Volume 33,Issue 10,2017 Table of Contents

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  • 1  Effects of Purple Sweet Potato Extract on the Lifespan of Caenorhabditis Elegans
    LI Xiang ZHANG Ze-sheng TANG Xin-hui KANG Yi-jun WANG Hui YANG Sheng-nan WANG Hao
    2017, 33(10):1-6. DOI: 10.13982/j.mfst.1673-9078.2017.10.001
    [Abstract](1131) [HTML](276) [PDF 494.67 K](1263)
    Abstract:
    Effects of Purple sweet potato extract (PSPE) on the lifespan of Caenorhabditis elegans (C. elegans) and its possible mechanism were studied. C. elegans were fed in normal culture medium and NGM culture medium containing different concentrations (70, 140, 280 μg/mL) of PSPE to investigate the effects of PSPE on the lifespan of C. elegans as well as the content of lipofuscin, superoxide dismutase (SOD) and catalase (CAT) and the expressions of antioxidant-related genes in vivo. The Results showed that the average lifespan of C. elegans fed with 280 μg/mL PSPE increased by 33%, the accumulation of lipofuscin decreased by 30%, the activity of SOD and CAT increased by 17.7% and 47.5%, respectively, compared with the blank group. The mRNA levels of daf-16, ctl-1 and sod-3 in nematodes were increased in different degrees, and the mRNA expressions of daf-16, ctl-1 and sod-3 in 280 μg/mL PSPE group were increased by 23.2%, 37.4% and 84.6%, respectively; While the mRNA expression of age-1 in 280 μg/mL PSPE group was decreased by 26.4%.
    2  Effects of Mucuna pruriens (Linn) Ethanol Extracts on Glucose and Lipid Metabolism in 3T3-L1 Adipocytes
    SONG Jia-le QIAN Bo GUI Zhong-yu PARK Kun-young ZHOU Yan-yuan
    2017, 33(10):7-12. DOI: 10.13982/j.mfst.1673-9078.2017.10.002
    [Abstract](873) [HTML](169) [PDF 524.41 K](901)
    Abstract:
    To investigate the effect of Mucuna pruriens (Linn) ethanol extracts (MPEE) on glucose and lipid metabolism in insulin resistance 3T3-L1 adipocytes. The insulin resistance 3T3-L1 adipocyte model was induced by DMEM medium containing high concentration of glucose and insulin. The levels of glucose consumption, free fatty acid (FFA), glycerol, cellular triglyceride(TG), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the culture medium were determined by commercial assay kits after the cells treated with different concentrations of MPEE for 24 h. The mRNA expression levels of glucose transporter-4 (GLUT-4), hormone-sensitive triglyceride lipase(HSL) and inflammation related factors [including TNF-α, IL-6, monocyte chemotactic protein-1 (MCP-1) and C-reaction protein (CRP)] in cells were determined by Quantitative real-time PCR (qRT-PCR) assay. MPEE could enhance the glucose consumption, decrease the cellular TG levels and reduce the releasing of FFA and glycerol in insulin resistance cells. MPEE also reduced the secretion of TNF-α and IL-6, and reduced the mRNA expressions of inflammation mediators (including TNF-α, IL-6, MCP-1 and CRP) in model cells. In addition, MPEE was able to up-regulate the mRNA levels of GLUT-4, and reduce the mRNA levels of HSL in insulin resistance cells. These results suggested that the MPEE treatment was able to regulate the glucose and lipid metabolism to against the insulin resistance via increasing the glucose consumption, enhancing the TG lipolysis, and reducing the releasing of FFA and glycerol in 3T3-L1 adipocytes. MPEE treatment also down-regulated the inflammation levels by reducing the secretion of inflammatory mediators.
    3  Analysis of Evolutionary Relationship of GH78 Fungal α-L-Rhamnosidase
    GONG Jian-ye WU Zhe-yu LI Li-jun NI Hui
    2017, 33(10):13-20. DOI: 10.13982/j.mfst.1673-9078.2017.10.003
    [Abstract](1083) [HTML](186) [PDF 745.30 K](1123)
    Abstract:
    In order to analyze the conserved regions and the evolutionary relationship of fungal α-L-rhamnosidase in glucoside hydrolase family 78 (GH78), 87 sequences of GH78 fungal α-L-rhamnosidases were collected and compared by ClustalW2 software. In addition, the conserved amino acid sites and the common conserved regions of GH78 fungal α-L-rhamnosidases were obtained by Block Maker. Meanwhile, the signal peptide was predicted by SignalP 4.1 Server. Besides, the genetic distance and the phylogenetic tree of 87 sequences were constructed by MEGA6.0 software. Although the amino acid sequences of fungal α-L-rhamnosidases had various lengths, all contained three conserved regions, including AHGWSTGPTY, VTLDTGQNVAG and NELSIPTDGAKRD, as well as a number of conserved amino acid sites. Moreover, 29 sequences contained signal peptides. The genetic distance between the genera was from 0.7 to 48.5, and the genetic distance of interspecies was from 0.4 to 48.5. Interestingly, it was found that the phylogenetic tree of the phylogenetic system was not clustered by the source of the strain, but on whether the extracellular enzyme was clustered. In this study, the conserved region and evolutionary relationships of GH78 α-L-rhamnosidases were analyzed, which laid the foundation for engineering modification of α-L-rhamnosidase.
    4  Effects of Electrostatic Field on Ice Crystals Formation of Glucose and Sucrose Solution
    GAO Wen-hong CHEN Qiu-yan WANG Qi-jun SUN Da-wen ZENG Xin-an HAN Zhong
    2017, 33(10):21-29. DOI: 10.13982/j.mfst.1673-9078.2017.10.004
    [Abstract](854) [HTML](253) [PDF 658.34 K](792)
    Abstract:
    The self-designed and electrostatic field-assisted freezing-equipment was used to investigate the effects of electrostatic field on ice nucleation and crystals growth in freezing process using different concentrations (5, 50, 100, 150 g/L) of glucose and sucrose solution. Different electrostatic field intensity (0, 4, 6, 8, 10 kV) was applied during the process of freeze, and super cooling temperature and phase transformation time were used as the evaluation parameters. The results showed that the effects of different electrostatic field intensity on ice growth of glucose and sucrose solution were different, which was concerned with solution concentrations. Higher electrostatic field intensity (6 kV and 8 kV) could promote the formation of ice nucleation for 5 g/L, 50 g/L glucose solution, as well as 5 g/L sucrose solution, and the higher electrostatic field intensity (6 kV) might be favorable to ice nucleation formation for 50 g/L glucose and sucrose solution. Consequently, the electrostatic field had an unfavorable effect on the diffusion of water molecules in glucose and sucrose solution in the process of ice crystals growth.
    5  Effects of Milks on the Distribution of Tea Polyphenols in Nano-aggregates from Green Tea and Black Tea Infusions
    CAI Cui-ling CHEN Zhong-zheng ZHANG Yuan-yuan LI Bin LIN Xiao-rong
    2017, 33(10):30-36. DOI: 10.13982/j.mfst.1673-9078.2017.10.005
    [Abstract](1508) [HTML](176) [PDF 632.66 K](1087)
    Abstract:
    Tea nano-aggregates were colloidal particles spontaneously assembled by tea polyphenols, proteins and other components in natural tea infusions. These peculiar particles were the intermediate states of tea components between free molecules and precipitates. To primarily explore the effects of milks on the distribution of tea polyphenols in tea infusions, tea infusions extracted from green tea and black tea at tea-water ratio of 1:50, and then mixed with whole milk, semi-skimmed milk and skimmed milk of various percentages, respectively. Tea nano-aggregates in these milk teas were subsequently separated by ultrafiltration centrifugal technology. In addition, total intensity and average diameter of tea nano-aggregates were analyzed by dynamic light scattering; meanwhile, the concentration and distribution of tea polyphenols in tea nano-aggregates were evaluated by Folin-Ciocalteu method. The effect of milk addition ratio, fat content and variety of tea on the physical and chemical characteristics of nano-aggregates, as well as the distribution characteristics of tea polyphenols was studied. The results showed that the addition of milk with different fat content would promote the combination of some milk nanometer micelles and tea nano-aggregates, resulting in more than 90% of tea polyphenols in original tea infusions enriched in tea nano-aggregates, and the ratio of free tea polyphenols in green tea and black tea infusions reduced by 78% and 67%, respectively. In conclusion, adding milks to green tea and black tea infusions would strengthen the aggregation of tea polyphenols and remarkably reduce the ratio of free tea polyphenols.
    6  Analysis of Antibiotic Resistance and Related Genes of Salmonella enteritidis and Salmonella havana
    WU Yun WU Hao-tian SONG Sheng-jian YIN Ming-yuan TIAN Ge MA Wen-rui WANG Wei ZHANG Ya-nan GULINAZI
    2017, 33(10):37-44. DOI: 10.13982/j.mfst.1673-9078.2017.10.006
    [Abstract](1163) [HTML](182) [PDF 533.90 K](852)
    Abstract:
    The drug susceptibility and the related resistance genes of 17 strains of salmonella enteritidis and 11 strains of salmonella Havana had been studied in detection by Xinjiang Urumqi part of the farmer's market, in order to ensure food safety, better understanding the development of drug resistance and the ways to spread. The drug sensitivity of Salmonella was evaluated by agar dilution method. In addition, PCR and gene sequencing were used to detect the presence of mutations in the quinolone resistancedetermining region (QRDR) and plasmid-mediated quinolone resistance genes. The susceptibility rates of these 17 Salmonella enteritidis isolates were 100% to ciprofloxacin and cefoxitin, these Salmonella isolates were 94.1%, these nalidixic acid and ceftriaxone isolates were 17.6%, and the gene detection rate of qnrB was 64.7%, 17 strains of Salmonella enteritidis GyrA gene mutation, the main mutation type Asp87Tyr; The drug resistance rates of 11 Salmonella havana isolates totrimethoprim, chloromycetin, sulfadimethisoxazol, sulfamethoxazole/trimethoprim, nalidixic acid, cefoxitin were 100%, 63.6%, 36.4%, 18.2%, 9.1%, 9.1% and the sensitive rate of ampicillin, amoxicillin/clavulanic acid was 100%, the detection rate of qnrB, qnrS is 9.1%, gene mutation type of 11 strains of Salmonella Havana ParC is Thr57Ser. Salmonella enteritidis and Salmonella havana drug resistance is serious in Xinjiang Urumqi. There should be concerns about the serious case of resistance to antimicrobial in Salmonella.In addition, the quinolone resistancedetermining region (QRDR) mutations and the plasmid-mediated quinolone resistance genes may affect the antimicrobial resistance of Salmonella enteritidis and Salmonella havana.
    7  Extraction of Polysaccharides from Gracilaria lemaneiformis and Their Immunoregulatory Activity
    REN Ying-lang JI De-sheng ZHANG Yun-lin WEN Ling-rong YOU Li-jun
    2017, 33(10):45-51. DOI: 10.13982/j.mfst.1673-9078.2017.10.007
    [Abstract](930) [HTML](195) [PDF 591.91 K](975)
    Abstract:
    The polysaccharides from Gracilaria lemaneiformis were exacted using hot water, different acids, and enzymes. The effects of different methods on the extraction rate were compared, and the chemical composition and immunoregulatory activity of polysaccharides extracted using acids were evaluated. The results showed that the yield of the polysaccharides extracted using citric acid was the highest (27.66 ± 0.49%), which was 1.86 times those extracted using hot water. The amounts of total sugar, proteins, uronic acids, and sulfates of the polysaccharides exacted using citric acid were 62.24 ± 1.09%, 1.54 ± 0.15%, 6.25 ± 0.72%, and 16.74 ± 0.11%, respectively. No polyphenols were detected. In addition, the polysaccharides extracted using citric acid significantly promoted the proliferation of RAW 264.7 cells, and enhanced their pinocytic capability. G. lemaneiformis polysaccharide improved the thymus and spleen indices in photoaged mice, as well as reduced the concentration of interleukin-10 and increased tumor necrosis factor-α content in mouse serum. These findings suggest that G. lemaneiformis polysaccharides have a relatively high capacity to enhance the immune functions of mice and can alleviate immunosuppression during the photoaging process.
    8  Isolation and Identification of Chemical Constituents and In Vitro Antioxidant Activity of Ethyl Acetate Extracts from Psidium guajava Linn Leaves
    FU Chun-li PENG Yan LI Shi-min XIAO Sheng-hong CHEN Hua-xu
    2017, 33(10):52-57. DOI: 10.13982/j.mfst.1673-9078.2017.10.008
    [Abstract](941) [HTML](204) [PDF 461.02 K](1100)
    Abstract:
    The in vitro antioxidant activity and the material basis for its antioxidant activity of the ethyl acetate extracts from Psidium guajava leaves were investigated in this study. The antioxidant ability of ethyl acetate extracts was first analyzed in vitro after 95% ethanol percolating, n-hexane and ethyl acetate extraction from Psidium guajava leaves, and then the chemical compounds were isolated and identified by combing column chromatography (such as silica gel column chromatography, Sephadex LH-20) and spectrometry (such as mass spectrometry, nuclear magnetic resonance spectroscopy). The results showed that the ethyl acetate extracts had strong antioxidant activity in vitro to scavenge DPPH radicals, superoxide anion free radicals, and hydroxyl radicals in a dose-dependent manner, and when the concentration was 1 mg/mL, the scavenging abilities for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, superoxide radicals and hydroxyl radical were 94.15%, 87.88%, 67.3%, respectively, which were close to or better than those of Vc. Five compounds were isolated and purified from the ethyl acetate extracts of Psidium guajava leaves by silica gel column chromatography and Sephadex LH-20 column chromatography, four of which were identified as demethoxymatteucinol (1), cryptostrobin (2), quercetin (3), and isoquercitri (4), respectively, by comparison of their mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopic data with those in literatures. Furthermore, compound 2 was the first flavanone isolated from the genus Psidium Linn.
    9  Inhibitory Activity of Trans-cinnamaldehyde Against Cronobacter sakazakii
    SHI Chao GUO Du ZHANG Wen-ting GUO Kai-lun WEN Qi-wu GUO-Xiao LIU Zhi-yuan YIN Shu-hua YANG Bao-wei XIA Xiao-dong
    2017, 33(10):58-66. DOI: 10.13982/j.mfst.1673-9078.2017.10.009
    [Abstract](924) [HTML](160) [PDF 665.36 K](1143)
    Abstract:
    In order to find a safe and effective control method for Cronobacter sakazakii (C. sakazakii), the antimicrobial activity of trans-cinnamaldehyde (TC) against C. sakazakii and its possible mechanism of action were investigated in this study. The minimum inhibitory concentrations (MICs) of TC against C. sakazakii and its effect on growth curve of C. sakazakii were determined. The effects of TC on cell membrane integrity and cell morphology of C. sakazakii were assessed by using LIVE/DEAD BacLight bacterial activity assay kit, laser scanning confocal microscope and field emission scanning electron microscope. The inhibitory effect of TC on C. sakazakii at 4 ℃, 25 ℃ and 37 ℃ was studied by constructing a reconstituted infant formula model (containing 0%, 0.1%, 0.2% or 0.4% TC), and the practical effect of TC was fully considered. The results showed that the MICs of TC against C. sakazakii ranged from 0.4 to 1.0 mg/mL and TC could effectively prolong the growth lag phase of C. sakazakii. Compared with control group, the membrane integrity of the C. sakazakii strain treated with TC at MIC and 2 MIC wasdramatically declined to 60% and 46%, respectively. The field emission scanning electron microscope confirmed that the samples treated with TC had more wrinkled surface compared with the smooth surface of the untreated cells TC at 0.4% (V/V) reduced the number of C. sakazakii in the reconstituted infant formula below the detection limit at 25 ℃ for 90 min or at 37 ℃ for 60 min. In conclusion, TC had the potential to be used as a natural antimicrobial substance in foods such as milk powder and could be further used in the food to effectively prevent and reduce related foodborne diseases caused by C. sakazakii.
    10  pH Effect on Degradation of β-ethylene Glycol Phenyl Ether Catalyzed by Sulfur Compounds in Micelle Media
    LI Xiao-hong ZHANG Yi CHEN Xin WU De-zhi
    2017, 33(10):67-71. DOI: 10.13982/j.mfst.1673-9078.2017.10.010
    [Abstract](658) [HTML](232) [PDF 532.93 K](792)
    Abstract:
    Three sulfur-containing nucleophiles with different pH, five different kinds of micelle media and mercaptoethanol in two solvents were used to catalyze the degradation of β-ethylene glycol (EGPE) in this study. The results showed that the reaction rate was much greater in 1,4-dioxane than that in ethanol, reaction rate increased with the increase of volume of 1,4-dioxane and ethanol and then reached to the maximum when the volume of 1, 4-dioxane and ethanol increased to 14 mL (ratio of 1,4-dioxane with ethanol and water was 7:3), and then decreased with the volume exceeded 14 mL. Meanwhile, the change of pH value had an effect on the degradation rate in five different kinds of micelle media, and the reaction rate increased rapidly with the increase of pH and then decreased when the pH value exceeded pKa. In conclusion, polarity of solvent, dielectric constant and pH value had an effect on the catalytic activity of nucleophiles.
    11  Does-effect Relationship of Active Ingredients from Supernatant Water of Dendrobium devonianum Paxt.
    ZHANG Yue WANG Dong-hui FANG Fang WANG Yan XING Cen-can WANG Feng-zhong
    2017, 33(10):72-76. DOI: 10.13982/j.mfst.1673-9078.2017.10.011
    [Abstract](1042) [HTML](204) [PDF 533.35 K](1059)
    Abstract:
    In order to utilize the resource of Dendrobium devonianu Paxt., the content of alkaloids, polyphenols, flavonoids and polysaccharides in the supernatant of Dendrobium byproducts were determined after the polysaccharides were extracted by traditional method and enzyme assisted extraction method. The antioxidant and hypoglycemic activities of the supernatant were characterized by the DPPH free radical scavenging rate, the hydroxyl radical scavenging rate, the total reducing capacity and α-amylase inhibition capacity. The results showed that alkaloids, polyphenols and flavonoids and polysaccharides content as well as biological activity in supernatant of Dendrobium byproducts after the polysaccharides were by pectinase assisted extraction were much higher than those in traditional method. The content of alkaloid, polyphenols, flavonoids and polysaccharide in supernatant of Dendrobium, treated with 7.5 U pectinase has the highest material content were 0.1971, 0.6873, 28.4598 and 7.0500 mg/g, respectively; The supernatant of Dendrobium devonianu Paxt treated with 12.5 U pectinase have the strongest antioxidant capacity, the DPPH free radicals’ and hydroxyl lradicals IC50 value were1.25 and 4.1 mg/mL, respectively. After analysis the relationship of does-effect, the content of polyphenols, flavonoids in the supernatant and the total water extract extracted by the traditional method were significantly correlated with the functional indexes, there was no significant correlation between the supernatant and the functional indexes after enzyme treatment.
    12  Antibacterial Activity of Volatile Oil and Residue Extract from Lonicera Japonica
    RUAN Zhi-yang WANG Zhao-mei LI Jia-jia WEI Xi-qiong
    2017, 33(10):77-82. DOI: 10.13982/j.mfst.1673-9078.2017.10.012
    [Abstract](968) [HTML](192) [PDF 475.37 K](1000)
    Abstract:
    Lonicera japonica, obtained from Shandong and Guangdong, was used as the raw material in this study. The volatile oil was separated by CO2 supercritical fluid extraction, and afterwards the water-soluble and the water-insoluble subfraction were prepared from the residue after volatile oil isolation. The chemical composition of volatile oil was analyzed by GC-MS and high performance liquid chromatography was used to determine the content of chlorogenic acid and galuteolin in residue extract of Lonicera japonica. The anti-bacterial activities of volatile oil and extract were investigated by disk diffusion assay and minimum inhibitory concentration (MIC). The main components of Lonicera japonica volatile oil were fumaronitrile (61.5%) and amyl acetate (37.7%), and the content of lorogenic acid and galuteolin in water-soluble residue extract from Shandong province was the highest among the 4 residue extracts. The Lonicera japonica volatile oil and subfractions of ethanol extract exhibited different inhibitory effects on the six pathogens. The antimicrobial effect of the two extracts on gram-positive bacteria was better than that of gram-negative bacteria. The antibacterial effect of the volatile oil in Lonicera japonica from Shandong was the strongest with the MIC of 64 μL/mL and the diameter of inhibition zone of 9.3 mm. Meanwhile, the water-soluble subfraction of Lonicera japonica from Guangdong showed a significantly high antibacterial effect on bacillus subtilis with the diameter of inhibition zones above 15mm and MIC value of 16 μL/mL. There was no significant difference in the antibacterial activity of the extract in Lonicera japonica from Guangdong and Shandong.
    13  Mathematical Modeling for Vacuum Drying Characteristics of Peach Pomace
    LI Cong ZHOU Mo BI Jin-feng CHANG Xue-dong CHEN Qin-qin NING Chun-yuan
    2017, 33(10):83-88. DOI: 10.13982/j.mfst.1673-9078.2017.10.013
    [Abstract](660) [HTML](238) [PDF 530.34 K](913)
    Abstract:
    The drying characteristics, effective moisture diffusivity (Deff) and activation energy (Ea) of peach pomace with vacuum drying at 55 ℃, 65 ℃, 70 ℃ and 80 ℃ were investigated in this study. The experimental data obtained were fitted to ten thin-layer drying models. The results indicated that drying temperature had a significant influence on the drying rate of peach pomace. The drying time decreased with the increasing drying temperature, and the drying process appeared in the falling rate periods. Compared the results of ten thin-layer drying models, models of Wang and singh were the best to describe the drying process of peach pomace under all drying conditions (R2 > 0.99776), and there was a good agreement between the experimental and predicted values. According to the Fick’s second law, the values of effective moisture diffusivity varied from 8.0855×10-10~1.5340×10-9 m2/s. The relationship between the effective moisture diffusivity and drying temperature could be described by Arrhenius-type relationship, which resulted in activation energy of 21.1 kJ/mol for peach pomace. This study provides a theoretical basis for the application of vacuum drying technology in the drying of peach pomace.
    14  Chemical Composition, DPPH Free Radical Scavenging and Antimicrobial Activity of the Essential Oil and Six Compounds Isolated from Spiraea mongolica Maxim
    ZHANG Wen-huan QIAN Han SONG Ya-jie SHEN Tong
    2017, 33(10):89-95. DOI: 10.13982/j.mfst.1673-9078.2017.10.014
    [Abstract](761) [HTML](174) [PDF 378.98 K](744)
    Abstract:
    The essential oil was extracted from the dried twigs and leaves of Spiraea mongolica Maxim by hydro-distillation and identified by GC-MS. The main compositions of the oil were fatty acids and their derivatives, ethyl palmitate (38.631%), ethyl linolelaidate (23.576%) and ethyl linolenate (14.634%). From the dried twigs and leaves, botulin (1), lupine-3,20-diol (2), 1β-hydroxyl-6,9-dien-8-oxoeremophil -11-nor-11-ketone (3), 3-(4-Methoxyphenyl) propanal (4), stigmasterol (5), β-Sitosterol (6) were isolated by silica gel column chromatography and identified by NMR data. Their DPPH free radical scavenging and antimicrobial activities were separately evaluated. The essential oil showed good to moderate DPPH free radical scavenging activity (IC50 = 900 μg/mL), while had no apparent antimicrobial activity. Compound (4) exhibited reasonably strong DPPH free radical scavenging ability (IC50 = 13 μg/mL) and showed diverse antimicrobial competence against Aspergillus flavus, Bacillus subtilis and Candida albicans. Compound (3) showed antimicrobial activity against Aspergillus flavus, Bacillus subtilis and weak DPPH free radical scavenging activity. These results of the study had certain significance in the study of traditional Chinese medicine plant and development of new drugs.
    15  Predictive Modeling for Monitoring the Quality Change and Shelf-life of Frozen Tilapia Fillets in Cold-chain
    JIANG Yuan-xin YE Wen-jie JIANG Hong-rui ZHAO Mou-ming LIU Xiao-ling
    2017, 33(10):96-104. DOI: 10.13982/j.mfst.1673-9078.2017.10.015
    [Abstract](874) [HTML](211) [PDF 751.30 K](798)
    Abstract:
    This study examined the quality change as well as the sensitive index of frozen tilapia fillets stored at -18 ℃, -14 ℃, -10 ℃, according to the results of sensory, microorganism and physicochemical index. The results showed that the total plate count of numerical values rising slowly with the prolongation of storage time, and reached to 4.31 log(CFU/g), 4.76 log(CFU/g), 5.47 log(CFU/g), respectively at 120 days when stored at -18℃, -14℃, -10℃. It did not reach the national limit of the total number of frozen tilapia fillets. Total volatile basic nitrogen (TVB-N) values rose linearly and reduced to 13 mg/100 g at 120 d, 80 d, 60 d, respectively. Thiobarbituric acid (TBA) value increased gradually but there were no significant differences amongst groups. The sensory quality of frozen tilapia fillets gradually decayed with storage time, but maintained a good sensory quality at the end of storage, and the lower the temperature of freezing storage had, the slower the quality decayed. According to Pearson’s correlation coefficient, TVB-N was the sensitive index and its change was accordance with zero order reaction when combining with the Goodness-of-fit analysis and Residual sum of squares (RSS). Using Arrhenius equation to build dynamic equation between storage temperature (T) and the reaction rate (ka), the shelf-life prediction model of frozen tilapia fillets in cold-chain logistics was built. It has proved that this model can accurately predict the shelf life of frozen tilapia fillets at -14 ℃ and -10 ℃.
    16  Mass Transfer Model of Citronellal Separated from Citronella Oil by Short Path Distillation
    ZHANG Feng-zhen ZHANG Wei LIU Xing-yong YANG Hu MA Xie
    2017, 33(10):105-109. DOI: 10.13982/j.mfst.1673-9078.2017.10.016
    [Abstract](861) [HTML](192) [PDF 546.79 K](812)
    Abstract:
    The short path distillation has been widely used in purification and refining of food additives for its advantages of short heating time, high separation efficiency and preservation of the natural actives. Accurate prediction of separation purity and separation efficiency play an important role in parameters optimization and experiment design of short path distillation process. The citronellal was separated from citronella oil by short path distillation in this work and the corresponding multicomponent mass transfer model, which simulated the separation process, was established based on Langmuir-Kundsen equation. The effects of different process conditions on the purity and separation efficiency of citronellal were predicted, and the simulation results were consistent with the experimental data. The results showed that the separation of citronellal was mainly processed in the front, nearly middle part of the evaporator (z/L = 0.5), with a ultimate separation efficiency of 60%. Increasing feeding rate, decreasing evaporating temperature or evaporation area was beneficial for the purification of citronellal, while reduced the separation efficiency. Based on the results of purity and separation efficiency of citronellal, the optimum process conditions were as follows: feeding rate, 4~5 mL/min; temperature, 65~70 ℃.
    17  Purification, Solid-phase Synthesis and Structural Differentiation of Casein Phosphopeptides Active Monomers
    LIU Guo SUN Sheng-wei MIAO Jian-yin LUO Zhen XIA Zu-meng LIU Fei GUO Bao-yan CAO Yong
    2017, 33(10):110-116. DOI: 10.13982/j.mfst.1673-9078.2017.10.017
    [Abstract](1155) [HTML](226) [PDF 536.94 K](968)
    Abstract:
    In this paper, the preliminary purification of casein phosphopeptides(CPPs) was investigated using preparative reverse phase high performance liquid chromatography. Four components were collected according to the peak shape of the chromatogram. The F2 component was further separated and purified by analytical RP-HPLC to obtain sub-component P3 The casein phosphopeptides active monomers P3 powder was collected by reduced pressure distillation and lyophilization to remove the solvent. The casein phosphopeptide monomers P3 were synthetically synthesized based on the amino acid sequence of P3. The molecular weight of synthesized P3 was uniform with the natural monomer and the puritywas 98.13%, which was determined by Mass Spectrometry and High Performance Liquid Chromatography. The retention time of the natural and synthetic casein phosphopeptides monomer P3 was consistent, and the ultraviolet absorption of the synthesized monomer was much higher than that of the natural monomer, which could be related to the diversity of protein tertiary structure. There were α-helix, β-turn, β-sheet and random coil in the secondary structure of the natural and synthetic CPPs monomer P3 according to the analysis of Fourier Transform Infrared Spectroscopy (FT-IR) and circular dichroism. The synthesized and natural monomers were mainly in the form of antiparallel and random coil, but there were difference in the ratio of the α-helix, β-turn and random curl of natural and synthetic CPPs monomer P3. The second structural change of natural monomer P3 changed small with the concentration of monomers from 2 mg/mL up to 4 mg/mL, which the α-helix and random coil were transformed to the antiparallel. The second structural of synthesized monomer P3 had a significant variation, which mainly transformed the α-helix and β-turn to the antiparallel and random coil.
    18  Optimization of Extraction Technology of Total Flavonoids and Puerarin in Rdix Puerariae and their Enhanced Antioxidant Capacity in Colitis Rats
    WU Yi LIU Wen-hui HUANG Fang YAN Ya-li OUYANG Ke-xian LIU Jian-ping ZHOU Shi-cheng RUAN Zheng
    2017, 33(10):117-126. DOI: 10.13982/j.mfst.1673-9078.2017.10.018
    [Abstract](861) [HTML](181) [PDF 742.58 K](928)
    Abstract:
    Based on the traditional ethanol heat reflux method to extract puerarin and flavone, the quadratic-orthogonal-rotation -combination design was used to optimize the extraction of total flavonoids and puerarin from Rdix Puerariae. Regression models were established for the yield of total flavonoid (y1) and the yield of puerarin (y2) with the other four test factors (including extraction temperature (x1), reflux time (x2), ethanol concentration (x3) and ratio of material to liquid (x4), respectively. The regression model showed that, the most important two factors that affected the total flavonoids yield and puerarin yield from Radix Puerariae were: extraction temperature (x1) and ethanol concentration (x3). The optimal technical conditions were: extracting at 85 ℃ for 55 min, reflux time 55 min, ethanol concentration of 30%, solid-to-liquid ratio of 1:17.5. Under these conditions, the extraction rate of total flavone from Radix Puerariae was 41.07 mg/g, the extraction of puerarin rate could be 16.50 mg/g, and the optimized extraction technology of Radix Puerariae was stable and feasible. The results showed that the Rdix Puerariae extractive had a better effective in recovering serum catalase (CAT), Cu/Zn superoxide dismutase (SOD), and phospholipid hydroperoxide glutathione peroxidase (GSH-px) levels as well as antioxidant capacity in colitis rats as compared with pure puerarin.
    19  Separation and Antioxidant Activity Evaluation of Melanoidins from Lager Beer
    ZHENG Jin-de SUN Mei-xia YAO Ji-wei ZHAO Hai-feng
    2017, 33(10):127-132. DOI: 10.13982/j.mfst.1673-9078.2017.10.019
    [Abstract](1064) [HTML](231) [PDF 467.14 K](930)
    Abstract:
    The antioxidant activity of melanoidins isolated from beer by ultrafiltration and macroporous resin chromatography was evaluated by measuring their 2,2‘-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activity and reducing power. The results showed that fraction with high molecular weight (>10 ku) exhibited low yield and melanoidins content as well as weak antioxidant activity, while the lower molecular weight of fractions possessed the higher yield, melanoidins content and antioxidant activity. The UF4 fraction (<3 ku) had the highest yield, melanoidins content and antioxidant activity. The UF4 fraction was separated into 3 fractions with different polarity (D1>D2>D3) by macroporous resin chromatography. The D1 fraction which had the highest yield accounted for almost 94% of the UF4, while the D3 fraction represented less than 1% but showed the highest melanoidins content. The D2 fraction had the strongest ABTS radical scavenging activity and reducing power, whereas D1 showed very weak antioxidant activity. Therefore, the present results indicated that melanoidins with the strongest antioxidant activity existed in UF4 fraction (<3 ku), and could be isolated from beer by macroporous resin chromatography with 20% ethanol elution.
    20  Effect of Electrodialysis Desalination on the Amino Acid Compositions and Volatile Flavor Compounds of Fish Sauce
    SUN Jin-ling GAN Zhong-hong CHEN Yu-zhu GAO Xiang-yang
    2017, 33(10):133-141. DOI: 10.13982/j.mfst.1673-9078.2017.10.020
    [Abstract](924) [HTML](175) [PDF 549.45 K](970)
    Abstract:
    Electrodialysis (ED) was utilized to desalinate the fermentation broth of traditional fish sauce. The results showed that the content of amino nitrogen, amino acids and volatile aroma compounds in the fish sauce at the target salt content of 22%, 20%, 15%, 10%, 5% and 2% (m/m), decreased nonlinearly with the increasing desalination degree when the voltage was 9 V and flow rate was 4 cm/s in electrodialysis. When the content of salt reduced to 20%, the loss rate of ammonia nitrogen was 8%, which was relatively low. While the loss rate of several bitter amino acids in free amino acids, such as tyrosine, arginine, histidine and lysine were high and the quality of all amino acids in fish sauce decreased when the degree of desalination increased, especially bitter amino acids such as amino acid, arginine, histidine and lysine. When the content of salt reduced to 2%, the loss rate was 51.60%, 49.41%, 42.41% and 31.12%, respectively, and the composition of flavor compounds was the same. The relative percentages of acids, ketones, nitrogen compounds and sulfur-containing compounds decreased while the content of 2-methyl butyraldehyde, 3-methyl butyraldehyde and benzaldehyde increased, with the increase of desalination, but were not below the taste threshold. The results of sensory evaluation showed that when the content of salt reduced to 20%, the electrodialysis desalination had little effect on the whole flavor of fish sauce, and it was beneficial to the preservation of fish sauce, which could provide guideline for the production of low-salt fish sauce.
    21  Effects of Different Freezing Methods on the Quality of Red Shrimp
    JIANG Yang-yang YANG Shui-bing YU Hai-xia YAO Jie-yu SHAO Ying CHEN Shi-guo HU Ya-qin
    2017, 33(10):142-148. DOI: 10.13982/j.mfst.1673-9078.2017.10.021
    [Abstract](884) [HTML](184) [PDF 609.33 K](821)
    Abstract:
    To improve the quality of the red shrimp (Solenocera melanth), the shrimp samples were frozen by the liquid nitrogen spray and the conventional plate-freezing. The effects of different frozen methods on the quality of red shrimp during frozen periods were investigated by physiochemical indexes, such as total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), Ca2+-ATPase activity, water holding capacity, and salt soluble protein content. The sensory scores and scanning electron microscopy (SEM) were used to compare the freezing effect as well. The results showed that TVB-N and TBA values of red shrimps gradually increased with the prolongation of storage time, but the freezing of liquid nitrogen increased slowly. After 176 d storage, the TVB-N values treated by liquid nitrogen frozen was 20.50 mg/100 g, while plate frozen reached 27.30 mg/100 g. The TBA values reached 0.74 mg/100 g, 0.97 mg/100 g, respectively, suggesting that liquid nitrogen frozen could effectively suppress the increase of TVB-N and TBA; meanwhile, the Ca2+-ATPase activity, water holding capacity, salt soluble protein content and sensory indexes of red shrimp showed a decreasing trend, while that by liquid nitrogen decreased slowly; besides, the SEM profiles revealed that myofibril of red shrimp slightly damaged by the liquid nitrogen compared with that by plate-freezing method. In conclusion, the liquid nitrogen froze showed better effects than plate frozen on the quality of red shrimp.
    22  Antioxidant Activity of Ethanol extract from Moringa oleifera Leaves
    ZHOU Wei LIU Neng LIN Li-jing LI Ji-hua GUO Chang-qing LEI Shao-ling
    2017, 33(10):149-156. DOI: 10.13982/j.mfst.1673-9078.2017.10.022
    [Abstract](983) [HTML](159) [PDF 550.84 K](937)
    Abstract:
    To optimize the extraction conditions of antioxidant components from Moringa oleifera leaves and evaluate the antioxidant activity. Based on the results of univariate experiments, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging rate was used as the evaluation index. Effects of ethanol concentration, extraction temperature, the ratio of liquid to material and extraction time on the antioxidant activity of the antioxidant components from Moringa oleifera leaves were studied by orthogonal test. The results showed that the optimal parameters of extraction were as follows: extraction temperature of 90 ℃, the ratio of liquid and material of 1:30, ethanol concentration of 60% and extraction time of 1.5 h. Under these conditions, the flavonoids and polyphenol content were 69.36±0.58 mg/g and 53.72±0.48 mg/g, respectively, and the clearance of DPPH free radical was 57.83±0.14%. The crude extracts obtained from Moringa oleifera leaves had strong scavenging capacity on DPPH, 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and hydroxyl radical (·OH) free radical in a dose-dependent manner, but its reducing power was weak. The EC50 values of crude extracts were 86, 31 and 140 μg/mL, respectively. The scavenging impact of the three radicals accounts for 98.26%, 99.04% and 96.63% of butylated hydroxytoluene (BHT), respectively.
    23  Synthesis of Surface Molecular Imprinted Polymer Based on Silica Nanoparticles and Adsorption Properties of Methyl Parathion
    LI Wei-ming TAN Lei CEN Shui-bin TANG You-wen WU Hui
    2017, 33(10):157-163. DOI: 10.13982/j.mfst.1673-9078.2017.10.023
    [Abstract](1022) [HTML](176) [PDF 808.95 K](845)
    Abstract:
    Hybrid organic-inorganic surface imprinted silica nanoparticles for specific recognition of methyl parathion was prepared by molecular imprinting technique, We molecularly imprinted silica nanoparticles in chloroform by using amino modified silica nanoparticles as the supports, acrylamide as the functional monomer, γ-methacryloxypropyl trimethoxy silane as the grafting agent, and methyl parathion as a template. The amino propyl functional monomer could not only selectively polymerize on silicon surface, but also as an assistive monomer to drive the template agent into the formed shell structure to produce effective specific recognition sites. The resultant MIPs-SiO2 nanoparticles display a three-dimensional core-shell structure and a large surface area. The molecularly imprinted polymer shell structure provided recognition sites for methyl parathion, making it have a strong selective adsorption capacity for the template, Traces of methyl parathion were selectively extracted from green vegetable and pear samples using MIPs-SiO2 nanoparticles as the sorbents of solid-phase dispersion extraction with recoveries of 84.7~94.4%.
    24  Effect of Boiled Water Drying and Vacuum Freeze Drying on the Fat-soluble Components and Color of Antler Velvet in Sika Deer (Cervus nippon)
    HOU Zhao-hua LIU Chang REN Gui-xing
    2017, 33(10):164-170. DOI: 10.13982/j.mfst.1673-9078.2017.10.024
    [Abstract](785) [HTML](202) [PDF 563.17 K](953)
    Abstract:
    The effects of processing technologies (vacuum freeze-drying and boiled water-drying) on the fatty acids, cholesterol and color of antler velvet in sika deer (Cervus nippon) were investigated in this study. The results identified that there were sixteen fatty acids in vacuum freeze-drying antler velvet (VFDA) and thirteen in boiled water-drying velvet antler (BWDA). The total content of fatty acid was significantly higher (p<0.05) in VFDA than in BWDA. The atherogenic index (AI) in BWDA and VFDA was 1.95 and 1.77, and thrombogenic index (TI) values in BWDA and VFDA were 2.44 and 2.32, respectively. The content of cholesterol in VFDA and BWDA were 2.95±0.16 and 2.67±0.09 mg/ g, respectively. The values of a*, b* and h in VFDA were significantly different (p<0.01) in BWDA. The values of a* in VFDA were significantly (p<0.01) higher than in BWDA, while b* and h in VFDA were significantly lower than in BDWA. This showed that VFDA was brighter, more red and blue than BWDA. These results suggested that the compositions of antler velvet were strongly influenced by processing technologies, and vacuum freeze drying was an effective technique to preserve the fat-soluble components and aberration of drying velvet antler.
    25  Study on the Extraction of Natural Pigment from Maize Yellow Powder with Sunflower Oil Microemulsions
    WU Hong-yan SUN Chang-bao YU Xiao-dan JIA Xiu-feng
    2017, 33(10):171-177. DOI: 10.13982/j.mfst.1673-9078.2017.10.025
    [Abstract](766) [HTML](146) [PDF 715.92 K](824)
    Abstract:
    The food-grade microemulsions and its application in the extraction of natural edible pigment were investigated in this study. Based on the single factor experiment, solid/liquid ratio, temperature and extraction time were chosen as the factors to optimize technological conditions by design three-level response surface experiment. The optimum process conditions for extracting natural edible pigment from sunflower oil microemulsion were determined, and then the properties of the sunflower oil microemulsion were characterized after extracting. The results showed that the order of factors influencing the extraction rate of pigment was solid/liquid ratio > temperature > time. Using the response surface and SAS software to analyze the data, and the optimal extraction conditions were as follows: the ratio of solid to liquid 1:42, temperature 71 ℃, and time 1.5 h. The actual value of extraction rate of pigment was 0.34 mg/g under the optimal extraction conditions, which had no significant difference with the theoretical value. The sunflower oil microemulsion had good performance after extracting, and its particle size had no significant change and remained good stability.
    26  Effects of Different Temperature and Packaging on the Storage of Chick Peach
    JIANG Jun LUO Fang-zeng LIU Jian-liang LUO Yun-fang QIU Yuan-xin TANG Mei WANG Qin
    2017, 33(10):178-185. DOI: 10.13982/j.mfst.1673-9078.2017.10.026
    [Abstract](829) [HTML](268) [PDF 667.91 K](798)
    Abstract:
    The effects of three kinds of packaging materials associated with PVC, PE and NP on the changes of respiration, ethylene release, firmness, weight loss rate, good fruit rate, soluble solids, titratable acid and vitamin C of postharvest chick peach storaged at room temperature and low temperature (2±1 ℃) were investigated in this study. The results showed that low temperature and packaging of PVC, PE and NP could inhibit the respiration intensity of the fruit, reduce the release of ethylene, improve the rate of good fruit and maintain good storage quality. The effect of PVC combined with low temperature storage was the best, which delayed the time of respiration climacteric and the release time of ethylene peak for 4 days at the same time and reduced the respiration and ethylene release rate. After storage for 30ds, the content of soluble solids in Chick peach remained at 9.51%, titratable acid content was 0.23%, vitamin C content was 5.90 mg/100 g, fruit hardness was 8.20 kg/cm2, fruit weight loss rate was 3.10%, and good fruit rate was 84.50%.
    27  Extraction of Pericarpium Zanthoxyli Essential Oil with High Content of Numb-Taste Components by Subcritical Fluid
    ZHONG Lian-jun ZHANG Deng-hui MENG Xiang-dong YANG Ji-guo
    2017, 33(10):186-191. DOI: 10.13982/j.mfst.1673-9078.2017.10.027
    [Abstract](871) [HTML](224) [PDF 449.19 K](944)
    Abstract:
    Subcritical fluid was used in this study to extract Pericarpium Zanthoxyli essential oil from zanthoxylum L. peels and the effects of different process conditions and fluid medium on the yield of essential oil and extraction rate of numb-taste components were investigated. The optimal extraction conditions using subcritical butane as the solvent were as follows: extraction temperature, 40 ℃; extraction time, 40 min; extraction times, 4. Under these conditions, the essential oil extraction yield was 11.11% and the extraction rate of numb-tasted components was 59.81%. The yield of essential oil increased to 11.96%~12.68% when anhydrous ethanol was added at a volume fraction of 4~6% in subcritical butane and the extraction rate of numb-taste components was 91.96%~96.56%., Impurities content in Pericarpium Zanthoxyli essential oil increased significantly with the increasing concentrations of ethanol in the mixed fluid medium which resulted in a poor quality of the products. Subcritical butane had a good effect on extracting volatile oil in Pericarpium Zanthoxyli while an opposite effect on extraction rate of numb-taste components. The mixed subcritical medium had a high-efficient extraction of volatile oil and numb-tasted components from Pericarpium Zanthoxyli simultaneously with a low temperature, which allowed obtaining the Pericarpium Zanthoxyli essential oil with a high content of numb-taste components. The proposed method had an important value for practical application.
    28  Prolonging Shelf Life of Fish (Sea catfish) by the Additions of Combined Bio-preservatives
    LING Ze-xing SUN Man-yu ZHONG Cheng JIA Shi-ru TAN Zhi-lei
    2017, 33(10):192-200. DOI: 10.13982/j.mfst.1673-9078.2017.10.028
    [Abstract](801) [HTML](181) [PDF 776.17 K](1419)
    Abstract:
    Seafood is susceptible to spoilage due to its high concentration of nutrition. Microorganisms play a key role in the spoilage of most seafood. Eight microbial strains were isolated from spoiled Sea catfish in this study, which were identified to be Exiguobacterium acetylicum, Acinetobacter johnsonii, Kocuria sp., Arthrobacter creatinolyticus, Pseudomonas stutzeri, Bacillus subtilis, Psychrobacter celer and Acinetobacter lwoffii. Minimal inhibitory concentrations (MIC) of 3 bio-preservatives (ε-polylysine, Nisin and lysozyme) on the isolated strains were determined. The inhibitory of total bacteria counts by combined bio-preservatives were optimized by response surface methodology. The optimized concentration of ε-polylysine, Nisin and lysozyme was 367.5, 375.0 and 235.0 mg/kg, respectively. The combined bio-preservatives damaged the bacterial cell membrane integrity and increased bacterial membrane permeability of Bacillus subtilis, evidenced by scanning electron microscopy. Total bacteria counts, pH value and TVB-N of Sea catfish with an optimized combined bio-preservative were determined to verify the antibacterial effect, which showed shelf life of Sea catfish with combined bio-preservatives was about twice as long as control both stored at 4 ℃.
    29  Optimization of Ultrasonic-Assisted Extraction of Pectin from Premna microphylla Leaves and Analysis of Product Properties
    BAO Li-jun KE Fu SUN Kai LI Zhu-lin
    2017, 33(10):201-207. DOI: 10.13982/j.mfst.1673-9078.2017.10.029
    [Abstract](1077) [HTML](174) [PDF 616.19 K](858)
    Abstract:
    Premna microphylla leaves were wildly existed in Qinba Mountain with high pectin content and low degree of esterification. In order to make full use of Premna microphylla leaves, the effects of different solvents-pH, extraction temperature and ultrasonic power on the yield of pectin extraction were investigated. Response surface methodology was employed to optimize the pectin extraction conditions from Premna microphylla leaves. The mathematical model was established according to Box-Behnken central composite design. Besides, the fractionally precipitates with ethanol concentration of 30% and 50% respectively were produced from the optimized extraction products, and their physicochemical properties and infrared spectrum were studied. The results showed that the fitted quadratic regression model was valid. The optimum extraction parameters were pH 1.9, extraction temperature 70 ℃, and ultrasonic power 630 W. Under these conditions, the maximal rate of pectin extraction yield was 23.16%, which was close to the predicted value with 23.20%. The pH and ash value of 30% and 50% fractionally precipitates were in accordance with the requirement of QB 2484-2000 and their typical characteristics were also shown by infrared spectrum.
    30  Staphylococcus aureus Drug Resistance of Dairy Cow Mastitis in Tianjin Region
    TIAN Xiao-ying WANG Jun YU Zhong-na ZHEN Tian-yuan WANG Shi-qing HAN Rong-wei
    2017, 33(10):208-216. DOI: 10.13982/j.mfst.1673-9078.2017.10.030
    [Abstract](886) [HTML](180) [PDF 718.87 K](799)
    Abstract:
    To understand the pathogenicity of Staphylococcus aureus in dairy cow mastitis and the sensitivity to antibiotics in Tianjin area. Mannitol salt medium and high lyophilized rabbit plasma were used for isolation and identification of 50 samples that were collected from seven cattle farms in Tianjin to provide scientific references for treatment of dairy cow mastitis and prevention of veterinary drug residues, and the susceptibility tests were performed by disk diffusion method. 29 Staphylococcus aureus and 4 other pathogenic Staphylococci were isolated and identified. Drug resistance of isolated Staphylococcus aureus were analyzed with 8 categories of 29 species commonly used antibiotics. Staphylococcus aureus isolates were amplified by PCR of the drug resistance gene and detected by agarose gel electrophoresis. The result showed that 29 isolates of Staphylococcus aureus had different resistance to 8 categories of 29 species antibiotics. The drug resistance rates to macrolides and lincosamides were generally high, which were 77.01% and 89.66% in average, and the drug resistance rates to β-lactams, quinolones and aminoglycoside were all 50% below. The isolates had the complete resistance (100%) to penicillin G, Acetyl-Lo and lincomycin, and the 20%~40% resistance rates to cefoperazone, cefotaxime, amoxicillin, ampicillin, enrofloxacin, neomycin, spectinomycin. Analysis of the drug resistance gene showed that the positive rate of drug resistance gene was generally less than that of phenotypic rate of drug resistance gene.
    31  Comparison of the Sensory Characteristics and Aroma Components of Fu Brick Tea for Sale in Border Areas of Different Regions
    ZHAO Ren-liang XU Wei WU Dan JIANG Yi-he ZHU Qi
    2017, 33(10):217-224. DOI: 10.13982/j.mfst.1673-9078.2017.10.031
    [Abstract](779) [HTML](166) [PDF 524.54 K](931)
    Abstract:
    To study the aroma quality characteristics of Fu brick tea for sale in border areas of different regions in our country, tea samples from representative production areas (including Shanxi, Hunan and Zhejiang province) were used as the research objects. The aromatic components of Fu brick tea were analyzed by simultaneous distillation extraction (SDE) technique combined with gas chromatography-mass spectrometry (GC-MS), whilst we used the sensory evaluation to preliminary prove the aroma characteristics of Fu brick tea. The main aroma features of Fu brick tea were pure, but Fu brick tea from Shanxi had high fire aroma, Fu brick tea from Hunan had arohid flavor, and Fu brick tea from Zhejiang had slight grass odour. GC-MS analysis indicated that there were 46, 56 and 53 kinds of aroma components identified in Fu brick tea from Shanxi, Hunan and Zhejiang province, respectively. 45 kinds of aroma components among them mainly included alcohols, aldehydes and ketones, which accounting for 65.22%, 69.01% and 73.22% of the total aroma in Fu brick tea from Shanxi, Hunan and Zhejiang province, respectively. The major volatile common components were hexadecanoic acid, phytol, nerolidol, 6,10,14-trimethyl-2-pentadecanone, (E,E)-2,4-heptadienal, geranylacetone, beta.-Ionone, etc. The roast incense heterocyclic compounds with high fire aroma were the highest content in Shanxi Fu brick tea, the content of olefin aldehyde compounds were the highest in Hunan Fu brick tea, and the content of the lower aliphatic aldehyde with grass odour were the highest in Zhejiang Fu brick tea, which were consistent with the results of sensory evaluation.
    32  Comparative Study on the Phenolic Profiles of Eight Fruits and Their Antioxidant Activities
    ZHAO Mou-ming DONG Hong-zhu LIN Lian-zhu
    2017, 33(10):225-236. DOI: 10.13982/j.mfst.1673-9078.2017.10.032
    [Abstract](870) [HTML](789) [PDF 650.09 K](1189)
    Abstract:
    The content of vitamin C and total phenolic content in peel, flesh, whole fruit and fruit juice of eight fruits were determined in this study. Phenolic profiles of eight fruits were determined by high performance liquid chromatography (HPLC), and their antioxidant activities were evaluated. The results showed that the content of vitamin C in eight fruits were in the order of guava > navel orange > pineapple = papaya > green apple = crown pear = kumquat = mango. Mango had the highest total phenolic content. Sixteen phenolic compounds were identified from eight fruits, and the content of chlorogenic acid, catechins, epicatechin, rutin, myricetin and phloridzin were higher. Eight fruits were divided into four categories in terms of the phenolic profiles: (1) navel orange; (2) mango; (3) kumquat; (4) green apple, crown pear, guava, pineapple and papaya. DPPH radical scavenging activities of the eight fruits were in a descending order as follows: mango > guava > green apple > crown pear = kumquat = navel orange = pineapple = papaya. Oxygen radical absorbance capacities decreased in the order of navel orange > guava > crown pear = mango > green apple = kumquat = pineapple = papaya. This study could provide the theoretical guidance for the development of fruit deep processing industry and functional food.
    33  Analysis of Biogenic Amines in Pickled Vegetable by a New Pre-column Derivatization of RP-HPLC
    GUO Xiao-li LI Er-hu WANG Lu-feng LIU Qi LI Xin-yu YU Jia ZHONG Wu SONG Ya-dong PAN Si-yi
    2017, 33(10):237-244. DOI: 10.13982/j.mfst.1673-9078.2017.10.033
    [Abstract](961) [HTML](225) [PDF 605.08 K](834)
    Abstract:
    A new pre-column derivatization of reversed-phase high performance liquid chromatographic (RP-HPLC) method for the determination of eight biogenic amines (histamine, tryptamine, β-phenylethylamine, tyramine, putrescine, cadaverine, spermidine and spermine) in pickled vegetable was established using 4-fluoro-3-nitrobenzo-trifluoride (FNBT) as the derivatizing agent and N,N-diisopropylethyl-amine (DIPEA) as the catalyst. The derivatization of biogenic amines was conducted by FNBT and DIPEA with borate buffer solution at 60 ℃ for 30 min, and then the resultant mixture was separated on a C18 column with mobile phase (acetonitrile and ultrapure water) at a flow rate of 1.00 mL/min. The UV detection wavelength was 242 nm. Eight biogenic amines showed a good linear correlation (r2≥0.999) in the concentration range of 0.5~50 mg/L with the limits of detection (LODs) of 0.013~0.053 mg/L and the limits of quantification (LOQs) of 0.044~0.176 mg/L. The mean recovery was in the range of 93.47%~99.87%, and the relative standard deviations (RSDs) were 0.47%~3.67%. This method was used to analyze the biogenic amines in the 26 commercial pickled vegetable, and the results showed that the total content of biogenic amine in fermented and non-fermented pickled vegetable samples were 40.57~692.82 mg/kg and 9.86~270.93 mg/kg, respectively; the average content of eight monomer biogenic amines in fermented pickled vegetable samples were higher than those of non fermented pickled vegetable samples. This developed method was simple, sensitive and reliable, and it was suitable for the rapid analysis of biogenic amines in pickled vegetable.
    34  Determination of the 56 Pesticide Residues in Vegetables by QuEChERS-ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
    LI Xin SUN Su-qun ZHANG Wei-feng CHEN Wei
    2017, 33(10):245-253. DOI: 10.13982/j.mfst.1673-9078.2017.10.034
    [Abstract](832) [HTML](235) [PDF 579.13 K](976)
    Abstract:
    A new method for analysis of 56 pesticide residues in vegetables using modified QuEChERS-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was established to solve the technical difficulties of complex components, interference and low concentration. Vegetable samples were pretreated bymodified QuEChERS, which was extracted with acetonitrile containing 1% (V/V) acetic acid, and then salted out with sodium chloride and anhydrous sodium. purified with primary secondary amine, samples was analyzed by HPLC-MS/MS. The extract was separated on a C18 column (4.6×100 mm, 2.6 μm) using acetonitrile and aqueous solution as the mobile phase (containing 5 mM ammonium acetate and 1% (V/V) acetic acid), ionized in electrospray ion (ESI+) mode and then analyzed in multiple-reaction monitoring (MRM) mode. The results showed that the 56 pesticide residues showed good linear correlation in the range of 2.0 μg/L to 100 μg/L with the correlation coefficients over 0.99. The limits of detection were less than 0.01 mg/kg, and the recoveries were ranged of 65.8%~114.0% at three spiked levels in vegetables with the relative standard deviations of0.1%~14.5%. This proposed method is rapid, simple, low-cost and effective, which can be applied to rapid screening and analysis of pesticide residues in vegetables for the risk assessments of quality and safety.
    35  Speciation Analysis of Calcium, Iron, and Zinc in Three Kinds of Health-care Food by Ultrafiltration-Atomic Absorption Spectrometry
    CHEN An-hui WU Yong-hua LIU Hui XING Jian-chao OU Li-hu CHEN Shang-long
    2017, 33(10):254-261. DOI: 10.13982/j.mfst.1673-9078.2017.10.035
    [Abstract](692) [HTML](187) [PDF 544.79 K](776)
    Abstract:
    Amethod was developed for the speciation analysis of metal elements in health-care food by ultrafiltration-atomic absorption spectrophotometry. The suspended and soluble metal elements in health-care food were obtained by 0.45 μm filtration membranes, and the inorganic and organic metal elements in soluble species were separated by D101 macroporous resin. Moreover, the organic-form metal elements were separated based on the molecular weight with ultrafiltration membranes. The content of calcium, iron and zinc in different species were determined by microwave digestion-high resolution-continuum source flame atomic absorption spectrometry. The results showed that the three kinds of health-care food were all rich in calcium, iron, and zinc. Most of them were in soluble form, of which the organic forms were more than half. The organic calcium of oral liquid including calcium, iron and zinc was mainly distributed in the fraction with molecular weight< 300 ku, while the organic iron and zinc were mainly distributed in the fraction with molecular weight< 8 ku. Most of the organic-form calcium and iron of oral liquid including polygonatum and enzymes were distributed in the molecular weight<300 ku fraction, while the organic zinc was mainly distributed in the molecular weight < 8 ku . All the organic form of them in solid including enzyme were mainly distributed in the molecular weight <300 ku fraction. Therefore, the proposed method has favorable practical value. It provides much basic data for the further study on the functions of the health-care food, especially for the biological effectiveness research of beneficial metals.
    36  Determination of Forchlorfenuron and Its Metabolites in Kiwifruit Using QuEChERS-LC-MS/MS
    ZHANG Zhi-wei GAO Zhen-hong YUAN Ya-hong WANG Yuan YUE Tian-li
    2017, 33(10):262-267. DOI: 10.13982/j.mfst.1673-9078.2017.10.036
    [Abstract](844) [HTML](168) [PDF 586.79 K](896)
    Abstract:
    Forchlorfenuron (1-(2-chloro-4-pyridyl)-3-phenylurea) is a plant growth regulator, which mainly be used to increase the size and weight of kiwifruit. The standards of forchlorfenuron metabolites were obtained by chemosynthesis and forchlorfenuron and its metabolites (4-hydroxyphenyl- forchlorfenuron, 3-hydroxyphenyl-forchlorfenuron, forchlorfenuron-4-O-β-D-glucoside and forchlorfenuron-3-O-β-D- glucoside) in kiwifruit were simultaneously determined by LC-MS/MS with ESI+ under the mode of multiple reaction monitoring (MRM) in this study. The pre-treatment of sample was improved by optimizing PSA addition with acetonitrile as extraction solvent for extraction of clopidourea residue. The results showed that forchlorfenuron and its metabolites had a good linear correlation within a certain concentration range, the relative standard deviations were below 7.1% with a good repeatability, the limit of detection (LOD) was 1~3 μg/L with a limit of quantification (LOQ) of 5~10 μg/L, and recoveris were between 87% and 108% with the PSA addition of 400 mg/15 mL. Therefore, QuEChERS-LC-MS/MS was sensitive and accurate, which could be used for simultaneous determination of forchlorfenuron and its metabolites in kiwifruit.
    37  Banana Maturity Characteristic Prediction Based on Hyperspectral and PCA-ELM
    LI Yuan-hong WANG Hong-jun DENG Jian-meng LI Zou-zou ZHOU Wei-liang JIN Jun-dong
    2017, 33(10):268-274. DOI: 10.13982/j.mfst.1673-9078.2017.10.037
    [Abstract](997) [HTML](180) [PDF 637.82 K](1012)
    Abstract:
    Spectral data of 450 unbarked banana samples, stored at room temperature, were collected by hyperspectral imaging tecchique. The single factor analysis of variance was used to measure the soluble solid content (TSS) and firmness (FIM), and then a spectral and image feature classification model of banana maturity physicochemical index was established using partial least squares linear regression analysis method of ridge optimization (RR-iPLS). The results showed that the correlation coefficient values (R2) of the soluble solid content of spectral data predicting banana and the firmness were 0.92 and 0.94. Then, characteristic wavelengths were selected by continuous projection method (successive projections algorithm, SPA) and principal component analysis (principal component, analysis, PCA) and the extreme learning machine (extreme learning machine, ELM) was established for the cross validation of spectral data based on characteristic wavelengths. , The PCA-ELM classification model based on characteristic wavelength had a better prediction performance with a high accuracy by comparing the RR-iPLS, SPA-ELM and PCA-ELM classification prediction models. The accuracy of cross validation reached to 99%, which could provide an effectively predictive study for the rapid and non-destructive identification of banana quality. The proposed method basically fulfilled the classification and detection of banana maturity and could achieve effective economic benefits.
    38  Determination of Quaternary Ammonium Compounds in Milk Powder by QuEChERS Purification Coupled with UPLC-MS/MS
    YAO Rui-xiong
    2017, 33(10):275-280. DOI: 10.13982/j.mfst.1673-9078.2017.10.038
    [Abstract](763) [HTML](214) [PDF 424.23 K](819)
    Abstract:
    Quaternary ammonium compounds (QACs) were commonly used as the disinfectants to sterilize the production equipment during the production process of dairy products, which resulted in the QACs contamination in dairy products. A new method for the determination of five QACs in milk powder was established using QuEChERS purification coupled with ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Milk powder samples were dispersed into pro-rated water to obtain the liquid milk, and then precipitated by acetonitrile. The extract was analyzed in multiple-reaction monitoring (MRM) mode of MS/MS after purifying with PSA and separating with hydrophilic column (HILIC). Consequently, the five QACs showed a good linear relationship in concentration ranges of 0.2~50 μg/L, with the correlation coefficients over 0.999. This method was validated in terms of linearity, sensitivity, precision, accuracy. The limits of detections (LODs, S/N=3) for milk powder samples were in the range of ranged from4.0-14.4 μg/kg, and the recoveries were in the range of 96.5% - 115%.The relative standard deviations (RSDs) of intra-day precision and inter-day precision were 3.1-7.5% and 4.3~6.7%, which indicated that there were QACs residues in milk powder The proposed method demonstrated accuracy, sensitivity, rapidity, simplicity and good precision, which was suitable for the analysis and detection of five QACs in milk powder and other dairy products.
    39  Study on Varieties Discrimination of Nectarine by Hyperspectral Technology Combined with CARS-ELM Algorithm
    ZHAO Xu-ting ZHANG Shu-juan LIU Jiang-long SUN Hai-xia
    2017, 33(10):281-287. DOI: 10.13982/j.mfst.1673-9078.2017.10.039
    [Abstract](983) [HTML](251) [PDF 627.52 K](866)
    Abstract:
    The varieties discrimination feasibility of nectarines by hyperspectra technology combined with competitive adaptive reweighted sampling (CARS) and extreme learning machine(ELM) were discussed. Hyperspectra imaging technology was used to select the hyperspectra images data of three different types of nectarines in the range of 420 to 1000nmin this study.The raw spectra were processed by Savitzky-Golay, Multiplicative Scatter Correction, Baseline, and Standard Normalized Varite. The most appropriate pretreatment method was confirmed by the PLSR model. In order to extract characterized wavelengths, there are three different approaches have been used: principal component analysis (PCA), successive projections algorithm (SPA), and CARS. Furthermore, partial least square(PLS)and extreme learning machine (ELM) on the basis of three different characterized wavelengths were used to build the model for identifying the species of nectarines. The results of tests implicated that the performance of CARS-PLS and CARS-ELM classification model was optimized using optimal characterized wavelengths of CARS algorithm. The PLS and ELM model correlation coefficient (Rp), the root mean squared error (RMSEP) of prediction set were 0.942, 0.205 and 0.931, 0.119, respectively. It indicated that CARS algorithm was one of the effective methods for exacting the characterized wavelengths,and ELM model prediction ability was equivalent with conventional PLS model. It approves that the hyperspectral imaging technology combined with CARS-ELM is feasible to discriminate the nectarine varietiesn.
    40  Detection and Correlation Analysis of Dry Matter, Starch and Reducing Sugar Content in Potato Tubers
    ZHAO Yu-ci XU Dan JIN Cheng-yu ZENG Fan-kui LIU Gang
    2017, 33(10):288-293. DOI: 10.13982/j.mfst.1673-9078.2017.10.040
    [Abstract](1948) [HTML](400) [PDF 412.84 K](1237)
    Abstract:
    In this study, dry matter, reducing sugars and starch content of forty-six potato varieties from 13 provinces (14 regions), were detected according to GB methods. The significances of reducing sugar and starch content amongst different samples were analyzed by SPSS 20.0, and the correlation amongst dry matter, reducing sugar and starch content were studied by scatterplot matrix and simple line regression plot. The results showed that the content of dry matter in potato was 15.43%~29.67%, the content of reducing sugar was 0.09%~1.84% and the content of starch was 9.11%~22.30% (all wet basis). The content of reducing sugars amongst most of different potato varieties was significant difference (p<0.05), and similar result was found in starch content. While there was no correlation between reducing sugar content and dry matter, or between reducing sugars content and starch content, according to the scatterplot matrix analysis. Potato dry matter content and starch content were positively correlated with the linear equation: y=9.626+0.708x, which means that the higher the dry matter content in the potato, the higher the starch content.
    41  Analysis of Heterocyclic Amines in the Traditional Meat Products Determined by HPLC
    LI Ke HAN Xue XIE Mei-juan LI Jun-guang HE Xiang-li ZHAO Dian-bo BAI Yan-hong
    2017, 33(10):294-301. DOI: 10.13982/j.mfst.1673-9078.2017.10.041
    [Abstract](851) [HTML](229) [PDF 552.32 K](898)
    Abstract:
    The content of heterocyclic amines (HAs) in traditional sauce halogen meat was detected by high performance liquid chromatography (HPLC) to evaluate the safety of traditional meat products in this study. The content of HAs and basic chemistry components in the meat were studied through their different kinds of meat products, processing technology conditions and location sources of sampling..The results showed that the basic chemistry components in different meats had significant differences .Content of HAs was higher in the individual stalls than in supermarkets and exclusive sales. Content of HAs was higher in sauce halogen-roasted and roasted than other processing technologies, which reached 13.40 μg/kg and Norharman and Harman were detected in all samples. Content of HAs in skin was higher than in chicken , and the content of HAs in skin of sauce pot stewed chicken legs, chicken sauce halogen and Beggar's chicken was 2.8, 1.5, 1.5 times in chicken, respectively.
    42  Comparison of Eleutheroside B , Eleutheroside E, and Isofraxidin between Acanthopanax sentcosus (Ruper.et Maxim.) Harms and Acanthopanax sessiliflorus
    TAN Wu-feng YUAN Yan-qiu YU Di-di ZHANG Bin-wang WANG Jian-li LUAN Guang-zhong
    2017, 33(10):302-307. DOI: 10.13982/j.mfst.1673-9078.2017.10.042
    [Abstract](1194) [HTML](198) [PDF 551.67 K](1708)
    Abstract:
    Thin Layer Chromatography (TLC) was used to identify the differences of main active components between Acanthopanax sentcosus (Ruper.et Maxim.) Harms (ASR) and Acanthopanax sessiliflorus (AS), which were obtained from Jilin, Heilongjiang and Liaoning. The content of Eleutheroside B, Eleutheroside E and Isofraxidin were determined by High Performance Liquid Chromatography (HPLC). The results showed that the Isofraxidin characteristic band of ASR displayed on TLC chromatogram was obvious using a ultraviolet lamp (365 nm). While the Isofraxidin characteristic band of AS was not obvious, it still existed on TLC chromatogram. Consequently, the identification method could improve quantitative indicator that was to give a definite conclusion. The HPLC results showed that the content of three functional components in ASR samples were high, and the average content of Eleutheroside B and Isofraxidin of ASR samples were higher than that of AS samples. The content of Eleutheroside B of several AS samples was nearly 0.1%, which was higher than the level regulated by Chinese Pharmacopoeia (0.05%). The content standard of Eleutheroside B could be improved for further study to avoid the adulterant of ASR.
    43  Advance of the Removal Technology of Heavy Metals in Foods
    YE Meng-qi DU Zong-jun CHEN Guan-jun
    2017, 33(10):308-318. DOI: 10.13982/j.mfst.1673-9078.2017.10.043
    [Abstract](1351) [HTML](218) [PDF 564.31 K](1150)
    Abstract:
    Heavy metal poisoning is harm to multiple organs and multiple systems of human bodies, which is lifelong and irreversible. The food safety problems caused by heavy metal pollution are much more serious than the other pollution factors. Heavy metals cannot be biodegraded, insteadthey can be enriched tens of thousands of times through biological amplification of the food chain, and enter the body finally. The three wastes from the industries of energy, transportation, metallurgy and material, as well as the pesticides and fertilizer in the agriculture activity, are all the causes of food contamination. Controlling the heavy metals contamination from the source can solve the problem completely, but it is difficult to finish in a short time. Therefore, removal of heavy metals in foods effectively becomes a fatal question and a research focus at home and abroad. Based on the analysis and summary of existing research literature, this paper combed the research methods and technology. The removal technology of heavy metals in the food raw materials during growth process and before food consumption was discussed, respectively. In addition, the application and efficiency of the established methods were summarized and analyzed, and the future development trend and prospect were also discussed.

    Editor in chief:李琳

    Inauguration:现代食品科技

    International standard number:ISSN 1673-9078

    Unified domestic issue:CN 44-1620/TS

    Domestic postal code:46-349

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