Synergistic Effect of D-ribose and Fructose 1,6-diphosphate on Improving Fatigue in Mice

Abstract:

The synergistic effect of the combined use of D-ribose and fructose 1,6-diphosphate (FDP) on anti-fatigue and antioxidant capacity was investigated. Mice were administered the corresponding test substances via gavage for 30 days. Exhaustive swimming experiments were performed, and related anti-fatigue and antioxidant indicators were measured. The exhaustion swimming times of the low-dose D-ribose+medium-dose FDP (DL+FM) group increased by 175.08 and 293.07 s in the last two exhaustive swimming sessions compared to those in the control group. In addition, biochemical experiments revealed a significantly decreased blood lactic acid level and cardiac malondialdehyde content, as well as a significantly increased cardiac catalase content and liver glutathione level (P<0.05) in the DL+FM group. Meanwhile, the liver glycogen content in this group showed the largest increase (13.241 mg/g). Additionally, the blood urea nitrogen level of the medium-dose D-ribose+medium-dose FDP (DM+FM) group decreased significantly by 30.7% (P<0.01). The results demonstrated that the combined use of D-ribose and fructose 1,6-diphosphate displays a synergistic effect at a specific dose, which prolongs the anti-fatigue duration of mice and enhances its efficacy. This could indicate the significance of the coexistence of the primary energy metabolic pathway (tricarboxylic acid cycle) and the bypass metabolism under hypoxic conditions, thus providing theoretical support for the combined use of D-ribose and FDP.