Optimization of Enzymatic Preparation Process of Antioxidant Peptides from Prinsepia utilis Royle and Analysis of Their in Vitro Antioxidant Activities
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Abstract:
Prinsepia utilis Royle is a food and medicine homologous woody oil crop with Yunnan characteristics. The by-products after oil extraction have the problem of low protein utilization. In this experiment, Prinsepia utilis Royle meal was used as the raw material to produce protein hydrolysate with antioxidant activity. The single factor experiments and response surface optimization analysis were carried out on three factors, enzyme mass fraction, enzymatic hydrolysis time and solid-liquid ratio, to determine the enzymatic hydrolysis process conditions for the preparation of protease hydrolysate from Prinsepia utilis Royle meal by alkaline protease. The results showed that the optimal enzymatic hydrolysis conditions were: enzyme mass fraction, 4.6%; enzymatic hydrolysis time, 4.6 h; solid-liquid ratio, 1:31 g/mL. Under the optimal conditions, when the mass concentration of antioxidant peptide prepared by enzymatic hydrolysis was 0.9 mg/mL, the scavenging capacities for DPPH free radical, ABTS+ free radical and hydroxyl free radical were 76.46%, 94.22% and 73.02%, respectively. In addition, the results of cytotoxicity experiments showed that the peptide had no toxic effect on the growth of HepG 2 cells. Taken together, the antioxidant peptide prepared through this process had good antioxidant activity. This study can provide a reference for the development and utilization of Prinsepia utilis Royle protein resource.
