Preparation of Sulfated Schizophyllan Polysaccharides with Different Degrees of Substitution and Their Hypoglycemic and Moisturizing Activities
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Abstract:
In this study, Schizophyllan Polysaccharides (SPG) was used as the as raw material, sulfated Schizophyllan polysaccharides (S-SPGs) was prepared by the sulfur trioxide-pyridine method , and the relationship between the degree of substitution (DS) of S-SPGs and their in vitro hypoglycemic and moisturizing activitieswas examined. The experimental results showed that four S-SPGs with a DS of 0.66~1.81 could be obtained sequentially with the increase of the mass fraction of sulfur trioxide-pyridine. The weight-average molecular weight Mw of S-SPGs decreased gradually from 1.67×107 u to 8.04×105 u with the increase of DS. The PDI of S-SPG with a DS of 1.19 was as low as 7.84. Excessive sulfation would lead to disordered degradation of S-SPGs. The results of Congo red experiment showed that there was no triple helix structure in S-SPGs. T The inhibitory rate of the S-SPG with a DS of 1.81 and concentration of 2mg/mL against α-glucosidase could reach 73.8%. All the four S-SPGs were reversible mixed type I inhibitors. The moisture-absorbing and moisture-holding capacities of the SPG after sulfation were superior to those before modification, and the moisture-absorbing capacities of the S-SPG with DS1.81 were 77.93% (RH 43%) and 52.54% (RH 81%), respectively, which were second only to glycerol but better than hyaluronic acid. In dry environments, S-SPGs had slightly worse moisture-holding capacities than sodium hyaluronate. Therefore, S-SPGs had better α-glucosidase-inhibitory activity and moisture-absorbing and moisture-holding capacities, and both of the latter capacities increased with the increase of substitution degree.
