Abstract:In this study, an aptamer-based lateral flow test strip was developed for the rapid detection of glyphosate (GLY) using gold nanoparticles labeled aptamers as probes. The effects of experimental conditions, including the concentrations of aptamer and NaCl used, the molar ratio of streptavidin and biotinylated DNA, and the loading quantity of gold nanoparticleaptamer complexes (AuNPs-Apt) on the sensitivity were analyzed. The optimal experimental conditions were as follows: NaCl concentration, 80 mmol/L; aptamer concentration, 500 nmol/L; the molar ratio of streptavidin to Bio-DNAT, 1:3; the loading amount of AuNPs-Apt, 30 μL. Under the optimal experimental conditions, the visual resolution concentration was 20 ng/mL, with a linear range of 5~200 ng/mL and a limit of detection as 5 ng/mL. This aptamer-based strip was applied to the determination of GLY in spiked tea samples, and the recoveries were from 95.13% to 105.24% with relative standards deviation (RSD) of 4.73%~8.28%. All tests could be completed within 5 min. The results showed that the developed aptamerbased lateral flow test strip has the potential to be an alternative tool for the rapid and sensitive detection of GLY.
