Antioxidant Active Components of Ethyl Acetate Fractions from the Stems and Leaves of Astragalus membranaceus Based on Spectrum-effect Correlation
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Abstract:
Spectrum-effect correlation was used to screen antioxidant active components from the stems and leaves of Astragalus membranaceus. A total of 19 batches of ethyl acetate extracts from the stems and leaves of A. membranaceus with different geographical origins were used as test samples. High-performance liquid chromatography fingerprints of the samples were established using the similarity evaluation system of chromatographic fingerprints of traditional Chinese medicine, and the in vitro antioxidant activities of different samples were investigated. The correlation between common peaks of the stems and leaves of A. membranaceus and their antioxidant activity indices was clarified using Pearson correlation analysis, partial least squares regression, and grey correlation analysis. The results showed that 13 common peaks (F1~F13) were calibrated from the fingerprints of the 19 batches of samples. The similarity values between samples from each batch and the control fingerprint were 0.912~0.997. Ethyl acetate fractions from the stems and leaves of A. membranaceus with different geographical origins had significantly different antioxidant capacities (P<0.05). Further spectrum-effect correlation analysis showed that F3, F5, F7, F8, F10, and F12 were the main characteristic peaks reflecting the antioxidant activity of the stems and leaves of A. membranaceus. Among them, F5, F10, and F12 were respectively identified as quercitrin, calycosin, and kaempferol through comparison with the reference. This study provides an important theoretical foundation for further development of antioxidant products from the stems and leaves of A. membranaceus.
