Volume 28,Issue 6,2012 Table of Contents

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  • 1  Study of the Anti-obesity Function of Green Tea Extracts for Development of a Hypolipidemic Agents
    LIU An-jun GUO Dan- xiao LIU Hui- hui CHEN Hong-shuo ZHANG Hui ZHOU Shao-qian
    2012, 28(6):601-605.
    [Abstract](1425) [HTML](0) [PDF 771.24 K](816)
    Abstract:
    In this experiment, Fujian tea was treated by water extraction, alcohol extraction and alkaline extraction, giving crud polysaccharide, supernatant extracts, ethanol extracts and crude proteins. Using mice for the test animals by feeding them high-fat diet, high-fat obesity model was established. By setting model control and normal control groups, the weight-lose and lipid-lowering functions of four kinds of extracts were investigated. The results showed that, alcohol extract and the supernatant showed good effects on regulating blood lipids and weight control. Compared with model group, TG and body weight of the alcohol extract group decreased by 37.50% and 11.84%, respectively. TG and body weight of the supernatant extract group decreased by 35.42% and 7.32%, respectively. Compared with model group, crude protein and polysaccharide from Fujian tea showed lower lipid-lowering effec,. TC values of the mice in crude protein and polysaccharide groups decreased by 17.69% and 9.52%, respectively, and the LDL-C values of the mice decreased by 56.25% and 42.97%, respectively.In addition, HL activity in serum of the mice in ethanol extract group was significantly higher than that in the model group and the PL activity in serum of the mice in the supernatant group was significantly lower than that in the model group (P <0.01).
    2  Tea Tree (Melaleuca alternifolia) Oil induced Phenotypic and Functional Maturation of Murine Bone Marrow-derived Dendritic Cells
    TANG Yong-fu LI Ji-hua CHEN Jia-cui LIN Li-jing HAN Zhi-ping CAO Yu-po
    2012, 28(6):606-609.
    [Abstract](1080) [HTML](0) [PDF 714.81 K](744)
    Abstract:
    The effect of tea tree oil on the phenotypic and functional maturation of dendritic cell was investigated in vitro. Dendritic cells treated with tea tree oil (0.005 ?L/mL), were enlarged, suspended, and displayed typical stellate and aggregates like cells treated with LPS (positive controls). The levels of surface marker expression were monitored throughout the culture by flow cytometry using CD11c-PEcy7, I-Ab-FITC, CD86-FITC, and H-2Db-PE. Dendritic cells treated with tea tree oil expressed higher level of H-2Db (48.20%), costimulatory molecule I-Ab (6.60%) and CD86 (41.00%) with the cells gated by CD11c, being of 1.28, 3.40 and 1.28-fold higher for H-2Db, I-Ab and CD86, respectiely compared with untreated cells. It was also found that tea tree oil could enhance the dendritic cell to induce proliferation of T lymphocyte. And it was reduced the endocytic activity of dendritic cell by tea tree oil. These results showed that tea tree oil had significant immunoenhancing activity by inducing the phenotypic and functional maturation of dendritic cells.
    3  Effect of Ultrasonic Treatment on Viability of E. coli Cells
    LIU Li-yan ZHANG Xi-mei LI Lin LI Bing
    2012, 28(6):610-612.
    [Abstract](1141) [HTML](0) [PDF 387.14 K](648)
    Abstract:
    As a non-thermal physical technique, ultrasonic technology has many advantages, such as short time, low energy consumption, effective conservation of the food nutrients and natural characters of color, smell and taste. Therefore, it would have wide prospect of industrial application. Ultrasound is able to inactivate microbe cells through a number of physical, mechanical and chemical effects arising from acoustic cavitation. The aim of this study was to investigate the effect of ultrasound treatment at different conditions (intensity of ultrasound, treatment time, concentration of microbe solution) on cell viability of E. coli. Results showed that sterilization was greatly influenced by the ultrasonic treatment conditions.
    4  The Differences in Physical and Chemical Properties between Wenshi Partridge Chicken and Sanhuang Chicken
    LIAO Cai-hu ZHONG Rui-min HUANG Guo-qing XIAO Zi-jun RUI Han-ming
    2012, 28(6):613-616.
    [Abstract](964) [HTML](0) [PDF 574.59 K](693)
    Abstract:
    The differences in microcosmic and macroscopic structures between Wenshi partridge chicken and Sanhuang chicken were investigated. Nutritional evaluation showed that the nutritional value of Wenshi partridge chicken was lower than that of Sanhuang chicken. However, Wenshi partridge chicken contained high-quality protein according to the ideal model of FAO/WHO. The first limiting amino acids in the two kinds of muscles were Met and Cys according to both the amino acids score and chemistry score. The hardness, resilience, chewing character and recoverability of Sanhuang chicken were higher than those of Wenshi partridge chicken. The results indicated that contravariance ability and stability of myosin and actin in Sanhuang chicken were better than those in Wenshi chicken. But contravariance ability and stability of myogen in Sanhuang chicken were worse than thosed in Wenshi chicken.
    5  Research of Encapsulation Properties of Soy Protein Isolate
    LI Xin-rong TANG Chuan-he
    2012, 28(6):617-621.
    [Abstract](1370) [HTML](0) [PDF 823.26 K](750)
    Abstract:
    Emulsions of soy protein isolate (SPI) solutions and soya oil at core/wall ratios of 0.5-3.0 (m/m) with solid content of 10-20% were prepared by homogenization at 40MPa and 80MPa. The resulting emulsions were subsequently spray-dried at 160-200 ℃ inlet temperatures to produce microcapsule powders. Part of them were stored in relative humidity 75 % atmosphere at 25 ℃ and obtained samples when moisture sorption equilibrated. The droplet size distribution of fresh and reconstituted emulsions were determined, also moisture content, extractable oil, encapsulation efficiency (ME) and dissolution rate of powder samples were characterized, the morphology of powders with different core/wall ratios were established by SEM. The results indicated core/wall ratio had markedly influence on many properties of microcapsule powders. Furthermore, homogenization pressure, inlet temperature and solid content affected encapsulation properties of powder samples in different extent.
    6  Comparative Study on Foam Assessment Methods with Different Models of Dynamic Foam-collapsing Law
    ZHANG Li-da ZHAO Mou-ming ZHAO Hai-feng
    2012, 28(6):622-626.
    [Abstract](1067) [HTML](0) [PDF 579.20 K](692)
    Abstract:
    Sigma method and Constant method are two common methods for measuring foam stability. Foam stability was estimated by characteristic parameters of foam-collapsing models established for both methods. Results showed that two foam collapse models proposed by Sigma and Constant methods were both significant during measuring period (1-5min) in this study, while there were significant differences in prediction accuracy and evaluation results for foam stability. Foam collapse model proposed by Sigma method exhibited better prediction accuracy for the whole collapsing process. Pearson correlation analysis indicated that Sigma method correlated well with Chinese standard method. Moreover, results from relationships between composition and foam stability of beer samples further confirmed that proteins (MW>5,000) are decisive foam promoters in beer.
    7  Study on Rheological Behavior of Begonia Juices
    DING Shi-qiang
    2012, 28(6):627-629.
    [Abstract](1150) [HTML](0) [PDF 487.81 K](641)
    Abstract:
    LVDV-III + type rheometer was used to study the rheological characteristics of crabapple juice at different temperatures and Gabriel sugar content. Results showed that the apparent viscosity and shear stress of crabapple juice increased with the increase of shear rate. Along with the increase of the shear rate, a slightly upward trends in apparent viscosity was found. Crabapple juice showed the basic fluid rheological properties of inflation plastic. Fittly the experimental curves with Herschel-Bulkley equation showed that the R2 values was above 0.99.
    8  Study on Microstructure and Paste Viscosity of Acid-modified
    XIAO Xiang CAI Mang-quan ZHANG Xin-xin HUANG Li-xin
    2012, 28(6):630-633.
    [Abstract](1140) [HTML](0) [PDF 791.41 K](741)
    Abstract:
    The properties of acid & thermal modified mung bean powder were studied by polarization microscope, infrared spectrometer, differential scanning calorimetry (DSC) instrument, X-ray diffraction and Brabender viscosity instrument. The results indicated that starch granules were damaged in different degrees by different acid, and citrate starch appeared during this process. Compared with the original mung bean powder, DSC showed that the endothermic peak of modified mung bean starch moved forward, and the ΔH value was increased. X-ray diffraction analysis showed that the acid treatment reduced the crystalline structure of starch. Brabender paste viscosity curve showed that the samples had low viscosity which was similar to dextrin.
    9  Research on Effects of Malva Nut Gum on Quality of Minced Meat
    PI He-zhen RUI Han-ming ZHANG Li-yan
    2012, 28(6):634-638.
    [Abstract](914) [HTML](0) [PDF 616.34 K](719)
    Abstract:
    Malve nut gum formed by cortex of Sterculia lychnophora expansion in the water was investigated. The effect of malva nut gum on cooking loss, color, texture and sensory quality of minced meat were studied. The results showed that malva nut gum had a great influence on reducing the cooking loss of minced meat and improving meat products yield, which effected by the added amount and way of malva nut gum. The best texture and sensory quality of the minced meat were obtained by adding 0.2% dissolved malva nut gum. The addition of malva nut gum had some impact on the color of the meat by reduced brightness and increasing red and yellow degree. Texture analysis showed that malva nut gum reduced the hardness and chewing of the minced meat, while increased the flexibility the meat, in accordance with the results of sensory evaluation.
    10  Antioxidant Effects of BHA on Prepared Chicken Breast Strips during Cold Storage
    WANG Li LI Qing-tao NIU De-bao
    2012, 28(6):639-642.
    [Abstract](1205) [HTML](0) [PDF 453.68 K](654)
    Abstract:
    Butylated hydroxyanisole (BHA) with different concentrations was added to chicken breast strips. The chicken breast strips were stored at -18℃ and their peroxide value, acid value and thiobarbituric acid were measured to evaluate the antioxidation effects of BHA . The results showed that with the extension of storage time, acid value of the chicken breast strips firstly decreased and then increased, while TBA value continuously increased. No obvious trend in the peroxide value changes was found, which went up and down during storage. Significant decrease in the peroxide value (except for the sample stored for 45 days ) and TBA value of the chicken breast strips were found with the increase of BHA concentration, However, little effect of BHA on the acid value of the strips were found. The best concentration of BHA was determined as 0.15g/kg, with which the best antioxidant effect on the chicken bresast strips can be reached.
    11  Comparative Antioxidant Activity of the Extracts from the Rice Fermented by Aspergillus Oryzae Strains
    CHEN Zhou-tan JIN Yi-qiong WU Hui LAI Fu-rao
    2012, 28(6):643-646.
    [Abstract](1346) [HTML](0) [PDF 846.75 K](893)
    Abstract:
    The extracts from Aspergillus oryzae (A.oryzae), a common commercial fungus, are considered as natural antioxidants. To comparing the bioactivity of the extracts from fermented rice by four kinds of A.oryzae strains (A.oryzae M-1, A.oryzae MC-01, A.oryzae SP-01 and A. soji), the enzymatic activities of α-Amylase ,cellulose and β-Glucosidase in the extracts were analyzed and the DPPH radical scavenging abilities, reducing power, metal chelating activities, and total phenolic contents of the extracts were determined. A.soji was chosen as the best strain for further experiment, with which the fermented rice showed the highest phenolic compounds (9.60 mg/g).
    12  A Two-step Method for the Extraction of Oil from Xanthoceras Sorbifolia Bunge Seed
    YU Peng
    2012, 28(6):647-650.
    [Abstract](1127) [HTML](0) [PDF 533.65 K](841)
    Abstract:
    In this study, Xanthoceras sorbifolia seed oil was extracted by a two-step method . At the first step, Xanthoceras sorbifolia was roughly extracted at low temperature by oil press, and the bottom oil in the seed cake was 15.23%. At the second step, the seed cake was soaked in diethyl ether under the following conditions: solvent-material ratio of 5:1, extracting time of 6h, and extracting times of 4, under which the bottom oil was 1.34%. The contents of fatty acids in the final extracts were analysed by gas chromatograph. Results showed that the extracted oil contained oleic acid and linoleic acid and the total content of unsaturated fatty acid reached 92.12%.
    13  The Enzyme Characteristics of Endogenous Protease and Autolysis Technology of Tilapia Viscera
    RONG Jing JIANG Ai-min GUO Shan-guang WU Zhu-min WU De-bin
    2012, 28(6):651-654.
    [Abstract](1251) [HTML](0) [PDF 523.85 K](592)
    Abstract:
    The enzyme characteristics of endogenous protease were analyzed and the optimum autolysis conditions were determined by the orthogonal test. Result showed that the endogenous protease of Tilapia viscera was stable at pH of 5~11 and 40~50℃. The optimum conditions for autolysis of viscera were pH 8, temperature 45℃, raw/water materials 1:2 and reaction time 4 h, under which the nitrogen recovery ratio and degree of hydrolysis were 73.21% and 18.78% respectively.
    14  Graphical Approach for the Optimization of the Production of Clarified Lychee Juice by Pectinase Treatment
    LIANG Lin-zhen GUO Dan HU Zhuo-yan YU Kai YU Xiao-lin
    2012, 28(6):655-658.
    [Abstract](1129) [HTML](0) [PDF 594.42 K](889)
    Abstract:
    In order to obtain the clarified lychee juice, the effect of pectinase concentration (0.02%~0.06%), temperature (35~55 ℃) and time (1~3 h) on the yield and clarity of lychee juice was determined by using the response surface mythology (RSM). The results showed that the yield and clarity of juice were increased by enzymatic treatment. The optimum conditions for enzymatic treatment of lychee pulp obtained by RSM with graphical approach were 0.045%~0.05% (m/m) of pectinase concentration, 46~50℃ of temperature and 2.0~2.5 h of enzymatic time. The predicted values for juice yield and clarity were 90.40% and 91.10%, respectively, which was in accordance with the experimental values.
    15  Optimization of Sweetener compounds for Plum Fruit Cake Preparation
    GUO Zhuo-zhao CHEN Yu HE Jian-mei LI Ting YOU Xiao-mei LI Yuan-zhi
    2012, 28(6):659-661.
    [Abstract](1366) [HTML](0) [PDF 519.43 K](654)
    Abstract:
    Using the fresh Sanhua plum as the raw material, the processing technology and formula of Sanhua plum fruit cake were studied. Sensory evaluations such as color, texture and shape were taken as survey indicators. The formula of sweetening agents was optimized through orthogonal test as follows: neotame 2.5 g/kg, sodium cyclamate 6.0g/kg, saccharin sodium 1.51g/kg and stevioside 1.0g/kg. The product with the flavor and nutraiton of Sanhua plum could be prepared. The product had a tan color, sweet and sour taste and soft and elastic feeling.
    16  Optimization of Ultrasonic-assisted Enzymatic Extraction of Polysaccharide from Phellinus igniarius
    CHENG Wei QIN Jun-zhe DU Jun-guo ZHANG Chun-hua
    2012, 28(6):662-666.
    [Abstract](1280) [HTML](0) [PDF 717.79 K](684)
    Abstract:
    Ultrasonic-assisted extraction was applied to improve the cellulase-catalyzed extraction yield of polysaccharide from Phellinus igniarius.Based on the single factor experiments, four factors and three levels of response surface analysis method was adopted to optimize the extraction process based on central composite design principle. Infrared spectrometry was used to analyze the structural characteristics of the extracted polysaccharide from Phellinus igniarius. Results showed that the suitable extraction processing conditions were ultrasonic treatment temperature 54℃, pH 5.1, ultrasonic treatment time 39 min and ultrasonic treatment power 240w. Under the optimum conditions, the yield of Phellinus igniarius polysaccharide was up to 5.30%. Infrared spectroscopy indicated that the observed major functional groups in Phellinus igniarius polysaccharide were consistent with those extracted by hot water.
    17  Preparation of Navel Orange Jam by Fermentation of Lactic Acid Bacteria
    WEI Na XU Man
    2012, 28(6):667-669.
    [Abstract](1366) [HTML](0) [PDF 588.27 K](761)
    Abstract:
    The process conditions of a navel orange jam were investigated in this paper, in which navel orange and its peels were used as raw material and fermented by lactic acid bacteria. The influence on the quality of navel orange jam was investigated and the optimum conditions were obtained by orthogonal experiments as following: fermentation time 24 h, navel orange peel-orange ratio 1:4 and custer sugar dosage 40%. The navel orange jam was prepared with golden yellow color, good flowing property and a moderately sweet and acid flavor.
    18  Study of Brewing Technique for Reducing Methanol Content of Sugar Cane liquor
    WU Xiao-na KANG Fu-shuai YAN Rui-ming ZHOU Shi-shui
    2012, 28(6):670-671.
    [Abstract](1317) [HTML](0) [PDF 450.37 K](704)
    Abstract:
    A mutant of Saccharomyces cerevisiae, which was obtained by UV and MES mutation, was used for fermentation of sugar cane liquor to decrease the methanol content.The brewing techniques was optimized as: inoculums size 4.0x107cfu/mL, fermentation temperature 23℃ and fermentation time 6 days. Under the optimum fermenataion conditions, the methanol content of sugar cane liquor was 172.9 mg/L, which was 39% lower than that of the liquor brewed by original strain (284 mg/L).
    19  Application of Hurdle Technology on the Synthetic Preservation of Mochi
    WU Hao SHAO Hua-ping ZHU Yong WANG Jun WU Zhi-heng ZHANG Yu-jiong
    2012, 28(6):672-675.
    [Abstract](1295) [HTML](0) [PDF 586.47 K](751)
    Abstract:
    The results indicated that the treated product would be kept at a range of room temperature (15-20°C) for 60 days without degeneration and mildew when the following hurdle factors for raw material sesame were adopted (pressure 0.02 MPa, vapor-treatment time 30 minutes, roast temperature 150 ℃, roast time 45minutes, water activity 0.83,potassium sorbate 0.6 mg/kg ,sodium dehydroacetate 0.2 mg/kg and UV sterilization time 35 minute at 4 W/m2).
    20  Study on the Processing Technology of Lobster Mince Sauce
    BI Yan-hong GAO Hai-lin WANG Zhao-yu ZHAO Xi-rong ZHAO Li
    2012, 28(6):676-678.
    [Abstract](1206) [HTML](0) [PDF 471.86 K](664)
    Abstract:
    The seafood flavor sauce was prepared using lobster mince, peanut butter and fermented soya beans as the main raw material and adding pepper, salt, sugar, vinegar as the basic seasonings. Orthogonal test was employed to optimize the dosages of the ingredients and the processing conditions for lobster mince sauce. The best formula of the seasoning liquid contains salt 3% and pepper 1% with the ratio of the sugar to vinegar being of 5:3 (m/m),. And the optimal processing conditions for lobster mince sauce preparation were as follows: lobster mince 11%, seasoning liquid 27% and the ratio of peanut butter to fermented soya beans 2:8 (m/m).
    21  Study of Storage Conditions for Imported Soybeans in Squat Silos in South China
    LI Lin-jie MAI Jing-zhu
    2012, 28(6):679-683.
    [Abstract](1259) [HTML](0) [PDF 624.70 K](624)
    Abstract:
    The reserve capacity became increasing with the strategic needs in national grain reserves. However, the eco-climate conditions for grain reserve in South China, such as high temperature and humidity, were unfavorable for grain reserves. For the safe storage of soybean, the initial quality, storage characteristics in squat silos and storage conditions for soybean samples imported from Feb, 2008, were analyzed. The effects of three methods (traditional storage, high-concentration phosphine fumigation and temperature-control storage) on soybean reserve were discussed in order to develop a new method for the safe storage of imported soybean in squat silos in South China.
    22  Preliminary Study on Biodiesel Production via KOH Catalyzed Transesterification of Inferior Editable Oil
    HE Ping-wei DENG Fang-ming
    2012, 28(6):684-687.
    [Abstract](1168) [HTML](0) [PDF 379.64 K](647)
    Abstract:
    KOH catalyzed transesterification of inferior cooking oil was investigated for biodiesel production. The results showed that biodiesel production yield was increased at first and then decreased with increasing the reaction temperature, which reached the maximum value 92.6% at 65℃. The biodiesel yield can reached 89.2% when reaction time was of 1.5h. Further increasing the reaction time above 1.5 h led to only slightly increase in the biodiesel yield. Ratio of methanol to oil has a slight effect on biodiesel production and the optimal KOH dosage was 0.8%, with which 92.9% of biodiesel yield can be obtained.
    23  Study on Inhibition Methods of Acrylamide in Food
    LONG Xiao-tao HE Jia-rui YE Xue-li
    2012, 28(6):688-690.
    [Abstract](1031) [HTML](0) [PDF 438.51 K](826)
    Abstract:
    Recently, the study on the formation and inhibition of acrylamide has become a research hotspot in food safety. This paper briefly introduced the formation mechanism of acrylamide in food and summarized the inhibition methods on lab and industrial scale in detail. In addition, some problems in industrial application of the inhibition methods were discussed.
    24  Comparison of Nutrition Components in Mushroom in Different Batches
    ZHANG Xiao-shuang XU Xiao-fei ZHANG Bing-qing LUO Zhen CHEN Jian
    2012, 28(6):691-694.
    [Abstract](1233) [HTML](0) [PDF 554.56 K](968)
    Abstract:
    The components and contents of nutrients in mushroom in different batches were determined and compared, including moisture, ash, fat, crude protein, total sugar, amino acid composition, crude fiber, dry matter and polymer polysaccharide (HMWP), etc. The results showed a difference in nutritients of the mushroom of the four different batches. The mushroom in the first batch had higher contents of water and crude fiber but lower contents of dry matter and total sugar than those of other bathes. The contents of crude ash, dry matter,polymer polysaccharide,amino acids,essential amino acids of the mushroom in the second batch were higher than others but their crube protein were lower. High total sugar content but low polymer polysaccharide content were found in the samples of the third batch. For the mushroom of the forth batch, they showed high protein content but low crube riber content. Analysis of the nutrients in mushroom sample from the same origin and place showed that the pileus had high dry crude protein content, ash content, dry matter, and HMWP content, while the mushroom stipe had high fat, handle moisture, total sugar and fiber content. High content of essential amino acids were found in pileus .of the mushroom samples.
    25  Determination of 169 Pesticide Residues in Grains by Gas Chromatography Tandem Mass Spectrometry
    GUO Xin-dong XIAN Yan-ping LUO Hai-ying CHEN Yi-Guang HOU Xiang-chang LUO Dong-hui WU Yu-luan
    2012, 28(6):695-702.
    [Abstract](1379) [HTML](0) [PDF 827.51 K](806)
    Abstract:
    A method was developed for the simultaneous determination of 169 pesticides in grains by gas chromatography with tandem mass spectrometry (GC-MS/MS). The homogenized sample was extracted with acetonitrile, and then purified by QuEChERS, The targeted compounds were detected by GC-MS/MS system under the mode of multiple reaction monitoring (MRM). The results indicated that the limits of detection (LODs) of 169 pesticides were ranged from 0.3 to 13.0 μg/kg. The LOQs of the tested pesticides were between 1.0 and 40.0 μg/kg. In the linear range between 0.005 and 2.0 mg/L of each pesticide, the linear correlation coefficients were greater than 0.99. The average recoveries ranged from 65.3% to 143.6% and the RSDs of 97% pesticides were less than 20%. This method was suitable for the identification and quantification of multi-component pesticides in grains due to its simplicity, good purification and high sensitivity.
    26  Rapid Detection of Listeria monocytogenes in Milk Powder by Loop-mediated Isothermal Amplification
    LU Yan-xia MENG Zhao-xiang MA Xiao-yan WANG Yu ZHANG Xian-zhou ZHANG Wei
    2012, 28(6):703-706.
    [Abstract](1134) [HTML](0) [PDF 535.25 K](681)
    Abstract:
    Loop-mediated isothermal amplification (LAMP) is a novel DNA amplification technology with high specificity, efficiency and rapidity under isothermal conditions. In this research, a set of specific LAMP primers were designed to amplify the specific hlyA gene of Listeria monocytogenes by LAMP technology. The results showed that the detection sensitivity and detection limit of the LAMP method for purely cultured Listeria monocytogenes were 2.45×101 cfu/mL and 7.32×101cfu/mL, respectively. For other food pathogens, the target bands were not found . This method was suitable for rapid detection of Listeria monocytogenes in contaminated foods.
    27  Identification of Recycled Cooking Oil by FT-IR
    XU Hong-yong CHENG Lian WANG Dong-feng ZENG Shi-qiao YIN Zhao-ping XU Yu-cheng
    2012, 28(6):707-708.
    [Abstract](1533) [HTML](0) [PDF 469.21 K](666)
    Abstract:
    Fourier Transform Infrared Spectroscopy (FT-IR) was used to differentiate recycled cooking oil in this research. Results showed that, the recycling cooking oil had characteristic absorption peak at the wave number of 1711 cm-1, which was different from fresh edible vegetable oil. For the illegal cooking oil mixed with recycled cooking oil, the detection limit is 10%. GC-MS analysis showed that the characteristic peak of the recycled cooking oil samples represented carboxylic acid.
    28  Evaluation of Uncertainty in Determination of Cadmium in Meat Products by GFAAS
    CHEN Miao-rui YAN He SHI Lei
    2012, 28(6):709-711.
    [Abstract](923) [HTML](0) [PDF 447.11 K](602)
    Abstract:
    Based on GB/T 5009.15-2003, the content of cadmium can be determined with graphite furnace atomic absorption spectrometry. By estimation of uncertainty in measurement of cadmium evaluated, the sources of uncertainty in the detection process were analyzed and identified. After the assessment of uncertainty, the key factors affecting the test results were found. The research provided reliable theoretical basis for effectively improving the detection accuracy.
    29  Determination of Trace Elements in Fruit Wine by Inductively Coupled Plasma Mass Spectrometry
    LUO Xiao-yin YE Jia-rong GUO Xin-dong REN Lu-lu LUO Hai-ying WU Yu-luan
    2012, 28(6):712-715.
    [Abstract](1183) [HTML](0) [PDF 554.97 K](619)
    Abstract:
    A method was established to determination of 21 trace elements in fruit wine. After microwave digestion, the samples were determined directly by inductively coupled plasma mass spectrometry. Under the optimal conditions,the calibration curves for 21 metal elements were linear in the range of 0~10 μg/mL with correlation coefficients more than 0.999. The quantization limit of the method were ranged from 0.010 ng/g to 3.911 ng/g with RSD of 0.59%~4.16% (n=6).The mean recoveries of 21 metal elements from spiked samples at the spiking levels of 0.001~3000 μg/g were ranged from 89.7% to 112%. The proposed method is fast, simple, sensitive and accurate, which can meet the demand for determining 19 metal elements in fruit wine.
    30  Determination of Cis and Trans Fatty Acids in Formula Powders by GC
    LIU Dong-hao
    2012, 28(6):716-719.
    [Abstract](1320) [HTML](0) [PDF 624.28 K](697)
    Abstract:
    A internal standard method to determine cis- and trans- fatty acids in formula powders by gas chromatography was developed. Samples were methylesterized with acetyl chloride-MeOH,and analyzed by GC on a HP-88 column with FID as detector and triundecanoin as the internal standard. The results showed that cis- and trans- fatty acids in formula powders were well separated by this method. The recoveries, relative standard deviation and the detection limit of the GC method were 97.2%~104.6%, less than 3.5% and 5 μg/g, respectively.
    31  Comparative Study of the Methods for Determination of Total Sugar in Meat Products
    CHEN Na SHANG Yu QIU Yang YU Yi-gang XIAO Xing-long
    2012, 28(6):720-721.
    [Abstract](1157) [HTML](0) [PDF 331.72 K](713)
    Abstract:
    Total sugar content is an important quality indicator for meat products. The common UV spectrophotometry method and the microplate method were compared in this study. Results showed that total sμgar contents determined by microplate method were in accordance with those by UV spectrophotometry method. The microplate method needed fewer reagents, faster and had better repeatability than the UV spectrophotometry method.
    32  Determination of Sodium Cyclamate in Wine by GC-MS
    LIN Chao DUAN Jian-fa LU Yi-na
    2012, 28(6):722-724.
    [Abstract](1189) [HTML](0) [PDF 474.86 K](709)
    Abstract:
    The method for determination of sodium cyclamic in wines was improved by investigating the sample treatment procedure and adopting GC method. Sodium cyclamate showed good linear relations in the range from 1.0 mg/kg to 400 mg/kg, the recovery of the method was between 93.0% and 107.6%. The precision (RSD) was less than 4%. This method was suitable for determination of sodium cyclamate in wines with simple treatment procedure, high precision and accuracy.
    33  Estimation of Uncertainty in Spectrometry Measurements
    HU Wen-yan HONG Hong
    2012, 28(6):725-727.
    [Abstract](1353) [HTML](0) [PDF 412.49 K](829)
    Abstract:
    The factors in regression equation of measurement compose the sources of measurement uncertainty. The combined uncertainty and its components should be expressed in the form of standard uncertainty, using slope and intercept of the calibration equation as the independent parameters. Convenient function calculation is performed from partial derivative one by means of mathematical treatment. Coverage factor in expanded uncertainty, the commonly-used one in Europe, was adapt, which was suitable for the excellent instrument situation in China.

    Editor in chief:李琳

    Inauguration:现代食品科技

    International standard number:ISSN 1673-9078

    Unified domestic issue:CN 44-1620/TS

    Domestic postal code:46-349

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