.
    34  Discrimination on Maturity of Plums Based on Hyperspectral Imaging Information
    LI Li-li WANG Bin ZHANG Xue-hao ZHANG Shu-juan
    2017, 33(12):228-232. DOI: 10.13982/j.mfst.1673-9078.2017.12.034
    [Abstract](975) [HTML](207) [PDF 577.08 K](941)
    Abstract:
    In this paper, plum was regarded as the research object, and image informations of different mature degrees of plum fruit (unripe, ripe, mature and overmature) samples were collected based on hyperspectral image. Then the images were treated with median filtering denoising. RGB and HSV color image models were obtained from different mature degrees by Matlab software and average and standard deviation of different components were regarded as the color feature value. RGB, HSV and RGB-HSV color characteristic value were established to identify the plum maturity PLS, and the established models were predicted. The results showed that the accuracy rate of discriminated model based on RGB-HSV color feature value was better than that of RGB and HSV. The accuracy rate of immaturity, instrumental, mature and overmature plum reached 98.35, 90.00%, 85.85% and 90.85%, respectively. The results showed that this discriminated model not only simplified but also enhances the discriminated ability of models, and provided the theoretical basis for the nondestructive detection and discrimination of plum maturity.
    35  Determination of Progesterone in Beef Sample by Gas Chromatography/Combustion/Isotope Ratio Mass Spectrometry
    ZHAO Chao-min GU Shu-qing ZHENG Jiang DENG Xiao-jun YUE Zhen-feng LAI Fu-rao MIN Tian LIU Shao-jie
    2017, 33(12):233-238. DOI: 10.13982/j.mfst.1673-9078.2017.12.035
    [Abstract](654) [HTML](151) [PDF 510.18 K](801)
    Abstract:
    The method of gas chromatography/combustion/isotope ratio mass spectrometry was developed for the determination of progesterone in beef samples in this study. The beef samples were extracted by acetonitrile oscillation and ultrasonic-assisted extraction, and were dehydrated by NaCl to induce separation of water and organic layers. After centrifugation and evaporation, the extracts were further treated with ZnCl2 to remove lipids and purified by solid phase extraction using LC-C18, LC-Si and LC-NH2 cartridges. After Pre-HPLC purification using a C18 column, the samples were analyzed for progesterone by GC/C/IRMS system. The δ13C value of endogenous progesterone in beef was about -25.70±0.13‰ (n=6), and the δ13C value of exogenous progesterone spiked in the sample was about -30.64±0.24‰ (n=6). One-way analysis of variance(ANOVA, p=2.23×10-14<0.05) indicated that there was a highly significant difference between the endogenous and exogenous δ13C value of progesterone, while there was no significant difference between the exogenous and standard solution δ13C value of progesterone. Meanwhile, the simulation experiment showed that the δ13C value of mixture (endogenous and exogenous) of progesterone in actual sample was homologous with the exogenous δ13C value of progesterone. The results showed that the method was specificity and reliable. GC/C/IRMS can be a powerful tool to investigate the origin of natural hormones. The method will fill the domestic technical gap for identifying the origin of hormones.
    36  Detection of Alicyclobacillus spp. Contamination in 6 Commercial Beverages by Electronic Nose
    ZHONG Wu LI Er-hu GUO Xiao-li GUO Xiao WANG Mei-yu ZHANG Yu-sha
    2017, 33(12):239-248. DOI: 10.13982/j.mfst.1673-9078.2017.12.036
    [Abstract](706) [HTML](138) [PDF 745.94 K](937)
    Abstract:
    Alicyclobacillus has the character of high tolerance to acid and heat and the ability of producing spore, which can germinate and propagate in the fruit juice and then make the products smell bad and cause corruption. In this study, the isolated strain XC-6 and standard strain DSM 3922 obtained from the German bacterial collection center were inoculated equally into 6 kinds of fruit juices, respectively. FOX 4000 electronic nose combined with principal component analysis was used for the analysis of inoculated samples and normal samples. The results showed that the electronic nose could identify the contaminated samples quickly and effectively from 6 kinds of fruit juices and’ the cumulative variance contribution rates of the first principal component were more than 95%. GC-MS analysis showed that the prints of volatile compounds between inoculated samples and normal samples were different. The difference of some typical substances content could be used to identify contaminated samples and normal samples by electronic nose. Adjacent ethoxy phenol and guaiacol, the characteristic metabolites of Alicyclobacillus, were detected in the peach beverage and fruit milk mixed drinks, of which the content was significantly higher than the normal samples. The experiments showed that the electronic nose could identify the contaminated samples accurately according to the difference of the juice samples smell maps, which could be used as a testing tool for early detection of Alicyclobacillus pollution.
    37  Building and Analysis of Hyperspectral Detection Models of Bacillus Cereus in Milk
    ZHAO Zi-zhu WEI Yong CHANG Ruo-kui WU Hai-yun LIU Hua SHAN Hui-yong YANG Ren-jie
    2017, 33(12):249-254. DOI: 10.13982/j.mfst.1673-9078.2017.12.037
    [Abstract](784) [HTML](163) [PDF 549.14 K](900)
    Abstract:
    In this paper, a new quantitative detection method based on hyperspectral imaging technology was proposed and studied, which was applied to test Bacillus Cereus in liquid milk. The feasibility of the prediction models for detection the content of Bacillus Cereus in milk was explored by image processing technology, spectral analysis technology and chemical metrology technology, based on the detection of the contamination degree of Bacillus cereus in milk. The image processing technology was used to select the sample area, and the energy value (Energy) texture feature was applied to reduce the dimensions of hyperspectral data to obtain the characteristic value based on the texture feature analysis. A PLS model was built to predict the content of Bacillus Cereus in milk. The correlation coefficients between the calibration set and the prediction set in the PLS prediction model were 0.92 and 0.91, and RMSEC and RESEP were 0.73 and 0.81, respectively. The results showed that the PLS prediction model could only identified the high and low concentration of Bacillus Cereus in the milk. Therefore, the two-dimensional correlation technology combined with N-PLS method was proposed, and the N-PLS prediction model was built. The correlation coefficients between the calibration set and the prediction set in the N-PLS prediction model were 0.99 and 0.99 respectively, and RMSEC and RESEP were 0.02 and 0.09, respectively. The results showed that the N-PLS model had higher accuracy and was able to achieve quantitative analysis of Bacillus Cereus in milk.
    38  Effects of Different Processing Methods on the Flavor and Quality of Korla Pear Juice Evaluated by GC-MS Combined With PCA
    ZHANG Fang ZHAN Ping ZHOU Wen-jie WANG Peng TIAN Hong-lei
    2017, 33(12):255-261. DOI: 10.13982/j.mfst.1673-9078.2017.12.038
    [Abstract](1878) [HTML](268) [PDF 493.18 K](878)
    Abstract:
    In order to explore the effects of different processing methods on the volatile substances and the sensory attributes of Korla pear juice, the volatile components of Korla pear juices under different processing methods were isolated and identified, and the correlation analysis between the volatile components and the sensory attributes were analyzed by static headspace gas chromatography-mass spectrometry (HS-GC-MS) technology combined with principal component analysis (PCA) technique. The results showed that pectinase enzymolysis and pasteurization had a significant effect on the volatile substances and the sensory attributes of pear juice (p<0.05), in which the esters were lost or significantly reduced, while alcohols, hydrocarbons and heterocycles significantly increased. Pectinase enzymolysis and pasteurization reduced the overall sensory quality of Korla pear juice and pasteurization made the peculiar smell and cooking smell of Korla pear juice significantly abnormal. The PCA analysis showed that there was a good positive correlation between ethyl acetate, ethyl caproate, ethyl propionate, ethyl butyrate, hexyl alcohol as well as nonanal and fruity fragrant sensory properties of Korla pear juice. There is a good positive correlation between hexyl acetate and flower fragrant sensory properties of Korla pear juice.
    39  Determination of Imidacloprid Residues and Pyrimethanil Residues in Green Vegetable (Brassica rapa var. chinensis) by Ultra Performance
    QIN Guo-xin LAO Shui-bing MO Ren-fu YAN Fei-yan MO Lei-xing HE Jie ZHOU Qi-feng YANG Yu-xia LUO Li-hong
    2017, 33(12):262-266. DOI: 10.13982/j.mfst.1673-9078.2017.12.039
    [Abstract](684) [HTML](154) [PDF 498.74 K](901)
    Abstract:
    A method for simultaneous determination of imidacloprid residues and pyrimethanil residues in green vegetable (Brassica rapa var. chinensis) by using Ultra performance liquid Chromatography-Tandem Mass Spectrometry (UPLC/MS/MS) was established in this paper. The green vegetable (Brassica rapa var. chinensis) samples were extracted by acetonitrile and purified by an amino solid phase extraction (SPE) column (eluted by acetonitrile + toluene (3+1)), and then the gradient elution was applied in ultra performance liquid chromatography separation by using the acetonitrile -0.1% formic acid solution as mobile phase. Samples were detected by mass spectrometry multi reaction monitoring model, using retention time and sub-ion ratio as qualitative analysis and the external standard method as quantitative analysis. Tandem mass spectrometry with electrospray ionization and multiple reaction monitoring interface in an external standard manner. The results showed that the limits of quantitation (LOQ) of imidacloprid and pyrimethanil were 3.1×10-6 mg/kg and 3.6×10-7 mg/kg respectively, and the correlation coefficients of two pesticides were more than 0.9995 in the linear range of 0.0005~0.5 mg/L. In addition, the average recoveries were 99.1 %~105.8 % for imidacloprid and 99.1 %~110.0 % for pyrimethanil in the range of 0.02-0.2 mg / kg, and the relative standard deviations (RSD) were less than 1.23 % and 1.64 %, respectively. The proposed method had the advantages of simple operation, wide linear range, high sensitivity and reliabilty, which met the requirements for the detection of such drug residues in vegetables at home and abroad.
    40  Nondestructive Detection of Moisture Content in Fresh Mutton Using near Infrared Spectrometry of Southern Xinjiang
    CHEN Jie YAO Na
    2017, 33(12):267-271. DOI: 10.13982/j.mfst.1673-9078.2017.12.040
    [Abstract](672) [HTML](199) [PDF 395.01 K](749)
    Abstract:
    In order to realize fast nondestructive testing of moisture content fresh mutton in southern Xinjiang, the spectroscopic data of the 134 samples of same variety in fresh hind legs meat of sheep in the range of 780~1700 nm wavelength were collected in this study. The original spectrums were processed by noise reduction such as median smoothing filtering, multiple scatter correction, a derivative, standardization, centralized transformation, smoothing retreatment methods, and S-G smoothing. The samples were divided into training set and test set by a ratio of 13:1. PLSR was adopted to establish the prediction model to forecast the moisture content of the fresh mutton. The results showed that the prediction correlation coefficient of training set was 0.94, its MSEC was 0.04, and prediction success rate was 97.6%, while the prediction correlation coefficient of the test set was 0.89, its standard deviation MSEV was 0.89, and the success rate of prediction was 96.4%. Experimental results confirmed that the established PLSR model combined with preprocessing such as median smoothing filtering, multiple scatter correction, a derivative, standardization, centralized transformation, and the S-G smooth could provide accurately fast nondestructive evaluation for the moisture content of fresh mutton, and provide theoretical reference application for rapid nondestructive testing of the moisture content in fresh mutton.
    41  Advances on Yeast Biological Detoxification
    HU Xiao-qing CHU Zhao-juan ZHANG Ji-xiang LIU Shuai YI Yong SUN Chun-yang WANG Dong
    2017, 33(12):272-277. DOI: 10.13982/j.mfst.1673-9078.2017.12.041
    [Abstract](904) [HTML](336) [PDF 427.94 K](1224)
    Abstract:
    Yeast is a large class of unicellular eukaryotic microorganism and widely used in food brewing and industrial biotechnology for the production of ethanol, protein, and organic acid. Recent studies have revealed that yeast also has the function biological detoxification and the application prospects in the field of environmental protection, food and feed. Based on this, the review summarized the latest research advances on yeast biological detoxification. The harmfulness of common mycotoxins in food and feed and the detoxification methods were introduced first and then the application of different yeast strains in attenuating or eliminating mycotoxins (aflatoxin, ochratoxin A, patulin, zearalenone, and deoxynivalenol) was summarized. The mechanism of biological detoxification mediated by yeast beta-glucan and glycoprotein was introduced, and the detoxification effect of Saccharomyces cerevisiae and Yarrowia lipolytica on bacterial endotoxin and the possible mechanism were also summarized. Finally, the detoxification effect of yeast on heavy metals and other pollutants and the possible mechanism were introduced, and the research of yeast biological detoxification was prospected.

    Editor in chief:李琳

    Inauguration:现代食品科技

    International standard number:ISSN 1673-9078

    Unified domestic issue:CN 44-1620/TS

    Domestic postal code:46-349

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