Volume 29,Issue 8,2013 Table of Contents

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  • 1  Identification of Tumor Antigen in the Breast Cancer Vaccine
    LIU An-jun FANG Jing-jing MA Shan-jie
    2013, 29(8):1751-1755.
    [Abstract](1285) [HTML](0) [PDF 550.60 K](720)
    Abstract:
    The tumor antigen in the breast cancer vaccine which activated the anti-tumor immunity response was determined according to the antigen-specific antibodies existent in the serum of the immunized mice by immunoproteomics analysis. The results showed that the antibody titer of immunized mice serum was over 6400 and the high antibody titer suggested that the humoral immunity on anticancer was strengthened. SDS-PAGE and western-blot analysis showed that the protein extracted from Ca761 cells treated with cartilage polysaccharide was bonded with the immune serum at an estimated molecular mass of 50 kDa. 2D-PAGE and western-blot analysis showed that the tumor antigens activated anti-tumor immune response were acidic protein at 50 kDa. By MALDI-TOF-MS, the reactive protein was identified as α-tubulin. In summary, structure and expression of tumor antigen (α-tubulin) in Ca761 cells was modified with the cartilage polysaccharide treatment, therefore, the anti-tumor immunity of mice was activated by the tumor vaccine so that the mice immunized with the tumor vaccine could prevent from invasion of tumor cells. The immunogenic of α-tubulin was strengthened after the treatment with cartilage polysaccharide, which could be developed as a new vaccine.
    2  Cloning of Phytoene Desaturase Gene from Dunaliella bardawil and Promoter Activity Analysis
    JIANG Jian-guo CHEN Shan-li LAO Yong-min
    2013, 29(8):1756-1760.
    [Abstract](1259) [HTML](0) [PDF 488.33 K](790)
    Abstract:
    Phytoene desaturase (PDS) is the upstream key enzyme of carotenoid metabolic process of Dunaliella bardawil. According to the mRNA that NCBI published before (GenBank: Y14807), the PDS coding region was obtained through genome walking and nesting PCR. Due to that 325 bp in 5’ coding region were not matched with the mRNA published before., 5’ RACE was used to validate the 5’ sequences and the final result of 5’ RACE was matched with the 5’ sequences isolated. The sequence of promotor and terminator were also obtained by genome walking. The full length of PDS gene isolated included 8113 bp coding region (From ATG to TAA), 3010 bp upstream sequence of coding region and 1555 downstream sequence. 12 Exons and 11 introns were found in the cDNA ORF and coding region. Several kinds of transcription factor binding sites were predicted in the promotor of PDS by bioinformatic software.
    3  Synthesis and Adsorption Property of Bitertanol Molecularly Imprinted Polymer
    GAO Wen-hui LIU Bo YIN Hang WANG Jiao-jiao JIA Ying-min
    2013, 29(8):1761-1769.
    [Abstract](1342) [HTML](0) [PDF 491.12 K](658)
    Abstract:
    A bitertanol molecularly imprinted polymer (BMIP) was prepared by the method of bulk polymerization under different ratios between template molecular and functional monomer, using bitertanol as template molecule and methacrylate acid (MAA) as functional monomer and its specific recognition ability was studied. The influence of different pore-foaming agents on the interaction between template molecular and functional monomer was investigated. The effect of the prepared polymer using different ratios template molecular and functional monomer on the adsorption content of bitertanol was also studied. Adsorption property of bitertanol was examined by static adsorption test and analyzed by Scatchard analysis. The maximum absorption wavelength of bitertanol by using acetonitrile and tetrahydrofuran as pore-foaming agents showed 5 nm and 6 nm red shifts, respectively, and its absorption intensity increased. Scatchard analysis showed that bitertanol template molecule and functional monomer MAA formed two types of binding sites and the dissociation constants of the binding sites were 3.16 mmol/L and 107.53 mmol/L, respectively. Tetrahydrofuran and acetonitrile were more suitable for the preperation of BMIP. The molecularly imprinted polymer can be used as the separation media of bitertanol with high affinity and good specific recognition ability.
    4  Isolation and Identification of Endophytic Fungi Associated with Pavona Lamarck of Xuwen Natural Reserve
    LEI Xiao-ling LI Jun XIAO Sheng-lan ZHONG Min
    2013, 29(8):1766-1769.
    [Abstract](1327) [HTML](0) [PDF 511.04 K](766)
    Abstract:
    To study the diversity of fungi derived from Pavona lamarck, spread-plate method and two different media were used to cultivate and isolate the strains. The strains was identified from the colony morphology, the individual form of mycelium and spores referring to fungi identification manual. Molecule was identified according to the ITS-rRNA gene sequence features. Based on the result of sequencing, a phylogenetic tree was build for population diversity analysis. 10 symbiotic fungis were isolated from the Pavona lamarck, belonging to 5 generas including 3 Aspergillus spp., 3 Hypocrea spp., 2 Penicillium spp., 1 Cladosporium spp. and 1 Phlebia spp., indicating that Aspergillus and Hypocrea were the dominant fungi. Compared to other corals, the fungis isolated from Pavona lamarck were fewer, but had a high degree of uniformity. There was no report on marine fungis.
    5  Antibacterial Activity of Pu’er Tea Extracts in Vitro
    HU Yiong-jin HAN Xiao-xi Xue Qiao-li YANG Hua-song
    2013, 29(8):1770-1773.
    [Abstract](1285) [HTML](0) [PDF 569.32 K](751)
    Abstract:
    The antibacterial activity of the solvent extracts from Pu’er tea in vitro was studied in this paper. Filter paper disc diffusion method and minimal inhibition concentrations (MIC) were employed to determine the antibacterial activities of Pu’er tea extracts by water, 75% and 95% ethanol on S. aureus, E. coli, P. aeruginosa and B. subtili s. The 95% ethanol extracts showed the highest antibacterial effect, especially to S. aureus and E. coli.with the inhibition radius being of 19.58 ± 0.24 mm and 14.58 ± 0.24 mm, respectively. The MIC was 0.63 mg/mL, 0.39 mg/mL. But 95% ethanol extracts showed little inhibitory effect on the growth of B. subtilis. The extracts obtained using different solvent showed different antibacterial effects, amont which the ethyl acetate extracts showed the strongest antibacterial activities, followed with the extracts of n-butyl alcohol, petroleum ether and chloroform. For different bacteria tested, the ethyl acetate extracts showed the strongest effects on the growth of S. aureus with the inhibition zone diameter being of 31.88 ± 0.24 mm and the MIC being of 0.473 mg/mL. The ethyl acetate extract of Pu'er tea could significantly inhibit the growth of gram-positive and gram-negative bacteria. This study provided the references for the development and utilization of antibacterial active ingredients from Pu'er tea.
    6  Properties of Sugar-contained Carrageenan and Gelatin Gel
    LI Bian-sheng LIU Bo LI Dan-dan RUAN Zheng
    2013, 29(8):1774-1778.
    [Abstract](1643) [HTML](0) [PDF 464.36 K](961)
    Abstract:
    For development of soft candy, influence of colloid concentration and syrup proportion on the textural properties and water activity of sugar-contained gel were researched. Results showed that sugar-contained carrageenan gel formed with varied hardness when, 1% to 3% of carrageenan was used. For the sugar gel containing 1% carrageenan, higher than 69.8% of total sugar content resulted in the decrease of the water activity to 0.75.For 9.5% gelatin-contained gel system, 64% of the total sugar led to 0.75 of water activity of the gel and the gel formed was elastic. In the mixed gum sugary system, along with the increase in the amount of gelatin added, the hardness of the mixed gum gel increased, and its elasticity first increased but then decreasedto a certain extent. The cohesion of the mixed gum gel gradually increased, thus making chewiness increased. The optimum content of carrageenan, total sugar and gelatin were 0.8%, 65% and 6.4%, under which the water activity reduced 0.75. It was also found that high-fructose corn syrup had higher capability in reducing the water activity than sucrose.
    7  Effect of Petroleum Eether Extracts from Fomitopsis officinalis (Vill.:Fr.) Bond. on Tumor Cell Proliferation and Cell Cycle
    LI Xiao-fei HU Shan-shan GAO Jun-jun ZHU Xin-ting LIU Yun
    2013, 29(8):1779-1783.
    [Abstract](1466) [HTML](0) [PDF 601.40 K](730)
    Abstract:
    The sulforhodamine B (SRB) assay was employed to evaluate the anti-tumor effect of petroleum ether extracts from Fomitopsis officinalis (Vill.:Fr.) Bond (PEFO) on SMCC- 7721,SGC-7901,and Hep-2 cell lines in vitro. The results showed PEFO inhibited the growth of three kinds of cells in dose-dependent manner with the IC50 values on SMCC-7721,SGC-7901,and Hep-2 cells being of 58.74,74.13 and 83.18 μg/mL, respectively. The SMCC-7721 cells exposed to PEFO obviously changed cell morphology and cellular ultrastructure. The SMCC-7721 cell presented wrinkled nuclei, poor cell adherent growth and cell lysis, while mitochondria swell and a large number of vacuoles in cytoplasm were observed by transmission electron microscope. The flow cytometry results revealed that SMCC-7721cell decreased in S-phase and increased in G0/G1-phase, which arrested in G0/G1 phase. The study demonstrated that PEFO could inhibit cell proliferation in vitro, and the anti-tumor activity of PEPO might be achieved by inhibition of tumor cellular aerobic metabolism and intervention cell cycle.
    8  Physicochemical Properties of Pumpkin Starches from Different Cultivars
    ZHOU Ai-mei YANG Hui YANG Lei LIU Xiao-juan LIU Xin YANG Gong-ming CHEN Yong-quan
    2013, 29(8):1784-1790.
    [Abstract](1551) [HTML](0) [PDF 580.37 K](752)
    Abstract:
    The physicochemical characteristics of pumpkin starches from three cultivars (Miben, Dongshen and Cuili) were comparatively studied. The results showed that crude fat contents in three pumpkin starches varied between 0.15% and 0.25%, while crude protein varied between 0.09% and 0.57%. The amylose contents changed between 26.4% and 29.0%, and soluble amylose contents ranged between 4.48% and 7.40%. Dongshen pumpkin starch showed apparently higher contents of amylose and soluble amylose than the other two pumpkin starches. Scanning electron microscopy showed that pumpkin starches consisted of oval granules at the size of 5~15 μm with an average diameter around 10 μm. X-ray diffraction studies showed that all pumpkin starches exhibited a B-type diffraction pattern and the degrees of crystallinity were 30.9%, 42.7% and 43.3%, respectively. The ranges of initial and terminated gelatinization temperature of Dongsheng and Cuili pumpkin starches were 73.21~82.76 ℃ and 78.66~89.88 ℃, respectively. While MiBen pumpkin starch had a bimodal phenomena with two gelatinization temperature ranges being of 69.00~75.67℃ and 77.70~86.17 ℃. MiBen pumpkin starch had the lowest solubility, swellability and sediment stability, but the highest paste light transmittance. Dongsheng pumpkin starch had low solubility and paste light transmittance, but moderate to good sediment stability and freeze-thaw stability. CuiLi pumpkin starch had the highest solubility and swell-ability, but showed the lowest freeze-thaw stability.
    9  In Vitro Antioxidative Activity of Germanium-rich Exopolysaccharides from Ganoderm Applanatum
    LI Zheng-peng ZHU Chang-wei WU Ping WANG Song-hua SUN Yu-jun
    2013, 29(8):1791-1795.
    [Abstract](1471) [HTML](0) [PDF 508.31 K](710)
    Abstract:
    Germanium-rich exopolysaccharides (GEPS) was prepared via submerged fermentation of Ganoderm applanatum, and the antoxidative activity of GEPS in vitro was evaluated by determining its capability in eliminating superoxide anion free radical, hydroxyl radical, DPPH?and sodium nitrite. Also, those various antioxidant activities were compared to Vc. Results showed that GEPS reached 1.12 g/L, being of 36.62% higher than those of the control. The organic Germanium reached 0.26 mg/g, being of 116.67 % higher than those of the control when the concentration of GeO2 in culture medium was 150 μg/mL. The organic Germanium could greatly enhance the free scavenging effective to superoxide anion free radical, hydroxyl radical, DPPH?, sodium nitrite and deoxidization capacity of exopolysaccharides, being of 25.52%, 31.83%, 26.95% and 16.38%, respectively, higher than those of the control when GEPS was 2.5 mg/mL. The antoxidative activity of GEPS displayed a dose-response relationship with its concentrations.
    10  Anti-browning Effect of 1 -MCP and Film Treatments on Lijiang Snow Peach During Cold Storage
    DONG Wen-ming TIAN Su-mei HE Lian-jun
    2013, 29(8):1796-1799.
    [Abstract](1357) [HTML](0) [PDF 497.87 K](744)
    Abstract:
    The ethylene receptor inhibitor 1- methylcyclopropene model (1-MCP) combined with cling film was tested for preservation of Lijiang snow peach picked from Lashi township of Yulong county, Lijiang city, at low temperature storage(3 ± 1 ℃). The results showed that at refrigerated temperature, 1-MCP treatment could inhibit the respiration of Lijiang snow peach, Inhibit the increase of cell membrane permeability and maintain the fruit weight. However it increased the POD activity of the peach. Film bag processing can keep moisture, inhibited the POD activity and cell membrane permeability. The inhibition effect of film bag on peach respiration was lower than that of 1-MCP treatment. The combination treatment of 1-MCP and film bag treatment not only maintain moisture, but inhibit the cell membrane permeability ,respiration and POD activity. In addition, the combined treatment can effectively inhibit the decrease of total phenolic content and PPO activity, thus preventing the browning of fresh Lijiang snow peach.
    11  mmunological and Antitumor Activities of the Polysaccharides from Pholiota nameko
    XIANG Ying CHEN Jian
    2013, 29(8):1800-1804.
    [Abstract](1578) [HTML](0) [PDF 488.37 K](752)
    Abstract:
    Guinea pig blood serum was chosen as the complement to study anticomplement research and mouse peritoneal macrophages RAW264.7 was used for toxicity test. The capabilities of macrophage englobing neutral red and secreting NO and H2O2 were investigated to study Pholiota nameko polysaccharides (PNP) immunocompetence. The antitumor activities of the PNP were studied through prostatic cancer 22Rv.1 cells and human hepatoma Hep 2B cells inhibition. The results showed that the PNP owned good anticomplement activity and activity of PNP was strengthened with its concentration increased.when increasing the polysaccharides to 12.5 mg/mL above. In concentration of 0.005~0.5 mg/mL, PNP could promote the proliferation of macrophages and peritoneal macrophage phagocytic ability, and increased NO and H2O2 secreting, indicating that PNP had good immunological activity. PNP had strong inhibition to the prostate cancer cells and liver cancer cells, with a concentration-response relationship.
    12  The Analysis of Electrohydrodynamic (EHD) Drying Characteristics and Construction of Mathematical Modeling of Cooked Beef
    DING Chang-jiang LV Jun
    2013, 29(8):1805-1809.
    [Abstract](1233) [HTML](0) [PDF 488.42 K](1379)
    Abstract:
    The effect of thickness and cross-sectional area of material on drying characteristics was studied and a mathematical model was developed to describe the thin layer electrohydrodynamic (EHD) drying. The drying experiment of cooked beef was investigated with the electrohydrodynamic (EHD) drying technique in ambient (ambient temperature 20~25 ℃, ambient relative humidity 30±5% and wind speed 0 m/s). The cooked beef was put into high-voltage electric field with the different thickness or cross-sectional area. Eight different mathematical drying models were compared based on root mean square error, reduced chi-square and modeling efficiency to estimate drying curves. The results showed that the thickness of cooked beef had high impact on the drying rate and time and the cross-sectional area of cooked beef had smaller effect on the drying rate and time. The Logarithmic model could sufficiently describe thin electrohydrodynamic (EHD) drying of cooked beef. This work would provide some clues and practices guidance for optimizing the drying technology and improving the drying efficiency of electrohydrodynamic (EHD) drying.
    13  Effects of High Pressure on the Gelatin Properties and Molecular Structure of Peanut Protein Isolates
    HE Xuan-hui LIU Hong-zhi LIU Li HU Hui WANG Qiang
    2013, 29(8):1810-1815.
    [Abstract](1281) [HTML](0) [PDF 723.05 K](733)
    Abstract:
    The change of heat-induced gelatin hardness, springiness and cohesiveness of PPI (5%) treated under pressure 50~200 MPa for 5 min were investigated and compared with the untreated PPI by various methods including SDS-PAGE, circular dichroism spectra, mass spectrometry and estimation analysis of molecular structure. SDS-PAGE analysis indicated a subunit known as conarachin Ⅱwhit molecular weight 61.0 ku was more sensitive to pressure, and mass spectrometry analysis shown this subunit contained 580 amino acids whose sequence was not changed after pressure treatment and its actual molecular weight was 66.5 ku. Estimation analysis of molecular structure of this unit showed its steric conformation was significantly changed after pressure 100 MPa treatment. Results from CD showed a secondary structure of PPI also greatly changed under high pressure treatment. PPI treated under 100 MPa resulted in the largest value for heat-induced gelatin hardness, increased by 49.6% compared with untreated PPI and reached 172 g, while springiness and cohesiveness remained equal to that of untreated. All the findings above indicated pressure treatment can improve heat-induced gelatin properties of PPI.
    14  An Improved Chitosan Coating Method for Bacteriostasis and Preservation of Grass Carp During Refrigerated Storage
    WANG Fa-xiang WANG Man-sheng LIU Yong-le YU Jian WANG Jian-hui LI Xiang-hong
    2013, 29(8):1816-1819.
    [Abstract](1134) [HTML](0) [PDF 365.30 K](735)
    Abstract:
    Chitosan coating is a new preservation technology with good preservation effect and superiority, but has some disadvantages, such as slow speed of film forming, difficulty in the operating and controlling the film thickness. Thus, improving the composition of coating solution and the coating technology is important for its preservation effect. The improved preservative and antibacterial effects on refrigerated grass carp meat was investigated by using new coating method with adding volatile ethanol. By varying the concentrations of ethanol and chitosan in coating solution, as well as coating methods, the total bacteria colony and TVB-N of the fish during its cold storage were studied. The results showed that the coating solution containing 75% ethanol had better antimicrobial and bactericidal effects with a decrease of >51% in initial bacterial count of fresh fish meat. The best chitosan concentration was 1.0% comprehensively considering its film-forming ability and antibacterial effect. In addition, the coating methods showed little difference in affecting the preservation of Grass carp, while spray coating pattern was relatively convenient in operation and economical in coating solution. These findings demonstrated that spray coating using acidic chitosan solution containing 75% ethanol was an effectively new fish preservation technology.
    15  Solute Penetration of Shrimp during Immersion Chilling and Freezing Process
    LIN Wan-ling YANG Xian-qing HOU Cai-ling HAO Shu-xian LI Lai-hao HU Xiao YANG Shao-ling WEI Ya CHEN Shen-jun GAN Zhi-en
    2013, 29(8):1820-1825.
    [Abstract](1250) [HTML](0) [PDF 585.46 K](786)
    Abstract:
    During immersion chilling and freezing (ICF) process, mass transfer was a major factor directly affecting the application of ICF. The penetration model and law of different solutes during ICF was investigated by establishing the gelatin models and explored the penetration law of shrimp. The results indicated that the penetration depths of betaine, propanediol and sodium chloride in gelatin model were 0.2 cm, 0.35 cm and 0.4 cm, respectively, while the penetration depths of them in shrimps were 0.25 cm, 0.31 cm and 0.35 cm, respectively. The transfer contents of betaine, propanediol and sodium chloride in gelatin were 4.35±0.12 mg/g, 6.15±0.12 mg/g and 11.73±0.18 mg/g, respectively, and the transfer contents of them in shrimps were 0.86±0.07 mg/g, 1.53±0.11 mg/g and 1.67±0.08 mg/g, respectively. Through regression analysis of solute changes during ICF, the solute penetration regression model could express the penetration law of solute. It was found that transfer trend of betaine, propanediol and sodium chloride in shrimp and gelatin were consistent. Before 5 min, the penetration rates of betaine, propanediol and sodium chloride showed a linear increase. After 6 min, the penetration rates became slow. According to the math model, the diffusion coefficients of betaine, propanediol and sodium chloride in gelatin model were 4.16×10-7 m2/s,1.27×10-6 m2/s and 1.66×10-6 m2/s,respectively. The diffusion coefficients of three solutes in shrimps were 2.34×10-7 m2/s, 9.36×10-7 m2/s and 1.36×10-6 m2/s, respectively.
    16  Enzymatic Hydrolysis of Coconut Protein and Antioxidant Activity of the Hydrolysates
    JIN Bei HUANG Mei-fang HE Zi-peng SHEN Shu-qin
    2013, 29(8):1826-1831.
    [Abstract](1405) [HTML](0) [PDF 545.47 K](769)
    Abstract:
    Coconut was crushed and defatted, giving coconut powder. Coconut protein was obtained by alkaline extraction and acid precipitation from coconut powder. Then coconut protein was hydrolyzed by four proteases (Alcalase, Neutrase, bromelin, Papain), among which Alcalase showed to be the optimal in terms of degree of hydrolysis and DPPH?scavenging rate of hydrolysates. By single factor and multi-index orthogonal design, hydrolysis conditions were optimized using degree of hydrolysis and DPPH?scavenging rate as the evaluating indexes. Hydrolysis temperature and substrate concentration showed the highest influence on DPPH?scavenging activity. The optimal conditions were substrate concentration 2% (m/V), enzyme concentration 14000 U/g, pH 10.5, temperature 50 ℃ and hydrolyzing time 7 h. Under these conditions, the protein content of hydrolysates, the scavenging rate on DPPH?and the degree of hydrolysis were 15.8 mg/mL, 89.07% and 29.16%, respectively. The antioxidant activity of coconut hydrolysates closed to that of the same concentration of glutathione, being 3.33 times higher than that of the same concentration of Vitamin C.
    17  Expression Changes of Proteins in Fermentation Broth of Aspergillus oryzae
    FENG Guang-li LIU Hong-wei XU Xi-lin LI Xiao-feng
    2013, 29(8):1832-1836.
    [Abstract](1423) [HTML](0) [PDF 563.17 K](841)
    Abstract:
    In this paper, biomass accumulation, dynamic expression of proteins in fermentation broth of Aspergillus oryzae were studied when glucose or tween 80 were used as additional carbon sources. When glucose was used as additional carbon source, biomass accumulation of A.oryzae was faster than tween 80 and reached the maximum of 31.97 g/L at 24 h, but accumulation of A.ryzae was slow when tween 80 was used, which reached the maximum of 11.15 g/L at 96 h. Total proteins in fermentation broth was higher with glucose as additional carbon source. Cell-bound proteins reached the maximum value of 5.65 mg/g at 72 h with tween 80 as additional carbon source, while cell-bound proteins reached the maximum of 1.71 mg/g at 48 h with glucose as additional carbon source. Expression of cell-bound proteins detected by SDS-PAGE within 192 h showed that cell-bound proteins increassed at the rapid growth and stationary phages, and reduced at the recession phage.which was in consistent with protein contents measured by the Lowry method.
    18  Characterization of Modified Peel Dreg Cellulose of Chinese Water Chestnut as Heavy Metal Ion Sorption Agent
    DANG Zi-jian HUANG Hui-hua
    2013, 29(8):1837-1842.
    [Abstract](1251) [HTML](0) [PDF 475.41 K](743)
    Abstract:
    In this research, carboxylic functional group was introduced into the fibre backbone of peel dreg of Chinese water chestnut by succinic anhydride modification in pyridine. The adsorption capability of the modified peel dreg on Cu2+, Cd2+ and Pb2+ was influenced by the initial metal concentration, adsorption time and solution pH value. The maximum adsorption capacities of modified peel dreg of Chinese water chestnut reached 44.80±2.68 mg/g for Cu2+ at pH 5.5, 80.73±6.25 mg/g for Pb2+ at pH 5.6 and 45.94±3.15 mg/g for Cd2+ at pH 7.8. As an chemical style, the kinetics of adsorption process was accorded with lagrange pseudo second-order model. The adsorption isothermal experimental data coincided well with Langmuir model. FT-IR analysis indicated that the carboxylation reaction took place during the modification of the Chinese water chestnut peel.
    19  Comparison of the Typing Methods of ERIC?PCR and Biochemical for Campylobacter jejuni Isolates
    ZHENG Yang-yun WU Qing-ping WU Ke-gang WU Kui ZHANG Ju-mei
    2013, 29(8):1843-1850.
    [Abstract](1485) [HTML](0) [PDF 703.58 K](774)
    Abstract:
    To establish an ERIC?PCR molecular typing method for Campylobacter jejuni genotyping, the subtyping effect of ERIC-PCR and biochemical typing methods were compared. L16 (54) orthogonal text was designed with four factors of Mg2+, dNTPs, primers, TaqDNA polymerase concentration to explore the suitable reaction conditions in a wide range. Then three factors (except the primers), template concentration and annealing temperature were further optimized in a small range for final optimization. The optimized ERIC?PCR method was used to type the 24 C. jejuni isolates. According to the results of biochemical reactions to biochemical type the strains, the ERIC-PCR molecular typing method was compared with biochemical typing method. C. jejuni strains were grouped into 22 types. The ERIC?PCR method exhibited better disciminative results in molecular typing with discrimination index of 0.92. The C. jejuni isolates were classified to 19 biochemical types by biochemical typing method, which showed the diversity of genes in the strains. The ERIC?PCR subtyping method showed the genetic diversity of the strains better than biochemical typing method, which was an efficient method for typing and tracking analyses. It was suitable for genetic diversity analysis of of C. jejuni strains.
    20  Composition and Antioxidant Activity of Proteins from Dried Litchi Fruit
    TANG Dao-bang BU Zhi-bin XU Yu-juan Wu Ji-jun WEN Jing YU Yuan-shan
    2013, 29(8):1851-1856.
    [Abstract](1403) [HTML](0) [PDF 524.47 K](771)
    Abstract:
    The composition of proteins from dried litchi fruit was analyzed and the antioxidant activity of different protein fractions were compared. Different components of proteins were extracted with isoelectric point (pI) method and amino acids of extracted proteins were measured by an automatic amino acid analyzer. A preliminary analysis of different components of small molecular proteins (SMP) were studied by ultra performance liquid chromatography-mass spectrometer Q1 scan. The best extraction time was 2 h for litchi pulp static leaching. The contents of four components with pI values of 3.5, 5.5, 6.5 and 7.5 were 25.03%, 26.98%, 1.83% and 0.53%, respectively. The litchi proteins had the same amino acid composition but different amino acid contents. Molecular weights (MW) of the mian component of SMP at pI 3.5 and 5.5 were near 5000 Da, while MW of the main components of SMP at pI 6.5 and 7.5 were near 1000 Da and 5000 Da. The litchi proteins with different pI were different in antioxidant activity, amino acid contents, MW distribution and antioxidant activity.
    21  Separation and Characteristics of Raffinose Lauric Acid Monoester
    LU Yu-yun YAN Ri-an MA Xiang WANG Yong
    2013, 29(8):1857-1861.
    [Abstract](1397) [HTML](0) [PDF 596.56 K](759)
    Abstract:
    Raffinose long-chain fatty acid monoester, acylated at the 1”-and 6”- positions, was synthesized in dimethylsulfoxide by the acylation of raffinose with methyl laurate under reduced pressure with ultrasound irradiation. TLC analysis of 4μL reaction mixture were carried out on silica gel G plates using chloroform-methanol-aceticacid (V/V/V, 75:25:7) as eluent. Spots were located by spraying with 10% of phosphomolybdic acid hydrate in ethanol and heated at 105 ℃ for 10min. The product was then separated with silica gel column chromatography by dissolving 1 g sample in eluent and then eluted by chloroform-methanol-acetic acid on a 15 mm × 700 mm silica gel column (200~300 mesh) at flow rate of 1 mL/min. The eluent of 1 bottle/10 min was collected and the purified products were analyzed by HPLC, IR, MS and NMR, and identified as 1’’-O-lauroylraffinose and 6’’-O-lauroylraffinose. The two lauroylraffinose isomerides had similar solubility in polar solvent and high thermal stability.
    22  Antibacterial Activity and Mechanism of Action of 10-HDA against Bacillus subtilis
    WANG Rui-ming LI Jun-lin YANG Xiao-hui WANG Teng-fei WANG Jian-bin
    2013, 29(8):1862-1866.
    [Abstract](1547) [HTML](0) [PDF 514.86 K](975)
    Abstract:
    Through oxford cup test and half dilution method for testing antibacterial activity, the preparation progress and antibacterial activity of 10-HDA against Bacillus subtilis were identified and analyzed in this paper. The quantity and bonding of bacteria DNA affected by 10-HDA were analyzed by using atomic force microscope (AFM) and agarose gel electrophoresis of DNA. The results showed that, 10-HDA was a broad-spectrum antimicrobial agent and had obvious inhibition effects on multiple pathogenic bacteria with a concentration-depended mode of inhibitory effect. The minimum inhibitory concentration (MIC) of Bacillus subtilis was 0.62 mg/mL. Agarose gel electrophoresis and AFM analyses revealed that the synthesis of DNA was rapidly decreased due to the close combination with 10-HDA. It was further proved by its impact on PCR of DNA that the synthesis of DNA could be completely impeded when the mixing concentration of 10-HDA was 1.0mg/ml. Therefore, bacteriostatic action was inferred to be caused by the combination between 10-HDA and DNA which inhibited the replication of DNA.
    23  Biotransformation of Soybean Isoflavone by Microbial Solid-state Fermentation
    YANG Shou-feng XU Jian-xiong
    2013, 29(8):1867-1871.
    [Abstract](1353) [HTML](0) [PDF 638.66 K](946)
    Abstract:
    Soybean insoflavones (SIF) are rich in nutritional value and heathy benefit, which had been widely applied in various fields including food, feed, medicine and so on. To extend the application of SIF for industrialization production, the efficacy of biotransformation of SIF by microbial solid-state fermentation was studied using EM strains, Bacillus subtilis ATCC 6633 and Lactobacillus planetarium ATCC 8014 for single and mixed fermentation. Effects of combined strains and incubation size were investigated for improve the biotransformation efficiency of SIF through vacuum sealed fermentation. The repeatability of the samples was also investigated under the optimized fermentation conditions. The results demonstrated that the single strain-mediated fermentation resulted in higher biotransformation efficacy than that with mixed strains, and the ratio of aglycone in total SIF content reached 64.56% after 7 d by solid-state fermentation with Bacillus subtilis ATCC 6633. The fermentation had high repeatability and stability.
    24  Taste Compounds in Maillard Reaction Products of Chicken Bone Extract and its Enzymatic Hydrolysate
    SUN Hong-mei ZHANG Chun-hui LI Xia LI Yin DONG Xian-bing WANG Chun-qing XIE Xiao-lei
    2013, 29(8):1872-1877.
    [Abstract](1319) [HTML](0) [PDF 643.26 K](833)
    Abstract:
    In this paper, the taste characteristics of Maillard reaction products prepared by chicken bone extract (MRPs1) or its enzymatic hydrolysate (MRPs2) was investigated. The sensory evaluation was used to analyze the taste of the product as a qualitative analysis. The non-volatile taste active compounds, including free amino acids, oligopeptide, total nucleotide, flavor nucleotide in the Maillard reaction products were analyzed by quantitative analysis, combining monosodium glutamate equivalent (EUC) to assess the umami of MRPs. The results showed that total free amino acid content of MRPs1 was 1.4 mg/g and MRPs2 was 8.1 mg/g. The content of AMP, GMP and IMP were 67.83, 176.52 and 415.60 μg/mL, respectively. In MRPs2, the total content of the three amino acids increased by 32.83%. Showing different contribution to taste of the product . In addition, the EUC of MRPs2 were significantly higher than that of MRPs1, which was confirmed by the Electronic Tongue detection..
    25  Release Properties of Glutamine Peptide from Different Wheat Gluten Sources
    WANG Yan-zhou LIU Li-ya ZHONG Kui TONG Li-tao SUN Meng-ying ZHOU Su-mei
    2013, 29(8):1878-1882.
    [Abstract](1349) [HTML](0) [PDF 596.69 K](886)
    Abstract:
    12 kinds of commercial wheat gluten were collected from the domestic wheat gluten manufacturers. Based on the analysis of the basic components, the enzymatic hydrolysis and glutamine peptide release were studied. The results showed that average protein content of these 12 samples was 77.6%, and there were little differences in protein level between these samples, with the coefficient of variation (CV) of 2.7%. Significant difference in starch contents was found with CV value of 21.65%. The fat content was very low with an average value of 1.1%, but was evidently varied in different samples tested (CV: 69.6%). Analysis of amino acid composition of different wheat gluten samples showe that glutamate/glutamine content was 412.58 mg/g protein with their proportion up to 39.38%. Considering protein recovery, degree of hydrolysis and glutamine peptide content, No.1 (Binzhou, Shandong), No.9 (Baoji, Shanxi) and No.12 (Xingtai, Hebei) were chosen as ideal raw materials for preparation of glutamine peptide. In the hydrolysates of the three samples, the highest glutamine content, degree of hydrolysis and protein recovery were up to 22%, >15% and > 90%, respectively.
    26  Effect of Nano-chitosan Coating on Quality of Fresh-cut Zizania latifolia
    ZHOU Jing-feng LUO Hai-bo WANG Jun JIANG Kai YU Zhi-fang
    2013, 29(8):1883-1887.
    [Abstract](1478) [HTML](0) [PDF 521.06 K](725)
    Abstract:
    The effect of nano-chitosan coating on the quality of fresh-cut Z. latifolia was investigated. Fresh-cut Z. latifolia slices were dipped into deionised water (the control), 10 g/L chitosan or the mixture of 10 g/L chitosan and 1.5 g/L nano-chitosan for 3 min, and then dried, packaged and finally stored for 12 days at 1±0.5 ℃. Changes in whiteness index (WI), firmness, weight loss, lignin, reducing sugar and ascorbic acid contents, phenylalanine ammonia-lyase (PAL), superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities were evaluated at intervals of two days. The results showed that the nano-chitosan coating significantly inhibited the decline of WI value and the increase of lignin content, maintained relatively high firmness, reducing sugars and ascorbic acid contents of fresh-cut Z. latifolia. After a 12-day storage at 1±0.5 ℃, the weight loss of the fresh-cut Z. latifolia slices was 1.12%. The treatment also inhibited the activities of PAL and POD but promoted SOD and CAT activities compared with the control. The present findings indicated that the nano-chitosan coating could inhibit browning and lignifacation and improve antioxidant enzymes activities, which suggested that the nano-chitosan coating had potential commercial value in controlling quality deterioration of fresh-cut Z. latifolia.
    27  A Mutant Strain with High Threonine Yield Obtained by Rapid Mutation Using Novel Atmospheric and Room Temperature Plasmas
    CAI You-hua LI Wen-feng LU Wei-ning ZHENG Ming-ying LU Zui-qing ZHANG Chong LI He-ping XING Xin-hui
    2013, 29(8):1888-1892.
    [Abstract](1714) [HTML](0) [PDF 590.56 K](852)
    Abstract:
    To obtain mutants with high threonine productivity, atmospheric and room temperature plasmas (ARTP) mutation technique was used and combined with high throughput screening based on 48 well plates. Mutant 1905# was screened with high threoine productivity. Compared with original strain under the same fermentation conditions, the mutant 1905# showed better robustness and faster growth rate. The mutant showed similar optimum temperature (37 ℃) and pH (7.2) to the original strain, but it can tolerate lower pH. In the preliminary plate screening, the threonine concentration increased by 133.59%. For the flask fermentation process, the mutant 1905# showed higher glucose consumption and enhanced threonine productivity The threonine concentration by the mutant was increased by 99.6% compared with that by the original. The 1905# mutant showed high genetic stability after 50 subcultures, offering theoretical reference for further increasing threonine industrialization level
    28  Effects of 1-MCP Combined Chitosan on Storage Quality of Fresh-cut Potato
    LI Ling GUO Yan-yin
    2013, 29(8):1893-1897.
    [Abstract](1136) [HTML](0) [PDF 670.44 K](744)
    Abstract:
    The effects of 1-methylcyclopropene (1-MCP) combined chitosan on storage quality of fresh-cut potato during storage were investigated by treatment of the fresh-cut potato with the mixture of 1-MCP (2 μL/L) and chitosan (0.1~1.5% ) and using fresh-cut potato without any reagent as check treatment (CK). The mixture of 1-MCP and 1.5% chitosan (1-MCP+1.5% chitosan treatment) showed to be the most effective treatment during the whole storage, followed by 1-MCP+1.0% chitosan, 1-MCP+1.0% chitosan, 1-MCP ,1-MCP+0.5% chitosan and1-MCP+0.1% chitosan. 1-MCP+1.5% chitosan treatment could maintain high activities of SOD and POD, delay the accumulation of MDA and decrease the Vc content and inhibit the activity of PPO and browning of fresh-cut potato, thus significantly maintain the storage quality of fresh-cut potato.
    29  Inbibitional Effect of Sandy Tea on the Carcinoma Cells Growth and Tumor Metastasis
    FENG Xia LUO Min ZHAO Xin
    2013, 29(8):1898-1901.
    [Abstract](1328) [HTML](0) [PDF 505.95 K](791)
    Abstract:
    In this study, a sandy tea product was purchased for the evaluation of in vitro anticancer effect on TCA8113 human tongue squamous carcinoma cells and in vivo anti-metastatic effect.By using MTT assay, apoptosis analysis by DAPI staining and RT-PCR assay, and western blot assay were employed to check the in vitro anticancer effect and in vivo anti-metastatic effect of Sandy tea at different concentration (50, 100 and 200 μg/mL) using animal models. Results showed that 200 μg/mL of sandy tea (81%) had the best inhibitory effect and apoptosis-inducing activity on TCA8113 human tongue squamous carcinoma cells compared to those samples at other concentrations.With increasing the tea concentrations, mRNA expressions of Bax, caspase-3 and caspase-9 was up-regulated, but Bcl-2 expression was down-regulated. BALB/c tumor anti-metastasis test showed that sandy tea inhibited in vivo tumor metastasis induced by colon 26-M3.1 cells in BALB/c mice. These results suggest that sandy tea had potential as an anticancer functional food.
    30  Frost Resistance of Ten-Degree Refined Palm Oil
    LI Qiang CAO Wei-jin LIU Li-ping XIONG Yue-wen
    2013, 29(8):1902-1905.
    [Abstract](1227) [HTML](0) [PDF 433.37 K](755)
    Abstract:
    Ten-degree palm oil was produced by fractionation and physically refination to reach the product quality requirements. But the frost resistance of refined ten degree palm oil was worse, thus affecting product transparency. In order to reduce the effect of physical refined conditions on the frost resistance of refined ten degree palm oil, turbid phenomenon at 20 ℃ was used as a judicial indicator and the effects of deodorized temperature, deodorized time, bleached time and activated clay loading were investigated. The results showed that deodorized temperature, deodorized time and activated clay loading had effects on the frost resistance of refined ten degree palm oil, while bleached time had little effect on it. By comparing the frost resistance, iodine value, trans fatty acids of unrefined and refined ten degree palm oil,the optimal physical refined conditions were determined as follows: bleached time 20 min, activated clay loading 1%, deodorized temperature 230 ℃ and deodorized time 2 h.
    31  Preparation and Characterization of Functionalized Nano-Al2O3/PU Sol
    XIAO Kai-jun TANG Xu LIAO Wei
    2013, 29(8):1906-1910.
    [Abstract](1333) [HTML](0) [PDF 508.50 K](808)
    Abstract:
    The functionalized nano-Al2O3/PU sol was prepared in a sol-gel process, using aluminium isopropoxide (AIP) as raw material and polyurethane (PU) as a coupling agent. The effects of hydrolysis temperature, n(H2O)/n(Al3+), n(H+)/n(Al3+), peptization agent, aging temperature and time on the particle size and viscosity of the sol were studied. The optimal conditions for preparing stable and transparent functionalized nano-Al2O3 sol were obtained as hydrolysis temperature 80 ℃, n(H2O)/n(Al3+) 105, n(H+)/n(Al3+) 0.24, HNO3 as peptization agent, aging temperature 80 ℃ and aging time 24 h. The properties and structure of the sol were characterized by Nano particle analyzer and FI-IR. The results indicated that the particle size and viscosity of Al2O3/PU sol ranged from 40 to 120 nm and 4 to 10 mPa?s, respectively. C=C double bond and the hydrophilic groups, such as hydroxyl group and carboxyl group were successfully introduced to the functionalized nano-Al2O3/PU sol, which can enhance the chemical bond between AIP and PU and increase the strength of the sol.
    32  Effect of Roasted Starch on Soy Sauce Koji-making
    CUI Chun OU Yang-shan YIN Wen-ying REN Jiao-yan ZHAO Hai-feng ZHAO Mou-ming
    2013, 29(8):1911-1915.
    [Abstract](1453) [HTML](0) [PDF 573.12 K](821)
    Abstract:
    In this study, wheat and flour were chosen as raw starch materials to investigate the effect of roasting on soy sauce koji-making. Indexes including activities of protease, aminopeptidase, amylaseand cellulose enzyme, NSI and other physiochemical indexes were compared between kojis of the roasted wheat and original wheat, as well as between that of the roasted flour and original flour. All the compassions were conducted under the optimum roasting time, which was determined by conversion rate of total sugars and protein recovery of kojis hydrolyzates. The optimum roasting time for both wheat and flour was 20 min, under which the enzyme systems and physiochemical indexes of kojis were well influenced. The activities of protease, aminopeptidase and cellulose enzyme, spore and NSI of koji gained from roasted wheat increased by 13.06%, 12.58%, 9.92%, 37.04% and10.46%, respectively when compared with that from original wheat. The activities of protease, aminopeptidase, cellulose enzyme, spore and NSI of koji gained from roasted flour were increased by 32.88%, 28.17%, 18.07%, 13.04% and 10.79%, respectively. The amylase activities of kojis cultivated with roasted wheat and flour both decreased a little. In conclusion, the use of both the roasted wheat and flour can improve the quality of kojis, thus starch material roasting can be an alternative way to improve the enzyme systems and physiochemical indexes of kojis.
    33  Effect of Different Drying Technologies on Qualities of Jinmudan Oolong Tea
    ZHANG Ling-yun WEI Qing WU Ying DAI Yong-feng CUI Fei-long
    2013, 29(8):1916-1920.
    [Abstract](1440) [HTML](0) [PDF 512.79 K](761)
    Abstract:
    In this study, traditional drying, multiple drying and microwave drying were applyed to Jinmudan Oolong tea drying. Biochemical components and aroma compositions of the tea samples were different when different drying technologies were employed. The use of microwave drying gave lower contents of tea polyphenols and soluble sugar but higher content of amino acid that the traditional method. The aroma components were extracted by SDE and analyzed by GC-MS. It was found that types of tea aroma components in microwave drying (41 kinds) were less than those by the traditional drying (48 kinds). The main aroma components of Jin Guan-yin oolong tea included nerolidol, methyl linolenate, alpha-afarnesene, indole, 5 - hydroxy decanoic acid lactone, beta - benzene ethanol, palmitic acid, phytol, phenyl acetaldehyde, and cis-jasmone. Nerolidol was shown to be the aroma components with the highest content in Jin Guan-yin oolong tea, which accounted for 39.72%, 35.68% and 43.9%. Although the traditional drying method can well maintain the quality of Jinmudan oolong tea, microwave drying was more convenient for practical processing and also resulted in good quality of Jinmudan oolong tea .
    34  Comparison and Dynamic Models of Supercritical CO2 Extraction Enhanced by Different Technologies
    LUO Deng-lin XU Bao-cheng QIU Tai-qiu LIUJian-xue
    2013, 29(8):1921-1925.
    [Abstract](1096) [HTML](0) [PDF 587.58 K](765)
    Abstract:
    In order to extend the scope and improve the efficiency of supercritical CO2 extraction, four enhanced technologies including supercritical CO2 extraction with modifier (SCE), supercritical CO2 extraction with ultrasound combined modifier(USCE), supercritical CO2 reverse microemulsion extraction (SCRME) and supercritical CO2 reverse microemulsion extraction with ultrasound(USCRME) were applied to extract ginsenosides from ginseng. Their extracting kinetic models were built based on transfer theory and principle of mass balance. The results showed that the extraction yields of ginsensides by SCE, USCE, SCRME and USCRME were 0.26%, 0.45%, 0.76% and 1.15%, respectively. The extraction rates of ginsensides by four methods showed similar order to the extraction yield. The fitting degree of the model and the experimental data was more than 99.5%. The maximum E∞ (1.213%) and k (0.659) values were obtained by USCRME model simulation, which were 3.95 and 1.32 times, respectively, of those by SCE. The results demonstrated that supercritical CO2 extraction enhanced by ultransound combined with reverse microemulsion could improve the extraction yield and rate of polar material ginsensides.
    35  Increasing the Acid Yield Rate of Solid-state Vinegar Fermentation Based on Temperature Online Monitoring Technology
    ZHU Yao-di ZOU Xiao-bo LIN Hao SHI Ji-yong XIA Rong
    2013, 29(8):1926-1930.
    [Abstract](1231) [HTML](0) [PDF 567.21 K](823)
    Abstract:
    Zhenjiang aromatic vinegar is one of the most famous Chinese traditional vinegar, whose traditional process depends on the subjective experience of workers to determine the time and frequency of stirring culture. It is difficult to make scientific adjustment according to the season changes, resulting in large different of the acid production rate. Especially in summer, the acid production rate is very low. In order to overcome this problem, an intelligent acquisition module based on thermal resistance was build and applied to real-time monitor the temperature of vinegar culture in the fermentation process. The temperature of the heating stage was controlled by continual stirring the vinegar culture without changing traditional process to improve the acid production rate. At the same time, the pH value, total acid content and activity of ethanol dehydrogenase at different stages of fermentation were detected. The results showed that the activity of ethanol dehydrogenase in temperature-adjusted group was higher than that in control group, indicating that suitable fermetnation temperature can enhance the metabolic activity of acetic acid bacteria. And the acid production rate was increased by 1% based on the temperature online monitoring technology. The new method of the stirring culture can eliminate effectively the phenomenon of changes in the acid production rate due to changes in season. This study showed that controlling the temperature of the heating stage was very effective to increase the acid production rate.
    36  Effects of Hsian-tsao Extracts on Quality of Low-temperature-cooked Beef Roll
    LI Jun-guang ZHOU Quan JIANG Ai-min YIN Bei-bei WANG Zhi-jiang
    2013, 29(8):1931-1937.
    [Abstract](1270) [HTML](0) [PDF 605.70 K](670)
    Abstract:
    The effect of a new plant gel extracted from Hsian-leaf mixed with other food binders on texture profile and sensory properties of cooked beef roll was investigate. The result revealed that the extracts of hsian-tsao could enhance the function of carrageenan, soyprotein isolate, and potato starch to improve the quality of cooked beef roll efficiently. Response surface model (RSM) analysis indicated that the sensory scores of the beef roll increased with the concentration of hsian-tsao extracts and potato starch usage up to an optimum level, and then decreased. The sensory scores increased with soy protein isolate, but the change tended to be slowly. The sensory properties were significantly affected by hisan-tsao extracts. Hardness of the beef roll increased with increasing usage of soy protein, potato starch and hisan-tsao extracts, but then decreased when futher increasing their dosages. The optimized formula for 100 g beef was determined as follows: 50 mL hsian-tsao leaf extracts w diluted with water by 1:20.6, 5.13 g potato starch, 5.12 g ISP, 2 g CG, 2.8 g salt,1g cane sugar, 0.04 g sodium ascorbate, 0.004 g sodium nitrite, 0.1 g TSPP, 0.125 g STPP, 0.075 g HMP, and 0.003 g monascus red pigment. The product made under these optimum conditions showed the sensory scores of 8.15 points, hardness of 3381.1g and chewiness of 3373.94g.
    37  Co-immobilized Glycerol and Zygosaccharomyces rouxii Used in the Fermentation of High Salt Diluted-state Soy Sauce
    WU Hui-ling WEI Lu-ning GAO Xiang-yang PAN Hai-peng YUAN Guo-xin YAN Zhe ZHOU Zi-qi HU Wen-feng
    2013, 29(8):1938-1942.
    [Abstract](1439) [HTML](0) [PDF 506.48 K](768)
    Abstract:
    The protective effect of glycerol, one of the compatible solutes, on Zygosaccharomyces rouxii in high osmotic pressure condition was studied and the growth of Z. rouxii was observed in 3 mol/L NaCl YPD mediums with different concentrations of glycerol. Z. rouxii and glycerol were co-immobilized and applied to the fermentation of high salt diluted-state soy sauce mash. The results indicated that, 1.37 mmol/L glycerol can relieve the inhibitory effect caused by high salinity on the growth of Z. rouxii efficiently, the lag phase in growth curve of Z. rouxii was shorten by 4~6 hours. Compared with the control group, the protein conversion rate of pilot-scale fermentation of soy sauce was increased by 2.23~5.64%, and the fermentation period of soy sauce was shorten by 15 days on average. It was concluded that glycerol could protect Z. rouxii under the high-salt conditions, and improve the metabolism rate of Z. rouxii in the fermentation of high salt diluted-state soy sauce mash. Co-immobilized glycerol with yeast can improve the utilization rate of raw materials and shorten the fermentation period, showing practical application potencial in soy sauce brewing.
    38  Screening and Identification of Lactic Acid Bacteria with Inhibition Activity against Food-borne Spoilage Yeast
    RAO Yu CHANG Wei XIANG Wen-liang CHE Zhen-ming
    2013, 29(8):1943-1947.
    [Abstract](1138) [HTML](0) [PDF 526.66 K](742)
    Abstract:
    Lactic acid bacteria (LAB) with inhibition activity against food-borne spoilage yeast were screened, characterized and identified by molecular biological method. Fifty-seven LAB strains were isolated from different food products such as pickles, pickled mustards, yoghourt and hams. Overlay method showed that six LAB strains represented inhibition activity against food-borne spoilage yeast such as Candida, Pichia, Saccharomyces, Rhodotorula and Yarrowia lipolytica. Among them, strain AT6 could inhibit the growth of all eleven spoilage yeast indicators. 96 well-plate method showed that the inhibition rate of supernatant AT6 reached above 73.04%. The supernatant AT6 was not sensitive to proteinase but lose the activity at higher pH value. The inhibition activity of AT6 supernatant against Canidia albicans was maximal after 30 h incubation at 30 ℃. The genome of AT6 was extracted and the gene of 16S rRNA was amplified by PCR. The PCR products were cloned into pMD 19-T T-vector and sequenced. The sequence was BLISA and strain AT6 was identified as Weissella cibaria.
    39  Comparison of Different Methods for Total Bacteria RNA extraction
    ZOU Xiao-lei LIU Li-cui LUO Li-xin
    2013, 29(8):1948-1954.
    [Abstract](3984) [HTML](0) [PDF 553.95 K](2163)
    Abstract:
    In this research, RNA was isolated from three bacterial strains, (Escherichia coli, Bacillus subtilis and Lactococcuslactis) by using modified and un-modified Trizol and RNAiso plus methods, Triton X-100 method and Qiagen method. The quality and integrity of the extracted RNA samples were determined with agarose gel electrophoresis, nucleic acid detector and RT-PCR detection. The results demonstrate that un-modified RNAiso plus method can effectively collect high-quality RNA from the three bacterial strains. Qiagen method and un-modified Trizol method showed good efficiency when isolating total RNA from E.coli but other strains. In addition, the RNA samples also contain DNA contamination. L. lactis was more suitable for using Triton X-100 method. With the same principle, modified Trizol method, modified RNAiso plus method and Triton X-100 method reported here can be employed for extraction of RNA that are free from 16S and 23S rRNA and provide simple, rapid and effective tools for the isolation of high-quality RNA appropriate for downstream molecular experiments
    40  Changes in Amino Acid Contents of Shuangjianyuhebao Litchi during Different Storage Conditions
    CHEN Zhuo-hui HU Zhuo-yan LV En-li ZHAO Lei LI Ya-ping XU Bu-qian
    2013, 29(8):1955-1960.
    [Abstract](1217) [HTML](0) [PDF 542.20 K](880)
    Abstract:
    Changes in contents of amino acids of litchi (Litchi chinensis Sonn.) cultivar, Shuangjianyuhebao stored by refrigerated storage at 3~5 ℃ and controlled atmosphere (CA) storage (3~5 vol% O2 , 3~5 vol% CO2 at 3~5 ℃ and 85~95% RH) for 42 days were analyzed by using an automatic amino acid analyzer. The results showed that the fresh litchi has total amino acid content of 2.93 mg/g with γ-amino-n-butyric acid (GABA), alanine and glutamine being of the dominant amino acid composition. No significant difference (p>0.05) in total amino acid content between samples with refrigerated storage and CA storage was found in the early storage period. After a 42-day storage, total amino acid contents of the samples by refrigerated storage and CA storage were 2.93 mg/g and 3.13 mg/g, respectively. GABA in samples stored for 14 days by refrigerated and CA storage was increased from 1.01mg/g and 1.08 mg/g to 1.25 mg/g and 1.30 mg/g, respectively. Then GABA was decreased during refrigerated storage, but maintained a stable level during CA storage (p<0.05). Alanine content in sample stored by refrigerated and CA storage showed a little increase (p>0.05). During 7-day storages under different conditions, glutamine content was decreased and glutamic acid content was increased (p<0.05).
    41  Extraction of Volatile Oil from Tangerine Peel by Supercritical Carbon Dioxide (SC-CO2) and Analysis of Its Chemical Constituents
    HUANG Jing-sheng ZHANG Shuai LIU Fei LIU Shuo YANG Yi-ting WU Biao LIU You-hao CAO Yong
    2013, 29(8):1961-1966.
    [Abstract](1528) [HTML](0) [PDF 525.60 K](854)
    Abstract:
    Process conditions of extracting volatile oil from tangerine peel were studied by supercritical carbon dioxide (SC-CO2), and chemical constituents of the oil were analyzed by gas chromatography coupled with a mass spectrometer (GC–MS). The effects of extraction temperature, pressure, time, and particle size of tangerine peel on extraction yield of volatile oil from tangerine peel were investigated by single factor experiments. Based on the results of the single factor experiments, orthogonal test of extracting the oil was carried out. Extraction pressure and extraction time as highly significant influencing factors, and extraction temperature as significant influencing factor on extraction yield of the oil were confirmed by analysis of variance (ANOVA). In addition, the optimal conditions were determined by orthogonal test as follows: extraction temperature 35 ℃, extraction pressure 16 MPa, extraction time 120 min, and particle size of tangerine peel 30 mesh. Under the above conditions, extraction yield of volatile oil from tangerine peel was 3.96 % and polymethoxyflavones content of the oil was 33.6 %. GC–MS analysis revealed that the main three constituents of the oil were 9,12-Octadecadienoic acid (Z,Z)-methyl ester (31.90 %), 9-Octadecenoic acid-methyl ester(E) (24.52 %), and 9,12,15-Octadecatrienoic acid-methyl ester (17.53 %).
    42  Production of Fructooligosaccharides using Free-whole-cell Biotransformation by Aspergillus niger CGMCC No. 6640
    ZHOU Kang LIU Dong-mei FAN Meng-ke YE Jia-lun
    2013, 29(8):1967-1972.
    [Abstract](1346) [HTML](0) [PDF 550.18 K](821)
    Abstract:
    Fructooligosaccharides (FOS) have received particular interest because of their excellent biological and functional properties for using as a prebiotic compound. The strain Aspergillus niger CGMCC No. 6640 with independent intellectual property rights was found capable of producing FOS using sucrose as the substrate. To produce FOS using free-whole-cell biotransformation by A. niger 6640, the effects of production parameters on the biotransformation of sucrose were investigated by HPLC with the column of Rezex RCM-Monosaccharife Cat. The catalysate concentration of the Nystose (or 1F-Fructofuranosylnystose), kestose, sucrose, glucose and fructose were simultaneously detected, and their retention time was 8.403 min, 8.853 min, 9.705 min, 11.473 min and 14.683 min, respectively. The free-whole-cell concentration and substrate concentration positively affected the maximum FOS yield. However CaCl2 concentration negatively affected the FOS yield. The free-whole-cell concentration, the initial reaction pH, the temperature for catalysis, biotransformation time and sucrose concentration were 60 g/L, 7.0, 33 ℃, 40 h and 600 g/L, respectively. Under the optimal conditions were as follows, the FOS contents reached 314.60 g/L. In summary, the free-whole-cell biocatalyst of A. niger 6640 can effectively produce FOS indicating a potential for industrial production.
    43  Microwave-assisted Extraction of Yellow Pigments from Mango Peel
    YAO Zhuang-he
    2013, 29(8):1973-1977.
    [Abstract](1367) [HTML](0) [PDF 506.92 K](755)
    Abstract:
    Effects of microwave power, irradiation time and the ratio of pulp to ethanol (95% (V/V)) on microwave assistant extraction of yellow pigments in mango peel were investigated, and the optimum conditions of aforementioned factors were optimized by using orthogonal experiment. The qualitative analysis of the crude pigments obtained was carried out by color reaction. The pigments were separated and structurally identified through HPLC. The category and content of the pigments were also determined. The results showed that microwave power, irradiation time and the ratio of pulp to ethanol [95% (V/V)] significantly affected the extraction of yellow pigments in mango peel. The extraction rate and yield could be up to 89.92% (m/m) and 5.1% (m/m) respectively when the mango peel were extracted three times in 6-times ethanol [95% (V/V)] at 640 W of microwave power for 30 s. The significance order of above three factors on the extraction was the ratio of mango peel to 95% (V/V) ethanol >microwave power > irradiation time. Determination of elementary structure of pigments showed that the pigments were of carotenoid compounds, and the main components were xanthophylls with hydroxy. The total content of those components was about 99.82% (m/m).
    44  Development of Real-time RT-PCR using MGB Fluorescence Probe for Differentiation of Wild-type and Vaccine Strains of Classical Swine Fever Virus
    YU Yi-gang HUANG Yun TAO Wen-yang ZHANG Li WU Hui XIAO Xing-long LAI Fu-rao YANG Xi-hong
    2013, 29(8):1978-1983.
    [Abstract](1165) [HTML](0) [PDF 510.81 K](734)
    Abstract:
    In order to the develop multiples real-time PCR for detection and differentiation of wild-type and vaccine strains of classical swine fever virus, a pair of common primers and two differently-labeled specific MGB Fluorescence probes were designed against conserved domain of the 5’-UTR of wild-type viruses and C-strain vaccine. Moreover, in order to prevent the false negative result, pseudovirus was prepared for monitoring. The multiples real-time PCR method showed 100% specificity for the selected panel with the sensitivity being of 1 TCID50/mL for wild-type virus and 0.1 TCID 50/mL for C-strain vaccine. The reproducibility experiments showed that the variation coefficients (%CV) of intra-assay and inter-assay ranged from 0.7% to 2.2%. By using this method, CSFV RNA was detected in specimens of meat, spleen and blood with positive rates of 66.7%, 60.0% and 77.8%,respectively, while the positive rates obtained by using the standard assay was 52.4%, 40.0% and 50.0%, respectively. The result statistics showed the existence of PCR inhibitos in the clinical specimens and specimens with inhibition ratio being of 5.6~10.0%, indicating the importance of pseudovirus as IAC when PCR was used to detect the RNA of wild-type viruses and C-strain vaccine.
    45  Quality Evaluation of Star Anise Based on GC/MS Fingerprints
    WANG Qin ZHENG Xi-kang JIANG Lin LIUYao-rui WEN Qi-biao
    2013, 29(8):1984-1988.
    [Abstract](1239) [HTML](0) [PDF 507.01 K](805)
    Abstract:
    Star anise (Illicium verum Hook. f. ) is homology of medicine and food, and its essential oil extracted from fruit is not only applied in food and cosmetic, but also exerts antibacterial and antioxidant activities. In this paper, the MS fingerprints of star anise was established. Nine GC chromatographic peaks were identified as α-pinene (peak 1), limonene (peak 2), linalool (peak 3), estragole (peak 4), anisaldehyde (peak 5), trans-anethole (peak 6), bergapten (peak 7), caryophyllene (peak 8) and farnesol (peak 9),, which were as characteristic peaks in the mutual mode using trans-anethole (peak 6) as reference. By utilizing the fingerprinting, the quality of star anise harvested from 15 regions was investigated and the positive correlativity was fond between the similarity of GC/MS chromatogram and the quality of star anise. This provides guidance to develop quality evaluation method of star anise.
    46  Multidimensional Analysis of Honey Samples with Excessive Amounts of C-4 Plant Sugar
    LUO Hai-ying LUO Dong-hui XIAN Yan-ping HOU Xiang-chang GUO Xin-dong WU Yu-luan DONG Hao
    2013, 29(8):1989-1993.
    [Abstract](1513) [HTML](0) [PDF 625.51 K](821)
    Abstract:
    In this paper, 21 honey samples with excessive amounts of C-4 plant sugar were chosen from 120 specimens obtained from food market.Element analyzer-isotope ratio mass spectrometry (EA-IRMS), liquid chromatography-isotope ratio mass spectrometry (LC-IRMS) and liquid chromatography (LC) were adopted to detect the δ13C values and the amount of C-4 plant sugar. The results indicated that the honey samples with excessive amounts of C-4 plant sugar still occupy a certain proportion in food market and the over-standard rate of this batch was 16.94%. Several samples were detected with serious excessive C-4 plant sugar, one of which haDs the highest amount of 82.35%. The honey samples with excessive C-4 plant sugar can be summarized as the following three categories. (1) Samples with slightly excessive C-4 plant sugar: the standard-exceeding rate of those samples was 7~10% , and their △δ13CF-G and △δ13Cmax values were not far from the requirements of the real honey. (2) Samples adulterated with starch products of C-4 plant source: these samples had a high amount of oligosaccharide and larger △δ13Cma. (3) Samples adulterated with high fructose corn syrup; no oligosaccharide was found in these samples and their δ13CF and δ13CG completely lost the characteristics of C-3 plant.
    47  Bioinformatic Analysis of Genes Relative to the Carcinogenic Toxicity of Aflatoxin B1
    LI Li CAO Yi-cheng OU Jing-shen MAO Xiao-fan
    2013, 29(8):1994-1998.
    [Abstract](1115) [HTML](0) [PDF 415.46 K](756)
    Abstract:
    Aflatoxin is one of the most harmful mycotoxins existing extensively in daily food, among which carcinogenic toxicity of aflatoxin B1(AFB1) showed the highest carcinogenic toxicity. To screen genes strongly associated with the carcinogenic effects of AFB1, three AFB1-related human genome microarray datasets were downloaded from GEO database and analyzed by clustering and bioinformatic retrieving in this study.A set of genes were selected for clustering, which expression differences were greater than 2 fold in three microarray datasets. Then the genes expressed in accordant trend in the cluster analysis were screened and the genomic information about those genes and the association with AFB1 were determinated. Several genes such as KRT15, PCNA and MMP1 were identified as key AFB1-related genes through bioinformatics analysis on three relative microarray datasets,. These data may be benefit for further investigations of the genesis and progression of the AFB1-induced cancer.
    48  Identification of Antioxidants in Methanol Extracts from Soymilk
    ZHANG Hua SUMNER Lloyd LEI Zhen-tian HUHMAN David WATSON Bonnie FU Fu-you YU Jie
    2013, 29(8):1999-2003.
    [Abstract](1148) [HTML](0) [PDF 610.47 K](729)
    Abstract:
    The paper mainly investigated the antioxidaative capacity of the soymilk methanol extracts and analyzed the possible existing antioxidants. DPPH (1,1-Diphenyl-2-picrylhydrazyl radical) method and FRAP (Ferric reducing antioxidant power ) method were used to analyze the antioxidative capacity of soymilk methanol extracts. The total contents of phenolics and total flavonoids were identified by colorimetric method.The kinds and contents of bioactivities of flavonoids were identified by ultra-high performance liquid chromatography-quadruple time-of-flight-mass spectrometry( UHPLC- QTOF-MS).The results showed that the methanol extracts of soymilk owned antioxidative activity. The antioxidative values showed no difference between DPPH and FRAP methods. Total phenolics content (285.77±2.93 μg/mL) was higher than total flavonoids content (11.27±0.51 μg/mL) in soymilk methanol extract. The compounds were identified and quantified by UHPLC- QTOF-MS.Among five bioactivities identified, 6,7,4'-Trihydroxyflavone and Rha-Ara-GlcA-SoyB showed positive correlation with their antioxidant values. However, genistein and daidzein perform negative correlation with antioxidant values. 6,7,4'-Trihydroxyflavone may be the main compound attributing to antioxidation of the commercial soymilk .
    49  Development of a Colloidal Gold Labeled Strip for the Rapid Detection of Clenbuterol
    XIAO Zhi-li YU Jian-hua LEI Hong-tao YANG Jin-yi SUN Yuan-ming
    2013, 29(8):2004-2010.
    [Abstract](1647) [HTML](0) [PDF 582.83 K](757)
    Abstract:
    To develop colloidal gold immunochromatographic technique for the rapid detection of Clenbuterol (CL), colloidal gold was prepared by reduction of HAuCl4 using trisodium citrate. Anti-CL monoclonal antibodies (MAb) were then labeled with colloidal gold to obtain colloidal gold labeled MAb. The CL-human serum albumin (HSA) and goat anti-mouse IgG were immobilized on the lateral flow membrane to construct the strip. To obtain the best performance of the strip, several conditions were optimized as follows: the diameter of the colloidal gold particle 15 nm, the amount of MAb for labeling 20 ?g/mL, the pH value 7.4, the conjugate treating buffer 0.05 mol/L phosphate buffer (pH 7.4) containing 0.1% bovine serum albumin (BSA) and the thickness of colloidal gold-labeled CL antibody in use 50 ?L/cm2. In addition, the antigen and goat anti-mouse IgG were coated at 0.5 mg/mL and 2 mg/mL respectively. Under the optimum conditions, the limit of detection (LOD) of the strip was 3 ng/mL for swine urine. Cross-reaction tests showed that negligible cross-reactivity of the strip with six ?-Agonist compounds including ractopamine, salbutamol, etc. was detected. Forty-two samples of swine urine were detected by the developed strip and the results were confirmed by enzyme-linked immunosorbent assay (ELISA) kit. The accordance rate of the two methods was 100 %. The determination of CL by the strip did not need any instruments, and the total time for the determination of one sample was only 5 minutes. This method was applicable for the rapid determination of CL in the field.
    50  Development of an Immunoassay for Detection of Naproxen in Health Liquor
    GUO Jie-biao HAO Qing-chen LI Xing-ping XIAO Zi-jun HE Ying-juan LIU Yan-shan GUO Jie-biao HAO Qing-chen LI Xing-ping XIAO Zi-jun HE Ying-juan LIU Yan-shan GUO Jie-biao HAO Qing-chen LI Xing-ping XIAO Zi-jun HE Ying-juan LIU Yan-shan GUO Jie-biao HAO Qing-chen LI Xing-ping XIAO Zi-jun HE Ying-juan LIU Yan-shan GUO Jie-biao HAO Qing-chen LI Xing-ping XIAO Zi-jun HE Ying-juan LIU Yan-shan GUO Jie-biao HAO Qing-chen LI Xing-ping XIAO Zi-jun HE Ying-juan LIU Yan-shan
    2013, 29(8):2011-2014.
    [Abstract](1474) [HTML](0) [PDF 533.32 K](857)
    Abstract:
    Naproxen,a nonsteroidal anti-inflammatory drug, was frequently reported being adulterated onto antirheumatic health liquors to promote therapeutic effect. This phenomenon poses a serious threat to public health. In order to develop an immunoassay for detection of naproxen, providing an analytical method for rapid screening of illegal products, naproxen was conjugated to BSA and OVA to synthesize artificial antigens using active ester method for preparation of immunogen and testing antigen, respectively. Immunogen was employed to immunize rabbits for raising polyclonal antibodies. An enzyme-linked immunosorbent assay (ELISA) was established based on the antibodies and testing antigen. The IC50 value and LOD of the optimal ELISA were 23.0 ng/mL and 3.1 ng/mL, respectively. The recoveries were 87.3 ~102.1% and the coefficients of variation (CV%) were less than 8.9% when detecting Naproxen in health liquors. All cross-reactivities against 8 associate drugs were less than 0.05%. The ELISA method was in good agreement with LC-UV when detecting Naproxen in 1 positive and 19 negative health liquors from market. The method was suitable for screening Naproxen as illegal additives in health liquors due to its sensitivity and specificity.
    51  Nutrition Assessment and Anti-Fatigue Effect of Black Soybean Nutrient
    DENG Yuan-yuan WEI Zhen-cheng ZHANG Ming-wei ZHANG Rui-fen ZHANG Yan TANG Xiao-jun MA Yong-xuan
    2013, 29(8):2015-2019.
    [Abstract](1068) [HTML](0) [PDF 548.89 K](966)
    Abstract:
    A kind of nutrient was made from black soybean and black sesame through extruding, blending and grinding. Its nutrient components were determined with the national standards. Kunming mice were used to evaluate the anti-fatigue activities of the black soybean nutrient (black soybean nutrient was added in the feed) with different dosages(15, 30, 45 g/kg?bw) for 45 days, by measuring the swimming time, serum urea, hepatic glycogen and blood lactic acid. The results showed that the nutrient was in accordance with FAO/WHO high quality protein pattern. Amino acid score(AAS),chemical score(CS) and essential amino acid index(EAAI) of the black soybean nutrient was 1.02,0.65 and 78.77, respectively. Black health nutrition paste increased the swimming time of the mice and promote the level of their hepatic glycogen. In addition, the nutrient decreased the serum urea content and the area under the curve of blood lactic acid. The optimal dosage of nutrition paste was 30g/kg?bw.This research showed that the black soybean nutrient was a good protein supplement with alleviating fatigue function.
    52  Quality Evaluation of Frozen Vegetable Soybean Based on Principal Component Analysis
    JIANG Xiao-qing SONG Jiang-feng LI Da-jing LIU Chun-quan
    2013, 29(8):2020-2024.
    [Abstract](1311) [HTML](0) [PDF 487.09 K](721)
    Abstract:
    In order to study the quality of varieties of frozen vegetable soybean grown at the same production sites, 10 kinds of physiochemical indexes related to the quality of 13 kinds of frozen vegetable soybean were measured, including hardness, colour, chlorophyll, vitamin C, soluble protein, soluble sugar and so on. The main indexes were evaluated by principle component analysis (PCA). The results showed that four principal components could reflect most details on frozen vegetable soybean with a cumulative contribution rate of 89.33%. The first and second principal component showed simila the contribution rate for the frozen vegetable soybean, being of 28.13% and 26.55%, respectively. Soluble protein, colour, chlorophyll and hardness were main factors affecting the quality of frozen vegetable soybean. Xindali No.1, Tongdou No.6, Xudou No.17 and Tongdou No.5 were selected as the appropriate cultivars for vegetable soybean freeze-processing. The PCA comprehensive evaluation model showed a high positive correlation with the results of sensory evaluation, which could provide a certain theoretical basis to formulate the breeds and quality index of frozen vegetable soybean.
    53  Detection of 6 Preservative Residues of in Orange by High Performance Liquid Chromatography
    FENG Xia-ping ZENG Xian-yuan FU Li-min TANG Li-na HUANG Xiu-li CHEN Qing-qing
    2013, 29(8):2025-2029.
    [Abstract](1043) [HTML](0) [PDF 532.68 K](908)
    Abstract:
    A method was established to detect 6 kinds of fruit preservatives including p-methyl hydroxybenzoate, p-ethyl hydroxybenzoate, ethyl naphthol ,4-phenyl phenol,2,4-dichlorobenzene oxygen ethanoic acid (2,4-D) and diphenyl ether in orange by High Performance Liquid Chromatography (HPLC). The samples were extracted by ether and purified by activated carbon column and analyzed on a reversed phase C18 column using 55% CH3OH-45% H2O (pH 3) as mobile phase with a Diode array detector at 208 nm. The calibration curves showed good linear relatioship between the peak areas and concentrations of 6 preservatives in the range of 1.2~4.0 μg/mL, with correlation coefficients more than 0.999. Standard addition recovery ranged from 85.65% to 106.50% with relative standard deviation (RSD) from 2.36% to 6.78%. The limit of detection of ethyl naphthol was 0.5 μg/kg and the other five were 1.0 μg/kg. The method was simple, rapid and accurate, with high sensitivity and reproducibility, which was suitable for the analysis of the 6 preservatives mentioned above in orange.
    54  Separation and Identification of Main Pathogen in Hami Melon during Cold Storage
    LIU Tao TONG jun-mao MA Wei-rong WANG Jian SHAN Chun-hui
    2013, 29(8):2030-2034.
    [Abstract](1287) [HTML](0) [PDF 538.00 K](732)
    Abstract:
    Pathogenic bacteria which may cause the rot of melon was separated and identified from rotting postharvest Xinjiang Hami melon during cold storage, by using traditional pure culture method, dilution spread plate method and fungal genomic DNA isolation Kit method. The physiological and biochemical properties of the strains, as well as their 26s rRNA gene sequence, were analyzed. Taking NL1 and NL4 as the universal primers, 5 strains of pathogenic bacteria were separated and confirmed as Fusarium, Stachybotrys, Penicillium and Gibberella. The BJ-M and BJ-M1 strains belonged to the genus Fusarium and BJ-M2 strains belonged to Gibberella. BJ-M3 strains belonged to Stachybotry and BJ-Q strains belong to the genus Penicillium. This research may provide theoretical basis for storage period of Hami Melon by investigation of the pathogenic bacteria.
    55  Advance in Bioactivity and Safety of Peptide Chelated Zinc
    ZENG Qing-zhu CHEN Lu-xin
    2013, 29(8):2035-2039.
    [Abstract](1461) [HTML](0) [PDF 468.57 K](940)
    Abstract:
    Peptide chelated zinc is a new organic metal compound with various bioactivities. It is mainly prepared by three methods, including synthesis with zn2+ and artificial peptide, synthesis of zn2+ and natural peptide by enzymolysis of plant and animal protein, and extraction from plant and animal tissue. Due to the difference of peptide ligands, the peptide chelated zinc showed varied structure and bioactivities including antibacterial and bacteriostasis effect, regulations of physiological functions and immune, and antioxidation.. The recent researches of bioactivities and safety of peptide chelated zinc were reviewed in this research. Suggestion were proposed on more investigation of elative toxicology and applying safety of this compound, to promote the potential industrial application of peptide chelated zinc

    Editor in chief:李琳

    Inauguration:现代食品科技

    International standard number:ISSN 1673-9078

    Unified domestic issue:CN 44-1620/TS

    Domestic postal code:46-349

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