Extraction and Analysis of Abalone Visceral Phospholipids and Their Auxiliary Hypolipidemic Effect on High-fat Diet Fed Mice

Abstract:

In this study, Abalone Visceral Phospholipids (AVP) was used as the research object to study its hypolipidemic effect on high-fat diet mice. The components, fatty acid composition and physicochemical properties of AVP were analyzed. The results showed that AVP was composed of phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol, with their contents being 80.69%, 3.66% and 5.06%, respectively. There were five saturated fatty acids and nine unsaturated fatty acids (content: 15.97%). AVP was brownish-yellow particles with a phospholipid smell, acetoneinsoluble matter cntent as 88.3%, content of water and volatiles as 0.8%, n-hexane insoluble matter content as 0.1%, acid value as 34.4 mg KOH/g, and peroxide value as 9.45 mmol/kg, all of which met the national standards. Animal experiments were carried out to verify the auxiliary hypolipidemic effect of AVP on mice fed a high-fat diet. The results showed that compared with the high-fat diet group, the serum TG, TC, and LDL levels in the high-dose AVP group decreased by 47.63%, 32.02%, and 44.73%, respectively, the serum HDL increased by 30.78%, and the AI index decreased by 64.85%. In summary, AVP plays a certain role in assisting the reduction of hyperlipidemia, which provides a theoretical basis for the subsequent development and utilization of abalone viscera resources.