The Composition, Structure of Polysaccharide of Silkworm Pupa and its Application in Emulsions
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Abstract:
The composition, structure, and application of polysaccharide of silkworm pupa (PSP) in emulsions were investigated in this study. PSP was extracted from silkworm pupae and used to prepare high internal phase emulsions (Φ=74%). The composition and structure of PSP were evaluated, and the effects of PSP concentrations on the emulsifying activity index (EAI), emulsion stability index (ESI), Zeta potential, droplet size, creaming index, stability index, and rheological properties of the emulsion were analyzed. The results showed that PSP was an acidic polysaccharide, which primarily composed of fucose, N-acetyl-D-galactosamine hydrochloride, rhamnose, arabinose, N-acetyl-D-glucosamine hydrochloride, galactose, glucose, mannose, and glucuronic acid. The concentrations of PSP significantly influenced the stability of the emulsion. As the PSP concentrations increased from 5 mg/mL to 80 mg/mL, the EAI increased from 3.43 m2/g to 17.24 m2/g, the ESI improved from 10.39 min to 548.3 min, the absolute value of Zeta potential rose from 15.77 mV to 28.1 mV, and the droplet size decreased from 135.8 μm to 62.07 μm. Among them, the emulsion stabilized by 80 mg/mL PSP exhibited the lowest stability index and highest viscosity, exhibiting the best storage stability. This study showed that PSP can stabilized high internal phase emulsion, and increasing its concentration within a certain range (5~80 mg/mL) can improve emulsification performance, making it suitable as an emulsifier and thickener in the food industry.