Abstract:Fat-soluble vitamins are essential micronutrients for human growth and development, with deficiencies in these vitamins posing a serious threat to human health. Furthermore, the majority of fat-soluble vitamins cannot be synthesized in the human body and, therefore, must be obtained from external sources. Therefore, rapid and sensitive detection of fat-soluble vitamins is crucial to ensure safe vitamin intake. Although traditional methods such as high-performance liquid chromatography, high performance liquid chromatography, tandem mass spectrometry, and supercritical fluid chromatography offer advantages in terms of selectivity and sensitivity, they also present disadvantages, including complex sample pre-treatment, time-consuming procedures, and dependence on large-scale instrumentation. These constraints have limited the applicability of traditional methods in the rapid detection of fat-soluble vitamins. Recently, biosensors have been used for the detection of fat-soluble vitamins owing to their advantages of high specificity, high sensitivity, low cost, suitability for on-site analysis, and ease of operation. This review initially outlines the physiological functions of common fat-soluble vitamins. Subsequently, it analyzes the advantages and disadvantages of traditional methods and biosensors in detecting fat-soluble vitamins. Finally, it provides a comprehensive summary of the latest research progress on biosensors in the detection of fat-soluble vitamins. This paper outlines the biological components, linear detection range, sensitivity, and detection limits of lipid-soluble vitamin detection biosensors, providing a foundation for the development of future lipid-soluble vitamin detection biosensors.
