Rapid Determination of Bellkute Residues in Foods of Plant Origin by Ultra-Performance Liquid Chromatography-tandem Mass Spectrometry
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Abstract:
A rapid analysis method for the determination of Bellkute residues in foods of plant origin by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established. The unique enhanced resolution scan (ER) mode of QTRAP was used to solve the difficulty of finding the parent (precursor) ions for multi-charged compounds. The effects of pretreatment, consumable selection, and matrix effects on the recovery rate were investigated, and 20% (V/V) methanol aqueous solution [containing 1% (V/V) formic acid] was selected for extraction. Guanidine hydrochloride (0.5 g) was added and polypropylene was used as the experimental material, to reduce the adsorption of the target by plant components and experimental consumables. The Kinetex F5 column was used for separation, and the quantitative analysis was performed in the positive ion and multiple reaction monitoring mode. The results showed that Bellkute had a good linear relationship in the range of 0.1~100.0 μg/L, and the correlation coefficient r was not lower than 0.998. Using nine plantderived foods such as citrus and asparagus and three spiking levels, the average recovery rate of Bellkute was determined to range from 75.2% to 103.5%, with the relative standard deviations (RSD, n=6) being 2.1%~14.3%, and the limit of detection (LOD) and limit of quantification (LOQ) being 0.6 μg/kg and 2.0 μg/kg, respectively. This method can be used for rapid extraction, and qualitative and quantitative detection of Bellkute residues in foods of plant origin, with advantages of simple operation, high sensitivity, and low cost, thereby being suitable for daily screening and confirmation of Bellkute residues in large quantities of foods of plant origin.
