Ginsenoside Rg2 Alleviates Lipopolysaccharide-induced Inflammation of RAW264.7 Cells by Regulating the PI3K/AKT/NF-κB Signaling Pathway
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Abstract:
The anti-inflammatory effects of ginsenoside Rg2 were investigated based on a bacterial lipopolysaccharide (LPS)-induced inflammation model of RAW264.7 cells. The expression levels of inflammation-related genes and proteins in the model were analyzed using PCR, RT-qPCR, and western blotting. The results demonstrated that ginsenoside Rg2 dosedependently inhibited the release of nitric oxide from inflammatory cells (P<0.05) and transcription levels of inflammationrelated genes, including inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor-α, and interleukin-1β (P<0.05). Scanning electron microscopy analysis showed that ginsenoside Rg2 improved the morphology of inflammatory RAW264.7 cells after LPS induction. Western blotting results revealed that ginsenoside Rg2 inhibited the phosphorylation of PI3K, PDK1, AKT, and GSK-3β and prevented NF-κB from entering the nucleus. These results suggest that ginsenoside Rg2 has an anti-inflammatory effect and can reduce LPS-induced inflammation of RAW264.7 cells via the PI3K/AKT/NF-κB signaling pathway. The findings of this study improve the understanding of the anti-inflammatory activity of ginsenoside Rg2 and associated mechanism and provide a foundation for the development of related functional foods.
