Comparison of Lanzhou Lily Polysaccharides with Gradient Ethanol Precipitation and Their in vitro Digestive Tolerance and Prebiotic Activity
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Abstract:
Lanzhou lily (Lilium davidii var. unicolor Salisb. ), a medicinal and edible plant in China, is commonly referred to as sweet lily. To further investigate the prebiotic potential of one of its major bioactive components, lily polysaccharides, and enhance the utilization of lily resources, a study was conducted using ultrasonic-assisted enzymatic extraction with volume fractions of 60%, 80%, and 95% ethanol to obtain three types of lily polysaccharides (LLP). Their in vitro digestive tolerance and prebiotic activity were examined. The results demonstrated that as the ethanol volume fraction increased, the soluble total sugar content, extraction yield, protein content, and tolerance to simulated gastric and intestinal fluids of Lanzhou lily polysaccharides were all improved. Notably, LLP-95% exhibited a soluble total sugar content and extraction yield of 19.83% and 23.77%, respectively, while the hydrolysis degree in simulated gastric juice after 6 hours was only 5.90%. Additionally, the protein content was relatively low at just 4.09%. When used as a carbon source, Lactobacillus plantarum fermentation for 48 hours resulted in a prebiotic index (PI) of 0.96, pH value of 4.73, and OD600 of 0.75, significantly outperforming the fructooligosaccharides and other polysaccharide groups. Furthermore, after 40 minutes of incubation in simulated gastric and intestinal fluids, the survival rates of bacterial suspensions treated with LLP-95% were 25.89% and 56.00%, respectively, significantly enhancing bacterial survival compared to the other groups. This study demonstrated that LLP-95% possesses good digestive tolerance, promotes the proliferation of Lactobacillus plantarum, and enhances bacterial survival in simulated gastrointestinal environments, indicating strong prebiotic potential. These findings provide scientific evidence for the development of functional foods and pharmaceuticals.
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Project Supported:
The National Natural Science Foundation of China (General Program, Key Program, Major Research Plan), Applied Research and Development Project of Gansu Academy of Sciences, Supported by Gansu Provincial Science and Technology Plan and supported by Gansu Provincial Department of Agriculture and Rural Affairs
