Lilium davidii Improves Liver Circadian Clock Clock-Bmal1 Gene Expression and Glucose and Lipid Metabolism in High-fat Diet Fed Rats
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Abstract:
To explore the regulation and mechanism of Lilium davidii var. willmottiae (LD) on the liver circadian clock gene Clock-Bmal1 and glucose and lipid metabolism in rats on a high-fat diet. The model was established by feeding rats with high-fat diet for 8 weeks. The high-fat rats were randomly divided into the model group, metformin group, melatonin group, low-dose LD group, medium-dose LD group, high-dose LD group, combination group, with the normal group being fed with an ordinary diet. Intragastric intervention was carried out for 4 weeks. Fasting blood glucose (FPG), abdominal circumference, body length, and liver weight were measured. Lee's index and liver wet weight index were calculated. Total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) in the serum and liver were detected by the microplate method. The level of glycosylated hemoglobin (Hb Alc) was detected by ELISA. The mRNA and protein expression levels of the Clock and Bmal1 genes in the liver were detected by RT qPCR and Western blot. The results showed that compared with the model group, the LD intervention group could improve the body posture of high-fat rats, regulate the blood lipid levels, reduce Hb Alc, and upregulate the mRNA and protein expression of Clock and Bmal1 (P<0.05). The abdominal circumference and Lee's index of the medium-dose LD group decreased (8.61%, 5.04%), with their serum TC, TG and LDL-C decreasing (36.59%, 29.73%, 34.04%), Hb Alc decreasing by 24.37%, Clock and Bmal1mRNA expression increasing (159.17%, 228.58%) and protein expression increasing (52.09%, 151.33%). In conclusion, LD alleviated glucose and lipid metabolism disorders caused by a high-fat diet, which might be achieved through the upregulation of the liver Clock and Bmal1 gene expression. The results of this study provide a theoretical basis for using LD in the prevention and treatment of metabolic diseases and the development and utilization of functional health foods.
