Volume 24,Issue 1,2008 Table of Contents

1 Study on the Stability of Acidic Fishmilk Compound Protein Beverage

CHEN Wen-fen , CUI Chun , XIAO Ru-wu , WEI Yi-wen , HUANG Hui-yi , FU Guo-qiang , ZHAO Mou-ming

2008, 24(1):1-4.

[Abstract](113) [HTML](0) [PDF 6.67 M](327)

Abstract:
In this paper, we studied the effects of five emulsifiers on the stability of acidic fishmilk beverage by measure several factors, including centrifugal settling rate, precipitation rate of milk fat, particle size distribution, etc. The result showed that the best stabilizers were 4 g/L of CMC-Na and pectin, which can effectively prevent disposition of protein in fishmilk beverage. The particle size and its distribution correlated with the stability of acidic fishmilk beverage. The smaller the particle size and the higher the density of the distribution, the more stable the system. So the particle size distribution can be used to judge the stability of acidic fishmilk beverage.

2 Investigation of the Drying Characteristics of Pineapple Powder

CHANG Hong , LI Yuan-zhi , YANG Xuan-xuan

2008, 24(1):5-7.

[Abstract](94) [HTML](0) [PDF 1.60 M](355)

Abstract:
The pineapple was treated by warm air drying and vacuum drying, and its drying characteristics were studied in this paper. Results showed that, for the warm air drying of pineapple, the optimum technologic parameters were as follows: the temperature of 70 ℃, wind speed of 2.5 m/s and the material amounts of 60 g. For vacuum drying, the optimum vacuum, temperature and the material amount were 0.08 MPa, 60 ℃ and 60 g, respectively. The powder treated by vacuum drying had better characteristics and higher Vc retention than that by warm air drying.

3 Influence of Microwave Irradiation on the Rheological Property of Waxy Corn Starch Paste

MU Yan , CHEN Ling , LI Lin , LI Xiao-xi , ZOU Fang-jian

2008, 24(1):8-10.

[Abstract](167) [HTML](0) [PDF 5.12 M](414)

Abstract:
The modification of waxy corn starch paste by microwave irradiation was studied. The effects of temperature, microwave power and irradiation time on the rheological property of waxy corn starch paste were investigated using a rotary viscometer. Results showed that the rheological property could be greatly affected by microwave irradiation. The treatment of microwave irradiation decreased the concentration and viscosity of the paste, changing the paste from a pseudoplastic fluid to a Newtonian fluid. This change tendency became obvious by improving the temperature and the power as well as prolonging the irradiation time.

4 Construction and Indentification of the Class1 Integrons Expression Vector

DUN Bo-ying , ZENG Bing-bing , YAN He , SHI Lei

2008, 24(1):11-13.

[Abstract](103) [HTML](0) [PDF 1.44 M](331)

Abstract:
Class1 integrase gene was amplified by PCR, then the amplicon was connected to pET19b vector after cutting by Ned1 and BamH1. The recombinant plasmids were induced to express integrase in E.coli BL21(DE3), and the integrase was identified by SDS-PAGE and Western Blot. The results showed that the integrase can be expressed in E.coli BL21(DE3).

5 Study on Antioxidant Activity of Crude Extract from Chestnut Flower

WU Xue-hui , ZHANG Xi-mei , LI Ting-qun , LIN Yan , MEI Can-hui

2008, 24(1):14-16.

[Abstract](163) [HTML](0) [PDF 389.24 K](314)

Abstract:
The effective component was extracted from chestnut flower by three methods, including microwave, ultrasound and conventional reflux extraction. The antioxidant activities of the crude extracts were studied through free radical system and nitrite system. The results showed that all of the crude extracts of chestnut flower obtained by the three extraction methods had good antioxidant activity, which could effectively eliminate DPPH• and •OH with the maximal elimination-rate being of 92.8% and 54.3%, respectively. Besides, the three kinds of extracts could eliminate the nitrite and block the synthesis of nitrosamine with the highest elimination rate and blocking rate being of 72.9% and 89.9%, respectively, while they had little effect on the elimination of O2-•.

6 Effect of Emulsification Stabilizer on the Stability of Milk Tea

YIN Bo-xing , XU Xiao-gang

2008, 24(1):17-19.

[Abstract](196) [HTML](0) [PDF 269.26 K](298)

Abstract:
The effects of emulsifier, hydrocolloid and salt on the precipitation rate, fat separation rate and viscosity of milk tea were studied. Results showed that the best ratio of distilled monoglyceride to succinylated monoglyceride, the carrageenan dosage, sodium citrate and the emulsifier content were 3:2, 0.30 g/L ,0.20 g/L and 2.0 g/L, respectively, under which both the fat separation rate, precipitation rate of milk tea were obviously decreased while its viscosity was increased. Furthermore, the achieved milk tea had a full-body taste and a pleasant odor.

7 Secretory Expression of Monellin in Yeast Pichia pastoris

LIU Xi-chang , ZHANG Yu-jun , YU Hong

2008, 24(1):20-22.

[Abstract](104) [HTML](0) [PDF 1006.66 K](366)

Abstract:
This experiment Pichia codon preference, a high sweetness synthetic gene Monellin, and the reorganization of production of yeast expression vector pPIC9M by electroporation can be a highly efficient expression of secretory activity sweet high sweetness Monellin reorganization Pichia GS115/pPIC9M. Monellin confirmed by PCR gene has been integrated into the yeast genome, SDS-PAGE and Western blot hybridization know immune expressed protein is the target protein final adoption of double-blind confirmed the presence of normal expression of activity.

8 Microwaves Assisted Extraction of Polyphenol from Pomegranate Peel and its Antioxidant and Antimicrobial Activities

SONG Wei-wei , JIAO Shi-rong , ZHOU Jia , YE Chun-yan

2008, 24(1):23-27.

[Abstract](139) [HTML](0) [PDF 6.05 M](392)

Abstract:
The extraction conditions of pomegranate polyphenols were optimized by orthogonal and single factor experiments. The results showed that the optimum volume percent of ethanol in the exaction solution, the ratio of pomegranate peel powder to the solution, the microwave power, extraction time and times were 40%, 1:35, 242 w, 60 s and 3 times, respectively. Under those conditions, the yield of polyphenols from pomegranate peel was 26.52%. Besides, the effects of the extract on hydroxyl radical scavenge and lipid peroxidation of mouse liver suspension were investigated and the values of IC50 and EC50 were shown to be 0.288mg/mL and 0.0079 mg/mL, respectively. It was also found that 1 g/L of the pomegranate polyphenol extracts had the highest inhibition effects on both Escherichia coli and Bacillus subtilis with the inhibition zone diameter and minimum inhibitory concentration of 1.15 cm and 0.5 g/L respectively for Escherichia coli, and 1.25 cm and 2 g /L respectively for Bacillus subtilis.

9 Extraction, Isolation and Analysis of the Polysaccharides from Cordyceps Gunnii (Berk.) BerK.

LIU An-jun , ZHONG Yue-ru , ZHU Zhen-yuan , ZHU Chang-mei , MA Lin , MA Zhi-min

2008, 24(1):28-31.

[Abstract](98) [HTML](0) [PDF 680.45 K](260)

Abstract:
Two crude polysaccharides, CPS-50 and CPS-70, were extracted from the mycelium of Cordyceps gunnii (Berk.) Berk. using 30% ethanol. After remove of lipids (by ethyl acetate) and proteins (using sevage method), and precipitation (with 50% and 70% alcohol), the crude polysaccharides were further purified by DEAE-Sephadex A-25 column. The contents and purities of the obtained polysaccharides, named as CPS-1 and CPS-2, were determined using phenol-sulfuric acid method and UV spectrophotometric method, respectively. Their structures were analyzed by infrared spectrum (IR). In addition, thin layer chromatography analysis showed that the hydrolysis of the pure polysaccharides yielded two kinds of monosaccharides, glucose and/or mannose.

10 Preparation Of Manno-oligosaccharides by Enzymatic Acidolysis

XU Mu-dan , TANG Mu-hong

2008, 24(1):32-34.

[Abstract](167) [HTML](0) [PDF 7.79 M](402)

Abstract:
In this paper, the preparation of manno-oligosaccharides (MOS) was studies by enzymatic acidolysis. The preparation conditions were optimized by orthogonal experiment and the optimal acidolysis time, enzymatic temperature, HCl concentration, acidolysis temperature, enzyme dosage, and substrate concentration were 1.5 h, 55 ℃, 0.07 mol/L, 85 ℃, 6000 U/g, and 80 g/L, respectively. And the order of their influences was acidolysis time>zymohydrolysis temperature>HCl concentration>acidolysis temperature>enzyme dosage>substrate concentration.

11 Purification of Total Flavonoids from HangZhou White Chrysanthemum using Macroporous Absorption Resin

YAN Ke-yu , YU Jing

2008, 24(1):35-38.

[Abstract](144) [HTML](0) [PDF 2.23 M](411)

Abstract:
The total flavonoids of Hangzhou white chrysanthemum was purified by macroporous absorption resin and its content was determined by UV spectrophotometry. The optimal purification conditions by AB-8 macroporous absorption resin were as follows: maximum static adsorption capacity of 23.06 mg/g, resin adsorption rate of 89.22%, absorption time of 1 h. Besides, 4BV of 70% alcohol was used as eluent with an eluent rate of 96.64%. AB-8 resin showed good absorption and purification capability of the total flavonoids from HangZhou white chrysanthemum.

12 Study on the Fermentation Techniques of Downy Rosemyrtle Wine

ZHANG Yao-cai , ZHONG Xi-e , JIN Gui-min

2008, 24(1):39-41.

[Abstract](80) [HTML](0) [PDF 1.56 M](314)

Abstract:
The processing of Downy Rosemyrtle wine was studied in the paper. Results showed that the residual sugar content in immobilized cell-fermented downy rosemyrtle wine was lower than that in free cell-fermented wine. Besides, the wine fermented by immobilized cell had the special color of downy rosemyrtle and high clarity. Moreover, study of the immobilized cell-catalyzed fermentation at different temperatures (20 ℃, 25 ℃ and 30 ℃) showed that the wine fermented at 25 ℃ tasted good and had the special color of downy rosemyrtle.

13 Study on the Extaction of Total Flavones from Pueraria Lobata Ohwi

ZHANG Xi-mei , LI Lin , CHEN Ling , MIAO Xiao-jie

2008, 24(1):42-45.

[Abstract](98) [HTML](0) [PDF 10.13 M](364)

Abstract:
The total flavones were extracted with alcohol as the solvent from Pueraria Lobata Ohwi. The effects of the extraction conditions such as the concentration of ethanol, the extraction time, the ratio of Puerariae to solution of ethanol, extraction temperature and the size of Pueraria Lobata Ohwi were studied. The proper condition was determined by orthogonal experiment. The results were as following: The extraction temperature was 80 ℃, the extraction time was 120 min, the concentration of ethanol was 70%, the ratio of Puerariae to solution of ethanol was 1:12, and the size of Pueraria Lobata Ohwi was 80.

14 Production Technology of Stirred Mushroom Yoghurt

HUANG Cong-liang , JIANG Da-wei

2008, 24(1):46-47.

[Abstract](97) [HTML](0) [PDF 229.76 K](256)

Abstract:
A stirred mushroom yoghurt with high quality was fermented by acetic acid bacteria using mushroom and fresh milk as main materials. The processing conditions were optimized by orthogonal experiments.

15 Study of the Water Extraction of Baicalin

ZHENG Bi-sheng , WANG Neng-qing , ZHAO Xin

2008, 24(1):48-51.

[Abstract](169) [HTML](0) [PDF 8.03 M](482)

Abstract:
The effects of several influential factors on the water extraction of baicalin were investigated by single factor experiment using UV-spectrometry for the determination of baicalin content. The extraction conditions were further optimized by orthogonal design. Results showed that, when the materials were crushed into pieces, the best water-solid ratio, reflux extraction times and the extraction time per times were 8, 3, and 1.5 h, respectively, under which the extraction yield reached 23.0491%.

16 Study on the Hydrolysis Process of Zein

ZHAO Hua , WANG Ying , TIAN Shao-ran , GUO Rui

2008, 24(1):52-54.

[Abstract](78) [HTML](0) [PDF 6.73 M](533)

Abstract:
The hydrolyzate of zein catalyzed by Alkaline was hydrolyzed by papain and complex flavor enzymes. The optimum hydrolysis conditions were determined by single factor and orthogonal experiments and the substrate concentration, temperature, pH, papain dosage, dosage of the complex flavor enzymes and reaction time were 70 g/L, 55 ℃, 6, 5%（E/S）, 4.5%（E/S） and 2 h, respectively. Under those conditions, the content of amino nitrogen reached 2.5654 mg/mL.

17 Processing Technologies for Preparation of Antihypertensive Peptides from Recombinant E.coli BL21

WU Ya-li , DENG Mao-cheng , LIANG Shi-zhong

2008, 24(1):55-58.

[Abstract](124) [HTML](0) [PDF 5.11 M](465)

Abstract:
The preparation of AHP from recombinant E.coli BL21 by affinity chromatography and thrombin mediated cleavage was studied through orthogonal experiment. RP-HPLC was used to detect the antihypertensive peptides in AHP. The optimum loading quantity of sample and flow rate were 4 mg (GST-AHP)/mL (medium bed) and 1.5 mL/min, respectively, under which, the absorption capacity of fusion protein was 3.86 mg/mL (medium bed) with a recovery rate of 96.5%. The optimum cleavage conditions of fusion protein by thrombin were as follows: thrombin dosage of 15 U /mg GST, reaction time of 8 h and temperature of 22 ℃, under which 102 μg of AHP could be obtained using 1 mg GST-AHP. In addition, bioactivity of recombinant AHP was studied. The obtained AHP showed high bioactivity in vitro with IC50 and Km of 4.6 μmol/L and 1.95 mmol/L, respectively.

18 Extraction of Chlorophyll from Filter Mud with Microwave Pretreatment

WANG Xiao-fei , GUO Hai-rong , LI Zhi-chun , MO Bin

2008, 24(1):59-60.

[Abstract](112) [HTML](0) [PDF 4.36 M](385)

Abstract:
Extraction process of chlorophyll from the microwave radiated filter mud was investigated. The best procedure was as follows: after adding 5 mL of 60% alcohol to dissolve 5 g of dried filter mud, the mixture was pretreated by microwave, and then extracted for 30 min by 50 mL of 95% alcohol at 60 ℃. Under the above-mention conditions, the chlorophyll content reached 45 mg/mL, which was higher than that by traditional method (23.86 mg∕mL). And the extraction time was shorter than that of the traditional method (4~12 h).

19 Study of Extraction of Procyanidins from Grape Seeds

LI Rui-li , MA Run-yu

2008, 24(1):61-63.

[Abstract](94) [HTML](0) [PDF 1.27 M](377)

Abstract:
Extraction of Procyanidins from grape was studied with three methods, including ultrasonic wave assisted extraction, microwave-assisted ethanol reflux extraction and ethanol reflux extraction. The results indicated that, among the three methods for procyanidins extraction, microwave-assisted ethanol reflux extraction was the most suitable one. Under the optimized microwave-assisted extraction conditions, the extraction rate and purity of propitious were 1.847% and 43.95%, respectively, which were 22.2% and 14.0% respectively higher than those by ultrasonic wave assisted extraction, and 69.8% and 12.1% respectively higher than those by ethanol reflux extraction.

20 Application of Ultrasonic to Polysaccharide Extraction from Termitomyces albuminosus

SHI Bi-bo , LUO Xiao-miao , LIU Man

2008, 24(1):64-66.

[Abstract](99) [HTML](0) [PDF 6.06 M](489)

Abstract:
The application of ultrasonic to polysaccharide extraction from Termitomyces albuminosus was investigated. And the best ratio of water to Termitomyces albuminosus, ultrasonic power, ultrasonic time, water temperature, extraction time and ethanol concentration were 10 times, 100 W, 10min, 80 ℃, 120 min and 90%, respectively. Under those conditions, the yield of crude polysaccharide reached 19.78%, which was higher than that without ultrasonic pretreatment. The content of purified polysaccharide by Sevage reached 87.23%.

21 Hydrolysis of the Buckwheat Protein by Neutral Protease

TIAN Bin , DONG Wen-bin , ZHU Zhen-Bao

2008, 24(1):67-68.

[Abstract](114) [HTML](0) [PDF 6.47 M](428)

Abstract:
Hydrolysis of the buckwheat protein by neutral protease was studied in this paper. The result showed that the optimum enzyme doses, substrate concentration, temperature, pH and reaction time were 5000 U/g, 4%, 50 ℃, 7.5 and 2 h, respectively. Under those optimized conditions, the hydrolytic degree of buckwheat protein reached 42.31%.

22 Technical Processing of a Jelly using Quail Eggs

JIANG Chang-xing , JIAO Yun-peng

2008, 24(1):69-71.

[Abstract](163) [HTML](0) [PDF 288.94 K](276)

Abstract:
In this paper, a new jelly was produced using quail eggs as raw material and the corresponding sensory evaluation standards were recommended. Results showed that, for the processing of quail egg jelly, the best contents of carrageenan, suger, quail-eggs and citric acid were 0.8%, 15%, 15% and 0.25%, respectively, under which the product had good flavor and nice taste.

23 Detection of Chloramphenicol Residue in Freshwater Fish and Milk by Dot Immunogold Filtration Assay

WANG Wei , ZHANG Yan , LIU Jun-wei , WANG Li , WANG Feng-xia , WANG Shuo

2008, 24(1):72-75.

[Abstract](101) [HTML](0) [PDF 1.94 M](374)

Abstract:
An immunogold assay for detection of chloramphenicol (CAP) residue in freshwater fish and milk was established using the colloidal gold with the average diameter of 20 nm for labeling the anti- chloramphenicol (CAP) polyclonal antibody. CAP was conjugated to (OVA) and then they were dispersed on a nitrocellulose (NC) membrane forming the test zone. Goat anti-rabbit IgG was chose as control zone. The method was simple and fast (10~15 minutes) with the sensitivity of 1 μg/L, which could be widely used to detect the CAP residues in freshwater fish and milk.

24 Determination of Cordycepic Acid Content in the Mycelia of Cordyceps brasiliensis Henn by Colorimetry

CAI You-hua , FAN Wen-xia , LIU Xue-ming , XU Yu-juan , WU Yu-ming

2008, 24(1):76-79.

[Abstract](115) [HTML](0) [PDF 307.53 K](317)

Abstract:
A simple, accurate and rapid colorimetry method for the determination of the cordycepic acid content in fermented mycelia of cordyceps brasiliensis henn was established. The results showed that characteristic absorption peak of cordycepic acid, mannitol, was found at 412 nm. Within a linear range of 10~50 mg/L, the regression equation was: Y=0.0102X-0.0029, R2=0.9989. The precision of the instrument was satisfactory with the RSD of 0.35% (n=6). The average recovery and RSD (n=5) were 100.30% and 1.44%, respectively. Among the tested sugar, D-fructose showed high effect on the test results. No D-fructose was found in fermented mycelia of cordyceps brasiliensis, which proved the colorimetric method was suitable for the determination of cordvcepic acid content in fermented mycelia of cordyceps brasiliensis henn. The mannitol content in fermented mycelia of brasiliensis was found to be 8.45% with the RSD value of 0.34%.

25 Analysis of Fragrant Ingredients of Natural Milk Flavor by GC-MS

YU Tie-mei , WANG Wei-fei , YANG Bo , WANG Yong-hua , ZHANG Shui-hua

2008, 24(1):80-82.

[Abstract](147) [HTML](0) [PDF 7.40 M](443)

Abstract:
The fragrant ingredients of two kinds of natural milk flavors were analyzed by the gas chromatography-mass spectrum (GC-MS). According to the standard mass spectrum gallery, the main fragrant ingredients in the two kinds of flavors were determined as lactones, short and middle chain fatty acids, esters and thiazoles, among which the content of δ-decanolatone shown to be the highest. Under the same analysis conditions, the main fatty acids and the minim matters in the hydrolyzed anhydrous cream were also determined by GC-MS.

26 Quantitative Analysis of Narigin and Hesperidin contents in Citrus Aurantium Juice by Reverse-phase High-performance Liquid Chromatography

HE Jian-xin , ZHANG Qun , LIN Shu-hua

2008, 24(1):83-85.

[Abstract](97) [HTML](0) [PDF 7.65 M](388)

Abstract:
The chromatographic conditions for quantitative analysis of narigin and hesperindin contents in citrus aurantium juice were investigated and the optimal reverse-phase chromatogram conditions were found as follows: methanol/water being of 40/60, column being of ODS-C18 (4.6 mm´150 mm), flow rate of 1mL/min and the detection wavelength being of 285 nm. It was also found that the contents of narigin and hesperidin in citrus aurantium juice via methanol dissolve and supersonic treatment were 32% and 4.4% higher than those via water dissolve and standing storage, respectively. The method was simple and accurate.

27 Rapid Detection of Lactobacillus in Food by Real-time PCR

TONG Rui , ZHANG Yuan , ZHENG Qiu-yue , XIE Ming-jie , CAO Ji-juan

2008, 24(1):86-88.

[Abstract](125) [HTML](0) [PDF 508.87 K](363)

Abstract:
In this article ,we established a rapid method for detection of Lactobacillus in food using real-time PCR technique. General primers and specific probes were designed to detect the 16S rDNA sequence of Lactobacillus by TaqManTM real-time PCR assays using non-homologous strains for specific test. The results indicated this method was rapid, sensitive and with high specificity for the detection of Lactobacillus in food.

28 Determination of Caffeine in Baked Coffee by HPLC

ZHAO Dan-xia

2008, 24(1):89-90.

[Abstract](113) [HTML](0) [PDF 265.74 K](297)

Abstract:
A simple, accurate and reproducible method for the determination of caffeine in baked coffee by HPLC was established. The samples were analyzed by gradient elution on a UltimateTM XB-C18 column (5 µm, 4.6×150 mm) using a UV detector at 286 nm. The mobile phase was a mixture of methanol and deionized water(containing 2ml/L of acetic acid) at a flow rate of 1.0mL/min and the column temperature was 30 ℃. Results showed that the standard curve of caffeine had good linearity in the concentration range of 5~200 µg/L (R2=0.9997). The average recovery rate and RSD were 97.93% and 0.14%, respectively.

29 Research Progress in Extraction of Dietary Fibers from Different Materials and their Characteristics

WU Hui , HOU Ping , LI Xiao-feng , YU Yi-gang , LIU Dong-mei

2008, 24(1):91-95.

[Abstract](86) [HTML](0) [PDF 282.05 K](639)

Abstract:
In this paper, recent research progress in extraction of dietary fibers from several kinds of materials, including gain, bean, fruit, vegetables, etc. was reviewed. And the characteristics and application of these dietary fibers were compared.

30 Physiological Functions and Pharmacological Activities of Betaine

LIU Yan-yan , ZENG Xin-an , ZHU Si-ming , SUN Wei-dong

2008, 24(1):96-100.

[Abstract](189) [HTML](0) [PDF 7.84 M](909)

Abstract:
The physiological functions and pharmacological activities of betaine were reviewed in this paper. Betaine could be used as a methyl donor for methionine metabolism and osmosis-regulator for cell response under hypertonic stress. It also showed pharmacological activities towards the diseases of heart, nerve and liver, and hyperhemicysteinuria. The functions and activities of betaine provided a basis for the application of netaine as vessel additives and human drug candidate.

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Volume 24,Issue 1,2008 Table of Contents