Abstract:To explore the effects of the treatment with Amomum tsao-ko extract combined with cold atmospheric plasma on the quality changes of Penaeus vannamei during cold storage and preservation, Penaeus vannamei was taken as the research object in this study. Penaeus vannamei was treated by one of the following three methods: Amomum tsao-ko extract (ATK), cold atmospheric plasma (CAP), and the combination of both (ATK+CAP). The total plate count (TPC), total volatile base nitrogen (TVB-N), water-holding capacity (WHC), L* value, a* value, pH, 2-thiobarbituric acid reactive substance (TBARS), sensory score and other indicators were measured to evaluate the changes in quality of Penaeus vannamei during cold storage and the effects of the three preservation methods. The research revealed that all three treatments could effectively retard the deterioration rate of Penaeus vannamei, with the ATK+CAP treatment showing the greatest effect. Using TVB-N as a key indicator, the shelf life was extended by 4 days compared to the control group, with the TVB-N value of the treated sample was 35.19 mg/100 g after 10 days of storage, which was significantly lower than that of the control group (54.04 mg/100 g), the ATK group (47.88 mg/100 g) and the CAP group (49.00 mg/100 g). In the single-treatment groups, the TVB-N value of the single treatment group exceeded 30 mg/100 g on the eighth day, and the shelf life was extended by about 2 days. Meanwhile, in the combined treatment group, their water-holding capacity, color retention and total plate counts were improved compared with the control group (P<0.05). In summary, treating Penaeus vannamei with the Amomum tsao-ko extract combined with cold atmospheric plasma can improve the quality of shrimp during storage and extend their shelf life to some extent, providing theoretical support for research on Penaeus vannamei preservation methods.