Volume 29,Issue 6,2013 Table of Contents

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  • 1  Evaluation of the in vitro Antioxidant Activity and in vivo Blood Lipid-lowering Capability of Chenxiang Tea Extracts
    CHEN Di-ling WU Yi LIN Li SHUAI Ou WANG Ke-yuan ZHANG He-ming LIU Song-ha
    2013, 29(6):1198-1201.
    [Abstract](1600) [HTML](0) [PDF 381.07 K](708)
    Abstract:
    This experiment studied the chengxiang tea extracts from the raw materials of leaves of Aquilaria sinensis (Lour.) Gilg. Its radical scavenging activity, reducing power and metal chelating activity were determined to assays the in vitro antioxidant activity, and hyperlipidemia model mice were used to evaluate the blood lipid-reducing capacity of the water-extracts from Chenxiang tea. The results showed that the water extracts from Chenxiang tea have strong free radical scavenging activity, effective reducing power and chelating ability, with their IC50 values being of 700.45±38.04, 58.03±4.4, 16.24±2.4, 70.69±2.8 and 215.46±14.27 mg/mL, respectively. The tea extracts can reduce the levels of TG and TC and increase the level of HDL-C in hyperlipidemia model mice serum. Compared with the model group, the differences were significant (P<0.05).
    2  Effect of Acid & Heat-moisture Treatment on Properties and EnzymaticKinetics of Wheat Flour
    HUANG Li-xin ZHANG Xin-xin WU Xiao-yuan GUO Feng
    2013, 29(6):1202-1206.
    [Abstract](1211) [HTML](0) [PDF 498.47 K](752)
    Abstract:
    Wheat flour was modified by acid and heat-moisture treatment in thermal water kettle.Polarization microscope, scanning electron micrograph, differential scanning calorimetry (DSC) instrument, Brabender viscosity instrument, X-ray diffraction (XRD), infrared spectrometer were used to study the properties enzymatic kinetics of wheat flour before and after the treatment. It was found that, after modification, the protein content was reduced and part of starch granules had shallow crack. Polarization cross was blurring. DSC showed endothermic peak moved backward and absorption enthalpy (ΔH) decreased 2.976 J/g. Brabender viscosity curve indicated that pasting temperature increased from 82.6 ℃ to 87 ℃ and viscosity decreased from 82 BU to 63 BU. XRD showed crystalline structure of starch increased 3.8%. Under in vitro digestion conditions, α-Amylase hydrolyzation of treatment product followed Michaelis-Menten, and enzymatic kinetic equation of product with α-Amylase was obtained as V=0.161[S]/(1.093+[S]). Compared with that without modification, Michealis constant (Km) and maximum rate (Vm) decreased.
    3  Characteristics of Ganoderma lucidum Polysaccharide Prepared by Different Methods
    YOU Li-jun FENG Meng-ying LIU Jun-fa ZHAO Qiang-zhong LUO Wei ZHAO Mou-ming
    2013, 29(6):1207-1212.
    [Abstract](1468) [HTML](0) [PDF 574.70 K](975)
    Abstract:
    Different extraction methods, including ultrasound extraction (UP), ultrasonic wave complex cellulose (UCP), ultrasonic wave complex pancreatin (UPP) and traditional hot water extraction (TP), were used to prepare Ganoderma Lucidum polysaccharide and the characteristics of the extracted polysaccharides were compared. Results showed that the extraction yield of polysaccharides by UPP reached 4.93%, 1.58 times higher than that by TP. The molecular weight of polysaccharides by UP was smaller than that by TP. The lowest molecular weight (<5 kDa) was found when UPP was employed. Moreover, the polysaccharides by UPP exhibited the highest reducing power (1.408 at 1.0 mg/mL), hydroxyl radical scavenging ability (50.08% at 2.0 mg/mL), ABTS+scavenging ability (82.5% at 1.0 mg/mL) and the oxygen radical absorbance capacity (2484.14 μmol Trolox/g).
    4  Structural Identification and Antioxidant Activity of the Flavones from Laggera pterodonta
    LI Shu-hua ZHAO Qi LIU Fang LEI Kai-ju
    2013, 29(6):1213-1216.
    [Abstract](841) [HTML](0) [PDF 465.06 K](756)
    Abstract:
    The plant of Laggera pterodonta was powered, extracted with ethanol and purified by silica gel column chromatography. The purity of the compounds from the plant was investigated by HPLC and their structures were identified on the basis of spectral analysis. Then the antioxidant activity of the compounds was evaluated by DPPH radical scavenging assay. Artemitin and chrysosptertin B were separated from Laggera pterodonta, which showed antioxidant activity lower than vitamin C.
    5  Analysis of Microbial Community Structures in Kefir Grains By PCR-DGGE Techniques
    LIN Xiao-shan RUAN Zheng LIU Fei ZHANG Yi
    2013, 29(6):1217-1221.
    [Abstract](1378) [HTML](0) [PDF 695.63 K](706)
    Abstract:
    The dynamic variation of microbial flora in kefir liquor during its fermentation was studied by PCR-DGGE technique. The analysis result showed that 3 different visible bands in bacteria community and only 1 band in yeast community were obtained in their electrophoresis pattern. Anaylsis of 16SrDNA sequence blast showed that these sequences of all 3 different visible bands had a high homology with the sequence of 16S rDNA of lactic acid bacteria (LAB). They belonged to Leuconostoc mesenteroides, Lactobacillus kefiranofacien and Lactobacillus kefir. The sequence of the band in yeast community had a high homology with the 16S rDNA of yeast Torulaspora delbrueckii. Yeast Torulaspora delbrueckii was, for the first time, found in kefir grains.
    6  A New Method for Enzymatic Synthesis of Xylose Diester in Two-solvent Medium and its Structural Identification
    FENG Guang-li LI Xiao-feng ZHANG Ben ZHAO Guang-lei
    2013, 29(6):1222-1226.
    [Abstract](1358) [HTML](0) [PDF 665.52 K](807)
    Abstract:
    A new method for synthesis of xylose esters based on solvent strategy was developed in this paper. Binary organic solvent of n-hexane and pyridine (25:75, V/V) was used as reaction system to well dissolve sugar substrate and meanwhile maintain the enzyme activity. Immobilized lipase CAL type B, Novozyme 435, was chosen as a biocatalyst to synthesize xylose ester via acylation of xylose and vinyl methacrylate. HPLC analysis showed that the highest substrate conversion (>90%) was obtained when the ratio of xylose to vinyl methacrylate, shaking speed and reaction temperature were 1:10, 150 r/min and 30 ℃, respectively. The products were structurally identified by GC-MS and 13C-NMR as a diester of xylose, 1′, 5′- dimethacrylic xylose ester. This research provided an efficient and environmentally-friendly method with application potential in production of amphoteric compounds used in food, chemical and papermaking industrils.
    7  Dephlogisticate, Antitussive and Expectorant Effects of Polysaccharides from Exocarpium citri grandis on Mice
    HOU Xiu-juan SHEN Yong-gen XU Ming-sheng FAN Shao-wu HUANG Liang
    2013, 29(6):1227-1229.
    [Abstract](1247) [HTML](0) [PDF 448.57 K](797)
    Abstract:
    The dephlogisticate ,antitussive and expectorant effects of the polysaccharides from Exocarpium citri grandis (ECP) were studied. The models of edema induced by xylene, cough induced by concentrated ammonia water and phenel red execretion in mice were used to observe the dephlogisticate effect, the antitussive effect and the expectorant effect, respectively. Result showed that ECP could inhibit the edema induced by xylene in mice. The swelling inhibition rates in low, middle and high dose group were 24.93%, 34.84% and 48.72%, respectively. The swelling inhibition rate in the positive control group was 52.83%. It was also found that ECP could prolong cough incubation period and reduce the cough times in 2 minutes induced by concentrated ammonia water. There was a significant difference (P < 0.01) between the control group and the middle/high dose groups, which meant that the ECP could increase phenel red execretion in mice. ECP showed a significant dephlogisticate, antitussive and expectorant effects.
    8  Effect of Sodium Citrate and Biotin on ε-Poly-lysine Fermentation of Streptomyces albulus
    WANG Feng SHI Kan PAN Tao WU Qing-ping MO Shu-ping WU Zhen-qiang
    2013, 29(6):1230-1233.
    [Abstract](950) [HTML](0) [PDF 560.84 K](783)
    Abstract:
    The effect of sodium citrate on ε-PL fermentation of S. albulus was investigated. The results showed that sodium citrate concentrations had slight effect on cell growth, but significantly affect the synthesis of ε-PL. The addition of 2 g/L sodium citrate at 0 h resulted in the highest ε-PL concentration of 0.92 g/L. By adding 2 g/L sodium citrate at 0 h and 300 μg/L biotin at 36 h to fermentation media, the cell dry weight and ε-PL yield reached the highest values of 7.86 g/L and 1.10 g/L, respectively, being 1.30 and 1.93 folds to the control respectively. It was demonstrated that sodium citrate and biotin will promote cell growth and ε-PL yield of fermentation of S. albulus.
    9  Quality Change of Wonhuwang Pear Cloudy Juice during Storage
    FAN Ming-yue SONG Li-ning WANG Cheng-rong WANG Ran
    2013, 29(6):1234-1236.
    [Abstract](1064) [HTML](0) [PDF 432.73 K](787)
    Abstract:
    Maillard reaction of the cloudy juice that prepared from Wonhuwang pear was studied during the storage at different temperature. The results showed that pH, reduced sugar, amino acid and soluble protein had a decreasing trend. Total color aberration and 5-HMF rosed gradually during the storage at different temperatures. Cloudy Wonhuwang pear juice suffered lower change of physicochemical indexes during storage at 0 ℃ than at 4 ℃, which indicated that 0 ℃ was the better storage temperature for cloudy Wonhuwang pear juice due to the slowed speed of Maillard reaction.
    10  Structure and Emulsifying Properties of Sugar Beet PectinFractionated by Ultrafiltration
    WU Guo-li FU Xiong MENG He-cheng ZHAO Zheng-gang YANG Xiong
    2013, 29(6):1237-1242.
    [Abstract](1213) [HTML](0) [PDF 505.74 K](882)
    Abstract:
    Pectin extracted from sugar beet was fractionated into four fractions by filtration. The structure and emulsifying properties of pectin from the fractios were characterized by HPLC, FT-IR and GC. The results showed that the content of pectin was the highest (64.86%) when the MWCO size membrane was above 100000. Molecular weight of beet pectin was related to its physicochemical properties. And the galacturonic acid content, the ferulic acid content and degree of esterification increased with the increase of the molecular weight. The pectin emulsifying properties depended on the molecular weight. The pectin with the molecular weight of 4.64×104 Da showed the highest emulsifying activity and stability.
    11  Effects of Compound Preservatives on Senescence of Postharvest Broccoli under Micro-vacuum Conditions
    WANG Li-jiao SUN Xing-li YUE Ben-fang LI Wen-xiang WANG Shi-kui ZHANG Sheng-jie
    2013, 29(6):1243-1246.
    [Abstract](1165) [HTML](0) [PDF 641.73 K](770)
    Abstract:
    In order to explore the effects of compound preservatives of 6-BA and 0.2% SBN on senescence of postharvest broccoli in micro-vacuum conditions, freshly broccoli was stored at temperature of 3±0.5 ℃ with pressure of 400~500 torr. The contents of chlorophyll, Vc and MDA and the activities of PPO and POD were determined to explore better composite preservative concentration. The results showed that three concentrations can inhibit the degradation of chlorophyll and Vc, reduce the accumulation of membrane lipid peroxidation product MDA, lower the activities of PPO and POD in varying degrees. 40 mg/L 6-BA and 0.2% SBN showed the best effect on inhibition of broccoli postharvest senescence.
    12  Antimicrobial and Adhesion Properties of Four Lactobacillus Strains
    YU Han-li TUO Yan-feng AI Lian-zhong WU Zheng-jun GUO Beng-hen CHEN Wei
    2013, 29(6):1247-1251.
    [Abstract](890) [HTML](0) [PDF 442.96 K](690)
    Abstract:
    The present work explored the antimicarobial and adhesion property of four lactobacillus strains with probiotic potential and compared with the typical probiotic L. rhamnosus GG, by investigation of their acid and bile torelance, antibiotic susceptiblity, antagonism against important gastrointestinal pathogens and their ability of adhere to Caco-2 cell. Lactobacillus casei 142 and L. plantarum 89 can survived in both the acidic and bile salt environments. All lactobacillu strains tested were sensitive to the antibiotics used in this investigation and had strong antimicrobial effect against the pathogens. L.plantarum 89, L.plantarum 27 and L.plantarum 13 showed strong adhension abilities, with their adhension capacity to the Caco-2 cell being of >20 cfu/cell. However, L. casei 142 showed no adhesion ablity to Caco-2 cells. The results suggested the probiotic potential of the four strains.
    13  Effect of Drying Temperature on Quality of Pleurotus eryngii
    YOU Chu-zhen LIN Jun-fang GUO Li-qiong SUN Ping BAI Wei-fang
    2013, 29(6):1252-1254.
    [Abstract](1139) [HTML](0) [PDF 330.64 K](673)
    Abstract:
    In this research different parts of Pleurotus eryngii were chosen as the materials, dried and rehydrated under different temperatures. The nutrient content and sensory properties of the samples with the best and worst rehydration ratio were detected. The results showed that the nutrient content of Pleurotus eryngii dried at 70 ℃ was higher than that at 40 ℃. The hardness and chewing of the top and middle parts of Pleurotus eryngii dried at 70 ℃ were higher than that at 40 ℃, while contrary results were found for the bottom parts tested. The elasticity of top parts of Pleurotus eryngii dried at 70 ℃ had a large difference with that at 40 ℃, and showed little difference with the other parts.
    14  Isolation and Characterization of Corn Germ Protein
    SONG Chun-li REN Jian MA Yun-fei
    2013, 29(6):1255-1258.
    [Abstract](902) [HTML](0) [PDF 442.32 K](796)
    Abstract:
    In this work, the isolation and characterization of corn germ protein extracting by traditional method was studied. The results showed an amino acid balance in the obtained protein except for Glu and Lys which showed high contents. SDS-PAGE analysis revealed that there were nine components in corn germ protein isolate with the molecular weight being of 87.988, 76.320, 69.650, 53.649, 48.546, 38.164, 34.337, 32.294 and 24.483 kDa, respectively. Two fractions separated from corn germ protein by gel filtration chromatography were obtained. Differential Scanning Calorimetry (DSC) analysis showed that the denaturation temperature of the obtained fractions were 97.37 and 98.95 ℃, respectively. The secondary structure analyed by (CD) revealed that the main conformation of the obtained fractions were β-sheet and random coil.
    15  Anti- Helicobacter pylori effect of 6-gingerol in vitro
    ZHANG Yun-ling ZHENG Yi-min HU Shao-nan LIU Hong
    2013, 29(6):1259-1261.
    [Abstract](1162) [HTML](0) [PDF 420.99 K](684)
    Abstract:
    Study on the in vitro anti-HP (helicobacter pylori) effect of 6-gingerol in the gingerols of ginger. Use ethylalcohol 95% and tap water to collect gingerols contained in ginger with amoxicillin as the positive control and 50% PEG400 as the blank control, and test the Minimal Inhibitory Concentration (MIC) concerning the in vitro anti-HP effect of 6-gingerol in the gingerols of ginger. In respect of the in vitro anti-HP effect, The MIC of the ethanol extract of ginger is 1.00 mg/mL, the 6-gingerol 0.02 mg/mL. The aqueous extract of ginger has no antibacterial effect in vitro. The bacteriostatic active material contained in the ginger is 6-gingerol in the gingerols.
    16  Changes in Yolk Protein Secondary Structure of the Preserved Egg in Wine during Low Pressure-vacuum Processing
    WANG Hong-ni LIU Hui-ping LIU Ping-wei YUAN Wei
    2013, 29(6):1262-1265.
    [Abstract](1049) [HTML](0) [PDF 438.80 K](878)
    Abstract:
    The research examined the secondary structure of yolk proteins of the egg preserved in wine and treated by low pressure vacuum for 0-45 days, using Fourier transformed infrared spectrum (FTIR). Noticeable changes in β-sheet ( up to 37.70%) and β-turns (reduce to 16.32%) were found when the egg pickled for 30 days. The structures of β-sheet and β-turns were transformational with each other during pickling. On the other hand, the content of α-helix and random coils had little changes. The results indicated the secondary bonds of yolk proteins of egg preserved in wine were broken effected by ionic strength (Ga2+ and Na+) under acid conditions. The results also showed the degeneration of yolk proteins were accelerated by low pH and ionic strength.
    17  Isolation and Characterization of Bacillus licheniformis Phage
    WANG Feng-chao LIU Wen-yao WANG Hai
    2013, 29(6):1266-1269.
    [Abstract](1080) [HTML](0) [PDF 466.51 K](798)
    Abstract:
    A virulent bacteriophage of Bacillus licheniformis was isolated from the nearby drainage and air environment of alkali protease producing factory. Electronic microscopy showed that the phage had an isometric head (59 nm in diameter) and a long non-contractile tail (132 nm in length), which indicated the characters of Siphoviridae family. Bacillus licheniformis was the host cell for the phage in the test. The study revealed that the phage had good stability at pH 5~0 and certain stability under high temperature. The invasion to the host cell could be accelerated by metal ion. One-step growth kinetics showed that the latent period of the invasion was about 40 min, and the rise period was about 110 min. Double-stranded linear DNA was the genetic material of the phage, with a molecular weight of about 36 kb.
    18  Inhibition Effect of a Compound Preservative onSpoilage Bacteria in Herbal Tea
    XU Qing XU Xi-lin LI Zhi-min
    2013, 29(6):1270-1272.
    [Abstract](963) [HTML](0) [PDF 330.62 K](665)
    Abstract:
    Separation and identification of spoilage bacteria from herbal tea showed that the main spoilage organisms in the herbal tea were Gram-positive Bacillus bacteria (Paenibacillus polymyxa,Bacillus pumilus and Bacillus megaterium). The better preservative for inhibition of the spoilage bacteria in herbal tea were found as Nisin, Nipagin ester and Chitosan. The formulation of a compound preservative with the three preservatives for enhanced efficacy was optimized by response surface methodology. A quadratic polynomial regression model was built using Box-Behnken design, The best composite bacteriostatic agent formula and the antibacterial effects of preservatives combination were validated.
    19  Influence of Ascorbic Acid and Chitosan on Selenium Enrichment of Soybean
    HUANG Yan-sheng NING Zheng-xiang WU Xiao-yong
    2013, 29(6):1273-1276.
    [Abstract](1083) [HTML](0) [PDF 461.57 K](737)
    Abstract:
    In order to improve the selenium enrichment effect of soybean, the influence of soaking condition, ascorbic acid and chitosan on the sprouting rate and sprout length of the selenium enrichment soybean were investigated in this study. Results indicated that the ascorbic acid will improve the germination and growth of soybean seeds, and will disadvantage to the selenium enrichment of soybean, but after adding chitosan to the soaking solution, the negative influence of the ascorbic acid will extinction. The best formula of soaking solution contained 60 μg/mL Na2SeO3, 0.08% ascorbic acid and 0.1% chitosan. The best soaking time and temperature were 8 h and 30 ℃, respectively. After processing, total selenium content and the isolated protein of the selenium-enriched soybean reached 27.31 μg/g and 31.95 μg/g, respectively.
    20  Changes of Nutrition Components of Bean Residue Fermented by Different Fungus
    WANG Hui LIU Ying HU Bo-han LIU Su-chun
    2013, 29(6):1277-1280.
    [Abstract](1142) [HTML](0) [PDF 446.82 K](835)
    Abstract:
    Bean dregs were fermented by using three kinds of fungus (Rhizopus, Mucor, and Aspergillus oryzae). The nutritional ingredients, protease and amylase activity of the bean dregs during 6 periods of fermentation were determined. The results showed that the quality of fermented bean dregs was improved obviously after the fermentation. The amino nitrogen content and total soluble sugar content of bean dregs increased significantly after fermentation, but the fat content decreased. Protease and amylase activities of bean dregs rose gradually in the initial stage of fermentation.The maximum of protease activity of fermented bean dregs by Mucor and Rhizopus reached 96.4 U/g and 13.2 U/g, respectively.
    21  Effects of Experimental Conditions on the Texture Properties of Set-style Yoghurt in TPA Test: an RSM Approach
    YANG Ying-ying HE Hong-jun GUO Meng-meng SUN Ning LI Xing
    2013, 29(6):1281-1284.
    [Abstract](1067) [HTML](0) [PDF 471.33 K](755)
    Abstract:
    In this work the influence of the degree of deformation and compression speeds on the mechanical parameters of set-style yoghurt in TPA test was studied.by response surface methodology. Results showed that the degree of deformation had very significant effect on hardness, adhesiveness, cohesiveness and gumminess of the yoghurt (P≤0.0001). Compression speed had significant influence on hardness and adhesiveness(P≤0.0001). Compression speed had significant influence on cohesiveness(0.01
    22  Preservation Effect of Amomum tsao-ko Extract on Carp
    LUO Shi-shu LIU Xiao-ling
    2013, 29(6):1285-1287.
    [Abstract](1174) [HTML](0) [PDF 451.25 K](812)
    Abstract:
    The preservative effect on the fish of Amomum tsao-ko extract was investigated by soaking the fish in 0.3% Amomum tsao-ko extract and compared with that of 0.3% potassium sorbate solution. The results showed that Amomum tsao-ko extract can significantly inhibit bacterial growth and reproduction in the fish, and slow down the increase of TVB-N and pH values of the fish. In addition, it can lower the decline rate of the sensory quality of the fish. The Amomum tsao-ko extract has similar antibacterial and antioxidant effects to potassium sorbate.
    23  Optimization of Processing Conditions for Chicken Liver Sausage by Response Surface Analysis
    LIU An-jun GUO Jing WANG Zi-jian ZHENG Jie TENG An-guo WANG Jiao-jiao
    2013, 29(6):1288-1292.
    [Abstract](891) [HTML](0) [PDF 540.76 K](756)
    Abstract:
    Chicken liver was used as main ingredients to prepare a new kinds of sausage. The physical properties of the products were analyzed, including the hardness, flexibility, adhesion and chewiness to investigate the influences of water, soy protein ioslate and chopping time on the quality of the liver sausage. Then Box-Behnken design was applied to optimize the processing conditions of liver sausage. The optimal formula was determined as chopping time 8.3 minute, soy protein isolate1.99%, water amount 39.98 mL. With the optimal formula, the liver sausage showed the best quality, with sensory scores and flexible being of 92.90 and 0.85, respectively.
    24  Screening and Fermentaion of Yeast for Fig Fruit Wine Production
    ZUO Yong LIU Li-ping JU Shuai XIE Hui XIE Hui QI Feng
    2013, 29(6):1293-1296.
    [Abstract](1159) [HTML](0) [PDF 534.30 K](710)
    Abstract:
    From 41 yeast strains preliminarily isolated from fig skin and fig wine lees,12 strains was screened due to their capability of producing better wine fragrants. Then 6 strains showing fast fermentation speed were selected by duchenne tube method. Comparison of the fig sauce fermentation by the strains showed that yeast Z20 gave fast fermentation speed and produced better wine fragran. The tolerability of the strain was measured and the result showed that the Z20 yeast can tolerate high sugar content, acidity, SO2 and ethanol, which could be used for fig fruit wine fermentation.
    25  Application of Artificial Neural Network to Relative Humidity Prediction
    GUO Qing-chun HE Zhen-fang XI Ying LI Xue
    2013, 29(6):1297-1301.
    [Abstract](1224) [HTML](0) [PDF 491.39 K](700)
    Abstract:
    Relative humidity environment is an important item of agricultural production monitoring and forecasting. Relative humidity concerns to plant growth condition, ecological prevention and control of several plant diseases, irrigation regulation. Because relative humidity has many affecting factors, there are nonlinear relations between relative humidity and its influence factors. An improved BP artificial neural network was put forward as the relative humidity prediction model, in order to improve the relative humidity prediction accuracy. The model can predict the air relative humidity with such inputs as the weather data (hours of sunlight, precipitation, the lowest temperature, the average temperature, the highest temperature). Experiments were carried out in Lingshui area to collect data to validate the model. The simulation results indicate that the monthly average relative humidity for the period of 1987~1998 was simulated and predicted for the period of 1999~2000. Relative error between the simulation and measured air relative humidity was 0.21%, and relative error between the prediction and measured air relative humidity was 0.28%. The improved BP artificial neural network has higher prediction precision. It was simple that using the BP model method to study relative humidity. And also it can provide reference for the other area’ research about relative humidity.
    26  Effect of AWRi R2 Saccharomy Cescerevisiae on the Fermentation Process of Banana Brandy
    YUAN De-bao ZHENG Jie-qiong LI Fen-fang TAN Lin LI Yi-xing CHEN Jiao ZHENG Xiao-yan ZHENG Li-li JIN Zhi-qiang
    2013, 29(6):1302-1305.
    [Abstract](1136) [HTML](0) [PDF 471.42 K](822)
    Abstract:
    In order to explore the role of AWRi R2 saccharomy cescerevisiae in the fermentation process of banana brandy, the effect of technological parameters such as fermentation temperature, inoculum size and initial appearance Brix on indicators related to banana brandy fermentation process, such as pH, total acid, total sugar, reducing sugar, and alcoholicity, were studied. First, the results of the growth curve of AWRi R2 saccharomy cescerevisiae at different temperatures showed that, the biomass of the bacteria increased as the culture temperature rose, and the logarithmic growth phase was about 4~20 h in the whole incubation time. The fermentation temperature had great effect on the fermentation process, i.e., the stability of the pH value (or total acid content), the consumption of sugar and the amount of ethanol production were better than other temperature groups, when the fermentation temperature was 28 ℃. When the inoculum size was 2%, the alcoholicity was highest. Taking the sugar consumption rate and the production of alcohol into consideration, the initial appearance of Brix was 20% was best. This study laid a certain foundation for the application of AWRi R2 Saccharomy cescerevisiae in banana brandy fermentation.
    27  Preparation of Sea Buckthorn Strengthen Vc Lozenges
    ZHOU Wei-xi TANG Ping HU Huai-rong SHI Guo-hua LI Ming-yuan
    2013, 29(6):1306-1310.
    [Abstract](995) [HTML](0) [PDF 492.20 K](793)
    Abstract:
    The Vc lozenge of seabuck-thorn use Sea-buckthorn juice concentrate as raw material and through comparative trial to choose the suitable accessories, recipe and production process conditions. Then analyse the quality of the product. The results show that the best combination: 6.5% of Seabuck-thorn juice concentrate, 38.9% of the white sugar, 26.4% of starch, 26.4% of lactose, 1.6% of citric acid, 0.05% of menthol, 5‰ of magnesium stearate; The optimum process conditions: 16-mesh sieve granulation was dried for 20~30 min under 55~60 ℃, 16 or 18 mesh sieve granulated. The Vc lozenge was produced in this formula and process conditions can meet the requirements such products relevant national standards.
    28  Optimization of Hurdle Pattern for Preservation of Ready-to-eat Abalone (Haliotis discus hannai Ino) Based on Neural Network
    XI Qing ZHANG Chao-hua ZENG Shao-kui
    2013, 29(6):1311-1315.
    [Abstract](1152) [HTML](0) [PDF 523.29 K](709)
    Abstract:
    Based on the theory of hurdle effect, the effects of different hurdle pattern formed by different hurdle factor and factor intensity on the antibacterial action and quality of ready-to-eat abalone were studied. A neural network model was established and the hurdle pattern was optimized. The result showed that the best hurdle pattern was: aw 0.92, Nisin 0.39 g/kg, Sodium lactate1.6% and temperature 89 ℃, under which the longest storage time of ready-to-eat abalone was achieved and the products can maintain their special taste and texture during strorage.
    29  Optimization of Ultra High Pressure -assisted Extraction of Tea-polyphenols by Response Surface Methedology
    JIANG Dong-wen HUANG Jia-jia YANG Gong-ming DU Bing
    2013, 29(6):1316-1320.
    [Abstract](833) [HTML](0) [PDF 470.11 K](820)
    Abstract:
    To obtain the optimum ultra high pressure-assisted extraction technology of tea-polyphenols from low value tea. On the basis of single-factor test, the mathematical regression model is established about the dependent variable(extraction yield of tea-polyphenols ) and independent variables (alcohol concentration, time of maintain pressure and extraction pressure) through Box-Benhnken center composite design and response surface methodology. The results indicated that the optimal extraction conditions were alcohol concentration of 60%, processing time of 1.5 min, extraction pressure of 300 MPa. Under these conditions, the predictive maximum yield of tea-polyphenols from low value tea was 31.07%, the validation value was 30.87%. The relative error between them was 2.59% .The standardized regression equation was Y1=776.3561-1.3356X1+123.093X2+163.7406X3-274.2023X1X1-145.2251X2X2.
    30  Preservation of Amydalus persica cv. Okubao by Refrigeration
    WANG Jing MIAO Li-jun
    2013, 29(6):1321-1323.
    [Abstract](1159) [HTML](0) [PDF 425.89 K](691)
    Abstract:
    The effects of the refrigerating temperature, package mathod and time on preservation of fresh and canned peach of Amydalus persica cv. Okubao were studied. Results showed that, the most suitable refrigerant temperature for the peach was (1±1) ℃, under which the fresh and canned peaches packaged in 0.3 mm PE bags showed the best sensory quality and physiochemical indexes in a 30-day storage. The utilization rate of materials for canned peach production reached >76.32%, 26.32 percent higher than that by traditional preservation method. The study provided references for the preservation of Amydalus persica cv. Okubao for the production of canned soft white peach.
    31  Extraction of Resveratrol from Polygonum cuspidatum
    YE Qiu-xiong HUANG Wei
    2013, 29(6):1324-1327.
    [Abstract](1134) [HTML](0) [PDF 442.27 K](780)
    Abstract:
    Resveratrol is an important ntural compound with bioactivity. In this experiment, Polygonum cuspidatum was used as raw materials for extraction process f resveratrol. Effects of solid-liquid ratio, raw material particle size, ethanol concentration, extraction time, extraction temperature were investigated on the extraction rate. The orthogonal test was used to determine the optimum conditions with solvent and enzymatic extraction of resveratrol in Polygonum cuspidatum. The results showed that the best solvent extraction conditions were pH 5, solid-liquid ratio 1:65 (g:mL), temperature 60 ℃ and extraction time 120 min; The optimum conditions for enzymatic extraction were pH 6.0, solid-liquid ratio 1:40 (g:mL), hydrolysis temperature 60 ℃, and hydrolysis time 120 min. The results showed that, the yields of solvent and enzymatic extractionwere 0.496% and 0.942%, respectively. Compared with solvent method, extraction yield of resveratrol by using enzymatic method was increased by about 2 times. Enzymatic method can be used as the preferred method of extraction of resveratrol.
    32  Preparation of a New Cool Beverage Containing Poria and Coix
    LI Shu-yi GU Cai-qin ZHOU Xue-e LAI Chun-hua MAI Zhi-an ZHENG Xu-dong
    2013, 29(6):1328-1332.
    [Abstract](932) [HTML](0) [PDF 497.93 K](789)
    Abstract:
    The optimal formula and procession parameter of a new cool beverage containing Poria, Coix and other additives (the root of kudzu vine, liquorice, red dates, xanthan gum and sirup) were studied by one factor and orthogonal test. The results showed that the optimal formula of cool beverage contained 6% of the mixture of poria and the root of kudzu vine (1:0.6), 0.75% of Coix, 0.65% of liquorice, 1.25% red dates, 0.18% xanthan gum and 5.50% sirup. The best cooking time was determined as 30 min. Analysis of 100 mL product showed that the product contained 59.80 mg tuckahoe polysaccharide and, 45.00 mg flavone of the root of kudzu vine. The soluble solid was 4.5. The beverage tasted sweet and delicious.
    33  Extraction of Pleurotus eryngii Polysaccharides with PEG/(NH4)2SO4 Two-Phase Aqueous System
    SU Yan-ling
    2013, 29(6):1333-1336.
    [Abstract](909) [HTML](0) [PDF 450.16 K](733)
    Abstract:
    The extraction of Pleurotus eryngii polysaccharides in two-phase aqueous system was investigated. The effects of PEG molecular weight, and concentrations of (NH4)2SO4 and PEG on the yield of Pleurotus eryngii polysaccharides were studied The results showed that the optimum conditions were as follows: concentration of PEG4000 21.33% and concentration of (NH4)2SO4 14%, under which partition coefficient of polysaccharide could reach 1.9529~2.0152 and the yield coefficient could reach 60.58~62.82 %.
    34  Synthesis of L-ascorbic Acid Cinnamic Acid Ester and Its Antioxidant Activities
    WANG Yuan HUI Ai-ling PAN Jian WANG Hao-shu SHI Lin-juan
    2013, 29(6):1337-1341.
    [Abstract](1254) [HTML](0) [PDF 622.01 K](735)
    Abstract:
    This experiment was obtained L-ascorbic cinnamic acid ester by esterification with L-ascorbic acid and cinnamic acid as raw materials. Stability of esterification product in solution clear hydroxyl free radical (?OH) and diphenyl generation bitter hydrazine group free radicals (DPPH?) ability were studied using spectrophotometric method. And the corresponding free radical activity was compared with Vc and tertiary butyl hydroquinone (TBHQ). The results showed thatL-ascorbic acid cinnamic acid ester had higher stability than Vc. The DPPH? scavenging ability of the product was similar to that of Vc, with the EC50 values being of 63.96 μmol/L. Its EC50 values for removing ?OH was of 7.84 mmol/L, better than that of TBHQ in high concentration.
    35  Effect of Carrageenan on the Quality of a Fruit-flavor Jam Used for Mooncake Preparation
    PAN Li-ying SONG Zhen-shan GUO Hua ZHOU Xue-song
    2013, 29(6):1342-1345.
    [Abstract](899) [HTML](0) [PDF 472.96 K](711)
    Abstract:
    Effects of carrageenan on the moulding, heat-resisting, oil-holding, water-holding, sensory properties and texture of the mooncake fruit-flavor jam were studied in this paper. The results showed that the optimal carrageenan dosage added to the mooncake fruit -flavor jam and ratio of fruit-flavor jam to red bean paste were 1.5‰ and 3:7, respectively, under which a new mooncake mixed fillings was achieved with good moulding, heat-resisting, oil-holding and anti-aging properties, without cracking and oil leaking in a 3-month storage. At the same time, the mixed filling not only retained the delicate structure of original red bean paste with moderate elasticity, but also reduced the sweetness of the traditional bean paste giving a better taste, which can be prepared and used in Cantonese mooncakes.
    36  Supercritical Fluid Extraction of Ergosterol from Fermented Ganoderma lucidum
    LIU Guo-jie
    2013, 29(6):1346-1348.
    [Abstract](1280) [HTML](0) [PDF 408.34 K](635)
    Abstract:
    Supercritical fluid extraction conditions of ergosterol from Ganoderma lucidum after fermentation were optimization by orthogonal test on the basis of single factor experiment. The results showed that the extraction pressure and temperature were significant factors and the optimization conditions were as follows: extraction pressure20 MPa, extraction temperature 50 ℃, and CO2 flow rate 2.5 L/min, under which the content of ergosterol reached 1.1362 mg/g.
    37  Optimization of Extraction of Proanthocyanidins from Skin of Red Locus Seed Using Response Surface Methodology
    PENG Fang-gang LI Qi-li WU Wei-guo
    2013, 29(6):1349-1354.
    [Abstract](1112) [HTML](0) [PDF 550.29 K](732)
    Abstract:
    The extraction of proanthocyanidins from skin of red locus seed were investigated by the single experiments and response surface methodology. The single experiments were used to explore the effects of different extractant, extractant concentration, different acid, pH, liquid-to-material ratio, extraction temperature, extraction time and extraction times on the yield of proanthocyanidins. The best extraction temperature, extraction time and extraction times were determined as 40 ℃, 90 min and one time, respectively. Then the extraction pH, liquid-to-material ratio, and acetone concentration were investigated through response surface experiment. Based on the regression analysis, the extractive conditions were optimized as follows: pH 2.6, liquid-to-material ratio 55 mL/g and acetone concentration 67%. The extraction rate of proanthocyanidins of this optimized procedure reached 9.57 %.
    38  Preparation of a Health Jelly Using Carrot and Honey
    JIA Juan
    2013, 29(6):1355-1358.
    [Abstract](1194) [HTML](0) [PDF 457.31 K](789)
    Abstract:
    The paper study production and process of carrot and honey health jelly,for the development to provide the reference by means of single factor, orthogonal experiment and the sensory quality as evaluation targets. The jelly made up of carrot juice as the main raw material, carrageenan, CMC-Na and agar gel as main colloid, and healthy and citric acid as accessories. The results showed that, carrot juice 30%, honey 12%, citric acid 0.28%, carrageenan 0.7%, CMC-Na 0.6% and agar gel 0.28%. The natural products of carrot and honey is taste smoothness, uniform color, sweet and sour moderate, have certain health care function, with the development of the future.
    39  Microwave-assisted Extraction of Polysaccharide from Phyllanthus urinaria
    TANG Kai YAN Jie LI Fu-lan
    2013, 29(6):1359-1361.
    [Abstract](951) [HTML](0) [PDF 303.40 K](679)
    Abstract:
    In this paper, micro-assisted extraction of polysaccharide from Phyllanthus urinaria was studied. The content of polysaccharide was determined by the method of phenol-sulfuric acid. Three main factors including extraction time, extraction power and ratio of solid to liquid were studied by orthogonal experiments. Results showed that extraction time was the most significant influential factor, followed by extraction power, and ratio of solid to liquid showed the minimum influence. The optimum extraction conditions were: extraction time 40 min, ratio of solid to liquid 1:20 and extraction power 400 W, under which the extraction yield of polysaccharide reached 7.9 wt%.
    40  Development of a New Matcha Graham Crackers
    ZHENG Li-na LIU Long
    2013, 29(6):1362-1364.
    [Abstract](943) [HTML](0) [PDF 417.98 K](720)
    Abstract:
    Using graham flour as main raw material and superfine powder as the auxiliary materials, a new matcha graham crackers was developed via adjustable powder, molding and baking. Through sensory evaluation and single factor experiment, the best formula was determined as: 125 g of whole wheat flour, 20 g of soybean oil, 0.5 g of salt, 40 g of sugar, 3g of matcha powder, 1.45 g of soda and 55 g of water, under which the product showed the best sensory quality.
    41  Development of LyciumBarbarum and Fruit Composite Sports Drink
    SUN Si-zhe
    2013, 29(6):1365-1369.
    [Abstract](1107) [HTML](0) [PDF 464.45 K](785)
    Abstract:
    Use LyciumBarbarum, fruit, sugar and citric acid as raw materials, through the single factor experiment and L9(34) process for producing LyciumBarbarum and fruit compound health beverage was studied by orthogonal test. Test results showed that the fruit juice, enzymatic hydrolysis conditions were: 0.05%, pectinase enzymolysis temperature 45 ℃, hydrolysis time 4H; wolfberry juice clarifying agent was: adding 0.07% chitosan; the best formula of wolfberry juice addition amount is 35%, calcium juice add 30%, 0.18% citric acid, sucrose adding 8% sodium chloride, 0.03%, vitamin C 0.01%, vitamin B1 0.004‰, vitamin B20.003 ‰. The product is given to the nutritionist and athletes after many experiments, and obtain the consistent high praise
    42  Identification of Immunogenic Proteins in Cronobacter sakazakii ATCC29544
    WANG Jian DU Xin-jun WANG Shuo
    2013, 29(6):1370-1373.
    [Abstract](1167) [HTML](0) [PDF 538.76 K](644)
    Abstract:
    Cronobacter spp. are emerging opportunistic pathogens. So far, little is known about the species’ immunogens and potential virulence factors. Immunogenic proteins play an important role in the pathogenicity of bacteria. In this study, the immunogenic proteins of C. sakazakii ATCC 29544 was identified using immunoproteomics. The polyclonal antibody was prepared using the formaldehyde-fixed C. sakazakii ATCC 29544 as antigen. Then the proteins of C. sakazakii ATCC 29544 were extracted using sucrose density gradient centrifugation and separated using two-dimensional electrophoresis. Western blot was used to detect the surface-exposed proteins and mass spectroscopy was used to identify them. 7 immunoreactive proteins were identified in this study.
    43  Determination of the Contents of Organic Acids of Raspberry
    SUN Jin-xu ZHU Hui-xia XIAO Dong-guang
    2013, 29(6):1374-1376.
    [Abstract](1075) [HTML](0) [PDF 434.50 K](749)
    Abstract:
    As an edible Chinese herb, raspberry could improve overall energy, vitality aphrodisiac and immune booster. A method for determining organic acids in Raspberry by HPLC was developed and the best HPLC determination conditions with a C18 column were determined as follows: column temperature 30 ℃, mobile phase 0.05 mmol H2SO4, flow rate 0.5 mL/min, detection wavelength 210 nm and injection volume 10 μL. Under the detection conditions. five organic acids could be successfully separated with good linear relationships (correlation coefficients above 0.9998). The precision RSD, repeatability RSD and recovery rates were of 0.30~0.90%, 0.42~3.69% and 97.86~102.35%, respectively. The method was convenient, rapid, accurate and applicable to determine organic acids in raspberry.
    44  Quantitative Determination of Multiple Elements in Leaf Mustard Using ICP-AES with Microwave Digestion
    LAI Zhi-hui ZHOU Jia-xin GUAN Yan-yan TAN Hui-ren WANG Xiao-chang WANG Su-juan ZHANG Yuan-he
    2013, 29(6):1377-1380.
    [Abstract](1014) [HTML](0) [PDF 524.12 K](744)
    Abstract:
    The contents of multiple elements(Al, As, Cd, Co, Cr, Cu, Mn, Ni and Pb)in six samples of leaf mustard tested by ICP-AES with microwave digestion. Under the optimal conditions, the recoveries of nine elements were in the range of 92.82~108.26%. The detection limit of ICP-AES was Al 0.15 mg/L, As 0.01 mg/L, Cd 0.005 mg/L, Co 0.01 mg/L, Cr 0.01 mg/L, Cu 0.03 mg/L, Mn 0.01 mg/L, Ni 0.01 mg/L, Pb 0.01 mg/L and the correlation coefficient was better than 0.999. It was concluded that elements(As, Cd, Cr and Pb)match the National Standard of Maximum levels of contaminants in food of China. The data can be applied to the quality control of trace elements in leaf mustard.
    45  Quantitative Determination of Five Nitrofuran Metabolites in Meat Meal using Solid Phase Extraction and LC-tandem MS
    HOU Xiang-chang Huang Jin-feng DU Zhi-feng XIAN Yan-ping GUO Xin-dong LUO Dong-hui WU Yu-luan LU Yu-jin
    2013, 29(6):1381-1385.
    [Abstract](1476) [HTML](0) [PDF 513.13 K](742)
    Abstract:
    A method for the simultaneous determination of five nitrofuran metabolites in meat meal by liquid chromatography tandem mass spectrometry (LC-MS/MS) was established, based on isotope dilution and solid phase extraction (SPE). The homogenized sample was spiked with isotope inter standards, hydrolyzed with HCl, derivatized with nitrobenzaldehyde, extracted with ethyl acetate, and cleaned up with HLB solid phase extraction cartridge. The analysis was carried out on C18 column by gradient elution with acetonitrile – ammonium acetate as mobile phase, and detected by MS/MS system with switch positive and negative electrospray ionization under multiple reaction monitoring (MRM) mode. The compounds was identified with retention time and ion ratio and quantified by the internal standard calibration curve of isotope dilution technique. The results showed that the high correlation coefficients (r>0.9990) of five targets were obtained within their respective linear ranges over dynamic range of 0.2~20 μg/L and the limits of detection (LOD, S/N=3) of five analytes were in the range of 0.05~0.2 μg/kg. The average recoveries were ranged from 80.33% to 103.24% for the five targets at three spiked levels with the relative standard deviations (RSD, n=6) were between 4.60% and 9.85%, and the inter-day RSD (n=5) was less than 10.3% at spiked levels of 0.5 μg/kg. The method was precise and sensitivity, suitable for quantitative and qualitative analysis of five nitrofuran metabolites in meat meal.
    46  Detection of Pork Freshness using a Novel Wirless Electronic Nose
    ZHOU Hong-biao ZHANG Yu-lin LI Shan XIA Yun
    2013, 29(6):1386-1389.
    [Abstract](1144) [HTML](0) [PDF 360.41 K](661)
    Abstract:
    The detecting possibility of a novel wineless electronic nose were explored for 5 different freshness pork samples, which was designed with the STM32 and CC2430. Data processing included extracting the steady-state response after smoothing, and using principal component analysis and probabilistic neural network to establish model for freshness recognition. The results showed that contribution rate of the first two principal components total reached 92.79%. Classification effect is obvious and the identification rate of probabilistic neural network model achieved 100%.
    47  Resuscitation and PMA-qPCR Detection of Staphylococcus aureus in the Viable but Non-culturable State
    TIAN Cong YU Yi-gang XIAO Xing-long WU Hui LAI Fu-rao YANG Xi-hong
    2013, 29(6):1390-1394.
    [Abstract](1514) [HTML](0) [PDF 605.07 K](1115)
    Abstract:
    In order to study the resuscitation of Staphylococcus aureus in the viable but non-culturable state (VBNC), three factors (temperature, salt concentrations and pH) were investigated to induce VBNC Staphylococcus aureus. Four different resuscitation methods were attempted and a new detection method using qPCR and PMA in combination with qPCR was established to detect Staphyloccocus aureus. The results showed that the VBNC samples could resuscitate in 48 h after disposed in 8% Tween80. In addition, PMA-qPCR was an effective method for detection of VBNC Staphyloccocus aureus, which efficiently overcome the shortcomings of plate counts.
    48  Determination of Polycyclic Aromatic Hydrocarbons in Fish by GC-QqQ–MS/MS
    LIU Bing HUO Lu-ge
    2013, 29(6):1395-1399.
    [Abstract](990) [HTML](0) [PDF 573.35 K](742)
    Abstract:
    A new GC-QqQ–MS/MS method was developed to determine the polycyclic aromatic hydrocarbons (PAHs) in fish. In this method, the samples were pretreated by ultrasonic extraction with hexane/dichloromethane and purified with hexane/acetone on florisil column, and then analyzed on a TR-5MS column. PAHs can be detected in the modes of electron impact ionization and selective reaction monitoring. The calibration curves had good linearity within 0.4~50 μg/kg for each pesticide (0.9999>R>0.9865). The limits of the detection ranged from 0.024 μg/kg to 0.06 μg/kg and the limits of quantitation ranged from 0.08 μg/kg to 0.2 μg/kg. The recoveries of PAHs varied from 68.5% to 106.3% with the relative standard deviations of 0.3~17.8% at the spiked levels 5, 10 and 50 μg/kg. It was satisfied with successfully determination of fish samples by this analytical approach.
    49  Determination of Nitrite by Fluorescence Spectrometry with Neutral Red included in β-Cyclodextrin
    TAN Shi-jie JIN Wen-ying HAI Hong
    2013, 29(6):1400-1402.
    [Abstract](1180) [HTML](0) [PDF 612.95 K](675)
    Abstract:
    An inclusion complex was prepared via synthesis of β-CD and neutral red envelope and the reaction of this complex with nitrite in hydrochloric acid was investigated. The results showed that, in hydrochloric acid, the inclusion complex can directly react with nitrite. The detection limit and relative standard deviation were within the rage of 0~120 μg/L, the NO2- content showed a linear relation with the fluorescence quenching spectrum. Common coexisting ions did not interfere with its determination. The proposed method can be used for water samples in the determination of nitrite with satisfactory results.
    50  Determination of Sodium Glutamate in Chicken Powder by Amino Acid Autoanalyzer
    ZHU Hui-mian FENG Zhi-qiang LUO Xiao-bao ZHOU Xing-qi
    2013, 29(6):1403-1405.
    [Abstract](1173) [HTML](0) [PDF 417.61 K](865)
    Abstract:
    This paper determined the content of sodium glutamate in chicken powder by amino acid autoanalyzer. The monosodium glutamate content in chicken powder was up to 0.27~0.32 g/g. This method had high degree of automation, reproducibility and accuracy. It was also easily operated with good reproducibility. Sample preparation was simple and direct.Its recovery can achieve 99.41~103.66% and the precision was 0.39 %.
    51  Comparison of Two Digestion Methods for Determination of Cadmium in Pinctada martensii by Flame Atomic Absorption Spectrometry
    WU Yu-lian YANG Jie LIAO Yan WU Xiao-ping
    2013, 29(6):1406-1408.
    [Abstract](1037) [HTML](0) [PDF 437.11 K](810)
    Abstract:
    Two kinds of sample digestion methods, high pressure airproof digestion and conventional wet digestion, were tested for determination of cadmium content in Pinctada martensii by flame atomic absorption spectrometry. The results showed that the cadmium contents in Pinctada martensii by high pressure digestion digestion and wet digestion were 60.96 mg/kg and 61.65 mg/kg, respectively. The RSD were less than 3% and the recovery by the two methods were 97.44~101.29% and 96.77~102.19%, respectively. Both of the two methods required relatively simple equipments and low cost. Digestion with high pressure airproof was more facile, convenient and needed less consumption of acid than wet digestion.
    52  Simultaneous Determination of Multiple Preservatives in Fruits and Vegetables by HPLC
    QI Yan HUANG Qiu-yan LIANG De-pei ZHOU Yong FAN Yao HUANG Cui-li
    2013, 29(6):1409-1412.
    [Abstract](4101) [HTML](0) [PDF 344.15 K](619)
    Abstract:
    A HPLC method was developed for the determination of multiple preservatives in fruits and vegetables, including ortho-phenyl phenol, thiabendazole, imazalil, carbendazim, cinnamaldehyde, 2-naphthol, and 2,4-dichlorobenzene oxygen ethanoic acid. Samples were extracted by alkaline ether. The extract was dissolved in methanol and finally injected into the HPLC system after the extraction solution was evaporated to dry on a rotary evaporator. The separations were effected by the gradient elution mode using a mobile phase composed of methanol-phosphate buffer (pH 6.8) at the flow of 0.8 mL/min. The eight preservatives were separated on an ODS-SP C18 column and then detected using a DAD detector with detection wavelength of 190~400 nm. The correlation coefficient of this method ranged from 0.9991 to 0.9997. The recoveries at spiked levels ranged from 93.4% to 97.4% and the relative standard deviations (RSD) ranged from 0.53 to 1.96. The results demonstrated that this method was simple, sensitive and accurate, which had a promising potential for practical applications in the determination of preservatives.
    53  Simultaneous Determination of 10 Synthetic Colors in Drink by Solid-Phase Extraction Coupled with HPLC/Photodiode Array Detection
    XIAN Rui-qing WANG Xiao-bing LI Qi-yan SHI Feng LI Jun-jie
    2013, 29(6):1413-1416.
    [Abstract](1284) [HTML](0) [PDF 455.60 K](884)
    Abstract:
    A method for simultaneous determination of 10 synthetic colors (new red, tartrazine, amaranth, indigotin, carmine red, sunset yellow, allura red, brilliant blue, acid orangeⅡ and erythrosine) in drink by solid-phase extraction coupled with high performance liquid chromatography-photodiode array detector was developed. The drink samples were cleaned up by Waters Oasis WAX and separated on an Aglient TC-C18 (5 μm, 250 mm×4.60 mm) column with methanol-0.02 mol/L ammonium acetate as mobile phase by gradient elution. The wave lengths for detection were from 210 nm to 650 nm. Qualitative analysis of synthetic colors was based on their retention times and ultraviolet absorption spectra. External standard method was applied for quantitative analysis. The results showed that the linear ranges of 10 synthetic colors were 1~20 μg/mL (r>0.999). The recoveries ranged from 90.2% to 101.6%with RSDs of 0.44~2.48% (n=6).The detection limits were in the range of 0.01~0.02 mg/kg. The method was simple, accurate and sensitive, which was suitable for the simultaneous determination of synthetic colors in drink.
    54  Determination of Aflatoxin B1 in Tartary Buckwheat Products by Enzyme-linked Immunosorbent Assay
    LAN Shan-shan CHEN Jin-yu SHAO Jin-liang YANG Fang LIU Hong-cheng
    2013, 29(6):1417-1419.
    [Abstract](1170) [HTML](0) [PDF 416.54 K](674)
    Abstract:
    The content of aflatoxin B1 in tartary buckwheat products was detected with ELISA kit, and the prevention and control measures of aflatoxin B1 were discussed. The aflatoxin B1 content of tartary buckwheat nutrition power was the least in the 4 kinds of produces analyzed, followed by tartary buckwheat flast dissolving tablets and tartary buckwheat vinegar. The aflatoxin B1 content of tartary buckwheat tea was the highest. The detection sensitivity was 0.1 μg/kg and the average spike recovery rate was 84.70~84.95%. The relative standard deviation was less than 5%. The results showed that the ELISA was accurate with good stability and reproducibility, so it can be applied to detect aflatoxin B1 in tartary buckwheat products widely.
    55  Effect of Yeast Fermentation on Volatile Components in Hot Pepper Sauce by HS-SPME-GC-MS
    TANG Xin XIA Yan-bin WU Can
    2013, 29(6):1420-1423.
    [Abstract](1279) [HTML](0) [PDF 550.08 K](725)
    Abstract:
    The volatile components of fermented hot pepper by lactobacillus and the mixture of lactobacillus and yeasts were analyzed by solid-phase microextration (SPME) and gas chromatography-mass spectrometry (GC-MS). Total 36 and 57 volatile components were identified in the fermented hot pepper by lactobacillus and the mixture of lactobacillus and yeasts, respectively.
    56  Determination of Trace Elements in Some Seasonings by Microwave Digestion-high Resolution Continuum Source Graphite Furnace Atomic Absorption Spectrophotometry
    ZHANG Jian-ping CHEN Shang-long LIU En-qi CHEN Hua-yun
    2013, 29(6):1424-1427.
    [Abstract](1070) [HTML](0) [PDF 529.46 K](647)
    Abstract:
    An effective method was developed for sequential rapid determination of trace elements in some seasonings by microwave digestion-high resolution continuum source graphite furnace atomic absorption spectrophotometry. The interferences were eliminated by adding the corresponding matrix modifiers. Results showed that the instrument detection limits of Cu, Cd, Pb, Mn and Al were 1.22 ng/mL, 0.077 ng/mL, 1.53 ng/mL, 0.614 ng/mL and 1.54 ng/mL, respectively by this method. The recoveries were 95.2~106.4%. The precision relative standard deviations were less than 5.22%. Therefore, this method was rapid, accurate, stable and less pollution, and had a high practical value. It provided scientific basis for sequential rapid determination of many trace elements in food.
    57  Analysis of Aroma Constituents in Persimmon Sweet Wine by Solid-phase Micro-extraction and GC-MS
    ZHANG Yuan ZHANG Jun-xiang LIU Ya-hui
    2013, 29(6):1428-1430.
    [Abstract](1024) [HTML](0) [PDF 431.94 K](749)
    Abstract:
    The flavoring compositions were extracted from persimmon sweet wine by solvent extraction and then analyzed by GC/MS. Their relative contents were determined by peak area normalization method. 32 volatile compounds were separated and identified, including lipid, alcohols, organic acids, aldehyde and a small amount of paraffin. The detection results showed that ethyl caprylate, phenylethyl alcohol, decanoic acid,ethyl ester, dimethyl phthalate, 2,3-butanediol had relatively higher contents than the others, probably being the main flavoring compositions for persimmon sweet wine.
    58  Enzyme-linked Immunosorbent Assay for the Detection of Zearalenone in Corn Oil
    MENG Wei-qin WU Yu-xiang TANG Shi-yun SHEN Zhi-qiang
    2013, 29(6):1431-1433.
    [Abstract](1132) [HTML](0) [PDF 414.12 K](1224)
    Abstract:
    To know the performance of the kit, indirect competitive enzyme-linked immunosorbent assay(ELISA) was used to detect the residues of zearalenone in the 3 kinds of corn oil. The results showed that the linear equation was: y=-2.2x+0.66, r=0.9974, IC50=0.29 μg/kg. Quantitation of zearalenone was linear from 0.05 μg/kg to 4.05 μg/kg. The sensitivity was 0.05 μg/kg. The recovery rates in the corn oil were more than 85%. The sensitivity, repeatability and specificity of the method were satisfactory and could meet the need of detecting the residues of zenralenone in corn oil.
    59  Determination of Rganophosphorus Pesticides in Strawberry by GC-SPE
    ZHAI Shuo-li ZHANG Xiu-feng
    2013, 29(6):1434-1436.
    [Abstract](1225) [HTML](0) [PDF 393.34 K](615)
    Abstract:
    A gas chromatography method was established for determination of 13 organophosphorus pesticides in strawberry. The organophosphorus pesticides were homogenated, extracted by acetonitrile and purified by solid phase extraction. The sample solution was analyzed by gas chromatography with Agilent-1701. Good separation for 13 organophosphorus pesticides was achieved using the established method, with correlation coefficients above 0.995. The detection limit was 0.0012~0.0082 mg/kg. The average recoveries of the 13 organophosphorus pesticides were 90.6~105.3% with RSDs of 1.28~6.30%. This method could meet the requirements for the organophosphorus pesticides in strawberry.
    60  Application of HACCP System in Production of Fruits and Vegetables Restructured Jerky
    ZHUANG Pei-rui CONG Yi-jie SUN Wei-zheng CHEN Chu-rui
    2013, 29(6):1437-1441.
    [Abstract](1092) [HTML](0) [PDF 558.18 K](830)
    Abstract:
    Fruits and vegetables restructured jerky is a kind of product combining fruits and vegetables with traditional jerky, aiming to change the characteristics of high fat and low dietary fiber traditional jerky. In order to improve the fruits and vegetables restructured jerky production management level, control the quality of products and enhance the safety of products, HACCP system was applied in analysis of remarkable harmful factors and key control point of production technology of fruits and vegetables restructured jerky. The results indicated that raw material (pork) acceptance check, adding of nitrate, dehydrating and detecting metal were key control points. Based on these, prevention and management measures were established.
    61  Research Progress of Cultivation of Oleaginous Micro-algae in Waste Water
    WANG Qin FU Jia-yin YING Yue LI Qi-xin HUANG Jia-ling
    2013, 29(6):1442-1446.
    [Abstract](1201) [HTML](0) [PDF 534.75 K](754)
    Abstract:
    The paper introduces the common species of oleaginous micro-algae in waste water and the important factors in cultivating oleaginous micro-algae in waste water, such as temperature, pH, percentage of nitrogen and phosphorus.The research development and technical problems of oleaginous micro-algae cultivation in waste water were also discussed.
    62  Biosorption of Heavy Metal Wastewater by Moulds
    LIN Hua-shan HUANG Wei QIU Yang
    2013, 29(6):1447-1454.
    [Abstract](1250) [HTML](0) [PDF 560.81 K](761)
    Abstract:
    Biosorption of heavy metals by moulds was regarded as a effective method for treating heavy metal wastewater. In this review, biosorption of heavy metal wastewater was reviewed, including analyzing the characteristics and hazards of heavy metals in wastewater pollution, advantages and disadvantages of wastewater treatment methods, the adsorption properties of different molds, the factors that affect fungal adsorption of heavy metals and the mechanism of fungal adsorption of heavy metals.
    63  Application of Microbial Preservation Technology in Fruit and Vegetable Preservation
    HUANG Ying-wei XU Cong MA Ke ZHANG Chang-yong PANG Xu LUO Shi HU Wen-feng
    2013, 29(6):1455-1458.
    [Abstract](1420) [HTML](0) [PDF 370.28 K](1683)
    Abstract:
    Development of postharvest technology of fruits and vegetables had significant economic and social benefits. However, with the widely attention to the food safety, new requirements for fruits and vegetables were put forward to fresh-keeping industry, which was natural, safety and nutritive. Microbial preservation is a kind of safe, nontoxic and effective bio-preservation technology, having wide application prospect. The application of antagonist preparation in the storage of fresh fruits and vegetables can control postharvest decay, reduce the adverse effect of chemical fungicides to people, and can prevent the resistance of fungi to fungicide. This article reviewed the progression in microbial preservation with the emphasis on the variety; operation way and action mechanism of the microorganism used in bio-preservation technology and analyzed the present situation and future development, which is expected to provide a theoretical reference for the development of biological preservatives for fruits and vegetable.
    64  Researches on Pharmacologic Effect of Essential Oil in Schizonepeta tenuifolia Briq
    QUAN Mei-ping
    2013, 29(6):1459-1462.
    [Abstract](1020) [HTML](0) [PDF 449.64 K](751)
    Abstract:
    Schizonepeta tenuifolia Briq essential oil has various pharmacological functions, showing enormous exploring potential for pharmaceuticals industry. The paper reviewed the current situation of pharmacological effects on essential oil in Schizonepeta tenuifolia Briq. The pharmacological mechanism of the essential oil in Schizonepeta tenuifolia Briq were discussed to provide references for development of essential oil in Schizonepeta tenuifolia Briq .
    65  Application of Biotechnology in Paper-making Reconstituted Tobacco Production
    LI Dan LIU Xi
    2013, 29(6):1463-1466.
    [Abstract](1095) [HTML](0) [PDF 440.84 K](823)
    Abstract:
    Production of reconstituted tobacco by biotechnology showed promising potential in tobacco field. This paper summarized the research progress of the application of biotechnology in reconstituted tobacco production. The application of microbial fermentation in the pretreatment of raw material and concentrated tobacco extracts were reviewed and the application of enzyme technology in the pretreatment of raw material, extraction process, concentrated tobacco extracts and paper coating were also discussed. Furthermoere, the prospect of this application was introduced to promote the application of biotechnology in the reconstituted tobacco production.

    Editor in chief:李琳

    Inauguration:现代食品科技

    International standard number:ISSN 1673-9078

    Unified domestic issue:CN 44-1620/TS

    Domestic postal code:46-349

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