RRR-Regulation of Lipid Metabolism in Rats by ergosterol-rich Lentinus Edodes stalk extracts
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Abstract:
To determine the effect of ergosterol-rich Lentinus Edodes stalk extracts on lipid metabolism, this study first analyzed the content of ergosterol in the cap and stalk of Lentinula edodes by high performance liquid chromatography, and then further investigated the regulatory effects of the n-hexane extract (SEHE) and methanol/dichloromethane extract (SEMD) of the stalk on serum lipids, liver lipids and fecal lipids in a rat model induced by a high-cholesterol diet. The results showed that the ergosterol content in the cap of Lentinula edodes was 7.82 mg ? g-1, which was significantly higher than that of 5.24 mg ? g-1 in the stalk. Among the two stalk extracts, the ergosterol content of SEHE was 20.31% (W/W), while that of SEMD was 6.12% (W/W). Animal experiments showed that both SEHE and SEMD could significantly reduce the level of low-density lipoprotein cholesterol (LDL-C) in rat serum, with reductions of 51.02% (P < 0.05) and 58.34% (P < 0.05), respectively, and effectively reduce the LDL-C/high-density lipoprotein cholesterol (HDL-C) ratio, with reductions of 54.61% (P < 0.05) and 53.04% (P < 0.05), respectively. These two extracts could also reduce the cholesterol content (-19.31%, P < 0.05; -23.73%, P < 0.05) and triglyceride content (-9.82%, P = 0.11; -24.83%, P < 0.05) in the rat liver. In addition, SEMD could also significantly reduce the total cholesterol (TC) level in rat serum (-13.31%, P < 0.05). The above results indicate that both stalk extracts can effectively regulate lipid metabolism in rats and have the potential to be used as functional food ingredients for the prevention of cardiovascular diseases.
