Volume 28,Issue 1,2012 Table of Contents

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  • 1  Evaluation of Inactivation Efficiency of Shigella bacteriophage by Aerosol Spray
    ZHANG Hui WANG Ran BAO Hong-duo
    2012, 28(1):1-4.
    [Abstract](1201) [HTML](0) [PDF 364.45 K](602)
    Abstract:
    The efficiency of shigella phages mixture to inhibit pathogenic Shigella spp. by aerosol spray was investigated. Concentration of phages after aerosol spray was determined. Then, the host of Shigella flexneri 2a, Shigella dysenteriae and Shigella sonnei were sprayed with a total concentration of 3×105 cfu/mL. Phage cocktail were added thereafter (3×108 pfu/mL) immediately, and the number of Shigella spp. were counted 1 h, 2 h and 3 h respectively after spraying. The results showed that Shigella phages SF-2A、SD-11 and SS-92 could effectively inactivate their host strain, the host strain couldn’t be detected after spraying phages 3 h later. Therefore, the results suggest that phage cocktail, such as the phage of S. flexneri, S. dysenteriae and S. sonnei, could effectively reduce potential contamination of Shigella spp. in food production and processing.
    2  Study of properties of Soy Protein-Stabilized Emulsion Gels Induced by Microbial Transglutaminase
    YANG Miao TANG Chuan-he
    2012, 28(1):5-8.
    [Abstract](1101) [HTML](0) [PDF 466.92 K](662)
    Abstract:
    The properties and microstructures of MTGase induced SPI emulsion gels with different oil volume fractions (?=0.2~0.6) were studied. It was found that the water-holding capacity and G' progressively increased with oil fractions increasing. Confocal laser scanning microscope analyses indicated that the network microstructure of the formed emulsion gels varied with oil volume fractions. The networks became much stronger with oil volume increasing. Protein-coated oil droplets can act as “active fillers” in the emulsion gel formation.
    3  Effects of Different Heat Treatments on the Physicochemical Properties of Plantain Resistant Starch
    CHEN Ping-sheng HUANG Zhi-jun WANG Juan
    2012, 28(1):9-13.
    [Abstract](1248) [HTML](0) [PDF 711.10 K](691)
    Abstract:
    The effects of dry heating, moisture heating and microwave heating treatments on the physicochemical properties of plantain resistant starch (RS) were studied, which evaluated by RS retention rate, color, particle shape, iodine absorption curve, solubility and swelling ability, water-capacity of the treated plantain resistant starch. The results of experiments indicated that RS retention rates were reduced by the high temperature dry heating, moisture heating and microwave heating treatments; moisture heating and microwave heating treatments were not benefit for the color protection. The polarized light was weakened and the position of maximum absorption peaks of the iodine absorption curve was not changed by these three ways of heat treatments. Starch solubility was reduced by dry and moisture heating treatments, while increased by microwave heating treatments. Swelling ability was reduced by dry heating treatments, increased by microwave heating treatments, and was not changed significantly by moisture heating treatments.
    4  Study on Preservation of Fresh-cut Jackfruit by Chitosan Film
    JIANG Qiu-huan YE Sheng-quan YE Chun-hai LV Qing-fang HAN Rui
    2012, 28(1):14-17.
    [Abstract](1324) [HTML](0) [PDF 342.33 K](1035)
    Abstract:
    The effect of chitosan film on the preservation of fresh-cut jackfruit was studied. The change of reducing sugar content, total sugar content and water content was studied, and the effect of chitosan film on the preservation of dry and wet jackfruit was analyzed. It illustrated that the variation of reducing sugar treated jackfruit was less than that of untreated ones under the condition of storage at 3±1 ℃ for 15 days. Reducing sugar contents of wet and dry decreased by 3.58% and 3.81%, respectively, while that of uncoated group reduced by 4.77%. Water content of wet and dry jackfruit coated was lowest in the sixth day. Water content of uncoated group was 65.96%, and that of wet and dry jackfruit was respectively 72.06% and 73.81%. And total acid content of these respectively decreased by 0.13% and 0.15%. Total acid content of uncoated group was reduced by 0.23%. Chitosan coating could delay the ripening of jackfruit, and the preservation of wet jackfruit coated with chitosan was better than that of dry jackfruit.
    5  Study of the Stability of Purple Corn Pigment
    XIAO Xiang WANG Li-ya
    2012, 28(1):18-22.
    [Abstract](1159) [HTML](0) [PDF 444.53 K](679)
    Abstract:
    The effects of pH value, preservatives, metal ions, redox, and food ingredients on the stability of purple corn anthocyanin were studied. And the different color, preservation rate, and degradation mechanism of the purple corn anthocyanin under different pH value were also investigated. The results indicated that: the color of the purple corn anthocyanin changed from red to blue with the increase of pH value. The anthocyanin showed high stability when mixed with sodium benzoate or sorbic acid. Different metal ions, different redox, different food ingredients had different influence in absorbance of the purple corn anthocyanin, but in varying degrees. The optimum pH value for storage of anthocyanin was 2 to 3 and the anthocyanin degradation followed first-order reaction kinetics.
    6  Changes of Colors in Prunus mume during Smoking Processing
    DING Chao LI Bian-sheng
    2012, 28(1):23-26.
    [Abstract](1162) [HTML](0) [PDF 340.56 K](776)
    Abstract:
    Non-enzymatic browning in Prunus mume due to smoking process was investigated. Browning index, colorimetric parameters, content in reducing sugar, total sugar, amino acids and hydroxymethylfurfural (5-HMF) were measured to estimate the extent of nonenzymatic browning in the smoking process. The results showed that browning index, color difference and 5-HMF content gradually increased with the smoking time, while the content of reducing sugar, total sugar and amino acids decreased.
    7  Response of Physiology of Actinidia chinensis Planch to Selenium
    LI Ya-min JI Hai-ning
    2012, 28(1):27-29.
    [Abstract](1228) [HTML](0) [PDF 320.41 K](702)
    Abstract:
    The effects of selenium on physiological and biochemical characteristics in Actinidia chinensis Planch leaves were studied. The plants were cultivated with different selenium (Se) concentrations (0.15, 0.3, 0.5 mg/kg Na2SeO3). When the flower buds appeared, the leaves were harvested for measurement of chlorophyll content and activities of four antioxidant enzymes. The results showed that selenium added to Actinidia chinensis Planch leaves increased chlorophyll content, the activity of glutathione peroxidase (GPx), superoxide dismutase (SOD) and peroxidase (POD), while decreased the activities of superoxide dismutase (SOD) and peroxidase (POD) when Selenium was over 0.5 mg/kg. Therefore, treatments with suitable concentration of selenium could enhance the antioxidant abilities of Actinidia chinensis Planch leaves.
    8  The Influence of Glycerin on Static Rheological Properties of SPI Solution
    KONG Jie-jing YE Xiao-wen YE Jun XIONG Jian
    2012, 28(1):30-32.
    [Abstract](1395) [HTML](0) [PDF 417.44 K](860)
    Abstract:
    The influence of glycerin on static rheological properties and the rehological behaviors of SPI solutions were investigated. The change of shear viscosity of SPI with concentration and temperature after adding glycerin was discussed, specially comparing with SPI control solution. The results have indicated that SPI/glycerin solutions presented a typical behavior of pseudoplastic fluid, and glycerin not only reduced the viscosity and weakened pseudoplastic behavior of SPI but also improve its thermal stability.
    9  In Vitro Antioxidant Activity of Feruloyl Arabinose from Maize Bran
    LIN Qi-ling WEN Qi-biao OU Shi-yi LAI Fu-rao
    2012, 28(1):33-38.
    [Abstract](1149) [HTML](0) [PDF 352.70 K](683)
    Abstract:
    In this study feruloylated oligosaccharides (FOs) was released from maize bran by hydrochloric acid hydrolysis, and feruloyl arabinose (F-Ara) was obtained by D301 macroporous resin chromatography followed by polyamide resin purification from FOs. After structural identification, the antioxidant activity of F-Ara was evaluated in vitro by DPPH and superoxide radical scavenging activity assay and reducing power assay. F-Ara exhibited a good radical-scavenging activity in vitro when compared to standard antioxidants such as ferulic acid (FA), BHA and L-ascorbic acid (VC). The radical-scavenging activity depended on the concentration and increases with increasing dose of sample. The highest reducing value of F-Ara compared with FA and VC at a dosage of 0.6 mmol/L indicated that F-Ara had a considerable reducing power. The present study suggested that F-Ara can be a natural and efficient antioxidant used in food, medicine and cosmetic.
    10  Preparation of Hairtail Essence by Maillard Reaction
    LIU An-jun LIU Ya-jing ZHENG Jie LIU Xue-qin GUO Dan-xiao LIU Jia-qi
    2012, 28(1):39-42.
    [Abstract](1139) [HTML](0) [PDF 341.86 K](705)
    Abstract:
    Using hydrolyzate of hairtail waste, hairtail essence was made by Maillard reaction with some amino acid and reducing Sugar. By Single factor and orthogonal experiment, the best reaction conditions were determined as follows: Xylose-glucose mass ratio1:2, sugar dosage 8%, Glycine-glutamate mass ratio 1:3, and amino acid dosage 2%, pH 5.0, temperature 110 ℃, and the time 30 min, under which the reaction solution was Reddish-brown, clarify transparency, rich in flavor of the typical characteristics of hairtail.
    11  Screening of Camellia Saponin-Degradating Strains and Optimization of the Cultivation Conditions
    ZHOU Yue GUO Hua WANG Xiao-rong
    2012, 28(1):43-46.
    [Abstract](910) [HTML](0) [PDF 321.15 K](636)
    Abstract:
    Penicillium was screened for the degradation of camellia saponin . The optimal condition for Penicillium culture and camellia saponin degradation was determined through single factor test and L9(34)orthogonal test. The best culture conditions were: culture temperature 28 ℃, inoculation dose 12%, pH 4.5, and Penicillium cultivating time 5 days. Under these condition degradation rate of camellia saponin could reached 83.11%.
    12  Lipase Immobilization with a New Crosslinking Reagent Tris(hydroxymethyl)phosphine
    LIANG Xin-xin WEI Dong
    2012, 28(1):47-51.
    [Abstract](1168) [HTML](0) [PDF 391.75 K](615)
    Abstract:
    In the present work, tris(hydroxymethyl)phosphine (THP) was used as a new crosslinking reagent in lipase immobilization on cotton towel with adsorbent polyethylenimine (PEI). The optimal conditions of immobilization by single factors test were obtained as follows: 0.5 mL of PEI (3.37 mg/mL, pH 7.0) for each 0.2 g cotton towe, 2ml of THP [0.10% (V/V)], immobilization temperature 40 ℃ and crosslinking reaction time 20 min. THP-immobilized lipase can catalyze interesterification of vinyl butyric acid and pentanol. The conversion rate in 1h achieved 92.1%, and the activity of immobilized lipase was 15.80 U/g. Compared with the GA-immobilized lipase, THP-immobilized lipase had higher reusability, thermal stability and activity, indicating this is a low cost and practical newly method for lipase immobilization.
    13  Optimization of Ultrasonic Extraction Process of Polyphenols from Sweet Corncobs
    LAI Fu-rao LI Zhen WU Hui LI Xiao-feng
    2012, 28(1):52-55.
    [Abstract](1009) [HTML](0) [PDF 399.39 K](681)
    Abstract:
    Single factor and orthogonal array design experiments were used to optimize the ultrasonic extraction conditions of polyphenol from sweet corncobs. The total polyphenol content was determined by the Folin-Ciocalteu assay. Results showed that the optimum extraction conditions were as follows: 45 min ultrasonic treatment at 200W power for extracting the raw material with a 15-fold volume of 80% aqueous ethanol solution at 40 ℃. The yield of polyphenol was (2.61±0.09)%, under the optimum condition, and DPPH radical scavenging activity under this condition reached (70.05±0.17)%.
    14  Study on Subcritical Extraction of Camellia Seed Oil
    GUAN Xiao-sheng CHE Ke XIAO Su-yao CHEN Xue-xiang HUANG Yi-cheng CAO Yong WEN Xiu-jun
    2012, 28(1):56-60.
    [Abstract](1190) [HTML](0) [PDF 437.08 K](798)
    Abstract:
    Camellia seeds were chosen as raw material in this study,using the new subcritical extraction and molecular distillation to study the optimum conditions of high quality camellia oil. Using oil yield, color and benzopyrene content as the indexes, the optimum conditions of subcritical extraction were: bulk density of raw materials as 0.7 kg/L, extracting time as 50 mins, extracting pressure as 0.5 MPa and the extracting temperature as 45 ℃.The extraction efficiencies of camellia oil was up to 99.12%, with an increase of 15% than traditional method. Refining of molecular distillation conditions were determined: the temperature of 160 ℃, vacuum of 1.0~2.0 Pa, and the resulted acid value decreased by 70.87%, to 0.22 mg/g. Subcritical extraction of camellia oil benzo(?)pyrene were not detected. The studies process has fast, environmentally friendly, low cost and simple technology characteristics. The quality of the camellia seeds meets the national standards for oil.
    15  Application of Whey Protein in No-addtives Yoghurt
    LIAO Wen-yan SU Mi-ya ZHOU Jie XU Zhi-yuan SHEN ling WANG hao
    2012, 28(1):61-65.
    [Abstract](1508) [HTML](0) [PDF 443.90 K](829)
    Abstract:
    In this experiment, whey protein YO-5011 was added to produce yoghurt without stabilization additives. Different quantity of whey protein was added to observe its effect on the yoghurt fermentation end point, as well as the titratable acidity, viscosity, STS, WHC during post fermentation and self-life time. The result showed the effect of the addition quantity of whey protein on the fermentation end point was not significant. The more whey protein added, the more rapidly titratable acidity increased. Whey protein enhanced the yoghurt stability during self-life time and improves STS and WHC which guaranteed the final product quality. Besides, combined with the result of additional sensory testing, the product taste and stability was optimized with a 3~4% whey protein addition.
    16  Degradation of Phytic Acid in Rapeseed Processing Water by Yeast Fermentation
    HUANG Yao-wu ZHOU Jian-ping
    2012, 28(1):66-68.
    [Abstract](1120) [HTML](0) [PDF 393.08 K](559)
    Abstract:
    There is plenty of process water after rapeseed oil extraction by aqueous enzymatic method. The process water contains water-soluble proteins, carbohydrates and anti nutritional factors. In order to depredate the anti-nutrition-phytic acid, the yeast fermentation was conducted and the optimal culture condition was determined by orthogonal experiment. The result showed that phytic acid degradation rate could reach to 87% under optimal conditions which is cultural temperate at 32 ℃,pH 7, and cultural time 36 h. The research provided references for rapeseed processing water recycling or discharging
    17  Preparation of Rice Beverage by Mixed Fermentation Lactic Acid Bacteria
    YU Wen-wen WU Hui GUO Ya-peng MIN Tian LI Jie PAN Bo-liang
    2012, 28(1):69-73.
    [Abstract](1108) [HTML](0) [PDF 399.52 K](654)
    Abstract:
    This essay is aimed at producing rice beverage using rice and malt as main raw materials. The rice beverage can be produced with the following processing steps: mixing, elatinization, smashing, mashing, fermentation by mixed lactic acid bacteria, and addition of apple juice, sugar and CMC. The optimum ratio of rice and water 1:10 before saccharification. For saccharification, the optimum wort concentration and the saccharifying enzyme dosage were 30% of raw materials and 1% of liquid fermentation, respectively. For pro-fermentation, the best ratio of Streptococcus thermophillus and Lactobacillus bulgaricus, the amount of mother fermented liquid, fermentation time and temperature were 3:2, 1%~3%, 70~80 hours and 37 ℃, respectively. For post-fermentation, the best temperature was 4~5 ℃ and the time was 15~20 days. Under the optimized conditions, the rice beverage was produced with abundant nutrition and excellent flavor.
    18  Study of Hexane Diacid-crosslinked Broken Rice Starch
    GOU Lin WANG Ze-nan GUO Zhi-jian JIANG Yan XU Chun-ze
    2012, 28(1):74-76.
    [Abstract](1152) [HTML](0) [PDF 337.98 K](639)
    Abstract:
    The preparation of hexane diacid-crosslinked broken rice starch was studied using broken rice starch as raw materials using hexane diacid as crosslinking regents. Single factor test and the following orthogonal experimental design were used to obtain the best preparation technology. The amount of hexane-diacid, pH, reaction time and reaction temperature were 0.5% (m/m), 9, 1.0 h and 60 ℃, respectively. Under these conditions, the preparation of hexane diacid-crosslinked starch’s crosslinking degree is up to 0.75 mL.
    19  Production of a Modified-starch Tobacco Humectants Additive
    WU Jin-ming ZHUO Hao-lian
    2012, 28(1):77-81.
    [Abstract](1091) [HTML](0) [PDF 320.38 K](547)
    Abstract:
    Strong hydrophilic group was introduced in the starch molecular by chemical reaction, greatly increasing water absorption properties and holding capacity. Reaction conditions for the production were determined as 10% of hydrogen peroxide amount, 60 ℃ of the reaction temperature, 3h of reaction time. The moistening performance of modified-starch humectants and traditional humectants were compared. The result showed that the moistening performance of modified-starch humectants was better than that of the traditional one.
    20  Preparation of an Inhalational Jelly containing Fermented Milk
    HUANG Xiao-qing ZHONG Xiu-juan
    2012, 28(1):82-85.
    [Abstract](1112) [HTML](0) [PDF 342.26 K](646)
    Abstract:
    Fresh milk or powdered milk was fermented by stain ABT-5. Then the fermented milk was mixed with 12% white sugar, 0.18% citric acid, 0.8% composited coagulator (0.20% CMC-Na+0.05% pectin+0.20% carrageenan+0.35% konjac glucomannan+0.05% xanthan gum+0.05% locust bean gum), and 0.2% emusifer. After homogenized by 20 MPa and sterilization, a novel inhalational jelly containing fermented milk was obtained. The product had good stability, sterilization and high nutrition value.
    21  Lipase-catalyzed synthesis of monocylglycerol rich in α-Linolenic acid from Linseed oil by response surface methodology
    LIU Yan-feng HUANG Huihua
    2012, 28(1):86-90.
    [Abstract](978) [HTML](0) [PDF 508.46 K](770)
    Abstract:
    Lipase-catalyzed synthesis of monoacylglycerol production between Linseed oil and glycerol in a solvent system by Lipozyme 435 was studied by response surface methodology. The result showed the optimal parameters were as follows: substrate molar ratio of Linseed oil to glycerol 1:3, the t-butyl alcohol ratio 39.66% (oil to t-butyl alcohol, m/V), enzyme dosage 6.01wt%, reaction temperature 56.11℃ and reaction time 10.48h. Under the optimal conditions, monoacylglycerol content could reach 71.1% in the reaction product, and the content of α-linolenic acid reached 51.36% in the monoacylglycerol.
    22  Preparation Process of Slowly Digestible Starch from Wheat
    ZHANG Bao-yue CHEN Li-jie SONG Xian-liang
    2012, 28(1):91-95.
    [Abstract](962) [HTML](0) [PDF 432.82 K](643)
    Abstract:
    This experiment studies enzyme modified technology and moist heat-cooling technology to make slowly digestible starch and determine its optimized technological conditions. Experimental results showed that the concentration of starch was 20%, the pullulanase concentration was 8ASPU/ml, the enzyme reaction time was four hours, the storage temperature was 4 ℃, the storage time was two day, and SDS obtained the highest rate of 52.8%. And heat treatment temperature was 120 ℃, the heat treatment time was one hour, storage time was 18 hours for the best program in heat-cooling technology, SDS obtained the highest rate of 36.5%.
    23  Study of Manufacturing Technique of the Crisp Nougat
    YU Zu-chun
    2012, 28(1):96-98.
    [Abstract](1044) [HTML](0) [PDF 288.62 K](642)
    Abstract:
    Effects of some influential factor on effect quality were studied and then an orthogonal test design was applied to obtain the optimal process conditions. The best manufacturing conditions are as follows: sugar/glucose syrup 5:5, boiled temperature 145 ℃, 2% dosage of egg Albumen, and tired time25 min.
    24  Microbiological Safety and Control Technology of Cooked Meat Products in China
    LIU Xue-ming FANG Shao-qin TANG Dao-bang WU Ji-jun
    2012, 28(1):99-103.
    [Abstract](1162) [HTML](0) [PDF 349.42 K](763)
    Abstract:
    Microbilogical safety of cooked or ready-to-eat meat products in China and its causes were reviewed and the control technology was suggested. According to Chinese national standard of cooked meat products, “Hygienic Standard for Cooked Meat Products”, 8.62%~88.30% of cooked meat products were up to the hygienic standard, while 10.20%~65.00%, 9.47%~83.33% and 0.00%~9.30% of cooked meat products were not up to the standards of colony-forming units, coliform MPN and pathogens, respectively. The low microbiological qualification ratio of cooked meat products was mainly due to the poor production condition, less hygienic knowledge, insufficient surveillance, etc. The quality of cooked meat products could be improved by raising the production threshold of cooked meat products, enhancing hygienic education for employees, intensifying hygienic surveillance and increasing packaged ready-to-eat meat products.
    25  Analysis on Scophthatmus maximus Amino Acids at Different Developing Stages
    WANG Cai-li GUO Xiao-hua YUAN De-shun Teng Yu
    2012, 28(1):104-107.
    [Abstract](986) [HTML](0) [PDF 325.50 K](655)
    Abstract:
    Amino acids compositions of Scophthatmus maximus at different developing stages were analyzed. Results showed that Scophthatmus maximus were high-quality edible proteins in that their EAA/TAA 47~48% and EAA/NEAA 87~93% (CV about 2%) were fit to amino acid ideal pattern (EAA/TAA about 40%、EAA/NEAA>60%) issued by FAO/WHO. EAA composition patterns of Scophthatmus maximus varied at different developing stages, indicating that special feed should be made according to Scophthatmus maximus different developing stages. The study can optimize feed prescription of Scophthatmus maximus at different developing stages by providing reliable data support and scientific reference.
    26  The Edible Safety of Combined Use of Coenzyme Q10 and Vitamin E
    LI Yu LIANG Yi-ying FENG Xiao-wen
    2012, 28(1):108-114.
    [Abstract](1541) [HTML](0) [PDF 532.30 K](668)
    Abstract:
    The stability and toxicity of the combined use of natural vitamin E and coenzyme Q10 were investigated to confirm the safety of the use of the mixture of Coenzyme Q10 and Vitamin E as a health food ingredient. Five different formulations containing natural vitamin E soft capsules and coenzyme Q10 was analyzed by high-performance liquid chromatography and their toxicological safety was assessed. Results showed that, in various formulations, no significant changes were found in contents of coenzyme Q10 and α-tocopherol. No new peak was found when extending the detection time to 3 times of the retention time. The presence of excipients did not show any impact on its stability. No reaction of Coenzyme Q10 and natural vitamin E was found in a 24-month period. Toxicological evaluation showed that the combination of natural vitamin E and coenzyme Q10 can be applied safely in food production.
    27  Determination of 18 Phthalic Acid Esters Compounds in Oily Foodstuffs by Atmospheric Pressure Chemical Ionization (APCI) Liquid Chromatography Tandem Mass Spectrometry
    ZHU Wei-xia YANG Ji-zhou YUAN Ping LIU Ya-feng ZHANG Ju-zhou SUN Zhuan-lian
    2012, 28(1):115-118.
    [Abstract](1064) [HTML](0) [PDF 345.01 K](681)
    Abstract:
    A quick confirmative method was developed for determining 18 phthalic acid esters (PAEs) in oily foodstuffs by liquid chromatography tandem quadrupole mass spectrometry (LC-MS/MS), basing on the ministry of health announcement about 17 illegal PAEs in foodstuffs. The oil sample was extracted with acetonitrile. After mixed with water, n-hexane was selected as the solvent of liquid-liquid extraction. The extracts were concentrated to dry and methanol was used to dissolve residues. The separation was carried out on C18 column with the isocratic and gradient elution. The detection of 18 analytes was achieved in the positive atmosphere pressure chemical ionization (APCI) and multi-reaction monitoring (MRM) mode. With the optimized condition, the limits of quantification (LOQ) of the 18 PEAs analytes were 0.05-0.5 mg/kg. The overall recovery varies from 53.4% to 107.9%, and the relative standard deviations (RSD) were between 7.2% and 24.6%. Real sample including vegetable oil, shortening oil, chili sauce, bean sauce, and biscuit were determined 18 PAEs residues with this method and compared with the standard method. The results showed that the method was simple, accurate and suitable for the determination of 18 PAEs in oily foodstuffs.
    28  Dual-wavelength Colorimetric Method for Measuring Amylose and Amylopectin Contents of Potato Starch
    ZENG Fan-kui ZHAO Xin ZHOU Tian-hong LIU Gang
    2012, 28(1):119-122.
    [Abstract](1253) [HTML](0) [PDF 373.25 K](1053)
    Abstract:
    Dual-wavelength colorimetry was developed to determine amylose and amylopectin contents after the starch was isolated from potato. By staining with I2-KI solution, amylose displayed a blue color, amylopectin displays a violet color. Absorbancies at 535 and 570nm were used for amylose content measurement, absorbancies at 555 and 760 nm are used for amylopectin content measurement. The XIN DAPING potato starch (from Dingxi, Gansu province) contained 21.20 % of amylase and 78.80 % of amylopectin. The detection results can be affected by the sample dissolution and the reaction time of starch and I2-KI solution.
    29  Determination of Glycerides by HPLC
    ZHONG Nan-jing LI Lin LI Bing LU Qi-yu LIU Guo-qin
    2012, 28(1):123-126.
    [Abstract](1461) [HTML](0) [PDF 375.08 K](1040)
    Abstract:
    In this paper, reverse phase HPLC-ELSD and normal phase HPLC-RI were used to determine the products from glycerolysis of soybean oil. The results indicated that the two methods were suitable for determination of the relative content of monoacylglycerols (MAG), diacylglycerols (DAG) and triacylglycerols (TAG). Oleic acid glycerides were determined by reverse phase HPLC-UV, the standard curves of 1-monoolein (1-O), 1,3-diolein (1,3-OO) and triolein (OOO) were established, and the correlation coefficients were higher than 0.99.
    30  Application of Loop-mediated Isothermal Amplification Assay for Detection Peanut allergy
    LI Yi-ming WANG Yu-ke YE Yu-xing YA He SHI Lei
    2012, 28(1):127-130.
    [Abstract](1006) [HTML](0) [PDF 476.89 K](632)
    Abstract:
    More and more attention is paid to peanut allergies, so the detection of peanut allergens is becoming increasingly important. In most case, antigen-antibody method and PCR method are mostly used in the detection of peanut allergen. However, antigen-antibody method takes longer, and the PCR method requires expensive equipment. In this report, the loop-mediated isothermal amplification (LAMP) method was developed to detect the gene of peanuts which is related to allergens producing. This method is rapid, simple and sensitive, which can provide convenience for peanut allergens detection. The establishment of the LAMP method has great significance and it provide a great convenience for food safety testing.

    Editor in chief:李琳

    Inauguration:现代食品科技

    International standard number:ISSN 1673-9078

    Unified domestic issue:CN 44-1620/TS

    Domestic postal code:46-349

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