Skin Barrier Repairing Efficacy and Safety Evaluation of Gastrodia elata Extract
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Abstract:
Gastrodia elata extract (GEE) was prepared using the thermal reflux method and its ability to repair damaged skin barrier was investigated using a HaCaT cell model irradiated with 0.15 J medium-wave ultraviolet B, as well as its moisturizing efficacy was evaluated in humans. At a GEE concentration of 200 μg/mL, the relative cell survival viability was 89.79%, with the inhibition rates of tumor necrosis factor (TNF)-α and prostaglandin E2 (PGE2) being 16.30% and 24.51%, respectively. The reactive oxygen species (ROS) content decreased by 32.70%, thereby upregulating the expression of fillagrin (FLG) and aquaporin 3 (AQP3) mRNA by 65.79% and 189.60%, with the protein contents both increasing by 17.86% and 16.73%, respectively. Moreover, the chick embryo chorionic allantoic membrane assay showed that the GEE samples with a concentration ≤7 mg/mL caused no considerable irritation. Furthermore, the human patch test and moisturizing efficacy evaluation indicated that the gel samples containing 0.5% (mass fraction) GEE caused no adverse reactions on human skin, increased the skin moisture content by 14.28%, and decreased moisture loss by 15.09% compared with that of the blank group. Therefore, GEE exerts barrier repair efficacy by reducing ROS content, inhibiting TNF-α and PGE2 secretion, upregulating AQP3 and FLG mRNA expression, increasing AQP3 and FLG protein content, and improving skin moisture. Owing to its low irritation and high safety, EGG shows promising potential as a soothing and rejuvenating ingredient.
