2014, 30(2):12-16.
Abstract:
Fifty female ICR mice were randomly assigned to five groups, individually fed on commercial diets，high-fat diet, high-fat diet with low, middle, high level of water extraction from Smilax china (WESC) for 8 weeks,. Body weight and food intake were inspected regularly during this period; after eight weeks, the weights of body, liver and intraperitoneal adipose tissue (IPAT) were measured, and the serum index and the activities of liver lipid metabolism related enzymes in mice were analyzed. Compared with the high-fat group, low, middle and high doses of WESC significantly decreased body weight gain, IPAT weight and the concentrations of GLU and TC in serum. The body weight gain decreased by 31.97%, 38.31% and 36.90%, respectively. IPAT weight decreased by 41.34%, 48.58% and 43.41%, respectively. The concentration of GLU reduced by 28.50%, 17.15% and 15.10%, respectively, and the TG concentration reduced by 35.86%, 29.31% and 46.90%, respectively. The activity of ACO in liver were increased by 25.49%, 29.41% and 26.47%, respectively. And the middle and high doses of WESC improved the activity of AMPK by 29.17% and 33.33%, respectively. The above results showed that the WESC suppressed fat accumulation and decreased the weight gain in mice, which was mainly due to increase of the activity of fat oxidation enzyme in liver, promotion of the fatty acid β-oxidation. No obvious influence was observed on fat synthesis.
