Inhibition of UVB-induced Inflammatory Response and Regulation of Antimicrobial Peptide LL-37 by Polysaccharide Extracts from Saussurea laniceps
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
In order to study the effects of Saussurea laniceps polysaccharides (SLPs) on the expression of inflammatory factors and antimicrobial peptide LL-37 in UVB-induced keratinocytes, SLPs were extracted by the ethanol thermal reflux method, and SLPs at different concentrations were used to examine the inhibitory effect of COX-2 (a key mediator of inflammatory pathway). A cell model of UVB irradiation-induced inflammation was established to determine the influence of SLPs on prostaglandin E2 (PGE-2), TNF-α and IL-1β inflammatory factors, as well as the relationships of SLPs with LL-37 expression. An enzyme-linked immunosorbent assay (ELISA) and western blot analysis were used to detect the production of inflammatory factors and LL-37 antimicrobial peptide. The results showed that the inhibition rate of COX-2 was 82.41% at 1 000 μg/mL, and the expression of PGE-2, TNF-α and IL-1β inflammatory factors in HaCaT cells was significantly downregulated at 100 μg/mL (P<0.01). In addition, SLPs at 50 μg/mL and 100 μg/mL concentrations enhanced the expression of LL-37 antimicrobial peptide (P<0.01), thereby down-regulating the expression of TNF-α and IL-1β inflammatory factors, then reducing skin inflammation. Conclusion: SLP can significantly inhibit the inflammatory response induced by UVB, and can further slow down the damage caused by inflammation to the skin by regulating LL-37 antimicrobial peptides, which has the potential to prevent skin inflammatory damage caused by UVB irradiation.
