Establishment and Application of Multiple qPCR Detection System for Foodborne Pathogens in Aquatic Prefabricated Dishes
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Abstract:
To efficiently detect foodborne pathogens in aquatic prefabricated dishes, a multiplex real-time quantitative PCR (qPCR) system targeting Vibrio parahaemolyticus, Listeria monocytogenes, and Salmonella spp. was established. The performance of this kit, including specificity, sensitivity, anti-interference capability, and stability, was comprehensively evaluated using spiked and real samples. In pure culture, the lowest detection limits of V. parahaemolyticus, L. monocytogenes and Salmonella spp. were 8.67×101 CFU/mL, 3.47×102CFU/mL and 6.56×103 CFU/mL. In spiked samples, after 12 hours of enrichment, minimum detection limits were determined as 6.30×100 CFU/mL (V. parahaemolyticus), 6.13×102 CFU/mL (L. monocytogenes) and 2.44×101 CFU/mL(Salmonella spp.), respectively. Results from 60 real samples were found to align seamlessly with national microbiological standards, confirming high accuracy. Characterized by robust specificity, stability, and interference resistance, this system enables rapid and precise pathogen detection, providing an effective tool for food safety monitoring with substantial application potential.
