Volume 23,Issue 12,2007 Table of Contents

1 Study on Immune Function of the S180 Tumor-bearing Mice Cured by Cartilage Polysaccharide

LIU An-jun , LIU Shuang , CAO Dong-xu , YUAN Bo , WANG Wei-jun , YAN Yan

2007, 23(12):1-4.

[Abstract](126) [HTML](0) [PDF 11.61 M](368)

Abstract:
Objective: To study the immune function of the S180 tumor-bearing mice cured by cartilage polysaccharide, the effects of virucidal S180 tumor on immune function of the mice and the mechanisms. Methods: The influences of the blood serum, spleen cells and thymucytes of the S180 ascites tumor-bearing mice cured by cartilage polysaccharide on the S180 tumor and MCF-7 breast carcinoma cells were measured using the MTT method. Virucidal S180 tumor cells were injected into healthy mice and their effect on immune functions of the mice were investigated. Results: The blood serum, spleen cells and thymucytes of the S180 tumor-bearing mice cured by cartilage polysaccharide had definite inhibitory effects on tumor growth. When cultured in the RPMI 1640 culture-medium, the inhibition rates of 10% blood serum of the S180 tumor-bearing mice cured by cartilage polysaccharide reached 64.09 %, 73.48 %, respectively, while those of 20% blood serum of the mice were 88.70 % and 96.80 %, respectively. Conclusion: The S180 tumor-bearing mice cured by cartilage polysaccharide had definite immune functions on tumors.

2 Effects of Salinity and Its Regulation Ways on Growth and EPA Accumulation of Nannochloropsis oculata

WU Rui-shan , WEI Dong

2007, 23(12):5-8.

[Abstract](117) [HTML](0) [PDF 1.83 M](398)

Abstract:
The effects of salinity and its three regulation ways on cell growth and EPA accumulation of Nannoxhloropsis oculata are investigated in this paper. It is found that 14.5‰~33.5‰ of salinity has little influence on the EPA content of N. oculata, but greatly affects the cell growth. The best range of the salinity for the cell growth and EPA accumulation of N. oculata was determined to be 27.0‰~29.0‰. Besides, similar effects on the cell growth and EPA accumulation are found with the salinities regulated by sea water and sea salt. However, using NaCl to regulate the salinity can not efficiently improve the cell growth and EPA accumulation.

3 Preliminary Investigation of the Effect of Low-Sodium Intake on Hypertention-related Gene Network

ZHANG Zhi-zhou , WANG Xiu-jin , CHANG Li-jun , HAN Xiao , LIU An-jun

2007, 23(12):9-12.

[Abstract](88) [HTML](0) [PDF 5.48 M](337)

Abstract:
Objective: to investigate the effect of low-sodium intake on the expression of hypertention related genes. Method: Six people were apportioned into a control and a patient group (n=3 each) to take the low-sodium intake for two months. Their total RNA samples were extracted, purified and treated by reverse-transcription. Then the gene expression profiles were analyzed by 22 K Human Genome Array experiments. Result: Among the 150 hypertension candidate genes, differences in some gene expression were found in both of the examined groups (the ratio of >2 and<0.5 were regarded as up-regulated and down-regulated expression, respectively). The expression levels of ADA and NPRB genes were decreased in the patient group, while that of the SLC8A1 gene increased in both the groups. Conclusion: Our preliminary investigations provided evidence that consumption of low-sodium salt could adjust local network status of some hypertension candidate genes functionally linked with iron channels/transporters.

4 Study on the Influence of the External Environment of Vertical Refrigerated Display Cabinet on the Food Temperature

XUE Mu-dan , LV Yan-li , SHI Hai-jun , YUAN Pei , WANG Sheng-ruan

2007, 23(12):13-14.

[Abstract](95) [HTML](0) [PDF 225.98 K](267)

Abstract:
The influences of environmental temperature, humidity and light intensity of vertical open-refrigerated food display cabinet on the food temperature were studied. The results showed that the food temperature gradually rose with the increase of the environmental temperature, humidity and the light intensity. When the light intensity was raised to a certain level, a small extra increase in the light intensity may greatly influence the food temperature. So, lowering the environmental temperature, humidity and lighting intensity could decrease the temperature of the frozen food, maintain their qualities, and save the energy consumption of the food display cabinet.

5 Preparation and Structure Characterization of Oligo-chitosan Cr(Ⅲ) Complex

XU Mu-dan , WANG Xiao-Yan

2007, 23(12):15-18.

[Abstract](86) [HTML](0) [PDF 474.27 K](330)

Abstract:
A chitooligosaccharide Cr (Ⅲ) complex was prepared using water soluble chitooligosaccharide and Cr (Ⅲ) ion as substrates and the effects of four factors, including pH value, temperature, substrate concentration and reaction time, on the forming of the complex were investigated. Structure analysis of the complex with IR and UV-Vis spectrophotometric methods showed that the chitooligosaccharide Cr (Ⅲ) complex had high stability and formed via the -NH2 group. The mass percent of Cr reached 25.04%.

6 Study on the Storage Technique of Xinjiang Seed Potatoes

GE Liang , YANG Qing-xiang , PAN Feng , TONG Jun-mao

2007, 23(12):19-22.

[Abstract](88) [HTML](0) [PDF 4.82 M](327)

Abstract:
In this paper, the storage conditions of Xinjiang seed potatoes were studied and the contents of dry matter, starch, protein, reducing sugar and Vc in seed potatoes during storage were analysized. The results showed that the lowest weight loss rate, rotting rate and burgeon rate were obtained by putting the seed potatoes into bags and storing them at 3~5 ℃. The content of Vc gradually decreased with increasing the storage time, and the protein and dry matter contents showed little changes. The content of starch decreased at the initial storage period, and then slightly increased at the last storage period. The change of reducing sugar content was in accordance with the reversible starch-reducing sugar-starch dynamic balance.

7 Study on Elimination Effect of Garlic Extracts on Nitrite in Meat Products

ZHU Feng-mei , DU Bin , SHEN Li , ZHAO Xiao-hui

2007, 23(12):23-25.

[Abstract](99) [HTML](0) [PDF 229.79 K](299)

Abstract:
The elimination effect of garlic extract on nitrite in meat products was discussed in this paper. The elimination rate of garlic on nitrite was determined by N-(1-naphthyl)-ethylenediamine dihydrochloride spectrophotometric method. The results showed that the optimal temperature and time of water bath, the ratio of solid to liquid, the pH value and the reaction time were 80 ℃, 10 min, 1:2, 4.0 and 20 min, respectively. Under those optimized conditions, the nitrite in meat products could be obviously eliminated.

8 Effects of KI and I2 on Preventing the Damage of SO2 on Red Grape during Storage

SUN Jin-xu

2007, 23(12):26-27.

[Abstract](106) [HTML](0) [PDF 197.68 K](242)

Abstract:
The effects of KI and I2 on preventing the damage of SO2 on the red Grape during storage process were explored. The results showed that spraying KI, I2, or their mixture before picking the grape did not influence the preservative effect of SO2. And I2 could enhance the antiseptic effect of SO2, but weaken its anti-browning effect, while KI ccould enhance its anti-browning effect.

9 Effect of Selenium Enrichment Processing on GSH-Px Activity and GSH Content of Mung Bean

WU Xiao-yong , ZHANG Yan-jie , CHEN Keng-keng , CHEN Qi-juan

2007, 23(12):28-30.

[Abstract](161) [HTML](0) [PDF 4.78 M](477)

Abstract:
Glutathione Peroxidose (GSH-Px) is a selenium-dependent enzyme which had been found in animal cells at first, and then has been discovered in many plants in recent years. Researchers has found that preferential concentrations exogenous Se could increase the GSH-Px activity of organism. In order to investigate the relationship between Se and the GSH-Px activity of mung bean, the mung bean had been soaked in a NaSe2O3 solution for 5 hours, and then been germinated in the ambient for a given period. The Se content and the GSH-Px activity of the processed mung bean were determined. Results indicate that exogenous Se increases the activity of GSH-Px in mung bean. Regardless of the immersing stage or the germinating stage, the GSH-Px activity of the selenium processed mung bean is higher than the same of the counterpart. After soaking in the selenium solution for 5 hours, the GSH-Px activity of the mung bean is remarkably increase, 59.38% higher than the same of the counterpart.

10 Ultrasonic-assisted Extraction of Tectoridin from Rhizoma Belamcandae

DUAN Li-hong , ZHENG Bi-sheng , GUO Si-yuan

2007, 23(12):31-32.

[Abstract](131) [HTML](0) [PDF 2.38 M](351)

Abstract:
Objective: to study the ultrasonic-assisted extraction of the active compound Tectoridin from Rhizoma Belamcandae. Methods: the effects of extraction time and ultrasonic power were investigated by the orthogonal experiment and the Tectoridin content was determined by UV-spectrophotometer. Conclusion: The optimal ultrasonic power, ultrasonic frequency, ratio of liquid (70% ethanol) and solid (Rhizoma Belamcandae) and reflux extraction time were 180 W, 45 kHz, 9:1 and 60 min, respectively.

11 Optimization of the Film-forming Conditions of SPI Film

LUO Li-juan , XIONG Jian

2007, 23(12):33-36.

[Abstract](96) [HTML](0) [PDF 249.09 K](289)

Abstract:
In this paper, soy protein isolate edible films were prepared via a wet process. By L9(34) orthogonal experiments, the effects of the concentrations of SPI and glycerol, pH and temperature on the properties of SPI films were studied. Results showed that the optimal SPI concentration, glycerol concentration, pH value and temperature were 5.0% (w/w), 2.0% (w/w), 10 and 90 ℃, respectively.

12 Primary Study on the Vitamin E of Cordyceps gunnii (Berk.) Berk.

ZHU Zhen-yuan , LIU Nian , ZHANG Wei , CHEN Xi , LIU An-jun

2007, 23(12):37-39.

[Abstract](91) [HTML](0) [PDF 5.39 M](398)

Abstract:
Cordyceps gunnii (Berk.) Berk. is one of the important species of Cordyceps spp, which has similar constituents and pharmacological activities to the traditional Chinese medicine Cordyceps sinensis. In the paper, the extraction and separation of vitamin E from Cordyceps gunnii (Berk.) Berk. were studied using 30%, 50%, 80% ethanol solution and ethyl acetate. The content of the crude vitamin E was analyzed with thin layer chromatography (TLC) and UV spectrophotometer methods. The results showed that the higher the organic solvent content in the extracting solution, the higher the content of vitamin E and the lower the purity of the vitamin E. Besides, ethyl acetate was shown to be the best organic solvent for the extraction of vitamin E from Cordyceps gunnii.

13 Supercritical Carbon Dioxide Extraction of Tea Seed Oil

GUO Hua , LUO Jun-wu , ZHOU Jian-ping

2007, 23(12):40-43.

[Abstract](91) [HTML](0) [PDF 299.75 K](332)

Abstract:
In this paper, the application of supercritical carbon dioxide in the extraction of tea seed oil was introduced and the influences of extracting pressure, extracting temperature, the particle size of materials and extracting time on the yield and quality of the tea seed oil were investigated. The optimal extraction conditions were determined to provide a basis for mass production of the tea seed oil.

14 Extraction and Preliminary Analysis of Glycolipids From Fish Head

LIU An-jun , LIU Juan-juan , CAO Dong-xu , WANG Yue-wei

2007, 23(12):44-46.

[Abstract](88) [HTML](0) [PDF 3.89 M](337)

Abstract:
In this study, glycolipids was extracted from heads of carp cyprinoids with chloroform and methanol. The best extraction conditions were determined by single factor test and as follows: the ratio of solid to liquid of 1:15, extracting temperature of 28 ℃ and stirring time of 24 h. The achieved crude glycolipid was then purified by column chromatography and the best developing solvents were the mixture of chloroform, methanol and water with their ratio being of 100:20:12:5. Besides, iodine gas was chosen as the best colour developing reagent.

15 Optimization of Extraction Conditions of Total Flavonoids from Sweet Carambola

YAN Quan , LIU Wei-bing , ZHU Qiu-yan

2007, 23(12):47-50.

[Abstract](85) [HTML](0) [PDF 6.60 M](388)

Abstract:
In this paper, the methanol extraction conditions of total flavonoids from sweet carambola were optimized by single factor and orthogonal tests. The results showed that the best extraction time, ratio of solid to liquid and temperature were 3 h, 1:80 and 80 ℃, respectively.

16 Optimization of liquid fermentation medium for the Culture of Ganoderma lucidum

SUN Jin-xu , ZHU Hui-xia , WANG Min , WEI Shu-zhen , WANG-qian

2007, 23(12):51-53.

[Abstract](107) [HTML](0) [PDF 1.93 M](408)

Abstract:
The liquid fermentation medium for the culture of Ganoderma lucidum was studied and the best culture conditions determined by L18 (37) test were as follows (g/L): glucose content of 30, corn meal content of 50, soybean meal content of 10, yeast extract content of 1, KH2P04 concentration of 0.7, MgSO4 7H2O concentration of 0.1, VB1 content of 0.02, pH value of 6.0.

17 Study of Decoloring Conditions of Sucrose Solution by Coconut Shell Active Carbon

LI Rui , CHEN Hua , XIA Qiu-yu , ZHAO Song-lin , LI Mei-qiu

2007, 23(12):54-55.

[Abstract](136) [HTML](0) [PDF 5.61 M](378)

Abstract:
The decoloring conditions of sucrose solution by coconut shell active carbon were studied using spectrophotometer at 420 nm. It indicated that the best amount of coconut shell active carbon, the pH value of the solution, temperature and the decoloring time were 1.5%, 3.5, 70 ℃ and 20 min, respectively, under which the decoloring rate reached 81.01%.

18 Screening of High Rhamnolipid-producing Mutants Induced by NTG

HUANG Jie , PU Yue-wu

2007, 23(12):56-58.

[Abstract](111) [HTML](0) [PDF 2.06 M](330)

Abstract:
A new NTG-induced mutant of Pseudomonas aeruginosa sp. MIG1.46 was screened using blue gel FP, oil FP and fermentation methods. The yield of rhamnolipid produced by the mutant reached 21.270 g/L, which was 361.0% higher than that by the original strain.

19 Study on the Fermentation Technology of White Gourd Juice with Lactic Acid Bacteria

DENG Kai-ye , XU Cheng-dian

2007, 23(12):59-62.

[Abstract](106) [HTML](0) [PDF 448.15 K](370)

Abstract:
In the current studies, we produced a white gourd lactic acid bacteria drink using the screened and acclimatized bacteria (Lactobacillius Bulgaricus and Streptoccus Thermophilus). The fermentation conditions were optimized and the most suitable lactose content, inoculum size, fermentation temperature and time were 1%, 5%, 39 ℃ and 24 h, respectively. In addition, 7% of white sugar was added as sweetener. The sterilization temperature and time for the fermentation broth were 90 ℃ and 15 min, respectively.

20 The Status Quo of the Frame of Food Safety Forecasting & Pre-warning Information System Foreign & Domestic

YAN Shao-qing , KANG Jun-sheng , QIN Yu-qing , LI Xue-hua

2007, 23(12):63-66.

[Abstract](99) [HTML](0) [PDF 17.16 M](360)

Abstract:
Food safety information system is important to the guarantee of people’s health. The current status of the food safety forecasting & pre-warning information system at home and abroad was introduced and the suggestions to improve our national food safety forecasting & pre-warning information system were put forward.

21 Research Progress in Degradation of Organophosphorus Pesticide by Ultrasound / Ozone

XIAO Hai-yan , HUANG Si-han , QIU Tai-qiu , FAN Xiao-dan

2007, 23(12):67-70.

[Abstract](100) [HTML](0) [PDF 391.03 K](357)

Abstract:
Many studies result showed that the technologies on degradation of organophosphorus pesticide are captured much attention. The physical and chemical mechanism and research achievenment of degradation of organophosphorus pesticide was rewiewed in this paper. It also discussed the future development of this field.

22 Standardization of Food Lab Management

CHEN Yu

2007, 23(12):71-74.

[Abstract](123) [HTML](0) [PDF 13.65 M](404)

Abstract:
According to the national standards and criterions, and our own work experience, suggestions on the standardization of Food laboratory management were addressed in this paper in order to improve the accuracy and efficiency of the test results and enhance the comparability of detection data in different laboratories．

23 Determination of the Sialic Acid Content in Milk by HPLC

FENG Jun , LI Hong-ji , HAN Li-qiang , LI Wei-hua , LI Ping , YANG Guo-yu

2007, 23(12):75-76.

[Abstract](118) [HTML](0) [PDF 283.83 K](282)

Abstract:
Sialic acid in milk was released via acid hydrolysis, and then derivated with O-pheny-lenediamine •2HCl (10 mg/mL) at 80 ℃ for 40 min. The content of the derivated sialic acid was determinded on a Symmetry C18 column (250 mm×4.6 mm, 5 μm) using a UV detector at 230 nm. The mobile phase was a mixture of 1.0% tetraphdrofuran (containing 0.2% phosphoric acid) and acetonitrile (92:8) at a flow rate of 1.0 ml/min. The column temperatue was 35 ℃. The results indicated the linear rang of Sialic acid was 10~320 μg/mL and the average recovery was 95.7%. This method for the determination of the sialic acid content in milk had good reproducibility and high sensitivity.

24 Analysis of Basic Composition and Amino Acid Composition of Livestock Skin and Fish Scale

GAO Jian-hua , NING Zheng-xiang

2007, 23(12):77-79.

[Abstract](106) [HTML](0) [PDF 163.00 K](273)

Abstract:
In this paper, the basic composition and amino acid composition of some livestock skins and fish scales were studied. The results showed that both of the livestock skin and fish scale were rich in 18 kinds of amino acids and hydroxyproline with similar amino acid composition, and they could be used as a new source of collagen.

25 Glutaraldehyde Residue Determination in Chutty by HPLC

WANG Miao-fei , GUO Xin-dong , DU Zhi-feng , WU Yu-luan , WANG Yong-hua , ZHANG Shui-hua

2007, 23(12):80-82.

[Abstract](166) [HTML](0) [PDF 5.84 M](434)

Abstract:
A method for the determination of glutaraldehyde residue in chutty by HPLC was developed. In the range of glutaraldehyde content of 0.2~5 mg/L, the method was fast and accurate with a linear correlation of 0.9995, average recoveries of 77.2%~83.9%, a relative standard deviation of 4.95%~6.22% and a minimum determination concentration of 0.2 mg/L.

26 Determination of Malathion Residue in Rice by Gas Chromatography

XIAO Qing , CHEN Shu

2007, 23(12):83-84.

[Abstract](97) [HTML](0) [PDF 3.26 M](330)

Abstract:
The determination of malathion residue in rice by capillary column gas chromatography was studied. The linearity is satisfactory with a correlation coefficient of 0.998 at concentrations ranging from 0.01 g/mL to1.00 g/mL. The detection limit and average recovery of malathion were 0.004 mg/kg and 97.2%, respectively. This method was fast, simple and accurate, which could be applied for determination of malathion content in rice.

27 Progress of Lipid Microencapsulation by Spray-drying

YAO Xuan , TAO Ning-ping , WANG Xi-chang

2007, 23(12):85-89.

[Abstract](115) [HTML](0) [PDF 5.39 M](419)

Abstract:
Microencapsulation of lipid has been widely used in food industry recently. In this review the technologies of lipid microencapsulation were briefly introduced, and the process technology, the developments and improvements of spray-drying were also summarized to provide references for further research.

28 Research on the Application of Biotechnology in Modern Cigarette Industry

RAO Guo-hua , SHEN Guang-lin , WEN Dong-qi , ZHAO Mou-ming

2007, 23(12):90-94.

[Abstract](112) [HTML](0) [PDF 312.39 K](279)

Abstract:
Biotechnology has been widely applied in modern tobacco industry, which could not only improve the quality of tobacco material, but also decrease the harm of cigarette to consumers. The applications of biotechnologies in tobacco fermentation, improvement of tobacco quality, treatment of tobacco pipe and slice, tobacco essence production and bio-filters are reviewed in this paper.

29 Reform and Practice of Food Toxicology Teaching

WANG Yan-bo , HAN Jian-zhong

2007, 23(12):95-97.

[Abstract](99) [HTML](0) [PDF 196.22 K](316)

Abstract:
The traditional and single teaching model needs to be improved to adapt the rapid development of food toxicology and the whole design of food quality and safety. We present here the suggestions on the teaching in class, experimental teaching and examination ways in the reform and practice of food toxicology teaching by means of our rich experiences in this field in order to provide an ideal teaching environment and growth platform for the training of the talents with creative abilities and high qualities.

30 Exploration of Practice Teaching of Microbiology: the Design and Discussion of the Integrated Experiments

LI Xin-she , LU Bu-shi , WANG Fang-yin

2007, 23(12):98-100.

[Abstract](88) [HTML](0) [PDF 171.61 K](233)

Abstract:
Microbiology is a subject with high practical application. Deepening the reformation of experimental teaching is the key to the improvement of teaching effect and the creativity of the students and opening the integrated experiments is one of the important ways to realize those teaching aims.

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Volume 23,Issue 12,2007 Table of Contents