Abstract:Different deer skin gelatin samples of amino acid compositions were investigated and its drug-food homologous prescription was used to study immunomodulatory effects on immune-suppressed mice. Amino acid composition was comprehensively analyzed using an amino acid analyzer. Various parameters, including body weight, immune organ indices, immune factor levels, delayed-type hypersensitivity, serum hemolysin, carbon clearance assay, peritoneal macrophage phagocytosis of chicken red blood cells, and jejunal histopathological changes, were observed in different mouse groups. The results revealed that deer skin gelatin contains 17 amino acids, with a total content ranging from 68.47 to 69.82 g/100 g. Glycine had the highest content (17~18 g/100 g), while cysteine had the lowest (0.15~0.3 g/100 g), with sample 3 scoring the highest. Different doses of deer skin gelatin (0.7, 1.4, and 2.8 g/kg) in traditional medicine formulations significantly increased the thymus index (2.36, 2.47, and 2.51 mg/g), spleen index (5.43, 5.84, and 5.94 mg/g), carbon particle clearance (5.22, 5.30, and 5.66), serum hemolysin levels (47.87, 50.6, and 53.4), macrophage phagocytosis index (0.34, 0.35, and 0.36), phagocytosis rate (43.20%, 44.57%, and 44.87%), and immunological factors IgA (133.40,134.34, and 146.81 pg/mL), IgG (121.83,129.83, and 133.58 pg/mL), IgM (200.44, 204.58, and 214.4 pg/mL), IFN-γ (109.55, 110, and 113.75 pg/mL), TNF-α (173.80,176.79, 179.14 pg/mL), and IL-4 (164.26, 172.04, and 208.85 pg/mL). Meanwhile, ear swelling degree significantly decreased (5.91, 5.46, and 5.55 mg), and ileal mucosal barrier damage was improved. In conclusion, deer skin gelatin possesses rich amino acid content, and its traditional medicine formulations exhibit immunomodulatory effects, which provides new ideas for the development of healthy food.
