Comparative Study on In Vitro Effects of Different Polysaccharides Enriching Gut Prevotella Copri
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Abstract:
Natural polysaccharides could regulate gut microbiota to promote host health and have gained significant attention in recent years. Prevotella copri, a common Prevotella species in the gut microbiota, is closely related to human health. To investigate the differential enrichment effects of xylan, xyloglucan, arabinan, arabinoxylan, and arabinogalactan (as sole carbon sources) on P. copri, single-bacteria fermentation and in vitro fermentation were conducted. Parameters including OD600, SCFAs and P. copri, etc., were systematically measured. In single-bacteria fermentation, the growth of P. copri 2-27 and its acetic acid production were significantly promoted by xylan and arabinan, with OD600 increments of 0.64 and 0.61, and acetic acid concentrations of 10.66 mg·mL-1 and 9.87 mg·mL-1, respectively. During in vitro fermentation, the strongest enrichment capacity for P. copri was seen with arabinan (absolute abundance: 60.63 ng·mL-1), followed sequentially by xylan, arabinogalactan, and arabinoxylan. Notably, arabinan group maintained superior acetic acid and butyric acid production capacities (4.60 mg·mL-1 and 2.78 mg·mL-1, respectively). Although xylan was highly utilized, its P. copri enrichment effect remained limited (6.98 ng·mL-1). Consequently, arabinan was identified as the optimal polysaccharide for promoting P. copri growth and enrichment. This study provided a theoretical reference for exploring the impact of polysaccharides-P. copri interaction on host health.
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Project Supported:
National Science Fund for Excellent Young Scholars (32222065); Key Research and Development Project of Jiangxi Province (20223BBF61023)
