Abstract:Rapid quantification of vomitoxin in the edible part of wheat is crucial for improving the acquisition efficiency. To evaluate the application effect of DONQ-FAST5 rapid quantification method for detecting vomitoxin in wheat and its products based on the principle of colloidal gold immunochromatography, quality control samples at three levels of 400, 800, and 1 845 μg/kg were selected and measured in triplicate. Compared with high-performance liquid chromatography (HPLC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS), the recoveries of the three quality control samples determined by DONQ-FAST5 were over 91.00%, which met the requirements of GB/T 27404-2008 "Laboratory Quality Control Specification Food Physical and Chemical Testing". Only the relative standard deviation (RSD) of the standard value of 400 μg/kg was 7.87%, with the RSDs of the remaining samples being lower than 5.00%, indicating good test precision. Under the same conditions, the effects of sample matrix, dilution factor and temperature on the DONQ-FAST5 rapid quantification method were analysed by comparing the results with those of HPLC and LC-MS/MS. The results showed that when the dilution factor was 5 times and the experimental temperature was 25~30 ℃, the DONQ-FAST5 rapid quantitative method was satisfactory in the detection of DON content in whole wheat flour, bran flour and endosperm flour, compared with the instrumental analysis method. Three methods were used to determine the DON content in 371 wheat samples, and the results were consistent. The DONQ-FAST5 rapid quantification method could simultaneously incubate 4 samples per batch, and could accurately quantify the DON content of 4 samples within 15 minutes, providing a theoretical basis for on-site real-time monitoring of wheat vomiting toxin content in wheat by procurement enterprises.
