Volume 23,Issue 11,2007 Table of Contents

1 Effects and Mechanism of the Inhibition of H22 Hepatocarcinoma Cell Growth by Cartilage Polysaccharide

LIU An-jun , CHEN Yan-yan , ZHANG Guo-rong , CHE Wei , MENG Yan-li

2007, 23(11):1-3.

[Abstract](104) [HTML](0) [PDF 3.40 M](278)

Abstract:
The effect and mechanism of inhibition of H22 Hepatocarcinoma cell growth by cartilage polysaccharides (CA) were studied. Transplanted H22 hepatocarcinoma was used as the model and the mice were randomly divided into 2 group, the control group and CA group. The growth and apoptosis of the tumor cell were investigated by HE Staining, terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL), flow cytometry (FCM) and immunofluorescent staining. Results showed that CA could induce the apoptosis of the H22 cell, inhibit the growth of tumor and prolong the life span time of the examined mice. Besides, increasing the time after taking medicine could enhance the expression of P21 protein in the CA group and inhibit the expression of Cyclin D1. As a new anti-cancer agent, CA had wide application prospects.

2 Effects of Alkali and High Temperature Treatments on the Aflatoxins Degradation and Enzymatic Hydrolysis of Peanut Dregs

GE Yun-xia , ZHAO Mou-ming , CUI Chun , KONG Ling-hui , WU Xiao

2007, 23(11):4-6.

[Abstract](86) [HTML](0) [PDF 252.63 K](230)

Abstract:
The effects of alkali and high temperature treatments on the aflatoxins degradation and enzymatic hydrolysis of peanut dregs are studied in this paper. Results show that the alkali and high temperature treatments contribute to the degradation of aflatoxins in peanut dregs and the enzymatic hydrolysis of peanut dregs. The optimized conditions are as follows: temperature of 121 ℃, pH value of 10 and treatment time of 60 min, under which 84.50% of aflatoxins are decomposed and the concentration of the aflatoxins in the enzymatic hydrolysate is 0.344 ng/ml. Besides, the recovery rate of protein and amino nitrogen content in the hydrolysate are 27.44% and 57.70% respectively higher than those of the control group.

3 Study on the Stability of the Purple Corn Pigment

SHI Hai-ying , LV Xiao-ling

2007, 23(11):7-10.

[Abstract](89) [HTML](0) [PDF 7.65 M](344)

Abstract:
The effects of pH value, temperature, metallic ion, light, food additives, irradiation and high pressure on the stability of purple corn pigment were systematically researched in this paper. Results showed that purple corn pigment was stable under the examined conditions and the highest stability of the pigment was found under acidic condition. This pigment had values for further development in food industry.

4 Study on the Properties of Low Cross-linked Succinylated Potato Starch

ZENG Zhen , WU Ying-long , LU Yang

2007, 23(11):11-14.

[Abstract](88) [HTML](0) [PDF 9.16 M](381)

Abstract:
The succinylated and low cross-linked succinylated potato starch was prepared in water medium and a comparison of its properties with the untreated potato starch, including submicroscopic morphology, RVA profile, transparency, stability of retrogradation, was also made. The results indicated that the transparency, stabilization of retrogradation stability of the potato starch were obviously improved by succinylating and low cross-linked succinylating. Furthermore, the transparency, stabilization of retrogradation and emulsification stability of low cross-linked succinylated starch were higher than those of the succinylated starch.

5 Effect of Vegetable Oils Addition on Arachidonic Acid Production by Mortieralla sp.

LIN Wei-tie , ZHU Min

2007, 23(11):15-18.

[Abstract](86) [HTML](0) [PDF 8.62 M](384)

Abstract:
The Effects of addition of four kinds of vegetable oils (soybean oil, palm oil, peanut oil and sesame oil) on arachidonic acid production by Mortierella sp. were studied. The results indicated that vegetable oils markedly enhanced the mycelial lipid accumulation. When Mortierella sp. was cultured in medium with vegetable oils as single carbon source, arachidonic acid content in lipids of the fungi greatly decreased compared with that using glucose as single carbon source. For that cultured in medium with vegetable oil and glucose as carbon sources, the arachidonic acid content was also lower than that using glucose as single carbon source, but higher than that using vegetable oils as carbon source. These pheromones indicated that vegetable oils inhibited the biosynthesis of arachidonic acid. In addition, the composition of the mycelia lipid was similar to the vegetable oil added in the medium.

6 Immobilization of Trypsin on Mesoporous Silica Material

DONG Ying-chao , QI Tao , QIN Yu-chang , WANG Li-na , LI Jun-guo , ZHANG Yi

2007, 23(11):19-21.

[Abstract](104) [HTML](0) [PDF 1.53 M](309)

Abstract:
The hexagonal silica mesoporous material SBA-15 with ordered 2-dimensional channel structure was used as a support to immobilize the enzyme trypsin which was assembled in the channel of SBA-15 by immersion method. The effects of immobilization conditions such as enzyme concentration, pH value and time on the absorbed amount of enzyme in SBA-15, the thermal and operational stabilities of the immobilized enzyme were studied. And the best enzyme concentration and immobilization time were 5 mg/ml and 10 h, respectively, under which the absorbed amount of enzyme reached 23.6 mg/g. It was also found that the thermal and operational stabilities of the immobilized enzyme were higher than those of the free enzyme. Silica mesoporous material SBA-5 was shown to be a good support for immobilization of enzyme.

7 Evaluation of Antioxidant Effect of Propolis in Edible Oil

ZHU Hong

2007, 23(11):22-23.

[Abstract](110) [HTML](0) [PDF 4.54 M](372)

Abstract:
the antioxidant activity of propolis in lard and rapeseed oil were evaluated by determining their POV values and compared with those of PG. Results showed that propolis could delay the oxidation reactions of lard and rapeseed oil and its antioxidant activity was higher than that of PG at the same concentration. Heating the propolis at high temperature for a short period could not affect its antioxidant activity. Besides, adding citric acid or lecithin could improve its antioxidant activity.

8 Analysis on the Separation and Purification of Immunoglobulin of egg yolk (IgY)

REN Ping-guo , XU Qi-hong

2007, 23(11):24-26.

[Abstract](128) [HTML](0) [PDF 8.49 M](424)

Abstract:
The separation and purification of Immunoglobulin of egg yolk (IgY) were studied. The mixture of yolk and water (1:8, v/v) was stirred for 10 min and then its pH value was adjusted to 5.2 with HCl. After placing the mixture for 2 h at 4 ℃, the supernatant was diluted with 95% ice ethanol (-20 ℃) until its concentration reached 60% (v/v). Then the solution was stirred for 20 min at 4 ℃ and centrifuged (22000 r/min) for 25 min at 4 ℃. The precipitate was collected and added into 0.028 mol/L NaCl solution. The corresponding suspension was filtered at 4 ℃ to remove the lipoprotein sediment and the filtrate was then purified by SDS-PAGE methods, giving the protein IgY with the purify of 98%. Its activity was determined with Escherichia coli (E.coli) and results showed that this purification method could well maintain the activity of IgY.

9 Study on the Release of Kinetic Model of Vitamin A Microcapsule in False Gastric and Intestines Succus

XIE Yan-li , SHI Xiu-li

2007, 23(11):27-29.

[Abstract](159) [HTML](0) [PDF 7.65 M](424)

Abstract:
The release of kinetic model of microencapsulated vitamin A was studied by the first order reaction kinetics in false gastric and intestines juice. The release equation was Y=0.5199e0.0355x and R2 was 0.983 in false gastric juice. The release equation was Y=0.3664e0.0763x and R2 was 0.9829 in false intestines juice.

10 A Study on the Drying Technology of Pineapple Peel

YE Sheng-quan , WU Hui , GUO Si-yuan

2007, 23(11):30-33.

[Abstract](143) [HTML](0) [PDF 517.91 K](422)

Abstract:
The drying technologies of pineapple peel were investigated by varying the drying temperature and material thickness. The analysis of the drying curve showed that the material thickness had higher effect on the drying efficiency than the other examined factor and a positive relevance was found between the drying temperature and drying rate. It was also found that the nutrition component of the dried pineapple peel was influenced by the drying temperature. The optimum material thickness and drying temperature were determined as 6 kg/m2 and 70 ℃, respectively, under which the product was rich in pineapple aroma, loose-textured and with a uniform color.

11 Process Technology for the production of Xylose via Hydrolysis of Straw in a mixture of Formic acid and Hydrochloric Acid

WU Zhen , LIN Lu , DENG Hai-bo , SUN Yong , PANG Chun-sheng , LI Jia-zhe

2007, 23(11):34-36.

[Abstract](115) [HTML](0) [PDF 3.57 M](276)

Abstract:
The production of xylose via hydrolysis of straw in a mixture of formic acid and hydrochloric acid was investigated. Results showed that the yield of straw xylose reached 23.62% with saturated formic acid containing 10% HCl, reaction time of 0.5 h and temperature of 65 ℃. The hydrolysate was purified with D311 and activated carbon for decoloration and then crystallized, giving the pure xylose with the yield of 15.87%. Results indicated that this technology was available.

12 Magnetic Affinity Support for Trypsin Separation and Purification

AN Xiao-ning , GAO Wei

2007, 23(11):37-40.

[Abstract](103) [HTML](0) [PDF 359.69 K](238)

Abstract:
Ultrafine high magnetic affinity support was prepared by coating nanometer barium ferrite with chitosan, cross-linking with epichlorohydrin, and then activating with carbodiimide. The analysis result of the dynamic laser scatters indicated that the average diameter of the magnetic particles was 401 nm. The magnetic measurement showed that the ultrafine magnetic particles were high magnetic. At first the aprotinin was considered to be affinity ligand and covalently bound onto the magnetic particles via carbodiimide activation, and then these particles were used for the affinity purification of trypsin. The proposed method was successfully applied to the affinity purification of trypsin with the purified conditions of trypsin affinity under discussing.

13 Persimmon Wine Processes and Craft Research

ZHAO Wen-hong , BAI Wei-dong , YAN Li-yi

2007, 23(11):41-43.

[Abstract](141) [HTML](0) [PDF 270.27 K](366)

Abstract:
The fermentations of persimmon wine were studied through individual factor experiment and orthogonal experiment. Results showed that the wine taste mellow-astringent and have light yellow or golden and smell like fresh persimmon′s fruity after the persimmon slurry fermented 5 days at 28 ℃. Before fermented, the pH value of persimmon slurry were adjusted to 4.0 after enzymatic actions, the sugar degree were 22 °Bx, and added 10% (m/v) Grape wine yeast to the persimmon slurry.

14 Preparation of Raspberry Drink

SUN Jin-xu , WEI Shu-zhen , ZHU Hui-xia , WANG Min , WANG Qing

2007, 23(11):44-46.

[Abstract](134) [HTML](0) [PDF 258.50 K](328)

Abstract:
The preparation of raspberry drink was studied. For the extraction of raspberry, the best conditions was determined by L16 (44) orthogonal experiments and as follows: extraction time of 10 h, extraction temperature of 100 ℃, pectinase dosage of 3200 U/mL (added in the former handling period) and the pH value of 4. Through L9 (34) orthogonal experiments, the optimum formula of the raspberry drink was determined as follows: raspberry extract amount of 35%, white sugar concentration of 25 g/L and citric acid concentration of 3.5 g/L. The ginger-raspberry drink was yellowish, clear and transparent with a mild bitterness, acid taste and the special aroma of raspberry aroma.

15 A Study on Enzymatic Extraction of Capsaicin by Cellulose

LUO Cang-xue , ZHU Niu , LEI Xue-feng

2007, 23(11):47-50.

[Abstract](98) [HTML](0) [PDF 7.47 M](398)

Abstract:
The enzymatic method for the extraction of capsaicin by cellulose (16568 U/g) was developed. The results showed that the best enzymatic hydrolysis conditions for extracting capsaicin were as follows: pH value of enzyme solution of 5.2, the enzyme dosage of 7 mg/g, the reaction time of 3 h, and the reaction temperature of 40 ℃. Compared with the traditional extraction methods, the extraction yield of capsaicin with the new method increased by 16%. Besides, this extraction method was simple, fast and with relative low temperature.

16 Preparation of Soybean Oligopeptides by Alcalase-catalyzed Hydrolysis of Soybean Protein

BAN Yu-feng , ZHU Hai-feng , SHUN Ming-zhu

2007, 23(11):51-53.

[Abstract](77) [HTML](0) [PDF 229.72 K](249)

Abstract:
The effects of several factors on the alcalase-catalyzed hydrolysis of soybean protein, such as the enzyme dosage, temperature, pH value and reaction time, were studied. The orthogonal test showed that the optimized hydrolysis conditions were as follow: pH value of 7.5, reaction temperature of 70 ℃, alcalase dosage of 20 μL/g protein, substrate concentration of 60 g/L, and total reaction time of 4 h. Under these conditions the hydrolysis degree of soybean protein reached 24.1%.

17 Process Optimization for the Preparation of Anticoagulant Cellulose Sulfate

QIU Chang-ling , WANG Zhao-mei , GUO Si-yuan , FENG Min-deng

2007, 23(11):54-57.

[Abstract](71) [HTML](0) [PDF 279.80 K](274)

Abstract:
The preparation of anticoagulant cellulose sulfate was investigated by five factors quadri-rotation orthogonal experiments and statistical optimization analysis. The results showed that the optimum preparation temperature of esterifying agent, esterifying agent dosage, reaction temperature, reaction time and pH value were 20.0 ℃, 6.8 mol/mol AGU, 12 ℃, 3.5 h and 8.0, respectively, under which, the cellulose sulfate had the highest effect on the prolongation of the RT, APTT and TT, meaning that the best anticoagulant activity of cellulose sulfate was achieved.

18 Influence of Xanthan Gum Compounded with Several Colloids on the Stability of Peanut Milk

ZHAO Rong-zhong , ZHOU Xue-song , ZENG Jian-xin

2007, 23(11):58-60.

[Abstract](110) [HTML](0) [PDF 530.12 K](352)

Abstract:
Effects of the mixtures of xanthan gum with locust bean gum, guar gum or konjac gum on the stability of peanut milk were studied. The rate of sample precipitation, rate of fat precipitation, viscosity and thermal stability of the sample were also analyzed. The results showed that the highest stability of peanut milk was obtained with the xanthan gum and guar gum concentrations being of 0.05% and the ratio of oxanthan gum to guar gum being of 1:2.

19 Studies on Selection and Enumberation of Favorable Lactococcus

WANG Zhe , MENG Xian-zhi , ZHAO Wei

2007, 23(11):61-63.

[Abstract](77) [HTML](0) [PDF 1.71 M](258)

Abstract:
The selection and enumberation of Lactococcus Q26 was studied. The results showed that the sucrose, N-sources, the ratio of Peptone and Beef extract and Yeast extract, Yam extract, CaCO3, Vc, and Tomato extract of culture medium is 0.5%, 2.5%, 2:2:1(m/m/m), 5%, 0.3%, 0.3% and 2.5%, respectively. The highest number of Lactococcus can reach 2.36×109 /mL.

20 Preparation of Dietary Fiber from Wheat Straw

JIANG Tu

2007, 23(11):64-67.

[Abstract](89) [HTML](0) [PDF 269.43 K](240)

Abstract:
The preparation of dietary fiber from wheat straw residual was studied by two extraction methods, NaOH and C2H5OH extraction. Single factor and orthogonal experiments were used to determine the best conditions. Results showed that, for the NaOH extraction of the dietary fiber, the optimum temperature, extraction time, concentration of the extracting reagent and amount of the liquid were room temperature, 60 min, 3% and 60 ml, respectively, while for the C2H5OH extraction, the best values of the above-mentioned factors were room temperature, 80 min, 100% and 70 ml, respectively. The extraction technologies were evaluated by analysis of the fiber contents in the extracts by neutral detergent and the determination of the properties of the extracted dietary fiber.

21 Comparison of Hydrolysis of Chinese pine pollen Protein Catalyzed by two Proteases

YIN Shu-hua , GUO Li-mei

2007, 23(11):68-70.

[Abstract](91) [HTML](0) [PDF 173.03 K](277)

Abstract:
The hydrolysis of the Chinese pine pollen protein by alkaline protease and papain was studied and the nitrogen solubility index, hydroxygen free radical-cleaning property and the antioxidant activity of the peptides achieved with two methods were compared. The peptide achieved by alkaline protease-catalyzed hydrolysis had higher nitrogen solubility index and lower hydroxygen free radical-cleaning property and antioxidant activity than that achieved by papain-catalyzed hydrolysis of Chinese pine pollen protein.

22 Rapid Detection of toxic Alexandrium by Loop-Mediated Isothermal Amplification Method

LIU Fang , WANG Li , LI Lin , Shinji Yamasaki , SHI Lei

2007, 23(11):71-74.

[Abstract](129) [HTML](0) [PDF 10.52 M](361)

Abstract:
Loop-mediated isothermal amplification method (LAMP) is a novel nucleic acid amplification technology. The LAMP method amplifies DNA with rapidity, high specificity and sensitivity under isothermal conditions. This thesis took toxic Alexandrium as the major research object. DNA template was extracted by boiling method and specific primer was designed for LAMP assays. Toxic Alexandrium were quickly detected by LAMP technology. LAMP and PCR were successfully compared in the sensitivity of detecting Alexandrium minutum. Without gel electrophoresis, the LAMP amplification was directly visualized in the reaction tube by addition of SYBR Green I for a naked-eye inspection. The result indicated that toxic Alexandrium was detected by LAMP technology within 1 h under isothermal condition at 65 ℃. The lower detection limit of the LAMP reaction was 200 cell/ml, while that of the PCR was 1000 cell/ml. The LAMP assay is more simple, rapid and sensitive than the PCR. Furthermore, as LAMP technology does not require sophisticated instrumentation, it is very suitable for diagnosis in the fields.

23 Determination of Formaldehyde in the Production of Beancurd

LIU Zhi-ming , MENG Ling , ZHANG Rui

2007, 23(11):75-77.

[Abstract](111) [HTML](0) [PDF 241.89 K](297)

Abstract:
Formaldehyde was used in the production process of the beancurd using soybean and gluconic acid-δ lactone as main materials. The formaldehyde contents in tofu filtrate, liquid separated from beancurd and the distillate of beancurd were measured by acid-base titration. Under the same experimental conditions and in the tofu samples with same weight, formaldehyde contents in the distillate, filtrate and liquid separated from beancurd were reduced in turn. The recovery rate of formaldehyde with this method was higher than 90 % (not including the beancurd residue). This research provided a simple and accurate method for the determination of formaldehyde in the production of beancurd.

24 Determination of Active Component in Commercial Extracts of Echinacea purpurea by HPLC-PDA

BI Xiao-ling , CAI Miao-yan , WANG Zhao-mei , DENG Yun , ZHANG Gan-yuan

2007, 23(11):78-79.

[Abstract](105) [HTML](0) [PDF 5.91 M](359)

Abstract:
Objective To establish a method for the determination of cichoric acid, an active component in commercial extracts of Echinacea purpurea, by HPLC-PDA. and provide reference for the further use of Echinacea purpurea extracts. Method The samples were analyzed by HPLC-PDA on a C18 column with a PDA at 330 nm and the temperature at 35 ℃. The mobile phase was a mixture of acetonitrile and 0.1% phosphoric acid at a flow rate of 1.5 mL/min. Results The standard curve was linear in the cichoric acid concentration range of 0.456~2.28 μg (r=0.9996). The average recovery was 98.18% (RSD=1.12%). Conclusion The method was rapid, sensitive and had good reproducibility, which could be used for quality assessment of cichoric acid in commercial extracts of Echinacea purpurea.

25 Determination of Chlorogenic Acid in Sunflower Kernel by Thin Layer Chromatography-spectrophotometry

LIU Yan-fang , MA Ge-li

2007, 23(11):80-81.

[Abstract](85) [HTML](0) [PDF 1.13 M](328)

Abstract:
A new method for determination of chlorogenic acid in sunflower kernel by thin layer chromatography-spectrophotometry was established. The chlorogenic acid in the sample was separated by thin layer chromatography. Analytical conditions of the chlorogenic acid were optimized and the effects of some key factors on the determination were discussed. Within the range of chlorogenic acid concentration of 0.20~1 μg/mL, the linear regression equation was A=0.0451c+0.897, with the linear correlation coefficient of 0.9995.

26 The need and referenced text of establishment on a company standard of fruit-flavored beverages

LI Hong , YUAN Ya-juan , ZHANG Ming-quan , LI Hong-liang

2007, 23(11):82-84.

[Abstract](120) [HTML](0) [PDF 255.23 K](279)

Abstract:
The classifications of soft drinks and its units of applying production license in Quality Safety Certification of the products were introduced in this paper, and the related national and industrial standards for soft drinks that have been established were listed. A company standard referenced text based-on these standards for fruit-flavoured beverage was proposed. We also hope that this referenced text will promote the developments of the fruit-flavored beverages companies.

27 Analysis of Food Traceability System in the Food Chain

QIN Yu-qing , GENG Qian-qiang , YAN Shao-qing

2007, 23(11):85-88.

[Abstract](146) [HTML](0) [PDF 4.70 M](362)

Abstract:
By analysis of the definitions of the food chain and food traceability and the requirements of standards of international food traceability, the food traceability system in food chain was introduced. Some proposals were also put forward for the development of Chinese food traceability system.

28 Research Progress of Polysaccharides in Morus alba L. Leaves

YING Zhi , LI Jian-rong , HAN Xiao-xiang

2007, 23(11):89-93.

[Abstract](155) [HTML](0) [PDF 312.40 K](598)

Abstract:
The polysaccharides in leaves of Morus alba L., a kind of the main functional components of the Morus alba L. leaves, possesses various pharmacological and physiological activities and studies on this polysaccharides has become to a research hotspot in recent years. The research progress of extraction, isolation and purification, chemical constitution and bioactivities of the polysaccharides in mulberry leaves were reviewed in this paper, which is helpful to the further research and development of Morus alba L.

29 Research Advance of Magnetic Microspheres and Its Application to Enzyme Immobilization

LI Li , MA Li , LI He

2007, 23(11):94-98.

[Abstract](132) [HTML](0) [PDF 261.29 K](287)

Abstract:
Magnetic polymer microspheres are a novel functional material that has been developed resently.As the carrier of enzyme,cell and drug,it can be widely applied in many fields,such as bio-engineering, cytology, biomedical science and so on. The recent research progesses of magnetic polymer microspheres are reviewed. The preparation and characterization of magnetic microspheres are also introduced. Immobolization of enzyme onto magnetic polymer microspheres is emphasized and every method of immobilization of enzyme is compared and analyzed.The prospects of magnetic polymer microsphere are also proposed.

30 Study on the Establishment of Curricular System of Food Quality and Safety Specialty

XU Xi-lin , WU Hui , SHI Ying , LI Xiao-feng , LIU Dong-mei , YU Yi-gang , YANG Ru-de

2007, 23(11):99-100.

[Abstract](96) [HTML](0) [PDF 132.56 K](256)

Abstract:
Food quality and safety specialty plays an important role in assuring the safety of food chain. However, as a new-built specialty, there are some problems on the establishment of its curricular system. The guideline in the establishment of its curricular system, the selection of teaching materials, and so on, were discussed and some proposes were put forward.

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Volume 23,Issue 11,2007 Table of Contents