Volume 24,Issue 2,2008 Table of Contents

1 Protective Effect of Collagen Polypeptide-chromium Chelate in Alloxan-treated HL 7702 Hepatocytes

LIU An-jun , CHEN Ying , ZHANG Guo-rong

2008, 24(2):101-104.

[Abstract](90) [HTML](0) [PDF 12.98 M](372)

Abstract:
To study the protective effect of collagen polypeptide-chromium chelate (CPCC) in HL 7702 hepatocytes treated by alloxan and the possible mechanism. Cells were divided into control group, alloxan-treated group, CPCC group and alloxan+CPCC group. Cell viability, contents of reactive oxygen species (ROS) and superoxide radical (O2.-) , activities of SOD and GSH-Px and MDA content were analyzed. The protective effect of CPCC in H2O2-treated hepatices was also determined. Results shows that Cell viability was significantly increased by CPCC, while the contents of ROS, O2.- and MDA and the activities of SOD and GSH-Px in alloxan-treated hepatocyte were decreased. CPCC had a protective effect in alloxan-treated HL 7702 hepatocytes, acting as an antioxidant.

2 Identification of Microbial Species in Pu-erh Tea with Different Storage Time

FANG Xiang , CHEN Dong , LI Jing-jing , ZHAO Chao-yi , LI Bin , HUANG Guo-zhi , CHEN Zhong-zheng

2008, 24(2):105-108.

[Abstract](96) [HTML](0) [PDF 384.17 K](253)

Abstract:
Eight kinds of microbial species were found in eight samples of Pu-erh tea with different storage time, which were Aspergillus niger, Penicillium chrysogenum, Rhizopus sp., Trichoderma sp., Aspergullus glaucus, Saccharomyces sp., Actinomycetales and Bacterium.

3 Differences in the Physicochemical Characteristcs between the muscles of Ctenopharyngodon idellus C. et V and Ctenopharyngodon idellus

ZHU Zhi-wei , LI Bian-sheng , RUAN Zheng , MENG Ming-yan , ZENG Qing-xiao

2008, 24(2):109-112.

[Abstract](135) [HTML](0) [PDF 185.43 K](357)

Abstract:
The differences in general chemical components, TPA value, muscle protein components, amino acid components and nutritional evaluation between muscles of Ctenopharyngodon idellus C. et V and Ctenopharyngodon idellus were investigated. The results showed that water content in Ctenopharyngodon idellus C. et V muscle was lower than that in Ctenopharyngodon idellus muscle, while the contents of protein, lipid and ash in the former were higher than those in the latter. Besides, the contents of sarcoplasmic protein, myofibrillar protein and stroma protein in Ctenopharyngodon idellus C. et V muscle were higher than those in Ctenopharyngodon idellus C. et V. And the hardness, chewiness, resilience and adhesiveness of Ctenopharyngodon idellus C. et V muscle were higher than those of Ctenopharyngodon idellus. Besides, the amino acids contents in Ctenopharyngodon idellus C. et V muscle were higher than those in Ctenopharyngodon idellus except Tyr, Arg and Ser. According to amino acid score, the first limiting amino acids in the two kinds of muscles were Met and Cys, while the second limiting amino acid was Val in Ctenopharyngodon idellus C. et V and Leu in Ctenopharyngodon idellus, respectively.

4 Production of Polyglutamic Acid by Fermentation of Soybean Meal

ZHANG Ning , KUANG Chi

2008, 24(2):113-114.

[Abstract](105) [HTML](0) [PDF 323.00 K](227)

Abstract:
Microbial Polyglutamic acid (PGA), a new polymer, can be widely applied in medicine and food industries. In this paper, production of PGA by fermentation of soybean meal was studied. The optimal ratio of soybean meal to water, sugar content, culture temperature, time and inoculum in the medium were 1:4, sugar dosage 2%, 40 ℃, 24 h and inoculum size 0.16 mL/g, respectively, under which the yield of PGA reached 4.81%.

5 Study of the Enzymatic Hydrolysis of Tilapa Meat by Different Protease

ZHAO Shan-shan , ZHU Zhi-wei , ZENG Qing-xiao , CHAO Dai-xiu

2008, 24(2):115-119.

[Abstract](97) [HTML](0) [PDF 16.82 M](341)

Abstract:
The enzymatic hydrolysis of tilaplia meat by papain, Alcalase 2.4 L, Protamex, Neutrase 1.5 MG and Flavourzyme 500 MG were studied. The nitrogen content of free amino acid (Cn), content of soluble peptides (Cp) and the recovery rate of protein were analyzed during the hydrolysis. Results showed that the highest values of Cn (4.25 mg/ml after 12 h) and Cp (39.82 mg/ml after 5 h) were abstained with Flavourzyme 500 Mg and papain respectively. And the highest recovery of protein was shown as 44.74% using Protamex for 12 h. Thus, Flavourzyme 500 MG and Protamex were suitable for obtaining the hydrolysate containing high nitrogen content of free amino acid, while Papain and Alcalase 2.4 L can be used for high content of soluble peptides.

6 Study on the Anti-oxidation and Bacteriostasis of Forsythia suspense Essential Oil

GU Ren-yong , LI You-ji , FU Wei-chang

2008, 24(2):120-122.

[Abstract](163) [HTML](0) [PDF 1.44 M](375)

Abstract:
The diameter of inhibition cycle of the Forsythia suspense essential oil, extracted by Supercritical carbon dioxide extraction（SCDE）technique, on various bacteria was studied. The effects of temperature and pH value on the bacteriostasis of this essential oil were also investigated. The results showed that, the inhibition cycle diameters of this essential oil on mold, yeast, G+ and G- bacteria were range from 12.5 to 26.0 mm, i.e. the essential oil had strong bacteriostatic functions on the tested bacteria. The lower the pH value, the higher the bacteriostatic of the essential oil. Heating treatment (121 ℃ for 15 minutes) had little effect on its bacteriostasis. Results also showed that the oil had anti-oxidation effect.

7 Effects of Nitrogen Sources on Monascus Yellow Pigment Production by Monascus Mutant

ZHOU Bo , PU Yue-wu , ZHU Ming-jun , LIANG Shi-zhong

2008, 24(2):123-127.

[Abstract](92) [HTML](0) [PDF 17.95 M](335)

Abstract:
In this paper, the nitrogen sources of culture media for Monascus mutant MYM2 were optimized. Effects of some inorganic nitrogen sources and organic nitrogen sources on Monascus yellow pigment production were investigated. Results showed that the best inorganic nitrogen source, organic nitrogen source for the production of yellow pigment by MYM2 were 1% of corn steep liquor and ammonium chloride, with which the yields of Monascus yellow pigment productions reached 84.12 U/mL and 72.17 U/mL respectively. cultured in the medium containing the mixture of the corn steep liquor (1%) and ammonium chloride (1%), MYM2 could produce 96.51 U/mL of the Monascus yellow pigment .Both intracellular and extracellular colour hue were > 3.

8 Effect of β-CD on the Thermal Stability of Isoflavone Aglycones in Soybean Milk

WANG Feng , HUANG HUI-hua

2008, 24(2):128-130.

[Abstract](77) [HTML](0) [PDF 521.33 K](376)

Abstract:
The effects of β-CycloDextrin (β-CD) on three aglycones (daidzein, glycitein and genistein) in soybean milk were investigated by HPLC. The best mobile phase was the mixture of methanol and 5% acetic acid glacial (40:60, V/V). It was found that adding 1% of β-CD into soybean milks could increase the thermal stabilities of the three aglycones. The contents of daidzein, glycitein and genistein in soybean milk containing β-CD were 8.89%, 13.81% and 9.75% respectively higher than those in soybean milks without of β-CD after incubated at 95 ℃ for 10 min, were 9.59%, 8.77% and 2.45% respectively higher than those in soybean milks without of β-CD after incubated at 121 ℃ for 1min and were 8.90%, 11.92%, and 6.69% respectively higher than those in soybean milks without of β-CD after incubated at140 ℃ for 10 s.

9 Separation of Glutathione from Waste Beer Yeast by Macroporous Resin

QIU Yan-ling , HU Jing , MIAO Jing-feng , LIANG Liang

2008, 24(2):131-133.

[Abstract](95) [HTML](0) [PDF 9.94 M](395)

Abstract:
Macroporous resin D001 was adopted for separation of GSH from waste beer yeast. The result indicated that the optimum concentration of phosphate buffer, pH values for adsorption and elution and the elution rate were 0.02 mol/L, 4.5, 7.5, and 2.9 mL/min, respectively. Under those conditions, the purity of the crude white GSH was 28.47% analyzed by HPLC, and the average yield was 20.03%.

10 Isolation and Characteristics of Yeasts from Xiaogan Fengwo Rice Wine Starter

WANG Xiao-hong , XU Kang , ZHAO San , CHENFu-sheng , ZHANG Xiu-yan

2008, 24(2):134-137.

[Abstract](80) [HTML](0) [PDF 1.39 M](322)

Abstract:
In this paper, 16 yeast strains were isolated from Fengwo rice starter and purified by PDA and malty solid mediums. Morphological observation and identification with the API 20C AUX system showed that, among the 16 yeast strains, 9 strains were of Saccharomyces cerevisiae and 7 strains were of Candida. The fermentability of these yeasts was also studied.

11 Optimization of Protoplast Preparation from Spores of Streptoverticilluim mobaraense

BAO Ying-ling , PAN Li

2008, 24(2):138-142.

[Abstract](210) [HTML](0) [PDF 17.14 M](441)

Abstract:
The preparations of protoplast of Streptoverticilluim mobaraense were studied. Results showed that 4% glycine containd in culture medium were propitious to release of protolast. The optimal preparation conditions were as follows: enzymatic temperature 41 ℃, lysozyme 2.5 mg/mL, enzymatic time 4 h. Under those conditions, the product of the generation rate and regeneration rate reached 24%.

12 Quality Evaluation of Sinkiang Glycyrrhiza and Extraction of Glycyrrhiza Polysaccharide

ZHANG Ze-sheng , SHI Shen , BAI Xin , YANG Hai-yan

2008, 24(2):143-146.

[Abstract](85) [HTML](0) [PDF 8.76 M](432)

Abstract:
In this study, the characteristics of different Sinkiang Glycyrrhizas were compared by determining the contents of glycyrrhiza acid and glycyrrhiza polysaccharide. And the extraction conditions of glycyrrhiza polysaccharide were investigated via single factor and orthogonal experiments. The results showed that the side branches of planted Glycyrrhiza contained 2.9% glycyrrhiza acid and 5.0% glycyrrhiza polysaccharide, which were higher than those in the trunks (glycyrrhiza acid 2.2% and glycyrrhiza polysaccharide 3.5%). In the wild glycyrrhiza, the highest contents of glycyrrhiza acid and glycyrrhiza polysaccharide were 2.4% and 3.3%, respectively. Ten years old wild glycyrrhiza contained 2.4% glycyrrhiza acid and glycyrrhiza polysaccharide. For the extraction of glycyrrhiza polysaccharide, the most suitable ratio of solid to liquid, extracting temperature, time and times were found to be 1:30, 90 ℃, 0.5 h and twice, respectively.

13 Processing Technology of Yogurt Beverage with Mango Juice

DENG Kai-ye , DAI Xue-qun

2008, 24(2):147-149.

[Abstract](118) [HTML](0) [PDF 286.45 K](409)

Abstract:
The processing technology of a fermented yogurt beverage with mango flavor was investigated. The filtrated mango juice was heated at 80~90 ℃ for 1min to prevent browning of the juice. The optimum amounts of mango juice addition, milk powder addition and sugar addition, inoculum size, fermentation temperature and time were 30%, 5%, 8%, 5%, 41 ℃ and 4.5 h, respectively. Under those conditions, the achieved beverage tasted smooth and had special flavors of mango and milk.

14 Preparation of Cookie using Maize Core Powder

JIANG Li-qin , ZHANG Xiao-lin , HU Jun-li , DING Yu-xia , HE Ting

2008, 24(2):150-152.

[Abstract](123) [HTML](0) [PDF 244.73 K](332)

Abstract:
A new kind of cookie made by maize core powder (MCP) was studied. The best ratio of wheat powder to MCP, ratios of the main material to butter, sugar and egg were 7:3, 1:0.65, 1:0.25 and 1:0.35, respectively. The achieved cookies were rich in dietary fiber and had nice flavor.

15 Optimization of Culture Conditions of a White-Rot Fungus for Laccase Production by Response Surface Design

FAN Wen-xia , CAI You-hua , LIU Xue-ming , XIAO Geng-sheng , CHEN Wei-dong

2008, 24(2):153-156.

[Abstract](141) [HTML](0) [PDF 1.04 M](347)

Abstract:
The fermentation conditions of a white-rot fungus Coriolus hirsutus for laccase production were optimized by single factor experiment and response surface design. The results showed that the optimal inoculum size, amount of broth in flask and shaking rate were 8.2%, 30.8% and 141r/min, respectively.

16 Methods for Extracting GSH from Saccharomyces cerevisiae

CHEN Na , QIAO Chang-sheng , HU Yu-xia , TAN Zhi-lei , JIA Shi-ru

2008, 24(2):157-160.

[Abstract](93) [HTML](0) [PDF 15.48 M](329)

Abstract:
Four methods for extracting GSH from Saccharomyces cerevisiae were studied in this paper. It was found that GSH could be efficiently extracted by boiling water extraction, microwave assistant extraction, ultrasonic disruption and high-pressure homogenization. Under the optimized extraction conditions, the GSH concentrations by high-pressure homogenization, boiling water extraction, ultrasonic disruption and microwave assistant extraction were 3.975 mg/g, 3.830 mg/g, 3.823 mg/g and 3.593 mg/g, respectively. And the extraction rates by the above-mentioned four methods were 99.87%, 96.23%, and 96.12% and 90.28%, respectively. The “green” high-pressure homogenization was the most suitable one for industrial production among the examined extraction methods.

17 Purification and Identification of Polysaccharides from Averrhoa caramhola L.

ZHU Yin-ling , TAN Zhu-jun

2008, 24(2):161-163.

[Abstract](86) [HTML](0) [PDF 382.28 K](266)

Abstract:
Crude Averrhoa caramhola L. polysaccharides, obtained by extraction with hot water and precipitation with ethyl alcohol, were purified by gel filtration chromatography after removing the free proteins and then identified by ultraviolet spectrum scanning and IR spectroscope. The results showed that the average molecular weight of the Averrhoa caramhola L. polysaccharides was 4.373×104. And the polysaccharides consisted of β-D-mannose pyranose, α-D-Galactopyranose and α-D-mannose pyranose.

18 Extraction of Total Flavonoids from Chinese Pine Pollen

YANG Zhi-yan , YIN Shu-hua , BAI Ming , GUO Li-mei

2008, 24(2):164-166.

[Abstract](81) [HTML](0) [PDF 3.45 M](294)

Abstract:
The extraction conditions of total flavonoids from Chinese pine pollen were studied in this paper. The optimum reflux extraction conditions were as follows: temperature of 70 ℃, ethanol content of 60% and ratio of solid to liquid of 10:1. In these conditions, the content of flavonoids in the extracts was 1.41%.

19 Extraction of Glycoprotein from Squid Viscera and its Immunomodulation

ZHANG Jian-she , LEI Xiao-ling

2008, 24(2):167-169.

[Abstract](81) [HTML](0) [PDF 322.09 K](228)

Abstract:
The General nutrients of squid viscera were analyzed and the extraction of glycoprotein from squid viscera by NaCl solution was investigated. The immunoloregulation effect of the glycoprotein was also researched. Results showed that squid viscera contained high contents of proteins. The optimum extraction conditions were as follows: ratio of solid to liquid of 1:6, extraction time of 60 min, NaCl content of 3% and temperature of 80℃. Our experiments also confirmed the extracted glycoprotein possessed immunomodulation activity.

20 Preparation of Low-sugar Compound Jam with Balsam Pear and Carrot

FU Xiao-ping , WU Ming , GAO Bin , HU Qing-hu

2008, 24(2):170-172.

[Abstract](79) [HTML](0) [PDF 270.87 K](256)

Abstract:
A compound jam was produced using balsam Pear and carrot as the raw materials and the processing technologies were determined through orthogonal experiments. The product had nice flavor and color with rich nutrition and 40% of soluble solid.

21 A Survey of Dietary Intake and Nutritional status of College Students

YU Hong-peng , CHEN Zhong-wei , WU Ke-gang , CHAI Xiang-hua

2008, 24(2):173-176.

[Abstract](99) [HTML](0) [PDF 291.51 K](611)

Abstract:
The dietary intake and nutritional status of college students were investigate. Food frequency questionnaire (FFQ) and weighted food record (WFR) were used to investigate the students’ food structure and nutrition intakes. Results showed that the results showed that 93.4% of college students’ diet nutrition was imbalanced. The intakes of vegetable, fruit, bean product, egg, seafood, and milk were less than the suggested values in Dietary Guideline and Balance Diet Pagoda for Chinese Residents. So we pay attention to the effects of the changes in food consumption and dietary pattern on the health of college students.

22 Analysis and Detection of Sodium Hydrosulphite in Flour

ZHOU David , YANG Yan-li , LIN Li , ZHOU Yin-fei , SUN Ji-hong

2008, 24(2):177-179.

[Abstract](92) [HTML](0) [PDF 283.29 K](303)

Abstract:
The content of sodium hydrosulphite, an illegal chemical additive in flour, was detected by Ion Chromatography after oxidized by KMnO4. Results showed that the content of sodium hydrosulphite in flour was 500~2000 mg/kg. The ranges of recovery rate and RSD were 93.04%~117.42% and 0.57%~2.25%, respectively. This simple and accurate method was suitable for the sodium hydrosulphite determination in flour.

23 Determination of Polysaccharides in Phyllostachys pubescens Leaves by Phenol-vitriolic Colorimetry

ZHOU Yue-bin , WANG Wei , ZHOU Xiang-rong

2008, 24(2):180-183.

[Abstract](80) [HTML](0) [PDF 7.28 M](372)

Abstract:
The content of total polysaccharides in Phyllostachys pubescens leaves by phenol- vitriolic colorimetry were studied. Results showed that this method was credible and reproducible and the linear concentration range was within 20~100 μg/mL (R2=0.9993) at the best detection wavelength of 490 nm, recurrence standard deviation was 0.0055 and the rate of recycle was 99.22%.

24 Examination and Enumeration of Pseudomonas in Meat and Meat Products

NIE Yan-yan , LIU Dong-hong , HUANG Yu-feng , WU Yu-luan , HOU Xiang-chang , CAI Wei-hong

2008, 24(2):184-187.

[Abstract](92) [HTML](0) [PDF 355.16 K](257)

Abstract:
Pseudomonas mainly accounts for the rot of meat and meat products. Examination and enumeration of Pseudomonas in the refrigerated, frozen and fresh meat and different meat products were studied here and the corresponding methods were established.

25 Determination of 5-Hydroxymethylfurfural in Soy by HPLC

WANG Miao-fei , ZHANG Shui-hua , GUO Xin-dong , DU Zhi-feng , WU Yu-luan , WANG Yong-hua

2008, 24(2):188-190.

[Abstract](104) [HTML](0) [PDF 5.65 M](385)

Abstract:
A new method for the determination of 5-hydroxymethylfurfural by high performance liquid chromatography in soy was developed. After extracted by ethyl acetate, the samples were analyzed on a C18 column at 284 nm using a mixture of methanol and water (5:95, v/v) as the mobile phase . Within the linear range of 0.5~20.0 mg/L, the method was fast and accurate with the average recoveries rate and relative standard deviation of 82.4%~86.9% and 3.2%~5.1% , respectively. The minimum determination limit was 0.1 mg/L.

26 Determination of Sugar Degrees of the Black Sugar

YU Yu-juan , LIANG Xiao-wen , CAO Wei-qiang

2008, 24(2):191-192.

[Abstract](81) [HTML](0) [PDF 220.38 K](256)

Abstract:
The sugar degrees of the black sugar containing impurity were determined by Polartronic NIR W2 at 882 nm. The method was fast and precise with great precision of 99.9%.

27 Nutrigenomics and its Applications

TANG Yu-qian , LIN Ying , LIANG Shi-zhong , ZHU Yi , YE Yan-rui , LIU Yun-duan

2008, 24(2):193-198.

[Abstract](117) [HTML](0) [PDF 301.60 K](333)

Abstract:
With the development and application of genomics and bioinformatics, nutrigenomics emerges and becomes a hotspot in nutrition research. Nutrigenomics mainly researches on the effects of nutrients and phytochemicals on the transcription and translation of human genes and their metabolism mechanisms, applying high-throughput genomics technology in nutrition research, especially the molecular mechanism and requirement of nutrients, the personal recipe and animal product, etc. In this article, the current researches and the trends of the applications of nutrigenomics were discussed.

28 An Exploration of the Teaching Reform of the Postgraduate Course of “Functional Material of Carbohydrate”

HE Xiao-wei , HUANG Qiang

2008, 24(2):199-200.

[Abstract](77) [HTML](0) [PDF 146.17 K](235)

Abstract:
Based on the characteristics of the education and special course for postgraduate, the methods and ideas of teaching reform for postgraduate course “Functional material of carbohydrate” were summarized, including the teaching contents, methods, materials, course ware design, etc. It can provide references for the teaching of related postgraduate courses.

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Volume 24,Issue 2,2008 Table of Contents