Protective Effect of Polysaccharides from Eucommia ulmoides Oliv. Leaves on Mice with Chronic Alcoholic Liver Injury
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Abstract:
To investigate the protective effects of Eucommia ulmoides Oliv. leaves polysaccharides (EULP) against chronic alcoholic liver injury in mice, a chronic alcohol intoxication mouse model was established by intragastric administration of 56% Beijing Hongxing Erguotou for 9 weeks, with EULP intervention administered via gavage for the final 3 weeks of modeling. Hematoxylin-eosin (HE) staining was used to observe the morphological changes in liver and ileum tissues, and the content of interleukin-1β (IL-1β) in liver tissues was determined. Fecal samples from mice were collected for gut microbiota sequencing, and serum non-targeted metabolomics was performed using liquid chromatography-mass spectrometry (LC-MS) technology. In vitro results showed that when the mass concentration of EULP was 1.6 mg·mL-1, the scavenging rates for DPPH, ·OH, and ABTS·+ were 64.51%, 80.15%, and 93.92%, respectively. In animal experiments, compared with the model group, the expression levels of IL-1β in the liver of the medium- and high-dose EULP groups were significantly reduced by 34.87% and 39.93%, respectively, and the degree of injury in mouse liver and intestinal tissues was effectively improved. The abundances of Proteobacteria and Deferribacteres following medium-dose EULP intervention were increased by approximately 112.48% and 175.07% compared with those in the model group. Five potential serum biomarkers were identified through metabolomics analysis, which revealed that liver injury was alleviated by EULP via regulation of the arachidonic acid metabolism pathway. Collectively, EULP was demonstrated to regulate liver injury, inflammatory response, gut microbiota, and serum metabolism, exhibiting potential for treating chronic alcoholic liver injury and providing a theoretical basis for the development of food-derived polysaccharide-based products for alcohol detoxification.
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The National Key Technologies R&D Program of China,
