Abstract:Small-molecule hazards are important risk factors in food safety and pose serious threats to human health. Comprehensive screening of food safety risk factors helps enhance the public’s sense of security. However, conventional detection methods fail to meet the requirements for trace detection of small-molecule hazards. The combination of immune response and nucleic acid amplification technology is a rapid, efficient, sensitive, and accurate emerging method for the detection of small-molecule hazards. Immuno-nucleic acid amplification combines the high specificity of immunoassays and the high sensitivity of nucleic acid amplification. This effective, sensitive, and accurate method is gradually playing a more important role in the routine screening of small-molecule hazards. This study reviews the recent advances in research on immuno-nucleic acid amplification, discusses in detail the current state of development of immuno-nucleic acid amplification applicable to non-isothermal and isothermal conditions, and predicts the application prospects of this method in detecting small-molecule hazards in food. Overall, this study provides a reference for further research and promotes the use of this method in food safety inspection.
